With Adjustment Or Alteration Of Electro-osmotic Bulk Flow Patents (Class 204/454)
  • Patent number: 10352898
    Abstract: A capillary ionic transistor and method of using is disclosed. The method including providing a capillary pipette (100) having an inner surface defining a channel, and a conductive layer disposed (102) about electrode the channel; filling at least a portion of the channel with an ionic solution (110) such that an electrical double layer forms on the inner surface of the pipette; inducing an electric potential within the ionic solution sufficient to generate a longitudinal flow of ions within the channel; and inducing an electric potential in the conductive layer sufficient to alter the zeta potential of the electrical double layer and adjust the flow of ions within the ionic solution.
    Type: Grant
    Filed: February 26, 2015
    Date of Patent: July 16, 2019
    Assignee: UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDAING, INC.
    Inventors: Yauheni Rudzevich, Yuqing Lin, Lee Chow
  • Patent number: 10312070
    Abstract: Methods, systems and devices that generate differential axial transport in a fluidic device having at least one fluidic sample separation flow channel and at least one ESI emitter in communication with the at least one sample separation flow channel. In response to the generated differential axial transport, the at least one target analyte contained in a sample reservoir in communication with the sample separation channel is selectively transported to the at least one ESI emitter while inhibiting transport of contaminant materials contained in the sample reservoir toward the at least one ESI emitter thereby preferentially directing analyte molecules out of the at least one ESI emitter. The methods, systems and devices are particularly suitable for use with a mass spectrometer.
    Type: Grant
    Filed: October 25, 2018
    Date of Patent: June 4, 2019
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: John Scott Mellors, John Michael Ramsey, Nicholas George Batz
  • Patent number: 10259744
    Abstract: A process for producing an optical glass with an anti-fog coating is disclosed. The process includes the steps of: a) providing an optical glass, b) preparing a layer having Si—H groups (silane groups) on the optical glass, and c) reacting the silane groups with a compound having hydrophilic groups and at least one group reactive to the silane group.
    Type: Grant
    Filed: July 3, 2017
    Date of Patent: April 16, 2019
    Assignee: Carl Zeiss Vision International GmbH
    Inventor: Thomas Glöge
  • Patent number: 10134576
    Abstract: Methods, systems and devices that generate differential axial transport in a fluidic device having at least one fluidic sample separation flow channel and at least one ESI emitter in communication with the at least one sample separation flow channel. In response to the generated differential axial transport, the at least one target analyte contained in a sample reservoir in communication with the sample separation channel is selectively transported to the at least one ESI emitter while inhibiting transport of contaminant materials contained in the sample reservoir toward the at least one ESI emitter thereby preferentially directing analyte molecules out of the at least one ESI emitter. The methods, systems and devices are particularly suitable for use with a mass spectrometer.
    Type: Grant
    Filed: November 13, 2017
    Date of Patent: November 20, 2018
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: John Scott Mellors, John Michael Ramsey, Nicholas George Batz
  • Patent number: 9818594
    Abstract: Methods, systems and devices that generate differential axial transport in a fluidic device having at least one fluidic sample separation flow channel and at least one ESI emitter in communication with the at least one sample separation flow channel. In response to the generated differential axial transport, the at least one target analyte contained in a sample reservoir in communication with the sample separation channel is selectively transported to the at least one ESI emitter while inhibiting transport of contaminant materials contained in the sample reservoir toward the at least one ESI emitter thereby preferentially directing analyte molecules out of the at least one ESI emitter. The methods, systems and devices are particularly suitable for use with a mass spectrometer.
    Type: Grant
    Filed: October 4, 2016
    Date of Patent: November 14, 2017
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: John Scott Mellors, John Michael Ramsey, Nicholas George Batz
  • Patent number: 9347440
    Abstract: A flow cell for use in a microfluidic detection system. The flow cell includes a flow cell body having a channel that extends along the flow cell body. The flow cell body has a substrate material that extends along the channel and that is transparent to at least one of excitation light or light emissions. The channel has a functionalized channel surface that includes reactive groups configured to attach to molecules for biochemical analysis. The flow cell also includes fluidic inlet and fluidic outlet ports provided on the flow cell body and an electroosmotic (EO) pump held within the flow cell body in fluid communication with the channel. The EO pump is operable to induce flow of a solution from the inlet port to the outlet port and along the functionalized channel surface.
    Type: Grant
    Filed: April 9, 2012
    Date of Patent: May 24, 2016
    Assignee: Illumina, Inc.
    Inventors: Michal Lebl, Dale Buermann, Mark T. Reed, David L. Heiner, Alexander Triener
  • Patent number: 9283563
    Abstract: In one aspect, the present invention provides a systems and methods for the real-time amplification and analysis of a sample of DNA.
    Type: Grant
    Filed: October 28, 2008
    Date of Patent: March 15, 2016
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Gregory H. Owen, Gregory A. Dale, Kenton C. Hasson, Shulin Zeng, Dwayne W. Warfield, Sarah Warfield
  • Patent number: 9266106
    Abstract: A convenient technique allows detecting fluorescence emitted in the channel with a uniformly high detection sensitivity and a good reproducibility. On the front face side of a plate-like body 11, a groove-shaped introduction channel 12 and separation channel 14 are formed. In this micro-channel chip 10, on the inner faces of a groove constituting the separation channel 14 formed in a surface of the plate-like body 11, there is formed a fine particle layer 20 provided by sintering fine particles having an average particle diameter of 10 to 500 nm mainly composed of zinc oxide (ZnO) or titanium oxide (TiO2).
    Type: Grant
    Filed: February 25, 2013
    Date of Patent: February 23, 2016
    Assignee: NATIONAL UNIVERSITY CORPORATION SHIMANE UNIVERSITY
    Inventors: Yasuhisa Fujita, Haruo Takeshita, Junko Fujihara, Yutaka Fukui, Mari Tabuchi
  • Patent number: 9121821
    Abstract: A process for analyzing a sample by a capillary electrophoresis method is provided that allows for high analytic precision and reduction in apparatus size, and can be readily carried out by electrophoresing a complex of a sample and an anionic group-containing compound in the capillary channel, wherein the capillary channel includes an A layer that is coated on an inner wall thereof and a B layer that is coated on the A layer, where the A and B layers are as described.
    Type: Grant
    Filed: August 29, 2007
    Date of Patent: September 1, 2015
    Assignees: National Institute of Advanced Industrial Science and Technology, ARKRAY, Inc.
    Inventors: Yoshihide Tanaka, Shinichi Wakida, Yusuke Nakayama, Satoshi Yonehara
  • Patent number: 9101928
    Abstract: An array of transportable particle sets is used in a microfluidic device for performing chemical reactions in the microfluidic device. The microfluidic device comprises a main channel and intersecting side channels, the main channel and side channels forming a plurality of intersections. The array of particle sets is disposed in the main channel, and the side channels are coupled to reagents. As the particle sets are transported through the intersections of the main channel and the side channels, reagents are flowed through the side channels into contact with each array member (or selected array members), thereby providing a plurality of chemical reactions in the microfluidic system.
    Type: Grant
    Filed: January 27, 2011
    Date of Patent: August 11, 2015
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Tammy Burd Mehta, Anne R. Kopf-Sill, J. Wallace Parce, Andrea W. Chow, Luc J. Bousse, Michael R. Knapp, Theo T. Nikiforov, Steve Gallagher
  • Patent number: 8961763
    Abstract: Provided is a device comprising an upper chamber, a middle chamber and a lower chamber, wherein the upper chamber is in communication with the middle chamber through a first pore, and the middle chamber is in communication with the lower chamber through a second pore, wherein the first pore and second pore are about 1 nm to about 100 nm in diameter, and are about 10 nm to about 1000 nm apart from each other, and wherein each of the chambers comprises an electrode for connecting to a power supply. Methods of using the device are also provided, in particular for sequencing a polynucleotide.
    Type: Grant
    Filed: July 18, 2012
    Date of Patent: February 24, 2015
    Assignee: The Regents of the University of California
    Inventors: William Dunbar, Jungsuk Kim
  • Patent number: 8834696
    Abstract: The present invention provides a device and methods of use thereof for desalting a solution. The methods, inter-alia, make use of a device comprising microchannels, which are linked to conduits, whereby induction of an electric field in the conduit results in the formation of a space charge layer within the microchannel. The space charge layer provides an energy barrier for salt ions and generates an ion depletion zone proximal to the linkage region between the microchannel and the conduit. The method thus enables the removal of salt ions from the region proximal to the conduit and their accumulation in a region distant from the conduit, within the microchannel.
    Type: Grant
    Filed: January 21, 2011
    Date of Patent: September 16, 2014
    Assignee: Massachusetts Institute of Technology
    Inventors: Sung Jae Kim, Jongyoon Han
  • Patent number: 8784631
    Abstract: Disclosed herein is a composition of a separation medium and method of its use for electrophoresis in bare channels, either capillaries or chips, with suppressed electroosmotic flow, wherein various forms of boric acid are adsorbed on the wall of said separation channel, efficiently suppressing zeta potential of the wall of said bare channel. A composition of a sieving separation medium for electrophoresis of DNA is disclosed. A composition of a sieving separation medium for electrophoretic size separation of proteins by SDS CSE is also disclosed. A composition of a separation medium for capillary electrophoresis without sieving is also disclosed.
    Type: Grant
    Filed: January 1, 2012
    Date of Patent: July 22, 2014
    Inventor: Vladislav Dolnik
  • Patent number: 8784626
    Abstract: A detection optics configuration for bio-analysis, in which the direction of incident radiation, the axis of the separation channel, and the direction of collection of the output radiation are coplanar at the detection zone. The detection configuration incorporates ball-end optical fibers to direct incident radiation at and collection of output radiation from the detection zone. The detection optics configuration may be implemented in an improved bio-separation instrument, in particular a capillary electrophoresis instrument.
    Type: Grant
    Filed: January 28, 2011
    Date of Patent: July 22, 2014
    Assignee: Bioptic, Inc.
    Inventors: Varouj D. Amirkhanian, Shou-Kuan Tsai
  • Patent number: 8778155
    Abstract: A cartridge-based bio-separation system configured to utilize a pen shaped bio-separation cartridge that is easy to assemble and use with no moving parts and that has an integrated reagent (separation buffer) reservoir. The cartridge includes a body, defining an opening as a detection window for receiving external detection optics, at least one capillary column supported in the body, having a first end extending beyond a first end of the body, wherein the detection window exposes a section along the capillary column, to which the external optics are aligned through the detection window, and a reservoir attached to a second end of the body in fluid flow communication with a second end of the capillary column. The reservoir is structured to be coupled to an air pressure pump that pressurizes the gel reservoir to purge and fill the capillaries with buffer as the separation support medium.
    Type: Grant
    Filed: August 18, 2011
    Date of Patent: July 15, 2014
    Assignee: Bioptic, Inc.
    Inventors: Shou-Kuan Tsai, Varouj D. Amirkhanian
  • Patent number: 8715475
    Abstract: A microfluidic device for separating, fractionating, or preconcentrating analytes contained in an electrolyte having at least two reservoirs separated by at least one microchannel and/or nanochannel. At least part of the wall of the microchannel is made of and/or coated interiorly with a conducting and polarizable material or group of materials constituting a polarizable interface or a network of polarizable interfaces. In that at least one electrode or at least one electrode network is connected at at least one point of the polarizable material or group of materials, the surface electrical conductance of said material being equal to at least 100 nS.
    Type: Grant
    Filed: November 3, 2009
    Date of Patent: May 6, 2014
    Assignee: Etat Francais Represente Par le Delegue General pour l'Armement
    Inventor: Adrien Plecis
  • Publication number: 20140034499
    Abstract: A microfluidic control apparatus operating method is disclosed. The microfluidic control apparatus operating method is applied in a microfluidic control apparatus, and the microfluidic control apparatus includes a photoconductive material layer and a flow passage. The microfluidic control apparatus operating method includes steps of (a) when a light with a specific optical pattern is emitted toward the photoconductive material layer, at least three virtual electrodes being formed on the photoconductive material layer according to the specific optical pattern; (b) when the specific optical pattern changes, the at least three virtual electrodes also changing to generate an electro-osmotic force to control a moving state of a microfluid in the flow passage.
    Type: Application
    Filed: October 7, 2013
    Publication date: February 6, 2014
    Applicant: CRYSTALVUE MEDICAL CORPORATION
    Inventors: Cheng-Hsien LIU, William WANG, Long HSU, Yuh-Shyong YANG, Hwan-You CHANG, Shih-Mo YANG, Chung-Cheng CHOU
  • Publication number: 20130277218
    Abstract: Devices and methods are provided for performing droplet-based solid phase processing steps on a digital microfluidic device. A solid phase material, which may be a porous solid phase material such as a porous polymer monolith is formed or located on a digital microfluidic element. The solid phase may be formed by an in-situ method in which the digital microfluidic array is actuated to transport a droplet of solid phase pre-cursor solution to a selected element on the array, and subsequently processed to form a solid phase on the array element. The integration of a solid phase material with a digital microfluidic array enables a wide range of applications including solid phase extraction and sample concentration.
    Type: Application
    Filed: October 3, 2011
    Publication date: October 24, 2013
    Applicant: THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO
    Inventors: Jared M. Mudrik, Hao Yang, Aaron R. Wheeler
  • Publication number: 20130220811
    Abstract: A system that incorporates the subject disclosure may include, for example, a method for generating an electric or pressure difference force that induces a plurality of particles to flow through a through-hole. Independently adjustable heat source in a vicinity of the through-hole induces a thermodynamic force for modifying the flow of the plurality of particles through the through-hole. Additional embodiments are disclosed.
    Type: Application
    Filed: February 22, 2013
    Publication date: August 29, 2013
    Applicant: THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOI S
    Inventor: The Board of Trustees of The University of Illinois
  • Patent number: 8518227
    Abstract: A nanoparticle translocation device includes a first reservoir having a first reservoir electrode, a second reservoir having a second reservoir electrode, and at least one nanopore providing fluid communication between the first and second reservoirs. The device also includes one or more inner electrode portions on an inner wall of the nanopore and one or more outer electrode portions disposed on an outer wall of the nanopore. The device further includes at least one DC voltage supply for selectively applying a DC voltage to each of the first reservoir electrode, the second reservoir electrode, and the outer electrode layer, where the inner electrode portions, the outer electrode portions, and the nanopore are in a substantially coaxial arrangement.
    Type: Grant
    Filed: September 15, 2011
    Date of Patent: August 27, 2013
    Assignee: Old Dominion University Research Foundation
    Inventors: Shizhi Qian, Ali Beskok
  • Patent number: 8481125
    Abstract: Molecular adsorption to the microfluidic device surfaces can be passively and actively mitigated by mixing certain hydrophilic polymers (organic polymers with repeating hydrophilic groups—the preferred polymers being amphipathic surfactants—with the sample liquid during or prior to relevant microfluidic operations. Nonionic surfactants such as polyoxyethylene sorbitan monooleate and polyoxyethylene octyl phenyl ether are especially effective. High molecular weight polyethylene polymers are also effective. The hydrophilic polymers appear to prevent binding of the fouling molecules to the microfluidic by occupying the surface sites in place of the fouling molecules or by interacting with the fouling molecules to prevent binding of the fouling molecules the surface. When surface adsorption is thus mitigated, microfluidic devices can readily handle samples containing biomolecules to enable active sample concentration, filtering, washing, transport, mixing and other sample handling operations.
    Type: Grant
    Filed: May 18, 2006
    Date of Patent: July 9, 2013
    Assignee: Advanced Liquid Logic Inc.
    Inventors: Uichong B. Yi, Peter-Patrick De Guzman, Wayne Po-Wen Liu
  • Publication number: 20130146459
    Abstract: This invention provides devices and apparatuses comprising the same and methods of use thereof for efficient pumping and/or mixing of relatively small volumes of fluid, wherein the fluid contains a sample within an inner fluid phase dispersed in an outer phase. Such devices utilize nonlinear electrokinetics as a primary mechanism for driving fluid flow and/or mixing the fluid. Methods of cellular analysis, drug delivery and others, utilizing the devices are described.
    Type: Application
    Filed: June 15, 2010
    Publication date: June 13, 2013
    Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Martin Bazant, Manu Prakash
  • Patent number: 8444836
    Abstract: A device for forming at least one circulating flow, or vortex, at the surface of a drop of liquid, including at least two first electrodes forming a plane and having edges facing each other, such that the contact line of a drop, deposited on the device and fixed relatively to the device, has a tangent forming, when projected onto the plane of the electrodes, an angle between 0° and 90° with the edges facing each other of the electrodes.
    Type: Grant
    Filed: December 3, 2007
    Date of Patent: May 21, 2013
    Assignees: Commissariat a l'Energie Atomique, Centre National de la Recherche Scientifique
    Inventors: Yves Fouillet, Laurent Davoust
  • Patent number: 8303789
    Abstract: Various embodiments provide an exemplary lab-on-a-chip (LOC) system that serves as an analytical tool and/or as a separation medium for an electrolyte solution including various charged molecular species. The LOC system can include an integrated nanofluidic FET device in combination with suitable analysis systems. By applying and controlling a longitudinal electric field and a transverse electric potential, the flow and the pH of the electrolyte solution in the nanofluidic channels can be controlled.
    Type: Grant
    Filed: March 11, 2010
    Date of Patent: November 6, 2012
    Assignee: STC.UNM
    Inventors: Cornelius F. Ivory, Sang M. Han, Youn-Jin Oh
  • Patent number: 8298393
    Abstract: A device for electrophoretic analysis of multicomponent solutions comprises a capillary and vials for electrolyte and sample solutions. The device comprises streaming potential measurement means implemented to measure a potential difference between capillary ends and to form an electric connections with said capillary ends during capillary rinsing in such a way that said streaming potential measurement means and electrolyte inside capillary and vials form a closed measurement electric circuit. A means for generation of electrolyte flow is implemented so as to build up and maintain a preset differential pressure between capillary ends and wherein that streaming potential measurement means comprises means to disconnect said measurement electric circuit during electrophoretic analysis.
    Type: Grant
    Filed: February 5, 2007
    Date of Patent: October 30, 2012
    Assignee: Lumex Instruments Limited
    Inventors: Mikhail Ivanovich Zinchenko, Yaroslav Sergeevich Kamentsev, Mikhail Yaroslavovich Kamentsev, Slyadnev Nikolaevich Maksim, Alexander Anatolevich Stroganov
  • Patent number: 8182746
    Abstract: An electrophoresis apparatus is generally disclosed for sequentially analyzing a single sample or multiple samples having one or more analytes in high or low concentrations. The apparatus comprises a relatively large-bore transport capillary which intersects with a plurality of small-bore separation capillaries and includes a valve system. Analyte concentrators, having antibody-specific (or related affinity) chemistries, are stationed at the respective intersections of the transport capillary and separation capillaries to bind one or more analytes of interest. The apparatus allows the performance of two or more dimensions for the optimal separation of analytes. The apparatus may also include a plurality of valves surrounding each of the analyte concentrators to localize each of the concentrators to improve the binding of one or more analytes of interest.
    Type: Grant
    Filed: October 31, 2007
    Date of Patent: May 22, 2012
    Assignee: Princeton Biochemicals, Inc.
    Inventor: Norberto A. Guzman
  • Publication number: 20120043209
    Abstract: A microfluidic control apparatus and operating method thereof. The microfluidic control apparatus includes a photoconductive material layer and a flow passage. When a light with a specific optical pattern is emitted toward the photoconductive material layer, at least three virtual electrodes are formed on the photoconductive material layer according to the specific optical pattern. The at least three virtual electrodes include a first virtual electrode, a second virtual electrode and a third virtual electrode disposed beside the first virtual electrode. There is a specific proportion among a distance between first virtual electrode and third virtual electrode, a width of first virtual electrode, a distance between first virtual electrode and second virtual electrode, and a width of second virtual electrode. When the specific optical pattern changes, the at least three virtual electrodes also change to generate an electro-osmotic force to control the moving state of a microfluid in a flow passage.
    Type: Application
    Filed: August 18, 2011
    Publication date: February 23, 2012
    Inventors: Cheng-Hsien Liu, William Wang, Long Hsu, Yuh-Shyong Yang, Hwan-You Chang, Shih-Mo Yang, Chung-Cheng Chou
  • Patent number: 8101057
    Abstract: Methods and devices are described for concentration and cleanup of samples containing bio-molecule analytes (e.g., polynucleotides, such as DNA, RNA, PNA). Various embodiments provide for pH-mediated sample concentration and cleanup of nucleic acid samples with channel devices (e.g., cross-T format, microchannel devices).
    Type: Grant
    Filed: January 13, 2010
    Date of Patent: January 24, 2012
    Assignee: Applied Biosystems, LLC
    Inventor: Karl Voss
  • Patent number: 8097140
    Abstract: A method for collecting an analyte species from a sample is provided, the method of collection potentially being supplemented to give a method of preparing a sample for analysis and/or a method of analysis. The method including providing part of a sample in a substrate, causing the sample to migrate to an interface between the substrate and a second substrate due to the action of an electrical potential difference, the electrophoretic velocity of the analyte species of the second substrate being balanced by or exceeded by the bulk flow velocity of the second substrate and the bulk flow velocity of the second substrate being in an opposing direction to the electrophoretic velocity of the analyte species in the second substrate. In this way substantial concentration of the analyte species at the interface is provided. Subsequently the species can be conveyed away from the interface for further preparation and/or analysis.
    Type: Grant
    Filed: June 8, 2007
    Date of Patent: January 17, 2012
    Assignee: Forensic Science Service Ltd.
    Inventors: Silvia Valussi, Andreas Manz
  • Patent number: 8057191
    Abstract: The invention is directed to the elimination of changes of the chemical composition of a pumped liquid caused by introduction of strange components or by modification of original components. Another object of the invention is to provide the possibility of use of electrodes of the first order in order to increase productivity and decrease size and cost of the micropump. For this purpose, the electrokinetic micropump comprises a multichannel structure 810 made of non-conducting material, for example, a piece of a polycapillary column. The inlet and outlet end of this structure are adjacent to electrode sections 803, 804 having openings 821, 822 for inlet and outlet of the pumped liquid. These sections are divided by ion-exchange membranes 811, 812 into chambers 813, 814 for flow of the pumped liquid, communicating with the ends 841, 842 of the multichannel structure, and chambers 815, 816 filled with an auxiliary medium for transfer of electric charges. In the latter electrodes 817, 818 are located.
    Type: Grant
    Filed: June 29, 2006
    Date of Patent: November 15, 2011
    Assignee: Obshschestvo S Orgranichennoj Otvetstvennostyu “Institu Rentgenovskoj Optiki”
    Inventors: Ruslan Khazhsetovich Khamizov, Muradin Abubekirovich Kumakhov, Natalia Sergeevna Bastrykina, Svetlana Vassilievna Nikitina, Alexandr Alexandrovich Voronov, Ruslan Khazhsetovich Khamizov, legal representative
  • Patent number: 8030092
    Abstract: An electrophoresis apparatus is generally disclosed for sequentially analyzing a single sample or multiple samples having one or more analytes in high or low concentrations. The apparatus comprises a relatively large-bore transport capillary which intersects with a plurality of small-bore separation capillaries and includes a valve system. Analyte concentrators, having antibody-specific (or related affinity) chemistries, are stationed at the respective intersections of the transport capillary and separation capillaries to bind one or more analytes of interest. The apparatus allows the performance of two or more dimensions for the optimal separation of analytes. The apparatus may also include a plurality of valves surrounding each of the analyte concentrators to localize each of the concentrators to improve the binding of one or more analytes of interest.
    Type: Grant
    Filed: November 17, 2006
    Date of Patent: October 4, 2011
    Assignee: Princeton Biochemicals, Inc.
    Inventor: Norberto A. Guzman
  • Patent number: 8019577
    Abstract: A method for modeling turbidite channels which may contain oil that it is desired to extract is provided in which the final shape of the complex resulting from migration of elementary channels over the course of deposition and which is observable from seismic data is combined with the implementation of simple geometric rules, comprising simulation of one or several intermediate channels passing through points on trajectories running from a final channel to an initial channel. The trajectories involved are different from the channel axes or lines; they reflect channel migration over time.
    Type: Grant
    Filed: November 19, 2007
    Date of Patent: September 13, 2011
    Assignee: Total S.A.
    Inventor: Richard Labourdette
  • Publication number: 20110198225
    Abstract: The present invention provides a device and methods of use thereof for desalting a solution. The methods, inter-alia, make use of a device comprising microchannels, which are linked to conduits, whereby induction of an electric field in the conduit results in the formation of a space charge layer within the microchannel. The space charge layer provides an energy barrier for salt ions and generates an ion depletion zone proximal to the linkage region between the microchannel and the conduit. The method thus enables the removal of salt ions from the region proximal to the conduit and their accumulation in a region distant from the conduit, within the microchannel.
    Type: Application
    Filed: January 21, 2011
    Publication date: August 18, 2011
    Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Sung Jae Kim, Jongyoon Han
  • Publication number: 20110192725
    Abstract: The invention provides uncharged water-soluble silica-adsorbing polymers for suppressing electroendoosmotic flow and to reduce analyte-wall interactions in capillary electrophoresis. In one aspect of the invention, one or more of such polymers are employed as components of a separation medium for the separation of biomolecules, such as polynucleotides, polysaccharides, proteins, and the like, by capillary electrophoresis. Generally, such polymers are characterized by (i) water solubility over the temperature range between about 20° C. to about 50° C., (ii) concentration in a separation medium in the range between about 0.001% to about 10% (weight/volume), (iii) molecular weight in the range of about 5×103 to about 1×106 daltons, and (iv) absence of charged groups in an aqueous medium having pH in the range of about 6 to about 9.
    Type: Application
    Filed: December 23, 2010
    Publication date: August 11, 2011
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Ramakrishna S. Madabhushi, Steven M. Menchen, J. William Efcavitch, Paul D. Grossman
  • Patent number: 7857955
    Abstract: The invention is directed to a capillary tube for electrophoresis that has a positively charged coating on the capillary inner surface that prevents positively charged analytes from adsorbing to the inner capillary surface. The capillary tube has an inner surface that is coated with a first polymer layer having a plurality of polymer groups comprising polyethylene imine, designated herein as (CH2CH2NH)x. The inner surface of the capillary typically has a second polymer layer covalently bonded to the first polymer layer. The invention includes a capillary tube where two or more than two polymer groups are covalently bonded to each other by a cross-linker. Also provided are an electrophoresis system the uses the coated capillary tubes, a method of performing electrophoresis that utilizes the coated capillary tubes, and a process for preparing the coated capillary tubes.
    Type: Grant
    Filed: June 10, 2005
    Date of Patent: December 28, 2010
    Assignee: Beckman Coulter, Inc.
    Inventors: Chitra K. Ratnayake, Isabel C. Flores
  • Publication number: 20100320088
    Abstract: A device for forming at least one circulating flow, or vortex, at the surface of a drop of liquid, including at least two first electrodes forming a plane and having edges facing each other, such that the contact line of a drop, deposited on the device and fixed relatively to the device, has a tangent forming, when projected onto the plane of the electrodes, an angle between 0° and 90° with the edges facing each other of the electrodes.
    Type: Application
    Filed: December 3, 2007
    Publication date: December 23, 2010
    Applicants: Commissariat A L'Energie, Centre National De La Recherche Scient
    Inventors: Yves Fouillet, Laurent Davoust
  • Patent number: 7837848
    Abstract: A method for separating the constituents of a mixture M by electrophoresis in a single capillary includes (A) separating compounds of the mixture M in a single capillary according to the capillary electrophoresis technique; (B) isolating a fraction F of the compounds thus separated by evacuating part of the compounds having the highest migration speeds from the capillary and/or evacuating part of the compounds having the lowest migration speeds from the capillary; (C) introducing a separating medium MS having a higher migration speed than the compounds of the isolated fraction F into the capillary containing the isolated fraction F, and (D) the compounds contained in fraction F are separated in the new electrophoretic conditions thus obtained.
    Type: Grant
    Filed: August 23, 2004
    Date of Patent: November 23, 2010
    Assignees: Centre National de la Recherche Scientifique (C.N.R.S.), Universite de Montpellier II
    Inventors: Jean-Philippe Biron, Hervé Cottet
  • Publication number: 20100155243
    Abstract: A separation module operates to fractionate or separate an analyte into fractions according to pI, i.e., pI bands, utilizing capillary isoelectric focusing (“CIEF”) within a first microchannel. The fractions are stacked to form plugs, the number of which is determined by a number of parallel second microchannels integrally connected to the first microchannel, into which the fractions are directed according to the buffer characteristics found in each of the individual microchannels. Within the microchannels the plugs are separated into proteins according to a different chemical property, i.e., “m/z,” utilizing capillary electrophoresis (“CE”).
    Type: Application
    Filed: December 22, 2009
    Publication date: June 24, 2010
    Inventors: Thomas Wayne Schneider, James N. Baraniuk
  • Patent number: 7740747
    Abstract: A microchip for capillary electrophoresis is provided. The microchip comprises an injection channel and a separation channel configured to receive a sample through a sample well disposed on a first end of the separation channel; wherein the injection channel and the separation channel intersect to form a ‘T’ junction. The microchip further comprises a first valve disposed adjacent to the ‘T’ junction and on the separation channel and a second valve disposed at the ‘T’ junction. The second valve is a two-way valve. A sample plug is injected into an area between the ‘T’ junction and a second end of the separation channel.
    Type: Grant
    Filed: December 28, 2007
    Date of Patent: June 22, 2010
    Assignee: General Electric Company
    Inventors: Wei-Cheng Tian, Erin Jean Finehout, Li Zhu, Jun Xie, Shashi Thutupalli
  • Patent number: 7722752
    Abstract: A microfluidic device for carrying a liquid, the device comprising a microfluidic channel having an interior wall and a polyelectrolyte film on the interior wall whereby liquid carried by the channel contacts the polyelectrolyte film, the polyelectrolyte film having a thickness of about 1 to about 1000 nanometers and comprising an interpenetrating network of a predominantly positively charged polymer and a predominantly negatively charged polymer, the predominantly positively charged polymer, the predominantly negatively charged polymer or both containing (i) a pH insensitive positively or negatively charged repeat unit having a pKa greater than 9 or less than 3, and (ii) a pH sensitive repeat unit, the pH sensitive repeat unit having a pKa of 3 to 9, whereby the pH of liquid in the microfluidic channel may be used to control the velocity or direction of electroosmotic flow of the liquid within said microfluidic channel.
    Type: Grant
    Filed: March 2, 2005
    Date of Patent: May 25, 2010
    Assignee: Florida State University Research Foundation
    Inventors: Joseph B. Schlenoff, Zhijie Sui
  • Publication number: 20100108514
    Abstract: To suppress temperature variations of sample fluids within flow channels for electrophoresis, in a method for controlling temperatures within micro flow channels. When controlling the temperatures of sample fluids within micro flow channels of electrophoresis chips, in which flow channels through which electrophoresis occurs by application of electrical potential differences can be switched, temperature variations of the sample fluids within the micro flow channels, caused by differences in heat generated by the sample fluids prior to and following the switching of the flow channels, are predicted. Control properties for temperature control in order to cancel the temperature variations are changed during the switching of the flow channels.
    Type: Application
    Filed: March 28, 2008
    Publication date: May 6, 2010
    Applicant: FUJIFILM COPORATION
    Inventors: Yoshihiro Seto, Tomohisa Kawabata, Chungsoo Charles Park
  • Publication number: 20090255601
    Abstract: A fluidic device, the fluidic device comprising a planar fluidic conduit for conducting a fluid, wherein the fluidic conduit has a plurality of fluidic disturbance features located along at least a section of the fluidic conduit for disturbing a laminar flow of the fluid along the section.
    Type: Application
    Filed: April 14, 2008
    Publication date: October 15, 2009
    Applicant: AGILENT TECHNOLOGIES, INC.
    Inventors: Martin BAEUERLE, Konstantin CHOIKHET
  • Patent number: 7597790
    Abstract: A flow controller which uses a combination of hydrostatic pressure and electroosmotic flow to control the flow of a fluid. A driving fluid (1204) whose flow rate is dependent on both hydrostatic pressures and electroosmotic flow can be used (a) directly as a working fluid in an operable device, for example a chromatograph, or (b) to displace a working fluid (1203) from a storage container (625) into an operable device (1301), or both (a) and (b). The driving fluid (1204) can be composed of one or more fluids. Part or all the driving fluid (1204) is passed through an electroosmotic device (100) so as to increase or decrease the flow rate induced by hydrostatic pressure.
    Type: Grant
    Filed: June 13, 2002
    Date of Patent: October 6, 2009
    Assignee: Eksigent Technologies, LLC
    Inventors: David W. Neyer, Phillip H. Paul, Don Wesley Arnold, Christopher G. Bailey
  • Patent number: 7439075
    Abstract: A method of discharging a fluid flow with suspended microparticles from a fluidic microsystem (10) is described, whereby the fluid flow converges with at least one output flow to form a discharge flow at the end of a discharge channel (14) of the microsystem, and the discharge flow is delivered through a conduction element (19). A microsystem with a flow output device for implementation of this method is also described.
    Type: Grant
    Filed: February 8, 2001
    Date of Patent: October 21, 2008
    Assignee: Perkinelmer Cellular Technologies Germany GmbH
    Inventors: Gunter Fuhr, Stephen Graham Shirley, Torsten Muller, Thomas Schnelle, Rolf Hagedorn
  • Patent number: 7431888
    Abstract: A microfluidic device preferably made of a thermoplastic polymer that includes a channel or a multiplicity of channels whose surfaces are modified by photografting. The device further includes a porous polymer monolith prepared via UV initiated polymerization within the channel, and functionalization of the pore surface of the monolith using photografting. Processes for making such surface modifications of thermoplastic polymers and porous polymer monoliths are set forth.
    Type: Grant
    Filed: September 19, 2003
    Date of Patent: October 7, 2008
    Assignee: The Regents of the University of California
    Inventors: Jean M. J. Frechet, Frantisek Svec, Thomas Rohr
  • Publication number: 20080128279
    Abstract: A microfabricated device employing a bridging membrane and methods for electrokinetic transport of a liquid phase biological or chemical material using the same are described. The bridging membrane is deployed in or adjacent to a microchannel and permits either ionic current flow or the transport of gas species, while inhibiting the bulk flow of material. The use of bridging membranes in accordance with this invention is applicable to a variety of processes, including electrokinetically induced pressure flow in a region of a microehannel that is not influenced by an electric field, sample concentration enhancement and injection, as well as improving the analysis of materials where it is desired to eliminate electrophoretic bias. Other applications of the bridging membranes according to this invention include the separation of species from a sample material, valving of fluids in a microchannel network, mixing of different materials in a microchannel, and the pumping of fluids.
    Type: Application
    Filed: October 19, 2007
    Publication date: June 5, 2008
    Inventors: Stephen C. Jacobson, J. Michael Ramsey, Christopher T. Culbertson, William B. Whitten, Robert S. Foote
  • Patent number: 7316543
    Abstract: An electroosmotic micropump having a plurality of thin, closely-spaced, approximately planar, transversel aligned partitions formed in or on a substrate, among which electroosmotic flow (EOF) is generated. Electrodes are located within enclosed inlet and outlet manifolds on either side of the partition array. Inlet and outlet ports enable fluid to be pumped into and through the micropump and through an external friction load or head. Insulating layer coatings on the formed substrate limit substrate leakage current during pumping operation.
    Type: Grant
    Filed: May 30, 2003
    Date of Patent: January 8, 2008
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Kenneth E. Goodson, Thomas W. Kenny, Juan G. Santiago, Daniel J. Laser, Chuan-Hua Chen
  • Patent number: 7285421
    Abstract: An apparatus and method for capillary zone electrophoresis includes a polyelectrolyte multilayer positioned in a capillary tube for analytical separations of macromolecules. The capillary comprises a passage defined by passage walls comprising fused silica. The polyelectrolyte multilayer is positioned within the passage adjacent the walls, and comprises an organic polyelectrolyte. The passage may further comprise nonporous silica particles coated with a multilayer including a plurality of polyelectrolyte layers. An apparatus includes a power supply having a positive electrode and a negative electrode for generating an electric field therebetween. The apparatus includes a capillary having a passage formed by passage walls and comprising therein a polyelectrolyte multilayer positioned substantially within the passage.
    Type: Grant
    Filed: September 2, 2004
    Date of Patent: October 23, 2007
    Assignee: Florida State University Research Foundation, Inc.
    Inventors: Joseph B. Schlenoff, Timothy W. Graul
  • Patent number: 7282127
    Abstract: A microcapillary device includes a microcapillary tube. An anode is positioned at a first end of the microcapillary tube. A cathode is positioned at a second end of said microcapillary tube. A plurality of electric field reducing components are spaced apart along a length of the microcapillary tube. The anode and the cathode generate an electric field along the length of the microcapillary tube, and the plurality of electric field reducing components selectively reduce the electric field at spatial intervals along the length of the microcapillary tube.
    Type: Grant
    Filed: April 7, 2005
    Date of Patent: October 16, 2007
    Assignee: East Carolina
    Inventor: Martin Bier
  • Patent number: 7252928
    Abstract: Methods for reducing surface adsorption of biological materials to the walls of microfluidic conduits in microscale devices are provided. In an example of the methods, one or more colloidal-size particles, such as colloidal silica particles, are flowed in a fluid within the microfluidic conduit in the presence of one or more adherent biological materials (such as one or more proteins, cells, carbohydrates, nucleic acids, lipids and the like) to adsorb to the materials and prevent them from binding to the capillary walls of the microfluidic conduit. Other adsorption inhibition agents such as detergents and nonaqueous solvents can be used alone or in combination with colloidal particles to reduce surface adsorption in microfluidic conduits.
    Type: Grant
    Filed: February 25, 2003
    Date of Patent: August 7, 2007
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Dean G. Hafeman, Aileen Zhou