With Injection Patents (Class 204/453)
  • Patent number: 11479747
    Abstract: Systems and methods for the efficient agitation of tissue samples. A device may include a plurality of chambers that each receives samples therein. The plurality of chambers may be uniformly spaced with respect to a least one dimension, to form a one dimensional or two dimensional array. Each of the chambers may include an opening and an agitator device in fluid contact with the sample disposed within the chamber. The agitator devices may include a micromotor which provides rotational motion to a shaft and an impeller fixed to the shaft such that the impeller and the shaft rotate together upon provision of the rotational motion by the micromotor. The system may include an electrical energy source electrically coupled to the plurality of micromotors to rotate the impellers sufficient to agitate the sample as required for a particular activity (e.g., homogenization, lysis).
    Type: Grant
    Filed: February 1, 2018
    Date of Patent: October 25, 2022
    Inventors: Robert W. Doebler, Mark Brown, Tanya Ferguson, Bruce Irvine, James D. Sterling
  • Patent number: 11460436
    Abstract: The present disclosure provides component analysis methods including a measurement process and an analysis process.
    Type: Grant
    Filed: August 20, 2019
    Date of Patent: October 4, 2022
    Assignee: ARKRAY, INC.
    Inventor: Jin Yoshida
  • Patent number: 11446663
    Abstract: An apparatus and method for isolating bacterial particles in a sample using a container with material in temporary fluid blocking position to lower orifice in the container, a separation medium having an electrical conductivity lower than and physical density greater than that of the sample above the material that supports a sample concentrate after passing through the separation medium when exposed to centrifugal force, a heating element for liquefying the material to permit flow into a chamber past an electrode array that attracts and holds subject particles. The system allows rapid detection and isolation of particles from samples from animal, human, environmental sites, a bio-industrial reactor or a food or beverage production facility requiring relatively small volumes, short incubation times resulting in structurally intact particles for further analysis. Testing may be completed in a single unit that requires decreased technician manipulation, fewer steps and a decrease in cross-contamination.
    Type: Grant
    Filed: January 22, 2019
    Date of Patent: September 20, 2022
    Assignee: Aincobio LLC
    Inventors: Lorenzo D'Amico, Peter Russell Charles Gascoyne
  • Patent number: 11426735
    Abstract: A nucleic acid analysis apparatus includes a casing, a main frame, a fluid delivery unit, a thermal unit, a driving unit, and at least one optical unit. The casing has an upper casing and a lower casing. The main frame is disposed in the lower casing and has a chamber for mounting a cartridge therein. The fluid delivery unit is adapted to transport reagents within the cartridge for sample purification and/or nucleic acid extraction. The thermal unit is adapted to provide a predefined temperature for nucleic acid amplification. The driving unit is disposed in the lower casing and connected with the main frame, and includes a motion control unit capable of pressing the cartridge during sample purification and/or nucleic acid extraction and rotating the cartridge with a predefined program during nucleic acid amplification and/or detection. The optical unit includes plural optical components for detection.
    Type: Grant
    Filed: January 30, 2019
    Date of Patent: August 30, 2022
    Inventors: Qian Liang, Hao Yu, Haoyu Wei
  • Patent number: 11383236
    Abstract: Techniques are provided for a microfluidic chip for performing a microfluidic reaction. The microfluidic chip includes a rigid layer, a first polymer layer that forms a first surface of a microfluidic chamber within the microfluidic chip, a first adhesive layer disposed between the rigid layer and the first polymer layer, and a second polymer layer that forms a second surface of the microfluidic chamber, and a first port connecting an exterior of the microfluidic chip to the microfluidic chamber through at least the rigid layer, the first polymer layer, and the first adhesive layer. A first self-sealing valve is formed in the first polymer layer and disposed within the first port, the first self-sealing valve configured to seal directly against the rigid layer through a first gap in the first adhesive layer in response to pressure from thermal expansion of a fluid within the microfluidic chamber.
    Type: Grant
    Filed: November 12, 2018
    Date of Patent: July 12, 2022
    Inventor: Christopher Walker
  • Patent number: 11222779
    Abstract: Presented herein are apparatuses for use in capillary separations. An apparatus includes a coupling that integrates a capillary with a voltage source, a sheath liquid system, a fluid exit port, and a manifold. The coupling may be an elbow connector or equivalent. The manifold receives incident light from an incident light input, and emitted light is collected by a collected light output. The capillary enters the manifold at an input for the capillary, traverses the coupling, and terminates at the fluid exit port, for example an electrospray emitter. The capillary may also enter the manifold at an input for the capillary and terminates inside the manifold.
    Type: Grant
    Filed: May 16, 2019
    Date of Patent: January 11, 2022
    Assignee: GMJ Technologies, Inc.
    Inventor: Oluwatosin O. Dada
  • Patent number: 11198118
    Abstract: The present invention relates to an immunoaffinity device for capturing, isolating and purifying one or more analytes of interest present at high or low concentration in simple or complex matrices. The device is designed as an integrated modular unit that includes one or more analyte concentrator-microreactor devices anchored into a T-shaped support box, which is built-in or connected to a interchangeable cartridge-cassette of a capillary electrophoresis or liquid chromatography apparatus for the isolation, enrichment, derivatization, separation and characterization of small molecules and polymeric macromolecules, primarily protein and peptide biomarkers. The integrated modular unit is also designed to perform metabolic or bioactivity studies.
    Type: Grant
    Filed: March 28, 2018
    Date of Patent: December 14, 2021
    Inventors: Norberto Guzman, Daniel E. Guzman
  • Patent number: 11156681
    Abstract: A rotational force is applied to a sample tube by blowing of a drive gas to the sample tube. A spinning frequency of the sample tube is sequentially detected as a detection value. Acceleration control to gradually increase a pressure of the drive gas is executed until the detection value reaches a target value. In a process of the acceleration control, abnormality is determined based on a change with respect to time (difference: ?F) of the detection value. The determination is executed in a section of interest including a resonance frequency.
    Type: Grant
    Filed: December 19, 2019
    Date of Patent: October 26, 2021
    Assignee: JEOL Ltd.
    Inventors: Yuki Endo, Masahide Nishiyama
  • Patent number: 11110455
    Abstract: A microfluidic device for electrically activating a passive capillary stop valve, an apparatus and method are provided. The microfluidic device includes a first channel for containing a first fluid, and an output channel, wherein the first channel comprises a first interface with the output channel, and the first interface comprises a capillary stop valve characterised in that the microfluidic device also comprises a second channel for containing a second fluid, wherein the second channel comprises a second interface with the output channel, and the first channel and the second channel are electrically isolated from each other, and the first interface and the second interface are arranged relative to each other thereby being configured to activate fluid flow from the first channel into the output channel when a first fluid and a second fluid are present, and an electrical potential difference is applied between the first fluid and the second fluid.
    Type: Grant
    Filed: May 13, 2019
    Date of Patent: September 7, 2021
    Assignees: IMEC VZW, Katholieke Universiteit Leuven, KU LEUVEN R&D
    Inventors: Ujjal Barman, Benjamin Jones, Paolo Fiorini, David Mikaelian
  • Patent number: 11077441
    Abstract: Instruments and cartridges for processing droplets in emulsions containing biological entities such as cells. A method of such processing comprises providing a plurality of the entities in a fluid; preparing a droplet from the fluid; determining whether the droplet contains one or more entities of said plurality of entities, or whether said droplet does not contain a said entity; sorting said droplet dependent on an outcome of the determination; and dispensing the sorted droplet into a reservoir. The dispensing may comprise identifying and extracting the sorted droplet from a first fluidic flow path of said fluid by transferring the sorted droplet from into a second fluidic flow path and then ejecting the sorted droplet into a reservoir by applying pressure to the second fluidic flow path. The droplet contents may be tracked so that the contents of an individual droplet can be sorted, selectively dispensed, and retrieved.
    Type: Grant
    Filed: June 3, 2016
    Date of Patent: August 3, 2021
    Assignee: Sphere Fluidics Limited
    Inventors: Frank F. Craig, Marian Rehak, David Holmes, Clive A. Smith, Xin Liu, Giuseppe Benazzi, Xin Li, Vinayaka Pawate, Robert Frank Marchington, Richard Francis Day, Michael Stuart Hazell
  • Patent number: 11052398
    Abstract: Plastic electrophoresis separation chips are provided comprising a plurality of microfluidic channels and a detection window, where the detection window comprises a thin plastic; and the detection window comprises a detection region of each microfluidic channel. Such chips can be bonded to a support provided an aperture is provided in the support to allow detection of samples in the electrophoresis chip at the thin plastic detection window. Further, methods for electrophoretically separating and detecting a plurality of samples on the plastic electrophoresis separation chip are described.
    Type: Grant
    Filed: June 7, 2018
    Date of Patent: July 6, 2021
    Inventors: Eugene Tan, Cheuk Wai Kan, Heung Chuan Lam
  • Patent number: 11036779
    Abstract: Data analytics systems and methods are disclosed herein. A parser can parse reference data from various data sources to store in a data structure. An uploader can receive study data designated by a researcher and store the study data in the data structure. A matcher can compare analyte nameset data in the study data with analyte nameset data from the reference data to generate one or more links each correlating an instance of an analyte in the study data with an instance of that analyte in the reference data. Library overlays each include one or more modules to access reference data to generate organized associations of reference data. A calculation engine can receive a selection of one or more library overlay(s) and manipulate the reference data and study data according to the organized associations of the selected library overlay(s) to generate configured data stored in a collection of data caches for presentation to a researcher via a user interface.
    Type: Grant
    Filed: April 23, 2019
    Date of Patent: June 15, 2021
    Assignee: Verso Biosciences, Inc.
    Inventors: Bruce W. Yu, Chi-Ming J. Chien, Jeffrey R. Wong, Steven M. Watkins
  • Patent number: 11002657
    Abstract: An aerolysin nanopore or a nanotube is used for the electrical detection of peptides, proteins separated by at least one amino acid and other macromolecules such as polysaccharides or synthetic or natural polymers present in a preparation where said nanopore or nanotube is inserted into a lipid membrane which is subjected to a difference in potential greater than ?160 mV, in a reaction medium having an alkali metal halide electrolyte solution with a concentration of less than 6M and at a temperature of less than 40° C., and where said use is intended to differentiate said peptides, proteins and other molecules according to their length and their mass. Application to the sequencing of peptides and other molecules to differentiate them according to their length and mass with an amino acid-level or monomer-level resolution and to medical diagnosis.
    Type: Grant
    Filed: June 26, 2017
    Date of Patent: May 11, 2021
    Inventors: Juan Pelta, Abdelghani Oukhaled, Philippe Manivet, Fabien Piguet, Hadjer Ouldali, Zuzana Krupova, Pierre Defrenaix
  • Patent number: 10989685
    Abstract: A method of sensing a biological sample includes introducing a fluid containing the biological sample through a first opening in a substrate. The method further includes passing the fluid from the first opening to a first cavity through at least one microfluidic channel. The method further includes repelling the biological sample from a first surface of the first cavity using a first surface modification layer. The method further includes attracting the biological sample to a sensing device using a plurality of modified surface patterns, wherein a first modified surface pattern of the plurality of modified surface patterns has different surface properties from a second modified surface pattern of the plurality of modified surface patterns. The method further includes outputting the fluid through a second opening in the substrate.
    Type: Grant
    Filed: March 6, 2018
    Date of Patent: April 27, 2021
    Inventors: Yi-Shao Liu, Chun-Wen Cheng, Chun-Ren Cheng
  • Patent number: 10933417
    Abstract: A portable system for extracting, optionally amplifying, and detecting nucleic acids or proteins using a compact integrated chip in combination with a mobile device system for analyzing detected signals, and comparing and distributing the results via a wireless network. Related systems and methods are provided.
    Type: Grant
    Filed: March 10, 2014
    Date of Patent: March 2, 2021
    Assignee: Nanobiosym, Inc.
    Inventor: Anita Goel
  • Patent number: 10919183
    Abstract: The present invention relates generally to a method that is used to create three-dimensional synthetic vascular networks. Micromachining and molding techniques are used to create a template in a shape that mimics a biological network. Cellular material can be seeded around the template or a space created by the template and grown into an engineered tissue-construct.
    Type: Grant
    Filed: June 25, 2015
    Date of Patent: February 16, 2021
    Assignees: CARNEGIE MELLON UNIVERSITY, a Pennsylvania Non-Profit Corporation, University of Pittsburgh—Of the Commonwealth System of Higher Education
    Inventors: Philip R. LeDuc, O. Burak Ozdoganlar, Mary E. Wilson, Emrullah Korkmaz, Yadong Wang, Donna Beer-Stolz
  • Patent number: 10882041
    Abstract: A portable system for extracting, optionally amplifying, and detecting nucleic acids or proteins using a compact integrated chip in combination with a mobile device system for analyzing detected signals, and comparing and distributing the results via a wireless network. Related systems and methods are provided.
    Type: Grant
    Filed: March 10, 2014
    Date of Patent: January 5, 2021
    Assignee: Nanobiosym, Inc.
    Inventor: Anita Goel
  • Patent number: 10866212
    Abstract: An analytical tool for use in analysis of a sample is provided. The analytical tool includes a first unit and a second unit. The first unit has an analysis portion where analysis is performed. The second unit, configured to be coupled with the first unit, has a liquid reservoir for confining a particular liquid used for the analysis of the sample. When the first unit and the second unit are coupled, a fluid passage for conducting the particular liquid from the liquid reservoir into the first unit is formed by only a part of the first unit, or only a part of the second unit, or a combination of respective parts of the first unit and the second unit.
    Type: Grant
    Filed: February 8, 2018
    Date of Patent: December 15, 2020
    Assignee: ARKRAY, Inc.
    Inventor: Naotsugu Onuma
  • Patent number: 10821445
    Abstract: A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.
    Type: Grant
    Filed: May 2, 2016
    Date of Patent: November 3, 2020
    Assignee: ANDE Corporation
    Inventors: Richard F. Selden, Eugene Tan
  • Patent number: 10775345
    Abstract: An analytical method and an analytical system capable of more accurate analysis, in which a sample is analyzed by a capillary electrophoresis technique in which a voltage is applied to a sample solution introduced to a micro flow path, a separation analysis is performed for a component contained in the sample solution, and an optically measured value corresponding to an elapsed time after starting a measurement is measured. The analytical method comprises: a process of determining an interface arrival time point, based on the optically measured value when an interface between the sample solution and a migration liquid reaches a predetermined measurement position in the micro flow path; and a process of identifying the component contained in the sample solution using the optically measured value at the elapsed time after the interface arrival time point.
    Type: Grant
    Filed: October 25, 2017
    Date of Patent: September 15, 2020
    Assignee: ARKRAY, Inc.
    Inventor: Masao Kawano
  • Patent number: 10775344
    Abstract: There is provided a method for distributing power in an electrophoresis system from a power supply to each channel of an interfaced cassette connected to a pedestal located on the electrophoresis system, the method comprising: receiving a power signal from the power supply at the pedestal; receiving at least one externally generated control signal; and, modulating the power signal at a processor electrically coupled to the pedestal in dependence upon the control signal to generate a modulated power signal defined for each said cassette channel of the pedestal.
    Type: Grant
    Filed: February 24, 2015
    Date of Patent: September 15, 2020
    Assignee: Coastal Genomics Inc.
    Inventors: Jared Slobodan, Darren Cheng, Kevin Baillie, Andrew Nobles, Matthew Nesbitt
  • Patent number: 10767149
    Abstract: The present subject matter provides a microfluidic device that enables the precise and repeatable three dimensional and compartmentalized coculture of muscle cells and neuronal cells. Related apparatus, systems, techniques, and articles are also described.
    Type: Grant
    Filed: June 13, 2017
    Date of Patent: September 8, 2020
    Assignee: Massachusetts Institute of Technology
    Inventors: Roger Dale Kamm, Sebastien G M Uzel
  • Patent number: 10705046
    Abstract: Provided is an electrophoresis device that, by electrophoresis, feeds a sample into capillaries and optically detects the sample, the electrophoresis device being provided with capillaries, a capillary head provided at the distal end of the capillaries, a phoretic medium-filled container used for electrophoresis and filled with a phoretic medium, a guide member that covers the side surface of the phoretic medium-filled container, a seal member that seals from below the phoretic medium filled in the phoretic medium-filled container, and a plunger that presses the seal member.
    Type: Grant
    Filed: March 27, 2015
    Date of Patent: July 7, 2020
    Assignee: Hitachi High-Tech Corporation
    Inventors: Ryusuke Kimura, Motohiro Yamazaki, Hitoshi Miyata, Hiroyuki Higashino
  • Patent number: 10416115
    Abstract: A method of coating the inside wall of a capillary with a polymeric material for capillary electrophoresis is disclosed. The method can include introducing a catalyst-free solution of a monomer and initiator, wherein the monomer is present in about 1-10% (w/v) and the initiator is present in 0.1-1% (w/v), into a capillary and thermally initiating polymerization of the monomer thereby providing a capillary comprising an internal polymeric coating for separating, identifying, and quantifying components of an analyte.
    Type: Grant
    Filed: June 20, 2016
    Date of Patent: September 17, 2019
    Assignee: University of Nortre Dame du Lac
    Inventors: Norman Dovichi, Guijie Zhu, Liangliang Sun
  • Patent number: 10378663
    Abstract: A valve for regulating fluid flow includes a housing base defining a lower cavity and comprising a pinch structure within the lower cavity, a gas inlet providing external access to the lower cavity, a base fluid inlet, and a base fluid outlet. A housing cover defines an upper cavity and comprises a cover fluid inlet and a cover fluid outlet. The cover fluid inlet is in fluidic communication with the base fluid outlet between the upper cavity and the lower cavity, and the cover fluid outlet provides external access from the upper cavity. A diaphragm is disposed between the housing base and the housing cover. A pinch plate is disposed in the lower cavity and comprises a pinch point disposed opposite the pinch structure. A pinch tube is in fluidic communication between the base fluid inlet and the base fluid outlet in the lower cavity.
    Type: Grant
    Filed: October 1, 2018
    Date of Patent: August 13, 2019
    Inventor: Jonathan Schultz
  • Patent number: 10159977
    Abstract: The present invention generally pertains to a system for performing droplet inflation, and methods and kits comprising the same. The system comprises at least one microfluidic channel comprising one or more droplets flowing therein, one or more fluid reservoirs, one or more inflators, one or more inflator nozzles, and at least one mechanism for disrupting an interface between a droplet and a fluid. The present invention provides for the inflation of a relatively controlled volume of fluid into a droplet resulting in an increase in the volume of the droplet relative to its volume prior to inflation and, accordingly, dilution of the concentration of species, if any, previously present and emulsified in the droplet.
    Type: Grant
    Filed: January 20, 2017
    Date of Patent: December 25, 2018
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Tony Hung, Adnan Esmail, Sepehr Kiani
  • Patent number: 10018587
    Abstract: The present invention provides a method for determining whether or not a capillary filled with an electrophoretic medium is suitably used for electrophoresis before electrophoresis is performed using the analytes. The method comprises (a) applying an alternating-current voltage between a first electrode which is in contact with a first electrolyte solution in which one end of the capillary is immersed and a second electrode which is in contact with a second electrolyte solution in which the other end of the capillary is immersed to measure an electric conductivity of the electrophoresis medium with which an inside of the capillary is filled; and (b) determining that the capillary filled with the electrophoresis medium fails to be used suitably for the electrophoresis, when the electric conductivity is more than 4.2 mS/cm.
    Type: Grant
    Filed: September 29, 2016
    Date of Patent: July 10, 2018
    Assignee: Panasonic Intellectual Property Management Co., Ltd.
    Inventors: Mari Onodera, Hidenobu Yaku
  • Patent number: 9997344
    Abstract: The present disclosure describes devices and methods capable of generating multi-phase emulsions, including double emulsion droplets in a gas phase. The present disclosure also describes interfaces for coupling a multi-phase emulsion droplet source to an analytical instrument such as a mass spectrometer. The present disclosure further describes methods, systems, and apparatuses for using the devices and interfaces described to perform analysis, including mass spectrometry. The present disclosure also describes methods, systems, and apparatuses for generating and using multi-phase emulsions to perform analysis.
    Type: Grant
    Filed: May 30, 2014
    Date of Patent: June 12, 2018
    Assignee: University of Washington through its Center for Commercialization
    Inventors: Daniel T. Chiu, Dingsheng Liu
  • Patent number: 9981263
    Abstract: A flow control apparatus for facilitating treatment of a fluid containing a sample for analysis, includes: a body having spaced ends and defining a fluid flow passage arrangement that extends between the ends. The fluid flow passage arrangement includes two flow paths that are configured in parallel, merge within the body at least once, and respectively contain a one-way check valve and a medium selected to treat or modify sample-containing fluid flowing therethrough.
    Type: Grant
    Filed: December 24, 2013
    Date of Patent: May 29, 2018
    Assignee: EPREP PTY LTD
    Inventors: Ernest Frederick Dawes, Peter Alexander Dawes, Reno Cerra, Andrew Minett
  • Patent number: 9951459
    Abstract: A system for monitoring sanitation agents in a laundry machine comprises a plurality of channels. Each channel has an input port, an output port, and a glass tube. Each input port and output port is adapted to be fluidly coupled to the laundry machine. The system includes a monitoring unit associated with each of the glass tubes. Each monitoring unit includes a bubble detector and a flow rate measurement unit. A beeper is configured to generate an indication of an alarm condition based on a determination made by any one of the monitoring units. The system has a display screen, a memory storing computer-readable instructions, and a controller communicatively coupled to each of the monitoring units. The controller is configured to execute the instructions to wirelessly communicate to a web page a volume of a sanitation agent that has passed through each of the glass tubes.
    Type: Grant
    Filed: May 22, 2017
    Date of Patent: April 24, 2018
    Assignees: TCD Parts, Inc., Brightwell Dispensers Ltd
    Inventors: Mark B. Fisher, Joel Gross
  • Patent number: 9919317
    Abstract: The present invention relates to a process for the extraction of analyte compounds from a sample comprising one or more analytes in a donor phase into an acceptor phase, comprising the steps of: a) providing an electrically conductive donor phase comprising the compounds in a first electrically conductive solvent or solvent blend, and an electrode arranged in electrically conductive contact with the donor phase, b) providing an electrically conductive acceptor phase in electrically conductive contact with a second electrode; and c) providing an insulator phase in fluid communication with at least one of the donor phase and the acceptor phase, wherein the insulator phase is immiscible with the donor phase and/or the acceptor phase, and d) (d) applying an electrical field between the first and the second electrode.
    Type: Grant
    Filed: April 19, 2013
    Date of Patent: March 20, 2018
    Assignee: Universiteit Leiden
    Inventors: Robert-Jan Raterink, Peter Lindenburg, Thomas Hankemeier
  • Patent number: 9766205
    Abstract: An apparatus (1) for the measurement of a concentration of a charged species in a sample (10) is disclosed. The sample (10) comprises a plurality of types of charged species and at least one insoluble component. The apparatus (1) comprises a first circuit with a voltage control device (54) connectable to two first electrodes (30, 30?) arranged along a channel (12) holding the sample (10) and a second circuit with a conductivity detection device (55) connectable to two second electrodes (5, 5?) arranged in the channel (12). The first circuit and the second circuit are dc and ac electrically isolated from each other.
    Type: Grant
    Filed: May 19, 2014
    Date of Patent: September 19, 2017
    Assignee: CE-MATE B.V.
    Inventors: Steven S. Staal, Jan Floris, Stefan O. Lenk
  • Patent number: 9757698
    Abstract: The present invention generally relates to systems and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet contained within a fluidic channel, or a fluid may be injected into a fluidic channel to create a droplet. In some embodiments, electrodes may be used to apply an electric field to one or more fluidic channels, e.g., proximate an intersection of at least two fluidic channels. For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc.
    Type: Grant
    Filed: June 25, 2010
    Date of Patent: September 12, 2017
    Assignee: President and Fellows of Harvard College
    Inventors: David A. Weitz, Adam R. Abate, Tony Hung, Pascaline Mary
  • Patent number: 9597644
    Abstract: Provided herein is a method for analyzing the affect of at least one agent on a cell or cellular component using an analysis system. The method includes wrapping a droplet in an immiscible carrier fluid using a droplet generator wherein the wrapped droplet includes at least one cell or cellular component and at least one agent. The wrapped droplet then flows through the analysis system using a siphoning effect. The droplet is then analyzed to determine effect of the agent on the cell or cellular component.
    Type: Grant
    Filed: March 26, 2010
    Date of Patent: March 21, 2017
    Inventors: Mark Davies, Tara Dalton
  • Patent number: 9410925
    Abstract: The present invention relates to a plastic capillary tube for capillary electrophoresis, in which the plastic capillary tube has an inlet opening and an outlet opening and, furthermore, has at least one hole in the capillary tube wall and the diameter of the hole on the inside of the capillary tube wall dL(innen) lies in the range from 0.5 ?m to 30 ?m.
    Type: Grant
    Filed: September 23, 2011
    Date of Patent: August 9, 2016
    Inventors: Dominik Mueller, Karsten Pinkwart, Jens Tuebke
  • Patent number: 9285340
    Abstract: According to one embodiment, a method includes co-feeding fluids comprising a leading electrolyte, a trailing electrolyte, and at least one of DNA and RNA to a channel, and applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. In another embodiment, a method includes co-feeding fluids to a channel. The fluids include a leading electrolyte, a trailing electrolyte, biological objects, at least one of DNA and RNA, and a spacer electrolyte having an electrophoretic mobility that is between an electrophoretic mobility of at least some of the biological objects and an electrophoretic mobility of the at least one of the DNA and the RNA. The method also includes applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. Other methods of isotachophoresis are disclosed in addition to these.
    Type: Grant
    Filed: August 17, 2009
    Date of Patent: March 15, 2016
    Inventors: Byoungsok Jung, Kevin Ness, Klint A. Rose
  • Patent number: 9267918
    Abstract: A flow cell for a microfluidic device can include a chamber, first microfluidic entry and exit channels, second microfluidic entry and exit channels, a first electrode, and a second electrode. A microfluidic device can include a microfluidic channel, a laser to excite fluorescent material, and a detector to detect fluorescence emission. Methods of merging a droplet from an emulsion in to a second stream of fluid and of detecting a content of a droplet in a stream are further disclosed.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: February 23, 2016
    Assignees: Cambridge Enterprise Limited, Sphere Fluidics Limited, SDIX, LLC
    Inventors: Tony R. Joaquim, Lewis Joe Stafford, Ross Stewart Chambers, Xin Liu, Clive Adrian Smith, Xin Li, Graeme Whyte
  • Patent number: 9255905
    Abstract: Methods, systems and devices that allow independently applied pressures to a BGE reservoir and a sample reservoir for pressure-driven injection that can inject a discrete sample plug into a separation channel that does not require voltage applied to the sample reservoir and can allow for in-channel focusing methods to be used. The methods, systems and devices are particularly suitable for use with a mass spectrometer.
    Type: Grant
    Filed: May 11, 2015
    Date of Patent: February 9, 2016
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: John Scott Mellors, Erin Anne Redman, John Michael Ramsey
  • Patent number: 9180652
    Abstract: A microfluidic chip for a microfluidic system includes a PDMS substrate having a first thickness, at least one microfluidic pathway in the substrate, a coating along the microfluidic pathway, and a glass layer having a second thickness on the substrate and above the microfluidic pathway, wherein the coating contains an optically transparent material, and the first thickness is greater than the second thickness. The coating includes cyanoacrylates, an UV curable epoxy adhesive, a gel epoxy or epoxy under trade name of EPO-TEK OG175, MasterBond EP30LV-1 or Locite 0151.
    Type: Grant
    Filed: September 16, 2013
    Date of Patent: November 10, 2015
    Assignee: FluxErgy, LLC
    Inventors: Tej Patel, Ryan Revilla, Matthew D'Ooge
  • Patent number: 9101932
    Abstract: Microchannel chip (100) has film (120) adhered on the second surface of base (110) having a through-hole (111), and conductive layer (130). Film (120) has hole (121) and covers a second opening of the through-hole (111). Conductive layer (130) is integrally formed so as to extend from a portion of a bottom of hole (121) of base (110) to a portion of an inner surface of through-hole (111). Upon comparison with a microchannel chip in the related art, microchannel chip (100) is further reduced in size, has excellent handleability and is easy to be manufactured.
    Type: Grant
    Filed: September 19, 2013
    Date of Patent: August 11, 2015
    Assignee: ENPLAS Corporation
    Inventor: Koichi Ono
  • Patent number: 9073332
    Abstract: An inkjet recording apparatus, including an ink cartridge, an ink cartridge container, a tray with a supporting surface, and a slider member, is provided. The slider member is slidable on the supporting surface along a first direction, which is an orientation from a front side toward a rear side of the inkjet recording apparatus, or a second direction, which is an orientation orthogonal to the first direction. The ink cartridge container includes a container compartment to contain the ink cartridge in a position on at least one of an upstream side and a downstream side of the slider member with regard to the second direction, and a bridging section connected with the container compartment and placed on an upstream side of the slider member with regard to the first direction in a position to spread over the slider member with regard to the second direction.
    Type: Grant
    Filed: September 30, 2014
    Date of Patent: July 7, 2015
    Inventor: Yasuo Nishikawa
  • Publication number: 20150136604
    Abstract: This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.
    Type: Application
    Filed: September 29, 2014
    Publication date: May 21, 2015
    Inventors: William D. NIELSEN, Richard J. BELCINSKI, Gregory BOGDAN, David EBERHART, Omar EL-SISSI, Stevan B. JOVANOVICH, Michael RECKNOR, Ezra VAN GELDER, David W. WYRICK
  • Patent number: 9034161
    Abstract: The present invention provides methods, devices, and kits for separating and selecting top sperm from a sperm sample of a subject. In one aspect, for example, such a method can include removing a portion of negatively charged protein from sperm in the sperm sample, immobilizing the sperm, electrophoretically separating the sperm, and selecting mature sperm based on electromotility properties.
    Type: Grant
    Filed: August 23, 2013
    Date of Patent: May 19, 2015
    Inventors: Douglas T. Carrell, Luke Simon
  • Publication number: 20150060276
    Abstract: There is provided a nanopore system including a nanopore in a solid state membrane. A first reservoir is in fluidic connection with the nanopore, the first reservoir being configured to provide, to the nanopore, nucleic acid molecules in an electrolytic solution. A second reservoir is in fluidic connection with the nanopore, with the nanopore membrane separating the first and second reservoirs. A pressure source is connected to the first reservoir to apply an external pressure to the first reservoir to cause nanopore translocation of nucleic acid molecules in the solution in the first reservoir. A voltage source is connected between the second and first reservoirs, across the nanopore, with a voltage bias polarity that applies an electric field counter to the externally applied pressure. Force of the externally applied pressure is greater than force of the electric field during nanopore translocation by the nucleic acid molecules.
    Type: Application
    Filed: September 12, 2014
    Publication date: March 5, 2015
    Inventors: Jene A. Golovchenko, Bo Lu, David P. Hoogerheide, Dapeng Yu, Qing Zhao
  • Publication number: 20150060277
    Abstract: There is provided a nanopore system including a nanopore in a support structure. A first reservoir is in fluidic connection with the nanopore and a second reservoir is in fluidic connection with the nanopore. The support structure separates the first and second reservoirs. A pressure source is connected to one of the first and second reservoirs to apply an external pressure to one of the first and second reservoirs. A voltage source is connected between the first and second reservoirs to apply a voltage bias between the first and second reservoirs, across the nanopore. This system enables a method for analysis of species in solution, wherein there is provided to the nanopore a fluidic solution that includes a species for translocation through the nanopore, with an external pressure applied to the species in fluidic solution and a voltage bias applied across the nanopore.
    Type: Application
    Filed: September 12, 2014
    Publication date: March 5, 2015
    Inventors: Jene A. Golovchenko, Bo Lu, David P. Hoogerheide, Dapeng Yu, Qing Zhao
  • Patent number: 8968678
    Abstract: An apparatus and methods for concentrating samples for application to microfluidic devices are disclosed. The methods involve electrophoresing charged molecules from a high volume sample into a smaller volume. The analyte of interest can be a charged molecule or can be modified to be charged using, for example, one or more ionic moieties.
    Type: Grant
    Filed: June 28, 2012
    Date of Patent: March 3, 2015
    Assignee: Headway Technologies, Inc.
    Inventor: Celine Hu
  • Publication number: 20150047979
    Abstract: A method of fluid testing includes pressurizing a fluid testing system, disposed at a subterranean location under high pressure compared to a surface pressure, to achieve a desired pressure differential between the high pressure and an internal pressure of the fluid testing system. The fluid testing system includes a capillary electrophoresis system and one or more test fluid reservoirs. The method also includes directing test fluid from the one or more test fluid reservoirs into capillaries to condition the capillaries. The method further includes directing sample fluid into the capillaries for testing while at the subterranean location.
    Type: Application
    Filed: August 15, 2013
    Publication date: February 19, 2015
    Applicant: Schlumberger Technology Corporation
    Inventors: Sharath Chandra Mahavadi, Geza Horvath Szabo, Simon Ivar Andersen
  • Patent number: 8871072
    Abstract: Flow step focusing isolates and concentrates a molecule of interest by flowing a liquid comprising a molecule of interest through a main channel having an inlet and an outlet with application of a first pressure at the inlet; applying a voltage along the channel during the flowing, wherein the voltage is configured to have a polarity such that it drives the molecule of interest in a direction opposite the flow of the liquid; controlling the first pressure and/or the voltage in a manner so as to trap and concentrate the molecule of interest in a region of the main channel; and removing the concentrated molecule of interest from the channel by recovering a portion of the liquid from a side channel diverging from the main channel, wherein the side channel is maintained at a pressure lower than the first pressure. Also disclosed is an apparatus for such.
    Type: Grant
    Filed: September 24, 2012
    Date of Patent: October 28, 2014
    Assignee: The United States of America, as represented by the Secretary of the Navy
    Inventor: Peter B. Howell, Jr.
  • Patent number: 8841116
    Abstract: Methods and microfluidic circuitry for inline injection of nucleic acids for capillary electrophoresis analysis are provided. According to various embodiments, microfabricated structures including affinity-based capture matrixes inline with separation channels are provided. The affinity-based capture matrixes provide inline sample plug formation and injection into a capillary electrophoresis channel. Also provided are methods and apparatuses for a microbead-based inline injection system for DNA sequencing.
    Type: Grant
    Filed: October 25, 2007
    Date of Patent: September 23, 2014
    Assignee: The Regents of the University of California
    Inventors: Richard A. Mathies, Robert Blazej, Palani Kumaresan
  • Publication number: 20140262784
    Abstract: The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.
    Type: Application
    Filed: May 25, 2012
    Publication date: September 18, 2014
    Inventors: James Clarke, James White, John Milton, Clive Brown