Abstract: A diagnostic system that includes a cartridge and a reader is discussed herein. The cartridge can contain a patient sample, such as a blood sample. The cartridge is inserted into the reader and the patient sample is analyzed. The reader contains various analysis systems, such as an electrophoresis detection system that uses electrophoresis testing to identify and quantify various components of the blood sample. The reader can process data from the various patient sample analysis to provide interpretative results indicative of a disorder, condition, disease, or infection of the patient.

Abstract: The present disclosure provides devices, systems and methods for automated processing of biological samples. This disclosure provides a gel-frame cassette for an automated bioprocessing device, comprising: a gel-frame comprising: a front face, a back face, a frame comprising: two vertical side bars, each comprising a gel-frame holding hole, a top bar connecting the two vertical side bars, a bottom bar connecting the two vertical side bars and a hollow chamber enclosed by the two vertical side bars, the top bar, and the bottom bar; a front panel in contact with the front face of the gel-frame, the front panel comprising an expanded upper portion, and a back panel in contact with the back face of the gel-frame, the back panel comprising a horizontal opening at the bottom of the back panel. This disclosure also provides an automated bioprocessing machine that processes the gel-frame cassette or the gel-frame.

Abstract: A device and method are useful for automated extraction of nucleic acids. The device can include a body that can be immersed partly or completely in a reaction cavity, where the part immersed in the reaction cavity has a non-smooth surface. After lysis, an organic substance, preferably alcohols or ketones, can be mixed with a biological sample. This mixture can be brought into contact with a material with a non-smooth surface. Under these conditions, nucleic acids may be adsorbed on the surface of the material being used. Washing steps may be carried out. After drying, the adsorbed nucleic acid may be detached from the material by adding water or a buffer of low salt concentration, whereupon it can be used for downstream applications.

Abstract: The present disclosure belongs to the field of display technology, and particularly relates to a display device. The display device is divided into a plurality of pixel regions, in each of which a first electrode and a second electrode are provided oppositely, and an electrophoretic medium and charged particles are provided between the first electrode and the second electrode. A reflective medium layer is further provided in each pixel region, the reflective medium layer and the second electrode are provided on a same side of the electrophoretic medium and the charged particles. The second electrode is provided in only a part of the pixel region, and the reflective medium layer is provided at least in an area of the pixel region in which the second electrode is absent. The display device has high contrast by increasing brightness in white state display.

Abstract: One or more methods for sampling gingival metabolites and biomarkers.

Abstract: An electrophoretic apparatus includes a first electrode, a second electrode, an electrophoretic element which is interposed between the first electrode and the second electrode, and a pixel circuit which is connected to a scanning line and a data line, and which includes a first transistor configured to supply a first electric potential to the first electrode, a second transistor configured to supply a second electric potential to the first electric potential, a third transistor configured to supply a third electric potential to the first electrode; a fourth transistor configured to supply a signal supplied through the data line to the first transistor, a fifth transistor configured to supply a signal supplied through the data line to the second transistor, and a sixth transistor configured to supply a signal supplied through the data line to the third transistor.

Abstract: In some embodiments, a product, such as a thermoset, has a polyhexahydrotriazine and a self-polymerized cross-linkable polymer. In some embodiments, a product is the reaction product of a diamine, an aldehyde, and a compound having an ?,?-unsaturated electron withdrawing moiety.

Abstract: A multi-color electrophoretic medium contains first, second and third species of particles. The first species of particles is light-scattering, while the second and third species of particles are transmissive. A method for driving such a display is also described.

Abstract: A fluid control and processing system for controlling fluid flow among a plurality of chambers comprises a body including a fluid processing region continuously coupled fluidicly with a fluid displacement region. The fluid displacement region is depressurizable to draw fluid into the fluid displacement region and pressurizable to expel fluid from the fluid displacement region. The body includes at least one external port. The fluid processing region is fluidicly coupled with the at least one external port. The fluid displacement region is fluidicly coupled with at least one external port of the body. The body is adjustable with respect to the plurality of chambers to place the at least one external port selectively in fluidic communication with the plurality of chambers.

Abstract: A screen is provided with microcapsules and a base material. The microcapsules each contain encapsulated liquid inside a capsule membrane. The microcapsules are planarly arranged on the base material. The encapsulated liquid includes light diffusion particles for scattering light, and a dispersion medium for dispersing the light diffusion particles. In a cross sectional area of the microcapsule projected in the direction perpendicular to the surface of the base material on which the microcapsules are planarly arranged, the ratio of the area of the encapsulated region to the area of the microcapsule ranges from 0.9025 to 0.990.

Abstract: An electrophoresis system is provided. The system includes a buffer chamber box having a front surface. The buffer chamber box is divided into a first chamber and a second chamber by a divider. The system also includes a gel plate. A gel chamber is formed by the gel plate and the front surface of the buffer chamber box. The first chamber has a first electrode and a first conductive path member. The second chamber has a second electrode and a second conductive path member.

Abstract: The present invention relates to, among other things, a gel electrophoresis system for detecting the level of specific Apolipoproteins and/or lipoprotein particles present in intact lipid particles in a biological sample. The system includes a gel substrate to receive a biological sample, at least two lipoprotein-binding complexes. Each complex includes an antibody that binds a lipoprotein particle or a portion thereof, which is bound to a signal producing molecule capable of producing or causing production of a detectable signal. The system also includes a device for detecting the detectable signal. The present invention also relates to methods of assessing the level of specific Apolipoproteins and/or lipoprotein particles present in a biological sample, determining whether a subject is at increased risk for cardiovascular disease, and monitoring the risk for developing cardiovascular disease.

Abstract: The disclosure is directed toward driving methods and a driving circuit which are particularly suitable for bi-stable displays. In certain embodiments, methods provide the fastest and most pleasing appearance to the desired image while maintaining the optimal image quality over the life expectancy of an electrophoretic display device.

Abstract: The present disclosure provides an isolated particle comprising very high density, ultra small, lipid depleted apo B containing particles, and may also contain cytokeratin 8. The isolated particle is useful in diagnostic assays, which are also provided.

Abstract: Electrophoresis tray arranged to support an electrophoresis cassette for miming electrophoresis experiments, the electrophoresis cassette comprising a gel member in a housing with a front and a back face, wherein the tray comprises a cassette support surface for supporting at least the separation zone of the electrophoresis cassette during electrophoresis, and wherein the cassette support surface is flanked by a pair of buffer pad holders each one arranged to hold a buffer pad in a mating position with respect to buffer connection sections at the back face of the electrophoresis cassette.

Abstract: A sample separation apparatus (200) for separating a fluidic sample, the sample separation apparatus (200) comprising a first separation unit (204) for separating the fluidic sample, a first fluid drive (202) configured for conducting the fluidic sample to be separated through the first separation unit (204), a second separation unit (208), arranged downstream of the first separation unit (204), for further separating the fluidic sample after treatment by the first separation unit (204), a second fluid drive (206) configured for at least partially conducting the fluidic sample, after treatment by the first separation unit (204), through the second separation unit (208), and a fluidic valve (218) having fluidic interfaces (222, 224, 226, 228) fluidically coupled to the first fluid drive (202) and the second fluid drive (206) and being switchable for performing the separation of the fluidic sample, wherein the sample separation apparatus (200) is configured for adjusting a pressure at a predefined position to a

Abstract: The invention relates to, among other things, a method for performing electrophoresis with in-situ calibration. The method includes combining a volume of a test sample with a volume or quantity of a calibrating sample to form a final volume, where the volume of the calibrating sample includes a known concentration of a calibrator and the final volume includes a known ratio of test sample to calibrating sample. The method also includes depositing a loading fraction in a receiving well of an electrophoretic gel, in which the loading fraction is a fraction of the final volume, and separating the loading fraction along a common separation lane of the electrophoretic gel such that components of the test sample and the calibrator are separated from one another along the common separation lane.

Abstract: The present invention provides a mechanism for separating or isolating charged particles under the influence of an electric field without metal electrodes being in direct contact with the sample solution. The metal electrodes normally in contact with the sample are replaced with high conductivity fluid electrodes situated parallel and adjacent to the sample. When the fluid electrodes transmit the electric field across the sample, particles within the sample migrate according to their electrophoretic mobility.

Abstract: Staining compositions and methods for use with a matrix, such as an electrophoretic gel, containing separated biopolymers. The compositions including an acid, an organic solvent, and a generally planar, fluid-permeable gel contact sheet consisting primarily of a non-cellulosic material. The acid and organic solvent may be sorbed to the fluid-permeable gel contact sheet, or may be sorbed to a layers contactable with a non-gel contact side of the gel contact sheet. In one embodiment, the composition is a source electrophoretic stain composition including a staining reagent. In one embodiment, the composition is a sink electrophoretic stain composition including a sorbent.

Abstract: A cylindrical cam device includes an axle and a plurality of disk portions disposed about the axle. The plurality of disk portions define a plurality of interstitial regions disposed between the plurality of disk portions. Each of the plurality of disk portions comprises a radial edge. The radial edge of each of the plurality of disk portions comprises a material or structure capable of retaining a liquid and releasing at least a portion of the liquid onto a substrate. A method for facilitating electrophoretic analysis of biological samples utilizing the cylindrical cam device is also disclosed.

Abstract: Separation methods based on electrophoresis or chromatography that use a stationary phase including fibril cellulose.

Abstract: A coated pigment that includes polymer encapsulations which act as a pigment dispersant and film forming agent, coating systems that include the coated pigment and methods for producing the coated pigment and the coating system are described. The polymer encapsulations of the coated pigments allow the coated pigments that are included in the coating system to be dispersed without the addition of any other dispersants and/or resins. Thus, the disclosed coated pigments simplify the process of making coating systems. The disclosed coated pigments also extend the shelf-life of the coating systems, and provide a final coating with enhanced pigment orientation and aesthetics.

Abstract: A gel cassette adaptor for use with an electrophoresis system gel cassette is disclosed herein. The gel cassette adaptor allows a single gel cassette to be compatible with more than one electrophoresis system. In one example embodiment, the gel cassette adaptor includes an open sided trough having an attachment perimeter and an attachment flange coupled to the attachment perimeter. The attachment flange is designed to be compatible for mating with a gel cassette that is compatible with a first electrophoresis system. After attachment of the gel cassette adaptor, the resulting gel cassette assembly is compatible with a second electrophoresis system. Also provided is a gel cassette that includes a stabilizer post that connects the cassette plates to maintain a constant gap width between the plates during electrophoresis. In reducing deformation of the gap space between plates during gel runs, the stabilizer post can improve electrophoresis results for wide format gels.

Abstract: A kit for separating and concentrating nucleic acid and protein targets includes labeled reagents which affect simultaneous co-purification and concentration of a nucleic acid and a protein, a gel isotachophoresis separation unit to which a sample comprising the nucleic acid and the protein is added, a detection unit for the detection of the presence of the nucleic acid and the protein, and instructions for use. The gel electrophoresis includes a gel box having a negative electrode side and a positive electrode side, the negative electrode side being filled with a first buffer comprising 2-Hydroxy-N-(tris(hydroxymethyl)methyl)-3-aminopropanesulfonic acid buffer and the positive electrode side being filled with a second buffer being different than the first buffer. The gel isotachophoresis separation is configured to subject the sample to isotachophoresis using a voltage.

Abstract: A system of controlled translocation of macromolecules by gel electrophesis employs a funnel nanopore structure. A graphene portion is attached to a porous material layer including funnel-shaped pores such that the graphene portion blocks the side of the porous material layer having openings for smaller pores. A pair of electrical contacts is formed on the graphene portion. A dielectric material layer may be deposited to hold the graphene portion in place. A nanoscale hole is formed through the dielectric material layer and the graphene portion to provide a smallest opening in a funnel nanopore structure. The funnel nanopore structure is placed within a capsule configured for gel electrophoresis. A linear chain of molecules can pass through a funnel-shaped pore and the nanoscale hole during the gel electrophoresis. A graphene nanopore detector allows measurement of blockage current for sufficient resolution of base pairs in DNA's.

Abstract: A method of screening compounds or molecules comprising the steps of: translating a sequence encoding the amino acid sequence comprising SEQ ID No. 29 in a translation system in the presence of a test compound or molecule; and analysing the translation product(s) for the presence of one or more of (a) a peptide comprising the amino acids Pro-Gly at the C terminus and a peptide comprising the amino acid Pro at the N terminus: or (b) a peptide comprising the amino acid sequence of SEQ ID No. 29.

Abstract: A channel (12) is formed inside a transparent substrate (11), and the two ends are respectively connected to a sample inlet (13) and a separation medium filling port (14) both open to outside, and a sample outlet (15) which is open to outside is provided on the channel (12). The portion between the sample inlet (13) and the sample outlet (15) is a separation channel portion (12a), and the portion between the sample outlet (15) and the separation medium filling port (14) is a separation medium introduction channel portion (12b). A separation medium supplier is connected to the separation medium filling port (14) and the separation medium introduction channel portion (12b) is filled with a separation medium. Then a sample is dropped to the sample inlet (13), with the sample medium supplier still connected, to introduce the sample to the separation channel portion (12a). And then, buffer liquid is poured in buffer reservoirs (16) and (17) and a migration voltage is applied to perform a migration analysis.

Abstract: The present invention provides an arsenite oxidase enzyme modified to prevent translocation by modification of a translocation signal sequence. A microorganism modified to express the heterologous arsenite oxidase enzyme is also provided by the invention, together with a device for detecting the presence of arsenite in a sample.

Abstract: A sample separation and adsorption appliance (100) includes a negative electrode (2), a positive electrode (3), a sample separation unit (6) that has a first opening (17) opened to a side facing the negative electrode (2) and a second opening (18) opened to a side facing the positive electrode (3), the sample separation unit containing a separation gel (7), and a slit structure (8) including a slit (1) at a position facing the second opening (18). A transfer film (9) is arranged between the second opening (18) and the slit (1).

Abstract: To provide a system by which evaluation of circumstances of contamination by microparticles having nucleic acid can be performed rapidly and accurately. The theme is achieved by a system for measuring microparticles that includes: (1) a microparticle adhesion step of adhering the microparticles having nucleic acid to a microparticle adhesion member; (2) a membrane breakage step of breaking membranes of the adhered microparticles by electrical discharge; (3) an electrophoresis step of electrophoresing the microparticles in a thickness direction of a gel to make the nucleic acid in the microparticles migrate from a negative electrode side toward a positive electrode side and adhere the nucleic acid on a surface of a nucleic acid detection member; and (4) a nucleic acid measurement step of fluorescently staining the surface of the nucleic acid detection member to measure a concentration of the nucleic acid.

Abstract: Novel lactoferrin fragments that are characteristic of inflammation during resolution and uses thereof. Diagnostic compositions and methods for assessing the presence or absence of resolving inflammation and for monitoring the progression of inflammatory resolution in a subject. Methods for treating a subject having an inflammatory disease, the methods including determining whether the subject has inflammation in resolution by determining the molecular weight of lactoferrin fragments.

Abstract: A chip (10) for electrophoresis is provided, the chip including a gel (9) composed of a polymer prepared by polymerizing a monomer, and a support (1) configured to support the gel (9). In the chip (10) for electrophoresis, a surface of the support (1) in contact with the gel (9) is covered with a surface treatment compound (4) containing the monomer or a derivative of the monomer.

Abstract: Particles of interest, such as DNA molecules, are injected into a medium by applying a first field. Once in the medium the particles are concentrated by applying one or more fields that cause mobilities of the particles in the medium to vary in a manner that is correlated with motions of the particles. Particle injection and particle concentration may be performed concurrently or in alternation.

Abstract: To provide a reagent applying device and a reagent applying method for electrophoresis analysis which make it possible to apply a reagent with a simple configuration at low cost. A reagent supplying device for supplying a reagent to the surface of a gel in electrophoresis analysis includes a reagent applying tool which is a plate-shaped body. The reagent applying tool includes at least one reagent holding section which penetrates the reagent applying tool in a thickness direction and which holds a reagent by capillary action, and a reagent spreading section for spreading, on the surface of the gel, a reagent supplied from a lower-side opening of the reagent holding section when the reagent applying tool is placed on the gel.

Abstract: A device comprises an electric field applying assembly adapted to generate an electric field having a discrete electric field profile; a conducting volume and an electrical interface region provided between the conducting volume and the electric field applying assembly such that the discrete electric field is applied to the material by the electric field applying assembly at a location spaced from the conducting volume, wherein the electrical interface region comprises at least an ionically conductive material arranged adjacent to an in contact with the conducting volume; such that the discrete electric field applied by the electric field applying assembly is smoothed by the electrical interface region so that the electric field profile established within the conducting volume is substantially continuous.

Abstract: Electrophoretic systems, formulations and methods are described which allow a user to perform electrophoresis experiments under conditions of high voltage and with reduced run time. An electrophoretic system, formulation or method may be run at 50% higher field strength than comparable systems already in use in the art. The presently described systems and formulations may be run at voltages above 225 V, above 250 V, above 275 V, above 300 V, above 325 V or above 350 V. The time required for performing an electrophoresis experiment may be reduced to less than about 30 minutes, less than about 20 minutes, less than about 15 minutes or less than about 12 minutes.

Abstract: Two-dimensional gel electrophoresis apparatus includes an electrophoresis zone having four edges defined by a plurality of electrodes. A pair of opposed edges are defined by groups of discrete electrodes. Discrete electrodes within each group are electrically isolated from each other while the other pair of opposed edges are used to generate an electrical field. As a result, the electrical field is less distorted than would be the case if each edge was defined by a single elongate electrode. The apparatus can be provided as a cassette with electrodes configured to guide gas generated during electrolysis out of the cassette through apertures, to reduce the build up of combustible gases.

Abstract: Gels, such as polyacrylamide gels, are provided that include linear polyacrylamide in the stacking gel. Native gels that include linear polyacrylamide in the stacker can be used to separate biomolecular complexes, such as protein complexes. Gel cassettes in which the gap width between front and back plates does not vary by more than 5% at the upper edge of the cassette are also provided. The gel cassettes can be used for electrophoretic separation of proteins and protein complexes on native gels, such as native gels that include linear polyacrylamide in the stacker. The native gels can have multiple wells for electrophoresing at least one sample and/or at least one molecular weight standard.

Abstract: A device and method for displaying grey levels on electronic paper is provided. According to various embodiments, a system for electronic paper can include an electret substrate embedded with at least one first capsule containing a first plurality of charged pigment particles and at least one second capsule containing a second plurality of charged pigment particles. The system can further include a first electrode interfacing with one side of the electret substrate and a second electrode interfacing with a second side of the electret substrate. The first plurality of charged pigment particles can move in the direction of one of the first and second electrodes having a polarity that is opposite to that of the first plurality of the charged pigment particles in response to a voltage applied to the first and second electrodes that is greater than a first threshold.

Abstract: The invention provides an electroblotting system for blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, an ion source juxtaposed with the anode between the anode and the transfer stack, a cathode, and another ion source juxtaposed with the cathode between the cathode and the transfer stack, in which the each ion source is sufficient for electrophoretic transfer. The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.

Abstract: A system for collecting target nucleic acids from a sample can include at least one sample chamber configured to receive a sample containing target nucleic acids and other material, at least one collection chamber removably mountable relative to the at least one sample chamber and configured to collect target nucleic acids separated from the other material, a filter removably mountable relative to the at least one sample chamber and configured to be disposed between the at least one sample chamber and the at least one collection chamber when the at least one collection chamber is mounted relative to the at least one sample chamber.

Abstract: Cassette electrophoresis systems that allow viewing of molecules during the electrophoresis run are disclosed. Cassette electrophoresis bases that reversibly engage light sources, such as light source bases are disclosed. Also disclosed are visible light transillumination systems for viewing a pattern of fluorescence emitted by fluorophores comprising a cassette housing fluorophore-containing material and a base unit to support the cassette. In some aspects the base unit that includes a power supply also houses a light source having output in the visible wavelength region and a filter placed between the light source and the fluorophores. The system is constructed and arranged such that patterns of fluorescence emitted by the fluorophores are viewable. Also described are charging devices for providing charge to gel electrophoresis systems, portable gel electrophoresis systems and methods of use thereof.

Abstract: A method of actuating, comprising: filling at least a portion of a tube (21) with a liquid (19) containing electrolytes, the tube (21) having an inner surface that is electrically chargeable when in contact with the liquid (19); positioning an object (28) in fluid communication with the liquid in the tube; and applying an electrical field (46) along a lengthwise axis across the tube at said portion for producing a pressure in the liquid. The pressure in the liquid exerts a force on the object so as to actuate the object (28, 30). An actuator (20) is also disclosed.

Abstract: Provided herein is a surface acoustic wave (“SAW”) sensor device including an isolation component of a target biomolecule. A sample containing the target biomolecule is separated by its size using electrophoresis, and sequentially reacts with a SAW sensor. In other words, the device is capable of detecting the target biomolecule by separating biomolecules using electrophoresis, and applying the separated biomolecules to the SAW sensor.

Abstract: Various embodiments provide, for example, buffer compositions and/or sieving formulations useful in connection with electrophoresis instruments, such as capillary electrophoresis (CE) devices. In various embodiments, a buffer composition can include Bis-Tris, TAPS and/or TAPSO, and, optionally, a chelating agent, such as EDTA. Methods of separating samples containing bio-molecules, such as DNA or RNA, are also described.

Abstract: Polyacrylamide gels that offer high resolution in protein separations and are more stable relative to hydrolysis than conventional polyacrylamide gels that rely on Tris or Tris-Bis as buffering agents are made by incorporating triethanolamine in place of most or all of the Tris or Tris-Bis.

Abstract: Provided herein are methods useful in detecting degradative enzymes in bodily fluid samples.

Abstract: A gel cassette adaptor for use with an electrophoresis system gel cassette is disclosed herein. The gel cassette adaptor allows a single gel cassette to be compatible with more than one electrophoresis system. In one example embodiment, the gel cassette adaptor includes an open sided trough having an attachment perimeter and an attachment flange coupled to the attachment perimeter. The attachment flange is designed to be compatible for mating with a gel cassette that is compatible with a first electrophoresis system. After attachment of the gel cassette adaptor, the resulting gel cassette assembly is compatible with a second electrophoresis system. Also provided is a gel cassette that includes a stabilizer post that connects the cassette plates to maintain a constant gap width between the plates during electrophoresis. In reducing deformation of the gap space between plates during gel runs, the stabilizer post can improve electrophoresis results for wide format gels.

Abstract: A nanopore device including a nanopore formed by penetrating a thin layer, a nanochannel formed at an entrance of the nanopore, and a filler in the nanochannel, as well as a method of fabricating the nanopore device and an apparatus including the nanopore device.

Abstract: A slide holder assembly and method for use in high content screening of a comet assay. The slide holder assembly has an inside surface and is configured to receive a slide holding a plurality of biological cells on a top surface thereof. When the slide is secured within the slide holder assembly, the inside surface of the slide holder assembly and the top surface of the slide combine to form a cavity having an open top end. The cavity is watertight and configured to hold a liquid therein. A method of performing a comet assay using a slide disposed within a slide holder assembly. The method includes performing encapsulation, lysis, electrophoresis, staining, and imaging of cells on the slide while the slide remains secured within the slide holder assembly.