With Detailed Detection System (e.g., Including A Light Source And A Camera, Etc.) Patents (Class 204/603)
  • Patent number: 10156537
    Abstract: A sensor for detecting a liquid in a fluid channel of a microfluidic component and to a microfluidic component having such a sensor. The sensor includes an electrode arrangement having a transmitting electrode, a receiving electrode and a first shielding electrode, which are arranged in a coplanar manner on a plane and can be positioned above or below, adjacent to the fluid channel, wherein the transmitting electrode and the receiving electrode are capacitively coupled in that they each have an adjacently arranged edge having a dielectric therebetween.
    Type: Grant
    Filed: July 16, 2015
    Date of Patent: December 18, 2018
    Assignee: FRAUNHOFER-GESELLSCHAFT ZUR FÖRDERUNG DER ANGEWANDTEN FORSCHUNG E.V.
    Inventors: Almuth Hoffmann, Michael Bassler, Karin Potje-Kamloth, Knut Welzel, Matthias Besold
  • Patent number: 10141707
    Abstract: A fiber laser system includes a fiber laser connector having a housing to terminate a fiber that generates a laser beam. A chamber extends internally along a length of the housing. A light trap includes a plurality of threads formed along a wall of the chamber to trap light reflected back to the fiber laser connector in response to an application of the laser beam to a workpiece.
    Type: Grant
    Filed: May 16, 2017
    Date of Patent: November 27, 2018
    Assignee: NLIGHT, Inc.
    Inventors: Scott R. Karlsen, Walter R. Sanders
  • Patent number: 10073024
    Abstract: A microfluidic device for detecting rare cells in a fluid sample comprises the rare cell and other cells. The microfluidic device comprises an inlet for receiving the fluid sample, a labyrinth channel structure in fluid communication with the inlet, and an outlet in fluid communication with the labyrinth channel structure for collecting the rare cells separated from the other cells in the fluid sample. The labyrinth channel structure comprises at least one channel through which the fluid sample flows. The at least one channel has a plurality of segments and a plurality of corners with each corner defined between adjacent segments. The presence of the plurality of corners induces separation of the rare cells from the other cells in the fluid sample as the rare cells move to a first equilibrium position within the at least one channel when a ratio of inertial lift forces (FZ) and Dean flow (FD) of the fluid sample is from 2 to 10.
    Type: Grant
    Filed: October 29, 2013
    Date of Patent: September 11, 2018
    Assignee: THE REGENTS OF THE UNIVERSITY OF MICHIGAN
    Inventors: Sunitha Nagrath, Hyeun Joong Yoon, Eric Lin, Max S. Wicha, Lianette Rivera Baez, Diane M. Simeone
  • Patent number: 9952169
    Abstract: A method of measuring biological sample properties and a biological sample property measuring apparatus is provided. A method of measuring biological sample properties includes disposing a biomaterial to contact a sensing unit, detecting a radio frequency (RF) signal flowing through the sensing unit, and obtaining an RF property indicator of the biomaterial based on the detected RF signal.
    Type: Grant
    Filed: October 13, 2015
    Date of Patent: April 24, 2018
    Assignees: Samsung Electronics Co., Ltd., Industry-Academic Cooperation Foundation, Yonsei University
    Inventors: Sang Uk Son, Duck Hwan Kim, In Sang Song, Seong Chan Jun, Ho Soo Park, Jea Shik Shin, Moon Chul Lee
  • Patent number: 9791630
    Abstract: An apparatus for discriminating bacteria types using optical scattering patterns is disclosed. The apparatus includes an optical fiber for transferring light emitted from a light source, a lens for controlling a width of the light received from the optical fiber, a linear polarizer for transmitting the light passing through the lens and a bacterial colony, and a capturing unit for capturing an optical scattering pattern of the light transmitted through the linear polarizer.
    Type: Grant
    Filed: August 1, 2016
    Date of Patent: October 17, 2017
    Assignee: Pukyong National University Industry-University Cooperation Foundation
    Inventors: Yongwook Lee, Junghwan Oh, Hyunwook Kang, Seungyun Nam, Youngmog Kim, Junhyeog Jeong
  • Patent number: 9733174
    Abstract: Low-cost and easily-operated microviscometer suitable for medical diagnosis clinical studies and other fluid tests. The equipment consists of a microchannel (2) formed by concatenated microchannels made by micro-manufacturing techniques, and a fluid column position detector inside the microchannel. The microchannels are open at one end and closed at the other end and are made of a single biocompatible material. When a liquid drop is put into the inlet of the microchannel (2), the fluid enters by capillary until the compressed air pressure equals the capillary pressure plus atmospheric pressure. The fluid transient movement from entering the channel until stopping at its balance position is analyzed thus obtaining as a result the viscosity and the capillary pressure of the liquid tested.
    Type: Grant
    Filed: July 5, 2012
    Date of Patent: August 15, 2017
    Assignees: COMISION NACIONAL DE ENERGIA ATOMICA, INIS BIOTECH LLC, CONSEJO NACIONAL DE INVESTIGACIONES CIENTIFICAS Y TECNICAS
    Inventors: Nadim Marcelo Morhell, Hernán Pastoriza
  • Patent number: 9683961
    Abstract: Disclosed are methods and systems for the simultaneous separation and detection of analytes such as anions and cations in a sample using electrophoresis, the method comprising injecting the sample into an electrophoresis system comprising two separation channels through a single sample injection port which is in fluid communication with the two separation channels, separating analytes such as the cations in a first of the two separation channels and simultaneously separation analytes such as the anions in a second of the two separation channels, and detecting the analytes separated in each of the separation channels.
    Type: Grant
    Filed: August 13, 2013
    Date of Patent: June 20, 2017
    Assignee: University of Tasmania
    Inventors: Michael C. Breadmore, Adam J. Gaudry, Rosanne M. Guijt
  • Patent number: 9588080
    Abstract: A nanosensor for detecting molecule characteristics includes a membrane having an opening configured to permit a charged carbon nanotube to pass but to block a molecule attached to the carbon nanotube. The opening is filled with an electrolytic solution. An electric field generator is configured to generate an electric field relative to the opening to drive the charged carbon nanotubes through the opening. A sensor circuit is coupled to the electric field generator to sense current changes due to charged carbon nanotubes passing into the opening, and to bias the electric field generator to determine a critical voltage related to a force of separation between the carbon nanotube and the molecule.
    Type: Grant
    Filed: August 15, 2013
    Date of Patent: March 7, 2017
    Assignee: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Binquan Luan, Ruhong Zhou
  • Patent number: 9533306
    Abstract: A single-sided continuous optoelectrowetting (SCOEW) device for manipulating droplets retained in a fluid over the SCOEW device with dynamic patterns of low intensity light, such as from a display screen, is described. A single pair of lateral electrodes are utilized for providing a lateral electric field bias, with transport motion controlled in response to projecting light through a photoconductive layer and dielectric layer adjacent to which droplets are retained. The device is configured for optically manipulating droplets having volumes spanning over five orders of magnitude, and can be configured to perform droplet dispensing, transport, splitting, merging, mixing and other droplet manipulation functions involving any of the above on a single sided surface.
    Type: Grant
    Filed: July 31, 2011
    Date of Patent: January 3, 2017
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Pei-Yu Chiou, Sung-Yong Park
  • Patent number: 9522520
    Abstract: A process and a laminating device for producing a microfluidic device are proposed, wherein a film is pressed onto a carrier under the effect of heat and thereby attached to it. During the attachment the film is lifted by the blowing in of gas on the carrier side to prevent it adhering in a non-attachment region and is vented on the remote side. The device is compressed between a holder and a thermode of the laminating device during the lamination. An elastic intermediate layer is arranged in each case between the device and the holder and between the device and the thermode, for evening out the contact pressure and obtaining uniform attachment of the film to the carrier.
    Type: Grant
    Filed: August 30, 2011
    Date of Patent: December 20, 2016
    Assignee: Boehringer Ingelheim Microparts GmbH
    Inventors: Dirk Kurowski, Oliver Paul
  • Patent number: 9523656
    Abstract: The invention provides methods and systems for ruggedizing a nucleic acid analyzing apparatus. The ruggedized apparatus can be used reliably and effectively in uncontrolled environments, such as, for example at a crime scene to collect and analyze forensic data, as well as in semi-controlled environments, such as, for example at a point of care location.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: December 20, 2016
    Assignee: NetBio, Inc.
    Inventors: Eugene Tan, Heung Chuan Lam, Gregory John Kellogg
  • Patent number: 9304334
    Abstract: A microfluidic panel including at least one substrate, one or more channels formed in the substrate, and fluid disposed within the one or more channels. The fluid is selected to store thermal energy and the microfluidic panel is adapted to convert the thermal energy into useable energy or condition the energy to adjust optical wavelength passband of the panel.
    Type: Grant
    Filed: January 21, 2014
    Date of Patent: April 5, 2016
    Assignee: PHOTRONICS, INC.
    Inventor: Christopher J. Progler
  • Patent number: 9168529
    Abstract: Systems and methods for air cooling a microfluidic device using confinement channels to isolate cooling air from exposed liquids are disclosed. The systems and methods may also thermally condition the cooling airflow for improved robustness of the microfluidic device. In one embodiment, the air cooling system includes a split-level cooling manifold including an inlet duct that directs cooling air to a microfluidic device and an outlet duct that directs air heated by the microfluidic device away from the microfluidic device. The temperature of cooling air may be measured. The cooling air may be preheated to a temperature that is higher than an expected ambient temperature. The temperature of the cooling air after being heated by a microfluidic device may be measured.
    Type: Grant
    Filed: August 31, 2011
    Date of Patent: October 27, 2015
    Assignee: Canon U.S. Life Sciences, Inc.
    Inventors: Johnathan S. Coursey, Kenton C. Hasson, Ben Lane, Eric Schneider
  • Patent number: 9120298
    Abstract: A microfluidic chip includes a thin biaxially-oriented polyethylene terephthalate (“BoPET”) film and a micro-channel in the BoPET film. A method for manufacturing a microfluidic chip includes coating UV epoxy on a first side of a BoPET film, placing the BoPET film on a first substrate with the first side facing the first substrate, curing the UV epoxy on the first side of the BoPET film to attach the BoPET film on the first substrate; forming at least one microfluidic pathway in the BoPET film, coating UV epoxy on a first side of a second substrate, placing the second substrate on the BoPET film with the first side of the second substrate facing a second side of the BoPET film, and curing the UV epoxy on the first side of the second substrate to attach the BoPET film to the second substrate. The microfluidic chip may be a multi-layered chip.
    Type: Grant
    Filed: September 16, 2013
    Date of Patent: September 1, 2015
    Assignee: FluxErgy, LLC
    Inventors: Tej Patel, Ryan Revilla, Matthew D'Ooge
  • Patent number: 9090666
    Abstract: An optical fiber (500) illuminates the bore (520) of a capillary tube (515) that is used for separating chemicals by capillary electrophoresis (CE). The fiber terminates in either two sloped regions (525) and a curved region (530) or two sloped regions (705) and a flat region (700). Light from these regions is focused on the bore of the capillary tube. Since the fiber is sized to illuminate the core of a CE capillary, it is larger than fibers used in telecommunications and its sloped regions are at angles that would be unsuitable for use in telecommunications. The relatively large diameter of the capillary permits efficient use of a light source (905).
    Type: Grant
    Filed: June 27, 2012
    Date of Patent: July 28, 2015
    Inventor: Tiansong Wang
  • Publication number: 20150107999
    Abstract: An apparatus for separating an analyte from a test sample, such as bacteria from blood components, based on their dielectric properties, localizing or condensing the analyte, flushing substantially all remaining waste products from the test sample, and detecting low concentrations of the analyte. The module array includes a plurality of microfluidic channels with connecting microfluidic waste channels for directing undesired material away from the analyte. An electric field is applied causing a positive dielectrophoretic force to the analyte to capture the analyte. The electric field is applied to at least one electrode having a plurality of concentric rings or concentric arcs extending radially outwards from a center point, electrically connected to a voltage source such that when voltage is applied to the at least one electrode, the concentric rings or concentric arcs alternate in voltage potential.
    Type: Application
    Filed: December 24, 2014
    Publication date: April 23, 2015
    Inventors: Monika Weber, Mark A. Reed
  • Publication number: 20150101931
    Abstract: The invention features the use of graphene, a one atom thick planar sheet of bonded carbon atoms, in the formation of artificial lipid membranes. The invention also features the use of these membranes to detect the properties of polymers (e.g., the sequence of a nucleic acid) and identify transmembrane protein-interacting compounds.
    Type: Application
    Filed: September 5, 2014
    Publication date: April 16, 2015
    Inventors: Slaven GARAJ, Daniel BRANTON
  • Publication number: 20150083597
    Abstract: A perforated metal oxide semiconductor (MOS) structure for single biomolecule, virus, or single cell detection is disclosed. The structure includes a nanochannel formed through a sensing region configured to allow a solution containing particles to pass through the perforated MOS sensor. First and second terminals are configured to measure electrical parameters representative of change of electrical characteristics of the solution as the particle passes through the perforated MOS structure.
    Type: Application
    Filed: September 26, 2014
    Publication date: March 26, 2015
    Applicant: Purdue Research Foundation
    Inventors: Saeed Mohammadi, Mojgan Sarmadi, Hossein Pajouhi
  • Patent number: 8986530
    Abstract: Embodiments of analysis systems, electrophoresis devices, and associated methods of analysis are described herein. In one embodiment, a method of analyzing a sample containing an electrolyte includes sequentially introducing a leading electrolyte, a sample electrolyte, and a trailing electrolyte into a extraction channel carried by a substrate. The extraction channel has a constriction in cross-sectional area. The method also includes applying an electrical field to separate components of the sample electrolyte into stacks and to concentrate the separated components by forcing the sample electrolyte to migrate through the constriction in the extraction channel. Thereafter, the applied electrical field is removed and the separated and concentrated components of the sample are detected in a detection channel carried by the substrate.
    Type: Grant
    Filed: April 12, 2012
    Date of Patent: March 24, 2015
    Assignee: Washington State University
    Inventors: Cornelius F. Ivory, Dan M. Leatzow
  • Patent number: 8968678
    Abstract: An apparatus and methods for concentrating samples for application to microfluidic devices are disclosed. The methods involve electrophoresing charged molecules from a high volume sample into a smaller volume. The analyte of interest can be a charged molecule or can be modified to be charged using, for example, one or more ionic moieties.
    Type: Grant
    Filed: June 28, 2012
    Date of Patent: March 3, 2015
    Assignee: Headway Technologies, Inc.
    Inventor: Celine Hu
  • Patent number: 8968540
    Abstract: A device for reading the sequence of a polymer, consisting of a first electrode and a second electrode, each electrode being functionalized with a reader molecule strongly bonded to the electrodes, but forming weak bonds with a molecule to be sequenced. In particular, the reader molecule is designed to form bonds with at least two points on the target molecule such that the target molecule is trapped between a first reader molecule on one electrode and a second reader molecule on the second electrode, with the overall size of the molecular complex being small enough to permit significant electric current to flow.
    Type: Grant
    Filed: October 6, 2009
    Date of Patent: March 3, 2015
    Assignee: Arizona Board of Regents, a Body Corporate of the State of Arizona Acting for and on Behalf of Arizona State University
    Inventors: Kevin Reinhart, Stuart Lindsay, Peiming Zhang
  • Patent number: 8961757
    Abstract: The present invention provides a device for analyzing the composition of a heteropolymer comprising a carbon nanotube through which the heteropolymer is driven by electrophoresis. The carbon nanotube also serves as one electrode in a reading circuit. One end of the carbon nanotube is held in close proximity to a second electrode, and each end of the carbon nanotube is functionalized with flexibly-tethered chemical-recognition moieties, such that one will bind one site on the emerging polymer, and the second will bind another site in close proximity, generating an electrical signal between the two electrodes when the circuit is completed by the process of chemical recognition.
    Type: Grant
    Filed: March 18, 2009
    Date of Patent: February 24, 2015
    Assignees: Arizona Board of Regents, a body corporate of the State of Arizona Acting for and on behalf of Arizona State University, The Trustees of Columbia University in the City of New York
    Inventors: Colin Nuckolls, Jinyao Tang, Stuart Lindsay, Jin He, Peiming Zhang, Kevin Reinhart
  • Patent number: 8961764
    Abstract: A DNA analyzer includes an interface for coupling a microfluidic chip to the DNA analyzer. The microfluidic chip includes a first separation channel for electrophoretic separation of DNA fragments in a first sample. Further, the DNA analyzer includes a first optical device. The first optical device includes an illuminating path and a detecting path. The illuminating path directs a first input light beam received from a light source to a first separation channel of the microfluidic chip. The first input light beam causes fluorescent labels attached on DNA fragments in the first separation channel to emit a first fluorescence light. The detecting path collects and directs the first fluorescent light to a first plurality of optical fibers. Further, the DNA analyzer includes a spectrometer configured to receive the first fluorescent light from the plurality of optical fibers and detect fluorescent components in the first fluorescent light.
    Type: Grant
    Filed: October 14, 2011
    Date of Patent: February 24, 2015
    Assignees: Lockheed Martin Corporation, ZyGEM Corporation, Ltd.
    Inventors: Peter Karl Trost, Michael E. Egan, Doug South, Brian E. Root, Orion N. Scott, James P. Landers
  • Patent number: 8961765
    Abstract: Plastic electrophoresis separation chips are provided comprising a plurality of microfluidic channels and a detection window, where the detection window comprises a thin plastic; and the detection window comprises a detection region of each microfluidic channel. Such chips can be bonded to a support provided an aperture is provided in the support to allow detection of samples in the electrophoresis chip at the thin plastic detection window. Further, methods for electrophoretically separating and detecting a plurality of samples on the plastic electrophoresis separation chip are described.
    Type: Grant
    Filed: August 8, 2012
    Date of Patent: February 24, 2015
    Assignee: NetBio, Inc.
    Inventors: Eugene Tan, Cheuk Wai Kan, Heung Chuan Lam
  • Publication number: 20150050721
    Abstract: The present invention provides a chip for analysis of a target substance that is compact and allows analysis of a target substance with less time and effort. The chip for analysis of a target substance includes a first flexible substrate 1, a second flexible substrate 2, and a third substrate 3. A flow channel-forming non-bonded area 11 is formed on a bonding surface of the first flexible substrate 1 and the second flexible substrate 2 in a band-like manner and an extraction chamber-forming non-bonded area 5 having a wider band width than the flow channel-forming non-bonded area 11 is formed at a part of the flow channel-forming non-bonded area 11.
    Type: Application
    Filed: January 23, 2013
    Publication date: February 19, 2015
    Inventors: Minoru Asogawa, Hisashi Hagiwara, Yoshinori Mishina, Yasuo Iimura
  • Publication number: 20150027893
    Abstract: A convenient technique allows detecting fluorescence emitted in the channel with a uniformly high detection sensitivity and a good reproducibility. On the front face side of a plate-like body 11, a groove-shaped introduction channel 12 and separation channel 14 are formed. In this micro-channel chip 10, on the inner faces of a groove constituting the separation channel 14 formed in a surface of the plate-like body 11, there is formed a fine particle layer 20 provided by sintering fine particles having an average particle diameter of 10 to 500 nm mainly composed of zinc oxide (ZnO) or titanium oxide (TiO2).
    Type: Application
    Filed: February 25, 2013
    Publication date: January 29, 2015
    Inventors: Yasuhisa Fujita, Haruo Takeshita, Junko Fujihara, Yutaka Fukui, Mari Tabuchi
  • Publication number: 20150008130
    Abstract: A separation module operates to fractionate or separate an analyte into fractions according to pI, i.e., pI bands, utilizing capillary isoelectric focusing (“CIEF”) within a first microchannel. The fractions are stacked to form plugs, the number of which is determined by a number of parallel second microchannels integrally connected to the first microchannel, into which the fractions are directed according to the buffer characteristics found in each of the individual microchannels. Within the microchannels the plugs are separated into proteins according to a different chemical property, i.e., “m/z,” utilizing capillary electrophoresis (“CE”).
    Type: Application
    Filed: September 25, 2014
    Publication date: January 8, 2015
    Inventors: Thomas Wayne Schneider, James N. Baraniuk
  • Publication number: 20150008124
    Abstract: Devices and methods for detecting the length of analytes and/or sequencing analytes are provided in which two or more electrical signals are obtained as an analyte traverses a fluidic channel. Detection of the relative position of probes hybridized to a biopolymer and/or the length of the analyte (e.g., a biopolymer) does not rely on the absolute time between detection events of a given electrical signal to determine a distance associated with the biopolymer. Instead, multiple signals are obtained (e.g., as functions of time) corresponding to a plurality of detector volumes at known locations along a fluidic channel through which the biopolymer passes, and the distances are determined from the multiple signals.
    Type: Application
    Filed: July 15, 2014
    Publication date: January 8, 2015
    Inventor: JOHN S. OLIVER
  • Patent number: 8926905
    Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.
    Type: Grant
    Filed: July 9, 2013
    Date of Patent: January 6, 2015
    Assignee: Fluidigm Corporation
    Inventors: Marc A. Unger, Geoffrey Richard Facer, Barry Clerkson, Christopher G. Cesar, Neil Switz
  • Patent number: 8926813
    Abstract: Devices and methods for detecting the length of analytes, and/or sequencing analytes are provided in which two or more electrical signals are obtained as an analyte traverses a fluidic channel. Detection of the relative position of probes hybridized to a biopolymer and/or the length of the analyte (e.g., a biopolymer) does not rely on the absolute time between detection events of a given electrical signal to determine a distance associated with the biopolymer. Instead, multiple signals are obtained as functions of time) corresponding to a plurality of detector volumes at known locations along a fluidic channel through which the biopolymer passes, and the distances are determined from the multiple signals.
    Type: Grant
    Filed: August 6, 2012
    Date of Patent: January 6, 2015
    Assignee: Nabsys, Inc.
    Inventor: John S. Oliver
  • Publication number: 20150001085
    Abstract: An apparatus for investigating a molecule comprising a channel provided in a substrate, a metallic moiety capable of plasmon resonance which is associated with the channel in a position suitable for the electromagnetic field produced by the metallic moiety to interact with a molecule passing therethrough, means to induce a molecule to pass through the channel, means to induce surface plasmon resonance in the metallic moiety; and means to detect interaction between the electromagnetic field produced by the metallic moiety and a molecule passing through the channel. Methods of investigating molecules are also provided.
    Type: Application
    Filed: September 19, 2014
    Publication date: January 1, 2015
    Inventor: Cameron FRAYLING
  • Publication number: 20140371102
    Abstract: Disclosed are embodiments of methods, apparatus, systems, compositions, and articles of manufacture relating to identifying the source of bioparticles, such as bioparticles shed by an organism. In embodiments, a method may include collecting a sample of bioparticles from the environment, selecting from that sample the bioparticles most informative for identifying their source, and gathering data from those bioparticles to form bioparticle signatures; the bioparticle signatures may be processed into a multi-dimensional vector which may be compared to a multi-dimensional vector derived from a standard using a pattern recognition strategy. In embodiments, an apparatus may include a particle collection device to collect a sample, a transfer device to select bioparticles, and a detector that restricts the movement of the bioparticles; the restricted movement may be used to produce a bioparticle signature.
    Type: Application
    Filed: September 1, 2014
    Publication date: December 18, 2014
    Inventors: Mark A. Hayes, Thomas J. Taylor
  • Publication number: 20140346047
    Abstract: A micro-particle sorting apparatus is provided including a microchip in which a flow path through which liquid containing a micro particle flows and an orifice; an oscillating element configured to transform the liquid into a liquid drop; a charge means for adding an electric charge to the discharged liquid drop; an optical detection means; paired electrodes sandwiching the moving liquid drop; and at least a container that collects the liquid drop, and wherein the flow path is configured to gradually decrease, from upstream of the orifice, in cross-section area perpendicular to the liquid-delivering direction between the location at which the optical property is detected and the location of the orifice.
    Type: Application
    Filed: July 2, 2014
    Publication date: November 27, 2014
    Inventor: Masataka SHINODA
  • Publication number: 20140339090
    Abstract: An example micro-fluidic device includes a micro-fluidic channel having an inner surface and a plurality of pillars positioned along the inner surface. The device further includes a plurality of power supplies connected to the pillars. Another example micro-fluidic device includes a micro-fluidic channel having an inner surface and a plurality of pillars positioned along the inner surface. The device further includes a power supply. The pillars are grouped into at least two groups of pillars, each group of pillars including at least two pillars, and all pillars of at least one group of pillars are connected to the power supply. In another example, a sensing system for detecting bioparticles includes a micro-fluidic device, wherein a surface of each pillar comprises functionalized plasmonic nanoparticles or functionalized SERS nanoparticles, a radiation source for radiating the micro-fluidic device, and a detector for detecting SERS signals or surface plasmon resonance.
    Type: Application
    Filed: May 17, 2014
    Publication date: November 20, 2014
    Applicant: IMEC
    Inventors: Chengjun Huang, Chengxun Liu, Liesbet Lagae, Paolo Fiorini, Benjamin Jones
  • Patent number: 8882980
    Abstract: Devices and methods for detecting an analyte are provided. Devices for voltage sensing of analytes may comprise a fluidic channel defined in a substrate, a pair of sensing electrodes disposed in a fluidic channel for sensing voltage therein, and a pair of electromotive electrodes for applying potential along the fluidic channel. The pair of sensing electrodes may include a first and second sensing electrode disposed at two discrete locations along the length of the fluidic channel and the pair of electromotive electrodes may be disposed at a first end and a second end of the fluidic channel. The fluidic channel may include a nanochannel or a microchannel.
    Type: Grant
    Filed: September 3, 2009
    Date of Patent: November 11, 2014
    Assignee: NABsys, Inc.
    Inventors: Xinsheng Ling, Barrett Bready, John S. Oliver, Maryam Jouzi, Leo Petrossian
  • Publication number: 20140318971
    Abstract: A nanosensor for detecting molecule characteristics includes a membrane having an opening configured to permit a charged carbon nanotube to pass but to block a molecule attached to the carbon nanotube. The opening is filled with an electrolytic solution. An electric field generator is configured to generate an electric field relative to the opening to drive the charged carbon nanotubes through the opening. A sensor circuit is coupled to the electric field generator to sense current changes due to charged carbon nanotubes passing into the opening, and to bias the electric field generator to determine a critical voltage related to a force of separation between the carbon nanotube and the molecule.
    Type: Application
    Filed: August 15, 2013
    Publication date: October 30, 2014
    Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Binquan Luan, Ruhong Zhou
  • Patent number: 8858770
    Abstract: Plastic electrophoresis separation chips are provided comprising a plurality of microfluidic channels and a detection window, where the detection window comprises a thin plastic; and the detection window comprises a detection region of each microfluidic channel. Such chips can be bonded to a support provided an aperture is provided in the support to allow detection of samples in the electrophoresis chip at the thin plastic detection window. Further, methods for electrophoretically separating and detecting a plurality of samples on the plastic electrophoresis separation chip are described.
    Type: Grant
    Filed: April 4, 2008
    Date of Patent: October 14, 2014
    Assignee: NetBio, Inc.
    Inventors: Eugene Tan, Cheuk Wai Kan, Heung Chuan Lam
  • Publication number: 20140262784
    Abstract: The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.
    Type: Application
    Filed: May 25, 2012
    Publication date: September 18, 2014
    Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED
    Inventors: James Clarke, James White, John Milton, Clive Brown
  • Publication number: 20140262783
    Abstract: An integrated semiconductor device for manipulating and processing bio-entity samples and methods are described. The device includes a lower substrate, at least one optical signal conduit disposed on the lower substrate, at least one cap bonding pad disposed on the lower substrate, a cap configured to form a capped area, and disposed on the at least one cap bonding pad, a microfluidic channel, wherein a first side of the microfluidic channel is formed on the lower substrate and a second side of the microfluidic channel is formed on the cap, a photosensor array coupled to sensor control circuitry, and logic circuitry coupled to the fluidic control circuitry, and the sensor control circuitry.
    Type: Application
    Filed: March 14, 2013
    Publication date: September 18, 2014
    Inventor: Taiwan Semiconductor Manufacturing Company, Ltd.
  • Patent number: 8828211
    Abstract: The invention features the use of graphene, a one atom thick planar sheet of bonded carbon atoms, in the formation of artificial lipid membranes. The invention also features the use of these membranes to detect the properties of polymers (e.g., the sequence of a nucleic acid) and identify transmembrane protein-interacting compounds.
    Type: Grant
    Filed: June 8, 2011
    Date of Patent: September 9, 2014
    Assignee: President and Fellows of Harvard College
    Inventors: Slaven Garaj, Daniel Branton
  • Patent number: 8815069
    Abstract: A disposable capillary electrophoresis detecting device includes a fixing device, a capillary electrophoresis microchip, and an electrochemical sensor microchip. The fixing device includes two chip-fixing bases having a first chip-holding cavity horizontally arranged and a second chip-holding cavity vertically arranged. The second chip-holding cavity is substantially perpendicular to the first chip-holding cavity and faces an end portion thereof. The capillary electrophoresis microchip is horizontally placed in the first chip-holding cavity. The electrochemical sensor microchip is vertically placed in the second chip-holding cavity. In the electrochemical sensor microchip, a patterned insulation layer is located on a detecting electrode, exposes a sensor area of the detecting electrode, and is extended to two sides of the sensor area.
    Type: Grant
    Filed: July 16, 2013
    Date of Patent: August 26, 2014
    Assignee: National Chung Hsing University
    Inventors: Ching-Chou Wu, Yi-Tong Pan
  • Patent number: 8771491
    Abstract: Methods and systems for sequencing a biological molecule or polymer, e.g., a nucleic acid, are provided. One or more donor labels, which are attached to a pore or nanopore, may be illuminated or otherwise excited. A polymer having a monomer labeled with one or more acceptor labels, may be translocated through the pore. Either before, after or while the labeled monomer of the polymer passes through, exits or enters the pore, energy may be transferred from the excited donor label to the acceptor label of the monomer. As a result of the energy transfer, the acceptor label emits energy, and the emitted energy is detected in order to identify the labeled monomer of the translocated polymer and to thereby sequence the polymer.
    Type: Grant
    Filed: March 21, 2012
    Date of Patent: July 8, 2014
    Assignee: Quantapore, Inc.
    Inventor: Martin Huber
  • Publication number: 20140174927
    Abstract: Provided herein are methods and devices for characterizing a biomolecule parameter by a nanopore-containing membrane, and also methods for making devices that can be used in the methods and devices provided herein. The nanopore membrane is a multilayer stack of conducting layers and dielectric layers, wherein an embedded conducting layer or conducting layer gates provides well-controlled and measurable electric fields in and around the nanopore through which the biomolecule translocates. In an aspect, the conducting layer is graphene.
    Type: Application
    Filed: July 26, 2012
    Publication date: June 26, 2014
    Inventors: Rashid Bashir, Bala Murali Venkatesan
  • Publication number: 20140166484
    Abstract: An integrated semiconductor device for manipulating and processing bio-entity samples is disclosed. The device includes a microfluidic channel that is coupled to fluidic control circuitry, a photosensor array coupled to sensor control circuitry, an optical component aligned with the photosensor array to manipulate a light signal before the light signal reaches the photosensor array, and a microfluidic grid coupled to the microfluidic channel and providing for transport of bio-entity sample droplets by electrowetting. The device further includes logic circuitry coupled to the fluidic control circuitry and the sensor control circuitry, with the fluidic control circuitry, the sensor control circuitry, and the logic circuitry being formed on a first substrate.
    Type: Application
    Filed: December 17, 2012
    Publication date: June 19, 2014
    Applicant: Taiwan Semiconductor Manufacturing Company, Ltd.
    Inventors: Yiu-Hsien Chang, Chun-Ren Cheng
  • Publication number: 20140166487
    Abstract: A solid state molecular sensor having an aperture extending through a thickness of a sensing material is configured with a continuous electrically-conducting path extending in the sensing material around the aperture. A supply reservoir is connected to provide a molecular species, having a molecular length, from the supply reservoir to an input port of the aperture. A collection reservoir is connected to collect the molecular species from an output port of the aperture after translocation of the molecular species from the supply reservoir through the sensing aperture. The sensing aperture has a length between the input and output ports, in the sensing material, that is substantially no greater than the molecular length of the molecular species from the supply reservoir. An electrical connection to the sensing material measures a change in an electrical characteristic of the sensing material during the molecular species translocation through the aperture.
    Type: Application
    Filed: February 21, 2014
    Publication date: June 19, 2014
    Applicant: President and Fellows of Harvard College
    Inventors: Charles M. Lieber, Qihua Xiong, Ping Xie, Ying Fang
  • Publication number: 20140158537
    Abstract: Microfluidic methods of assaying molecule switching are provided. Aspects of the methods include microfluidically separating a sample containing the molecule of interest and then employing the resultant separation pattern to determine a switching characteristic of the molecule. Also provided are microfluidic devices, as well as systems and kits that include the devices, which find use in practicing embodiments of the methods. The methods, devices, systems and kits find use in a variety of different applications, such as analytical and diagnostic assays.
    Type: Application
    Filed: September 24, 2013
    Publication date: June 12, 2014
    Inventors: Amy E. Herr, Augusto M. Tentori, Alex J. Hughes
  • Patent number: 8728292
    Abstract: An apparatus (1) for the measurement of a concentration of a charged species in a sample (10) is disclosed. The sample (10) comprises a plurality of types of charged species and at least one insoluble component. The apparatus (1) comprises a first circuit with a voltage control device (54) connectable to two first electrodes (30, 30?) arranged along a channel (12) holding the sample (10) and a second circuit with a conductivity detection device (55) connectable to two second electrodes (5, 5?) arranged in the channel (12). The first circuit and the second circuit are dc and ac electrically isolated from each other.
    Type: Grant
    Filed: February 17, 2009
    Date of Patent: May 20, 2014
    Assignee: Medimate Holding B.V.
    Inventors: Steven S. Staal, Jan Floris, Stefan Otto Lenk
  • Patent number: 8721859
    Abstract: An analysis apparatus is an apparatus that performs electrophoresis using a microchip provided with a channel. The analysis apparatus includes a cooling unit (an electron cooling element and a driving circuit) that cools the microchip, a voltage application unit (electrodes and a power supply circuit) that applies voltage to a buffer solution filled in the channel of the microchip, an optical analysis unit (a light source, a light receiving element, and an analysis unit) that conducts, through the microchip, optical analysis of a sample introduced in the channel, and a control unit that controls the cooling unit, the voltage application unit, and the optical analysis unit. The control unit causes the cooling unit to start cooling the microchip, and after the microchip has been cooled, causes the voltage application unit and the optical analysis unit to operate.
    Type: Grant
    Filed: March 22, 2012
    Date of Patent: May 13, 2014
    Assignee: ARKRAY, Inc.
    Inventors: Yasunori Shiraki, Daisuke Matsumoto, Yusuke Nakayama, Genki Adachi
  • Patent number: 8721968
    Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.
    Type: Grant
    Filed: September 19, 2013
    Date of Patent: May 13, 2014
    Assignee: Fluidigm Corporation
    Inventors: Marc A. Unger, Geoffrey Richard Facer, Barry Clerkson, Christopher G. Cesar, Neil Switz
  • Publication number: 20140110259
    Abstract: A biopolymer optical analysis apparatus is provided with a biopolymer characteristic analysis chip including a solid substrate, nanopores provided in the substrate, and electrically conductive thin films on the substrate, at least portions of the thin films facing the nanopores, a light source and an irradiation optical system for producing Raman scattered lights from biopolymers entering the nanopores, and a detection device including a Raman scattered light collecting system, a separating component that splits collected light into transmitted light and reflected light, an image-forming optical system through which the split lights form images, and a two-dimensional detector for detecting the lights forming the images. External light from the light source and the irradiation optical system is applied to the characteristic analysis chip, and the detection device detects Raman scattered lights from biopolymers in the analysis chip.
    Type: Application
    Filed: May 29, 2012
    Publication date: April 24, 2014
    Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION
    Inventors: Satoshi Takahashi, Nobutaka Kumazaki