Abstract: The invention relates to a novel biomimetic affinity purification material and its application in the purification of chitosanase, which belongs to the field of industrial biotechnology. The affinity ligand for the biomimetic affinity material is chitodisaccharides, the connecting arm is cyanuric chloride, and the base medium is epoxy-activated Sepharose™ 6B. The desorption constant (Kd) and the theoretical maximum adsorption capacity (Qmax) of the biomimetic affinity material are 24.2 ?g/mL and 24.1 mg/g, respectively. Using the above biomimetic affinity material, a chitosanase biomimetic affinity purification method is established, which can produce high-purity chitosanase with high efficiency and low cost, and has good industrial application potential.

Abstract: Provided are methods for producing a separation medium, where the method includes exposing a separation medium precursor solution to light from a light source through a photomask that includes a region with varied light transmittance to produce the separation medium. Systems that find use in performing the methods, microfluidic devices that include the separation medium, as well as methods of using the microfluidic devices, are also provided. Embodiments of the present disclosure find use in a variety of different applications, including detecting whether an analyte is present in a fluid sample.

Abstract: Methods and compositions are provided for preparing a biological specimen for microscopic analysis. These methods find many uses, for example in medicine and research, e.g., to diagnose or monitor disease or graft transplantation, to study healthy or diseased tissue, to screen candidate agents for toxicity and efficacy in disease modification. Also provided are reagents, devices, kits and systems thereof that find use in practicing the subject methods.

Abstract: Nanocomposite polymeric hydrogels comprising polyacrylamide (PAAm) formulated in combination with magnetically-susceptible anisotropic microparticles are described, as are method of making and using said hydrogels.

Abstract: Polyacrylamide gels that offer high resolution as electrophoretic media for protein separations and an improved resistance to hydrolysis upon storage are made by including either taurine, asparagine, or both as an ampholyte, in combination with either tris(hydroxymethyl)-aminomethane or bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane as a buffer, plus other conventional components.

Abstract: Separation methods based on electrophoresis or chromatography that use a stationary phase including fibril cellulose.

Abstract: The invention relates to graft copolymers, their preparation, and compositions, such as electrophoresis separation media, containing the same; also to ultra-high molecular weight poly(N,N-dimethylacrylamide) (“poly(DMA)”) polymers, their preparation, and compositions, such as electrophoresis separation media, containing the same; and more particularly to supports, such as capillaries, containing these polymers and methods for separating biomolecules, especially polynucleotides, using capillary electrophoresis. The graft copolymers can be prepared by, e.g., grafting polyacrylamide units onto a poly(DMA) backbone. Separation media comprising such graft copolymers or ultra-high molecular weight poly(DMA) polymers yield superior performance in the analysis and separation of biomolecules by capillary electrophoresis.

Abstract: A method for fixing a supercoiled DNA consists in deposing a supercoiled DNA sample on the surface of a porous polymer film, in fixing said supercoiled DNA therein by a passive diffusion, in obtaining a support by the inventive method and in using said support for analyzing the DNA distribution.

Abstract: The invention relates to compositions and methods of using electrophoresis separation matrices. The invention provides nano-particle comprising separation matrices having increased conductivity at low voltage.

Abstract: The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided.

Abstract: The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies thereby facilitating rapid isolation and purification of recombinant proteins. The invention further provides methods for producing protein molecular weight ladders for us in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.

Abstract: Gels, such as polyacrylamide gels, are provided that include linear polyacrylamide in the stacking gel. Native gels that include linear polyacrylamide in the stacker can be used to separate biomolecular complexes, such as protein complexes. Gel cassettes in which the gap width between front and back plates does not vary by more than 5% at the upper edge of the cassette are also provided. The gel cassettes can be used for electrophoretic separation of proteins and protein complexes on native gels, such as native gels that include linear polyacrylamide in the stacker. The native gels can have multiple wells for electrophoresing at least one sample and/or at least one molecular weight standard.

Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

Abstract: According to the present invention, a substituted aromatic compound represented by the following general formula (I) is provided. In general formula (I), A1, A2, and A3 each independently represent an aryl group substituted by a hydrophilic group.

Abstract: A pre-packaged electrophoresis cassette (1), the cassette (1) comprising a gel layer (2) and a buffer solution layer (3), the gel layer comprising a first polymer made from a monomer and a cross-linker, and being in contact with the buffer solution layer to form a gel-buffer interface (4) for receiving a sample which is to undergo electrophoresis. The gel and/or the buffer solution are such that absorption of water by the gel layer from the buffer solution layer is inhibited, thereby maintaining the performance capabilities of the electrophoresis cassette during storage.

Abstract: A technical measure for gel electrophoresis shaping that can make the gel electrophoresis generate no bubbles including in the gel, and can make a gradient gel being more accurate and more stable in quality. When liquid gel enters a collecting trough under continuous driving of a roller in the collecting trough to be injected into a carrier sheet set from a gel output port and a narrow seam, former injected liquid gel can be pushed upwards by latter injected liquid gel, and an accomplished product of gel can be obtained. If the collecting trough is input with two liquid basic materials, the liquid gel continuously output into the carrier sheet set can have a gradient.

Abstract: Embodiments herein concern systems, methods, compositions and apparatus for detection and/or determination of the presence and/or concentration of target molecules in a sample. In certain embodiments, target molecules can be separated and analyzed using non-gel electrophoresis technologies.

Abstract: A degradable polyacrylamide gel for analyzing or separating at least one macromolecule in a sample using electrophoresis includes a polyacrylamide cross-linked with at least one degradable cross-linker having a ketal or acetal group with the formula (I), wherein R1 and R2 are the same or different and are hydrogen, an alkyl, or a substituted alkyl.

Abstract: A technical measure for gel electrophoresis shaping that can make the gel electrophoresis generate no bubbles including in the gel, and can make a gradient gel being more accurate and more stable in quality. When liquid gel enters a collecting trough under continuous driving of a roller in the collecting trough to be injected into a carrier sheet set from a gel output port and a narrow seam, former injected liquid gel can be pushed upwards by latter injected liquid gel, and an accomplished product of gel can be obtained. If the collecting trough is input with two liquid basic materials, the liquid gel continuously output into the carrier sheet set can have a gradient.

Abstract: The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided.

Abstract: The invention is drawn to composite agarose/acrylamide compositions and gels. In particular it relates to gels for the separation of molecules, particularly macromolecules such as proteins. The invention is also directed to the preparation of composite gels, the separation of molecules by techniques such as electrophoresis using such gels, and the transfer of proteins from such gels to a transfer membrane using an immunoblot transfer gel.

Abstract: Disease specific markers, in particular cancer markers, can be detected by electrophoretically separating proteins and protein complexes from a biological sample on a protein binding polymeric membrane in a low conductivity, water-miscible organic solvent buffer. Electrophoretic separation profiles representing different diseases can be produced, and used in the diagnosis or prognosis of these diseases.

Abstract: The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided.

Abstract: Polymeric compounds and related methods and apparatus, as can be used in a wide range of RNA and DNA separations.

Abstract: The invention provides dry compositions for preparing and loading a sample on a gel for electrophoretic separation. The dry compositions preferably include a tracking dye and a sedimenting agent selected from a five-carbon polyol (e.g., ribitol, arabitol, or xylitol), iso-erythritol, maltitol, and saccharine. Methods for making and using, as well as kits comprising the disclosed compositions, are also provided.

Abstract: The invention relates to hydrogels containing water and polyethylene glycol-dimethacrylates in a polymerized form. The polymethacrylate blocks are so short that they form no proper phase. The invention further relates to methods for producing said hydrogels. The inventive hydrogels are provided with reduced haze and are used as materials for contact lenses, electrophoresis gels, membrane materials, and sound-absorbing materials.

Abstract: Disclosed are thermoresponsive microparticle composite hydrogels comprising poly(N-isopropyl acrylamide) and polyacrylamide, and methods regarding their manufacture and their use. The present invention provides in one aspect a thermoresponsive microparticle hydrogel, wherein the matrix morphology is controllably and selectively altered by incorporation of thermoresponsive nano/micro-particles. The particles are preferably poly(N-isopropyl acrylamide) particles. The present invention also provides methods of making and using such hydrogels.

Abstract: The present disclosure provides isotopically substituted compounds of the formula (II): wherein T, U, V, W, X, Y, Z, R0, R3, R4, R5 and R6 are as defined in the detailed description. The method for detection and quantification using the same is also disclosed.

Abstract: Disclosed is a precast polyacrylamide gel for use in gel electrophoresis, comprising polyacrylamide and an aqueous Tris 0.04 M to 0.15 M solution, at least one first ampholyte exhibiting an isoelectric point (pI) of from 5.4 to 6.4 at a total concentration of from 0.01 M to 0.4 M; and at least one second ampholyte exhibiting an isoelectric point (pI) of from 2.5 to 3.5 to adjust the pH to between 5.5 and 7.5 at the temperature of 25° C. The precast gels can be used for electrophoresis of oligopeptides, polypeptides, oligonucleotides, and polynucleotides under denaturating or nondenaturing conditions, and exhibit long shelf-life.

Abstract: An electrophoresis gel has a colored or pigmented loading area at one edge having a plurality of sample wells for receiving samples, the loading area extending beyond the ends of the wells so that the wells can be visually differentiated from the surrounding colored material in the loading area. The remainder of the gel is transparent or substantially transparent.

Abstract: This invention provides a reagent for protein electrophoresis, an electrophoresis gel or buffer composition containing the reagent, and a protein separation method and an electrophoresis kit using the reagent and the composition.

Abstract: A gel and buffer system for gel electrophoresis wherein separation occurs at neutral pH.

Abstract: The present invention relates to electrophoresis and in particular low fluorescent electrophoretic supports for hydrogels used for separation of fluorescence labelled biomolecules. More particularly, the invention relates to use of a polymer having the following formula: wherein n=0-100 000 m=0-100 000 R1, R2, R3 and R4=H, F, Cl, Br, I, methyl groups or non-aromatic hydrocarbon chains (optionally containing branches or cyclic structures) such as ethyl, ethenyl, propyl, isopropyl, propenyl, butyl, branched butyl, butenyl, cyclobutyl, pentyl, branched pentyl, pentenyl, cyclopentyl, hexyl, branched hexyl, cyclohexyl; X, Y=methylene groups or non-aromatic hydrocarbon chains (optionally containing branches or cyclic structures) such as ethylene, ethenylene, propylene, isopropylene, propenylene, butylene, branched butylene, butenylene; Y can optionally be absent as a low fluorescent support film of an electrophoretic hydrogel for slab gel electrophoresis.

Abstract: The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells.

Abstract: A degradable polyacrylamide gel for analyzing or separating at least one macromolecule in a sample using electrophoresis includes a polyacrylamide cross-linked with at least one degradable cross-linker having a ketal or acetal group with the formula (I), wherein R1 and R2 are the same or different and are hydrogen, an alkyl, or a substituted alkyl.

Abstract: It is intended to provide a method for easily detecting a phosphorylated peptide (protein) in a test sample by using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) which has been conventionally employed in analyzing a protein; a polyacrylamide gel for electrophoresis to be used in the method; a method of producing the gel; and a synthesizing intermediate in producing the gel. The polyacrylamide gel for electrophoresis to be used in the method of the invention is characterized in that at least a part of the structure thereof has a structure represented by the following formula (I); wherein M2+represents a transition metal ion; and X represents a linker group.

Abstract: A gel composition for use in electrophoresis analysis of proteins, characterised in that the gel composition includes a polar solvent as an additive, wherein the polar solvent is one of either an alcohol or a formamide derivative. Methods of use, methods of manufacture and apparatus including the gel composition described hereinabove are also provided.

Abstract: Matrix polymers and dynamic coating polymers, compositions thereof and related methods, systems and apparatus for microchannel separation.

Abstract: The invention provides a method of separating a complex comprising a first agent bound to a second agent from unbound agents comprising (a) preparing a gel comprising a gel matrix and a neutral solute, (b) placing one or more samples comprising the complex and unbound agents on the gel, and (c) applying an electrophoresis voltage across the gel and separating the complex from the unbound agents. The invention also provides methods of separating a complex comprising a first agent bound to a second agent from unbound agents by chromatography and capillary electrophoresis.

Abstract: Methods for detecting one or more analytes, such as a protein, in a fluid path are provided. The methods include resolving, immobilizing and detecting one or more analytes in a fluid path, such as a capillary. Also included are devices and kits for performing such assays.

Abstract: Provided is a precast polyacrylamide gel for use in gel electrophoresis, having a shelf-life of at least 12 months. The gel comprises a sulfonated tertiary or secondary amine having a pKa of 6.6-8.0, ampholytes exhibiting pI between 5.4 to 6.4, Tris, and hydrochloric acid to adjust the pH to between 5.5 and 7.5.

Abstract: A series of low molarity conductive media based on non-buffering univalent cations, such as sodium chloride-sodium acetate (SCA), sodium boric acid (SB), lithium boric acid, and lithium acetate mitigate the “runaway” positive feedback heating loop produced by conventional media containing biological amine buffers and permit improved DNA electrophoresis under the conditions of low salt concentration. These media serve well in ultra-fast DNA electrophoresis and in high-resolution separations of RNA and DNA fragments.

Abstract: The present invention describes a method of preparation of a permanent neutral wall coating made of thermally immobilized polysaccharides. The coating suppresses electroosmotic flow and adsorption on the wall under acidic, neutral, and basic conditions in capillary electrophoresis.

Abstract: The invention relates to a method of preparing a gel for use in electrophoresis, which method comprises: (a) providing a mixture comprising a solvent, agarose or a derivative thereof, one or more vinyl monomers, and a polymerisation initiator, the initiator forming a redox system with agarose or the derivative thereof; (b) exposing the mixture to polymerisation conditions to graft-polymerise the one or more monomers onto the agarose or derivative thereof; and (c) allowing the mixture to form a gel.

Abstract: An electrophoresis gel matrix is provided for conducting proteomics, in particular the steps of separating and/or identifying proteins. The matrix comprises at least one chemical reagent being at least one of a denaturing, buffering, disaggregating, reducing, staining or dissolving reagent, and a volume of dimethylsulfoxide. The use of such a matrix, which is provided to be filled into an electrophoresis capillary being part of a microfluidic device for carrying out proteomics, leads to an enhanced resolution of proteins. The matrix is prepared adding a volume of dimethylsulfoxide ranging from 4% to 15%, preferably from 5% to 10%, most preferably from 6% to 8% with respect to the total volume of chemical reagents being comprised in the matrix. A method is provided for preparation of the matrix for conducting proteomics and a method is conducted to carry out proteomics by the use of this matrix.

Abstract: Disclosed is a system or matrix approach for determining compatibility of a pharmaceutically active substance, such as small molecule drug candidate, therapeutic proteins, peptides, vaccines or RNAi, with materials used in the research and development of pharmaceuticals, including plastics, polymers, resins, rubbers, elastomers, glass and steel.

Abstract: Polyacrylamide gels that offer high resolution as electrophoretic media for protein separations and an improved resistance to hydrolysis upon storage are made by including either taurine, asparagine, or both as an ampholyte, in combination with either tris(hydroxymethyl)-aminomethane or bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane as a buffer, plus other conventional components.

Abstract: An electrophoresis gel has a colored or pigmented loading area at one edge having a plurality of sample wells for receiving samples, the loading area extending beyond the ends of the wells so that the wells can be visually differentiated from the surrounding colored material in the loading area. The remainder of the gel is transparent or substantially transparent.

Abstract: The invention provides an electrophoresis cassette, methods for making the electrophoresis cassette, and method of fractionating analytes from a sample based upon electrophoretic mobility in a single application of the sample to an electrophoretic system.

Abstract: The present disclosure provides methods, devices, systems and compositions for nucleic acid separation and/or purification. In some embodiments, nucleic acids from about 10 nucleotides to about 150 nucleotides may be separated and/or purified in seconds to minutes. A system for purifying a nucleic acid within seconds to minutes may include: a fractionator having a housing, a first electrode, a second electrode spaced away from the first electrode, and a lower buffer chamber proximal to the second electrode; and a pre-cast gel cartridge having an upper buffer chamber and an elongate polyacrylamide gel, wherein the upper buffer chamber is in fluid communication with one end of the polyacrylamide gel, the lower buffer chamber is in fluid communication with the other end of the elongate polyacrylamide gel, the first electrode is in electrical communication with the upper buffer chamber, and the second electrode is in electrical communication with the lower buffer chamber.