Capillary Electrophoresis Patents (Class 204/451)
  • Patent number: 10295545
    Abstract: Flow apparatuses comprising a separation channel, a downstream flow separator, a detection zone, an observation zone, and a waste channel. The separation channel has first and second flows in contact and allows lateral movement of components between contacting first and second flows. The downstream flow separator is in communication with the separation channel and diverts a part of the first fluid flow, the second fluid flow, or both, from the separation channel. The detection zone comprises a detection channel downstream of and in communication with the flow separator and configured to receive a plurality of diverted flows from the flow separator and a label channel configured to label the diverted flows from the flow separator. The observation zone is configured to record an analytical signal indicative of the quantity and the electrical properties of the component. The waste channel is at the downstream end of the observation zone.
    Type: Grant
    Filed: March 15, 2018
    Date of Patent: May 21, 2019
    Assignee: Cambridge Enterprise Limited
    Inventors: Emma Yates, Christopher Dobson, Therese Herling, Tuomas Knowles
  • Patent number: 10274460
    Abstract: The present disclosure relates, in some embodiments, to an apparatus for conducting a capillary electrophoresis assay. The apparatus can comprise a capillary array comprising an anode end and a cathode end, the capillary array provided in a housing further comprising a reservoir configured to house a separation medium and an anode buffer. The system can also comprise an injection mechanism configured to deliver sample to the cathode end of the capillary array, and a temperature control zone, wherein the temperature control zone is configured to control the temperature of the interior of the housing.
    Type: Grant
    Filed: March 6, 2015
    Date of Patent: April 30, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Ian Walton, Achim Karger, Alexander Khorlin, Michael Simon, Adam Sannicandro, Alexander Dukhovny, Robert Cobene, Dan Kline, John Dixon
  • Patent number: 10258982
    Abstract: A combined-blade open flow path device is a fluid flow path device where flow paths are adjacent to each other. The combined-blade open flow path device comprises a substrate configured to constitute a bottom portion of the flow paths; and blades erected on a surface of the substrate, the blades being configured to constitute side walls of the flow paths, wherein the blades are erected in groups, each of the groups extending from an upstream side to a downstream side in a flow direction of a fluid with a space between each of the blades in the flow direction of the fluid in each of the groups for making conduction of the fluid between the adjacent flow paths possible, and wherein one end of one of the flow paths is configured to be in contact with the fluid and is configured to make a flow of the fluid possible.
    Type: Grant
    Filed: April 22, 2015
    Date of Patent: April 16, 2019
    Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Takahiko Hariyama, Daisuke Ishii, Masatsugu Shimomura, Ko Okumura, Marie Tani
  • Patent number: 10247710
    Abstract: Certain embodiments described herein are directed to chromatography systems that include a microfluidic device and that implement one or more methods to direct sample to a desired fluid flow path. The methods can be used to backflush a sample to a desired fluid flow path to select certain analytes within a sample.
    Type: Grant
    Filed: October 21, 2013
    Date of Patent: April 2, 2019
    Assignee: PerkinElmer Health Sciences, Inc.
    Inventor: Andrew Tipler
  • Patent number: 10206663
    Abstract: A sample holder that enables mid-infrared spectroscopy of a test sample using mid-infrared light is disclosed. The sample holder includes an inlet port and a sample chamber that comprises a sample region and a capillary channel that fluidically couples the inlet port and the sample region. The capillary channel is characterized by a higher capillary force than the sample region. As a result, when the inlet port is put in contact with a liquid containing the test sample, the liquid is drawn into the sample region without the formation of bubbles that could obscure the optical analysis. In some embodiments, the inlet port is the free end of a draw tube having an outer diameter that is smaller than the minimum spacing between pain receptors at a draw site on a patient, which mitigates pain felt by the patient due to insertion of the draw tube at the draw site.
    Type: Grant
    Filed: January 14, 2016
    Date of Patent: February 19, 2019
    Assignee: Roc8Sci Co.
    Inventor: Frank Thomas Hartley
  • Patent number: 10208289
    Abstract: A fiber includes one or more layers of polymer surrounding a central lumen, and living animal cells disposed within the lumen and/or within at least one of the one or more layers, wherein the fiber has an outer diameter of between 5 and 8000 microns and wherein each individual layer of polymer has a thickness of between 0.1 and 250 microns. Also disclosed are model tissues including such fibers, and method of making such fibers. The fibers can serve as synthetic blood vessels, ducts, or nerves.
    Type: Grant
    Filed: July 1, 2015
    Date of Patent: February 19, 2019
    Assignee: The United States of America, as represented by the Secretary of the Navy
    Inventors: André A. Adams, Michael Daniele, Frances S. Ligler
  • Patent number: 10156487
    Abstract: A tactile sensor includes a first insulating layer having a first array of electrically conductive strips embedded therein and extending in a first direction. An intermediate layer of conductive soft polymer material is positioned above the first insulating layer and the first array of said electrically conductive strips. A second insulating layer having a second array of electrically conductive strips embedded therein, which extend in a second direction which is different than the first direction, is positioned above the intermediate layer. The first array of electrically conductive strips are connected to the second array of electrically conductive strips, and both the first and second array of electrically conductive strips are also connected to an impedance measuring device.
    Type: Grant
    Filed: September 20, 2016
    Date of Patent: December 18, 2018
    Assignee: The University of Akron
    Inventors: Jae-Won Choi, Erik Daniel Engeberg, Morteza Vatani, Ho-Chan Kim, Thomas Swiger
  • Patent number: 10132783
    Abstract: A method is presented for fabricating a fluidic system for a gas chromatograph. The method includes: microfabricating a portion of a fluidic system of a gas chromatograph on a substrate using a first mask; microfabricating a portion of the fluidic system of the gas chromatograph using a second mask; and microfabricating a portion of the fluidic system of the gas chromatograph using a third mask, such that the first mask, the second mask and the third mask are different from each other and the microfabricating of the fluidic system of the gas chromatograph is completed using only the first, second and third masks. A gas chromatograph wherein a microfabricated Knudsen pump is arranged to operate in a first direction to draw carrier gas into a preconcentrator and in a second direction to draw gas out of the preconcentrator.
    Type: Grant
    Filed: May 16, 2014
    Date of Patent: November 20, 2018
    Assignee: The Regents of The University of Michigan
    Inventors: Yogesh B. Gianchandani, Yutao Qin
  • Patent number: 10107781
    Abstract: A method for separating a plurality of deformable objects, such as biological cells or biological supramolecules such as DNA, in a liquid medium by use of an electrophoretic technique combined with hydrodynamic forces. The deformable objects are introduced into a channel, having a flow axis and a cross section at right angles to the flow axis, with the minimum size of the cross section being less than or equal to 50 pm; A hydrodynamic flow is defined a in the channel together with the application of an electric field in the channel, making it possible to move the deformable objects in the channel according to the flow axis and to separate them along the flow axis. A device suitable for implementing this method. The electrolyte used for the electrophoretic separation may be a non-Newtonian fluid with viscoelastic properties.
    Type: Grant
    Filed: July 29, 2013
    Date of Patent: October 23, 2018
    Assignee: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
    Inventors: Aurélien Bancaud, Hubert Ranchon
  • Patent number: 10073054
    Abstract: Isotachophoresis (ITP) is exploited to control various aspects of chemical reactions. In a first aspect, at least one of the reactants of a chemical reaction is confined to an ITP zone, but the resulting product of the chemical reaction is separated from this ITP zone by the ITP process. In a second aspect, one or more reactants of a chemical reaction are confined to an ITP zone, and one or more other reactants of the chemical reaction are not confined to this ITP zone. In a third aspect, ITP is employed to confine at least one reactant of a chemical reaction to an ITP zone, and at least one reactant of the chemical reaction is delivered to the ITP zone in two or more discrete doses. These aspects are especially relevant to performing polymerase chain reactions using chemical denaturants as opposed to thermal cycling.
    Type: Grant
    Filed: March 25, 2016
    Date of Patent: September 11, 2018
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Juan G. Santiago, Alexandre Persat
  • Patent number: 10052631
    Abstract: Microfluidic devices for the rapid and automated processing of sample populations are provided. Described are multiplexer microfluidic devices configured to serially deliver a plurality of distinct sample populations to a sample processing element rapidly and automatically, without cross-contaminating the distinct sample populations. Also provided are microfluidic sample processing elements that can be used to rapidly and automatically manipulate and/or interrogate members of a sample population. The microfluidic devices can be used to improve the throughput and quality of experiments involving model organisms, such as C. elegans.
    Type: Grant
    Filed: March 5, 2014
    Date of Patent: August 21, 2018
    Assignee: Board of Regents, The University of Texas System
    Inventors: Adela Ben-Yakar, Navid Ghorashian, Sertan Kutal Gökçe, Sam Xun Guo, William Neil Everett, Frederic Bourgeois
  • Patent number: 9995668
    Abstract: Microfluidic system comprising a space for containing a liquid and at least one lateral chamber in communication with said space, said lateral chamber containing a metal electrode. The lateral chamber and the space are designed to be filled by the same or different liquid when the system is active.
    Type: Grant
    Filed: February 1, 2007
    Date of Patent: June 12, 2018
    Assignee: Ecole Polytechnique Fédérale de Lausanne (EPFL)
    Inventors: Philippe Renaud, Pontus Linderholm, Thomas Braschler, Nicolas Demierre, Urban Seger
  • Patent number: 9998537
    Abstract: In one aspect, a method includes configuring a kernel driver to record data block changes of a logical unit in a bit map and using the bitmap to perform an incremental backup of the logical unit. In another aspect, an apparatus includes electronic hardware circuitry configured to configure a kernel driver to record data block changes of a logical unit in a bit map and use the bitmap to perform an incremental backup of the logical unit. In a further aspect, an article includes a non-transitory computer-readable medium that stores computer-executable instructions. The instructions cause a machine to configure a kernel driver to record data block changes of a logical unit in a bit map and use the bitmap to perform an incremental backup of the logical unit.
    Type: Grant
    Filed: March 31, 2015
    Date of Patent: June 12, 2018
    Assignee: EMC IP HOLDING COMPANY LLC
    Inventors: Mark A. O'Connell, Helen S. Raizen, Brad Bowlin
  • Patent number: 9969964
    Abstract: The invention generally relates to a cell culture system for coculturing non-neoplastic and neoplastic cells on a planar member which more faithfully mimics the in vivo geometry of a lumen or a cavity.
    Type: Grant
    Filed: December 19, 2014
    Date of Patent: May 15, 2018
    Assignee: PURDUE RESEARCH FOUNDATION
    Inventors: Sophie Andree Lelievre, Pierre-Alexandre Vidi, James Francis Leary, Teimour Maleki-Jafarabadi
  • Patent number: 9953817
    Abstract: An ion transfer tube assembly, a mass spectrometry system, and a method for providing an ion stream to an ion detection device are described that include using an ion transfer tube that provides a coaxial sheath gas flow. In an implementation, an ion transfer tube assembly includes an ion transfer tube for delivering the ion stream, where a sheath gas flows through the ion transfer tube, and where the ion transfer tube receives the ion stream from a first conduit coupled to an ion source; a pump fluidly coupled to the ion transfer tube, where the pump causes the sheath gas to flow through the ion transfer tube, where the ion stream is separated from the ion transfer tube walls by the coaxial sheath gas flow, and where the ion stream is received by a second conduit coupled to the ion detection device.
    Type: Grant
    Filed: April 22, 2016
    Date of Patent: April 24, 2018
    Assignee: Smiths Detection Inc.
    Inventors: Vadym Berkout, Douglas J. Green
  • Patent number: 9927397
    Abstract: Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.
    Type: Grant
    Filed: November 26, 2014
    Date of Patent: March 27, 2018
    Assignee: STC.UNM
    Inventors: Steven R. J. Brueck, Jeremy Scott Edwards, Alexander Neumann, Yuliya Kuznetsova, Edgar A. Mendoza, C. Jeffrey Brinker
  • Patent number: 9901926
    Abstract: The present invention is notably directed to a microfluidic chip (1, 1a) comprising: a flow path (22) defined by a hydrophilic surface; a liquid input (24, 24a, 24b) on one side of the flow path; at least one electrical circuit (62), hereafter DEP circuit, comprising at least one pair of dielectrophoretic electrodes (E21, E22), hereafter DEP electrodes, wherein: each of the DEP electrodes extends transverse to the flow path; and the DEP circuit is configured to generate a dielectrophoretic force, hereafter DEP force, at the level of the DEP electrodes. The chip may further include one or more electroosmotic circuits. The present invention is further directed to methods of operation of such a microfluidic chip.
    Type: Grant
    Filed: June 18, 2014
    Date of Patent: February 27, 2018
    Assignee: International Business Machines Corporation
    Inventors: Jaione Tirapu Azpiroz, Emmanuel Delamarche, Tobias Guenzler, Govind Kaigala, Yuksel Temiz, Tino Treiber
  • Patent number: 9903835
    Abstract: A fully automated high-precision capillary electrophoresis instrument, comprising an electrophoresis system, a sample injection flow path, and an automatic sampling flow path; the sampling flow path comprising a shunt waste bottle, which is connected to a four-way connector, a four-way sample injection valve and a buffer syringe pump; the automatic sampling flow path comprises a sampling needle, a sample tray, a cleaning tank, reagent bottles, a buffer tube, a six-channel liquid dispenser, and a syringe pump. The described capillary electrophoresis instrument has a fast sample injection speed, high accuracy, good reproducibility, and can be widely used in automated analysis of different substances by capillary electrophoresis.
    Type: Grant
    Filed: May 8, 2014
    Date of Patent: February 27, 2018
    Inventor: Chao Yan
  • Patent number: 9895690
    Abstract: Provided is a microfluidic chip, which comprises a substrate and a cover sheet, wherein a microreactor array is arranged on the substrate and comprises at least one main channel (401) and at least two micro cells (402) connected to the main channel (401). The microfluidic chip also comprises at least one local temperature control device, which is used for heating the main channel (401) or cooling the micro cells (402). The use of the microfluidic chip ensures uniformity and independency of the micro cells (402). Also provided is an application of the microfluidic chip in biological detection or medical inspection.
    Type: Grant
    Filed: August 23, 2013
    Date of Patent: February 20, 2018
    Assignees: Capitalbio Corporation, Tsinghua University
    Inventors: Guohao Zhang, Guoliang Huang, Can Wang, Su Guo, Lei Wang, Wanli Xing, Jing Cheng
  • Patent number: 9897572
    Abstract: Apparatuses and associated methods for manipulating an assembly of glass slides employed in cellular assay processes are provided. Each apparatus can accommodate at least one removable rack of slides to undergo electrophoresis in a comet assay. The slides can remain in the same apparatus while being subjected to a sequence of fluid staining and washing with temperature control, advantageously shortening the amount of time required for processing the slides by keeping them in the same work station for the entire duration of the assay.
    Type: Grant
    Filed: December 2, 2016
    Date of Patent: February 20, 2018
    Assignee: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES
    Inventors: Marcus S. Cooke, Shekhar Bhansali, Mahsa Karbaschi, Pratikkumar Shah
  • Patent number: 9869669
    Abstract: Provided is a sensor platform that includes a substrate, a plurality of nanochannels disposed on the substrate, and a plurality of electrodes, a waveguide disposed on the substrate and an analysis chamber and a reference chamber disposed on the substrate. Each electrode extends substantially across a width of the plurality of nanochannels. At least one analysis optical resonator is disposed in the analysis chamber and is optically coupled to at least a portion of the waveguide. The at least one analysis optical resonator is in fluid communication with at least one of the plurality of nanochannels. At least one reference optical resonator is disposed in the reference chamber and is optically coupled to at least a portion of the waveguide. The at least one reference optical resonator is in fluid communication with at least one other of the plurality of nanochannels.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: January 16, 2018
    Assignees: STC.UNM, WASHINGTON STATE UNIVERSITY
    Inventors: Sang M. Han, Cornelius F. Ivory, Mani Hossein-Zadeh
  • Patent number: 9855684
    Abstract: Embodiments of electrophoresis systems, devices, and associated methods of analysis are described herein. In one embodiment, an electrophoresis device includes a first electrode having a first polarity, a second electrode having a second polarity, and a substrate. The substrate includes a first channel having a first section with a first cross-sectional area and a second section with a second cross-sectional area. The first end is electrically coupled to the first electrode, and the second end is electrically coupled to the second electrode. The first cross-sectional area is greater than the second cross-sectional area in a first dimension and in a second dimension generally orthogonal to the first dimension.
    Type: Grant
    Filed: February 10, 2012
    Date of Patent: January 2, 2018
    Assignee: Washington State University
    Inventor: Cornelius F. Ivory
  • Patent number: 9857333
    Abstract: Individual biological micro-objects can be deterministically selected and moved into holding pens in a micro-fluidic device. A flow of a first liquid medium can be provided to the pens. Physical pens can be structured to impede a direct flow of the first medium into a second medium in the pens while allowing diffusive mixing of the first medium and the second medium. Virtual pens can allow a common flow of medium to multiple ones of the pens.
    Type: Grant
    Filed: October 22, 2013
    Date of Patent: January 2, 2018
    Assignee: Berkeley Lights, Inc.
    Inventors: Kevin T. Chapman, Igor Y. Khandros, Gaetan L. Mathieu, J. Tanner Nevill, Ming C. Wu
  • Patent number: 9833781
    Abstract: An example micro-fluidic device includes a micro-fluidic channel having an inner surface and a plurality of pillars positioned along the inner surface. The device further includes a plurality of power supplies connected to the pillars. Another example micro-fluidic device includes a micro-fluidic channel having an inner surface and a plurality of pillars positioned along the inner surface. The device further includes a power supply. The pillars are grouped into at least two groups of pillars, each group of pillars including at least two pillars, and all pillars of at least one group of pillars are connected to the power supply. In another example, a sensing system for detecting bioparticles includes a micro-fluidic device, wherein a surface of each pillar comprises functionalized plasmonic nanoparticles or functionalized SERS nanoparticles, a radiation source for radiating the micro-fluidic device, and a detector for detecting SERS signals or surface plasmon resonance.
    Type: Grant
    Filed: May 17, 2014
    Date of Patent: December 5, 2017
    Assignee: IMEC
    Inventors: Chengjun Huang, Chengxun Liu, Liesbet Lagae, Paolo Fiorini, Benjamin Jones
  • Patent number: 9815059
    Abstract: The invention presents a microfluidic device that provides investigation of distance dependent interactions between cells and various factors. A method that uses the device to determine distance dependent interactions between cells and various factors and agents that can change these interactions is also presented.
    Type: Grant
    Filed: September 29, 2014
    Date of Patent: November 14, 2017
    Inventor: Devrim Pesen Okvur
  • Patent number: 9791453
    Abstract: A mechanism is provided for utilizing a nanodevice to distinguish molecules with different structure. The molecules translocate through or across a nanochannel filled with a electrolyte solution. An electrical signal through the nanochannel is measured for every translocation event. Inner surfaces of the nanochannel include a functional layer, which is a coating to functionalize the nanochannel, in which the functional layer is configured to interact with predetermined ones of the molecules during translocation events. It is determined that a combination of at least two different molecules is formed based on predetermined ones of the molecules interacting with the functional layer to change the electrical signal and/or change a translocation time for the translocation event.
    Type: Grant
    Filed: December 26, 2012
    Date of Patent: October 17, 2017
    Assignees: INTERNATIONAL BUSINESS MACHINES CORPORATION, THE UNIVERSITY OF MELBOURNE, FLOREY INSTITUTE OF NEUROSCIENCE AND MENTAL HEALTH
    Inventors: Matthew Downton, Natalie Gunn, Stefan Harrer, Priscilla Rogers, John Wagner, Ross Bathgate, Daniel Scott, Stan Skafidas
  • Patent number: 9783826
    Abstract: The invention generally provides methods for producing recombinant AAV viral particles using cells grown in suspension. The invention provides recombinant AAV particles for use in methods for delivering genes encoding therapeutic proteins, and methods for using the recombinant AAV particles in gene therapy.
    Type: Grant
    Filed: November 21, 2012
    Date of Patent: October 10, 2017
    Assignee: Applied Genetic Technologies Corporation
    Inventors: David R. Knop, Darby Thomas, Gabor Veres
  • Patent number: 9766216
    Abstract: This invention provides methods and compositions, e.g., to reduce interference from non-specific binding sample constituents in a migration shift assay. Interference due to non-specific binding of sample constituents to an affinity substance (e.g., an affinity molecule or a conjugate of an affinity molecule and a charged carrier molecule) is prevented by, e.g., binding the constituents to charged polymers such as heparin sulfate. The present invention also provides methods to concentrate an analyte of interest with high concentration and to detect the analyte with high sensitivity, and further to optimize the reaction conditions for easily concentrating the analyte. Such objects of the present invention are attained, for example, by concentrating a complex of the analyte and a conjugate which is formed by contacting the analyte in a sample with an affinity molecule bound to a charged carrier molecule such as DNA.
    Type: Grant
    Filed: April 8, 2004
    Date of Patent: September 19, 2017
    Assignee: Wako Pure Chemical Industries, Ltd.
    Inventors: H. Garrett Wada, Irina G. Kazakova, Yutaka Miki, Toshinari Ohashi, Futoshi Kanke
  • Patent number: 9745949
    Abstract: The invention is directed to a microfluidic probe head (100) with a base layer (120) comprising: at least two processing liquid microchannel (123, 124) in fluid communication with a processing liquid aperture (121, 122) on a face of the base layer; and an immersion liquid microchannel (223, 224) in fluid communication with a immersion liquid aperture (221, 222) on a face of the base layer, wherein the microfluidic probe head is configured to allow, in operation, processing liquid provided through the processing liquid aperture to merge into immersion liquid provided through the immersion liquid aperture. An additional layer can be provided to close the microchannels. Such a multilayered head is compact and easier to fabricate than heads made with unitary construction. The head can further be interfaced with tubing using e.g. a standard fitting for tubing port.
    Type: Grant
    Filed: October 18, 2011
    Date of Patent: August 29, 2017
    Assignee: International Business Machines Corporation
    Inventors: Emmanuel Delamarche, Govind Kaigala, Robert Lovchik
  • Patent number: 9733232
    Abstract: A technique relates to stretching an extensible molecule. The molecule moves through an array of pillars in a flow direction, where the array has an interface connecting a first pillar region and a second pillar region. Stretching the molecule by traversing the molecule in the flow direction through the interface connecting the first pillar region to the second pillar region, such that a first end and a second end of the molecule straddle a straddle pillar, thereby causing the first end to extend along a first path in the second and the second end to extend along a second path. Traversing the molecule stretches the first end and the second end along two different paths. The molecule is further traversed through the array such that the second end follows the first end along the first path, where the stretching causes the molecule to be in an uncoiled state.
    Type: Grant
    Filed: January 25, 2016
    Date of Patent: August 15, 2017
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Gustavo A. Stolovitzky, Chao Wang
  • Patent number: 9723817
    Abstract: Disclosed herein are devices, systems, and methods for measuring an electropharyngeogram (EPG) in living organisms. In some embodiments, the devices and systems disclosed herein include an array for sorting and immobilizing an organism (such as a nematode) for measurement of an EPG and/or optical imaging. Also disclosed are methods for identifying therapeutic or toxic compounds utilizing the disclosed devices and systems. In some embodiments, the methods include screening for compounds with anthelmintic activity, toxicity (for example HERG channel blockers), or candidate drugs for treatment of a variety of human and/or animal diseases.
    Type: Grant
    Filed: November 9, 2012
    Date of Patent: August 8, 2017
    Assignee: State of Oregon Acting by and through the State Board of Higher Education on behalf of the University of Oregon
    Inventor: Shawn Lockery
  • Patent number: 9689035
    Abstract: The present invention relates generally to 3?-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.
    Type: Grant
    Filed: June 21, 2016
    Date of Patent: June 27, 2017
    Assignee: LASERGEN, INC.
    Inventors: Brian P. Stupi, Hong Li, Weidong Wu, Megan N. Hersh, David Hertzog, Sidney E. Morris, Michael L. Metzker
  • Patent number: 9683960
    Abstract: The present invention provides a system including: a peptide nucleic acid (PNA) molecule; a DNA molecule, an RNA molecule, or a combination thereof; and an Isotachophoresis (ITP) system. Furthermore, the invention provides a method for sequence-specifically separating and/or identifying a nucleic acid molecule of interest by utilizing the system of the invention to separate and possibly label and/or detect a nucleic acid molecule of interest.
    Type: Grant
    Filed: August 18, 2014
    Date of Patent: June 20, 2017
    Assignee: Technion Research and Development Foundation Limited
    Inventors: Moran Bercovici, Nadya Ostromohov, Ortal Schwartz
  • Patent number: 9670541
    Abstract: An array of transportable particle sets is used in a microfluidic device for performing chemical reactions in the microfluidic device. The microfluidic device comprises a main channel and intersecting side channels, the main channel and side channels forming a plurality of intersections. The array of particle sets is disposed in the main channel, and the side channels are coupled to reagents. As the particle sets are transported through the intersections of the main channel and the side channels, reagents are flowed through the side channels into contact with each array member (or selected array members), thereby providing a plurality of chemical reactions in the microfluidic system.
    Type: Grant
    Filed: August 11, 2015
    Date of Patent: June 6, 2017
    Assignee: CALIPER LIFE SCIENCES, INC.
    Inventors: Tammy Burd Mehta, Anne R. Kopf-Sill, J. Wallace Parce, Andrea W. Chow, Luc J. Bousse, Michael R. Knapp, Theo T. Nikiforov, Steve Gallagher
  • Patent number: 9581528
    Abstract: Methods and apparatus are described for the processing (for example washing, incubation, etc.
    Type: Grant
    Filed: February 20, 2014
    Date of Patent: February 28, 2017
    Assignee: Menarini Silicon Biosystems S.p.A.
    Inventor: Nicolò Manaresi
  • Patent number: 9574232
    Abstract: Devices and methods for controlling reversible chemical reactions at solid-liquid interfaces are disclosed. In particular, the invention relates to a method of increasing reaction rates by concentrating a target molecule in a liquid phase in the region of a reactant or ligand immobilized on a solid followed by removal of the liquid phase and replacement with an immiscible phase, such as an immiscible gas or liquid to impede the reverse reaction. Devices for performing this method to increase the rates and degree of completion of kinetically limited ligand binding or nucleic acid hybridization reactions in affinity chromatography and microarray applications are also disclosed.
    Type: Grant
    Filed: February 24, 2015
    Date of Patent: February 21, 2017
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Crystal Han, Juan G. Santiago, Viktor Shkolnikov
  • Patent number: 9546996
    Abstract: The present invention provides an apparatus for analyzing the sequence of nucleotides in a nucleic acid sample, said apparatus comprising a substrate and a plurality of nanopores provided therein suitable for the passage of nucleic acid molecules therethrough; at least one sample holding chamber disposed upstream of the inlet of said nanopores, at least one detection window juxtaposed within or downstream of the outlet of each nanopore adapted to detect a property characteristic of one or more detectable elements associated with the nucleic acid as each nucleic acid molecule passes therethrough and a detector adapted to generate a data stream characteristic of the various detection events occurring in the detection window characterized in that the apparatus further comprises a means located within the sample holding chamber adapted to increase the local concentration of the nucleic acid sample adjacent the inlet of the nanopores relative to the bulk concentration thereof.
    Type: Grant
    Filed: July 9, 2013
    Date of Patent: January 17, 2017
    Assignee: BASE4 INNOVATION LTD.
    Inventors: Bruno Flavio Nogueira de Sousa Soares, Cameron Alexander Frayling, Barnaby Balmforth, Michele Amasio
  • Patent number: 9546932
    Abstract: A pneumatically driven portable assay cartridge having analyte capture regions associated with microfluidic channels within its interior, the portable cartridge having pneumatic ports clampable against pneumatic ports of an operating instrument for controlled application of positive pressure and vacuum to pneumatic operating channels within the cartridge, the cartridge having a well for receiving sample from a user and microfluidic channels that include pneumatically operated pistons and valves controllable by the pneumatic operating channels to cause all flows of the assay from the reservoirs through reaction regions within the cartridge to on-board waste reservoir during conduct of the assay.
    Type: Grant
    Filed: September 6, 2014
    Date of Patent: January 17, 2017
    Assignee: CyVek, Inc.
    Inventors: Martin A. Putnam, Jeffrey T. Branciforte, Charles O. Stanwood
  • Patent number: 9506894
    Abstract: The present invention provides a method and apparatus for controlling the moving speed of a substance, both of which can adjust the moving speed of a substance to a desired speed. The control method and control apparatus cause a substance to pass through an internal space, in which an electro-osmotic flow is generated, of a surround electrode formed so as to surround part of the moving path of the substance, whereby the control method and control apparatus change the moving speed of the substance.
    Type: Grant
    Filed: August 26, 2013
    Date of Patent: November 29, 2016
    Assignee: QUANTUM BIOSYSTEMS INC.
    Inventors: Tomoji Kawai, Soh Ryuzaki, Masateru Taniguchi
  • Patent number: 9465014
    Abstract: The invention provides a sheath-flow interface for producing electrospray from a capillary. The electrospray generated by the interface can be used as the source of ions for mass spectrometry. Electrokinetic flow in the interface can move a sheath liquid past the end of a capillary so as to mix with an analyte effluent discharged from the capillary. The sheath liquid and analyte mixture can be directed to an electrospray emitter to generate an electrospray.
    Type: Grant
    Filed: April 5, 2015
    Date of Patent: October 11, 2016
    Assignee: University of Notre Dame du Lac
    Inventors: Norman Dovichi, Liangliang Sun, Guijie Zhu
  • Patent number: 9451354
    Abstract: A device having one or more an acoustic modules. The acoustic module includes an acoustic element and a cavity that is acoustically coupled to the acoustic element. The module also includes a first conductive element that is configured to generate a first surface charge on a first region of an interior surface of the cavity. A second conductive element is configured to generate a second surface charge on a second region of the interior surface of the cavity. The first and second charge on the first and second regions of the interior surfaces of the cavity may be selectively applied to facilitate movement of a liquid held within the cavity.
    Type: Grant
    Filed: May 12, 2014
    Date of Patent: September 20, 2016
    Assignee: APPLE INC.
    Inventors: Stephen P. Zadesky, Fletcher R. Rothkopf, Ashley E. Fletcher
  • Patent number: 9442091
    Abstract: Described are methods of reducing the incidence and/or magnitude of artifacts in denaturing nucleic acid capillary electrophoresis (CE). Methods and systems described serve to dismiss non-denatured DNA from the tip of the capillary after sample injection and prior to electrophoretic separation of loaded nucleic acids. Among the methods disclosed are the application of a brief reverse-polarity pulse to the capillary prior to separation but after removal of the capillary from the sample reservoir, and transiently heating the capillary to cause expansion of the separation matrix after removal of the capillary from the sample reservoir.
    Type: Grant
    Filed: January 30, 2012
    Date of Patent: September 13, 2016
    Assignee: QIAGEN MANSFIELD, INC.
    Inventors: Sergey V. Voronov, Jork Nolling
  • Patent number: 9434937
    Abstract: Methods and systems for purifying cells and/or viruses are provided. The sample is added to a well disposed in a medium. A potential is applied across the medium to cause the contaminants to enter one or more walls of the well, and retain the cells and/or viruses in the well. The cells and/or viruses can be removed from the well, and optionally adhered or fixed to a surface, or detected. In one embodiment, the cells and/or viruses may be retained in the well by embedding in the medium. The medium including the embedded cells and/or viruses may be excised or otherwise removed and transferred to a glass slide or other solid surface.
    Type: Grant
    Filed: March 7, 2012
    Date of Patent: September 6, 2016
    Assignee: ACCELERATE DIAGNOSTICS, INC.
    Inventors: Steven W. Metzger, Kenneth Robert Hance
  • Patent number: 9409252
    Abstract: A microfluidic device for separating a sample by chromatography includes diffusion bonded metallic sheets joined together to create a hermetically sealed interface between each adjacent metallic sheet without the introduction of a secondary material. Enclosed within the diffusion bonded sheets is a separation channel accessible by at least one of an inlet or an outlet. The separation channel is packed with micrometer-sized particles serving as a stationary phase in a chromatographic separation. Wetted surfaces of the separation channel include a coating of an organic material at least one monolayer thick.
    Type: Grant
    Filed: March 25, 2011
    Date of Patent: August 9, 2016
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Bernard Bunner, Theodore A. Dourdeville
  • Patent number: 9387489
    Abstract: The present invention includes methods, devices and systems for isolating nanoparticulates, including nucleic acids, from biological samples. In various aspects, the methods, devices and systems may allow for a rapid procedure that requires a minimal amount of material and/or results in high purity isolation of biological components from complex fluids such as blood or environmental samples.
    Type: Grant
    Filed: April 7, 2015
    Date of Patent: July 12, 2016
    Assignee: BIOLOGICAL DYNAMICS, INC.
    Inventors: David Charlot, Juan Pablo Hinestrosa Salazar, Irina V. Dobrovolskaya, Kai Yang, Paul Swanson, Rajaram Krishnan
  • Patent number: 9362102
    Abstract: The invention provides a sheath-flow interface for producing electrospray from a capillary. The electrospray generated by the interface can be used as the source of ions for mass spectrometry. Electrokinetic flow in the interface can move a sheath liquid past the end of a capillary so as to mix with an analyte effluent discharged from the capillary. The sheath liquid and analyte mixture can be directed to an electrospray emitter to generate an electrospray.
    Type: Grant
    Filed: July 7, 2015
    Date of Patent: June 7, 2016
    Assignee: University of Notre Dame du Lac
    Inventors: Norman Dovichi, Liangliang Sun, Guijie Zhu
  • Patent number: 9354200
    Abstract: The present invention provides devices and methods for identification and/or quantitation of particles through detection of fluorescence labeled particles in an apparatus for differential charged particle mobility analysis and fluorescence detection.
    Type: Grant
    Filed: August 6, 2009
    Date of Patent: May 31, 2016
    Assignee: QUEST DIAGNOSTICS INVESTMENTS INCORPORATED
    Inventor: Michael P. Caulfield
  • Patent number: 9346045
    Abstract: An electrically conductive pipette tip having an elongated tubular body that has a bottom opening at the bottom end for the passage of liquid, and a top opening at the top end for the passage of air, and a passageway between the bottom opening and the top opening, wherein the body has at least one electrically-conductive region extending from the bottom opening to the top opening, and at least one transparent region consisting of plastic that extends from the passageway to the outside of the body.
    Type: Grant
    Filed: September 25, 2010
    Date of Patent: May 24, 2016
    Assignee: EPPENDORF AG
    Inventors: Michael Blumentritt, Nico Gülzow, Thomas Reimann, Christian Ziegmann, Matthias Haak
  • Patent number: 9322071
    Abstract: Disclosed is an economic method for concentrating virus and detecting virus, such that virus in a sample solution having low virus concentration can be concentrated with high efficiency within a short time. Particularly, the method comprising the steps of: (A) adding Concanavalin A (Con A) to a sample solution containing a virus, and reacting the added Concanavalin A with the virus in the sample solution to form a virus-Concanavalin A conjugate; and (B) separating the virus-Concanavalin A conjugate from the sample solution.
    Type: Grant
    Filed: September 29, 2014
    Date of Patent: April 26, 2016
    Assignees: Korea Basic Science Institute, Industry Foundation of Chonnam National University
    Inventors: Joseph Kwon, Jong-Soon Choi, Duwoon Kim, Hee-Min Lee
  • Patent number: 9228977
    Abstract: A portable electrophoretic contactless conductivity detection (C4D) system for analysis on a microfluidic chip houses in one embodiment a fluidic compartment for receiving the microfluidic chip, and four detection electrodes: first and second emitting electrodes, and first and second receiving electrodes. The first emitting electrode and the first receiving electrode are adjacent to a first channel wall of the microfluidic chip, and the second emitting electrode and the second receiving electrode are adjacent to a second channel wall, where the second channel wall is opposite to the first channel wall. In an embodiment, the electrodes are provided as portions of a removable cartridge cell.
    Type: Grant
    Filed: December 5, 2011
    Date of Patent: January 5, 2016
    Assignee: Agency for Science, Technology and Research
    Inventors: Kambiz Ansari Mahabadi, Isabel Rodriguez Fernandez, Chee Yen Lim, Jasmine Shu Ying Yuen