Capillary Electrophoresis Type Patents (Class 204/601)
  • Patent number: 11150214
    Abstract: The capillary electrophoresis apparatus according to the present invention can maintain the temperature in the longitudinal direction of each of a plurality of capillaries uniformly, such that the separation performance of the capillary electrophoresis apparatus can be stabilized, and the analysis performance can be improved.
    Type: Grant
    Filed: March 8, 2018
    Date of Patent: October 19, 2021
    Assignee: Hitachi High-Tech Corporation
    Inventors: Wataru Sato, Motohiro Yamazaki
  • Patent number: 11109171
    Abstract: A semiconductor device and its manufacturing method are presented. The manufacturing method includes providing a substrate structure; forming a first metal layer on the substrate structure; forming a second metal layer on the first metal layer; forming a first oxide layer on the second metal layer at a first temperature; and conducting the remaining manufacturing processes including thermal processes at a second temperature that is higher than the first temperature. This method reduces the concentration of the first metal diffused into the surface of the second metal layer during the thermal processes, thus reducing the amount of the oxide of the first metal formed on the surface of the second metal layer. Therefore, it is beneficial to the establishment of metal wire connections.
    Type: Grant
    Filed: December 30, 2019
    Date of Patent: August 31, 2021
    Inventors: Yijun Chen, Yu Hua, Kuanchieh Yu, Chao Wang, Shan Zhang
  • Patent number: 11045806
    Abstract: The present invention provides an integrated type microfluidic electrochemical biosensor system for rapid biochemical analysis and the usage of the system. The system comprising: a continuous feeding unit for sequentially conveying lead eluent, sample solution, sample eluent, signal probe solution, signal probe eluent and electrochemical detection buffer solution; a microfluidic chip consists of one or more micro-channel network, the microfluidic chip covers the electrode array to form a channel system, capture probes which have interaction with the said sample solution fixed on the surface of the electrode array, said channel system is connected with the continuous feed unit; and a power system for providing power to said continuous feeding unit.
    Type: Grant
    Filed: June 8, 2020
    Date of Patent: June 29, 2021
    Assignee: HANGZHOU JINCHENGYIBANG TECHNOLOGY CO. LTD
    Inventors: Chunhai Fan, Fan Yang, Xiaolei Zuo, Qing Huang
  • Patent number: 11041834
    Abstract: A chromatographic device includes a primary channel having a cross-sectional area and characteristic length such that analyte travel within the primary channel is substantially convective. A plurality of secondary channels each having a cross-sectional area and characteristic length such that analyte flow into and out of a secondary channel is substantially diffusive, each of the plurality of secondary channels having an entrance in fluidic communication with the primary channel wherein the entrance intersects the primary channel.
    Type: Grant
    Filed: March 5, 2019
    Date of Patent: June 22, 2021
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Abhijit Tarafder, Moon Chul Jung, Edouard S. P. Bouvier
  • Patent number: 11022598
    Abstract: Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.
    Type: Grant
    Filed: October 3, 2017
    Date of Patent: June 1, 2021
    Assignee: ABBOTT LABORATORIES
    Inventors: Jeffrey B. Huff, Mark A. Hayden, Peter J. Karabatsos, Andrew S. Schapals, Anthony S. Muerhoff, M. Felicia Bogdan, Thomas Leary, Shelley R. Holets-McCormack, Sophie Laurenson, Andrew T. Fischer, Richard Haack, Stefan Hershberger, Dustin House, Lei Qiao, M. Shawn Murphy, Mark R. Pope, Edna M. Prieto-Ballengee, QiaoQiao Ruan, Pathik Soni, Sergey Tetin, Lyle Yarnell
  • Patent number: 10926717
    Abstract: A vehicle with an integrated portable audio system includes an interior body defining an interior passenger compartment and a docking station having a back wall, an upper wall, a lower wall, and side walls that define a receiving compartment. A wireless speaker is removably dockable with the docking station. When docked with the docking station, the wireless speaker is configured to connect to an audio system of the vehicle. When removed from the docking station, the wireless speaker is portable and configured to connect to a peripheral device for playing audio media on the wireless speaker.
    Type: Grant
    Filed: October 23, 2019
    Date of Patent: February 23, 2021
    Assignee: FCA US LLC
    Inventors: Matthew A Berg, Coby S Clark
  • Patent number: 10919008
    Abstract: The present invention generally relates to systems and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet contained within a fluidic channel, or a fluid may be injected into a fluidic channel to create a droplet. In some embodiments, electrodes may be used to apply an electric field to one or more fluidic channels, e.g., proximate an intersection of at least two fluidic channels. For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc.
    Type: Grant
    Filed: July 25, 2017
    Date of Patent: February 16, 2021
    Assignee: President and Fellows of Harvard College
    Inventors: David A. Weitz, Adam R. Abate, Tony Hung, Pascaline Mary
  • Patent number: 10917963
    Abstract: A device may include a temperature controlled chamber. The temperature controlled chamber may be coupled to a plurality of strengthening coated capillary tubes. The strengthening coated capillary tubes may support the temperature controlled chamber and provide thermal insulation to the temperature controlled chamber.
    Type: Grant
    Filed: August 20, 2019
    Date of Patent: February 9, 2021
    Assignee: Palo Alto Research Center Incorporated
    Inventor: Yunda Wang
  • Patent number: 10866246
    Abstract: Devices and methods for characterization of samples are provided. Samples may comprise one or more analytes. Some methods described herein include performing enrichment steps on a device. Some methods described herein include performing mobilization of analytes. Analytes may then be further processed and characterized.
    Type: Grant
    Filed: February 4, 2020
    Date of Patent: December 15, 2020
    Assignee: INTABIO, INC.
    Inventors: Scott Mack, Erik Gentalen
  • Patent number: 10712311
    Abstract: One embodiment of the invention provides: a microchip including a sample collection section and an analysis section, in which the sample collection section and the analysis section are imparted with both hydrophilicity and a positively-charged layer; an analysis system including the microchip; and a method of producing the microchip. The microchip includes: a sample collection section for collecting a sample; and an analysis section for analyzing the sample. In the microchip, a cationic polymer bonded with a hydrophilization substance is immobilized on inner walls of the sample collection section and the analysis section.
    Type: Grant
    Filed: October 19, 2017
    Date of Patent: July 14, 2020
    Assignee: ARKRAY, Inc.
    Inventors: Takanari Shigemitsu, Naotsugu Onuma
  • Patent number: 10690627
    Abstract: The present disclosure provides systems and methods for sample preparation, processing and analysis. Also provided in the present disclosure is a fully-integrated electrophoresis cartridge which has a small footprint and configured to removably engage with the system.
    Type: Grant
    Filed: October 21, 2015
    Date of Patent: June 23, 2020
    Assignee: IntegenX, Inc.
    Inventors: Alexander Kindwall, Chungsoo Charles Park, Fedor Ilkov, Stevan Jovanovich, David King, Tristan Robert Littlehale, David Grant Nilson, Rick Pittaro, Robert A. Schueren, Mattias Vangbo, Philip Justus Wunderle
  • Patent number: 10632465
    Abstract: The present invention relates to a contact-less priming system for loading a solution in a microfluidic device comprising: at least one microfluidic device, a pressure chamber configured to enclose said at least one microfluidic device, a pressurization unit fluidly connected to the pressure chamber and at least one closing member. The present invention also relates to a contact-less priming method for loading a solution in a microfluidic device.
    Type: Grant
    Filed: April 22, 2016
    Date of Patent: April 28, 2020
    Assignee: Stilla Technologies
    Inventors: Rémi Dangla, Étienne Fradet
  • Patent number: 10598919
    Abstract: Electrowetting-actuated optical shutters based on total internal reflection or beam steering. An electrowetting cell contains a conducting liquid and a non-conducting liquid configured to form a liquid-liquid interface extending to the inner walls of the cell. A beam of light is directed to the liquid-liquid interface at an angle near the total internal reflection angle of the interface. Voltage changes the shape of the liquid-liquid interface, without separating it from the inner walls of the cell. Thus, when depending on the voltage applied, the beam is either transmitted in part or substantially totally internal reflected.
    Type: Grant
    Filed: March 5, 2017
    Date of Patent: March 24, 2020
    Assignee: The Regents of the University of Colorado
    Inventors: Juliet T. Gopinath, Victor M. Bright, Andrew M. Jones
  • Patent number: 10556233
    Abstract: The invention is directed to a microfluidic device, which comprises distinct, parallel levels, including a first level and a second level. It further includes: a first microchannel, a second microchannel, and a node. This node comprises: an inlet port, a cavity, a via, and an outlet port. The cavity is formed on the first level and is open on a top side. The inlet port is defined on the first level; it branches from the first microchannel and communicates with the cavity through an ingress thereof. The outlet port, branches to the second microchannel on the second level. The via extends from the bottom side of the cavity, down to the outlet port, so the cavity may communicate with the outlet port. In addition, the cavity comprises a liquid blocking element to prevent an aqueous liquid filling the inlet port to reach the outlet port.
    Type: Grant
    Filed: June 23, 2017
    Date of Patent: February 11, 2020
    Assignee: International Business Machines Corporation
    Inventors: Emmanuel Delamarche, Onur Gökçe
  • Patent number: 10514358
    Abstract: The invention relates to a method which realizes a two-dimensional separation of ionic species on the basis of the online coupling of ion chromatography (IC) and capillary electrophoresis (CE). A device for IC×CE coupling, its implementation in terms of two alternatives, the connection to a mass spectrometric detector, and corresponding application are described.
    Type: Grant
    Filed: April 23, 2015
    Date of Patent: December 24, 2019
    Assignee: UNIVERSITÄT REGENSBURG
    Inventors: Frank-Michael Matysik, Andrea Beutner, Jonas Mark, Sven Kochmann
  • Patent number: 10509032
    Abstract: An assay method and device can perform at least one (e.g., at least two) assays on a single aliquot of a sample liquid. The device can mix a sample liquid with assay reagents including magnetically susceptible particles. The device is configured to create a sample liquid-air interface with the sample liquid. The magnetically susceptible particles can be located (via an applied magnetic field) at the liquid-air interface when a second liquid contacts the interface to form a liquid-liquid interface. The magnetic particles travel across the liquid-liquid interface to the second liquid. The magnetically susceptible particles are configured to trans-port an analyte across the interface into the second liquid. An assay for the analyte is performed in the second liquid. An assay for another analyte can also be performed in the sample liquid.
    Type: Grant
    Filed: February 27, 2017
    Date of Patent: December 17, 2019
    Assignee: ALERE SWITZERLAND GMBH
    Inventors: Phillip Lowe, Steven Alexander Keatch, Steven Howell, Claus Marquordt, Ruth Polwart, Alan Kenneth Thomson
  • Patent number: 10470292
    Abstract: A device may include a temperature controlled chamber. The temperature controlled chamber may be coupled to a plurality of strengthening coated capillary tubes. The strengthening coated capillary tubes may support the temperature controlled chamber and provide thermal insulation to the temperature controlled chamber.
    Type: Grant
    Filed: August 22, 2017
    Date of Patent: November 5, 2019
    Assignee: PALO ALTO RESEARCH CENTER INCORPORATED
    Inventor: Yunda Wang
  • Patent number: 10301666
    Abstract: Contemplated methods and devices are drawn to preparation and analysis of analytes from biological samples. In a preferred embodiment the analytes are nucleic acids that are both released from biological compartments present in the sample and fragmented through the use of a voltage potential applied to a pair of electrodes. The nucleic acids thus prepared are subsequently characterized.
    Type: Grant
    Filed: January 17, 2017
    Date of Patent: May 28, 2019
    Assignee: General Atomics
    Inventors: Shana O. Kelley, Susan Bortolin, Reginald James McKenzie Orton, Stefan Christopher Wiechula
  • Patent number: 10285316
    Abstract: The movement time of a board is shortened in an electronic component mounting device that performs heating and component mounting. Heating and component mounting are performed as a board is moved in the direction of arrow A; the board is moved by an in-conveyor device in the heating region, and by a shuttle device on rails in the component mounting region. When comparing chains with rails, the friction generated between the board is smaller with chains, thus the movement speed of the in-conveyor is faster. This means, compared to a case in which the board is moved by the rails and shuttle device in both the heating region and the component mounting region, the movement time of the board is shorter. Also, because there are no chains below the rails, the movable range of a flow tank is larger, and the component mountable region is larger.
    Type: Grant
    Filed: October 21, 2013
    Date of Patent: May 7, 2019
    Assignee: FUJI CORPORATION
    Inventors: Katsunori Tanaka, Tsuyoshi Hamane, Kazuya Degura
  • Patent number: 10180676
    Abstract: To provide a microchip-type optical measuring apparatus which is able to automatically perform position adjustment of a microchip with respect to an optical axis of laser with high accuracy. A microchip-type optical measuring apparatus includes an irradiation detection unit which detects light generated by irradiating a microchip with laser, a position adjustment unit which changes a relative position of the microchip with respect to the irradiation detection unit, and a control unit which outputs a movement signal for a position in which an integrated value or an average value of a detected intensity of the light in a preset region becomes high to the position adjustment unit.
    Type: Grant
    Filed: January 19, 2018
    Date of Patent: January 15, 2019
    Assignee: Sony Corporation
    Inventors: Yosuke Muraki, Fumitaka Otsuka
  • Patent number: 10126294
    Abstract: In one embodiment, the invention is to a sample metering device, comprising a sample holding chamber oriented between a sample entry port and a sample isolation unit and having a diluent introduction port disposed therebetween for introduction of a diluent into the sample holding chamber. The volume within the sample holding chamber between the diluent introduction port and the sample isolation unit defines a metered volume of a sample for analysis. In another embodiment, the invention is to an apparatus and method for rapid determination of analytes in liquid samples by various assays including immunoassays incorporating a sample dilution feature, preferably suitable for low range sample dilution, and preferably capable of being used in the point-of-care diagnostic field.
    Type: Grant
    Filed: May 14, 2015
    Date of Patent: November 13, 2018
    Assignee: Abbott Point of Car Inc.
    Inventor: Cary James Miller
  • Patent number: 10113947
    Abstract: According to one embodiment, a semiconductor analysis chip for detecting a particle in a sample liquid includes a semiconductor substrate, a first flow channel provided in a surface portion of the semiconductor substrate, a second flow channel provided in a surface portion of the semiconductor substrate, part of the second flow channel contacting or intersecting the first flow channel, a micropore provided in a contact portion or an intersection of the first and second flow channels, and configured to permit the particle to pass therethrough, a first electrode provided in the first flow channels, a second electrode provided in the second passage, and a third electrode provided in the first flow channel downstream of the first electrode.
    Type: Grant
    Filed: March 2, 2016
    Date of Patent: October 30, 2018
    Assignee: KABUSHIKI KAISHA TOSHIBA
    Inventors: Kentaro Kobayashi, Hiroshi Hamasaki, Naofumi Nakamura
  • Patent number: 10058818
    Abstract: Present embodiments provide an energy-efficient separator having at least two chambers fluidically isolated by a gas permeable membrane for the removal of dissolved gases from a sample which, in some aspects, enables cost-effective monitoring of liquid samples to be performed remotely and automatically, and in other aspects is suited for various other applications where removal and/or regulation of gases in a sample are desirable.
    Type: Grant
    Filed: May 27, 2015
    Date of Patent: August 28, 2018
    Assignee: UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION, INC.
    Inventors: Mohamed M. Marei, Richard P. Baldwin, Thomas J. Roussel, Jr., Robert S. Keynton
  • Patent number: 9968930
    Abstract: A microfluidic product utilizing gradient surface energy coatings for fluid control comprising a plurality of fluid passages wherein at least one fluid passage comprises a coating configured to control liquid flow wherein the coating configured to control liquid flow comprises a gradient surface energy coating from a proximal location to a distal location on a surface of the fluid passage. The product can include uniform regions and surface gradient regions in the same passage. Coating compositions and product dimensions can be selected to provide control over different flow properties including fluid velocity, reduction and acceleration of fluid flow, and starting and stopping fluid flow.
    Type: Grant
    Filed: March 26, 2014
    Date of Patent: May 15, 2018
    Inventor: Brian David Babcock
  • Patent number: 9927396
    Abstract: Aspects of the innovations presented herein relate to improved systems that in some embodiments perform capillary electrophoresis (CE) and CE in conjunction with electrospray ionization (ESI) as an input to a mass spectrometry system (MS). Some embodiments use a high voltage isolated CE power supply that is configured to float on the high voltage output of an ESI-MS power supply, with a protective resistance in the ESI-MS path, as well as DC/DC converter isolation and communication system isolation for the isolated CE power supply. Some embodiments additionally use a cartridge assembly integrating separation and conductive fluid capillaries with fluid cooling and protective retractable housings for the capillary end portions and for the ESI output. The protective housing may further be used with an adapter for interfacing with different MS systems.
    Type: Grant
    Filed: October 26, 2012
    Date of Patent: March 27, 2018
    Assignee: DH Technologies Development Pte. Ltd.
    Inventors: Brian D. Peterson, Dale Alan Owens, Peter S. Kotowski, Sunil S. Deliwala, Craig A. Hudson, James H. Reyes, Clive Cheung, Tom Tran, Anna X. Lou, Jane Luo, Jean-Marc Rene Lucien Busnel, Chitra K. Ratnayake
  • Patent number: 9927399
    Abstract: A method for analyzing a protein and a peptide, includes: providing a capillary for isoelectric focusing; providing a capillary device for separation and analysis having the capillary and a solid-phase extraction column being unified as a single tube-like structure; providing an electrophoresis instrument having the capillary device and the mechanism regulating the pressure difference at both ends of the capillary device; introducing a sample containing a target protein or peptide into the solid-phase extraction column to let the target protein or peptide be adsorbed on the column, and filling the capillary device with a carrier ampholyte solution; starting separation by isoelectric focusing after eluting the target protein or peptide by filling the solid-phase extraction column with electrode solution or acid or base solution, or after firstly eluting the target protein or peptide with an eluting solution containing carrier ampholyte and secondly filling the solid-phase extraction column with electrode solut
    Type: Grant
    Filed: October 14, 2014
    Date of Patent: March 27, 2018
    Assignees: NICHIEI INDUSTRY CO., LTD.
    Inventors: Kiyohito Shimura, Toshihiko Nagai, Shuuichi Fukuhara, Yoshi-ichi Seto
  • Patent number: 9915935
    Abstract: To provide a microchip-type optical measuring apparatus which is able to automatically perform position adjustment of a microchip with respect to an optical axis of laser with high accuracy. A microchip-type optical measuring apparatus includes an irradiation detection unit which detects light generated by irradiating a microchip with laser, a position adjustment unit which changes a relative position of the microchip with respect to the irradiation detection unit, and a control unit which outputs a movement signal for a position in which an integrated value or an average value of a detected intensity of the light in a preset region becomes high to the position adjustment unit.
    Type: Grant
    Filed: January 28, 2013
    Date of Patent: March 13, 2018
    Assignee: Sony Corporation
    Inventors: Yosuke Muraki, Fumitaka Otsuka
  • Patent number: 9863904
    Abstract: A method of analyzing a molecule is disclosed. A voltage source is selectively connected to or disconnected from a capacitor using a switch controlled by a reset signal. A charge is stored in a capacitor when the voltage source is connected to the capacitor. The capacitor is discharged through a nanopore in a membrane when the voltage source is disconnected from the capacitor. A duty cycle of the reset signal is determined such that the voltage source and the capacitor is connected for at least a one tenth portion of a reset signal period and disconnected for a remaining portion of the reset signal period, such that a voltage across the nanopore is maintained at a higher level during the portion of the reset signal period in which the connection is maintained than during the remaining portion of the reset signal period in which the connection is not maintained.
    Type: Grant
    Filed: December 16, 2015
    Date of Patent: January 9, 2018
    Assignee: Genia Technologies, Inc.
    Inventors: Roger J. A. Chen, Hui Tian, Bill Maney
  • Patent number: 9683994
    Abstract: The present invention provides novel methods for performing pulsed field mobility shift assays in microfluidic devices. In particular, the methods of the invention utilize differences between electrophoretic mobilities (e.g., as between reactants and products, especially in non-fluorogenic reactions) in order to separate the species and thus analyze the reaction.
    Type: Grant
    Filed: July 9, 2012
    Date of Patent: June 20, 2017
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Andrea W. Chow, John C. Owicki, J. Wallace Parce
  • Patent number: 9651527
    Abstract: An interface for a mass spectrometer is disclosed comprising a microfluidic substrate, tile or cartridge 1 comprising a liquid chromatography separation column and an electrospray emitter 2. A counter electrode 4 is arranged downstream of a tip of the electrospray emitter 2 and is arranged and adapted to direct ions towards an atmospheric pressure interface or ion inlet aperture 5 of a mass spectrometer.
    Type: Grant
    Filed: December 2, 2015
    Date of Patent: May 16, 2017
    Assignee: Micromass UK Limited
    Inventors: David S. Douce, John Chipperfield, Stevan Bajic, James Murphy, David Gordon
  • Patent number: 9595430
    Abstract: A self-assembled engineered lattice of nanometer-scale silica particles, or other suitable particles generally resembling regularly-sized spheres, is configured in a separation bed for electrophoresis, isoelectric focusing, chromatography, or other voltage-induced separation of analytes. After separation, the analytes are immobilized on the separation bed and then ionized using matrix-assisted laser desorption/ionization (MALDI) for use with a mass spectrometer. The nanoparticles can be coated with polymers that activate to immobilize the analytes or assist with MALDI. The separation can occur in two dimensions.
    Type: Grant
    Filed: March 25, 2015
    Date of Patent: March 14, 2017
    Assignees: LI-COR, INC., Purdue Research Foundation
    Inventors: Michael D. Furtaw, Jon P. Anderson, Mary J. Wirth, Robert E. Birdsall, Yimin Hua, Zhaorui Zhang
  • Patent number: 9580679
    Abstract: Disclosed herein are methods and systems for use in preparing a sample. The methods and systems may be used for lysing one or more structures in a sample (e.g., cells, viral particles, etc.). The methods and compositions may comprise a microfluidic chip or use thereof. The microfluidic chips disclosed herein may comprise (a) a substrate comprising a chamber, wherein at least one mechanical element may be located within the chamber; (b) a thermal element in contact with the chamber; and (c) at least one aperture within the surface of the substrate, wherein the aperture may be configured to insulate the chamber.
    Type: Grant
    Filed: September 20, 2013
    Date of Patent: February 28, 2017
    Assignee: California Institute of Technology
    Inventors: Samuel Njoroge, John Gorman, George Maltezos, Axel Scherer
  • Patent number: 9566504
    Abstract: The present invention relates to an air cleaner for removing air pollutants from an air stream, for instance such as the cleaning exhaust/intake gas of an amusement machine, said air cleaner comprising a filter device including at least one filter layer (4a, 4b) held by a filter holder (5) in said airflow substantially perpendicular to a main flow direction thereof and including a plurality of preferably stick-shaped filter elements (6) neighbouring each other. In accordance with the present invention, the filter device has no closed surrounding along the circumference of the filter layers, but provides for an open side along at least a portion of the periphery of the filter layer. At least one circumferential side section (7, 8) of the filter layer parallel to or tangential to the longitudinal axis of an outermost filter element is formed as an open side free of flow control elements surrounding the filter layer.
    Type: Grant
    Filed: February 26, 2014
    Date of Patent: February 14, 2017
    Assignees: NOVOMATIC AG, DEXWET USA LLC
    Inventors: Christoph Bichl, Heinz Winkler, Alois Homer, Erich Petein, Maximillian Vojta
  • Patent number: 9539574
    Abstract: This invention provides methods and systems for injection of analytes into a separation channel for resolution and detection. Samples can be preconditioned and concentrated by isotachophoresis (ITP) before the injection is triggered by a detected voltage event. Separation of analytes from other sample constituents can be enhanced using skewing channel ITP.
    Type: Grant
    Filed: October 11, 2010
    Date of Patent: January 10, 2017
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Charles Park, Persefoni Kechagia, Michael Spaid, Morten Jensen, Irina G. Kazakova, Josh Molho
  • Patent number: 9535033
    Abstract: The analysis method allows analysis of samples with high sensitivity, irrespective of interelectrode distance. The method includes: a step of applying a voltage between a first electrode pair such that an electric field is formed in a direction intersecting a migration direction of a sample; a step of placing a solution, including an electrochemically active molecule that produces a redox reaction at the electrode pair, between the first electrode pair; a step of causing the sample to migrate; and a step of measuring an amount of change in current flow between the first electrode pair.
    Type: Grant
    Filed: August 2, 2013
    Date of Patent: January 3, 2017
    Assignee: QUANTUM BIOSYSTEMS INC.
    Inventors: Tomoji Kawai, Takahito Ohshiro, Masateru Taniguchi
  • Patent number: 9494553
    Abstract: An electrophoresis chip that can be small and simple and that can analyze a sample with high accuracy is provided. The electrophoresis chip includes an upper substrate 4, a lower substrate 1, an introduction reservoir 2a, a recovery reservoir 2b and a capillary channel for sample analysis 3x. The introduction reservoir 2a and the recovery reservoir 2b are formed in the lower substrate 1. The introduction reservoir 2a and the recovery reservoir 2b are in communication with each other via the capillary channel for sample analysis 3x. The introduction reservoir 2a receives a sample to be measured. The sample is electrophoretically introduced directly into the capillary channel for sample analysis 3x by creating a potential difference between the introduction reservoir 2a and the recovery reservoir 2b, and is also analyzed in the capillary channel for sample analysis 3x during the separation of the sample while the sample is being continuously supplied.
    Type: Grant
    Filed: April 28, 2008
    Date of Patent: November 15, 2016
    Assignees: National Institute of Advanced Industrial Science and Technology, ARKRAY, Inc.
    Inventors: Yoshihide Tanaka, Yusuke Nakayama, Satoshi Yonehara
  • Patent number: 9470724
    Abstract: A method and a system are shown for attachment of leads for the electrical sensing of the voltage on conductive structures; connections attached per this invention have the valuable property of very low thermoelectric errors (due to Seebeck effect), among several other beneficial properties. The described method can be applied in the factory setting as well as in the field. This system is especially suitable for applications with high-precision resistive shunts utilized in the measurements of the electric current.
    Type: Grant
    Filed: August 12, 2015
    Date of Patent: October 18, 2016
    Assignee: Sendyne Corporation
    Inventor: Victor Marten
  • Patent number: 9360403
    Abstract: The present invention provides a device and methods of use thereof in concentrating a species of interest and/or controlling liquid flow in a device. The methods make use of a device comprising a fluidic chip comprising a planar array of channels through which a liquid comprising a species of interest can be made to pass with at least one rigid substrate connected thereto such that at least a portion of a surface of the substrate bounds the channels, and a high aspect ratio ion-selective membrane is embedded within the chip, attached to at least a portion of the channels. The device comprises a unit to induce an electric field in the channel and a unit to induce an electrokinetic or pressure driven flow in the channel.
    Type: Grant
    Filed: April 12, 2013
    Date of Patent: June 7, 2016
    Assignee: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Jongyoon Han, Sung Jae Kim
  • Patent number: 9330612
    Abstract: A device cover includes a protective layer and a display layer. The display layer includes a plurality of display pixels. Each display pixel includes a first fluid having a first color characteristic and a second fluid having a second color characteristic.
    Type: Grant
    Filed: July 5, 2014
    Date of Patent: May 3, 2016
    Inventor: Robert Reid
  • Patent number: 9193748
    Abstract: The present invention concerns the use of methods and compositions for the isolation of small RNA molecules (100 nucleotides or fewer), such as microRNA and siRNA molecules. Such molecules are routinely lost in commonly used isolation procedures and therefore the present invention allows for a much higher level of enrichment or isolation of these small RNA molecules.
    Type: Grant
    Filed: February 27, 2013
    Date of Patent: November 24, 2015
    Assignee: Applied Biosystems, LLC
    Inventor: Richard Conrad
  • Patent number: 9168527
    Abstract: An electrokinetic fluidic system (100, 100?, 100?) for controlling liquid flow in e.g. a lab-on-a-chip system (200) comprising a first and a second electrode (10, 10?) said first and second electrode comprising a polymer based or oxide based conductive, electrochemically active electrode material, said electrode material being adapted to be subjected to an electrochemical reaction when in use in said electrokinetic fluid system (100).
    Type: Grant
    Filed: February 22, 2011
    Date of Patent: October 27, 2015
    Assignee: LunaMicro AB
    Inventors: Nathaniel D. Robinson, Per Erlandsson
  • Patent number: 9110044
    Abstract: An embodiment of the present invention relates to a system for the integrated and automated analysis of DNA or protein, including a single-use cartridge, an analysis device comprising a control device, and devices for capturing and processing signals. An embodiment of the present invention relates, in particular, to the control device for carrying out a completely automatic process and evaluation of molecular diagnostic analysis via single-use cartridges (Lab-on-a-Chip). The first devices are provided for controlling an analysis process which occurs in the cartridge, subsequently the displacement and the thermostatisation of liquids, and the second devices are provided for processing the signals which are obtained during the analysis. The first and the second devices are synchronized in such a manner that the analysis process of the sample can be carried out in a totally integrated manner thus producing an immediate result.
    Type: Grant
    Filed: May 22, 2006
    Date of Patent: August 18, 2015
    Assignee: Boehringer Ingelheim Vetmedica GmbH
    Inventors: Walter Gumbrecht, Peter Paulicka
  • Patent number: 9052309
    Abstract: The invention is to devices and methods for rapid determination of analytes in liquid samples. The devices and methods incorporate a sample dilution feature and multiple immunosensors for performing a ratiometric immunoassay on a first analyte and a second analyte, for example, hemoglobin and hemoglobin A1c or albumin and glycosylated albumin. The devices are preferably capable of being used in the point-of-care diagnostic field.
    Type: Grant
    Filed: December 1, 2011
    Date of Patent: June 9, 2015
    Assignee: Abbott Point of Care Inc.
    Inventor: Cary James Miller
  • Publication number: 20150144489
    Abstract: A disposable cartridge used in a digital microfluidics system has a bottom layer with first hydrophobic surface, a rigid cover plate with second hydrophobic surface, and a gap there-between. The bottom layer is a flexible film on an uppermost surface of a cartridge accommodation site of a system, attracted to and spread over the uppermost surface by an underpressure. A lower surface of the plate and the flexible bottom layer are sealed to each other. The assembled cartridge is removed from the cartridge accommodation site in one piece and potentially includes samples and processing fluids. The system has a base unit and a cartridge accommodation site with an electrode array of individual electrodes and a central control unit for controlling selection of individual electrodes and for providing these electrodes with individual voltage pulses for manipulating liquid droplets within the gap by electrowetting.
    Type: Application
    Filed: January 6, 2014
    Publication date: May 28, 2015
    Applicant: TECAN TRADING AG
    Inventors: Daniel Hoffmeyer, Tiffany Lay, Travis Lee, Marc N. Feiglin, Werner Halg, Anne R. Kopf-Sill
  • Publication number: 20150136602
    Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.
    Type: Application
    Filed: November 24, 2014
    Publication date: May 21, 2015
    Inventors: Stevan B. Jovanovich, William D. Nielsen, David S. Cohen, Michael Recknor, Mattias Vangbo, Ezra Van Gelder, Lars Majlof, Omar El-Sissi
  • Patent number: 9034163
    Abstract: An electrode assembly for capillary electrophoresis (CE) comprises a manifold (310), a connector (305) a sheath (300), and a seal (325). A capillary tube (100) passes through the manifold, the connector, the sheath, and the seal, stopping just beyond the end of the sheath. The sheath is fillable with water (330) or another fluid that cools the capillary tube in the vicinity of the electrode, thereby preventing degradation of a sample due to heat. The sheath may be metal or plastic with a metal sleeve electrode on its exterior. The sheath is sufficiently strong to penetrate a rubber or other pierceable cap on a vial. The manifold and connector incorporate an air path (605, 312, 307) so that when the electrode is fully inserted into a vial, the contents (650) of the vial are at atmospheric pressure (or another applied pressure or vacuum).
    Type: Grant
    Filed: November 20, 2012
    Date of Patent: May 19, 2015
    Inventor: Tiansong Wang
  • Patent number: 9034161
    Abstract: The present invention provides methods, devices, and kits for separating and selecting top sperm from a sperm sample of a subject. In one aspect, for example, such a method can include removing a portion of negatively charged protein from sperm in the sperm sample, immobilizing the sperm, electrophoretically separating the sperm, and selecting mature sperm based on electromotility properties.
    Type: Grant
    Filed: August 23, 2013
    Date of Patent: May 19, 2015
    Inventors: Douglas T. Carrell, Luke Simon
  • Publication number: 20150129427
    Abstract: The present invention relates to a process for the extraction of analyte compounds from a sample comprising one or more analytes in a donor phase into an acceptor phase, comprising the steps of: a) providing an electrically conductive donor phase comprising the compounds in a first electrically conductive solvent or solvent blend, and an electrode arranged in electrically conductive contact with the donor phase, b) providing an electrically conductive acceptor phase in electrically conductive contact with a second electrode; and c) providing an insulator phase in fluid communication with at least one of the donor phase and the acceptor phase, wherein the insulator phase is immiscible with the donor phase and/or the acceptor phase, and d) (d) applying an electrical field between the first and the second electrode.
    Type: Application
    Filed: April 19, 2013
    Publication date: May 14, 2015
    Inventors: Robert-Jan Raterink, Peter Lindenburg, Thomas Hankemeier
  • Publication number: 20150122651
    Abstract: A method of generating droplets of a dispersed phase fluid in a continuous phase fluid includes flowing the dispersed phase fluid and the continuous phase fluid to a channel junction of at least one dispersed phase channel and at least one continuous phase channel, applying at least one alternating voltage to at least two electrodes so that an alternating electric field is created at the channel junction, and generating the droplets of the dispersed phase fluid in the continuous phase fluid flowing in an output channel of the channel junction, wherein the dispersed phase fluid and the continuous phase fluid are electrically insulated from the at least two electrodes. Furthermore, a microfluidic device is described, which is configured for generating droplets of a dispersed phase fluid in a continuous phase fluid.
    Type: Application
    Filed: March 30, 2012
    Publication date: May 7, 2015
    Inventors: Jean-Christophe Baret, Say Hwa Tan, Benoit Semin
  • Patent number: RE46318
    Abstract: Colored fluids for electrowetting, electro fluidic, or electrophoretic devices, and the devices themselves, are disclosed. The colored fluid can include a nonaqueous polar solvent having (a) a dynamic viscosity of 0.1 cP to 50 cP at 250 C, (b) a surface tension of 25 dynes/cm to 55 dynes/cm at 250 C, and (c) an electrowetting relative response of 40% to 80%. Such colored fluids further include a colorant selected from a pigment and/or a dye. In another embodiment, the colored fluid can include a non-polar solvent and an organic colorant selected from a pigment and/or a dye. Such colored fluids can be black in color and have a conductivity from 0 pS/cm to 5 pS/cm and a dielectric constant less than 3. The use of the colored fluids offers improvements in reliability, higher levels of chroma in the dispersed state, and the ability to achieve higher contrast ratios in display technologies.
    Type: Grant
    Filed: May 16, 2014
    Date of Patent: February 21, 2017
    Assignee: Sun Chemical Corporation
    Inventors: Jason C. Heikenfeld, Russell Schwartz, Lisa Clapp, April Milarcik, Stanislav G. Vilner