Capillary Electrophoresis Patents (Class 204/451)
  • Patent number: 11130997
    Abstract: The present invention provides methods for analyzing large nucleic acids including chromosomes and chromosomal fragments. In one aspect, the present invention provides a method of nucleic acid analysis comprising the steps of (a) obtaining a sample of nucleic acid comprising at least one chromosome or fragment greater than about 1 000 base pairs in length and containing a target region; (b) creating an emulsion in which each drop of the emulsion contains an average of between about 0-2, 0-1.75, 0-1.5, 0-1.0, 0-0.75, 0-0.5, or fewer chromosomes or fragments of step (a), (c) performing emulsion PCR, (d) quantifying the number of emulsion droplets containing amplified nucleic acid from the target region; (e) calculating the ratio of droplets containing amplified nucleic acid from the target region to total droplets; and (f) comparing the ratio of step (e) to a reference ratio representing a known genotype.
    Type: Grant
    Filed: March 10, 2017
    Date of Patent: September 28, 2021
    Assignee: Quest Diagnostics Investments Incorporated
    Inventor: Charles M. Strom
  • Patent number: 11097271
    Abstract: A microfluidic device can comprise a plurality of interconnected microfluidic elements. A plurality of actuators can be positioned abutting, immediately adjacent to, and/or attached to deformable surfaces of the microfluidic elements. The actuators can be selectively actuated and de-actuated to create directed flows of a fluidic medium in the microfluidic (or nanofluidic) device. Further, the actuators can be selectively actuated and de-actuated to create localized flows of a fluidic medium in the microfluidic device to move reagents and/or micro-objects in the microfluidic device.
    Type: Grant
    Filed: August 7, 2018
    Date of Patent: August 24, 2021
    Assignee: Berkeley Lights, Inc
    Inventors: Keith J. Breinlinger, Andrew W. McFarland, J. Tanner Nevill
  • Patent number: 11085897
    Abstract: It is an object to repeatedly use a microchip without complicating the structure of the microchip, as well as not impairing cost and operability. A seal-attached member 26 is disposed facing a microchip 5 held by a chip holding unit 7, and has through holes 64-3 and 64-4 provided at positions corresponding to reservoirs 53-3 and 53-4, respectively, and elastic members 67 that are pressed against the microchip 5 so as to maintain airtightness between the through holes 64-3 and 64-4 and the corresponding reservoirs 53-3 and 53-4. When the inside of a flow path 55 of the microchip 5 is cleaned, a dispensing probe 8 is inserted into the through hole 64-4 while airtightness between the through hole 64-4 and the dispensing probe 8 is maintained. Then, a suction nozzle 22-3 is inserted into the through hole 64-3.
    Type: Grant
    Filed: September 13, 2018
    Date of Patent: August 10, 2021
    Assignee: SHIMADZU CORPORATION
    Inventor: Akihiro Arai
  • Patent number: 11067536
    Abstract: The invention is an improved multiplex capillary electrophoresis instrument or module with at least four and preferably six user-accessible vertically stacked drawers. An x-z stage moves samples from the user accessible drawers to the capillary array for analysis. A computer program allows users to add capillary electrophoresis jobs to a queue corresponding to the analysis of rows or plates of samples without stopping or interrupting runs in progress.
    Type: Grant
    Filed: June 13, 2018
    Date of Patent: July 20, 2021
    Assignee: Agilent Technologies, Inc.
    Inventors: Bruce R. Boeke, Martin C. Foster, Thomas J. Kurt, Scott Stueckradt
  • Patent number: 11053535
    Abstract: Devices, such as chips for DNA analysis, have at least one fluid transport nanochannel with at least one intersecting (e.g., transverse) sensing nanochannel that can be monitored for change in ionic current to determine characteristics or parameters of interest, e.g., molecular identification, length determination, localized (probe) mapping and the like.
    Type: Grant
    Filed: September 7, 2012
    Date of Patent: July 6, 2021
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: John Michael Ramsey, Jean Pierre Alarie, Laurent Menard
  • Patent number: 11034853
    Abstract: The invention is directed to methods of modifying metal oxide or hydroxylated polymer surfaces using compositions of water soluble polymers that adsorb onto such surfaces and that contain functional groups which directly modify such surfaces without further processing. In some embodiments, compositions used in such methods include water-soluble oxide-adsorbing polymers having water solubility in an indicated temperature range, an indicated concentration in the aqueous solution, and a molecular weight range, wherein each of the water-soluble oxide-adsorbing polymers comprises a linear copolymer comprising a first monomer having at least one hydrophilic moiety and a second monomer having at least one lipophilic moiety and wherein a first monomer: second monomer molecular ratio is at least 3:1.
    Type: Grant
    Filed: January 30, 2018
    Date of Patent: June 15, 2021
    Assignee: Quantapore, Inc.
    Inventor: Steven Menchen
  • Patent number: 11035824
    Abstract: The invention relates to a closure for an electrochemical reaction vessel, in particular a potentiostat, the closure comprising: a holder for holding electrodes arranged at an inner side of the closure such that, when the closure is attached to a reaction vessel, electrodes held by the holder extend into an interior space of the reaction vessel and into an electrolyte contained in the reaction vessel; and a plurality of contacts arranged at an outer side of the closure for providing electrical contacts with the electrodes.
    Type: Grant
    Filed: April 20, 2020
    Date of Patent: June 15, 2021
    Assignee: IKA-Werke GmbH & CO. KG
    Inventors: Phil S. Baran, Evan Horn, Dirk Waldmann
  • Patent number: 11029315
    Abstract: Flow apparatuses comprising a separation channel, a downstream flow separator, a detection zone, an observation zone, and a waste channel. The separation channel has first and second flows in contact and allows lateral movement of components between contacting first and second flows. The downstream flow separator is in communication with the separation channel and diverts a part of the first fluid flow, the second fluid flow, or both, from the separation channel. The detection zone comprises a detection channel downstream of and in communication with the flow separator and configured to receive a plurality of diverted flows from the flow separator and a label channel configured to label the diverted flows from the flow separator. The observation zone is configured to record an analytical signal indicative of the quantity and the electrical properties of the component. The waste channel is at the downstream end of the observation zone.
    Type: Grant
    Filed: April 8, 2019
    Date of Patent: June 8, 2021
    Assignee: Cambridge Enterprise Limited
    Inventors: Emma Yates, Christopher Dobson, Therese Herling, Tuomas Pertti Jonathan Knowles
  • Patent number: 10994274
    Abstract: An EWOD device includes a first substrate assembly and a second substrate assembly; wherein one of said substrate assemblies includes electrowetting electrodes, and the first substrate assembly and the second substrate assembly are spaced apart to define a channel between the substrate assemblies; and a housing for receiving the first substrate assembly and the second substrate assembly, the housing comprising an alignment feature for locating at least one of the first and second substrate assemblies within the housing. The device further includes a fixing feature for fixing the first and second substrate assemblies within the housing. The second substrate assembly is located within the housing such that the second substrate assembly is an outer component of the EWOD device. The device further may include a spacer that spaces apart the first substrate assembly from the second substrate assembly to define the channel between the first and second substrate assemblies.
    Type: Grant
    Filed: July 12, 2017
    Date of Patent: May 4, 2021
    Assignee: Sharp Life Science (EU) Limited
    Inventors: Lesley Anne Parry-Jones, Emma Jayne Walton
  • Patent number: 10989705
    Abstract: A micro-electromechanical platform and array system and methods for identifying microbial species with single molecule electrical conductance measurements are provided. The electromechanical platform has a two-tier actuation mechanism with a long stroke provided by a comb drive and a fine stroke provided by an in-plane flexural actuator. The platform is capable of making contact with a single-molecule, applying a bias, measuring the current, and performing a large number of measurements for statistical analysis. The system is capable of detecting any microbial species without requiring enzymatic amplification by detecting specific RNA sequences, for example. With oligonucleotide target molecules, the conductance is extremely sensitive to the sequence so even single-nucleotide polymorphisms can be identified. The system can also discern between subspecies using the same DNA probe.
    Type: Grant
    Filed: July 11, 2017
    Date of Patent: April 27, 2021
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Joshua Hihath, Xiaoguang Liu, Maria Louise Marco
  • Patent number: 10948454
    Abstract: Methods for nanopore-based protein analysis are provided. The methods address the characterization of a target protein analyte, which has a dimension greater than an internal diameter of the nanopore tunnel, and which is also physically associated with a polymer. The methods further comprise applying an electrical potential to the nanopore system to cause the polymer to interact with the nanopore tunnel. The ion current through the nanopore is measured to provide a current pattern reflective of the structure of the portion of the polymer interacting with the nanopore tunnel. This is used as a metric for characterizing the associated protein that does not pass through the nanopore.
    Type: Grant
    Filed: July 22, 2019
    Date of Patent: March 16, 2021
    Assignee: University of Washington
    Inventors: Jens Gundlach, Ian Michael Derrington, Andrew Laszlo, Jonathan Craig, Henry Brinkerhoff
  • Patent number: 10908143
    Abstract: Conventionally, only a pair of electrodes is provided and nanopores arranged in parallel are connected by an electrolyte solution, and therefore a change in an ion current to be measured is a sum of changes in ion currents generated in the respective nanopores.
    Type: Grant
    Filed: November 27, 2013
    Date of Patent: February 2, 2021
    Assignee: Hitachi, Ltd.
    Inventors: Takahide Yokoi, Takashi Anazawa
  • Patent number: 10898871
    Abstract: A MEMS multiplexing system including: first and second fluid inputs; and a mixing network. The mixing network including: a first channel to receive the first fluid input; a second channel to receive the second fluid input; a multiplexing valve communicating with the first channel and the second channel, the multiplexing valve to cause the transport of the first fluid into the second channel so as to form a first interleaved fluid downstream from the multiplexing valve in the second channel and to cause the transport of the second fluid into the first channel so as to form a second interleaved fluid downstream from the multiplexing valve in the first channel; and the first channel and the second channel intersecting downstream from the valve so as to force mixing of the first interleaved fluid and the second interleaved fluid.
    Type: Grant
    Filed: July 2, 2018
    Date of Patent: January 26, 2021
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Jonathan Fry, Daniel Piper, Jang Sim, Yongchun Xin
  • Patent number: 10870823
    Abstract: Described herein are apparatus comprising: a first layer comprising a first microfluidic channel; a second layer comprising a second microfluidic channel; and a membrane for culturing cells; along with methods of making and using same.
    Type: Grant
    Filed: June 12, 2015
    Date of Patent: December 22, 2020
    Assignee: LEHIGH UNIVERSITY
    Inventors: Yaling Liu, Antony Thomas
  • Patent number: 10857536
    Abstract: Examples include polymerase chain reaction (PCR) devices. Example PCR devices comprise a fluid input, a fluid output, and a set of microfluidic channels that fluidly connect the fluid input and the fluid output. Each microfluidic channel comprises a reaction chamber, and examples further comprise at least one heating element, where the at least one heating element is positioned in the reaction chamber of each microfluidic channel. The at least one heating element is to heat fluid in the reaction chamber of each fluid channel, and the at least one heating element is to pump fluid to the reaction chamber and from the reaction chamber of each microfluidic channel.
    Type: Grant
    Filed: January 8, 2016
    Date of Patent: December 8, 2020
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Erik D Torniainen, Alexander Govyadinov, Pavel Kornilovich, David P Markel
  • Patent number: 10851335
    Abstract: In one embodiment, a flow assembly for cells comprises a first flow path configured to receive a plurality of cells, a second flow path configured to receive a buffer, and a third flow path configured to receive the plurality of cells and the buffer. The plurality of cells are in a single-file orientation and the buffer generally surrounds the single-file orientation of the plurality of cells when in the third flow path.
    Type: Grant
    Filed: March 31, 2016
    Date of Patent: December 1, 2020
    Assignee: Indiana University Research and Technology Corporation
    Inventors: Lisa M. Jones, Aimee Rinas
  • Patent number: 10851405
    Abstract: Methods and related products are disclosed that improve the probability of interaction between a target molecule and a nanopore by capturing the target molecule on a surface comprising the nanopore. The captured target molecule, the nanopore, or both, are able to move relative to each other along the surface. When the leader of the target molecule is in proximity with the nanopore, interaction of the target portion of the target molecule with the nanopore occurs, thereby permitting sensing of the target portion. Confining the target molecule and nanopore in this manner leads to significantly enhanced interaction with the nanopore.
    Type: Grant
    Filed: May 2, 2017
    Date of Patent: December 1, 2020
    Assignee: STRATOS GENOMICS, INC.
    Inventors: Mark Stamatios Kokoris, Robert N. McRuer
  • Patent number: 10828639
    Abstract: A fluid exit passage is at a location along a microfluidic channel. A fluid displacement device is proximate the location along the microfluidic channel. A constituent locator distinguishes a target constituent in a fluid within the microfluidic channel from remaining non-target constituents and locate the target constituent proximate the fluid exit passage. A controller selectively actuates the fluid displacement device when the target constituent is proximate the fluid exit passage to discharge the target constituent from the microfluidic channel through the fluid exit passage.
    Type: Grant
    Filed: April 30, 2015
    Date of Patent: November 10, 2020
    Assignee: Hewlett-Packard Development Company L.P.
    Inventors: Anita Rogacs, Alexander Govyadinov
  • Patent number: 10768150
    Abstract: The present disclosure provides a method for conducting comprehensive chromatography analysis. Broadly, the method comprises separating a sample in a first chromatographic column to generate a primary stream, which is directed toward a non-modulator switching system comprising at least one micro-switch and at least one valve. The non-modulator switching system is continuously operated to: (a) selectively direct a portion of the primary stream to one of a plurality of thermal injectors and accumulating the portion of the primary stream for a predetermined amount of time; (b) inject the portion of the primary stream into one of a plurality of secondary chromatographic columns; (c) detect one or more analytes in a secondary stream exiting the secondary chromatographic column; and repeat (a)-(c) to selectively direct other portions of the primary stream to other thermal injectors and secondary chromatographic columns until all of the analytes in the sample are detected.
    Type: Grant
    Filed: October 4, 2017
    Date of Patent: September 8, 2020
    Assignee: THE REGENTS OF THE UNIVERSITY OF MICHIGAN
    Inventors: Xudong Fan, Jiwon Lee, Menglian Zhou, Hongbo Zhu, Katsuo Kurabayashi
  • Patent number: 10750928
    Abstract: Devices and methods for preparing RNA and DNA from single cells are disclosed. In particular, the invention relates to a method of simultaneously extracting RNA and DNA from single cells and separating the nucleic acids electrophoretically. An electric field is used to lyse a single target cell, such that the plasma membrane is selectively disrupted without lysing the nuclear membrane. Cytoplasmic RNA is separated from the nucleus by performing isotachophoresis (ITP) in the presence of a sieving matrix that preferentially reduces the mobility of the nucleus. During ITP, the cytoplasmic RNA accumulates at an ITP interface between leading and trailing electrolytes and can later be extracted free of nuclear DNA. The method can be performed in a microfluidic device that fully automates all steps of the process.
    Type: Grant
    Filed: January 9, 2015
    Date of Patent: August 25, 2020
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Juan G Santiago, Hirofumi Shintaku
  • Patent number: 10716887
    Abstract: Provided is a filtration module for separating plasma from blood comprising a feeder channel lid, a feeder channel defined by a feeder channel laminating layer having a thickness of less than 5 mil, a filter element in fluid communication with the feeder channel and having a pore size of less than 2 microns and low surface area, and a filtrate take-off port having a dead volume of less than 10 ?L. Also provided are methods for filtering a blood sample comprising supplying the blood sample to a feeder channel of a filtration module and drawing the blood sample over a filter element of the filtration module in a single pass process configuration to provide a retentate and a plasma filtrate.
    Type: Grant
    Filed: July 1, 2018
    Date of Patent: July 21, 2020
    Assignee: PDL BioPharma, Inc.
    Inventor: Jonathan Leland
  • Patent number: 10712354
    Abstract: There is provided a method of analyzing a biological sample component that allows easy and accurate quantification and counting of any of a plasma component and a blood cell component in a trace and unknown amount of a whole blood sample collected from a finger, for example. The method of the present invention is a method of analyzing a biological sample component in a trace amount of blood, comprising analyzing a diluent buffer into which the blood has been mixed and an internal standard substance and/or an external standard substance contained in the diluent buffer, calculating a dilution ratio, and analyzing a biological component in a plasma or serum component in the blood.
    Type: Grant
    Filed: July 6, 2015
    Date of Patent: July 14, 2020
    Assignee: Leisure, Inc.
    Inventors: Susumu Osawa, Shinya Sugimoto, Isao Yonekubo
  • Patent number: 10684253
    Abstract: The present invention relates to methods for the identification of compounds in carbohydrate mixture compositions as well as the determination of carbohydrate mixture composition patterns, based on e.g. orthogonal cross determining migration time (indices) using capillary gel electrophoresis-laser induced fluorescence and identifying said carbohydrate components based on comparing said migration time (indices) with standard migration time (indices) from a database which data are preferably also orthogonal cross determined. In a further aspect, the present invention relates to a method for carbohydrate mixture composition pattern profiling, like glycosylation pattern profiling using capillary gel electrophoresis-laser induced fluorescence (CGE-LIF). In another aspect, the present invention refers to a system for an automated determination and/or identification of carbohydrates and/or carbohydrate mixture composition patterns (e.g.: glycosylation patterns).
    Type: Grant
    Filed: September 20, 2012
    Date of Patent: June 16, 2020
    Assignee: Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V.
    Inventors: Erdmann Rapp, Jana Schwarzer, Udo Reichl, Christian Bohne
  • Patent number: 10676679
    Abstract: A device includes a channel having an inlet and an outlet, a first electrode disposed within the channel, and a second electrode disposed within the channel so as to define a gap between the second electrode and the first electrode. The device further includes a power source connected to at least one of the electrodes. The second electrode includes a lumen from a first end of the second electrode to a second end of the second electrode. The lumen is configured to introduce a carrier gas to the gap. The inlet is configured to introduce a process stream to the channel. The process stream comprises a viscous petroleum feed material. The power source is configured to produce a spark within the gap, thereby generating a plasma configured to reduce a viscosity of the viscous petroleum feed material and to form a processed petroleum material.
    Type: Grant
    Filed: March 30, 2017
    Date of Patent: June 9, 2020
    Assignee: LTEOIL LLC
    Inventor: Yury Novoselov
  • Patent number: 10639630
    Abstract: A device includes a microfluidic channel structure on a substrate and a first resistive structure on the substrate to control the temperature of at least the substrate. The first resistive structure is separate from, and independent of the, microfluidic channel structure. In some instances, the device includes a second resistive structure.
    Type: Grant
    Filed: January 30, 2015
    Date of Patent: May 5, 2020
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Chantelle Domingue, Manish Giri, Sadiq Bengali
  • Patent number: 10620157
    Abstract: The present invention relates to a single point detection type microfluidic chip isoelectric focusing. The single point detection type microfluidic chip isoelectric focusing uses a microfluidic chip including first and second electrode portions storing each electrode solution at both ends and a microfluidic channel between the first and second electrode portions and includes a focusing step of respectively connecting each electrode to the first electrode portion and the second electrode portion and applying an electric field thereto to separate a biomolecule to an isoelectric point, a mobilization step of moving the focused biomolecule toward a detection point by removing the electrode solution in the first electrode portion or the second electrode portion, and a detection step of the biomolecule moved toward the detection point.
    Type: Grant
    Filed: October 11, 2017
    Date of Patent: April 14, 2020
    Assignee: MYONGJI UNIVERSITY INDUSTRY AND ACADEMIA COOPERATION FOUNDATION
    Inventors: Dohyun Kim, Jin Song, Nebiyu Getachew Arega
  • Patent number: 10598631
    Abstract: Method for electrophoretic separation using a separation gel arrangement with a gel member and one or more sample wells for receiving sample liquid to be separated, the sample wells being in fluidic contact with the gel member, the method comprising the steps: adding a sample liquid to be separated in one or more of the sample wells, applying an electric field over the gel member to drive an electrophoretic separation process, whereby sample constituents are drawn from the sample liquid in the sample well(s) into the gel member for separation, and when a removal criteria is met: discontinuing loading of sample constituents into the separation gel.
    Type: Grant
    Filed: March 23, 2015
    Date of Patent: March 24, 2020
    Assignee: GE Healthcare Bio-Sciences AB
    Inventor: Kjell Larsson
  • Patent number: 10597702
    Abstract: Provided are polymeric scaffold compositions and methods for detecting or quantitating diols such as carbohydrates or carbohydrate containing molecules (e.g., glycosylated protein). Provided herein are capture probes configured to bind to a scaffold. Also provided herein are capture probes linked to one or more reactive organoboronic moiety for binding diol-containing compounds in a solution. Methods of detecting complexes comprising diol-containing compounds for detecting or quantifying the presence of diol-containing compounds in solution using a nanopore device are also provided herein.
    Type: Grant
    Filed: August 26, 2014
    Date of Patent: March 24, 2020
    Assignee: Ontera Inc.
    Inventor: Trevor J. Morin
  • Patent number: 10598673
    Abstract: The present invention relates to a container comprising haemoglobin fractions, wherein said container comprising at least two compartments, wherein a first compartment comprises O2Hb (oxyhaemoglobin) and a second compartment comprises MetHb (methaemoglobin), optionally wherein O2Hb is stabilized. The invention also relates to a kit for determining the reliability of a CO-oximetry device, wherein said kit comprises said container and to a method for determining the reliability of a CO-oximetry device using said container.
    Type: Grant
    Filed: December 10, 2015
    Date of Patent: March 24, 2020
    Assignee: EUROTROL B.V.
    Inventors: Bartholomeus Henricus Antonius Maas, Carolina Johanna Huizing
  • Patent number: 10564122
    Abstract: An electrophoresis chip-based setup for detection of different ions in buffer solution. The device is able to differentiate ions in, e.g., real soil or other solutions, and to detect concentration of a specific ion in the solution. Fabrication of the electrophoresis chip can use a soft lithography based molding process. The chip can be made out of PDMS on a glass substrate where on-chip valves were used to control timing of injecting sample and buffer solutions. Detection electrodes are used to detect the presence of ions over a period of several minutes. A controllable high voltage power supply system and related signal acquisition, processing and detection setup can be implemented with the sensor in a system. A microfluidic system for automated collection of soil sample through a porous ceramic and using vacuum suction can be used.
    Type: Grant
    Filed: October 20, 2017
    Date of Patent: February 18, 2020
    Assignee: Iowa State University Research Foundation, Inc.
    Inventors: Zhen Xu, Liang Dong, Ratnesh Kumar
  • Patent number: 10562028
    Abstract: The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid.
    Type: Grant
    Filed: January 3, 2018
    Date of Patent: February 18, 2020
    Assignee: The University of Chicago
    Inventors: Rustem F. Ismagilov, Joshua David Tice, Helen Song Baca, Lewis Spencer Roach
  • Patent number: 10551358
    Abstract: This disclosure provides a single reactor that accommodates an affinity selector to separate analytes of interest, and an enzyme reactor that digest the analyte to suitable peptides for mass spectrometry. The single reactor formats described herein accommodate workflows wherein separation precedes digestion as well as workflows wherein digestion precedes separation selection.
    Type: Grant
    Filed: December 1, 2014
    Date of Patent: February 4, 2020
    Assignee: Perfinity Bioscices, Inc.
    Inventors: Kevin Wayne Meyer, John Patrick O'Grady, Bob Harold Ellis, Derrick Nathaniel Poe, Nicholas Brian Herold
  • Patent number: 10488322
    Abstract: A biological particle capturing and retrieving system includes a capturing device including a substrate, an isolating layer and a driving unit, and a retrieving device including a micropipette. The isolating layer includes a top surface, multiple pores, and multiple fluidic grooves indented from the top surface, arranged in a herringbone pattern, and each having a bottom surface. The pores are formed in the bottom surfaces of the fluidic grooves, and each capture a biological particle in a liquid sample. The driving unit drives the liquid sample to flow by electrowetting through the pores. The micropipette has a carrier coated with a biological particle-binding material binding with the biological particle received in a corresponding pore.
    Type: Grant
    Filed: March 24, 2017
    Date of Patent: November 26, 2019
    Assignee: CE BIOTECHNOLOGY, INC.
    Inventors: Chung-Er Huang, Sheng-Wen Chen, Hsin-Cheng Ho, Ming Chen
  • Patent number: 10401324
    Abstract: Devices and methods for characterization of analyte mixtures are provided. Some methods described herein include performing enrichment steps on a device before expelling enriched analyte fractions from the device for subsequent analysis. Also included are devices for performing these enrichment steps.
    Type: Grant
    Filed: December 18, 2018
    Date of Patent: September 3, 2019
    Assignee: INTABIO, INC.
    Inventor: Erik Gentalen
  • Patent number: 10393699
    Abstract: The present invention relates to a microfluidic analysis device (1) including: a substrate (20) wherein a separation channel (10) is arranged, in which an electrolyte flows, a portion of the separation channel (10) being covered with a polarizable surface (11); two longitudinal field electrodes (8a, 8b) arranged on either side of the separation channel (10); at least one control electrode (6a, 6b) positioned in the separation channel (10), the control electrode (6a, 6b) being suitable for polarizing the polarizable surface (11) so as to control the speed of the electro-osmotic flow in the separation channel (10); the microfluidic analysis device (1) being characterised in that the polarizable surface (11) includes an insulating sub-layer (12) made of amorphous silicon carbide (SiC) and an upper polarizable layer (13) in direct contact with the electrolyte, the control electrodes (6a, 6b) being positioned between the insulating sub-layer (12) and the upper polarizable layer (13).
    Type: Grant
    Filed: September 4, 2015
    Date of Patent: August 27, 2019
    Assignees: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS), UNIVERSITE PARIS—SUD
    Inventors: Anne-Marie Haghiri-Gosnet, Antoine Pallandre, Jean Gamby, Daniel Rose, Sebastien Meance
  • Patent number: 10376890
    Abstract: The disclosed apparatus, systems and methods relate to a modular microfluidic device, the component comprising a channel comprising an apex opening, wherein the apex opening that is at least partially surrounded by a collar configured to pin a liquid within the channel. The modular microfluidic device may also have a structural tip in or near the apex opening which is configured to allow for the flow of the liquid into the channel from a second component for a modular microfluidic device when the component is engaged with the second component.
    Type: Grant
    Filed: February 6, 2017
    Date of Patent: August 13, 2019
    Assignee: Stacks to the Future LLC
    Inventors: Erwin Berthier, Theodorus de Groot, Jiaquan Yu, Ashleigh Theberge, David Beebe
  • Patent number: 10357770
    Abstract: A microfluidic probe is disclosed. The microfluidic probe includes a probe head, a liquid spacer supply and a spacer modulation unit. The probe head may include a processing liquid channel in fluid communication with a processing liquid aperture provided on a face of the probe head. The probe head is configured to transport, circulate, recirculate, or move some processing liquid in (or via) the processing liquid channel toward and/or from the processing liquid aperture. The spacer supply is fluidly connected, via a spacer insertion junction, to the processing liquid channel. The spacer supply is configured for inserting liquid spacers into the processing liquid channel, via the spacer insertion junction. Liquid volumes can be obtained, which are separated by inserted liquid spacers. The spacer modulation unit is configured to control the spacer supply, to modulate the insertion of spacers via the spacer supply. Related devices and methods of operation are disclosed.
    Type: Grant
    Filed: October 9, 2015
    Date of Patent: July 23, 2019
    Assignee: International Business Machines Corporation
    Inventors: Julien Autebert, Julien Cors, Emmanuel Delamarche, Govind Kaigala, Xander Frank Van Kooten
  • Patent number: 10351901
    Abstract: A microfluidic processing device includes a substrate defining a microfluidic network. The substrate is in thermal communication with a plurality of N independently controllable components and a plurality of input output contacts for connecting the substrate to an external controller. Each component has at least two terminals. Each terminal is in electrical communication with at least one contact. The number of contacts required to independently control the N components is substantially less than the total number of terminals. Upon actuation, the components typically heat a portion of the microfluidic network and/or sense a temperature thereof.
    Type: Grant
    Filed: June 12, 2017
    Date of Patent: July 16, 2019
    Assignee: HandyLab, Inc.
    Inventors: Karthik Ganesan, Kalyan Handique
  • Patent number: 10295545
    Abstract: Flow apparatuses comprising a separation channel, a downstream flow separator, a detection zone, an observation zone, and a waste channel. The separation channel has first and second flows in contact and allows lateral movement of components between contacting first and second flows. The downstream flow separator is in communication with the separation channel and diverts a part of the first fluid flow, the second fluid flow, or both, from the separation channel. The detection zone comprises a detection channel downstream of and in communication with the flow separator and configured to receive a plurality of diverted flows from the flow separator and a label channel configured to label the diverted flows from the flow separator. The observation zone is configured to record an analytical signal indicative of the quantity and the electrical properties of the component. The waste channel is at the downstream end of the observation zone.
    Type: Grant
    Filed: March 15, 2018
    Date of Patent: May 21, 2019
    Assignee: Cambridge Enterprise Limited
    Inventors: Emma Yates, Christopher Dobson, Therese Herling, Tuomas Knowles
  • Patent number: 10274460
    Abstract: The present disclosure relates, in some embodiments, to an apparatus for conducting a capillary electrophoresis assay. The apparatus can comprise a capillary array comprising an anode end and a cathode end, the capillary array provided in a housing further comprising a reservoir configured to house a separation medium and an anode buffer. The system can also comprise an injection mechanism configured to deliver sample to the cathode end of the capillary array, and a temperature control zone, wherein the temperature control zone is configured to control the temperature of the interior of the housing.
    Type: Grant
    Filed: March 6, 2015
    Date of Patent: April 30, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Ian Walton, Achim Karger, Alexander Khorlin, Michael Simon, Adam Sannicandro, Alexander Dukhovny, Robert Cobene, Dan Kline, John Dixon
  • Patent number: 10258982
    Abstract: A combined-blade open flow path device is a fluid flow path device where flow paths are adjacent to each other. The combined-blade open flow path device comprises a substrate configured to constitute a bottom portion of the flow paths; and blades erected on a surface of the substrate, the blades being configured to constitute side walls of the flow paths, wherein the blades are erected in groups, each of the groups extending from an upstream side to a downstream side in a flow direction of a fluid with a space between each of the blades in the flow direction of the fluid in each of the groups for making conduction of the fluid between the adjacent flow paths possible, and wherein one end of one of the flow paths is configured to be in contact with the fluid and is configured to make a flow of the fluid possible.
    Type: Grant
    Filed: April 22, 2015
    Date of Patent: April 16, 2019
    Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Takahiko Hariyama, Daisuke Ishii, Masatsugu Shimomura, Ko Okumura, Marie Tani
  • Patent number: 10247710
    Abstract: Certain embodiments described herein are directed to chromatography systems that include a microfluidic device and that implement one or more methods to direct sample to a desired fluid flow path. The methods can be used to backflush a sample to a desired fluid flow path to select certain analytes within a sample.
    Type: Grant
    Filed: October 21, 2013
    Date of Patent: April 2, 2019
    Assignee: PerkinElmer Health Sciences, Inc.
    Inventor: Andrew Tipler
  • Patent number: 10208289
    Abstract: A fiber includes one or more layers of polymer surrounding a central lumen, and living animal cells disposed within the lumen and/or within at least one of the one or more layers, wherein the fiber has an outer diameter of between 5 and 8000 microns and wherein each individual layer of polymer has a thickness of between 0.1 and 250 microns. Also disclosed are model tissues including such fibers, and method of making such fibers. The fibers can serve as synthetic blood vessels, ducts, or nerves.
    Type: Grant
    Filed: July 1, 2015
    Date of Patent: February 19, 2019
    Assignee: The United States of America, as represented by the Secretary of the Navy
    Inventors: André A. Adams, Michael Daniele, Frances S. Ligler
  • Patent number: 10206663
    Abstract: A sample holder that enables mid-infrared spectroscopy of a test sample using mid-infrared light is disclosed. The sample holder includes an inlet port and a sample chamber that comprises a sample region and a capillary channel that fluidically couples the inlet port and the sample region. The capillary channel is characterized by a higher capillary force than the sample region. As a result, when the inlet port is put in contact with a liquid containing the test sample, the liquid is drawn into the sample region without the formation of bubbles that could obscure the optical analysis. In some embodiments, the inlet port is the free end of a draw tube having an outer diameter that is smaller than the minimum spacing between pain receptors at a draw site on a patient, which mitigates pain felt by the patient due to insertion of the draw tube at the draw site.
    Type: Grant
    Filed: January 14, 2016
    Date of Patent: February 19, 2019
    Assignee: Roc8Sci Co.
    Inventor: Frank Thomas Hartley
  • Patent number: 10156487
    Abstract: A tactile sensor includes a first insulating layer having a first array of electrically conductive strips embedded therein and extending in a first direction. An intermediate layer of conductive soft polymer material is positioned above the first insulating layer and the first array of said electrically conductive strips. A second insulating layer having a second array of electrically conductive strips embedded therein, which extend in a second direction which is different than the first direction, is positioned above the intermediate layer. The first array of electrically conductive strips are connected to the second array of electrically conductive strips, and both the first and second array of electrically conductive strips are also connected to an impedance measuring device.
    Type: Grant
    Filed: September 20, 2016
    Date of Patent: December 18, 2018
    Assignee: The University of Akron
    Inventors: Jae-Won Choi, Erik Daniel Engeberg, Morteza Vatani, Ho-Chan Kim, Thomas Swiger
  • Patent number: 10132783
    Abstract: A method is presented for fabricating a fluidic system for a gas chromatograph. The method includes: microfabricating a portion of a fluidic system of a gas chromatograph on a substrate using a first mask; microfabricating a portion of the fluidic system of the gas chromatograph using a second mask; and microfabricating a portion of the fluidic system of the gas chromatograph using a third mask, such that the first mask, the second mask and the third mask are different from each other and the microfabricating of the fluidic system of the gas chromatograph is completed using only the first, second and third masks. A gas chromatograph wherein a microfabricated Knudsen pump is arranged to operate in a first direction to draw carrier gas into a preconcentrator and in a second direction to draw gas out of the preconcentrator.
    Type: Grant
    Filed: May 16, 2014
    Date of Patent: November 20, 2018
    Assignee: The Regents of The University of Michigan
    Inventors: Yogesh B. Gianchandani, Yutao Qin
  • Patent number: 10107781
    Abstract: A method for separating a plurality of deformable objects, such as biological cells or biological supramolecules such as DNA, in a liquid medium by use of an electrophoretic technique combined with hydrodynamic forces. The deformable objects are introduced into a channel, having a flow axis and a cross section at right angles to the flow axis, with the minimum size of the cross section being less than or equal to 50 pm; A hydrodynamic flow is defined a in the channel together with the application of an electric field in the channel, making it possible to move the deformable objects in the channel according to the flow axis and to separate them along the flow axis. A device suitable for implementing this method. The electrolyte used for the electrophoretic separation may be a non-Newtonian fluid with viscoelastic properties.
    Type: Grant
    Filed: July 29, 2013
    Date of Patent: October 23, 2018
    Assignee: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
    Inventors: Aurélien Bancaud, Hubert Ranchon
  • Patent number: 10073054
    Abstract: Isotachophoresis (ITP) is exploited to control various aspects of chemical reactions. In a first aspect, at least one of the reactants of a chemical reaction is confined to an ITP zone, but the resulting product of the chemical reaction is separated from this ITP zone by the ITP process. In a second aspect, one or more reactants of a chemical reaction are confined to an ITP zone, and one or more other reactants of the chemical reaction are not confined to this ITP zone. In a third aspect, ITP is employed to confine at least one reactant of a chemical reaction to an ITP zone, and at least one reactant of the chemical reaction is delivered to the ITP zone in two or more discrete doses. These aspects are especially relevant to performing polymerase chain reactions using chemical denaturants as opposed to thermal cycling.
    Type: Grant
    Filed: March 25, 2016
    Date of Patent: September 11, 2018
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Juan G. Santiago, Alexandre Persat
  • Patent number: 10052631
    Abstract: Microfluidic devices for the rapid and automated processing of sample populations are provided. Described are multiplexer microfluidic devices configured to serially deliver a plurality of distinct sample populations to a sample processing element rapidly and automatically, without cross-contaminating the distinct sample populations. Also provided are microfluidic sample processing elements that can be used to rapidly and automatically manipulate and/or interrogate members of a sample population. The microfluidic devices can be used to improve the throughput and quality of experiments involving model organisms, such as C. elegans.
    Type: Grant
    Filed: March 5, 2014
    Date of Patent: August 21, 2018
    Assignee: Board of Regents, The University of Texas System
    Inventors: Adela Ben-Yakar, Navid Ghorashian, Sertan Kutal Gökçe, Sam Xun Guo, William Neil Everett, Frederic Bourgeois
  • Patent number: 9998537
    Abstract: In one aspect, a method includes configuring a kernel driver to record data block changes of a logical unit in a bit map and using the bitmap to perform an incremental backup of the logical unit. In another aspect, an apparatus includes electronic hardware circuitry configured to configure a kernel driver to record data block changes of a logical unit in a bit map and use the bitmap to perform an incremental backup of the logical unit. In a further aspect, an article includes a non-transitory computer-readable medium that stores computer-executable instructions. The instructions cause a machine to configure a kernel driver to record data block changes of a logical unit in a bit map and use the bitmap to perform an incremental backup of the logical unit.
    Type: Grant
    Filed: March 31, 2015
    Date of Patent: June 12, 2018
    Assignee: EMC IP HOLDING COMPANY LLC
    Inventors: Mark A. O'Connell, Helen S. Raizen, Brad Bowlin