Using Gel-filled Capillary Patents (Class 204/455)
  • Patent number: 10138356
    Abstract: The invention relates to a liquid composition comprising polymer chains and particles of an inorganic material in a liquid, an article comprising said liquid composition, a gel obtained from said liquid composition, an article comprising said gel, and the use of said liquid composition. The liquid composition comprises polymer chains and particles of an inorganic material in a liquid, wherein the polymer chains are linked to particles of the inorganic material by means of a functional group that is present in the polymer chains, wherein the polymer used has a lower critical solution temperature in the liquid used, and wherein the liquid composition exhibits thermo-induced gelation.
    Type: Grant
    Filed: April 24, 2009
    Date of Patent: November 27, 2018
    Assignee: NEDERLANDSE ORGANISATIE VOOR TOEGEPAST-NATUURWETENSCHAPPELIJK ONDERZOEK TNO
    Inventors: Eric Gerardus Maria Craenmehr, Aafke Tessa ten Cate
  • Patent number: 9951101
    Abstract: Novel compositions for removing impurities such as, protein aggregates, from a sample containing a protein of interest, e.g., an antibody. Such compositions can be used prior to the virus filtration step during protein purification, to remove aggregates and protect the virus filter from fouling, therefore improving virus filter capacity. A porous solid support including a co-polymer having at least two monomers, wherein at least one of the monomers comprises acrylamide and at least a second monomer comprises a hydrophobic binding group, where the solid support selectively binds protein aggregates, thereby to separate the monomeric protein of interest from the protein aggregates. The method can be performed under neutral to high pH and high conductivity conditions.
    Type: Grant
    Filed: November 6, 2014
    Date of Patent: April 24, 2018
    Assignee: EMD Millipore Corporation
    Inventors: Mikhail Kozlov, William Cataldo, Jeffrey Caron
  • Patent number: 9766206
    Abstract: An apparatus includes a body portion that defines a reservoir and a set of substantially flexible capillaries. The set of substantially flexible capillaries are fixedly coupled to the body portion and in fluid communication with the reservoir. A connector is configured to be coupled to the body portion to be in fluid communication with the reservoir and the set of substantially flexible capillaries. The connector is further configured to be coupled to a vacuum source. The apparatus is arranged such that at least a part of the body portion is electrically conductive. Methods for separating and detecting an analyte from a biological sample with the apparatus are also provided. For example, methods for separating and detecting one or more proteins from a cellular lysate or a purified protein are also provided.
    Type: Grant
    Filed: September 27, 2013
    Date of Patent: September 19, 2017
    Assignee: ProteinSimple
    Inventors: Tom W. Yang, David John Roach
  • Patent number: 9752081
    Abstract: A method that produces coupled radical products from biomass. The method involves obtaining a lipid or carboxylic acid material from the biomass. This material may be a carboxylic acid, an ester of a carboxylic acid, a triglyceride of a carboxylic acid, or a metal salt of a carboxylic acid, or any other fatty acid derivative. This lipid material or carboxylic acid material is converted into an alkali metal salt. The alkali metal salt is then used in an anolyte as part of an electrolytic cell. The electrolytic cell may include an alkali ion conducting membrane (such as a NaSICON membrane). When the cell is operated, the alkali metal salt of the carboxylic acid decarboxylates and forms radicals. Such radicals are then bonded to other radicals, thereby producing a coupled radical product such as a hydrocarbon. The produced hydrocarbon may be, for example, saturated, unsaturated, branched, or unbranched, depending upon the starting material.
    Type: Grant
    Filed: December 5, 2013
    Date of Patent: September 5, 2017
    Assignee: CERAMATEC, INC.
    Inventors: Mukund Karanjikar, Sai Bhavaraju, Ashok V Joshi, Pallavi Chitta, David Joel Hunt
  • Patent number: 9709538
    Abstract: Devices to detect a substance and methods of producing such a device are disclosed. An example device to detect a substance includes a housing defining an externally accessible chamber and a seal to enclose at least a portion of the chamber. The example device also includes a substrate includes nanoparticles positioned within the chamber. The nanoparticles to react to the substance when exposed thereto. The example device also includes a non-analytic solution within the chamber to protect the nanoparticles from premature exposure.
    Type: Grant
    Filed: September 30, 2011
    Date of Patent: July 18, 2017
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Wei Wu, Zhiyong Li, Ansoon Kim, Min Hu, Michael Josef Stuke
  • Patent number: 9500625
    Abstract: A microfluidic detection system for micrometer-sized entities, such as biological cells, includes a detector component incorporating a plate with a plurality of opening, the plate separating two chambers, one in communication with a fluid source containing target entities bound to magnetic beads. The openings are sized to always permit passage of the magnetic beads therethrough into a lower one of the chambers and are further sized to always prevent passage of the target entities from the upper one of the chambers. The detector component further includes a magnet positioned to pull unbound magnetic beads through the openings and to capture target entities bound to magnetic beads on the surface of the plate. In a further feature, the microfluidic detection system is configured to pass target molecules through the plate to be bound to a functionalized surface of the lower chamber.
    Type: Grant
    Filed: September 26, 2013
    Date of Patent: November 22, 2016
    Assignee: Purdue Research Foundation
    Inventors: Cagri A. Savran, Philip S. Low, Chun-Li Chang, Wanfeng Huang
  • Patent number: 9159537
    Abstract: Described herein is a method and system for on-line coupling of capillary isoelectric focusing (cIEF) to high-resolution mass spectrometry in which a sheath flow buffer comprising polar organic solvent and organic acid is used as both an immobilization solution for (cIEF) and an ionization solution for electrospray ionization (ESI).
    Type: Grant
    Filed: May 2, 2013
    Date of Patent: October 13, 2015
    Assignees: University of Notre Dame du Lac, Medimmune, LLC
    Inventors: James McGivney, Guijie Zhu, Norman Dovichi
  • Patent number: 9053914
    Abstract: A method of detecting one or more compounds, chemicals or contaminants in a substrate by mass spectrometry is disclosed. A non-living substrate is analyzed by contacting the substrate with a diathermy knife. An electric current is applied to the diathermy knife such that the diathermy knife vaporizes a portion of the substrate. The vapor is aspirated via a sampling tube pumped by a venturi pump into a vacuum chamber of a mass spectrometer. Analyte molecules are aspirated into the vacuum chamber whereupon they impact a surface of the vacuum chamber and are ionized to form analyte ions which are then mass analyzed.
    Type: Grant
    Filed: June 1, 2012
    Date of Patent: June 9, 2015
    Assignee: Micromass UK Limited
    Inventors: Steven Derek Pringle, Michael Raymond Morris, Stephen Smith, Michael McCullagh
  • Patent number: 9017941
    Abstract: A method for nucleic acid analysis including injecting a slight amount of nucleic acid sample for analysis, characterized in that most of a nucleic acid sample which is not used, excluding the slight amount of the nucleic acid sample which is used for analysis, can be obtained as a pure product which is not contaminated with a fluorescent material, and a microchip for analyzing nucleic acid which enables such method for nucleic acid analysis, are provided. The method for nucleic acid analysis may analyze at least two different nucleic acid samples in a continuous manner by sequentially injecting the at least two different nucleic acid samples.
    Type: Grant
    Filed: March 7, 2013
    Date of Patent: April 28, 2015
    Assignee: Postech Academy-Industry Foundation
    Inventors: Jong Hoon Hahn, Byoung Joo Kwak, Han-Ok Kim
  • Publication number: 20150001080
    Abstract: Embodiments of an electrophoresis device and methods for using the same in analyte separation applications are provided. Certain embodiments of the present disclosure include microfluidic devices having a single electrophoretic separation channel containing a separation medium. Systems according to embodiments of the present disclosure are configured to monitor analyte fronts moving through the electrophoretic separation channel in order to detect differentially migrating analytes, i.e., the systems are configured for moving boundary electrophoresis (MBE).
    Type: Application
    Filed: February 1, 2013
    Publication date: January 1, 2015
    Inventors: Amy E. Herr, Todd Duncombe
  • Publication number: 20140332383
    Abstract: Provided are devices that include a support, a free-standing polymeric separation medium associated with the support and configured to separate a sample along a directional axis, and a sample-loading element associated with the polymeric separation medium. Systems that include the devices, as well as methods of using the devices, are also provided. Embodiments of the present disclosure find use in a variety of different applications, including detecting whether an analyte is present in a fluid sample.
    Type: Application
    Filed: May 6, 2014
    Publication date: November 13, 2014
    Inventors: Amy E. Herr, Todd Duncombe
  • Publication number: 20140274660
    Abstract: A fabric phase sorptive extractor (FPSE) is a sampling device where a flexible fabric is coated with at least one sol-gel derived film that includes at least two of a metal oxide portion, a siloxy portion, and an organic portion, where the gel has at least some amorphous portions. The FPSE is flexible such that it can be used in an extended form or draped over a solid surface to contact a gaseous, liquid, or solid environment and can be manipulated for placement in a container where the absorbed analyte can be removed from the FPSE for instrumental analysis. The FPSE can have specific functionalities that bind to specific analytes or can provide a general sorbent medium for extraction of a wide range of analytes, such that the sampling device can be employed for sampling analytes with biological, environmental, food, pharmaceutical, bio-analytical, clinical, forensic, toxicological, national security, public health, and/or safety implications.
    Type: Application
    Filed: March 17, 2014
    Publication date: September 18, 2014
    Applicant: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES
    Inventors: Abuzar Kabir, Kenneth G. Furton
  • Patent number: 8784626
    Abstract: A detection optics configuration for bio-analysis, in which the direction of incident radiation, the axis of the separation channel, and the direction of collection of the output radiation are coplanar at the detection zone. The detection configuration incorporates ball-end optical fibers to direct incident radiation at and collection of output radiation from the detection zone. The detection optics configuration may be implemented in an improved bio-separation instrument, in particular a capillary electrophoresis instrument.
    Type: Grant
    Filed: January 28, 2011
    Date of Patent: July 22, 2014
    Assignee: Bioptic, Inc.
    Inventors: Varouj D. Amirkhanian, Shou-Kuan Tsai
  • Patent number: 8784627
    Abstract: The invention relates to graft copolymers, their preparation, and compositions, such as electrophoresis separation media, containing the same; also to ultra-high molecular weight poly(N,N-dimethylacrylamide) (“poly(DMA)”) polymers, their preparation, and compositions, such as electrophoresis separation media, containing the same; and more particularly to supports, such as capillaries, containing these polymers and methods for separating biomolecules, especially polynucleotides, using capillary electrophoresis. The graft copolymers can be prepared by, e.g., grafting polyacrylamide units onto a poly(DMA) backbone. Separation media comprising such graft copolymers or ultra-high molecular weight poly(DMA) polymers yield superior performance in the analysis and separation of biomolecules by capillary electrophoresis.
    Type: Grant
    Filed: September 23, 2009
    Date of Patent: July 22, 2014
    Assignee: Applied Biosystems, LLC
    Inventor: Aldrich N. Lau
  • Patent number: 8778155
    Abstract: A cartridge-based bio-separation system configured to utilize a pen shaped bio-separation cartridge that is easy to assemble and use with no moving parts and that has an integrated reagent (separation buffer) reservoir. The cartridge includes a body, defining an opening as a detection window for receiving external detection optics, at least one capillary column supported in the body, having a first end extending beyond a first end of the body, wherein the detection window exposes a section along the capillary column, to which the external optics are aligned through the detection window, and a reservoir attached to a second end of the body in fluid flow communication with a second end of the capillary column. The reservoir is structured to be coupled to an air pressure pump that pressurizes the gel reservoir to purge and fill the capillaries with buffer as the separation support medium.
    Type: Grant
    Filed: August 18, 2011
    Date of Patent: July 15, 2014
    Assignee: Bioptic, Inc.
    Inventors: Shou-Kuan Tsai, Varouj D. Amirkhanian
  • Publication number: 20140158537
    Abstract: Microfluidic methods of assaying molecule switching are provided. Aspects of the methods include microfluidically separating a sample containing the molecule of interest and then employing the resultant separation pattern to determine a switching characteristic of the molecule. Also provided are microfluidic devices, as well as systems and kits that include the devices, which find use in practicing embodiments of the methods. The methods, devices, systems and kits find use in a variety of different applications, such as analytical and diagnostic assays.
    Type: Application
    Filed: September 24, 2013
    Publication date: June 12, 2014
    Inventors: Amy E. Herr, Augusto M. Tentori, Alex J. Hughes
  • Patent number: 8734630
    Abstract: The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided.
    Type: Grant
    Filed: September 13, 2012
    Date of Patent: May 27, 2014
    Assignee: Applied Biosystems, LLC.
    Inventors: Karl O. Voss, Aldrich N. K. Lau
  • Patent number: 8728290
    Abstract: Disclosed is a novel microfluidic device enabling on-chip implementation of a two-dimensional separation methodology. Previously disclosed microscale immobilized pH gradients (IPG) are combined with perpendicular polyacrylamide gel electrophoresis (PAGE) microchannels to achieve orthogonal separations of biological samples. Device modifications enable inclusion of sodium dodecyl sulfate (SDS) in the second dimension. The device can be fabricated to use either continuous IPG gels, or the microscale isoelectric fractionation membranes we have also previously disclosed, for the first dimension. The invention represents the first all-gel two-dimensional separation microdevice, with significantly higher resolution power over existing devices.
    Type: Grant
    Filed: November 2, 2012
    Date of Patent: May 20, 2014
    Assignee: Sandia Corporation
    Inventors: Gregory J. Sommer, Anson V. Hatch, Anup K. Singh, Ying-Chih Wang
  • Patent number: 8603833
    Abstract: The present invention provides germania-silica-based sol-gel monoliths that form the solid-phase material for extraction, isolation, preconcentration, and separation of analytes. In one embodiment, the germania-silica-based sol-gel monolith can be used to coat surfaces of the inner walls of extraction and chromatographic columns. In a preferred embodiment, the sol-gel germania-silica monolith of the present invention is formed from hydrolysis and polycondensation of precursors comprising tetraethoxygermane, tetramethoxysilane, and polyethylene glycol. Also provided are methods of preparing the germania-silica-based sol-gel monolith of the present invention.
    Type: Grant
    Filed: June 3, 2011
    Date of Patent: December 10, 2013
    Assignee: University of South Florida
    Inventors: Abdul Malik, Erica B. Turner, Scott S. Segro
  • Publication number: 20130303734
    Abstract: The present disclosure relates to novel humanized antibodies able to inhibit tumor growth, as well as the amino and nucleic acid sequences coding for such antibodies. From one aspect, the disclosure relates to novel anti-JAM-A homogeneous humanized antibodies able to inhibit tumor growth. The disclosure also comprises the use of such antibodies as a drug for the preventive and/or therapeutic treatment of cancers, as well as compositions comprising such antibodies in combination with other anticancer compounds. The disclosure also relates to a method for the preparation of such novel humanized antibodies.
    Type: Application
    Filed: November 23, 2011
    Publication date: November 14, 2013
    Applicant: PIERRE FABRE MEDICAMENT
    Inventors: Liliane Goetsch, Nathalie Corvaia, Jean-François Haeuw, Cédric Bes, Alain Beck
  • Publication number: 20130248366
    Abstract: A method of amplifying nucleic acid includes providing a microfluidic device having a space therein. The space is filled with a gel medium. Reagents for carrying out a PCR reaction are supported within the matrix of the gel medium within the space. A nucleic acid containing sample is brought into contact with the gel medium. The sample having whole cells or cell lysate without any prior separation of the nucleic acid from other components of the cells. PCR amplification of nucleic acid from the sample is performed in the space using the PCR reagents.
    Type: Application
    Filed: September 21, 2011
    Publication date: September 26, 2013
    Applicant: THE UNIVERSITY OF HULL
    Inventors: Stephen John Haswell, Kirsty Jane Shaw, Peter Docker
  • Publication number: 20130213811
    Abstract: Systems and methods are provided for integrating the electrophoresis and blotting of samples. A capillary electrophoresis and blotting system allows concomitant electrophoretic separation and blotting to provide a rapid and simplified process. A microfluidic electrophoresis and blotting system provides electrophoretic separation in a microfluidic channel followed by electroblotting from the microfluidic channel to also provide a rapid and simplified process. These systems and methods can be used to assay smaller amounts of sample in less time than conventional processes, including conventional Western blotting techniques.
    Type: Application
    Filed: August 23, 2012
    Publication date: August 22, 2013
    Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN
    Inventors: Robert Kennedy, Gwendolyn Anderson
  • Patent number: 8512537
    Abstract: Various embodiments provide, for example, buffer compositions and/or sieving formulations useful in connection with electrophoresis instruments, such as capillary electrophoresis (CE) devices. In various embodiments, a buffer composition can include Bis-Tris, TAPS and/or TAPSO, and, optionally, a chelating agent, such as EDTA. Methods of separating samples containing bio-molecules, such as DNA or RNA, are also described.
    Type: Grant
    Filed: January 28, 2011
    Date of Patent: August 20, 2013
    Assignee: Applied Biosystems, LLC
    Inventors: Kevin J. Hacker, Karl O. Voss
  • Patent number: 8500980
    Abstract: A cost-effective multi-channel capillary gel-electrophoresis system for highly efficient, nucleic acid-based analysis, in particular HLA SSP (Human Leukocyte Antigen Sequence Specific Primer) typing applications. Twelve DNA samples are automatically injected, electrophoretically separated, detected and analyzed simultaneously by using a multiple usage and disposable multi-capillary gel-cartridge. Using commercially available DNA size markers as indicators, the system provides high resolving power with 12-channel separations in less than 1.5 minutes. The system can hold a total of 96 samples in a PCR plate that can automatically be analyzed within 25 minutes for a full plate of the HLA SSP kits.
    Type: Grant
    Filed: October 24, 2007
    Date of Patent: August 6, 2013
    Assignee: Qiagen Sciences, LLC
    Inventors: Varouj Amirkhanian, Ming-Sun Liu
  • Publication number: 20130078624
    Abstract: Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample.
    Type: Application
    Filed: September 26, 2011
    Publication date: March 28, 2013
    Applicant: Theranos, Inc., a Delaware Corporation
    Inventors: Elizabeth Holmes, Sunny Balwani
  • Publication number: 20130078625
    Abstract: Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample.
    Type: Application
    Filed: September 26, 2011
    Publication date: March 28, 2013
    Applicant: Theranos, Inc., a Delaware Corporation
    Inventors: Elizabeth Holmes, Joy Roy
  • Publication number: 20120267246
    Abstract: The present invention relates to novel methods and apparatus for improving the sensitivity of capillary zone electrophoresis (CZE). The invention particularly concerns devices comprising a channel that contains an in-line sol-gel column to concentrate samples being subjected to capillary zone electrophoresis, and the use of such devices to enhance the sensitivity of capillary zone electrophoresis.
    Type: Application
    Filed: April 20, 2012
    Publication date: October 25, 2012
    Inventors: Chitra Kumari Ratnayake, Michael Paul Henry
  • Patent number: 8293084
    Abstract: The present invention relates to methods for the identification of compounds in carbohydrate mixture compositions as well as the determination of carbohydrate mixture composition patterns, based on e.g. orthogonal cross determining migration time (indices) using capillary gel electrophoresis-laser induced fluorescence and identifying said carbohydrate components based on comparing said migration time (indices) with standard migration time (indices) from a database which data are preferably also orthogonal cross determined. In a further aspect, the present invention relates to a method for carbohydrate mixture composition pattern profiling, like glycosylation pattern profiling using capillary gel electrophoresis-laser induced fluorescence (CGE-LIF). In another aspect, the present invention refers to a system for an automated determination and/or identification of carbohydrates and/or carbohydrate mixture composition patterns (e.g.: glycosylation patterns).
    Type: Grant
    Filed: April 22, 2009
    Date of Patent: October 23, 2012
    Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.
    Inventors: Erdmann Rapp, Jana Schwarzer, Udo Reichl, Christian Bohne
  • Patent number: 8182746
    Abstract: An electrophoresis apparatus is generally disclosed for sequentially analyzing a single sample or multiple samples having one or more analytes in high or low concentrations. The apparatus comprises a relatively large-bore transport capillary which intersects with a plurality of small-bore separation capillaries and includes a valve system. Analyte concentrators, having antibody-specific (or related affinity) chemistries, are stationed at the respective intersections of the transport capillary and separation capillaries to bind one or more analytes of interest. The apparatus allows the performance of two or more dimensions for the optimal separation of analytes. The apparatus may also include a plurality of valves surrounding each of the analyte concentrators to localize each of the concentrators to improve the binding of one or more analytes of interest.
    Type: Grant
    Filed: October 31, 2007
    Date of Patent: May 22, 2012
    Assignee: Princeton Biochemicals, Inc.
    Inventor: Norberto A. Guzman
  • Patent number: 8163152
    Abstract: A cost-effective multi-channel capillary gel-electrophoresis system for highly efficient, high speed, high throughput, carbohydrate analysis. In one aspect of the present invention, a high-performance capillary electrophoresis analyzer system has been optimized for carbohydrate analysis application. The system uses a multiplexed/time-staggered fluorescence type detection mechanism, with an integrated fiber optic array-based technology and a novel disposable gel-cartridge. Twelve carbohydrate samples are automatically injected, electrophoretically separated, detected and analyzed simultaneously by using a multiple usage and disposable multi-capillary gel-cartridge. Using commercially available labeling agent such as APTS (representing 8-Aminopyrene-1,3,6-trisulfonic acid, trisodium salt) as an indicator, the capillary electrophoresis-based carbohydrate system/analyzer provides high resolving power in 2-15 minutes of separations.
    Type: Grant
    Filed: November 9, 2007
    Date of Patent: April 24, 2012
    Assignee: Qiagen Sciences, LLC
    Inventors: Varouj Amirkhanian, Ming-Sun Liu, Andras Guttman
  • Publication number: 20120043208
    Abstract: A multi-channel gel electrophoresis apparatus for efficiently collecting molecules isolated by gel electrophoresis so they can be further analyzed, identified, or used as reagents or medications. The apparatus using a novel “tagless” strategy that combines multi-dimensional separation of endogenous complexes with mass spectrometric monitoring of their composition. In this procedure, putative protein complexes are identified based on the co-migration of collections of polypeptides through multiple orthogonal separation steps. A majority of E. coli proteins are shown to remain in stable complexes during fractionation of a crude extract through three chromatographic steps.
    Type: Application
    Filed: July 5, 2011
    Publication date: February 23, 2012
    Applicant: The Regents of the University of California
    Inventors: Jian Jin, Mark D. Biggin, Robert A. Nordmeyer, Ming Dong, Earl W. Cornell, Megan Choi, Halina Ewa Witkowski, Bong-Gyoon Han, Robert M. Glaeser
  • Publication number: 20120015355
    Abstract: A size standard, kit includes a size standard, method of defining a size standard and method of analysis using a size standard. The size standard is intended to include size standard elements which have a size greater than and/or less than and/or different from the components of a sample which are to be sized. This means that the same characteristic unit, such as a dye, can be used to label the component and the size standard. A further characteristic unit, from amongst a limited number of such characteristic units is liberated from use only on size standards for use on components. The method is therefore particularly useful in multiplex amplification of STRs.
    Type: Application
    Filed: June 29, 2009
    Publication date: January 19, 2012
    Applicant: FORENSIC SCIENCE SERVICE LIMITED
    Inventors: Valerie Carol Tucker, Pieris Koumi, Andrew Hopwood, Keith Elliot
  • Patent number: 8097140
    Abstract: A method for collecting an analyte species from a sample is provided, the method of collection potentially being supplemented to give a method of preparing a sample for analysis and/or a method of analysis. The method including providing part of a sample in a substrate, causing the sample to migrate to an interface between the substrate and a second substrate due to the action of an electrical potential difference, the electrophoretic velocity of the analyte species of the second substrate being balanced by or exceeded by the bulk flow velocity of the second substrate and the bulk flow velocity of the second substrate being in an opposing direction to the electrophoretic velocity of the analyte species in the second substrate. In this way substantial concentration of the analyte species at the interface is provided. Subsequently the species can be conveyed away from the interface for further preparation and/or analysis.
    Type: Grant
    Filed: June 8, 2007
    Date of Patent: January 17, 2012
    Assignee: Forensic Science Service Ltd.
    Inventors: Silvia Valussi, Andreas Manz
  • Publication number: 20120000780
    Abstract: Linear acrylamide copolymer compounds which can comprise monomeric components comprising at least one N-substituted moiety capable of physical cross-linking, and related compositions and methods of use.
    Type: Application
    Filed: September 12, 2011
    Publication date: January 5, 2012
    Applicant: Northwestern University
    Inventors: Thomas N. Chiesl, Annelise E. Barron
  • Publication number: 20120004434
    Abstract: The present invention provides germania-silica-based sol-gel monoliths that form the solid-phase material for extraction, isolation, preconcentration, and separation of analytes. In one embodiment, the germania-silica-based sol-gel monolith can be used to coat surfaces of the inner walls of extraction and chromatographic columns. In a preferred embodiment, the sol-gel germania-silica monolith of the present invention is formed from hydrolysis and polycondensation of precursors comprising tetraethoxygermane, tetramethoxysilane, and polyethylene glycol. Also provided are methods of preparing the germania-silica-based sol-gel monolith of the present invention.
    Type: Application
    Filed: June 3, 2011
    Publication date: January 5, 2012
    Applicant: University of South Florida
    Inventors: Abdul Malik, Erica B. Turner, Scott S. Segro
  • Publication number: 20120000779
    Abstract: Provided herein is a surface acoustic wave (“SAW”) sensor device including an isolation component of a target biomolecule. A sample containing the target biomolecule is separated by its size using electrophoresis, and sequentially reacts with a SAW sensor. In other words, the device is capable of detecting the target biomolecule by separating biomolecules using electrophoresis, and applying the separated biomolecules to the SAW sensor.
    Type: Application
    Filed: December 21, 2010
    Publication date: January 5, 2012
    Applicants: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION, SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Hun Joo LEE, Soo Suk LEE, Mi Jeong SONG, Kyung Yeon HAN, Jae Ho KIM
  • Publication number: 20110263439
    Abstract: This application discloses SNPs capable of predicting increased or decreased risk of developing late onset Alzheimer's disease.
    Type: Application
    Filed: March 11, 2011
    Publication date: October 27, 2011
    Applicant: Translational Genomics Research Institute
    Inventors: Matt HUENTELMAN, Eric M. Reiman
  • Publication number: 20110250641
    Abstract: A compound of the formula: wherein R1 is independently selected from C1-C10 alkyl or substituted alkyl, R2 is independently selected from the group consisting of —H and C1-C6 alkyl or substituted alkyl, Y? is an anion, m is an integer from 1 to 8 and n is an integer from 1 to 8 is described. Methods of making and using the compound are also described. The compound may comprise a zwitterionic acid-labile surfactant suitable for purification and identification techniques used in proteomics.
    Type: Application
    Filed: April 7, 2011
    Publication date: October 13, 2011
    Inventors: Matthew Jacob Powell, Trust Tariro Razunguzwa, George Augustine O'Doherty, Miaosheng Li
  • Patent number: 8030092
    Abstract: An electrophoresis apparatus is generally disclosed for sequentially analyzing a single sample or multiple samples having one or more analytes in high or low concentrations. The apparatus comprises a relatively large-bore transport capillary which intersects with a plurality of small-bore separation capillaries and includes a valve system. Analyte concentrators, having antibody-specific (or related affinity) chemistries, are stationed at the respective intersections of the transport capillary and separation capillaries to bind one or more analytes of interest. The apparatus allows the performance of two or more dimensions for the optimal separation of analytes. The apparatus may also include a plurality of valves surrounding each of the analyte concentrators to localize each of the concentrators to improve the binding of one or more analytes of interest.
    Type: Grant
    Filed: November 17, 2006
    Date of Patent: October 4, 2011
    Assignee: Princeton Biochemicals, Inc.
    Inventor: Norberto A. Guzman
  • Patent number: 8007725
    Abstract: An electrophoresis apparatus is generally disclosed for sequentially analyzing a single sample or multiple samples having one or more analytes in high or low concentrations. The apparatus comprises a relatively large-bore transport capillary which intersects with a plurality of small-bore separation capillaries and includes a valve system. Analyte concentrators, having antibody-specific (or related affinity) chemistries, are stationed at the respective intersections of the transport capillary and separation capillaries to bind one or more analytes of interest. The apparatus allows the performance of two or more dimensions for the optimal separation of analytes. The apparatus may also include a plurality of valves surrounding each of the analyte concentrators to localize each of the concentrators to improve the binding of one or more analytes of interest.
    Type: Grant
    Filed: November 17, 2006
    Date of Patent: August 30, 2011
    Assignee: Princeton Biochemicals, Inc.
    Inventor: Norberto A. Guzman
  • Patent number: 7993507
    Abstract: The present invention is related to a method of separation of compounds by electrophoresis in which the compounds such as genes, proteins, etc. may be analyzed very precisely as samples are introduced directly into the separation tubes of the chip at the collection site, and therefore, it is not necessary to have separate fluid paths or individual sample storing apparatus that have been necessary for the conventional electrophoresis; it is easy to make the chips as the structure of the chip becomes extremely simple, and high-density arrangement of the separation tubes is enabled; and further, the compounds such as genes, proteins, etc. may be analyzed very precisely without interference in the storage tubs by using a non-polar solvent as the solvent of the sample storage tub.
    Type: Grant
    Filed: November 16, 2005
    Date of Patent: August 9, 2011
    Assignee: Korea Research Institute of Standards and Science
    Inventors: Sang-Ryoul Park, In-Chul Yang, Young-Ho Kim
  • Publication number: 20110165579
    Abstract: Described is a method of polymerase chain reaction (PCR) analysis of polymorphisms in the 16s-23s intergenic spacer region of Clostridium difficile (“PCR ribotyping of Clostridium difficile”) comprising the use of a 16s primer corresponding to bases 1450 to 1501 of the 16s ribosomal RNA gene of C. difficile and a 23s primer corresponding to bases 1 to 50 of the 23s ribosomal RNA gene of C. difficile, characterized in that the PCR is performed with a labeled 16s primer and an unlabeled 23s primer and that the DNA molecules provided by the PCR are separated by size using capillary gel electrophoresis.
    Type: Application
    Filed: September 9, 2009
    Publication date: July 7, 2011
    Applicant: Oster-Reichische Agentur Fur Gesundheit Und Emahrungssichertheit GMBH
    Inventor: Alexander Indra
  • Publication number: 20110143347
    Abstract: The present invention relates to a DNA profiling assay comprising the following steps, providing a sample to be analyzed, providing reagents, enzyme and primer oligonucleotides which are necessary for simultaneous polymerase chain reaction amplification of at least 20 loci, amplifying the loci, detecting the amplification products, wherein the amplification products and the loci to be amplified are characterized by the following features, each locus to be amplified is characterized by at least one deletion-insertion polymorphism known to be present in the population, wherein the two alleles from each locus differ in size by more than 2 nucleotides and less than 100 nucleotides, a first set of at least two amplification products ranging in size from about 20 nucleotides to about 300 nucleotides stemming from at least two different loci carries a first label, a second set of at least two amplification products ranging in size from about 20 nucleotides to about 300 nucleotides stemming from at least two differen
    Type: Application
    Filed: April 24, 2009
    Publication date: June 16, 2011
    Applicant: BIOTYPE GMBH
    Inventors: Manja Bohme, Jorg Gabert, Werner Brabetz
  • Patent number: 7955575
    Abstract: A microfluidic device comprising a set of one or more, preferably more than 5, covered microchannel structures manufactured in the surface of a planar substrate. The device is characterized in that a part surface of at least one of the microchannel structures has a coat exposing a non-ionic hydrophilic polymer. The non-ionic hydrophilic polymer is preferably attached covalently directly to the part surface or to a polymer skeleton that is attached to the surface.
    Type: Grant
    Filed: December 11, 2000
    Date of Patent: June 7, 2011
    Assignee: Gyros Patent AB
    Inventors: Helene Derand, Anders Larsson, James Van Alstine
  • Publication number: 20110031120
    Abstract: A gel composition for use in electrophoresis analysis of proteins, characterised in that the gel composition includes a polar solvent as an additive, wherein the polar solvent is one of either an alcohol or a formamide derivative. Methods of use, methods of manufacture and apparatus including the gel composition described hereinabove are also provided.
    Type: Application
    Filed: January 26, 2009
    Publication date: February 10, 2011
    Applicant: LAB 901 LIMITED
    Inventors: Adam Inche, Helen McNeill, Kenneth Macnamara
  • Patent number: 7862699
    Abstract: Matrix polymers and dynamic coating polymers, compositions thereof and related methods, systems and apparatus for microchannel separation.
    Type: Grant
    Filed: November 1, 2006
    Date of Patent: January 4, 2011
    Assignee: Northwestern University
    Inventors: Annelise E. Barron, Cheuk Wai Kan, Christopher P. Fredlake
  • Publication number: 20100300880
    Abstract: The present invention relates to a method for separating the components of a mixture M by electrophoresis in a single capillary, wherein: (A) the compounds of the mixture M are introduced and migrate inside the capillary according to the capillary electrophoresis technique; (B) a fraction F of the compounds separated in step (A) is isolated in the capillary and said isolated fraction F is forced to migrate towards the vicinity of one end of the capillary while maintaining this fraction inside the capillary; (C) upon the application of a pressure difference between the ends of the capillary, a second separation medium MS2 different from the first separation medium MS1 of step (A) is introduced into the capillary through the end of the capillary in the vicinity of which the fraction F is located, whereby the fraction F migrates to the other end of the capillary while filling the capillary with the separation medium MS2; and (D) an capillary electrophoresis is carried out in the capillary thus filled with the se
    Type: Application
    Filed: October 15, 2007
    Publication date: December 2, 2010
    Inventors: Hervé Cottet, Suzanne Anouti, Jean-Philippe Biron, Odile Vendenabeele-Trambouze
  • Patent number: 7828948
    Abstract: Disclosed herein are methods and devices for preconcentrating and separating analytes such as proteins and polynucleotides in microchannels. As disclosed, at least one size-exclusion polymeric element is adjacent to processing area or an assay area in a microchannel which may be porous polymeric element. The size-exclusion polymeric element may be used to manipulate, e.g. concentrate, analytes in a sample prior to assaying in the assay area.
    Type: Grant
    Filed: September 29, 2006
    Date of Patent: November 9, 2010
    Assignee: Sandia Corporation
    Inventors: Anson Hatch, Anup K. Singh, Amy E. Herr, Daniel J. Throckmorton
  • Publication number: 20100273178
    Abstract: A method for protein identification in complex mixtures that utilizes affinity selection of constituent proteolytic peptide fragments unique to a protein analyte. These “signature peptides” function as analytical surrogates. Mass spectrometric analysis of the proteolyzed mixture permits identification of a protein in a complex sample without purifying the protein or obtaining its composite peptide signature.
    Type: Application
    Filed: February 1, 2010
    Publication date: October 28, 2010
    Applicant: Purdue Research Foundation
    Inventor: Fred E. Regnier
  • Patent number: RE43122
    Abstract: The present invention provides an on-chip packed reactor bed design that allows for an effective exchange of packing materials such as beads at a miniaturized level. The present invention extends the function of microfluidic analysis systems to new applications including on-chip solid phase extraction (SPE) and on-chip capillary electrochromatography (CEC). The design can be further extended to include integrated packed bed immuno- or enzyme reactors.
    Type: Grant
    Filed: June 22, 2010
    Date of Patent: January 24, 2012
    Assignee: The Governors of the University of Alberta
    Inventors: D. Jed Harrison, Richard Oleschuk, Loranelle L. Lockyear, Cameron Skinner, Paul Li