With Detailed Detection System (e.g., Including A Light Source And A Camera, Etc.) Patents (Class 204/612)
  • Patent number: 9552529
    Abstract: A method and system to enhance analysis of electrophoretic bands by overlaying only the pixels of interest. The overlaid pixels are superimposed as a layer above, i.e., in the foreground of, the overlaid image, i.e., in the background. A user employs the superimposed pixels for molecular weight determination and is still able to generate densitometry analysis of the remaining pixels in the overlaid image.
    Type: Grant
    Filed: December 1, 2015
    Date of Patent: January 24, 2017
    Assignee: PIERCE BIOTECHNOLOGY, INC.
    Inventors: Rachael Mae Berry, Nikki L. Jarrett, Eric Leigh Hommema, Kelli D. Feather-Henigan, Suk J. Hong, Brian Lynn Webb
  • Patent number: 9230185
    Abstract: A method and system to enhance analysis of electrophoretic bands by overlaying only the pixels of interest. The overlaid pixels are superimposed as a layer above, i.e., in the foreground of, the overlaid image, i.e., in the background. A user employs the superimposed pixels for molecular weight determination and is still able to generate densitometry analysis of the remaining pixels in the overlaid image.
    Type: Grant
    Filed: March 6, 2013
    Date of Patent: January 5, 2016
    Assignee: Pierce Biotechnology, Inc.
    Inventors: Rachael Mae Berry, Nikki L. Jarrett, Eric Leigh Hommema, Kelli D. Feather-Henigan, Suk J. Hong, Brian Lynn Webb
  • Patent number: 9228948
    Abstract: Devices, systems, kits, and methods for detecting and/or identifying a plurality of spectrally labeled bodies well-suited for performing multiplexed assays. By spectrally labeling the beads with materials which generate identifiable spectra, a plurality of beads may be identified within the fluid. Reading of the beads is facilitated by restraining the beads in arrays, and/or using a focused laser.
    Type: Grant
    Filed: April 30, 2015
    Date of Patent: January 5, 2016
    Assignee: Life Technologies Corporation
    Inventors: Stephen Empedocles, Andrew Watson, Jian Jin
  • Patent number: 9151701
    Abstract: The systems and methods disclosed herein include a microfluidic system, comprising a pneumatic manifold having a plurality of apertures, and a chip manifold having channels disposed therein for routing pneumatic signals from respective ones of the apertures to a plurality of valves in a microfluidic chip, wherein the channels route the pneumatic signals in accordance with a configuration of the plurality of valves in the microfluidic chip.
    Type: Grant
    Filed: December 11, 2013
    Date of Patent: October 6, 2015
    Assignee: RHEONIX, INC.
    Inventors: Peng Zhou, Lincoln C. Young
  • Publication number: 20150083594
    Abstract: An electrophoresis controller for use with an electrophoresis apparatus having a gel matrix disposed between electrodes for separation of particles along with a tracking dye. The electrophoresis controller includes a sensor system and a controller. The sensor system includes a support, a light emitter, and a light receiver. The support includes a first portion positionable on a first side of the gel matrix and a second portion positionable adjacent a second side of the gel matrix. The light emitter is positioned on the first portion of the support for emitting light onto one side of the gel matrix. The light receiver is positioned on the second portion of the support adjacent to the other side of the gel matrix for receiving light from the light source as it is passing through the gel matrix. At least one of the light emitter and the light receiver includes a light guide having a first end and a second end.
    Type: Application
    Filed: October 22, 2014
    Publication date: March 26, 2015
    Applicant: THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK
    Inventors: Papa Nii ASARE-OKAI, Rabi A. MUSAH, Tony P. HOANG
  • Patent number: 8974651
    Abstract: The present invention discloses apparatuses and methods for the visualization of fluorophores in biological systems such as electrophoresis gels and cell cultures. Preferred embodiments of the apparatuses consists of a tray or tank having one or more light sources disposed to direct light through an electrophoresis gel disposed within the vessel such that the luminescence from fluorophores in the gel are easily visualized.
    Type: Grant
    Filed: April 17, 2011
    Date of Patent: March 10, 2015
    Assignee: C.C. IMEX
    Inventors: Richard Chan, Winston Glenn Walker, Rita M. Wong, Jason In
  • Publication number: 20140332389
    Abstract: A kit for separating and concentrating nucleic acid and protein targets includes labeled reagents which affect simultaneous co-purification and concentration of a nucleic acid and a protein, a gel isotachophoresis separation unit to which a sample comprising the nucleic acid and the protein is added, a detection unit for the detection of the presence of the nucleic acid and the protein, and instructions for use. The gel electrophoresis includes a gel box having a negative electrode side and a positive electrode side, the negative electrode side being filled with a first buffer comprising 2-Hydroxy-N-(tris(hydroxymethyl)methyl)-3-aminopropanesulfonic acid buffer and the positive electrode side being filled with a second buffer being different than the first buffer. The gel isotachophoresis separation is configured to subject the sample to isotachophoresis using a voltage.
    Type: Application
    Filed: July 30, 2014
    Publication date: November 13, 2014
    Inventors: Charles C. Young, Alex J. Proescher, Emily E. Smith
  • Publication number: 20140158541
    Abstract: Cassette electrophoresis systems that allow viewing of molecules during the electrophoresis run are disclosed. Cassette electrophoresis bases that reversibly engage light sources, such as light source bases are disclosed. Also disclosed are visible light transillumination systems for viewing a pattern of fluorescence emitted by fluorophores comprising a cassette housing fluorophore-containing material and a base unit to support the cassette. In some aspects the base unit that includes a power supply also houses a light source having output in the visible wavelength region and a filter placed between the light source and the fluorophores. The system is constructed and arranged such that patterns of fluorescence emitted by the fluorophores are viewable.
    Type: Application
    Filed: November 4, 2013
    Publication date: June 12, 2014
    Applicant: LIFE TECHNOLOGIES ISRAEL LTD.
    Inventors: Matthew P. Beaudet, Ilana Margalit
  • Patent number: 8652316
    Abstract: An electrophoresis system includes a pedestal including a base, two enlargements at both ends of the base respectively, two cavities in the enlargements respectively, and a transparent plate on top of the base; a photographic filter member provided on the top of the base; a control panel on one enlargement; an electrophoresis tank provided between the enlargements; a covering member releasably provided on the pedestal for concealing the electrophoresis tank; a gel block in the electrophoresis tank for holding DNA samples in place; LED lamps in each cavity, the LED lamps being for emitting blue light toward the DNA samples; a hollow mount on the covering member and including a sliding tray; a photographic filter releasably disposed on the tray; and a picture taking device mounted on the hollow mount.
    Type: Grant
    Filed: September 24, 2012
    Date of Patent: February 18, 2014
    Assignee: Major Science Co., Ltd.
    Inventors: Wen-Liang Chuu, Chian-Fu Wang
  • Publication number: 20130199930
    Abstract: Disclosed in this specification is a high capacity compact gel documentation system for documenting different types of electrophoresis gels or other translucent objects using ultraviolet light. The system includes a base with a scanning surface having a transparent bottom surface. A light source is connected to a conveying mechanism, disposed below the transparent bottom surface, to move the light source over the length of the transparent bottom surface. An image capture device receives a reflected image and provides it to a microprocessor. Moreover, separate interchangeable filters allow for documentation of UV and white light gel captures, alleviating the need for separate transilluminators and hoods with filters and cameras.
    Type: Application
    Filed: February 7, 2013
    Publication date: August 8, 2013
    Applicant: Research Foundation of The City University of New York
    Inventor: Research Foundation of The City University of New York
  • Publication number: 20130175172
    Abstract: The present teachings provide methods, devices, systems, and materials for performing electrophoresis in an automated fashion. The electrophoresis system may simultaneously image gel during an electrophoresis run. In some embodiments, the electrophoresis system may analyze an imaged gel during or after electrophoresis. The device may comprise a gel processing system, a gel illumination system, an image capture system, and an image analysis all housed within a housing.
    Type: Application
    Filed: December 17, 2012
    Publication date: July 11, 2013
    Applicant: Life Technologies Corporation
    Inventor: Life Technologies Corporation
  • Publication number: 20130140183
    Abstract: The disclosed device for trapping biologically-relevant substances can easily collect biologically-relevant substances from a thin tissue section or a gel in which said biologically-relevant substance have been fractionated. Said device is provided with: trapping bodies for trapping biologically-relevant substances; carriers that hold the trapping bodies; and electrodes for charging the trapping bodies. The trapping bodies are charged and brought into contact with a prescribed position on a thin tissue section that contains biologically-relevant substances or a gel in which biologically-relevant substances have been fractionated, thereby trapping said biologically-relevant substances from said gel or thin tissue section.
    Type: Application
    Filed: June 22, 2011
    Publication date: June 6, 2013
    Inventor: Hideji Tajima
  • Publication number: 20130112562
    Abstract: This invention avoids the need for UV light as well as all the various filters utilized by prior systems while allowing real-time observation and recording of molecules during electophoresis. This invention is a real-time observable electrophoresis system that avoids the use of light filters as the activation of fluorescent dyes is done by LEDs that give off visible light with a certain wavelength, instead of ultraviolet light, which are positioned not on the top or bottom of the electrophoresis gel but on the sides. The background light that is observed in some gels is eliminated by the image analyzing computer software. This system eliminates the need to filter the wavelength of the activating light and enables the observation and recording of the molecules during the entirety of the electrophoresis.
    Type: Application
    Filed: November 4, 2011
    Publication date: May 9, 2013
    Applicant: SALUBRIS, INC.
    Inventor: Tanil Zuhtu Kocagoz
  • Publication number: 20130075261
    Abstract: The present embodiments provide systems, kits and methods suitable for performing dry or substantially dry electro-blotting analyses on immobilized protein or nucleic acid samples. Electro-blotting performed according to the presently described embodiments may include a step whereby detection of one or more immobilized proteins or nucleic acids is electrophoretically accelerated. Methods for performing electro-blotting of immobilized proteins or nucleic acids may include applying an electric voltage to one or more reagents typically used in protein or nucleic acid blotting procedure. The one or more reagents may be absorbed on a suitable carrier matrix. Electro-blotting performed in accordance with the systems and methods described herein may be performed under substantially dry conditions (i.e., with little or no aqueous buffers).
    Type: Application
    Filed: September 12, 2012
    Publication date: March 28, 2013
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Ilana MARGALIT, Galia Feldman, Svetlana Lifshits
  • Patent number: 8241475
    Abstract: A method is provided for separating single- and double-stranded nucleic acid molecules based on their strandness and length.
    Type: Grant
    Filed: September 2, 2005
    Date of Patent: August 14, 2012
    Assignee: Lifeind ehf.
    Inventors: Gudmundur H. Gunnarsson, Hans Guttormur Thormar, Bjarki Gudmundsson, Jon Johannes Jonsson
  • Publication number: 20110308954
    Abstract: A gel excitation apparatus for exciting a gel that includes a container for holding an aqueous solution within which the gel can be positioned, at least one light source disposed whereas the light path is perpendicular to the optical axis, the container for irradiating the gel with radiation within at least a first wavelength band, the first wavelength band selected to excite the gel to cause the gel to emit radiation into said aqueous solution. The aqueous core of the apparatus has a low refractive index, thus retaining the first wavelength band selected within said aqueous core to excite the gel. Thus, the aqueous core functions not only to increase the efficiency of excitation, but also increases the contrast between excitation light and sample emission light by retaining the light within said aqueous core.
    Type: Application
    Filed: August 31, 2011
    Publication date: December 22, 2011
    Inventors: Darius Kelly, Sean Gallagher
  • Publication number: 20110300552
    Abstract: The present invention is based on the discovery that drug resistance in microorganisms can be rapidly and accurately determined using mass spectrometry. A mass spectrum of an intact microorganism or one or more isolated biomarkers from the microorganism grown in drug containing, isotopically-labeled media is compared with a mass spectrum of the intact microorganism or one or more isolated biomarkers from the microorganism grown in non-labeled media without the drug present. Drug resistance is determined by predicting and detecting a characteristic mass shift of one or more biomarkers using algorithms. The characteristic mass shift is indicative that the microorganism is growing in the presence of the drug and incorporating the isotopic label into the one or more biomarkers, resulting in change in mass.
    Type: Application
    Filed: February 17, 2011
    Publication date: December 8, 2011
    Inventors: Plamen A. Demirev, Nathan A. Hagan, Miquel D. Antoine, Jeffrey S. Lin, Andrew B. Feldman
  • Publication number: 20110259744
    Abstract: A sensor device is provided for detecting an analyte in a sample in which an analyte is bound to a detection reagent to form a bound complex. The device comprises (a) a sample (5) comprising an ionic analyte and a detection reagent in a conductive fluid, wherein the detection reagent has a net charge different from the analyte; (b) a first permeable polymeric hydrogel plate (3) and a first spacer plate (8), which plates provide a compartment for the sample; (c) an anode (1) juxtaposed to the outside of the first hydrogel plate and not in contact with the sample; (d) a cathode (9) juxtaposed to the outside of the first spacer plate and not in contact with the sample; (e) a voltage generator (10) to apply an electric potential to the anode and cathode; and (f) a detector (11).
    Type: Application
    Filed: May 18, 2010
    Publication date: October 27, 2011
    Inventor: William R. Moyle
  • Publication number: 20110253541
    Abstract: The present invention discloses apparatuses and methods for the visualization of fluorophores in biological systems such as electrophoresis gels and cell cultures. Preferred embodiments of the apparatuses consists of a tray or tank having one or more light sources disposed to direct light through an electrophoresis gel disposed within the vessel such that the luminescence from fluorophores in the gel are easily visualized.
    Type: Application
    Filed: April 17, 2011
    Publication date: October 20, 2011
    Inventors: Richard Chan, Winston Glenn Walker, Rita M. Wong, Jason In
  • Patent number: 8018593
    Abstract: The present disclosure provides fully integrated microfluidic systems to perform nucleic acid analysis. These processes include sample collection, nucleic acid extraction and purification, amplification, sequencing, and separation and detection. The present disclosure also provides optical detection systems and methods for separation and detection of biological molecules. In particular, the various aspects of the invention enable the simultaneous separation and detection of a plurality of biological molecules, typically fluorescent dye-labeled nucleic acids, within one or a plurality of microfluidic chambers or channels. The nucleic acids can be labeled with at least 6 dyes, each having a unique peak emission wavelength. The present systems and methods are particularly useful for DNA fragment sizing applications such as human identification by genetic fingerprinting and DNA sequencing applications such as clinical diagnostics.
    Type: Grant
    Filed: April 4, 2008
    Date of Patent: September 13, 2011
    Assignee: NetBio, Inc.
    Inventors: Eugene Tan, Heung Chuan Lam, Valery Leonidovich Bogdanov, Gregory John Kellogg, John A. Wright, Ulrich Hans Thomann, Richard F. Selden
  • Patent number: 7967968
    Abstract: A method and system are presented for use in analyzing a sample. The method comprises applying real time monitoring to a sample while undergoing a separation process consisting of spatial separation of molecules of different molecular weights in the sample. The system includes a monitoring unit configured to be integrated with a separation unit in which the separation process takes place.
    Type: Grant
    Filed: March 15, 2005
    Date of Patent: June 28, 2011
    Assignee: D.N.R.-Imaging Systems Ltd.
    Inventors: Yoran Kober, Meir Matana, Erez Kelly
  • Publication number: 20110147218
    Abstract: The invention relates to a method for the diagnosis and risk stratification of a tumor or cancer of the gastrointestinal tract, particularly colon tumor or colon cancer, wherein a determination is made on at least one patient using the novel soluble cadherin 17 biomarker—a proteolytic cleavage product of cadherin 17 having SEQ ID No. 1 or SEQ ID No. 2—or partial peptides and fragments thereof.
    Type: Application
    Filed: July 28, 2008
    Publication date: June 23, 2011
    Inventors: Helmut Meyer, Hanna Diehl, Susanne Klein-Scory, Wolff Schmiegel, Irmgard Schwarte-Waldhoff, Kai Stühler, Jakob Weiss
  • Patent number: 7943027
    Abstract: The object of the present invention is that a dynamic range is extended in an electrophoresis unit and concentration differences among a plurality of samples measured simultaneously are increased. An irradiation time to the samples is adjusted during analysis without changing a sampling time. By shortening the irradiation time, a fluorescence amount of the samples is reduced to cause signal intensity detected by a detector to physically decrease. If the irradiation time is very short (several 100 msec), the irradiation time and fluorescence intensity are in a direct proportional relationship. It is known that, if the irradiation time is reduced to 1/n, the fluorescence intensity, that is, signal intensity to be detected will be 1/n. Thus, for data whose irradiation time is reduced to 1/n during analysis, data obtained by multiplying a substantially measured value by n is used for data analysis as a true value to be originally acquired.
    Type: Grant
    Filed: November 6, 2007
    Date of Patent: May 17, 2011
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Motohiro Yamazaki, Ryoji Inaba, Satoshi Takahashi, Tomohiro Shoji, Takeshi Ohura, Takashi Gomi
  • Publication number: 20110094886
    Abstract: A concept of high resolution electrophoretic technique, apparatus and its application thereof. The system is a functioning entity in a radial-flat setting. Unlike the conventional rectangular gel-electrophoresis setup, wherein two electrically opposite poles are placed on opposite sites of a rectangular gel, the novel system places one of the electrical poles in the middle of the gel and the outer rims of the radial gel is exposed to the other pole. The point, central pole (positive/negative) and the radial (negative/positive) pole around the outer rim of the gel will create and maintain a gradient electric field, wherein the intensity of the electric field increases towards the central pole with an inverse proportionally to the decrease in the surface area towards the center in a radial-gradient setting.
    Type: Application
    Filed: May 5, 2008
    Publication date: April 28, 2011
    Inventor: Celal Korkut Vata
  • Patent number: 7931791
    Abstract: The invention provides methods for detecting an interaction between an analyte and a biomolecule. The method comprises separating at least one biomolecule according to its isoelectric point in the presence of a given analyte and detecting an interaction between the analyte and a biomolecule using fluorescence anisotropy. The method may further comprise collecting the analyte-biomolecule complex and analyzing the biomolecule.
    Type: Grant
    Filed: October 24, 2007
    Date of Patent: April 26, 2011
    Assignee: Southern Illinois University Carbondale
    Inventors: Luke Tolley, Matt McCarroll
  • Publication number: 20110062024
    Abstract: The invention provides an electrophoresis cassette, methods for making the electrophoresis cassette, and method of fractionating analytes from a sample based upon electrophoretic mobility in a single application of the sample to an electrophoretic system.
    Type: Application
    Filed: April 14, 2010
    Publication date: March 17, 2011
    Inventors: Douglas Grosvenor Sabin, Todd J. Barbera, Paul Chandler Sabin, T. Christian Boles
  • Publication number: 20110042213
    Abstract: The present teachings provide methods, devices, systems, and materials for performing electrophoresis in an automated fashion. The electrophoresis system may simultaneously image gel during an electrophoresis run. In some embodiments, the electrophoresis system may analyze an imaged gel during or after electrophoresis. The device may comprise a gel processing system, a gel illumination system, an image capture system, and an image analysis all housed within a housing.
    Type: Application
    Filed: August 24, 2010
    Publication date: February 24, 2011
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Timothy UPDYKE, Paul PREDKI, Evangeline GONZALEZ, Randall LOWE, Tad SIMONS
  • Patent number: 7875162
    Abstract: A device for separating and purifying useful quantities of particles comprises: a. an anolyte reservoir connected to an anode, the anolyte reservoir containing an electrophoresis buffer; b. a catholyte reservoir connected to a cathode, the catholyte reservoir also containing the electrophoresis buffer; c. a power supply connected to the anode and to the cathode; d. a column having a first end inserted into the anolyte reservoir, a second end inserted into the catholyte reservoir, and containing a separation medium; e. a light source; f. a first optical fiber having a first fiber end inserted into the separation medium, and having a second fiber end connected to the light source; g. a photo detector; h. a second optical fiber having a third fiber end inserted into the separation medium, and having a fourth fiber end connected to the photo detector; and i. an ion-exchange membrane in the anolyte reservoir.
    Type: Grant
    Filed: March 28, 2005
    Date of Patent: January 25, 2011
    Assignee: Board of Regents, University of Houston
    Inventors: George William Jackson, Richard Coale Willson, George Edward Fox
  • Patent number: 7854827
    Abstract: A device providing an arrangement for the separation of a sample mixture for analytical reasons based on multidimensional gel electrophoresis and method thereof are disclosed. The separation involves a first separation in a first dimension of the device on the basis of isoelectric points and a second separation in a second dimension of the device on the basis of molecular size. At least two gel strips for the first dimension separation step and a corresponding gel for the second dimension separation step are provided. The two gel strips are arranged on a single carrier either on the same side or on opposite sides. By having at least two gel strips arranged in such a manner at least two analytical processes can be executed in parallel on the single carrier without the use of valves.
    Type: Grant
    Filed: December 20, 2006
    Date of Patent: December 21, 2010
    Assignee: Roche Diagnostics Operations, Inc.
    Inventor: Mario Curcio
  • Publication number: 20100294664
    Abstract: The invention relates to an assay for discriminating between amyotrophic lateral sclerosis (ALS) patients and patients with ALS-like disorders that express symptoms like ALS. The method is based on the use of 2-dimensional (2D) gel electrophoresis to separate the complex mixture of proteins found in blood serum, the quantitation of a group of identified biomarkers, and the biostatistical analysis of the concentration of the identified biomarkers to differentiate patients having ALS from patients having other ALS-like disorders.
    Type: Application
    Filed: July 30, 2010
    Publication date: November 25, 2010
    Inventors: Ira Leonard Goldknopf, Essam Ahmed Sheta, Brian R. Folsom, Stanley H. Appel, Albert A. Yen, Erica P. Simpson
  • Publication number: 20100288638
    Abstract: A method and apparatus for performing separation of molecules, in particular biomolecules such as nucleic acids and peptides, are disclosed. The method comprises separating molecules by a first characteristic along a linear dimension of a channel, and separating the molecules by a second characteristic along the same linear dimension, with the rust and second separations being carried out within at least partially overlapping sections of the channel. The two separations may then be combined to give a virtual two-dimensional separation which is carried out in a single dimensional real space. The method may also include detecting molecules during the second separation, preferably using an intrinsic characteristic of the molecules for example UV absorbance.
    Type: Application
    Filed: March 29, 2007
    Publication date: November 18, 2010
    Applicant: DELTADOT LIMITED
    Inventor: Stuart Hassard
  • Patent number: 7828949
    Abstract: Provided are a biomolecule detection device, a mobile phone for biomolecule detection, and a biomolecule detection method. The biomolecule detection device includes an electrophoresis unit comprising an electrophoretic gel filtering erythrocytes and leukocytes in blood and transferring proteins and DNAs in the blood, and at least one type of a probe biomolecule, immobilized in the electrophoretic gel, reacting with a target biomolecule; a conversion unit converting a result of a reaction between the target biomolecule and the probe biomolecule to an electrical signal; and a lead-out unit receiving, converting, and transmitting the electrical signal.
    Type: Grant
    Filed: December 6, 2006
    Date of Patent: November 9, 2010
    Assignee: Electronics and Telecommunications Research Institute
    Inventors: Moon Youn Jung, Se Ho Park, Dong Ho Shin, Hyeon Bong Pyo
  • Publication number: 20100190192
    Abstract: The present invention relates to a marker for the development of pre-eclampsia. In particular the invention provides a marker for the development of pre-eclampsia, which marker consists of a polypeptide of approximately 26.6 Kd as determined by 15-30% gradient SDS-PAGE under reducing conditions.
    Type: Application
    Filed: September 15, 2006
    Publication date: July 29, 2010
    Inventor: Victor Voroteliak
  • Publication number: 20100140090
    Abstract: A method and apparatus for expeditiously carrying out the preparation and imaging steps of the electrophoresis process. The apparatus of the invention includes a novel supporting surface comprising TEFLON® AF that acts as a multi-function gel supporting surface which minimizes the probability of damaging the gel during transfer and provides a supporting surface that has an index of refraction lower than that of water and that of water based gel. When the TEFLON® AF supporting surface is used to support the gel, or other mostly aqueous, fluorescent sample, the surface becomes a planar, aqueous core waveguide which traps the excitation light thereby increasing the efficiency of excitation.
    Type: Application
    Filed: December 9, 2008
    Publication date: June 10, 2010
    Inventors: Darius Kelly, Sean Gallagher
  • Publication number: 20100140094
    Abstract: A gel excitation apparatus for exciting a gel that includes a container for holding an aqueous solution within which the gel can be positioned, at least one light source disposed proximate the container for irradiating the gel with radiation within at least a first wavelength band, the first wavelength band selected to excite the gel to cause the gel to emit radiation into said aqueous solution. The aqueous core of the apparatus functions not only to increase the efficiency of excitation, but also increases the contrast between excitation light and sample emission light.
    Type: Application
    Filed: December 9, 2008
    Publication date: June 10, 2010
    Inventors: Darius Kelly, Sean Gallagher
  • Publication number: 20100126862
    Abstract: The invention provides an electrophoresis cassette, methods for making the electrophoresis cassette, and method of fractionating analytes from a sample based upon electrophoretic mobility in a single application of the sample to an electrophoretic system.
    Type: Application
    Filed: October 8, 2009
    Publication date: May 27, 2010
    Applicant: Sage Science, Inc.
    Inventors: Douglas G. Sabin, Todd J. Barbera, Paul Chandler Sabin, T. Christian Boles
  • Publication number: 20100084270
    Abstract: The present invention relates to a method for purifying analyte molecules and in particular to a component of this type in which a separation section is used for separating analyte molecules and other constituents of a sample, and in which provision is made of at least one sample chamber for receiving a sample containing the analyte molecules and at least one collecting chamber for receiving the purified analyte molecules. According to the invention, the microfluidic component has at least one integrated receptor device for detecting the presence and/or the concentration of the purified analyte molecules. In accordance with one advantageous development of the present invention, the separation section is formed by an electrophoretic gel filtration section.
    Type: Application
    Filed: October 26, 2007
    Publication date: April 8, 2010
    Applicant: ALBERT-LUDWIGS-UNIVERSITAT FREIBURG
    Inventors: Paul Vulto, Gerald Urban
  • Patent number: 7692162
    Abstract: Images of two-dimensional chromatograms or other sample arrays are formed on a scanning instrument that utilizes a line of illumination light that sweeps the length of the array either by moving across the array or by the array moving relative to the light, in either case scanning the entire two-dimensional array with a unidirectional pass of the moving component. The use of a CCD equipped with time delay integration allows the instrument to form an enhanced image.
    Type: Grant
    Filed: December 13, 2007
    Date of Patent: April 6, 2010
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Suresh N. Mehta, Neeraj Bhatt, Kevin A. McDonald
  • Publication number: 20100034445
    Abstract: The present invention relates to devices, systems, and methods for continuous imaging of nucleic acids. In one embodiment, the invention herein generally relates to a device for continuously imaging nucleic acids, the device including a rotating drum in which an exterior surface of the drum includes a plurality of color sensitive pixels.
    Type: Application
    Filed: August 6, 2008
    Publication date: February 11, 2010
    Applicant: Genome Corporation
    Inventor: Kevin Ulmer
  • Publication number: 20100035228
    Abstract: A device for separating and purifying useful quantities of particles comprises: a. an anolyte reservoir connected to an anode, the anolyte reservoir containing an electrophoresis buffer; b. a catholyte reservoir connected to a cathode, the catholyte reservoir also containing the electrophoresis buffer; c. a power supply connected to the anode and to the cathode; d. a column having a first end inserted into the anolyte reservoir, a second end inserted into the catholyte reservoir, and containing a separation medium; e. a light source; f. a first optical fiber having a first fiber end inserted into the separation medium, and having a second fiber end connected to the light source; g. a photo detector; h. a second optical fiber having a third fiber end inserted into the separation medium, and having a fourth fiber end connected to the photo detector; and i. an ion-exchange membrane in the anolyte reservoir.
    Type: Application
    Filed: March 28, 2005
    Publication date: February 11, 2010
    Applicant: Board of Regents, University of Houston
    Inventors: George William Jackson, Richard Coale Willson, George Edward Fox
  • Patent number: 7622027
    Abstract: The invention provides methods of detecting, purifying, quantifying, and separating molecules using an electrophoresis adapted biosensor.
    Type: Grant
    Filed: August 29, 2005
    Date of Patent: November 24, 2009
    Assignee: SRU Biosystems, Inc.
    Inventor: Brian T. Cunningham
  • Publication number: 20090277791
    Abstract: A method for identifying and separating of target biomolecules is disclosed. The method involves contacting a sample with a nanoparticle probe that is capable of specifically binding a target biomolecule. The complex formed by the nanoparticle target biomolecule is separated by electrophoresis using a non-conventional gel composed of a synthetically cross-linked polymer and a gel stabilizer. The nanoparticle probe:target biomolecule complex is detected, thereby identifying the target biomolecule. In certain examples, the nanoparticle probe emits a signal to identify its location. Particular examples of nanoparticle probes are semiconductor nanocrystals, such as quantum dots, which fluoresce at a particular wavelength. Also disclosed is a method of substantially sealing and/or increasing the transparency of a membrane. Such a method includes applying a film to a membrane. Sealing the membrane allows the detection of single nanoparticles.
    Type: Application
    Filed: October 23, 2007
    Publication date: November 12, 2009
    Inventors: Tania Q. Vu, Hong Yan Liu
  • Publication number: 20090211908
    Abstract: Devices and methods are described for detecting and quantifying nucleic acids using a sealed system that minimizes contamination. In particular, provided herein are devices for and methods using nucleic acid amplification that permit multiple sampling of an amplification reaction mixture and quantitation and identification of amplicons during the course of an amplification reaction. Methods involving the transfer of samples from an amplification reaction mixture into a separation network, separation of nucleic acids based on size, and identification and quantitation of nucleic acids are disclosed.
    Type: Application
    Filed: January 22, 2009
    Publication date: August 27, 2009
    Inventor: Javier Farinas
  • Publication number: 20090071829
    Abstract: Provided herein are biological research methods, kits, and products that utilize radio frequency identifier technology.
    Type: Application
    Filed: July 28, 2008
    Publication date: March 19, 2009
    Applicant: INVITROGEN CORPORATION
    Inventors: Matthew O'Banion, Emanuel J. Vacchiano, Traci Libby, Charles Piazza
  • Patent number: 7497934
    Abstract: An analysis system for and method of enabling determination of the velocities of migrating objects and also classifying migrating objects into groups having a common constraint. In one aspect the present invention provides an analysis system for and method of enabling determination of the velocities of migrating objects, the system comprising: a space-time map generator for generating a space-time map of points representative of the signal peaks of signals detected at a plurality of spaced positions; and a vertex finder for identifying at least one vertex from the space-time map, with a single vertex being identified for each group of objects having a common constraint and the velocities of the objects being determinable from the sets of points in the space-time map corresponding to the respective objects as fitted to the respective vertex.
    Type: Grant
    Filed: July 20, 2001
    Date of Patent: March 3, 2009
    Assignee: Deltadot Ltd.
    Inventor: Dimitrios Sideris
  • Publication number: 20080257737
    Abstract: Disclosed is a technique for electrophoresis analysis, capable of eliminating the need for labeling a sample with a fluorescent or radioactive material. An electrophoresis cell is formed with a plurality of electrophoretic paths arranged in parallel relation to each other. Each of the electrophoretic paths has an upper end serving as a sample injection end, and a lower end received in a sample receiver. Further, each of the electrophoretic paths has a plurality of microchannels each extending uniformly from a position apart from the upper end by a predetermined distance to the lower end. The microchannels serve as a diffraction grating for diffracting light which irradiates a surface of the electrophoresis cell in a direction perpendicular to the surface, to produce diffracted light.
    Type: Application
    Filed: September 28, 2007
    Publication date: October 23, 2008
    Applicant: SHIMADZU CORPORATION
    Inventor: Yukihisa WADA
  • Patent number: 7384527
    Abstract: The invention provides an electrophoresis apparatus comprising an electrophoretic chamber containing at least on two-plate housing for a sieving matrix, each said at least one two-plate housing consisting of a cover plate and a plate for housing said sieving matrix, wherein said plate includes an optical waveguide array which is characterized by being aligned with respect to a reference direction such as to enable one or more light beams incident on the sieving matrix along said reference direction to be maintained well-confined along said reference direction within any desired length of interaction along the width of said sieving matrix.
    Type: Grant
    Filed: August 1, 2001
    Date of Patent: June 10, 2008
    Assignee: Sensis Ltd
    Inventors: Doron Goldberg, Zeev Weissman, Menachem Shapiro, Shlomo Ruschin
  • Patent number: 7309409
    Abstract: A bio-separation system using an efficient, compact, portable, interchangeable, reusable, recyclable, multi-channel cartridge, has integrated pre-aligned optics and an integrated reagent reservoir. The cartridge supports, for example, multiple capillaries for CE separation. An integrated reservoir containing a separation support medium (e.g., a gel buffer) is common to all capillaries. The chemistry of the medium and the characteristics of the capillaries (e.g., capillary size, coating and length) are defined for each cartridge. Different cartridges can be easily interchanged in the bio-separation system to suit the particular sample based separation. The reservoir is coupled to an air pressure pump that pressurizes the gel reservoir to purge and fill the capillaries with buffer as the separation support medium.
    Type: Grant
    Filed: January 28, 2002
    Date of Patent: December 18, 2007
    Assignee: Biocal Technology, Inc.
    Inventors: Varouj Amirkhanian, Ming-Sun Liu, Paul Mooney
  • Patent number: 7217352
    Abstract: An electrophoresis device is disclosed that is capable of reducing waves occurring in a fluid in which the capillary tips of a capillary assay are immersed. A plurality of partition plate members can be provided in a container containing the fluid to divide the surface of the fluid into several sections. The container can be transported at a high speed, and the capillary tips can be immersed in the fluid quickly, thereby preventing resolution degradation caused by a long period of exposure of the capillary tips to air. Throughput of sample analysis can also be improved because it is not necessary to wait until waves disappear before conducting an analysis.
    Type: Grant
    Filed: May 28, 2003
    Date of Patent: May 15, 2007
    Assignees: Hitachi High-Technologies Corporation, Applera Corporation
    Inventors: Taisaku Seino, Tomoyuki Sakai, Miho Ozawa, Masaya Kojima, Eric S. Nordman
  • Patent number: 7208072
    Abstract: A bio-separation instrument using a multi-segment cartridge. The cartridge includes a plurality of efficient, compact, portable, interchangeable, reusable, recyclable, modular, multi-channel segments. Each segment supports multiple capillaries for CE separation. Each segment has an integrated reservoir containing a buffer/gel common to all the capillaries. Air pressure can be supplied to the reservoir through the periphery of each segment. Each segment has an integrated LED array board directly coupled to capillaries for providing excitation light. Optical fibers of a fiber bundle are individually guided through the periphery of each segment to the capillaries for emission collection. The fiber bundle delivers emissions to a detector that can read the emissions in a time-staggered multiplexed scheme. Each segment includes electrodes electrically coupled to a voltage source through the periphery of the segment for effecting CE separation.
    Type: Grant
    Filed: January 21, 2003
    Date of Patent: April 24, 2007
    Assignee: Biocal Technology, Inc.
    Inventors: Varouj Amirkhanian, Ming-Sun Liu, Paul Mooney