With Detailed Detection Patents (Class 204/452)
  • Patent number: 10787660
    Abstract: Various aspects of the present disclosure are directed toward methods and apparatuses for interacting a first liquid and a second liquid in one or more fluidic channels of a capillary structure. The methods and apparatuses can include providing at least one capillary barrier that positions a meniscus of the first liquid at a fluid-interface region using capillary forces within the capillary structure. Additionally, a path is provided along one of the channels for the second liquid to flow toward the fluid-interface region. Additionally, gas pressure is released, via a gas-outflow port, from the fluid-interface region while flow of the first liquid is arrested. Further, the first liquid and the second liquid contact in the fluid-interface region with the capillary barrier holding the first liquid at the fluid-interface region.
    Type: Grant
    Filed: November 15, 2018
    Date of Patent: September 29, 2020
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Juan G. Santiago, Lewis A. Marshall, Anita Rogacs
  • Patent number: 10761054
    Abstract: Systems and methods are provided for improving the analysis of analytes by using electrophoresis apparatus. Exemplary methods provide an increase in the yield of useful results, e.g., quantity and quality of useable data, in automated peak detection, in connection with an electrophoretic separation, e.g., capillary electrophoresis. In various embodiments, the system virtualizes the raw data, transforming the migration time into virtual units thereby allowing the visual comparison of analyte electropherograms and the reliable measurement of unknown analytes. The analytes can be, for example, any organic or inorganic molecules, including but not limited to nucleic acids (DNA, RNA), proteins, peptides, glycans, metabolites, secondary metabolites, lipids, or any combination thereof. Analyte detection can be performed by any method including, but not limited to, fluorescence detection or UV absorption.
    Type: Grant
    Filed: May 15, 2017
    Date of Patent: September 1, 2020
    Assignee: Agilent Technologies, Inc.
    Inventors: Bruce DeSimas, Boris Belopolski
  • Patent number: 10753928
    Abstract: Provided is a method for detecting a specific protein, having excellent reliability. The method is characterized by including a mass spectrometry step of detecting the specific protein by mass spectrometry and an immunoassay step of detecting the specific protein by immunoassay using an anti-peptide antibody specifically bonded to the specific protein.
    Type: Grant
    Filed: November 4, 2016
    Date of Patent: August 25, 2020
    Assignee: Morinaga Institute of Biological Science, Inc.
    Inventors: Masahiro Shoji, Tsutomu Honjo, Hideki Okada, Kei Kurihara
  • Patent number: 10746687
    Abstract: The disclosure provides target component measurement methods and target component measurement apparatus for measuring target components including glucose.
    Type: Grant
    Filed: December 1, 2016
    Date of Patent: August 18, 2020
    Assignee: ARKRAY, Inc.
    Inventor: Hisashi Kaneda
  • Patent number: 10656087
    Abstract: The present disclosure relates to a device for measuring an optical absorption property of a fluid as function of wavelength. The device comprises a broadband light source for emitting light, a plurality of integrated optical waveguides for guiding this light, and a light coupler for coupling the emitted light into the integrated optical waveguides such that the light coupled into each integrated optical waveguide has substantially the same spectral distribution. The device also comprises a microfluidic channel for containing the fluid, arranged such as to allow an interaction of the light propagating through each waveguide with the fluid in the microfluidic channel. Each integrated optical waveguide comprises an optical resonator for filtering the light guided by the waveguide according to a predetermined spectral component. The spectral component corresponding to each waveguide is substantially different from the spectral component corresponding to another of the waveguides.
    Type: Grant
    Filed: June 30, 2016
    Date of Patent: May 19, 2020
    Assignee: IMEC VZW
    Inventor: Xavier Rottenberg
  • Patent number: 10652444
    Abstract: Certain embodiments pertain to Multiplexed Fourier Ptychographic imaging systems and methods. In one example, the Multiplexed Fourier Ptychographic imaging system includes an LED array configured to illuminate a sequence of LED patterns for illuminating a sample being imaged. The system includes LED circuitry configured to independently control power to turn on multiple LEDs simultaneously in each LED pattern of the array. The system has a light detector that acquires a first set of lower resolution images of the sample each image acquired during exposure time during illumination by a unique LED pattern. The system uses the first set of lower resolution images to generate a second set of lower resolution images associated with each LED in the LED array and iteratively updates overlapping regions in the Fourier domain with the second set of lower resolution images to generate a higher resolution image.
    Type: Grant
    Filed: December 4, 2015
    Date of Patent: May 12, 2020
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Roarke W. Horstmeyer, Guoan Zheng, Changhuei Yang
  • Patent number: 10627375
    Abstract: An electrospray emitter assembly for interfacing a separation column to a mass spectrometer is disclosed. An emitter capillary includes an inlet end and an outlet end. A fitting is coupled to the inlet end of the emitter, configured to be removably connected to the separation column. A stop with a defined through hole is integrated proximate the inlet end of the emitter to produce a path for liquid to flow from the separation column to the emitter via the through hole where a voltage is applied to the liquid entering the emitter.
    Type: Grant
    Filed: July 13, 2017
    Date of Patent: April 21, 2020
    Assignees: Thermo Hypersil-Keystone LLC, Dionex Corporation
    Inventors: Brandon Howard Robson, Xuefei Sun, Xiaodong Liu
  • Patent number: 10514333
    Abstract: The present disclosure describes a device for measuring an optical absorption property of a fluid as function of wavelength. The device comprises a broadband light source for emitting light, a plurality of integrated optical waveguides for guiding this light and a light coupler for coupling the emitted light into the integrated optical waveguides such that the light coupled into each integrated optical waveguide has substantially the same spectral distribution. The device also comprises a microfluidic channel for containing the fluid, arranged such as to allow an interaction of the light propagating through each waveguide with the fluid in the microfluidic channel, and a plurality of spectral analysis devices optically coupled to corresponding waveguides—such as to receive the light after interaction with the fluid. The spectral analysis devices are adapted for generating a signal representative of a plurality of spectral components of the light.
    Type: Grant
    Filed: June 30, 2016
    Date of Patent: December 24, 2019
    Assignee: IMEC VZW
    Inventor: Xavier Rottenberg
  • Patent number: 10514360
    Abstract: Methods, devices, and systems for improving the quality of electrospray ionization mass spectrometer (ESI-MS) data are described, as are methods, devices, and systems for achieving improved correlation between chemical separation data and mass spectrometry data.
    Type: Grant
    Filed: August 6, 2019
    Date of Patent: December 24, 2019
    Assignee: INTABIO, INC.
    Inventors: Erik Gentalen, Steve Lacy, Scott Mack, Luc Bousse, Morten Jensen
  • Patent number: 10481157
    Abstract: An implantable diagnostic device in accordance with the present disclosure provides various benefits such as a compact size thereby allowing implanting of the device inside animate objects; low cost due to incorporation of inexpensive detection circuitry and the use of conventional IC fabrication techniques; re-usability by heating thereby allowing multiple diagnostic tests to be performed without discarding the device; and a configuration that allows performing of simultaneous and/or sequential diagnostic tests for detecting one or more similar or dissimilar target molecules concurrently or at different times.
    Type: Grant
    Filed: November 30, 2018
    Date of Patent: November 19, 2019
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Axel Scherer, Samuel Njoroge, Jingqing Huang
  • Patent number: 10464067
    Abstract: Air-matrix digital microfluidics (DMF) apparatuses and methods of using them to prevent or limit evaporation and surface fouling of the DMF apparatus. In particular, described herein are air-matrix DMF apparatuses and methods of using them in which a separate well that is accessible from the air gap of the DMF apparatus isolates a reaction droplet by including a cover to prevent evaporation. The cover may be a lid or cap, or it may be an oil or wax material within the well. The opening into the well and/or the well itself may include actuation electrodes to allow the droplet to be placed into, and in some cases removed from, the well. Also described herein are air-matrix DMF apparatuses and methods of using them including thermally controllable regions with a wax material that may be used to selectively encapsulate a reaction droplet in the air gap of the apparatus.
    Type: Grant
    Filed: June 6, 2016
    Date of Patent: November 5, 2019
    Assignee: mirOculus Inc.
    Inventors: Mais J. Jebrail, Irena Barbulovic-Nad, Lorenzo Gutierrez, Foteini Christodoulou
  • Patent number: 10447999
    Abstract: In one example implementation, a system for aligning of images of a three-dimensional (3D) object includes a select engine to select a first image and a second image from a sequence of images of a 3D object taken at different object orientations from a capture cycle of a scanner, a combine engine to combine first metadata from the first image and second metadata from the second image to form combined metadata, an orientation engine to select a third image from the sequence of images, where the third image is from an opposite object orientation relative to a first object orientation of the first image or a second object orientation of the second image, and an align engine to align the sequence of images based on the combined metadata and third metadata from the third image.
    Type: Grant
    Filed: October 20, 2015
    Date of Patent: October 15, 2019
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Diogo S. de Lima, Divya Sharma, Dante Dibenedetto, Ilya Gerasimets, Arsenii Saranin
  • Patent number: 10360422
    Abstract: In some aspects, a colored coded analyte sensor is provided. The analyte sensor has a body and a plurality of machine-readable colored codes associated with the body. The colored codes may contain red, green, and blue hues, which are discernable into coded information. The coded information may include the analyte sensor model, analyte sensor calibration constant, expiration or manufacture date of the analyte sensor, analyte sensor counterfeiting codes, warnings, messages to the user, etc. Colored code reading systems, apparatus and methods for reading such color-coded information associated with the analyte sensor are provided, as are numerous other aspects.
    Type: Grant
    Filed: June 14, 2016
    Date of Patent: July 23, 2019
    Assignee: Ascensia Diabetes Care Holdings AG
    Inventors: Paul M. Ripley, Xin Wang, Narasinha Parasnis, Swetha Chinnayelka
  • Patent number: 10354852
    Abstract: A probe assembly is disclosed comprising an inlet for receiving an eluent from a chromatography device; an outlet for delivering the eluent to an ion source of a mass spectrometer; and an attachment device for attaching the outlet to the mass spectrometer. The outlet comprises an electrically conductive capillary and an electrically conductive member surrounding at least part of the electrically conductive capillary. The electrically conductive member is arranged to receive a voltage upon connection of the attachment device to the mass spectrometer and the electrically conductive member is arranged to provide an electrical connection from the electrically conductive member to the electrically conductive capillary.
    Type: Grant
    Filed: July 25, 2018
    Date of Patent: July 16, 2019
    Assignee: MICROMASS UK LIMITED
    Inventors: Steve O'Brien, Ian Trivett
  • Patent number: 10288606
    Abstract: The present invention provides an analysis method for simultaneously detecting or quantifying plural species of target substances by measuring the light emission from plural carriers within a single container from outside of the container, these carriers comprising a plurality of substances that are respectively immobilized thereon for capturing the plural species of target substances, wherein the light emission from a desired carrier is selectively received by measuring the emission from the carriers through an optical filter.
    Type: Grant
    Filed: October 21, 2013
    Date of Patent: May 14, 2019
    Assignee: UNIVERSAL BIO RESEARCH CO., LTD.
    Inventors: Takaki Sugimoto, Tetsuya Ueda, Shuichi Kobayashi, Hideji Tajima
  • Patent number: 10247699
    Abstract: The invention describes a detection system (1) for analytical separation processes, particularly for electrophoresis, characterized by an optoelectronic sensor layer (5) made from organic semiconductor materials extending along the carrier layer (2) for the sample to be tested, for detecting the separated sample.
    Type: Grant
    Filed: November 21, 2007
    Date of Patent: April 2, 2019
    Assignee: ASMAG-Holding GmbH
    Inventor: Max Sonnleitner
  • Patent number: 10209216
    Abstract: The present invention provides an electrospray ionization source, which includes: a capillary, including a spray tip; a first electrode which provides the spray tip with a spray voltage; and a second electrode. The electrical potential difference between the first electrode and the second electrode forms a separation electric field, which allows the electric field separation and electrospray ionization of the sample to be accomplished simultaneously, thereby improving the sensitivity of detection.
    Type: Grant
    Filed: July 22, 2016
    Date of Patent: February 19, 2019
    Assignee: BEIJING INSTITUTE OF TECHNOLOGY
    Inventors: Wei Xu, Zezhen Zhang, Muyi He, Lili Hu
  • Patent number: 10175235
    Abstract: An implantable diagnostic device in accordance with the present disclosure provides various benefits such as a compact size thereby allowing implanting of the device inside animate objects; low cost due to incorporation of inexpensive detection circuitry and the use of conventional IC fabrication techniques; re-usability by heating thereby allowing multiple diagnostic tests to be performed without discarding the device; and a configuration that allows performing of simultaneous and/or sequential diagnostic tests for detecting one or more similar or dissimilar target molecules concurrently or at different times.
    Type: Grant
    Filed: May 17, 2016
    Date of Patent: January 8, 2019
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Axel Scherer, Samuel Njoroge, Jingqing Huang
  • Patent number: 10126264
    Abstract: Devices, systems, and methods are disclosed that analyze a biological or other fluid sample using an electrophoresis or other separation method and then emit the fluid sample with separated constituents using an electrohydrodynamic spray to form a Taylor cone and jet, without dispersion into droplets, onto a substrate that moves with respect to the emitter. Electrodes can be shared between the electrophoresis and electrospray elements, and an adjunct fluid can help draw the separated sample into the Taylor cone. A micro-machined capillary channel on a chip can supply multiple lines to a substrate.
    Type: Grant
    Filed: July 2, 2015
    Date of Patent: November 13, 2018
    Assignee: LI-COR, Inc.
    Inventor: Michael D. Furtaw
  • Patent number: 10107782
    Abstract: A method and apparatus are provided for performing capillary isoelectric focusing followed by mobilization of the focused zones by induced hydrodynamic flow or chemical mobilization. These two dimensions of separation are integrated with real-time whole-channel electrophoresis detection and automatic sample injection to achieve a separation resolution superior to that obtainable using known orthogonal capillary two dimensional arrangements.
    Type: Grant
    Filed: January 23, 2009
    Date of Patent: October 23, 2018
    Assignee: ProteinSimple
    Inventors: Tiemin Huang, Jiaqi Wu
  • Patent number: 10067118
    Abstract: A single molecule filter includes: a membrane including: a first surface; a second surface; and a membrane aperture disposed in the membrane and traversing the membrane from the first surface to the second surface, the membrane aperture provided to communicate a single molecule across the membrane; and a nanotube disposed on the membrane and including: a first end disposed on the first surface of the membrane; a second end disposed distal to the first surface; and a tubular aperture extending along the nanotube from the first end to the second end, the tubular aperture provided to communicate the single molecule from the second end of the nanotube to the membrane aperture.
    Type: Grant
    Filed: September 30, 2015
    Date of Patent: September 4, 2018
    Assignee: NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY
    Inventors: John S. Suehle, John J. Kasianowicz, Arvind Balijepalli, Joseph W. Robertson, Jessica Benjamini
  • Patent number: 10060889
    Abstract: A system and method for performing UV LED-based absorption detection for capillary liquid chromatography for detecting and quantifying compounds in a liquid, wherein a simplified system eliminates the need for a beam splitter and a reference cell by using a stable UV source, and power requirements are reduced, resulting in a portable and substantially smaller system with relatively low detection limits.
    Type: Grant
    Filed: May 15, 2015
    Date of Patent: August 28, 2018
    Assignee: BRIGHAM YOUNG UNIVERSITY
    Inventors: Paul B. Farnsworth, Sonika Sharma, H. Dennis Tolley, Milton L. Lee
  • Patent number: 10031104
    Abstract: A micro-lab includes one or more electrophoresis devices each optically coupled to respective spectrometers and electronic signal processing, analysis and control, with fluids transported via a system of valves, tubes and pumps. The spectrograms are captured by a respective digital cameras, and chemical characteristics including molecular mobility, particle (molecular) charge, molecular weight, particle (molecular) pH, particle (molecular) dielectric, particle (molecular) conductivity, Raman spectrum of each chemical species, IR spectrum of particle (molecular) is determined, and principal component analysis is performed to identify and quantify chemical constituents.
    Type: Grant
    Filed: June 12, 2015
    Date of Patent: July 24, 2018
    Assignee: YES WAY INTELLECTUAL HOLDINGS, LLC
    Inventor: Kevin M. Ferguson
  • Patent number: 10018550
    Abstract: Provided are a system and a method for analyzing a blood cell. The system includes a laser generator installed around a moving path, along which an analysis target blood cell moves, to generate a laser beam; a plurality of photodetectors configured to detect a laser beam generated as a result of refraction, reflection, transmission or fluorescence of the laser beam generated from the laser generator and incident upon the analysis target blood cell; and an optical fiber configured to focus the laser beam generated from the laser generator and to irradiate the focused laser beam to a measurement point existing on the moving path, wherein the optical fiber transfers a laser beam scattered through a blood cell to the photodetectors; and an analysis apparatus configured to analyze the analysis target blood cell by using the laser beam detected by the photodetectors.
    Type: Grant
    Filed: November 15, 2016
    Date of Patent: July 10, 2018
    Assignee: NEW OPTICS, LTD.
    Inventors: Kyu Man Lee, Tae Ho Jun
  • Patent number: 10018590
    Abstract: A method of fluid testing includes pressurizing a fluid testing system, disposed at a subterranean location under high pressure compared to a surface pressure, to achieve a desired pressure differential between the high pressure and an internal pressure of the fluid testing system. The fluid testing system includes a capillary electrophoresis system and one or more test fluid reservoirs. The method also includes directing test fluid from the one or more test fluid reservoirs into capillaries to condition the capillaries. The method further includes directing sample fluid into the capillaries for testing while at the subterranean location.
    Type: Grant
    Filed: August 15, 2013
    Date of Patent: July 10, 2018
    Assignee: SCHLUMBERGER TECHNOLOGY CORPORATION
    Inventors: Sharath Chandra Mahavadi, Geza Horvath Szabo, Simon Ivar Andersen
  • Patent number: 9989516
    Abstract: According to one aspect, the disclosure is directed to an example embodiment in which a circuit-based arrangement includes a circuit-based substrate securing a channel, with an effective width that is not limited by the Debye screening length, along a surface of the substrate. A pair of reservoirs are included in or on the substrate and configured for containing and presenting a sample having bio-molecules for delivery in the channel. A pair of electrodes electrically couple a charge in the sample to enhance ionic current flow therein (e.g., to overcome the electrolyte screening), and a sense electrode is located along the channel for sensing a characteristic of the biological sample by using the electrostatic interaction between the enhanced ionic current flow of the sample and the sense electrode. Actual detection occurs by using a charge-signal processing circuit to process the sensed charge signal and, therefrom, provide an output indicative of a signature for the bio-molecules delivered in the channel.
    Type: Grant
    Filed: September 20, 2016
    Date of Patent: June 5, 2018
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Yang Liu, Robert W. Dutton, Roger T. Howe
  • Patent number: 9977028
    Abstract: A mass spectrometry-based method for analyzing an acidic organic target compound includes directing a charged solvent (44) toward a pre-acidified sample (12) comprising the target compound, to thereby ionize the pre-acidified sample (12). The method further includes directing the ionized pre-acidified sample (54) to a mass spectrometer (18), the mass spectrometer (18) being configured to identify and quantify the target compound.
    Type: Grant
    Filed: November 7, 2013
    Date of Patent: May 22, 2018
    Assignee: Ohio University
    Inventors: Hao Chen, Ning Pan, Pengyuan Liu
  • Patent number: 9976417
    Abstract: A method improves the capability for testing a fluid sample, e.g. testing a reservoir sample of hydrocarbon fluid. The methodology comprises positioning a capillary electrophoresis system within an enclosed chamber system. The enclosed chamber system preserves the desired downhole reservoir conditions during testing of the reservoir sample. In some applications, the reservoir sample is divided into a plurality of capillaries of the capillary electrophoresis system to enable testing of the reservoir sample with different types of detectors in one capillary electrophoresis system. The method can also be applied to depressurized reservoir samples.
    Type: Grant
    Filed: June 13, 2013
    Date of Patent: May 22, 2018
    Assignee: SCHLUMBERGER TECHNOLOGY CORPORATION
    Inventors: Sharath Chandra Mahavadi, Geza Horvath Szabo, Simon Ivar Andersen
  • Patent number: 9932251
    Abstract: Disclosed are microfluidic devices and systems for the desalination of water. The devices and systems can include an electrode configured to generate an electric field gradient in proximity to an intersection formed by the divergence of two microfluidic channels from an inlet channel. Under an applied bias and in the presence of a pressure driven flow of saltwater, the electric field gradient can preferentially direct ions in saltwater into one of the diverging microfluidic channels, while desalted water flows into second diverging channel. Also provided are methods of using the devices and systems described herein to decrease the salinity of water.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: April 3, 2018
    Assignee: Board of Regents, The University of Texas System
    Inventors: Richard A. Crooks, Kyle N. Knust, Robbyn K. Perdue
  • Patent number: 9921164
    Abstract: A system and a method for optical sensing of single molecule or molecules in various concentrations are provided. The optical sensor system comprises a first fiber, a second fiber, a light source and a detection device. The first fiber and the second fiber are fused together to form an optical coupler. The first fiber serves as the passageway for the analyte, while the second fiber serves as the waveguide for the light that will interact with the said analyte. One end of the second fiber is connected to the light source (e.g. laser), and the opposite end is connected to the detection device (e.g. spectrometer). The analyte is introduced into the first fiber through one of its ends, and is allowed to flow through inside the hollow core of the said first fiber. When light is delivered through the input end of the second fiber, the evanescent light is formed in the optical coupler and is allowed to interact with the analyte in the first fiber.
    Type: Grant
    Filed: January 27, 2017
    Date of Patent: March 20, 2018
    Assignee: UVic Industry Partnerships Inc.
    Inventor: Tao Lu
  • Patent number: 9859514
    Abstract: The present disclosure relates to a method for making nanoscale heterostructure. The method includes: forming a first carbon nanotube layer on a support, the first carbon nanotube layer includes a number of first carbon nanotubes; forming a semiconductor layer on a surface of the first carbon nanotube layer; covering a second carbon nanotube layer on the semiconductor layer, the second carbon nanotube layer includes a number of second carbon nanotubes; finding and labeling a first metal carbon nanotube and a semiconductor carbon nanotube parallel to and spaced away from the first metal carbon nanotube; finding and labeling a second metal carbon nanotube, an extending direction of the second metal carbon nanotube is crossed with an extending direction of the first metal carbon nanotube and the semiconductor carbon nanotube; removing the other carbon nanotubes; and annealing the above structure.
    Type: Grant
    Filed: April 4, 2017
    Date of Patent: January 2, 2018
    Assignees: Tsinghua University, HON HAI PRECISION INDUSTRY CO., LTD.
    Inventors: Jin Zhang, Yang Wei, Kai-Li Jiang, Shou-Shan Fan
  • Patent number: 9810681
    Abstract: The invention includes compositions, devices, and methods for analyzing a polymer and/or polymer unit. The polymer may be a homo or hetero-polymer such as DNA, RNA, a polysaccharide, or a peptide. The device includes electrodes that form a tunnel gap through which the polymer can pass. The electrodes are functionalized with a reagent attached thereto, and the reagent is capable of forming a transient bond to a polymer unit. When the transient bond forms between the reagent and the unit, a detectable signal is generated and used to analyze the polymer.
    Type: Grant
    Filed: September 11, 2015
    Date of Patent: November 7, 2017
    Assignee: Arizona Board of Regents on behalf of Arizona State University
    Inventors: Stuart Lindsay, Shuai Chang, Jin He, Peiming Zhang, Shuo Huang
  • Patent number: 9766207
    Abstract: Methods and devices for purifying, detecting, and collecting analytes fractionated based on pI, separating analytes via electrophoresis and pI, and purifying a target molecule using pI focusing and subsequent crystallization are provided.
    Type: Grant
    Filed: August 26, 2014
    Date of Patent: September 19, 2017
    Assignees: Bio-Rad Laboratories, Inc., Technion Research & Development Foundation Ltd.
    Inventors: Aran Paulus, Camille Diges, Roumen Bogoev, Inbal Zafir-Lavie, Sricharan Bandhakavi, Annett Hahn-Windgassen, Anton Posch, Elad Brod, Uri Sivan
  • Patent number: 9765326
    Abstract: An apparatus for nucleic acid sequencing includes a nanochannel and a conveying device, configured to move a nucleic acid strand through the nanochannel. The conveying device includes: a first electrode, a second electrode, and a third electrode, which are arranged along the nanochannel so as to be in contact with a fluid occupying the nanochannel, the second electrode being arranged between the first electrode and the third electrode; and a control unit configured to apply a first voltage, a second voltage, and a third voltage, respectively, to the first electrode, the second electrode and the third electrode, for controlling movement of the nucleic acid strand through the nanochannel.
    Type: Grant
    Filed: April 1, 2015
    Date of Patent: September 19, 2017
    Assignee: STMicroelectronics S.r.l.
    Inventors: Francesco Ferrara, Marco Angelo Bianchessi
  • Patent number: 9757727
    Abstract: Hydrodynamic Trap Array. The array includes a serpentine bypassing channel including a plurality of trapping pockets disposed therein, the trapping pockets including a ramp entry portion to decrease flow velocity orthogonal to the trapping pocket to increase trapping efficiency. The relative fluid resistances of the trapping pockets and the serpentine bypassing channel are selected such that a slight majority of the flow is diverted to one of the trapping pockets. A pair of microfluidic bypass channels flank the array of traps allowing independent control of upstream and downstream pressures on each side of the array, thereby decoupling flow magnitude in the bypass channels from flow across the trapping pockets.
    Type: Grant
    Filed: May 18, 2015
    Date of Patent: September 12, 2017
    Assignee: Massachusetts Institute of Technology
    Inventors: Scott R. Manalis, Robert John Kimmerling, Shijie Nigel Chou, Vivian C. Hecht
  • Patent number: 9732381
    Abstract: The invention relates to a method for sequencing a heteropolymeric target nucleic acid sequence that involves stochastic sensing. The invention also relates to a method for improving a pore for sequencing a target nucleic acid sequence by modifying one or more sites in the pore.
    Type: Grant
    Filed: March 25, 2010
    Date of Patent: August 15, 2017
    Assignee: OXFORD UNIVERSITY INNOVATION LIMITED
    Inventors: David Stoddart, Andrew John Heron, Giovanni Maglia, John Hagan Pryce Bayley
  • Patent number: 9702823
    Abstract: An instrument for processing and/or measuring a biological process contains an excitation source, a sample holder, an optical sensor, an excitation optical system, and an emission optical system. The sample holder is configured to receive a plurality of biological samples. The optical sensor is configured to receive an emission from the biological samples. The excitation optical system is disposed along an excitation optical path and is configured to direct the electromagnetic radiation from the excitation source to the biological samples. The emission optical system is disposed along an emission optical path and is configured to direct electromagnetic emissions from the biological samples to the optical sensor. The instrument further contains a plurality of filter assemblies configured to be interchangeably located along at least one of the optical paths.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: July 11, 2017
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Kevin Maher, Mingsong Chen, Jacob Freudenthal, Kuan Moon Boo, Soo Yong Lau, David Fortescue, Ming Shen, Woon Liang Terence Soh
  • Patent number: 9678038
    Abstract: Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods.
    Type: Grant
    Filed: November 15, 2013
    Date of Patent: June 13, 2017
    Assignee: The Trustees of Princeton University
    Inventors: Robert H. Austin, Zhaoning Yu, Jonas O. Tegenfeldt, Stephen Y. Chou, Han Cao
  • Patent number: 9657286
    Abstract: To allow fully automated implementation of all steps of from pre-processing up to electrophoresis. Provided is a pre-processing/electrophoresis integrated cartridge including one or more block structures corresponding to the individual steps of pre-processing. Each block structure includes (1) a first block having a reaction tank, a reagent tank, a plurality of flow channels that connect the tanks, a plurality of control valves arranged in the flow channels, and a flow channel that connects a reaction tank in a block structure located at a preceding stage and a reaction tank in a block structure located at a next stage, and (2) a second block having a phoretic solution tank, a cathode buffer solution tank, a wash solution tank, a flow channel that connects a carrier tank in the block structure located at the preceding stage and the phoretic solution tank, and a control valve for the flow channel.
    Type: Grant
    Filed: June 11, 2013
    Date of Patent: May 23, 2017
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Yukari Sahoyama, Motohiro Yamazaki, Yoshitaka Kodama, Takamichi Muramatsu
  • Patent number: 9653276
    Abstract: Methods and systems for generating ions from a liquid sample for mass spectrometry are provided herein. In various aspects, the methods and systems can enhance the break-up of a jet of the liquid sample upon injection into an ionization chamber. In some aspects, methods and systems perturb the liquid sample prior to discharge to increase the internal energy of the sample so as to enhance the formation of liquid droplets when the liquid sample is injected into the ionization chamber.
    Type: Grant
    Filed: August 5, 2014
    Date of Patent: May 16, 2017
    Assignee: DH Technologies Development Pte Ltd.
    Inventors: Thomas Covey, Peter Kovarik
  • Patent number: 9612186
    Abstract: A device, a method and a system for optically determining particle properties, in particular size and reflectivity. The device includes at least one light source assembly having at least one light source, a polarizer assembly, at least one sample holder—which can be illuminated by the least one light source assembly—for accommodating particle preparations to be investigated, at least one analyzer assembly, and at least one imaging device with at least one color-resolving matrix image sensor. The device is designed to guide light reflected by a particle preparation and having a color-coded polarization to the at least one matrix image sensor.
    Type: Grant
    Filed: March 24, 2016
    Date of Patent: April 4, 2017
    Assignee: OLYMPUS SOFT IMAGING SOLUTIONS GMBH
    Inventor: Yi Wei
  • Patent number: 9611199
    Abstract: A method includes calibrating color bleed factors of optical detector channels of a sample processing apparatus through processing a color bleed calibration substance which includes a plurality of different size fragments replicated from different groups of DNA loci, wherein fragments in a same group are labeled with a same fluorescent dye, and fragments in different groups are labeled with different fluorescent dyes having different emission spectra, wherein the different size fragments are processed during different acquisition times.
    Type: Grant
    Filed: September 28, 2015
    Date of Patent: April 4, 2017
    Assignee: Analogic Corporation
    Inventor: Ching Ming Lai
  • Patent number: 9606083
    Abstract: The invention provides methods and systems for ruggedizing a nucleic acid analyzing apparatus. The ruggedized apparatus can be used reliably and effectively in uncontrolled environments, such as, for example at a crime scene to collect and analyze forensic data, as well as in semi-controlled environments, such as, for example at a point of care location.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: March 28, 2017
    Assignee: NETBIO, INC.
    Inventors: Eugene Tan, Heung Chuan Lam, Gregory John Kellogg
  • Patent number: 9605300
    Abstract: A technique for performing spectral calibration simultaneously with electrophoresis of an actual sample to be analyzed, without performing electrophoresis using a special matrix standard which is time-consuming and costly, is provided. The device for genotypic analysis is characterized by obtaining reference fluorescence spectra using a size standard and an allelic ladder, which provide information concerning known DNA fragments used for electrophoresis of an actual sample, and is characterized by performing spectral calibration for a capillary in which the allelic ladder is not used by detecting a shift amount of the fluorescence spectra of the size standard and shifting the reference fluorescence spectra using the shift amount to determine fluorescence spectra.
    Type: Grant
    Filed: December 4, 2013
    Date of Patent: March 28, 2017
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Toru Yokoyama, Motohiro Yamazaki
  • Patent number: 9593372
    Abstract: The present invention is directed to systems, devices and methods for identifying biopolymers, such as strands of DNA, as they pass through a constriction such as a carbon nanotube nanopore. More particularly, the invention is directed to such systems, devices and methods in which a newly translocated portion of the biopolymer forms a temporary electrical circuit between the nanotube nanopore and a second electrode, which may also be a nanotube. Further, the invention is directed to such systems, devices and methods in which the constriction is provided with a functionalized unit which, together with a newly translocated portion of the biopolymer, forms a temporary electrical circuit that can be used to characterize that portion of the biopolymer.
    Type: Grant
    Filed: December 19, 2014
    Date of Patent: March 14, 2017
    Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
    Inventors: Stuart Lindsay, Peiming Zhang
  • Patent number: 9581536
    Abstract: A lab on a chip device includes a whole blood inlet port and microchannels to transport a whole blood sample or plasma skimmed from the whole blood sample into a detection chamber that includes at least one 3-electrode set of a counter electrode, a working electrode and a reference electrode. The counter electrode, the working electrode and the reference electrode may present bare, unmodified surfaces that are disposed so that clozapine present in the whole blood sample is detected via a reduction-oxidation reaction. Alternatively, the working electrode surface may include catechol grafted to chitosan. A method of detecting analytes and biomarkers includes collecting a whole blood sample, loading the sample into a point-of-care testing (POCT) device that includes at least one working electrode; testing the sample for the occurrence of a redox reaction; and calculating the total oxidative charge when the working electrode is bare or modified as before.
    Type: Grant
    Filed: May 9, 2014
    Date of Patent: February 28, 2017
    Assignees: UNIVERSITY OF MARYLAND, UNIVERSITY OF MARYLAND
    Inventors: Hadar Ben-Yoav, Reza Ghodssi, Gregory F Payne, Deanna L Kelly, Eunkyoung Kim, Thomas E Winkler
  • Patent number: 9575058
    Abstract: The present invention provides fluidic devices and systems that allow detection of analytes from a biological fluid. The methods and devices are particularly useful for providing point-of-care testing for a variety of medical applications.
    Type: Grant
    Filed: November 5, 2014
    Date of Patent: February 21, 2017
    Assignee: Theranos, Inc.
    Inventors: Shaunak Roy, Malissa Takahashi, Ian Gibbons, Edmond Ku, Thanh Dang, Tammy Burd, Adam Vollmer, Elizabeth A. Holmes
  • Patent number: 9568575
    Abstract: This invention features devices and methods for analyte processing and detection, and use of such methods, e.g., in the treatment and diagnosis of disease or determining the presence of a pathogen.
    Type: Grant
    Filed: October 19, 2011
    Date of Patent: February 14, 2017
    Assignee: T2 Biosystems, Inc.
    Inventors: Thomas Jay Lowery, Jr., Parris S. Wellman, John J. McDonough, James Franklin Chepin, Charles William Rittershaus, Vasiliki Demas, Alison Skelley
  • Patent number: 9518955
    Abstract: The invention is a high sensitivity and high throughput capillary multiwavelength fluorescence detection system. The fluorescent detection system is configured to illuminate a relatively large volume of a single capillary or a plurality of capillaries, with a pixelated detection system capable of imaging an area of each capillary that differentiates the capillary walls, the space between the capillaries, and the internal liquid volume within the capillary. Only the desired pixels or image area are used for processing and generating an output signal.
    Type: Grant
    Filed: December 9, 2009
    Date of Patent: December 13, 2016
    Assignee: ADVANCED ANALYTICAL TECHNOLOGIES, INC.
    Inventors: Pang Ho-ming, Wei Wei
  • Patent number: 9500588
    Abstract: A liquid sample analyzer includes a liquid sample source, a flow cell, an optical device and a plurality of optical fibers. The flow cell is configured to receive a flow of a liquid sample from the liquid sample source. The plurality of optical fibers optically connect the flow cell to the optical device to transmit light between the flow cell and the optical device.
    Type: Grant
    Filed: September 30, 2014
    Date of Patent: November 22, 2016
    Assignee: PerkinElmer Health Sciences, Inc.
    Inventors: Joseph L. DiCesare, Feng Jin, Timothy P. Neal, David Aikens