Gel Filled Patents (Class 204/605)
  • Patent number: 10060903
    Abstract: A method of exporting measurements of a nanopore sensor on a nanopore based sequencing chip is disclosed. An electrical characteristic associated with the nanopore sensor is measured. The electrical characteristic associated with the nanopore sensor is processed. A summary for the electrical characteristic and one or more previous electrical characteristics is determined. The summary for the electrical characteristic and the one or more previous electrical characteristics are exported. Determining the summary includes determining that the electrical characteristic and at least a portion of the one or more previous electrical characteristics correspond to a base call event at the nanopore sensor. The summary represents the electrical characteristic and the at least a portion of the one or more previous electrical characteristics.
    Type: Grant
    Filed: November 5, 2014
    Date of Patent: August 28, 2018
    Assignee: Genia Technologies, Inc.
    Inventors: Roger J. A. Chen, Hui Tian, Santiago Fernandez-Gomez
  • Patent number: 9409357
    Abstract: Embodiments of the present invention provide devices, systems, and methods for microscale isoelectric fractionation. Analytes in a sample may be isolated according to their isoelectric point within a fractionation microchannel. A microfluidic device according to an embodiment of the invention includes a substrate at least partially defining a fractionation microchannel. The fractionation microchannel has at least one cross-sectional dimension equal to or less than 1 mm. A plurality of membranes of different pHs are disposed in the microchannel. Analytes having an isoelectric point between the pH of the membranes may be collected in a region of the fractionation channel between the first and second membranes through isoelectric fractionation.
    Type: Grant
    Filed: January 23, 2013
    Date of Patent: August 9, 2016
    Assignee: Sandia Corporation
    Inventors: Gregory J. Sommer, Anson V. Hatch, Ying-Chih Wang, Anup K. Singh
  • Patent number: 9140666
    Abstract: The invention is an improved multiplex capillary electrophoresis instrument or module with at least four and preferably six user-accessible vertically stacked drawers. An x-z stage moves samples from the user accessible drawers to the capillary array for analysis. A computer program allows users to add capillary electrophoresis jobs to a queue corresponding to the analysis of rows or plates of samples without stopping or interrupting runs in progress.
    Type: Grant
    Filed: May 14, 2012
    Date of Patent: September 22, 2015
    Assignee: Advanced Analytical Technologies, Inc.
    Inventors: Bruce R. Boeke, Martin Chris Foster, Thomas J. Kurt, Scott Stueckradt
  • Publication number: 20150122656
    Abstract: A mass separation device includes a transparent substrate and a plurality of small diameter cylindrically shaped orifices in the transparent substrate. The small diameter cylindrically shaped orifices include smooth wall surfaces and are not tapered. The small diameter cylindrically shaped orifices are drilled by photoacoustic compression and are clean and sharp and do not have ejecta mounds surrounding the orifice on the surface of the transparent substrate. The small diameter cylindrically shaped orifices in said transparent substrate are less than or equal to 1 ?m in diameter. The transparent substrate is glass and preferably is borosilicate glass.
    Type: Application
    Filed: November 3, 2014
    Publication date: May 7, 2015
    Applicant: ROFIN-SINAR TECHNOLOGIES INC.
    Inventor: S. ABBAS HOSSEINI
  • Publication number: 20150090595
    Abstract: A fitting (200) for providing a fluid connection between a capillary (202) and a fluidic conduit (204) of a fluidic component (30), the fitting (200) comprising a male piece (240) and a female piece (250) for connection with the male piece (240), wherein the male piece (240) comprises a housing (252) with a capillary reception (212) configured for receiving the capillary (202), wherein a part of the capillary (202) being received in the capillary reception (212) is circumferentially covered by a sleeve (214), an elastic biasing mechanism (206) being arranged at least partially within the housing (252), being configured for biasing the capillary (202) against the female piece (250) and being supported by the sleeve (214), and a locking mechanism (208) being arranged at least partially within the housing (252) and being configured for locking the capillary (202) to the fitting (200).
    Type: Application
    Filed: November 15, 2012
    Publication date: April 2, 2015
    Applicant: aGILENT TECHNOLOGIES , INC.
    Inventors: Claus Lueth, Darijo Zeko
  • Publication number: 20150001080
    Abstract: Embodiments of an electrophoresis device and methods for using the same in analyte separation applications are provided. Certain embodiments of the present disclosure include microfluidic devices having a single electrophoretic separation channel containing a separation medium. Systems according to embodiments of the present disclosure are configured to monitor analyte fronts moving through the electrophoretic separation channel in order to detect differentially migrating analytes, i.e., the systems are configured for moving boundary electrophoresis (MBE).
    Type: Application
    Filed: February 1, 2013
    Publication date: January 1, 2015
    Inventors: Amy E. Herr, Todd Duncombe
  • Publication number: 20140332382
    Abstract: Microfluidic devices and methods for using the same are provided. Embodiments include microfluidic devices that have a first separation region configured to separate a sample along a first directional axis based on a first property, and a second separation region in fluid communication with the first separation region and configured to separate the sample along a second directional axis based on a second property. Also provided are methods of using the devices as well as systems and kits that include the devices. The devices, systems and methods find use in a variety of different applications, including diagnostic and validation assays.
    Type: Application
    Filed: May 9, 2014
    Publication date: November 13, 2014
    Inventors: Amy E. Herr, Augusto Tentori
  • Publication number: 20140332383
    Abstract: Provided are devices that include a support, a free-standing polymeric separation medium associated with the support and configured to separate a sample along a directional axis, and a sample-loading element associated with the polymeric separation medium. Systems that include the devices, as well as methods of using the devices, are also provided. Embodiments of the present disclosure find use in a variety of different applications, including detecting whether an analyte is present in a fluid sample.
    Type: Application
    Filed: May 6, 2014
    Publication date: November 13, 2014
    Inventors: Amy E. Herr, Todd Duncombe
  • Publication number: 20140227772
    Abstract: The present invention relates to an immunoaffinity device for capturing one or more analytes present at high or low concentrations in simple or complex matrices. The device is designed as an integrated modular unit and connected to capillary electrophoresis or liquid chromatography for the isolation, enrichment, separation and identification of polymeric macromolecules, primarily protein biomarkers. The integrated modular unit includes an analyte-concentrator-microreaction device connected to a modified cartridge-cassette.
    Type: Application
    Filed: April 22, 2014
    Publication date: August 14, 2014
    Inventor: Norberto A. GUZMAN
  • Patent number: 8784631
    Abstract: Disclosed herein is a composition of a separation medium and method of its use for electrophoresis in bare channels, either capillaries or chips, with suppressed electroosmotic flow, wherein various forms of boric acid are adsorbed on the wall of said separation channel, efficiently suppressing zeta potential of the wall of said bare channel. A composition of a sieving separation medium for electrophoresis of DNA is disclosed. A composition of a sieving separation medium for electrophoretic size separation of proteins by SDS CSE is also disclosed. A composition of a separation medium for capillary electrophoresis without sieving is also disclosed.
    Type: Grant
    Filed: January 1, 2012
    Date of Patent: July 22, 2014
    Inventor: Vladislav Dolnik
  • Patent number: 8685220
    Abstract: The invention provides a capillary electrophoresis apparatus which can improve the operability and measuring speed. According to the invention, a sensor for identifying the type of sample containers is fixed at the position away from a capillary anode electrode. The sensor is made to be closer to the sample containers by moving a moving stage so that the sample containers disposed on the moving stage can be identified by the sensor. A fixing apparatus for fixing at least a pair of sample containers is provided on the moving stage.
    Type: Grant
    Filed: July 26, 2012
    Date of Patent: April 1, 2014
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Takeshi Ohura, Ryoji Inaba, Yoshitaka Kodama, Hiromi Yamashita, Manabu Akiba, Tomohiro Shoji
  • Patent number: 8603833
    Abstract: The present invention provides germania-silica-based sol-gel monoliths that form the solid-phase material for extraction, isolation, preconcentration, and separation of analytes. In one embodiment, the germania-silica-based sol-gel monolith can be used to coat surfaces of the inner walls of extraction and chromatographic columns. In a preferred embodiment, the sol-gel germania-silica monolith of the present invention is formed from hydrolysis and polycondensation of precursors comprising tetraethoxygermane, tetramethoxysilane, and polyethylene glycol. Also provided are methods of preparing the germania-silica-based sol-gel monolith of the present invention.
    Type: Grant
    Filed: June 3, 2011
    Date of Patent: December 10, 2013
    Assignee: University of South Florida
    Inventors: Abdul Malik, Erica B. Turner, Scott S. Segro
  • Patent number: 8585884
    Abstract: Disclosed herein is an apparatus for the separation of proteins, comprising a capillary isoelectric focusing unit (2) for primarily separating protein samples on the basis of pI; and a hollow fiber flow field flow fractionation unit (4), connected to one side of the capillary isoelectric focusing unit (2), for secondarily separating the protein samples. The apparatus allows proteins to be separated on the basis of pI and molecular weight without denaturation and can further be applied for the identification of proteins in conjunction with nanoflow liquid chromatography-electrospray ionization-tandem mass spectrometry after enzymatic digestion of the protein fractions.
    Type: Grant
    Filed: January 17, 2007
    Date of Patent: November 19, 2013
    Assignee: Industry-Academic Cooperation Foundation, Yonsei University
    Inventors: Myeong Hee Moon, Duk Jin Kang
  • Publication number: 20130277218
    Abstract: Devices and methods are provided for performing droplet-based solid phase processing steps on a digital microfluidic device. A solid phase material, which may be a porous solid phase material such as a porous polymer monolith is formed or located on a digital microfluidic element. The solid phase may be formed by an in-situ method in which the digital microfluidic array is actuated to transport a droplet of solid phase pre-cursor solution to a selected element on the array, and subsequently processed to form a solid phase on the array element. The integration of a solid phase material with a digital microfluidic array enables a wide range of applications including solid phase extraction and sample concentration.
    Type: Application
    Filed: October 3, 2011
    Publication date: October 24, 2013
    Applicant: THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO
    Inventors: Jared M. Mudrik, Hao Yang, Aaron R. Wheeler
  • Publication number: 20130248366
    Abstract: A method of amplifying nucleic acid includes providing a microfluidic device having a space therein. The space is filled with a gel medium. Reagents for carrying out a PCR reaction are supported within the matrix of the gel medium within the space. A nucleic acid containing sample is brought into contact with the gel medium. The sample having whole cells or cell lysate without any prior separation of the nucleic acid from other components of the cells. PCR amplification of nucleic acid from the sample is performed in the space using the PCR reagents.
    Type: Application
    Filed: September 21, 2011
    Publication date: September 26, 2013
    Applicant: THE UNIVERSITY OF HULL
    Inventors: Stephen John Haswell, Kirsty Jane Shaw, Peter Docker
  • Publication number: 20130213811
    Abstract: Systems and methods are provided for integrating the electrophoresis and blotting of samples. A capillary electrophoresis and blotting system allows concomitant electrophoretic separation and blotting to provide a rapid and simplified process. A microfluidic electrophoresis and blotting system provides electrophoretic separation in a microfluidic channel followed by electroblotting from the microfluidic channel to also provide a rapid and simplified process. These systems and methods can be used to assay smaller amounts of sample in less time than conventional processes, including conventional Western blotting techniques.
    Type: Application
    Filed: August 23, 2012
    Publication date: August 22, 2013
    Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN
    Inventors: Robert Kennedy, Gwendolyn Anderson
  • Patent number: 8512537
    Abstract: Various embodiments provide, for example, buffer compositions and/or sieving formulations useful in connection with electrophoresis instruments, such as capillary electrophoresis (CE) devices. In various embodiments, a buffer composition can include Bis-Tris, TAPS and/or TAPSO, and, optionally, a chelating agent, such as EDTA. Methods of separating samples containing bio-molecules, such as DNA or RNA, are also described.
    Type: Grant
    Filed: January 28, 2011
    Date of Patent: August 20, 2013
    Assignee: Applied Biosystems, LLC
    Inventors: Kevin J. Hacker, Karl O. Voss
  • Patent number: 8500980
    Abstract: A cost-effective multi-channel capillary gel-electrophoresis system for highly efficient, nucleic acid-based analysis, in particular HLA SSP (Human Leukocyte Antigen Sequence Specific Primer) typing applications. Twelve DNA samples are automatically injected, electrophoretically separated, detected and analyzed simultaneously by using a multiple usage and disposable multi-capillary gel-cartridge. Using commercially available DNA size markers as indicators, the system provides high resolving power with 12-channel separations in less than 1.5 minutes. The system can hold a total of 96 samples in a PCR plate that can automatically be analyzed within 25 minutes for a full plate of the HLA SSP kits.
    Type: Grant
    Filed: October 24, 2007
    Date of Patent: August 6, 2013
    Assignee: Qiagen Sciences, LLC
    Inventors: Varouj Amirkhanian, Ming-Sun Liu
  • Publication number: 20130078624
    Abstract: Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample.
    Type: Application
    Filed: September 26, 2011
    Publication date: March 28, 2013
    Applicant: Theranos, Inc., a Delaware Corporation
    Inventors: Elizabeth Holmes, Sunny Balwani
  • Publication number: 20130078625
    Abstract: Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample.
    Type: Application
    Filed: September 26, 2011
    Publication date: March 28, 2013
    Applicant: Theranos, Inc., a Delaware Corporation
    Inventors: Elizabeth Holmes, Joy Roy
  • Patent number: 8366900
    Abstract: The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided.
    Type: Grant
    Filed: March 16, 2010
    Date of Patent: February 5, 2013
    Assignee: Applied Biosystems, LLC
    Inventors: Karl O. Voss, Aldrich N. K. Lau
  • Patent number: 8349161
    Abstract: Bubbles can be removed regardless of an individual difference of a pump to fill an electrophoresis medium into a capillary. Of flow passages formed between an inner side surface of a container for accommodating the electrophoresis medium and a side surface of a plunger, one of the flow passages causing an electrophoresis medium to be easily stagnant is formed to have the cross-sectional area larger than the cross-sectional area of the other flow passage on the opposite side. In other words, the flow passage portion causing the electrophoresis medium to be easily stagnant is formed in such a manner as to increase a flow amount of the electrophoresis medium. This can eliminate a region having an extremely small amount of electrophoresis medium flow in the pump.
    Type: Grant
    Filed: April 6, 2010
    Date of Patent: January 8, 2013
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Takashi Gomi, Ryoji Inaba, Taro Nakazawa, Takeshi Ohura, Mari Kotoura
  • Patent number: 8329016
    Abstract: Disclosed is a novel microfluidic device enabling on-chip implementation of a two-dimensional separation methodology. Previously disclosed microscale immobilized pH gradients (IPG) are combined with perpendicular polyacrylamide gel electrophoresis (PAGE) microchannels to achieve orthogonal separations of biological samples. Device modifications enable inclusion of sodium dodecyl sulfate (SDS) in the second dimension. The device can be fabricated to use either continuous IPG gels, or the microscale isoelectric fractionation membranes we have also previously disclosed, for the first dimension. The invention represents the first all-gel two-dimensional separation microdevice, with significantly higher resolution power over existing devices.
    Type: Grant
    Filed: August 31, 2009
    Date of Patent: December 11, 2012
    Assignee: Sandia Corporation
    Inventors: Gregory J. Sommer, Anson V. Hatch, Anup K. Singh, Ying-Chih Wang
  • Publication number: 20120285832
    Abstract: The present invention relates to an immunoaffinity device for capturing one or more analytes present at high or low concentrations in simple or complex matrices. The device is designed as an integrated modular unit and connected to capillary electrophoresis or liquid chromatography for the isolation, enrichment, separation and identification of polymeric macromolecules, primarily protein biomarkers. The integrated modular unit includes an analyte-concentrator-microreaction device connected to a modified cartridge-cassette.
    Type: Application
    Filed: June 4, 2012
    Publication date: November 15, 2012
    Inventor: Norberto A. GUZMAN
  • Publication number: 20120267246
    Abstract: The present invention relates to novel methods and apparatus for improving the sensitivity of capillary zone electrophoresis (CZE). The invention particularly concerns devices comprising a channel that contains an in-line sol-gel column to concentrate samples being subjected to capillary zone electrophoresis, and the use of such devices to enhance the sensitivity of capillary zone electrophoresis.
    Type: Application
    Filed: April 20, 2012
    Publication date: October 25, 2012
    Inventors: Chitra Kumari Ratnayake, Michael Paul Henry
  • Patent number: 8293083
    Abstract: Provided are fabrication, characterization and application of a nanodisk electrode, a nanopore electrode and a nanopore membrane. These three nanostructures share common fabrication steps. In one embodiment, the fabrication of a disk electrode involves sealing a sharpened internal signal transduction element (“ISTE”) into a substrate, followed by polishing of the substrate until a nanometer-sized disk of the ISTE is exposed. The fabrication of a nanopore electrode is accomplished by etching the nanodisk electrode to create a pore in the substrate, with the remaining ISTE comprising the pore base. Complete removal of the ISTE yields a nanopore membrane, in which a conical shaped pore is embedded in a thin membrane of the substrate.
    Type: Grant
    Filed: November 18, 2010
    Date of Patent: October 23, 2012
    Assignee: University of Utah Research Foundation
    Inventors: Henry S White, Bo Zhang, Ryan J White, Eric N Ervin, Gangli Wang
  • Patent number: 8252162
    Abstract: The invention provides a capillary electrophoresis apparatus which can improve the operability and measuring speed. According to the invention, a sensor for identifying the type of sample containers is fixed at the position away from a capillary anode electrode. The sensor is made to be closer to the sample containers by moving a moving stage so that the sample containers disposed on the moving stage can be identified by the sensor. A fixing apparatus for fixing at least a pair of sample containers is provided on the moving stage.
    Type: Grant
    Filed: December 24, 2008
    Date of Patent: August 28, 2012
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Takeshi Ohura, Ryoji Inaba, Yoshitaka Kodama, Hiromi Yamashita, Manabu Akiba, Tomohiro Shoji
  • Patent number: 8241476
    Abstract: A novel on-line method is presented for the extraction and preconcentration of amino acids using a sol-gel coated column coupled to a conventional UV/vis detector. Extraction, stacking and focusing techniques are used in the preconcentration procedures. Sol-gel coatings are created by using N-Octadecyldimethyl[3(trimethoxysilyl)proply]ammonium chloride (C18-TMS) in the coating sol solutions. The resulting sol-gel coating carries a positive charge. For extraction, the pH of the samples is properly adjusted to impart a net negative charge to amino acids. A long plug of the sample is then passed through the sol-gel coated capillary to facilitate extraction via electrostatic interaction between the positively charged sol-gel coating and the negatively charged amino acid molecules. The focusing of the extracted amino acids is accomplished through desorption of the extracted amino acid molecules carried out by local pH change. The described procedure provides 150,000-fold enrichment effect for alanine.
    Type: Grant
    Filed: May 14, 2008
    Date of Patent: August 14, 2012
    Assignee: University of South Florida
    Inventors: Abdul Malik, Wen Li, David P. Fries
  • Patent number: 8221607
    Abstract: The invention provides compositions, methods and kits for high speed, high resolution of analytes by capillary electrophoresis starting with uncoated capillaries. The compositions comprise a sieving component, comprising a non-crosslinked acrylamide polymer, and a surface interaction component, comprising at least one uncharged and non-crosslinked water-soluble silica-adsorbing polymer. Methods for employing the novel compositions in capillary electrophoresis are provided. Kits comprising the novel compositions for use in the novel methods are also provided.
    Type: Grant
    Filed: March 5, 2004
    Date of Patent: July 17, 2012
    Assignee: Applied Biosystems, LLC
    Inventors: Karl O. Voss, Aldrich N. K. Lau
  • Patent number: 8182746
    Abstract: An electrophoresis apparatus is generally disclosed for sequentially analyzing a single sample or multiple samples having one or more analytes in high or low concentrations. The apparatus comprises a relatively large-bore transport capillary which intersects with a plurality of small-bore separation capillaries and includes a valve system. Analyte concentrators, having antibody-specific (or related affinity) chemistries, are stationed at the respective intersections of the transport capillary and separation capillaries to bind one or more analytes of interest. The apparatus allows the performance of two or more dimensions for the optimal separation of analytes. The apparatus may also include a plurality of valves surrounding each of the analyte concentrators to localize each of the concentrators to improve the binding of one or more analytes of interest.
    Type: Grant
    Filed: October 31, 2007
    Date of Patent: May 22, 2012
    Assignee: Princeton Biochemicals, Inc.
    Inventor: Norberto A. Guzman
  • Patent number: 8163152
    Abstract: A cost-effective multi-channel capillary gel-electrophoresis system for highly efficient, high speed, high throughput, carbohydrate analysis. In one aspect of the present invention, a high-performance capillary electrophoresis analyzer system has been optimized for carbohydrate analysis application. The system uses a multiplexed/time-staggered fluorescence type detection mechanism, with an integrated fiber optic array-based technology and a novel disposable gel-cartridge. Twelve carbohydrate samples are automatically injected, electrophoretically separated, detected and analyzed simultaneously by using a multiple usage and disposable multi-capillary gel-cartridge. Using commercially available labeling agent such as APTS (representing 8-Aminopyrene-1,3,6-trisulfonic acid, trisodium salt) as an indicator, the capillary electrophoresis-based carbohydrate system/analyzer provides high resolving power in 2-15 minutes of separations.
    Type: Grant
    Filed: November 9, 2007
    Date of Patent: April 24, 2012
    Assignee: Qiagen Sciences, LLC
    Inventors: Varouj Amirkhanian, Ming-Sun Liu, Andras Guttman
  • Publication number: 20120091005
    Abstract: Methods, systems, and compounds for detecting modified nucleic acid bases are disclosed and described. The methods provide for detecting a nucleic acid lesion and can include directing a nucleic acid adduct into a channel, wherein the nucleic acid adduct includes a nucleic acid having a lesion and a current modulating compound coupled to the nucleic acid at the lesion (110), and measuring a change in current through the channel in response to the current modulating compound to detect the lesion (112). The method can optionally include forming the nucleic acid adduct. Also provided is a method for identifying the number of repeat nucleotides in at least a portion of a nucleic acid strand, a method of assigning a registration marker within a nucleic acid, and a method of obtaining sequence information from a nucleic acid comprising assigning a registration marker on the nucleic acid.
    Type: Application
    Filed: September 7, 2011
    Publication date: April 19, 2012
    Inventors: Cynthia J. Burrows, Henry S. White, Ryuji Kawano, Aaron M. Fleming, Na An
  • Patent number: 8142630
    Abstract: Devices are provided for separating and focusing charged analytes, comprising a separation chamber and two or more electrodes, for example, an electrode array. A membrane separates the separation chamber and the electrodes. The separation chamber of the device is configured, that is, the separation chamber has a shaped geometry, which serves to induce a gradient in an electric field generated by the electrodes in the electrode chamber. Optionally, molecular sieve is included in the separation chamber that is operative to shift the location at which a stationary focused band of a charged analyte forms under a given set of focusing process parameters. Methods are provided for separating and focusing charged analytes comprising introducing a first fluid comprising at least one charged analyte into the separation chamber of a device as just described, applying an electric field gradient to the charged analyte to focus the charged analyte in the electric field gradient.
    Type: Grant
    Filed: May 19, 2004
    Date of Patent: March 27, 2012
    Assignees: Protasis Corporation, Washington State University
    Inventors: David Strand, Dan M. Leatzow, Cornelius F. Ivory
  • Patent number: 8137524
    Abstract: A device is disclosed for electrically controlled transport of ions between a source and a target electrolyte, including a source electrode and a target electrode. The electrodes are each capable of conducting ions, and the source electrode is arranged to receive ions from the source electrolyte and the target electrode is arranged to release ions to the target electrolyte. The device further includes an ion-conductive channel, arranged to receive ions from the source electrode and to release ions to the target electrode. Moreover, the ion-conductive channel is arranged to provide an ionic connection between the source and the target electrodes. The electrodes and the ion-conductive channel are formed of solid or semi-solid materials which are directly or indirectly attached to a support.
    Type: Grant
    Filed: June 4, 2007
    Date of Patent: March 20, 2012
    Assignee: Oboe IPR AB
    Inventors: Magnus Berggren, Peter Kjäll, David Nilsson, Nathaniel D. Robinson, Agneta Richter Dahlfors, Joakim Isaksson
  • Patent number: 8123925
    Abstract: An electrophoretic apparatus that allows bubbles to be readily removed out of an electrophoretic passage. The passage for electrophoretic medium, at a connecting section where capillaries filled with electrophoretic medium are connected with a pumping mechanism for filling the electrophoretic medium, is arranged such that the side of the pumping mechanism is disposed below the side of the capillaries, so that the electrophoretic medium flows from down to up at the connecting section when filling the electrophoretic medium into the capillaries. Preferably, the passage between the capillary array and the buffer solution is controlled by using a rotary-type valve having high withstand pressure to simplify the passage structure. The dead volume of the passage can be reduced and the valuable electrophoretic medium can be efficiently used. The amount of used electrophoretic medium required for the removal of the bubble can be also reduced.
    Type: Grant
    Filed: November 21, 2007
    Date of Patent: February 28, 2012
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Tomohiro Shoji, Jin Matsumura, Hidenori Namba
  • Publication number: 20120043208
    Abstract: A multi-channel gel electrophoresis apparatus for efficiently collecting molecules isolated by gel electrophoresis so they can be further analyzed, identified, or used as reagents or medications. The apparatus using a novel “tagless” strategy that combines multi-dimensional separation of endogenous complexes with mass spectrometric monitoring of their composition. In this procedure, putative protein complexes are identified based on the co-migration of collections of polypeptides through multiple orthogonal separation steps. A majority of E. coli proteins are shown to remain in stable complexes during fractionation of a crude extract through three chromatographic steps.
    Type: Application
    Filed: July 5, 2011
    Publication date: February 23, 2012
    Applicant: The Regents of the University of California
    Inventors: Jian Jin, Mark D. Biggin, Robert A. Nordmeyer, Ming Dong, Earl W. Cornell, Megan Choi, Halina Ewa Witkowski, Bong-Gyoon Han, Robert M. Glaeser
  • Publication number: 20120015355
    Abstract: A size standard, kit includes a size standard, method of defining a size standard and method of analysis using a size standard. The size standard is intended to include size standard elements which have a size greater than and/or less than and/or different from the components of a sample which are to be sized. This means that the same characteristic unit, such as a dye, can be used to label the component and the size standard. A further characteristic unit, from amongst a limited number of such characteristic units is liberated from use only on size standards for use on components. The method is therefore particularly useful in multiplex amplification of STRs.
    Type: Application
    Filed: June 29, 2009
    Publication date: January 19, 2012
    Applicant: FORENSIC SCIENCE SERVICE LIMITED
    Inventors: Valerie Carol Tucker, Pieris Koumi, Andrew Hopwood, Keith Elliot
  • Publication number: 20120000779
    Abstract: Provided herein is a surface acoustic wave (“SAW”) sensor device including an isolation component of a target biomolecule. A sample containing the target biomolecule is separated by its size using electrophoresis, and sequentially reacts with a SAW sensor. In other words, the device is capable of detecting the target biomolecule by separating biomolecules using electrophoresis, and applying the separated biomolecules to the SAW sensor.
    Type: Application
    Filed: December 21, 2010
    Publication date: January 5, 2012
    Applicants: AJOU UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION, SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Hun Joo LEE, Soo Suk LEE, Mi Jeong SONG, Kyung Yeon HAN, Jae Ho KIM
  • Publication number: 20120004434
    Abstract: The present invention provides germania-silica-based sol-gel monoliths that form the solid-phase material for extraction, isolation, preconcentration, and separation of analytes. In one embodiment, the germania-silica-based sol-gel monolith can be used to coat surfaces of the inner walls of extraction and chromatographic columns. In a preferred embodiment, the sol-gel germania-silica monolith of the present invention is formed from hydrolysis and polycondensation of precursors comprising tetraethoxygermane, tetramethoxysilane, and polyethylene glycol. Also provided are methods of preparing the germania-silica-based sol-gel monolith of the present invention.
    Type: Application
    Filed: June 3, 2011
    Publication date: January 5, 2012
    Applicant: University of South Florida
    Inventors: Abdul Malik, Erica B. Turner, Scott S. Segro
  • Publication number: 20110240471
    Abstract: The present invention provides exchangeable, reagent pre-loaded carriers (10), preferably in the form of plastic sheets, which can be temporarily applied to an electrode array (16) on a digital microfluidic (DMF) device (14). The carrier (10) facilitates virtually un-limited re-use of the DMF devices (14) avoiding cross-contamination on the electrode array (16) itself, as well as enabling rapid exchange of pre-loaded reagents (12) while bridging the world-to-chip interface of DMF devices (14). The present invention allows for the transformation of DMF into a versatile platform for lab-on-a-chip applications.
    Type: Application
    Filed: September 30, 2009
    Publication date: October 6, 2011
    Applicants: TECAN TRADING AG
    Inventors: Aaron R. Wheeler, Irena Barbulovic-Nad, Hao Yang, Mohamed Abdelgawad
  • Patent number: 8030092
    Abstract: An electrophoresis apparatus is generally disclosed for sequentially analyzing a single sample or multiple samples having one or more analytes in high or low concentrations. The apparatus comprises a relatively large-bore transport capillary which intersects with a plurality of small-bore separation capillaries and includes a valve system. Analyte concentrators, having antibody-specific (or related affinity) chemistries, are stationed at the respective intersections of the transport capillary and separation capillaries to bind one or more analytes of interest. The apparatus allows the performance of two or more dimensions for the optimal separation of analytes. The apparatus may also include a plurality of valves surrounding each of the analyte concentrators to localize each of the concentrators to improve the binding of one or more analytes of interest.
    Type: Grant
    Filed: November 17, 2006
    Date of Patent: October 4, 2011
    Assignee: Princeton Biochemicals, Inc.
    Inventor: Norberto A. Guzman
  • Patent number: 8007725
    Abstract: An electrophoresis apparatus is generally disclosed for sequentially analyzing a single sample or multiple samples having one or more analytes in high or low concentrations. The apparatus comprises a relatively large-bore transport capillary which intersects with a plurality of small-bore separation capillaries and includes a valve system. Analyte concentrators, having antibody-specific (or related affinity) chemistries, are stationed at the respective intersections of the transport capillary and separation capillaries to bind one or more analytes of interest. The apparatus allows the performance of two or more dimensions for the optimal separation of analytes. The apparatus may also include a plurality of valves surrounding each of the analyte concentrators to localize each of the concentrators to improve the binding of one or more analytes of interest.
    Type: Grant
    Filed: November 17, 2006
    Date of Patent: August 30, 2011
    Assignee: Princeton Biochemicals, Inc.
    Inventor: Norberto A. Guzman
  • Patent number: 7993507
    Abstract: The present invention is related to a method of separation of compounds by electrophoresis in which the compounds such as genes, proteins, etc. may be analyzed very precisely as samples are introduced directly into the separation tubes of the chip at the collection site, and therefore, it is not necessary to have separate fluid paths or individual sample storing apparatus that have been necessary for the conventional electrophoresis; it is easy to make the chips as the structure of the chip becomes extremely simple, and high-density arrangement of the separation tubes is enabled; and further, the compounds such as genes, proteins, etc. may be analyzed very precisely without interference in the storage tubs by using a non-polar solvent as the solvent of the sample storage tub.
    Type: Grant
    Filed: November 16, 2005
    Date of Patent: August 9, 2011
    Assignee: Korea Research Institute of Standards and Science
    Inventors: Sang-Ryoul Park, In-Chul Yang, Young-Ho Kim
  • Publication number: 20110186432
    Abstract: Various embodiments provide, for example, buffer compositions and/or sieving formulations useful in connection with electrophoresis instruments, such as capillary electrophoresis (CE) devices. In various embodiments, a buffer composition can include Bis-Tris, TAPS and/or TAPSO, and, optionally, a chelating agent, such as EDTA. Methods of separating samples containing bio-molecules, such as DNA or RNA, are also described.
    Type: Application
    Filed: January 28, 2011
    Publication date: August 4, 2011
    Applicant: APPLIED BIOSYSTEMS, LLC
    Inventors: Kevin J. Hacker, Karl O. Voss
  • Patent number: 7976691
    Abstract: A microfluidic device and method is disclosed having one or more membranes for the control of electrolysis. In one embodiment, a microfluidic device is disclosed that includes body with first channel and second channels separated by a gel layer. A first electrode positioned in the first channel and a second electrode positioned in the second channel wherein a potential applied to the first and second electrodes passes electrons from the first channel to the second channel through the gel layer. In another embodiment, a microfluidic device includes a body having a surface with a channel separating two first reservoirs. One or more membranes are positioned on the surface covering a portion of the channel and a blank is positioned covering the channel and the one or more membranes. A second reservoir through the blank is in contact with the membrane, each second reservoir in communication with the channel via the membrane.
    Type: Grant
    Filed: February 28, 2007
    Date of Patent: July 12, 2011
    Assignee: Intel Corporation
    Inventors: Scott Sibbett, Dimiter Petsev
  • Patent number: 7964077
    Abstract: The present invention is to provide a sample separation instrument used in a sample separation apparatus which includes: holding means for holding a first medium supporter which supports a first medium; and driving means for moving fixing means or the holding means in a direction parallel or perpendicular to a plane whose sides extend in the first direction and in the second directions. The sample separation instrument includes an insulator for storing a second medium which allows a sample separated in the first medium in the first direction to be further separated in the second direction different from the first direction, wherein: the insulator includes a first opening and a second opening each of which defines the second direction in which the second medium is electrified, and the second opening has a shape which allows the first medium containing the separated sample to be attached to the second medium along the first direction.
    Type: Grant
    Filed: August 28, 2006
    Date of Patent: June 21, 2011
    Assignees: Sharp Kabushiki Kaisha, Toppan Printing Co., Ltd
    Inventors: Yuji Maruo, Katsuyoshi Takahashi, Yutaka Unuma, Keisuke Usui, Hideki Kinoshita, Kisho Shiseki, Yoshio Suzuki, Atsunori Hiratsuka, Hiroyuki Fukui, Kenji Yokoyama, Ichiji Namatame, Kouhei Yodoya, Yasuhiro Ogawa, Koji Sakairi, Chie Hayashida, Satonari Akutsu, Kazuyoshi Yano, Isao Karube
  • Patent number: 7955575
    Abstract: A microfluidic device comprising a set of one or more, preferably more than 5, covered microchannel structures manufactured in the surface of a planar substrate. The device is characterized in that a part surface of at least one of the microchannel structures has a coat exposing a non-ionic hydrophilic polymer. The non-ionic hydrophilic polymer is preferably attached covalently directly to the part surface or to a polymer skeleton that is attached to the surface.
    Type: Grant
    Filed: December 11, 2000
    Date of Patent: June 7, 2011
    Assignee: Gyros Patent AB
    Inventors: Helene Derand, Anders Larsson, James Van Alstine
  • Patent number: 7862699
    Abstract: Matrix polymers and dynamic coating polymers, compositions thereof and related methods, systems and apparatus for microchannel separation.
    Type: Grant
    Filed: November 1, 2006
    Date of Patent: January 4, 2011
    Assignee: Northwestern University
    Inventors: Annelise E. Barron, Cheuk Wai Kan, Christopher P. Fredlake
  • Patent number: 7828949
    Abstract: Provided are a biomolecule detection device, a mobile phone for biomolecule detection, and a biomolecule detection method. The biomolecule detection device includes an electrophoresis unit comprising an electrophoretic gel filtering erythrocytes and leukocytes in blood and transferring proteins and DNAs in the blood, and at least one type of a probe biomolecule, immobilized in the electrophoretic gel, reacting with a target biomolecule; a conversion unit converting a result of a reaction between the target biomolecule and the probe biomolecule to an electrical signal; and a lead-out unit receiving, converting, and transmitting the electrical signal.
    Type: Grant
    Filed: December 6, 2006
    Date of Patent: November 9, 2010
    Assignee: Electronics and Telecommunications Research Institute
    Inventors: Moon Youn Jung, Se Ho Park, Dong Ho Shin, Hyeon Bong Pyo
  • Patent number: RE43122
    Abstract: The present invention provides an on-chip packed reactor bed design that allows for an effective exchange of packing materials such as beads at a miniaturized level. The present invention extends the function of microfluidic analysis systems to new applications including on-chip solid phase extraction (SPE) and on-chip capillary electrochromatography (CEC). The design can be further extended to include integrated packed bed immuno- or enzyme reactors.
    Type: Grant
    Filed: June 22, 2010
    Date of Patent: January 24, 2012
    Assignee: The Governors of the University of Alberta
    Inventors: D. Jed Harrison, Richard Oleschuk, Loranelle L. Lockyear, Cameron Skinner, Paul Li