Capillary Electrophoresis Type Patents (Class 204/601)
  • Patent number: 10301666
    Abstract: Contemplated methods and devices are drawn to preparation and analysis of analytes from biological samples. In a preferred embodiment the analytes are nucleic acids that are both released from biological compartments present in the sample and fragmented through the use of a voltage potential applied to a pair of electrodes. The nucleic acids thus prepared are subsequently characterized.
    Type: Grant
    Filed: January 17, 2017
    Date of Patent: May 28, 2019
    Assignee: General Atomics
    Inventors: Shana O. Kelley, Susan Bortolin, Reginald James McKenzie Orton, Stefan Christopher Wiechula
  • Patent number: 10285316
    Abstract: The movement time of a board is shortened in an electronic component mounting device that performs heating and component mounting. Heating and component mounting are performed as a board is moved in the direction of arrow A; the board is moved by an in-conveyor device in the heating region, and by a shuttle device on rails in the component mounting region. When comparing chains with rails, the friction generated between the board is smaller with chains, thus the movement speed of the in-conveyor is faster. This means, compared to a case in which the board is moved by the rails and shuttle device in both the heating region and the component mounting region, the movement time of the board is shorter. Also, because there are no chains below the rails, the movable range of a flow tank is larger, and the component mountable region is larger.
    Type: Grant
    Filed: October 21, 2013
    Date of Patent: May 7, 2019
    Assignee: FUJI CORPORATION
    Inventors: Katsunori Tanaka, Tsuyoshi Hamane, Kazuya Degura
  • Patent number: 10180676
    Abstract: To provide a microchip-type optical measuring apparatus which is able to automatically perform position adjustment of a microchip with respect to an optical axis of laser with high accuracy. A microchip-type optical measuring apparatus includes an irradiation detection unit which detects light generated by irradiating a microchip with laser, a position adjustment unit which changes a relative position of the microchip with respect to the irradiation detection unit, and a control unit which outputs a movement signal for a position in which an integrated value or an average value of a detected intensity of the light in a preset region becomes high to the position adjustment unit.
    Type: Grant
    Filed: January 19, 2018
    Date of Patent: January 15, 2019
    Assignee: Sony Corporation
    Inventors: Yosuke Muraki, Fumitaka Otsuka
  • Patent number: 10126294
    Abstract: In one embodiment, the invention is to a sample metering device, comprising a sample holding chamber oriented between a sample entry port and a sample isolation unit and having a diluent introduction port disposed therebetween for introduction of a diluent into the sample holding chamber. The volume within the sample holding chamber between the diluent introduction port and the sample isolation unit defines a metered volume of a sample for analysis. In another embodiment, the invention is to an apparatus and method for rapid determination of analytes in liquid samples by various assays including immunoassays incorporating a sample dilution feature, preferably suitable for low range sample dilution, and preferably capable of being used in the point-of-care diagnostic field.
    Type: Grant
    Filed: May 14, 2015
    Date of Patent: November 13, 2018
    Assignee: Abbott Point of Car Inc.
    Inventor: Cary James Miller
  • Patent number: 10113947
    Abstract: According to one embodiment, a semiconductor analysis chip for detecting a particle in a sample liquid includes a semiconductor substrate, a first flow channel provided in a surface portion of the semiconductor substrate, a second flow channel provided in a surface portion of the semiconductor substrate, part of the second flow channel contacting or intersecting the first flow channel, a micropore provided in a contact portion or an intersection of the first and second flow channels, and configured to permit the particle to pass therethrough, a first electrode provided in the first flow channels, a second electrode provided in the second passage, and a third electrode provided in the first flow channel downstream of the first electrode.
    Type: Grant
    Filed: March 2, 2016
    Date of Patent: October 30, 2018
    Assignee: KABUSHIKI KAISHA TOSHIBA
    Inventors: Kentaro Kobayashi, Hiroshi Hamasaki, Naofumi Nakamura
  • Patent number: 10058818
    Abstract: Present embodiments provide an energy-efficient separator having at least two chambers fluidically isolated by a gas permeable membrane for the removal of dissolved gases from a sample which, in some aspects, enables cost-effective monitoring of liquid samples to be performed remotely and automatically, and in other aspects is suited for various other applications where removal and/or regulation of gases in a sample are desirable.
    Type: Grant
    Filed: May 27, 2015
    Date of Patent: August 28, 2018
    Assignee: UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION, INC.
    Inventors: Mohamed M. Marei, Richard P. Baldwin, Thomas J. Roussel, Jr., Robert S. Keynton
  • Patent number: 9968930
    Abstract: A microfluidic product utilizing gradient surface energy coatings for fluid control comprising a plurality of fluid passages wherein at least one fluid passage comprises a coating configured to control liquid flow wherein the coating configured to control liquid flow comprises a gradient surface energy coating from a proximal location to a distal location on a surface of the fluid passage. The product can include uniform regions and surface gradient regions in the same passage. Coating compositions and product dimensions can be selected to provide control over different flow properties including fluid velocity, reduction and acceleration of fluid flow, and starting and stopping fluid flow.
    Type: Grant
    Filed: March 26, 2014
    Date of Patent: May 15, 2018
    Inventor: Brian David Babcock
  • Patent number: 9927396
    Abstract: Aspects of the innovations presented herein relate to improved systems that in some embodiments perform capillary electrophoresis (CE) and CE in conjunction with electrospray ionization (ESI) as an input to a mass spectrometry system (MS). Some embodiments use a high voltage isolated CE power supply that is configured to float on the high voltage output of an ESI-MS power supply, with a protective resistance in the ESI-MS path, as well as DC/DC converter isolation and communication system isolation for the isolated CE power supply. Some embodiments additionally use a cartridge assembly integrating separation and conductive fluid capillaries with fluid cooling and protective retractable housings for the capillary end portions and for the ESI output. The protective housing may further be used with an adapter for interfacing with different MS systems.
    Type: Grant
    Filed: October 26, 2012
    Date of Patent: March 27, 2018
    Assignee: DH Technologies Development Pte. Ltd.
    Inventors: Brian D. Peterson, Dale Alan Owens, Peter S. Kotowski, Sunil S. Deliwala, Craig A. Hudson, James H. Reyes, Clive Cheung, Tom Tran, Anna X. Lou, Jane Luo, Jean-Marc Rene Lucien Busnel, Chitra K. Ratnayake
  • Patent number: 9927399
    Abstract: A method for analyzing a protein and a peptide, includes: providing a capillary for isoelectric focusing; providing a capillary device for separation and analysis having the capillary and a solid-phase extraction column being unified as a single tube-like structure; providing an electrophoresis instrument having the capillary device and the mechanism regulating the pressure difference at both ends of the capillary device; introducing a sample containing a target protein or peptide into the solid-phase extraction column to let the target protein or peptide be adsorbed on the column, and filling the capillary device with a carrier ampholyte solution; starting separation by isoelectric focusing after eluting the target protein or peptide by filling the solid-phase extraction column with electrode solution or acid or base solution, or after firstly eluting the target protein or peptide with an eluting solution containing carrier ampholyte and secondly filling the solid-phase extraction column with electrode solut
    Type: Grant
    Filed: October 14, 2014
    Date of Patent: March 27, 2018
    Assignees: NICHIEI INDUSTRY CO., LTD.
    Inventors: Kiyohito Shimura, Toshihiko Nagai, Shuuichi Fukuhara, Yoshi-ichi Seto
  • Patent number: 9915935
    Abstract: To provide a microchip-type optical measuring apparatus which is able to automatically perform position adjustment of a microchip with respect to an optical axis of laser with high accuracy. A microchip-type optical measuring apparatus includes an irradiation detection unit which detects light generated by irradiating a microchip with laser, a position adjustment unit which changes a relative position of the microchip with respect to the irradiation detection unit, and a control unit which outputs a movement signal for a position in which an integrated value or an average value of a detected intensity of the light in a preset region becomes high to the position adjustment unit.
    Type: Grant
    Filed: January 28, 2013
    Date of Patent: March 13, 2018
    Assignee: Sony Corporation
    Inventors: Yosuke Muraki, Fumitaka Otsuka
  • Patent number: 9863904
    Abstract: A method of analyzing a molecule is disclosed. A voltage source is selectively connected to or disconnected from a capacitor using a switch controlled by a reset signal. A charge is stored in a capacitor when the voltage source is connected to the capacitor. The capacitor is discharged through a nanopore in a membrane when the voltage source is disconnected from the capacitor. A duty cycle of the reset signal is determined such that the voltage source and the capacitor is connected for at least a one tenth portion of a reset signal period and disconnected for a remaining portion of the reset signal period, such that a voltage across the nanopore is maintained at a higher level during the portion of the reset signal period in which the connection is maintained than during the remaining portion of the reset signal period in which the connection is not maintained.
    Type: Grant
    Filed: December 16, 2015
    Date of Patent: January 9, 2018
    Assignee: Genia Technologies, Inc.
    Inventors: Roger J. A. Chen, Hui Tian, Bill Maney
  • Patent number: 9683994
    Abstract: The present invention provides novel methods for performing pulsed field mobility shift assays in microfluidic devices. In particular, the methods of the invention utilize differences between electrophoretic mobilities (e.g., as between reactants and products, especially in non-fluorogenic reactions) in order to separate the species and thus analyze the reaction.
    Type: Grant
    Filed: July 9, 2012
    Date of Patent: June 20, 2017
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Andrea W. Chow, John C. Owicki, J. Wallace Parce
  • Patent number: 9651527
    Abstract: An interface for a mass spectrometer is disclosed comprising a microfluidic substrate, tile or cartridge 1 comprising a liquid chromatography separation column and an electrospray emitter 2. A counter electrode 4 is arranged downstream of a tip of the electrospray emitter 2 and is arranged and adapted to direct ions towards an atmospheric pressure interface or ion inlet aperture 5 of a mass spectrometer.
    Type: Grant
    Filed: December 2, 2015
    Date of Patent: May 16, 2017
    Assignee: Micromass UK Limited
    Inventors: David S. Douce, John Chipperfield, Stevan Bajic, James Murphy, David Gordon
  • Patent number: 9595430
    Abstract: A self-assembled engineered lattice of nanometer-scale silica particles, or other suitable particles generally resembling regularly-sized spheres, is configured in a separation bed for electrophoresis, isoelectric focusing, chromatography, or other voltage-induced separation of analytes. After separation, the analytes are immobilized on the separation bed and then ionized using matrix-assisted laser desorption/ionization (MALDI) for use with a mass spectrometer. The nanoparticles can be coated with polymers that activate to immobilize the analytes or assist with MALDI. The separation can occur in two dimensions.
    Type: Grant
    Filed: March 25, 2015
    Date of Patent: March 14, 2017
    Assignees: LI-COR, INC., Purdue Research Foundation
    Inventors: Michael D. Furtaw, Jon P. Anderson, Mary J. Wirth, Robert E. Birdsall, Yimin Hua, Zhaorui Zhang
  • Patent number: 9580679
    Abstract: Disclosed herein are methods and systems for use in preparing a sample. The methods and systems may be used for lysing one or more structures in a sample (e.g., cells, viral particles, etc.). The methods and compositions may comprise a microfluidic chip or use thereof. The microfluidic chips disclosed herein may comprise (a) a substrate comprising a chamber, wherein at least one mechanical element may be located within the chamber; (b) a thermal element in contact with the chamber; and (c) at least one aperture within the surface of the substrate, wherein the aperture may be configured to insulate the chamber.
    Type: Grant
    Filed: September 20, 2013
    Date of Patent: February 28, 2017
    Assignee: California Institute of Technology
    Inventors: Samuel Njoroge, John Gorman, George Maltezos, Axel Scherer
  • Patent number: 9566504
    Abstract: The present invention relates to an air cleaner for removing air pollutants from an air stream, for instance such as the cleaning exhaust/intake gas of an amusement machine, said air cleaner comprising a filter device including at least one filter layer (4a, 4b) held by a filter holder (5) in said airflow substantially perpendicular to a main flow direction thereof and including a plurality of preferably stick-shaped filter elements (6) neighbouring each other. In accordance with the present invention, the filter device has no closed surrounding along the circumference of the filter layers, but provides for an open side along at least a portion of the periphery of the filter layer. At least one circumferential side section (7, 8) of the filter layer parallel to or tangential to the longitudinal axis of an outermost filter element is formed as an open side free of flow control elements surrounding the filter layer.
    Type: Grant
    Filed: February 26, 2014
    Date of Patent: February 14, 2017
    Assignees: NOVOMATIC AG, DEXWET USA LLC
    Inventors: Christoph Bichl, Heinz Winkler, Alois Homer, Erich Petein, Maximillian Vojta
  • Patent number: 9539574
    Abstract: This invention provides methods and systems for injection of analytes into a separation channel for resolution and detection. Samples can be preconditioned and concentrated by isotachophoresis (ITP) before the injection is triggered by a detected voltage event. Separation of analytes from other sample constituents can be enhanced using skewing channel ITP.
    Type: Grant
    Filed: October 11, 2010
    Date of Patent: January 10, 2017
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Charles Park, Persefoni Kechagia, Michael Spaid, Morten Jensen, Irina G. Kazakova, Josh Molho
  • Patent number: 9535033
    Abstract: The analysis method allows analysis of samples with high sensitivity, irrespective of interelectrode distance. The method includes: a step of applying a voltage between a first electrode pair such that an electric field is formed in a direction intersecting a migration direction of a sample; a step of placing a solution, including an electrochemically active molecule that produces a redox reaction at the electrode pair, between the first electrode pair; a step of causing the sample to migrate; and a step of measuring an amount of change in current flow between the first electrode pair.
    Type: Grant
    Filed: August 2, 2013
    Date of Patent: January 3, 2017
    Assignee: QUANTUM BIOSYSTEMS INC.
    Inventors: Tomoji Kawai, Takahito Ohshiro, Masateru Taniguchi
  • Patent number: 9494553
    Abstract: An electrophoresis chip that can be small and simple and that can analyze a sample with high accuracy is provided. The electrophoresis chip includes an upper substrate 4, a lower substrate 1, an introduction reservoir 2a, a recovery reservoir 2b and a capillary channel for sample analysis 3x. The introduction reservoir 2a and the recovery reservoir 2b are formed in the lower substrate 1. The introduction reservoir 2a and the recovery reservoir 2b are in communication with each other via the capillary channel for sample analysis 3x. The introduction reservoir 2a receives a sample to be measured. The sample is electrophoretically introduced directly into the capillary channel for sample analysis 3x by creating a potential difference between the introduction reservoir 2a and the recovery reservoir 2b, and is also analyzed in the capillary channel for sample analysis 3x during the separation of the sample while the sample is being continuously supplied.
    Type: Grant
    Filed: April 28, 2008
    Date of Patent: November 15, 2016
    Assignees: National Institute of Advanced Industrial Science and Technology, ARKRAY, Inc.
    Inventors: Yoshihide Tanaka, Yusuke Nakayama, Satoshi Yonehara
  • Patent number: 9470724
    Abstract: A method and a system are shown for attachment of leads for the electrical sensing of the voltage on conductive structures; connections attached per this invention have the valuable property of very low thermoelectric errors (due to Seebeck effect), among several other beneficial properties. The described method can be applied in the factory setting as well as in the field. This system is especially suitable for applications with high-precision resistive shunts utilized in the measurements of the electric current.
    Type: Grant
    Filed: August 12, 2015
    Date of Patent: October 18, 2016
    Assignee: Sendyne Corporation
    Inventor: Victor Marten
  • Patent number: 9360403
    Abstract: The present invention provides a device and methods of use thereof in concentrating a species of interest and/or controlling liquid flow in a device. The methods make use of a device comprising a fluidic chip comprising a planar array of channels through which a liquid comprising a species of interest can be made to pass with at least one rigid substrate connected thereto such that at least a portion of a surface of the substrate bounds the channels, and a high aspect ratio ion-selective membrane is embedded within the chip, attached to at least a portion of the channels. The device comprises a unit to induce an electric field in the channel and a unit to induce an electrokinetic or pressure driven flow in the channel.
    Type: Grant
    Filed: April 12, 2013
    Date of Patent: June 7, 2016
    Assignee: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Jongyoon Han, Sung Jae Kim
  • Patent number: 9330612
    Abstract: A device cover includes a protective layer and a display layer. The display layer includes a plurality of display pixels. Each display pixel includes a first fluid having a first color characteristic and a second fluid having a second color characteristic.
    Type: Grant
    Filed: July 5, 2014
    Date of Patent: May 3, 2016
    Inventor: Robert Reid
  • Patent number: 9193748
    Abstract: The present invention concerns the use of methods and compositions for the isolation of small RNA molecules (100 nucleotides or fewer), such as microRNA and siRNA molecules. Such molecules are routinely lost in commonly used isolation procedures and therefore the present invention allows for a much higher level of enrichment or isolation of these small RNA molecules.
    Type: Grant
    Filed: February 27, 2013
    Date of Patent: November 24, 2015
    Assignee: Applied Biosystems, LLC
    Inventor: Richard Conrad
  • Patent number: 9168527
    Abstract: An electrokinetic fluidic system (100, 100?, 100?) for controlling liquid flow in e.g. a lab-on-a-chip system (200) comprising a first and a second electrode (10, 10?) said first and second electrode comprising a polymer based or oxide based conductive, electrochemically active electrode material, said electrode material being adapted to be subjected to an electrochemical reaction when in use in said electrokinetic fluid system (100).
    Type: Grant
    Filed: February 22, 2011
    Date of Patent: October 27, 2015
    Assignee: LunaMicro AB
    Inventors: Nathaniel D. Robinson, Per Erlandsson
  • Patent number: 9110044
    Abstract: An embodiment of the present invention relates to a system for the integrated and automated analysis of DNA or protein, including a single-use cartridge, an analysis device comprising a control device, and devices for capturing and processing signals. An embodiment of the present invention relates, in particular, to the control device for carrying out a completely automatic process and evaluation of molecular diagnostic analysis via single-use cartridges (Lab-on-a-Chip). The first devices are provided for controlling an analysis process which occurs in the cartridge, subsequently the displacement and the thermostatisation of liquids, and the second devices are provided for processing the signals which are obtained during the analysis. The first and the second devices are synchronized in such a manner that the analysis process of the sample can be carried out in a totally integrated manner thus producing an immediate result.
    Type: Grant
    Filed: May 22, 2006
    Date of Patent: August 18, 2015
    Assignee: Boehringer Ingelheim Vetmedica GmbH
    Inventors: Walter Gumbrecht, Peter Paulicka
  • Patent number: 9052309
    Abstract: The invention is to devices and methods for rapid determination of analytes in liquid samples. The devices and methods incorporate a sample dilution feature and multiple immunosensors for performing a ratiometric immunoassay on a first analyte and a second analyte, for example, hemoglobin and hemoglobin A1c or albumin and glycosylated albumin. The devices are preferably capable of being used in the point-of-care diagnostic field.
    Type: Grant
    Filed: December 1, 2011
    Date of Patent: June 9, 2015
    Assignee: Abbott Point of Care Inc.
    Inventor: Cary James Miller
  • Publication number: 20150144489
    Abstract: A disposable cartridge used in a digital microfluidics system has a bottom layer with first hydrophobic surface, a rigid cover plate with second hydrophobic surface, and a gap there-between. The bottom layer is a flexible film on an uppermost surface of a cartridge accommodation site of a system, attracted to and spread over the uppermost surface by an underpressure. A lower surface of the plate and the flexible bottom layer are sealed to each other. The assembled cartridge is removed from the cartridge accommodation site in one piece and potentially includes samples and processing fluids. The system has a base unit and a cartridge accommodation site with an electrode array of individual electrodes and a central control unit for controlling selection of individual electrodes and for providing these electrodes with individual voltage pulses for manipulating liquid droplets within the gap by electrowetting.
    Type: Application
    Filed: January 6, 2014
    Publication date: May 28, 2015
    Applicant: TECAN TRADING AG
    Inventors: Daniel Hoffmeyer, Tiffany Lay, Travis Lee, Marc N. Feiglin, Werner Halg, Anne R. Kopf-Sill
  • Publication number: 20150136602
    Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.
    Type: Application
    Filed: November 24, 2014
    Publication date: May 21, 2015
    Inventors: Stevan B. Jovanovich, William D. Nielsen, David S. Cohen, Michael Recknor, Mattias Vangbo, Ezra Van Gelder, Lars Majlof, Omar El-Sissi
  • Patent number: 9034163
    Abstract: An electrode assembly for capillary electrophoresis (CE) comprises a manifold (310), a connector (305) a sheath (300), and a seal (325). A capillary tube (100) passes through the manifold, the connector, the sheath, and the seal, stopping just beyond the end of the sheath. The sheath is fillable with water (330) or another fluid that cools the capillary tube in the vicinity of the electrode, thereby preventing degradation of a sample due to heat. The sheath may be metal or plastic with a metal sleeve electrode on its exterior. The sheath is sufficiently strong to penetrate a rubber or other pierceable cap on a vial. The manifold and connector incorporate an air path (605, 312, 307) so that when the electrode is fully inserted into a vial, the contents (650) of the vial are at atmospheric pressure (or another applied pressure or vacuum).
    Type: Grant
    Filed: November 20, 2012
    Date of Patent: May 19, 2015
    Inventor: Tiansong Wang
  • Patent number: 9034161
    Abstract: The present invention provides methods, devices, and kits for separating and selecting top sperm from a sperm sample of a subject. In one aspect, for example, such a method can include removing a portion of negatively charged protein from sperm in the sperm sample, immobilizing the sperm, electrophoretically separating the sperm, and selecting mature sperm based on electromotility properties.
    Type: Grant
    Filed: August 23, 2013
    Date of Patent: May 19, 2015
    Inventors: Douglas T. Carrell, Luke Simon
  • Publication number: 20150129427
    Abstract: The present invention relates to a process for the extraction of analyte compounds from a sample comprising one or more analytes in a donor phase into an acceptor phase, comprising the steps of: a) providing an electrically conductive donor phase comprising the compounds in a first electrically conductive solvent or solvent blend, and an electrode arranged in electrically conductive contact with the donor phase, b) providing an electrically conductive acceptor phase in electrically conductive contact with a second electrode; and c) providing an insulator phase in fluid communication with at least one of the donor phase and the acceptor phase, wherein the insulator phase is immiscible with the donor phase and/or the acceptor phase, and d) (d) applying an electrical field between the first and the second electrode.
    Type: Application
    Filed: April 19, 2013
    Publication date: May 14, 2015
    Inventors: Robert-Jan Raterink, Peter Lindenburg, Thomas Hankemeier
  • Publication number: 20150122655
    Abstract: A sample separation apparatus (200) for separating a fluidic sample, the sample separation apparatus (200) comprising a first separation unit (204) for separating the fluidic sample, a first fluid drive (202) configured for conducting the fluidic sample to be separated through the first separation unit (204), a second separation unit (208), arranged downstream of the first separation unit (204), for further separating the fluidic sample after treatment by the first separation unit (204), a second fluid drive (206) configured for at least partially conducting the fluidic sample, after treatment by the first separation unit (204), through the second separation unit (208), and a fluidic valve (218) having fluidic interfaces (222, 224, 226, 228) fluidically coupled to the first fluid drive (202) and the second fluid drive (206) and being switchable for performing the separation of the fluidic sample, wherein the sample separation apparatus (200) is configured for adjusting a pressure at a predefined position to a
    Type: Application
    Filed: May 10, 2012
    Publication date: May 7, 2015
    Inventors: Konstantin Choikhet, Klaus Witt
  • Publication number: 20150122651
    Abstract: A method of generating droplets of a dispersed phase fluid in a continuous phase fluid includes flowing the dispersed phase fluid and the continuous phase fluid to a channel junction of at least one dispersed phase channel and at least one continuous phase channel, applying at least one alternating voltage to at least two electrodes so that an alternating electric field is created at the channel junction, and generating the droplets of the dispersed phase fluid in the continuous phase fluid flowing in an output channel of the channel junction, wherein the dispersed phase fluid and the continuous phase fluid are electrically insulated from the at least two electrodes. Furthermore, a microfluidic device is described, which is configured for generating droplets of a dispersed phase fluid in a continuous phase fluid.
    Type: Application
    Filed: March 30, 2012
    Publication date: May 7, 2015
    Inventors: Jean-Christophe Baret, Say Hwa Tan, Benoit Semin
  • Publication number: 20150107998
    Abstract: Embodiments of the present disclosure digital microfluidic arrays that may be fabricated by a printing method, whereby digital microfluidic electrodes arrays are printed, via a printing method such as inkjet printing, onto a suitable substrate. In some embodiments, a substrate and/or ink is prepared or modified to support the printing of electrode arrays, such as via changes to the surface energy. In some embodiments, porous and/or fibrous substrates are prepared by the addition of a barrier layer, or, for example, by the addition or infiltration of a suitable material to render the surface capable of supporting printed electrodes. Various example embodiments involving hybrid devices formed by the printing of digital microfluidic arrays onto a substrate having a hydrophilic layer are disclosed.
    Type: Application
    Filed: February 27, 2014
    Publication date: April 23, 2015
    Applicant: THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO
    Inventors: Ryan FOBEL, Andrea KIRBY, Aaron WHEELER
  • Publication number: 20150107996
    Abstract: A method of identifying a molecule is disclosed. A molecule is drawn to a nanopore by applying a first voltage signal to a pair of electrodes during a first period, wherein the first voltage signal causes a first ionic current through the nanopore that is indicative of a property of a portion of the molecule proximate to the nanopore. The molecule is released from the nanopore by applying a second voltage signal to the pair of electrodes during a second period, wherein the second voltage signal causes a second ionic current through the nanopore. The first period and the second period are determined based at least in part on a net ionic current through the nanopore comprising the first ionic current and the second ionic current.
    Type: Application
    Filed: October 17, 2013
    Publication date: April 23, 2015
    Inventor: Roger J.A. Chen
  • Publication number: 20150101931
    Abstract: The invention features the use of graphene, a one atom thick planar sheet of bonded carbon atoms, in the formation of artificial lipid membranes. The invention also features the use of these membranes to detect the properties of polymers (e.g., the sequence of a nucleic acid) and identify transmembrane protein-interacting compounds.
    Type: Application
    Filed: September 5, 2014
    Publication date: April 16, 2015
    Inventors: Slaven GARAJ, Daniel BRANTON
  • Publication number: 20150101933
    Abstract: By driving molecules electrophoretically through a nanopore, single molecule detection can be achieved. To enhance translocational control, functionalized and non-functionalized electrodes are strategically placed around or above a nanopore. Changes in transmission spectra and input voltage detected by electrodes allow accurate identification of single molecules as they pass through a nanopore.
    Type: Application
    Filed: December 18, 2014
    Publication date: April 16, 2015
    Inventors: Andres JARAMILLO-BOTERO, William A. GODDARD, III
  • Publication number: 20150090591
    Abstract: An apparatus includes a body portion that defines a reservoir and a set of substantially flexible capillaries. The set of substantially flexible capillaries are fixedly coupled to the body portion and in fluid communication with the reservoir. A connector is configured to be coupled to the body portion to be in fluid communication with the reservoir and the set of substantially flexible capillaries. The connector is further configured to be coupled to a vacuum source. The apparatus is arranged such that at least a part of the body portion is electrically conductive. Methods for separating and detecting an analyte from a biological sample with the apparatus are also provided. For example, methods for separating and detecting one or more proteins from a cellular lysate or a purified protein are also provided.
    Type: Application
    Filed: September 27, 2013
    Publication date: April 2, 2015
    Applicant: ProteinSimple
    Inventors: Tom W. Yang, David John Roach
  • Publication number: 20150075988
    Abstract: An integrated fluidic circuit has a supporting surface that carries a first fluid to be moved at a first functional region; a dielectric structure, defining the supporting surface; and an electrode structure, coupled to the dielectric structure for generating an electric field at the first functional region, such as to modify electrowetting properties of the interface between the first fluid and the supporting surface. The dielectric structure has a first spatially variable dielectric profile at the first functional region, thus determining a corresponding spatially variable profile of the electric field, and, consequently, of the electrowetting properties of the interface between the first fluid and the supporting surface. The integrated fluidic circuit may achieve mixing between the first fluid and a second fluid.
    Type: Application
    Filed: September 17, 2014
    Publication date: March 19, 2015
    Inventor: Alessandro Paolo Bramanti
  • Publication number: 20150075990
    Abstract: A membrane structure is provided. The membrane structure may include: a membrane; at least one hole extending into the membrane configured to receive a fluid. The membrane may include a plurality of electrodes arranged to provide one or more electric fields to control a movement of the fluid within the at least one hole.
    Type: Application
    Filed: September 13, 2013
    Publication date: March 19, 2015
    Applicant: Infineon Technologies AG
    Inventors: Bernhard Fehr, Hanno Melzner, Karl Heinz Hitzlberger
  • Publication number: 20150075987
    Abstract: A device and related method for separating nanometer particles is disclosed and described. The device can include a microfluidic system including a sample input port, a fluid flow channel, and a sample output port, in which the fluid flow channel is defined by a pair of electrode walls and an insulator. A voltage device is electrically coupled to the electrode walls. The voltage device is comprised of a diode or a resistor configured to provide an electrical field within the fluid flow channel suitable for separation of nanoparticles from one another by causing a net effect of moving particles toward one of the electrode walls.
    Type: Application
    Filed: July 8, 2014
    Publication date: March 19, 2015
    Inventors: Tonguc Onur Tasci, Bruce K. Gale, William P. Johnson
  • Publication number: 20150068901
    Abstract: This disclosure provides, among other things, a nanofluidic device sensing device is provided. In certain embodiments, the device contains: a) a channel comprising a floor and a ceiling, b) an array of charge sensors in the floor and/or ceiling of the channel, arranged along the longitudinal axis of the channel; c) a capture area in the floor and/or ceiling of the channel at the entrance end of the channel; and d) a first electrode and a second electrode, wherein the first and second electrodes are positioned to provide an electrophoretic force along the longitudinal axis of the channel. Other embodiments, e.g., methods, are also described.
    Type: Application
    Filed: September 10, 2014
    Publication date: March 12, 2015
    Inventors: John Mannion, Bo Curry, Brian Jon Peter
  • Publication number: 20150072868
    Abstract: A device includes at least one nanoscale capillary and means for applying an electric voltage, said means being adapted to create an electric field at least in said capillary when said electric voltage is applied, so that, when said electric voltage is applied, a charged molecule or particle placed within the created electric field can be electrically controlled. A fluidic network structure includes the at least one nanoscale capillary. A method of using and manufacturing the fluidic network structure is also described.
    Type: Application
    Filed: April 16, 2013
    Publication date: March 12, 2015
    Applicant: QUNANO AB
    Inventors: Jonas Ohlsson, Mikael Bjork
  • Patent number: 8974650
    Abstract: A method for forming a microfluidic channel with improved flow characteristics for one or more analytes is disclosed. A microfluidic channel having modified surfaces is formed in a glass layer or glass substrate. The glass surfaces of the microfluidic channel are modified by the addition of a layer of borophosphosilicate glass. The addition of the borophosphosilicate glass results in an improved flow velocity profile of the analyte. As a result, control over the position and movement of analytes within the solution is improved.
    Type: Grant
    Filed: May 28, 2013
    Date of Patent: March 10, 2015
    Assignee: Octrolix BV
    Inventor: Rene Gerrit Heideman
  • Publication number: 20150060283
    Abstract: A technique is provided for forming a nanodevice for sequencing. A bottom metal contact is disposed at a location in an insulator that is on a substrate. A nonconducting material is disposed on top of the bottom metal contact and the insulator. A carbon nanotube is disposed on top of the nonconducting material. Top metal contacts are disposed on top of the carbon nanotube at the location of the bottom metal contact, where the top metal contacts are formed at opposing ends of the carbon nanotube at the location. The carbon nanotube is suspended over the bottom metal contact at the location, by etching away the nonconducting material under the carbon nanotube to expose the bottom metal contact as a bottom of a trench, while leaving the nonconducting material immediately under the top metal contacts as walls of the trench.
    Type: Application
    Filed: September 26, 2013
    Publication date: March 5, 2015
    Applicant: International Business Machines Corporation
    Inventors: Ali Afzali-Ardakani, Aaron D. Franklin, George S. Tulevski
  • Patent number: 8968873
    Abstract: A microfluidic device to produce polymersomes having three coaxial passageways of increasing size with fluid flowing in one direction. The first and smallest passageway contains the content of the polymersome, the middle passageway contains a block copolymer, and the largest and outer passageway contains an aqueous medium or water. The device can produce polymersomes with control of size and membrane thickness. The device will allow quantitative loading of the polymersomes in high quantities. The device is robust and easily assembled and has the ability to independently control the three streams involved in making the polymersomes.
    Type: Grant
    Filed: August 12, 2011
    Date of Patent: March 3, 2015
    Assignees: University of Connecticut, Princeton University
    Inventors: Douglas H. Adamson, Michael Stredney, Robert K. Prud'homme, Mustafa Erhan Yildiz
  • Patent number: 8969090
    Abstract: In some embodiments, an analyte detection system is provided that includes a nanochannel, an electrode arrangement, and a plurality of nanoFET devices disposed in the nanochannel. A plurality of nucleic acid base detection components can be used that include a plurality of nanopores, a plurality of nanochannels, a plurality of hybridization probes, combinations thereof, and the like. According to other embodiments of the present teachings, different coded molecules are hybridized to a target DNA molecule and used to detect the presence of various sequences along the target molecule. A kit including mixtures of coded molecules is also provided. In some embodiments, devices including nanochannels, nanopores, and the like, are used for manipulating movement of DNA molecules, for example, in preparation for a DNA sequencing detection. Nanopore structures and methods of making the same are also provided as are methods of nucleic acid sequencing using the nanopore structures.
    Type: Grant
    Filed: January 4, 2011
    Date of Patent: March 3, 2015
    Assignee: Life Technologies Corporation
    Inventors: Hongye Sun, Eric S. Nordman, Mark F. Oldham, John R. O'Neill, Charles Connell, Umberto Ulmanella, Aldrich N. K. Lau, Theofilos Kotseroglou, Kenneth J. Livak
  • Patent number: 8968539
    Abstract: Disclosed here are methods useful for incorporating protein into lipid bilayers using voltage induced insertion. The methods presented herein can decrease time and costs associated with incorporation of proteins into naturally derived or artificially created lipid bilayers. A method for incorporating a protein capable of translocating a ligand also is disclosed herein.
    Type: Grant
    Filed: March 7, 2012
    Date of Patent: March 3, 2015
    Assignee: Electronic Biosciences, Inc.
    Inventors: Ryan Dunnam, Geoffrey Barrall, Melissa Poquette
  • Patent number: 8969071
    Abstract: An apparatus for passive sorting of microdroplets including a main flow channel, a flow stream of microdroplets in the main flow channel wherein the microdroplets have substantially the same diameter and wherein the flow stream of microdroplets includes first microdroplets having a first degree of stiffness and second microdroplets having a second degree of stiffness wherein the second degree of stiffness is different than the first degree of stiffness. A second flow channel is connected to the main flow channel for the second microdroplets having a second degree of stiffness. A separator separates the second microdroplets having a second degree of stiffness from the first microdroplets and directs the second microdroplets having a second degree of stiffness into the second flow channel.
    Type: Grant
    Filed: November 3, 2010
    Date of Patent: March 3, 2015
    Assignees: Lawrence Livermore National Security, LLC, The Regents of the University of California
    Inventors: Neil Reginald Beer, Abraham P. Lee, Andrew C. Hatch, Jeffrey S. Fisher
  • Patent number: RE46318
    Abstract: Colored fluids for electrowetting, electro fluidic, or electrophoretic devices, and the devices themselves, are disclosed. The colored fluid can include a nonaqueous polar solvent having (a) a dynamic viscosity of 0.1 cP to 50 cP at 250 C, (b) a surface tension of 25 dynes/cm to 55 dynes/cm at 250 C, and (c) an electrowetting relative response of 40% to 80%. Such colored fluids further include a colorant selected from a pigment and/or a dye. In another embodiment, the colored fluid can include a non-polar solvent and an organic colorant selected from a pigment and/or a dye. Such colored fluids can be black in color and have a conductivity from 0 pS/cm to 5 pS/cm and a dielectric constant less than 3. The use of the colored fluids offers improvements in reliability, higher levels of chroma in the dispersed state, and the ability to achieve higher contrast ratios in display technologies.
    Type: Grant
    Filed: May 16, 2014
    Date of Patent: February 21, 2017
    Assignee: Sun Chemical Corporation
    Inventors: Jason C. Heikenfeld, Russell Schwartz, Lisa Clapp, April Milarcik, Stanislav G. Vilner