Abstract: A method to improve the effectiveness of MEOR or bioremediation processes has been disclosed. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.

Abstract: A method to improve the effectiveness of MEOR or bioremediation processes has been disclosed. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.

Abstract: A method to improve the effectiveness of MEOR or bioremediation processes has been disclosed. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.

Abstract: A method to improve the effectiveness of MEOR or bioremediation processes has been disclosed. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.

Abstract: A method to improve the effectiveness of MEOR or bioremediation processes has been disclosed. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.

Abstract: A method to improve the effectiveness of MEOR or bioremediation processes has been disclosed. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.

Abstract: A method of increasing biogenic production of a combustible gas from a subterranean geologic formation is described. The method may include extracting formation water from the geologic formation, where the extracted formation water includes at least a first species and a second species of microorganism. The method may also include analyzing the extracted formation water to identify the first species of microorganism that promotes the biogenic production of the combustible gas. An amendment may be introduced to the formation water to promote the growth of the first species of microorganism, and the biological characteristics of the formation water may be altered to decrease a population of the second species in the geologic formation.

Abstract: The present invention describes methods of identifying stimulants for the biogenic production of methane in hydrocarbon-bearing formations. Methods involve the use of microbial nucleic acid sequence information for the determination of gene products that are enzymes in a variety of pathways involved in the conversion of hydrocarbons to methane. Enzymes and stimulants identified by invention methods can be used in processes for enhancing biogenic methane production, for example, by addition to coal seams and coalbed methane wells.

Abstract: A braking device for drilling operations in a borehole includes a brake retainer having a plurality of brake connector openings defined therein, a body member having a tapered surface having a first diameter and a second diameter, the second diameter being larger than the first diameter, at least one brake element positioned at least partially between the brake retainer and the body member and in communication with the tapered surface and at least one of the brake connector openings, and a bias member configured to exert a biasing force on the body member to move the body member toward the brake retainer to move the brake element from contact with the first diameter of the tapered surface toward contact with the second diameter.

Abstract: A method for stimulating methane production from a carbonaceous material is described. The methods may include the step of contacting the material with cells of a methanogenic consortium under anaerobic conditions to form a reaction mixture. The method may also include maintaining anaerobic conditions for a time sufficient to permit methanogenesis, and collecting methane from anaerobic water or head space of the reaction mixture.

Abstract: Improvement in the compatibility of hydrocarbon and water is achieved by surface active agents newly synthesized by mixed microbial populations. Methods for enhancing the microbial production of surface-active agents in hydrocarbon-exposed surface waters can be achieved by supplementing the mixed microbial population with one or more carbon sources, most notably propionate. Hydrocarbon-exposed surface waters having enhanced levels of surface-active agents can be used to improve hydrocarbon recovery from subterranean formations or for remediation of hydrocarbon-contaminated sites.

Abstract: The present invention describes methods of identifying stimulants for the biogenic production of methane in hydrocarbon-bearing formations. Methods involve the use of microbial nucleic acid sequence information for the determination of gene products that are enzymes in a variety of pathways involved in the conversion of hydrocarbons to methane. Enzymes and stimulants identified by invention methods can be used in processes for enhancing biogenic methane production, for example, by addition to coal seams and coalbed methane wells.

Abstract: A method of increasing biogenic production of a combustible gas from a subterranean geologic formation is described. The method may include extracting formation water from the geologic formation, where the extracted formation water includes at least a first species and a second species of microorganism. The method may also include analyzing the extracted formation water to identify the first species of microorganism that promotes the biogenic production of the combustible gas. An amendment may be introduced to the formation water to promote the growth of the first species of microorganism, and the biological characteristics of the formation water may be altered to decrease a population of the second species in the geologic formation.

Abstract: Methods of stimulating biogenic production of a metabolic product with enhanced hydrogen content are described. The methods may include accessing a consortium of microorganisms in a geologic formation that includes a carbonaceous material. They may also include providing a phosphorous compound to the microorganisms. The phosphorous compound stimulates the consortium to metabolize the carbonaceous material into a metabolic product with enhanced hydrogen content. Also, methods of stimulating biogenic production of a metabolic product with enhanced hydrogen content by providing a yeast extract amendment to a consortium of microorganisms is described. The yeast extract amendment stimulates the consortium to metabolize carbonaceous material in the formation into the metabolic product with enhanced hydrogen content.

Abstract: The present invention refers to a biotechnological process that enhances oil recovery of a 14 to 25 API Gravity oil contained in carbonate-containing and/or clayey sandstone porous rock systems with low permeability (7 to 100 mD), thus focused to petroleum wells associated to zones with low recovery factor. The process utilizes the indigenous extremophile microorganisms activity from the oil reservoir and an IMP culture, as well as its metabolites (gases, acids, solvents and surfactants), which improve oil mobility and are able to develop at 60 to 95° C. temperatures, 7 to 154.6 Kg/cm2 (100 to 2,200 psi) pressures and NaCl content from 5,000 to 45,000 ppm, in anaerobic conditions.

Abstract: This invention provides a method of controlling water in a subterranean formation, said method comprising contacting said formation with a water control treatment agent which comprises an organosilane in an amount effective to reduce the water permeability of at least part of said formation. At the same time, any decrease in the overall hydrocarbon (e.g. oil) permeability of said formation is preferably minimized. The invention further provides a method of plugging or sealing a subterranean formation.

Abstract: Methods to enhance biogenic gas production in an anaerobic geologic formation containing carbonaceous material are described. The methods may include the steps of accessing the anaerobic formation, increasing a rate of production of the biogenic gases in the anaerobic formation, and flowing formation water within the anaerobic formation after the increase in the production of biogenic gases. Also described are methods to redistribute formation water in an anaerobic geologic formation containing carbonaceous material. The methods may include the steps of locating a reservoir of the formation water within the anaerobic formation, forming at least one channel between the reservoir and at least a portion of the carbonaceous material, and transporting the formation water from the reservoir to the carbonaceous material through the channel. Further described are methods of accumulating biogenic gas in an anaerobic geologic formation to enhance biogenic gas production.

Abstract: Apparatus and methods of reducing the viscosity of a gelled fluid is provided. In one embodiment, a viscosity reducing microbe is disposed in a capsule and added to the gelled fluid. The gelled fluid may include a thickening agent adapted to increase its viscosity. Upon release from the capsule, the microbe begins to digest the thickening agent in the gelled fluid and/or releases enzymes that that breakdown the thickening agent.

Abstract: The present invention is directed to the field of microbial enhanced oil recovery (MEOR). In particular, the invention focuses on new, efficient, economical and environmentally safe microbial methods to enhance oil recovery in existing oil reservoirs, as well as microorganisms useful in such methods.

Abstract: The present invention is generally directed to a method for releasing trapped oil in reservoirs. The method hereof includes identifying a reservoir, obtaining a microbial community sample of the reservoir, maintaining the sample under high temperature and high pressure conditions that mimic natural conditions of the reservoir, growing the sample on at least one substrate, determining a targeted treatment regime for the reservoir based on the positive growth of the sample on the substrate, injecting the reservoir with the targeted substrate to release trapped oil in the reservoir, monitoring the reservoir, and extracting the oil from the reservoir.

Abstract: Changing concentrations of brine in a gravel pack carrier fluid gelled with a viscoelastic surfactant (VES) increases the fluid efficiency for gravel packing long interval wells, such as wellbore producing interval greater than about 100 feet (about 30 m). VES-gelled fluids used as gravel packing fluids herein also include surfactants, fluid loss control agents, internal breakers and brine in addition to the grave. The viscoelasticity of fluid system can suspend and deliver high concentration of the gravels while reducing carrier fluid volume.

Abstract: A process for introducing microorganisms to carbonaceous material in an anaerobic environment is described. The process includes the step of extracting formation water from a geologic formation, and removing at least a portion of an extractable material from the formation water to make amended formation water. The process may further include introducing the amended formation water to the carbonaceous material. Also, a process for increasing biogenic hydrocarbon production in a geologic formation containing a carbonaceous material is described. The process includes extracting formation water from the formation, removing at least a portion of one or more hydrocarbons from the formation water to make amended formation water, and reintroducing the amended formation water to the geologic formation.

Abstract: A method of increasing biogenic production of a combustible gas from a subterranean geologic formation is described. The method may include extracting formation water from the geologic formation, where the extracted formation water includes at least a first species and a second species of microorganism. The method may also include analyzing the extracted formation water to identify the first species of microorganism that promotes the biogenic production of the combustible gas. An amendment may be introduced to the formation water to promote the growth of the first species of microorganism, and the biological characteristics of the formation water may be altered to decrease a population of the second species in the geologic formation.

Abstract: Systems for enhanced in-situ or perhaps even ex-situ biogenic methane production from hydrocarbon-bearing formations (1) including coal seam, oil shale, coal, coal derivates and the like are presented in embodiments such as but not limited to: increasing and perhaps even selection of microbial populations (2), amending CBM water and other microbe-containing media, diminishing sulfate reduction competition, enhancing organic matter concentrations and generation of biogenic methane (10), universally treating hydrocarbon-bearing formations, and introducing amendments (3) to hydrocarbon-bearing formations.

Abstract: An electric plasma arc apparatus and method produces nitrogen compounds, solely using ambient air extracted in proximity to the apparatus. The nitrogen compounds are brought into contact with a water processing system, forming nitrate on-site. Hydrogen sulfide present in the water processing system is removed, and the production of hydrogen sulfide by sulfate-reducing bacteria (SRB) is eliminated by introducing nitrate into the system, whereby denitrifying microorganisms, using the nitrate, outcompete the sulfate-reducing bacteria for the available carbon nutrients, thus preventing the SRB from producing hydrogen sulfide. Nitrate ions generated in the water processing system which contains the denitrifying microorganisms can enhance oil recovery by means of microbial enhanced oil recovery mechanisms.

Abstract: Methods and compositions are provided for increasing methane gas produced or released from a subsurface hydrocarbon containing formation containing methanogenic microorganisms. The methods and compositions provide using an amount of amino acids effective to increase methane gas released from the formation.

Abstract: The present disclosure relates to developing an enriched steady state microbial consortium capable of modifying crude oil components of an oil reservoir under anaerobic denitrifying conditions. The steady state consortium may be used to improve oil recovery.

Abstract: Remote operation wire line core sampling device capable of increasing efficiency of working and maintaining a wall of a hole using a stabilized sea-bottom core drill at a sea-bottom, and adopting a wire line system. The device includes a drill rod held by a chuck of the sea-bottom core drill, a wire line core barrel having, at a tip thereof, a bit for annularly drilling into ground, an inner tube assembly detachably incorporated in the wire line core barrel, and an over-shot assembly having a function for engagement thereof with a spear head part at a top end of an inner tube assembly. The device also includes a water swivel in which water supply ports are formed at upper and lower parts thereof, a modified built-in type over-shot assembly is formed at upper and lower parts thereof, and a modified built-in type over-shot assembly is incorporated therein to reside at an intermediate part thereof.

Abstract: A method for the phylogenetic identification of indigenous anaerobic denitrifying bacterial species in an oil well is described. The method avoids the process of enrichment of microbial samples.

Abstract: The invention relates to treating of subterranean formations to increase the susceptibility (by way of chemical break down or solubilization, but not necessarily limited by these or any other theories) of large carbonaceous molecules therein, such as comprise coal, to bioconversion into methane and other useful hydrocarbon products by indigenous and/or non-indigenous microbial consortia, by introducing into the subterranean formations: (a) a solution containing at least one of an oxoacid ester of phosphorus or a thioacid ester of phosphorus; and (b) one or more other chemical compounds/chemical entities selected from the group consisting of: hydrogen, carboxylic acids, esters of carboxylic acids, salts of carboxylic acids, oxoacids of phosphorus, salts of oxoacids of phosphorus, vitamins, minerals, mineral salts, metals, and yeast extracts.

Abstract: Viscoelastic surfactant (VES) gelled aqueous fluids containing water, a VES, an internal breaker, a VES stabilizer, a fluid loss control agent and a viscosity enhancer are useful as treating fluids and particularly as fracturing fluids for subterranean formations. These VES-based fluids have faster and more complete clean-up than polymer-based fracturing fluids. The use of an internal breaker permits ready removal of the unique VES micelle based pseudo-filter cake with several advantages including reducing the typical VES loading and total fluid volume since more VES fluid stays within the fracture, generating a more optimum fracture geometry for enhanced reservoir productivity, and treating reservoirs with permeability above the present VES limit of approximately 400 md to at least 2000 md.

Abstract: The present disclosure relates to isolation, identification and application of Thauera strain AL9:8 which grows on crude oil as the sole carbon source under denitrifying anaerobic conditions Thauera strain AL9:8 can be used alone or in concert with other microorganisms to improve oil recovery, bioremediation of oil or hydrocarbons in contaminated soil, ground water or bodies of water, such as lakes, rivers or oceans and/or applying to method for promoting oil pipeline maintenance, removing a build up hydrocarbon on the intersurfaces of the pipeline.

Abstract: A method for stimulating methane production from a carbonaceous material is described. The methods may include the step of contacting the material with cells of a methanogenic consortium under anaerobic conditions to form a reaction mixture. The method may also include maintaining anaerobic conditions for a time sufficient to permit methanogenesis, and collecting methane from anaerobic water or head space of the reaction mixture.

Abstract: Methods of stimulating biogenic production of a metabolic product with enhanced hydrogen content are described. The methods may include accessing a consortium of microorganisms in a geologic formation that includes a carbonaceous material. They may also include providing hydrogen and one or more phosphorous compounds to the microorganisms. The combination of the hydrogen and phosphorous compounds stimulates the consortium to metabolize the carbonaceous material into the metabolic product with enhanced hydrogen content. Also, methods of stimulating biogenic production of a metabolic product with enhanced hydrogen content by providing a carboxylate compound, such as acetate, to a consortium of microorganisms is described. The carboxylate compound stimulates the consortium to metabolize carbonaceous material in the formation into the metabolic product with enhanced hydrogen content.

Abstract: The present disclosure relates to isolation, identification and application of Thauera strain AL9:8 which grows on crude oil as the sole carbon source under denitrifying anaerobic conditions Thauera strain AL9:8 can be used alone or in concert with other microorganisms to improve oil recovery, bioremediation of oil or hydrocarbons in contaminated soil, ground water or bodies of water, such as lakes, rivers or oceans and/or applying to method for promoting oil pipeline maintenance, removing a build up hydrocarbon on the intersurfaces of the pipeline.

Abstract: The invention provides a composition having shear-gelling and shear-thickening properties based on a amphiphilic polymer combined with hydrophilic particles and polyethyleneoxide.

Abstract: A process to increase the area of microbial stimulation in a process of methane gas recovery in a multi seamed coal bed/methane dewatering and depressurizing production system by first utilizing an underbalanced multilateral drilling technique. At this point in the process one could introduce the microorganisms into the horizontal well bore to achieve a greater area of stimulation of the coal bed than would a vertical well. An even more preferred method would to first drill a series of lateral wells off of the horizontally drilled well bore, so as to increase or maximize the area of coal bed which is being covered. At that point, one would take the steps of what is known in the art of introducing a particular type of microorganism, which would then be injected via the plurality of lateral bores into the coal bed formation, to maximize the area of penetration, which would include, most, if not all, of the area of the coal bed through the series of lateral wells.

Abstract: A method of reducing wax deposition in an oil wellbore tubing includes providing an aqueous bioslurry comprising at least one microbe capable of degrading paraffin wax at about 20-45° C. and a nutrient medium containing one or more carbon source and an oxygen slow-releasing compound. The method further includes providing an oil wellbore completion having a production tubing with an interior surface including a zone that is susceptible to wax deposition when the well is operated to produce oil. The method further includes, while oil production from the well is stopped, incubating the biological slurry in the wax-deposition zone tubing for a sufficient time to multiply the microbe(s) and form a biofilm on at least the wax-susceptible zone.

Abstract: Methods of reducing the viscosity of crude oil in an oil well are provided. The methods comprise the introduction of at least on reductant to an injection fluid which is then injected into the well. Aromatic hydrocarbons in the oil are reduced in the presence of reductant and indigenous microbial populations to reduce the viscosity of the oil.

Abstract: An oil extraction system is provided. The oil extraction system includes a sucker rod pump (10) provided for a production well (2) so as to pump up crude oil; a signal detecting section (12) for detecting a pump-off signal sent from the sucker rod pump (10); and an injection pump (20) provided for an injection well (3) so as to pressure-inject fluid which is used for secondary extraction or tertiary extraction. The pressure injection pump (20) is operated based on the pump-off signal detected by the signal detecting section (12).

Abstract: A method for the phylogenetic identification of indigenous anaerobic denitrifying bacterial species in an oil well is described. The method avoids the process of enrichment of microbial samples.

Abstract: A method for environmental monitoring and bioprospecting includes the steps of: (a) utilizing a testing device having: (i) a container having a fluid inlet and outlet, (ii) a plurality of capillary microcosms situated within the container, each of these capillaries having an inlet and outlet that are configured so as to allow for fluid flow through the capillaries, each of these capillaries further having a means for covering its inlet and outlet so as to prevent flow through the capillary, (iii) a pump connected to the container outlet, the pump being configured so as to draw fluid from the surrounding environment into the container's inlet and through the capillaries, (iv) connected to the outlet of the container, a means for collecting the flow through the container, and (v) a check valve connected downstream of the container to prevent the backflow of fluid into the container, (b) adding specified test substances to the device's capillaries, wherein these substances are to be analyzed for their ability to

Abstract: A method for stimulating methane production from a carbonaceous material is described. The methods may include the step of contacting the material with cells of a methanogenic consortium under anaerobic conditions to form a reaction mixture. The method may also include maintaining anaerobic conditions for a time sufficient to permit methanogenesis, and collecting methane from anaerobic water or head space of the reaction mixture.

Abstract: A process to increase the area of microbial stimulation in a process of methane gas recovery in a multi seamed coal bed/methane dewatering and depressurizing production system by first utilizing an underbalanced multilateral drilling technique. At this point in the process one could introduce the microorganisms into the horizontal well bore to achieve a greater area of stimulation of the coal bed than would a vertical well. An even more preferred method would to first drill a series of lateral wells off of the horizontally drilled well bore, so as to increase or maximize the area of coal bed which is being covered. At that point, one would take the steps of what is known in the art of introducing a particular type of microorganism, which would then be injected via the plurality of lateral bores into the coal bed formation, to maximize the area of penetration, which would include, most, if not all, of the area of the coal bed through the series of lateral wells.

Abstract: The present disclosure relates to isolation, identification and application of Shewanella putrefaciens strain LH4:18 that grows, under denitrifying anaerobic conditions, on crude oil as the sole carbon source. This organism assists in oil release from substrate in reservoir simulations when grown on either lactate or peptone as a carbon source. Shewanella putrefaciens strain LH4:18 can be used alone or in concert with other microorganisms to improve oil recovery.

Abstract: Systems for enhanced in-situ or perhaps even ex-situ biogenic methane production from hydrocarbon-bearing formations (1) including coal seam, oil shale, coal, coal derivates and the like are presented in embodiments such as but not limited to: increasing and perhaps even selection of microbial populations (2), amending CBM water and other microbe-containing media, diminishing sulfate reduction competition, enhancing organic matter concentrations and generation of biogenic methane (10), universally treating hydrocarbon-bearing formations, and introducing amendments (3) to hydrocarbon-bearing formations.

Abstract: This invention deals with a method for increasing the recovery factor in oil reservoirs through the injection of polymerization precursor liquids with low viscosity for the production of biopolymers in situ. The method acts in conjunction in the injection profile correction and advanced oil recovery. The invention also refers to the composition of liquids that are precursors of polymerization with low viscosity and to the use of spores or specific bacterium for said purpose. A polymerization precursor liquid is injected into the reservoir which impregnates the rocky matrix. Afterwards, a continuous injection of a polymerizable liquid with additives of low viscosity is performed. The creation of a biopolymer occurs in situ, adhering itself to the rocky matrix, preferably in the thief zones, blocking said zones.

Abstract: A method for converting coal to biogenic methane is provided. The method comprises providing a solid coal matrix, converting the solid coal matrix into soluble, coal-derived constituents, introducing indigenous microorganisms and nutrients into the soluble, coal-derived constituents, and biologically converting the soluble, coal-derived constituents into methane.

Abstract: Viscoelastic surfactant (VES) gelled aqueous fluids containing a VES, an internal breaker, and optionally a viscosity enhancer are useful as diverting fluid for directing placement of an acid into a subterranean formation, where the acid is injected subsequent to introducing the VES gelled fluid. These VES-based diverting fluids have faster and more complete clean-up than polymer-based diverting fluids. The viscosity enhancers may include pyroelectric particles and/or piezoelectric particles. The VES gelled fluid may optionally contain a fluid loss agent which increases the viscosity of the fluid and/or facilitates development of an external viscous VES fluid layer (e.g. a pseudo-filter cake) on the formation face. The VES gelled fluid may also optionally contain an agent that stabilizes the viscosity of the fluid, for instance at high temperatures, such as MgO, Mg(OH)2, CaO, Ca(OH)2, NaOH, and the like.

Abstract: The present invention relates to methods for improving hydrocarbon and water compatibility. More specifically, the invention relates to methods for enhancing the microbial production of surface-active agents in hydrocarbon-exposed surface waters in order to improve hydrocarbon and water compatibility. Hydrocarbon-exposed surface waters having enhanced levels of surface-active agents can be used to improve hydrocarbon recovery from subterranean formations or for remediation of hydrocarbon-contaminated sites.