With Injection Patents (Class 204/453)
  • Patent number: 10821445
    Abstract: A biochip for the integration of all steps in a complex process from the insertion of a sample to the generation of a result, performed without operator intervention includes microfluidic and macrofluidic features that are acted on by instrument subsystems in a series of scripted processing steps. Methods for fabricating these complex biochips of high feature density by injection molding are also provided.
    Type: Grant
    Filed: May 2, 2016
    Date of Patent: November 3, 2020
    Assignee: ANDE Corporation
    Inventors: Richard F. Selden, Eugene Tan
  • Patent number: 10775345
    Abstract: An analytical method and an analytical system capable of more accurate analysis, in which a sample is analyzed by a capillary electrophoresis technique in which a voltage is applied to a sample solution introduced to a micro flow path, a separation analysis is performed for a component contained in the sample solution, and an optically measured value corresponding to an elapsed time after starting a measurement is measured. The analytical method comprises: a process of determining an interface arrival time point, based on the optically measured value when an interface between the sample solution and a migration liquid reaches a predetermined measurement position in the micro flow path; and a process of identifying the component contained in the sample solution using the optically measured value at the elapsed time after the interface arrival time point.
    Type: Grant
    Filed: October 25, 2017
    Date of Patent: September 15, 2020
    Assignee: ARKRAY, Inc.
    Inventor: Masao Kawano
  • Patent number: 10775344
    Abstract: There is provided a method for distributing power in an electrophoresis system from a power supply to each channel of an interfaced cassette connected to a pedestal located on the electrophoresis system, the method comprising: receiving a power signal from the power supply at the pedestal; receiving at least one externally generated control signal; and, modulating the power signal at a processor electrically coupled to the pedestal in dependence upon the control signal to generate a modulated power signal defined for each said cassette channel of the pedestal.
    Type: Grant
    Filed: February 24, 2015
    Date of Patent: September 15, 2020
    Assignee: Coastal Genomics Inc.
    Inventors: Jared Slobodan, Darren Cheng, Kevin Baillie, Andrew Nobles, Matthew Nesbitt
  • Patent number: 10767149
    Abstract: The present subject matter provides a microfluidic device that enables the precise and repeatable three dimensional and compartmentalized coculture of muscle cells and neuronal cells. Related apparatus, systems, techniques, and articles are also described.
    Type: Grant
    Filed: June 13, 2017
    Date of Patent: September 8, 2020
    Assignee: Massachusetts Institute of Technology
    Inventors: Roger Dale Kamm, Sebastien G M Uzel
  • Patent number: 10705046
    Abstract: Provided is an electrophoresis device that, by electrophoresis, feeds a sample into capillaries and optically detects the sample, the electrophoresis device being provided with capillaries, a capillary head provided at the distal end of the capillaries, a phoretic medium-filled container used for electrophoresis and filled with a phoretic medium, a guide member that covers the side surface of the phoretic medium-filled container, a seal member that seals from below the phoretic medium filled in the phoretic medium-filled container, and a plunger that presses the seal member.
    Type: Grant
    Filed: March 27, 2015
    Date of Patent: July 7, 2020
    Assignee: Hitachi High-Tech Corporation
    Inventors: Ryusuke Kimura, Motohiro Yamazaki, Hitoshi Miyata, Hiroyuki Higashino
  • Patent number: 10416115
    Abstract: A method of coating the inside wall of a capillary with a polymeric material for capillary electrophoresis is disclosed. The method can include introducing a catalyst-free solution of a monomer and initiator, wherein the monomer is present in about 1-10% (w/v) and the initiator is present in 0.1-1% (w/v), into a capillary and thermally initiating polymerization of the monomer thereby providing a capillary comprising an internal polymeric coating for separating, identifying, and quantifying components of an analyte.
    Type: Grant
    Filed: June 20, 2016
    Date of Patent: September 17, 2019
    Assignee: University of Nortre Dame du Lac
    Inventors: Norman Dovichi, Guijie Zhu, Liangliang Sun
  • Patent number: 10378663
    Abstract: A valve for regulating fluid flow includes a housing base defining a lower cavity and comprising a pinch structure within the lower cavity, a gas inlet providing external access to the lower cavity, a base fluid inlet, and a base fluid outlet. A housing cover defines an upper cavity and comprises a cover fluid inlet and a cover fluid outlet. The cover fluid inlet is in fluidic communication with the base fluid outlet between the upper cavity and the lower cavity, and the cover fluid outlet provides external access from the upper cavity. A diaphragm is disposed between the housing base and the housing cover. A pinch plate is disposed in the lower cavity and comprises a pinch point disposed opposite the pinch structure. A pinch tube is in fluidic communication between the base fluid inlet and the base fluid outlet in the lower cavity.
    Type: Grant
    Filed: October 1, 2018
    Date of Patent: August 13, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventor: Jonathan Schultz
  • Patent number: 10159977
    Abstract: The present invention generally pertains to a system for performing droplet inflation, and methods and kits comprising the same. The system comprises at least one microfluidic channel comprising one or more droplets flowing therein, one or more fluid reservoirs, one or more inflators, one or more inflator nozzles, and at least one mechanism for disrupting an interface between a droplet and a fluid. The present invention provides for the inflation of a relatively controlled volume of fluid into a droplet resulting in an increase in the volume of the droplet relative to its volume prior to inflation and, accordingly, dilution of the concentration of species, if any, previously present and emulsified in the droplet.
    Type: Grant
    Filed: January 20, 2017
    Date of Patent: December 25, 2018
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Tony Hung, Adnan Esmail, Sepehr Kiani
  • Patent number: 10018587
    Abstract: The present invention provides a method for determining whether or not a capillary filled with an electrophoretic medium is suitably used for electrophoresis before electrophoresis is performed using the analytes. The method comprises (a) applying an alternating-current voltage between a first electrode which is in contact with a first electrolyte solution in which one end of the capillary is immersed and a second electrode which is in contact with a second electrolyte solution in which the other end of the capillary is immersed to measure an electric conductivity of the electrophoresis medium with which an inside of the capillary is filled; and (b) determining that the capillary filled with the electrophoresis medium fails to be used suitably for the electrophoresis, when the electric conductivity is more than 4.2 mS/cm.
    Type: Grant
    Filed: September 29, 2016
    Date of Patent: July 10, 2018
    Assignee: Panasonic Intellectual Property Management Co., Ltd.
    Inventors: Mari Onodera, Hidenobu Yaku
  • Patent number: 9997344
    Abstract: The present disclosure describes devices and methods capable of generating multi-phase emulsions, including double emulsion droplets in a gas phase. The present disclosure also describes interfaces for coupling a multi-phase emulsion droplet source to an analytical instrument such as a mass spectrometer. The present disclosure further describes methods, systems, and apparatuses for using the devices and interfaces described to perform analysis, including mass spectrometry. The present disclosure also describes methods, systems, and apparatuses for generating and using multi-phase emulsions to perform analysis.
    Type: Grant
    Filed: May 30, 2014
    Date of Patent: June 12, 2018
    Assignee: University of Washington through its Center for Commercialization
    Inventors: Daniel T. Chiu, Dingsheng Liu
  • Patent number: 9981263
    Abstract: A flow control apparatus for facilitating treatment of a fluid containing a sample for analysis, includes: a body having spaced ends and defining a fluid flow passage arrangement that extends between the ends. The fluid flow passage arrangement includes two flow paths that are configured in parallel, merge within the body at least once, and respectively contain a one-way check valve and a medium selected to treat or modify sample-containing fluid flowing therethrough.
    Type: Grant
    Filed: December 24, 2013
    Date of Patent: May 29, 2018
    Assignee: EPREP PTY LTD
    Inventors: Ernest Frederick Dawes, Peter Alexander Dawes, Reno Cerra, Andrew Minett
  • Patent number: 9951459
    Abstract: A system for monitoring sanitation agents in a laundry machine comprises a plurality of channels. Each channel has an input port, an output port, and a glass tube. Each input port and output port is adapted to be fluidly coupled to the laundry machine. The system includes a monitoring unit associated with each of the glass tubes. Each monitoring unit includes a bubble detector and a flow rate measurement unit. A beeper is configured to generate an indication of an alarm condition based on a determination made by any one of the monitoring units. The system has a display screen, a memory storing computer-readable instructions, and a controller communicatively coupled to each of the monitoring units. The controller is configured to execute the instructions to wirelessly communicate to a web page a volume of a sanitation agent that has passed through each of the glass tubes.
    Type: Grant
    Filed: May 22, 2017
    Date of Patent: April 24, 2018
    Assignees: TCD Parts, Inc., Brightwell Dispensers Ltd
    Inventors: Mark B. Fisher, Joel Gross
  • Patent number: 9919317
    Abstract: The present invention relates to a process for the extraction of analyte compounds from a sample comprising one or more analytes in a donor phase into an acceptor phase, comprising the steps of: a) providing an electrically conductive donor phase comprising the compounds in a first electrically conductive solvent or solvent blend, and an electrode arranged in electrically conductive contact with the donor phase, b) providing an electrically conductive acceptor phase in electrically conductive contact with a second electrode; and c) providing an insulator phase in fluid communication with at least one of the donor phase and the acceptor phase, wherein the insulator phase is immiscible with the donor phase and/or the acceptor phase, and d) (d) applying an electrical field between the first and the second electrode.
    Type: Grant
    Filed: April 19, 2013
    Date of Patent: March 20, 2018
    Assignee: Universiteit Leiden
    Inventors: Robert-Jan Raterink, Peter Lindenburg, Thomas Hankemeier
  • Patent number: 9766205
    Abstract: An apparatus (1) for the measurement of a concentration of a charged species in a sample (10) is disclosed. The sample (10) comprises a plurality of types of charged species and at least one insoluble component. The apparatus (1) comprises a first circuit with a voltage control device (54) connectable to two first electrodes (30, 30?) arranged along a channel (12) holding the sample (10) and a second circuit with a conductivity detection device (55) connectable to two second electrodes (5, 5?) arranged in the channel (12). The first circuit and the second circuit are dc and ac electrically isolated from each other.
    Type: Grant
    Filed: May 19, 2014
    Date of Patent: September 19, 2017
    Assignee: CE-MATE B.V.
    Inventors: Steven S. Staal, Jan Floris, Stefan O. Lenk
  • Patent number: 9757698
    Abstract: The present invention generally relates to systems and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet contained within a fluidic channel, or a fluid may be injected into a fluidic channel to create a droplet. In some embodiments, electrodes may be used to apply an electric field to one or more fluidic channels, e.g., proximate an intersection of at least two fluidic channels. For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc.
    Type: Grant
    Filed: June 25, 2010
    Date of Patent: September 12, 2017
    Assignee: President and Fellows of Harvard College
    Inventors: David A. Weitz, Adam R. Abate, Tony Hung, Pascaline Mary
  • Patent number: 9597644
    Abstract: Provided herein is a method for analyzing the affect of at least one agent on a cell or cellular component using an analysis system. The method includes wrapping a droplet in an immiscible carrier fluid using a droplet generator wherein the wrapped droplet includes at least one cell or cellular component and at least one agent. The wrapped droplet then flows through the analysis system using a siphoning effect. The droplet is then analyzed to determine effect of the agent on the cell or cellular component.
    Type: Grant
    Filed: March 26, 2010
    Date of Patent: March 21, 2017
    Assignee: STOKES BIO LIMITED
    Inventors: Mark Davies, Tara Dalton
  • Patent number: 9410925
    Abstract: The present invention relates to a plastic capillary tube for capillary electrophoresis, in which the plastic capillary tube has an inlet opening and an outlet opening and, furthermore, has at least one hole in the capillary tube wall and the diameter of the hole on the inside of the capillary tube wall dL(innen) lies in the range from 0.5 ?m to 30 ?m.
    Type: Grant
    Filed: September 23, 2011
    Date of Patent: August 9, 2016
    Assignee: FRAUNHOFER-GESELLSCHAFT ZUR FORDERUNG DER ANGEWANDTEN FORSCHUNG E.V.
    Inventors: Dominik Mueller, Karsten Pinkwart, Jens Tuebke
  • Patent number: 9285340
    Abstract: According to one embodiment, a method includes co-feeding fluids comprising a leading electrolyte, a trailing electrolyte, and at least one of DNA and RNA to a channel, and applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. In another embodiment, a method includes co-feeding fluids to a channel. The fluids include a leading electrolyte, a trailing electrolyte, biological objects, at least one of DNA and RNA, and a spacer electrolyte having an electrophoretic mobility that is between an electrophoretic mobility of at least some of the biological objects and an electrophoretic mobility of the at least one of the DNA and the RNA. The method also includes applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. Other methods of isotachophoresis are disclosed in addition to these.
    Type: Grant
    Filed: August 17, 2009
    Date of Patent: March 15, 2016
    Inventors: Byoungsok Jung, Kevin Ness, Klint A. Rose
  • Patent number: 9267918
    Abstract: A flow cell for a microfluidic device can include a chamber, first microfluidic entry and exit channels, second microfluidic entry and exit channels, a first electrode, and a second electrode. A microfluidic device can include a microfluidic channel, a laser to excite fluorescent material, and a detector to detect fluorescence emission. Methods of merging a droplet from an emulsion in to a second stream of fluid and of detecting a content of a droplet in a stream are further disclosed.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: February 23, 2016
    Assignees: Cambridge Enterprise Limited, Sphere Fluidics Limited, SDIX, LLC
    Inventors: Tony R. Joaquim, Lewis Joe Stafford, Ross Stewart Chambers, Xin Liu, Clive Adrian Smith, Xin Li, Graeme Whyte
  • Patent number: 9255905
    Abstract: Methods, systems and devices that allow independently applied pressures to a BGE reservoir and a sample reservoir for pressure-driven injection that can inject a discrete sample plug into a separation channel that does not require voltage applied to the sample reservoir and can allow for in-channel focusing methods to be used. The methods, systems and devices are particularly suitable for use with a mass spectrometer.
    Type: Grant
    Filed: May 11, 2015
    Date of Patent: February 9, 2016
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: John Scott Mellors, Erin Anne Redman, John Michael Ramsey
  • Patent number: 9180652
    Abstract: A microfluidic chip for a microfluidic system includes a PDMS substrate having a first thickness, at least one microfluidic pathway in the substrate, a coating along the microfluidic pathway, and a glass layer having a second thickness on the substrate and above the microfluidic pathway, wherein the coating contains an optically transparent material, and the first thickness is greater than the second thickness. The coating includes cyanoacrylates, an UV curable epoxy adhesive, a gel epoxy or epoxy under trade name of EPO-TEK OG175, MasterBond EP30LV-1 or Locite 0151.
    Type: Grant
    Filed: September 16, 2013
    Date of Patent: November 10, 2015
    Assignee: FluxErgy, LLC
    Inventors: Tej Patel, Ryan Revilla, Matthew D'Ooge
  • Patent number: 9101932
    Abstract: Microchannel chip (100) has film (120) adhered on the second surface of base (110) having a through-hole (111), and conductive layer (130). Film (120) has hole (121) and covers a second opening of the through-hole (111). Conductive layer (130) is integrally formed so as to extend from a portion of a bottom of hole (121) of base (110) to a portion of an inner surface of through-hole (111). Upon comparison with a microchannel chip in the related art, microchannel chip (100) is further reduced in size, has excellent handleability and is easy to be manufactured.
    Type: Grant
    Filed: September 19, 2013
    Date of Patent: August 11, 2015
    Assignee: ENPLAS Corporation
    Inventor: Koichi Ono
  • Patent number: 9073332
    Abstract: An inkjet recording apparatus, including an ink cartridge, an ink cartridge container, a tray with a supporting surface, and a slider member, is provided. The slider member is slidable on the supporting surface along a first direction, which is an orientation from a front side toward a rear side of the inkjet recording apparatus, or a second direction, which is an orientation orthogonal to the first direction. The ink cartridge container includes a container compartment to contain the ink cartridge in a position on at least one of an upstream side and a downstream side of the slider member with regard to the second direction, and a bridging section connected with the container compartment and placed on an upstream side of the slider member with regard to the first direction in a position to spread over the slider member with regard to the second direction.
    Type: Grant
    Filed: September 30, 2014
    Date of Patent: July 7, 2015
    Assignee: BROTHER KOGYO KABUSHIKI KAISHA
    Inventor: Yasuo Nishikawa
  • Publication number: 20150136604
    Abstract: This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.
    Type: Application
    Filed: September 29, 2014
    Publication date: May 21, 2015
    Inventors: William D. NIELSEN, Richard J. BELCINSKI, Gregory BOGDAN, David EBERHART, Omar EL-SISSI, Stevan B. JOVANOVICH, Michael RECKNOR, Ezra VAN GELDER, David W. WYRICK
  • Patent number: 9034161
    Abstract: The present invention provides methods, devices, and kits for separating and selecting top sperm from a sperm sample of a subject. In one aspect, for example, such a method can include removing a portion of negatively charged protein from sperm in the sperm sample, immobilizing the sperm, electrophoretically separating the sperm, and selecting mature sperm based on electromotility properties.
    Type: Grant
    Filed: August 23, 2013
    Date of Patent: May 19, 2015
    Inventors: Douglas T. Carrell, Luke Simon
  • Publication number: 20150060277
    Abstract: There is provided a nanopore system including a nanopore in a support structure. A first reservoir is in fluidic connection with the nanopore and a second reservoir is in fluidic connection with the nanopore. The support structure separates the first and second reservoirs. A pressure source is connected to one of the first and second reservoirs to apply an external pressure to one of the first and second reservoirs. A voltage source is connected between the first and second reservoirs to apply a voltage bias between the first and second reservoirs, across the nanopore. This system enables a method for analysis of species in solution, wherein there is provided to the nanopore a fluidic solution that includes a species for translocation through the nanopore, with an external pressure applied to the species in fluidic solution and a voltage bias applied across the nanopore.
    Type: Application
    Filed: September 12, 2014
    Publication date: March 5, 2015
    Applicants: PEKING UNIVERSITY, PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Jene A. Golovchenko, Bo Lu, David P. Hoogerheide, Dapeng Yu, Qing Zhao
  • Publication number: 20150060276
    Abstract: There is provided a nanopore system including a nanopore in a solid state membrane. A first reservoir is in fluidic connection with the nanopore, the first reservoir being configured to provide, to the nanopore, nucleic acid molecules in an electrolytic solution. A second reservoir is in fluidic connection with the nanopore, with the nanopore membrane separating the first and second reservoirs. A pressure source is connected to the first reservoir to apply an external pressure to the first reservoir to cause nanopore translocation of nucleic acid molecules in the solution in the first reservoir. A voltage source is connected between the second and first reservoirs, across the nanopore, with a voltage bias polarity that applies an electric field counter to the externally applied pressure. Force of the externally applied pressure is greater than force of the electric field during nanopore translocation by the nucleic acid molecules.
    Type: Application
    Filed: September 12, 2014
    Publication date: March 5, 2015
    Applicants: PEKING UNIVERSITY, PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Jene A. Golovchenko, Bo Lu, David P. Hoogerheide, Dapeng Yu, Qing Zhao
  • Patent number: 8968678
    Abstract: An apparatus and methods for concentrating samples for application to microfluidic devices are disclosed. The methods involve electrophoresing charged molecules from a high volume sample into a smaller volume. The analyte of interest can be a charged molecule or can be modified to be charged using, for example, one or more ionic moieties.
    Type: Grant
    Filed: June 28, 2012
    Date of Patent: March 3, 2015
    Assignee: Headway Technologies, Inc.
    Inventor: Celine Hu
  • Publication number: 20150047979
    Abstract: A method of fluid testing includes pressurizing a fluid testing system, disposed at a subterranean location under high pressure compared to a surface pressure, to achieve a desired pressure differential between the high pressure and an internal pressure of the fluid testing system. The fluid testing system includes a capillary electrophoresis system and one or more test fluid reservoirs. The method also includes directing test fluid from the one or more test fluid reservoirs into capillaries to condition the capillaries. The method further includes directing sample fluid into the capillaries for testing while at the subterranean location.
    Type: Application
    Filed: August 15, 2013
    Publication date: February 19, 2015
    Applicant: Schlumberger Technology Corporation
    Inventors: Sharath Chandra Mahavadi, Geza Horvath Szabo, Simon Ivar Andersen
  • Patent number: 8871072
    Abstract: Flow step focusing isolates and concentrates a molecule of interest by flowing a liquid comprising a molecule of interest through a main channel having an inlet and an outlet with application of a first pressure at the inlet; applying a voltage along the channel during the flowing, wherein the voltage is configured to have a polarity such that it drives the molecule of interest in a direction opposite the flow of the liquid; controlling the first pressure and/or the voltage in a manner so as to trap and concentrate the molecule of interest in a region of the main channel; and removing the concentrated molecule of interest from the channel by recovering a portion of the liquid from a side channel diverging from the main channel, wherein the side channel is maintained at a pressure lower than the first pressure. Also disclosed is an apparatus for such.
    Type: Grant
    Filed: September 24, 2012
    Date of Patent: October 28, 2014
    Assignee: The United States of America, as represented by the Secretary of the Navy
    Inventor: Peter B. Howell, Jr.
  • Patent number: 8841116
    Abstract: Methods and microfluidic circuitry for inline injection of nucleic acids for capillary electrophoresis analysis are provided. According to various embodiments, microfabricated structures including affinity-based capture matrixes inline with separation channels are provided. The affinity-based capture matrixes provide inline sample plug formation and injection into a capillary electrophoresis channel. Also provided are methods and apparatuses for a microbead-based inline injection system for DNA sequencing.
    Type: Grant
    Filed: October 25, 2007
    Date of Patent: September 23, 2014
    Assignee: The Regents of the University of California
    Inventors: Richard A. Mathies, Robert Blazej, Palani Kumaresan
  • Publication number: 20140262784
    Abstract: The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.
    Type: Application
    Filed: May 25, 2012
    Publication date: September 18, 2014
    Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED
    Inventors: James Clarke, James White, John Milton, Clive Brown
  • Patent number: 8834697
    Abstract: A channel (12) is formed inside a transparent substrate (11), and the two ends are respectively connected to a sample inlet (13) and a separation medium filling port (14) both open to outside, and a sample outlet (15) which is open to outside is provided on the channel (12). The portion between the sample inlet (13) and the sample outlet (15) is a separation channel portion (12a), and the portion between the sample outlet (15) and the separation medium filling port (14) is a separation medium introduction channel portion (12b). A separation medium supplier is connected to the separation medium filling port (14) and the separation medium introduction channel portion (12b) is filled with a separation medium. Then a sample is dropped to the sample inlet (13), with the sample medium supplier still connected, to introduce the sample to the separation channel portion (12a). And then, buffer liquid is poured in buffer reservoirs (16) and (17) and a migration voltage is applied to perform a migration analysis.
    Type: Grant
    Filed: April 25, 2007
    Date of Patent: September 16, 2014
    Assignee: Shimadzu Corporation
    Inventor: Toru Kaji
  • Patent number: 8828209
    Abstract: This invention discloses a highly efficient method, system and apparatus for nucleic acid analysis, including sequencing (both automated re-sequencing and de-novo sequencing). The system is capable of sequencing DNA sizes ranging from fragments to mammalian size genomes having mouse draft quality at a much reduced cost. The system comprises a massive parallel capillary electrophoretic separation using two-dimensional monolith multi-capillary arrays (2D-MMCA). Sequence identification can be performed using fluorescent or otherwise labeled dideoxynucleotide-terminated DNA extension product generated by gel matrix-, or beads-, or substrate tethered-, or otherwise immobilized colonies of single template molecules.
    Type: Grant
    Filed: June 22, 2006
    Date of Patent: September 9, 2014
    Assignee: The Research Foundation for the State University of New York
    Inventors: Vera Gorfinkel, Evgeni A. Kabotyanski, Boris Gorbovitski
  • Publication number: 20140238856
    Abstract: Devices for controlling the capture, trapping, and transport of macromolecules include at least one fluidic transport nanochannel that intersects and is in fluid communication with at least one transverse nanochannel with (shallow) regions and/or with integrated transverse electrodes that enable fine control of molecule transport dynamics and facilitates analyses of interest, e.g., molecular identification, length determination, localized (probe) mapping and the like.
    Type: Application
    Filed: February 26, 2014
    Publication date: August 28, 2014
    Applicant: The University of North Carolina at Chapel Hill
    Inventors: John Michael Ramsey, Laurent Menard
  • Patent number: 8784626
    Abstract: A detection optics configuration for bio-analysis, in which the direction of incident radiation, the axis of the separation channel, and the direction of collection of the output radiation are coplanar at the detection zone. The detection configuration incorporates ball-end optical fibers to direct incident radiation at and collection of output radiation from the detection zone. The detection optics configuration may be implemented in an improved bio-separation instrument, in particular a capillary electrophoresis instrument.
    Type: Grant
    Filed: January 28, 2011
    Date of Patent: July 22, 2014
    Assignee: Bioptic, Inc.
    Inventors: Varouj D. Amirkhanian, Shou-Kuan Tsai
  • Patent number: 8778155
    Abstract: A cartridge-based bio-separation system configured to utilize a pen shaped bio-separation cartridge that is easy to assemble and use with no moving parts and that has an integrated reagent (separation buffer) reservoir. The cartridge includes a body, defining an opening as a detection window for receiving external detection optics, at least one capillary column supported in the body, having a first end extending beyond a first end of the body, wherein the detection window exposes a section along the capillary column, to which the external optics are aligned through the detection window, and a reservoir attached to a second end of the body in fluid flow communication with a second end of the capillary column. The reservoir is structured to be coupled to an air pressure pump that pressurizes the gel reservoir to purge and fill the capillaries with buffer as the separation support medium.
    Type: Grant
    Filed: August 18, 2011
    Date of Patent: July 15, 2014
    Assignee: Bioptic, Inc.
    Inventors: Shou-Kuan Tsai, Varouj D. Amirkhanian
  • Publication number: 20140151229
    Abstract: The invention provides microfluidic devices, systems, and methods for manipulating an object within a channel of a microfluidic device using an external electrode. The device has a channel disposed within the device, the channel having no included electrodes. The channel has a wall, at least a portion of which is penetrable by an electric field generated external to the device, the wall being penetrable such that the electric field extends through the wall portion and into a region within the channel. The system includes the microfluidic device and an electrode external to and not bonded to the device. In the method, the external electrode is placed adjacent to the device and energized to generate an electric field that extends through the wall of the device and into the channel, thereby manipulating an object within the channel.
    Type: Application
    Filed: December 5, 2012
    Publication date: June 5, 2014
    Applicant: CALIPER LIFE SCIENCES, INC.
    Inventors: Joshua I. Molho, Daniel G. Stearns, I-Jane Chen, Danh Tran, Bradley W. Rice, Tobias Daniel Wheeler
  • Patent number: 8685343
    Abstract: An apparatus and methods for concentrating samples for application to microfluidic devices are disclosed. The methods involve electrophoresing charged molecules from a high volume sample into a smaller volume. The analyte of interest can be a charged molecule or can be modified to be charged using, for example, one or more ionic moieties.
    Type: Grant
    Filed: June 28, 2012
    Date of Patent: April 1, 2014
    Assignee: Headway Technologies, Inc.
    Inventor: Celine Hu
  • Publication number: 20140038080
    Abstract: There is disclosed articles for and methods of confining volatile materials in the void volume defined by crystalline void materials. In one embodiment, the hydrogen isotopes are confined inside carbon nanotubes for storage and the production of energy. There is also disclosed a method of generating various reactions by confining the volatile materials inside the crystalline void structure and releasing the confined volatile material. In this embodiment, the released volatile material may be combined with a different material to initiate or sustain a chemical, thermal, nuclear, electrical, mechanical, or biological reaction.
    Type: Application
    Filed: October 9, 2013
    Publication date: February 6, 2014
    Inventors: William K. Cooper, James F. Loan, Christopher H. Cooper
  • Publication number: 20140021050
    Abstract: Described are methods of reducing the incidence and/or magnitude of artifacts in denaturing nucleic acid capillary electrophoresis (CE). Methods and systems described serve to dismiss non-denatured DNA from the tip of the capillary after sample injection and prior to electrophoretic separation of loaded nucleic acids. Among the methods disclosed are the application of a brief reverse-polarity pulse to the capillary prior to separation but after removal of the capillary from the sample reservoir, and transiently heating the capillary to cause expansion of the separation matrix after removal of the capillary from the sample reservoir.
    Type: Application
    Filed: January 30, 2012
    Publication date: January 23, 2014
    Applicant: PRIMERADX, INC.
    Inventors: Sergey V. Voronov, Jork Nolling
  • Publication number: 20130341190
    Abstract: The invention relates to a microfabricated device and methods of using the device for analyzing and sorting polynucleotide molecules by size.
    Type: Application
    Filed: January 14, 2013
    Publication date: December 26, 2013
    Applicant: California Institute of Technology
    Inventor: California Institute of Technology
  • Publication number: 20130341189
    Abstract: A method for nucleic acid analysis including injecting a slight amount of nucleic acid sample for analysis, characterized in that most of a nucleic acid sample which is not used, excluding the slight amount of the nucleic acid sample which is used for analysis, can be obtained as a pure product which is not contaminated with a fluorescent material, and a microchip for analyzing nucleic acid which enables such method for nucleic acid analysis, are provided. The method for nucleic acid analysis may analyze at least two different nucleic acid samples in a continuous manner by sequentially injecting the at least two different nucleic acid samples.
    Type: Application
    Filed: March 7, 2013
    Publication date: December 26, 2013
    Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION
    Inventors: JONG HOON HAHN, BYOUNG JOO KWAK, HAN-OK KIM
  • Patent number: 8585973
    Abstract: In one embodiment, a method is provided for the manufacture of a nano-sensor array. A base having a sensing region is provided along with a plurality of nano-sensors. Each of the plurality of nano-sensors is formed by: forming a first nanoneedle along a surface of the base, forming a dielectric on the first nanoneedle, and forming a second nanoneedle on the dielectric layer. The first nanoneedle of each sensor has a first end adjacent to the sensing region of the base. The second nanoneedle is separated from the first nanoneedle by the dielectric and has a first end adjacent the first end of the first nanoneedle. The base is provided with a fluidic channel. The plurality of nano-sensors and the fluidic channel are configured and arranged with the first ends proximate the fluidic channel to facilitate sensing of targeted matter in the fluidic channel.
    Type: Grant
    Filed: May 27, 2011
    Date of Patent: November 19, 2013
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventor: Hesaam Esfandyarpour
  • Publication number: 20130180855
    Abstract: A capillary electrophoresis method for identification and analyzing collagen quantitatively, which is used to identify and quantify collagen in a sample, comprises the steps of: (a) dissolving a collagen-containing sample to form a sample solution; (b) preparing a capillary with an inner wall thereof having a positively-charged layer; (c) introducing the sample solution into the capillary filled with an analytical buffer solution; and (d) driving the sample solution to pass through the capillary. The method of the present invention does not need the purifying pre-treatment and cracking the collagen-containing sample but directly performs the capillary electrophoresis analysis of collagen. Therefore, the present invention can shorten the time for analyzing collagen quantitatively.
    Type: Application
    Filed: December 20, 2012
    Publication date: July 18, 2013
    Applicant: NATIONAL CHENG KUNG UNIVERSITY
    Inventor: National Cheng Kung University
  • Publication number: 20130175170
    Abstract: Embodiments of electrophoresis systems, devices, and associated methods of analysis are described herein. In one embodiment, an electrophoresis device includes a first electrode having a first polarity, a second electrode having a second polarity, and a substrate. The substrate includes a first channel having a first section with a first cross-sectional area and a second section with a second cross-sectional area. The first end is electrically coupled to the first electrode, and the second end is electrically coupled to the second electrode. The first cross-sectional area is greater than the second cross-sectional area in a first dimension and in a second dimension generally orthogonal to the first dimension.
    Type: Application
    Filed: February 10, 2012
    Publication date: July 11, 2013
    Applicant: WASHINGTON STATE UNIVERSITY RESEARCH FOUNDATION
    Inventor: Cornelius F. Ivory
  • Publication number: 20130175171
    Abstract: A device for separating and concentrating particles present in a fluid, including: a first microchannel, having at least one first aperture; and at least one second microchannel, having at least one second aperture, and an end is disclosed. The first microchannel surrounds part or all of the second microchannel at the end. The first microchannel and the second microchannel are connected, at the end, by at least one nanochannel, the nanochannel(s) forming a restriction between the first microchannel and the second microchannel. A cap bounds the first microchannel, the second microchannel and the nanochannel at the end. The first microchannel and the second microchannel are made in a first substrate. The first aperture and the second aperture open into a same face of this substrate. The device may be used for separating and concentrating particles of biological samples, such as viruses, DNA or synthesic molecules.
    Type: Application
    Filed: December 27, 2012
    Publication date: July 11, 2013
    Applicant: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENE ALT
    Inventor: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENE ALT
  • Patent number: 8470149
    Abstract: A method for dispensing liquid for use in biological analysis may comprise positioning liquid to be dispensed via electrowetting. The positioning may comprise aligning the liquid with a plurality of predetermined locations. The method may further comprise dispensing the aligned liquid from the plurality of predetermined locations through a plurality of openings respectively aligned with the predetermined locations. The dispensing may be via electrowetting.
    Type: Grant
    Filed: December 5, 2011
    Date of Patent: June 25, 2013
    Assignee: Applied Biosystems, LLC
    Inventors: Charles S. Vann, Debjyoti Banerjee, Timothy G. Geiser, James C. Nurse, Nigel P. Beard
  • Publication number: 20130140181
    Abstract: The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and comprise a main flow channel; a plurality of branch flow channels; a plurality of control channels; and a plurality of valves. Preferably, each of the valves comprises one of the control channels and an elastomeric segment that is deflectable into or retractable from the main or branch flow channel upon which the valve operates in response to an actuation force applied to the control channel.
    Type: Application
    Filed: September 4, 2012
    Publication date: June 6, 2013
    Applicant: California Institute of Technology
    Inventors: Stephen R. Quake, Marc A. Unger, Hou-Pu Chou, Todd A. Thorsen, Axel Scherer
  • Patent number: 8377277
    Abstract: Electrophoresis systems and methods comprise an electrophoresis device, wherein the electrophoresis comprises a loading channel, a separation channel, and an injection channel. The loading channel is in fluid communication with a first and second sample port. The separation channel is connected to the loading channel to form a first intersection, and an injection channel connected to the separation channel to form a second intersection and in fluid communication with a first reservoir, and wherein the separation channel is in fluid communication with a second reservoir. The electrophoresis system further comprises two electrodes coupled to the first sample port and the first reservoir, and the first sample port and the second reservoir, respectively, that are adapted to move the sample into the loading channel towards the first reservoir and form a sample plug in the separation channel, and to further move the sample plug into the separation channel towards the second reservoir.
    Type: Grant
    Filed: October 22, 2008
    Date of Patent: February 19, 2013
    Assignee: General Electric Company
    Inventors: Jun Xie, Wei-Cheng Tian, Shashi Thutupalli, Li Zhu, Anthony John Murray, Erin Jean Finehout