Blotter (e.g., Membrane, Etc.) Patents (Class 204/614)
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Patent number: 11474121Abstract: The present invention provides a device and a method for automated liquid exchange in a horizontally rotating container that may be applied in automated sample treatment by using chemical solutions, in sequential order. Such applications include biomolecule staining on solid support or fixed samples such as western blot or tissue slice processing. Such sequential chemical reactions are usually performed on flat surfaces. Alternatively, these reactions can be completed with much less volume of reagents in horizontally rotating containers. Yet, it is difficult to automate these processes in rotating containers since the required tubing would twist due to the continuous rotation of the container. The present invention solves the problem of twisting of tubing by leading them through a stationary tubing carrier that passes through the rotating container.Type: GrantFiled: May 20, 2019Date of Patent: October 18, 2022Assignee: SMARTRESEARCH DOO BEOGRAD-ZVEZDARAInventor: Marko Panic
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Patent number: 10737268Abstract: Devices and methods are provided for the separation and dispensing of material using a microfluidic separation column connected via an exit channel to one or more sheath flow channels. The flow of separated material through the separation column is at least partially driven by a voltage potential between a first electrode within the separation column and a terminating electrode within at least one of the sheath flow channels. The separation column, exit channel, sheath flow channels, and electrodes are all within a single monolithic chip. The presence of an on-chip terminating electrode allows for separated material to be entrained in the sheath fluids and ejected onto a surface that can be non-conductive. The presence of multiple sheath flows allows for sheath flow fluids to have different compositions from one another, while reducing the occurrence of sheath flow fluids entering the separation column.Type: GrantFiled: August 7, 2017Date of Patent: August 11, 2020Assignee: LI-COR, INC.Inventors: Michael D. Furtaw, Donald T. Lamb, Lyle R. Middendorf
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Patent number: 9945808Abstract: Electrotransfer is performed in an instrument that receives electroblotting cassettes and that contains an integrated power supply, controls, and a display that allows the user to monitor and control each of a plurality of cassettes individually through electrical contacts within the housing that mate with corresponding electrical contacts on the cassettes.Type: GrantFiled: October 22, 2015Date of Patent: April 17, 2018Assignee: BIO-RAD LABORATORIES, INC.Inventors: Matthew Latham, William Strong, Nikolas Chmiel, Jeff Xu, Robert Iovanni
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Patent number: 9128046Abstract: A slide holder assembly and method for use in high content screening of a comet assay. The slide holder assembly has an inside surface and is configured to receive a slide holding a plurality of biological cells on a top surface thereof. When the slide is secured within the slide holder assembly, the inside surface of the slide holder assembly and the top surface of the slide combine to form a cavity having an open top end. The cavity is watertight and configured to hold a liquid therein. A method of performing a comet assay using a slide disposed within a slide holder assembly. The method includes performing encapsulation, lysis, electrophoresis, staining, and imaging of cells on the slide while the slide remains secured within the slide holder assembly.Type: GrantFiled: October 23, 2012Date of Patent: September 8, 2015Assignee: CELLOMICS, INC.Inventors: Brent A. Samson, Chandrasekaran Vasudevan, Keith R. Heffley, Joseph M. Zock, Dirk John Vandenberg, III
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Publication number: 20140339084Abstract: Electrotransfer is performed in an instrument that receives electroblotting cassettes and that contains an integrated power supply, controls, and a display that allows the user to monitor and control each of a plurality of cassettes individually through electrical contacts within the housing that mate with corresponding electrical contacts on the cassettes.Type: ApplicationFiled: March 28, 2014Publication date: November 20, 2014Applicant: BIO-RAD LABORATORIES, INC.Inventors: Matthew Latham, William Strong, Nikolas Chmiel, Jeff Xu, Robert Iovanni
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Patent number: 8721860Abstract: A technique for producing multiple protein blots from a single gel, including entering the number ‘n’ membranes to be blotted into microprocessor memory, determining calibration constants for a gel batch, inputting calibration constants into microprocessor memory, and calculating ‘n’ voltage/time profiles for simultaneous blotting. A gel layer from the gel batch is treated with a protein sample, ‘n’ membranes are placed onto the gel layer to yield a gel pack, and the gel pack is placed between an electrode plate maintained at a fixed first voltage and an array of ‘n’ spaced generally parallel electrodes. The voltage to each respective ‘n’ spaced generally parallel electrodes is varied over time according to a respective ‘n’ voltage/time profile to transfer proteins to each respective ‘n’ membranes to yield blotted membranes to yield ‘n’ respective blotted membranes.Type: GrantFiled: October 26, 2011Date of Patent: May 13, 2014Assignee: Indiana University Research and Technology CorporationInventors: Stanley Yung-Ping Chien, Hiroki Yokota
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Patent number: 8715476Abstract: Electrotransfer is performed in an instrument that receives electroblotting cassettes and that contains an integrated power supply, controls, and a display that allows the user to monitor and control each of a plurality of cassettes individually through electrical contacts within the housing that mate with corresponding electrical contacts on the cassettes.Type: GrantFiled: December 8, 2010Date of Patent: May 6, 2014Assignee: Bio-Rad Laboratories, Inc.Inventors: Matthew Latham, William Strong, Nikolas Chmiel, Jeff Xu, Robert Iovanni
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Patent number: 8702950Abstract: A device for electrophoresis applies a voltage to a medium in contact with a plurality of electric conductors so that a potential of adjacent conductors is within a certain range. This allows preventing decline in electrophoresis speed. A device for electrophoresis and transfer includes an electrode having a plurality of electrode regions being insulated one another and arranged in a specific direction. This allows providing a practical and easy-to-use device for electrophoresis and transfer. A device for transfer alters an applied voltage or applied voltage duration to a certain position to another position. This allows improving transfer efficiency.Type: GrantFiled: September 10, 2012Date of Patent: April 22, 2014Assignees: Sharp Kabushiki Kaisha, Toppan Printing Co., Ltd.Inventors: Yuji Maruo, Yutaka Unuma, Atsunori Hiratsuka, Hideki Kinoshita, Kenji Yokoyama, Koji Sakairi, Satonari Akutsu
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Patent number: 8568580Abstract: A system for collecting target nucleic acids from a sample can include at least one sample chamber configured to receive a sample containing target nucleic acids and other material, at least one collection chamber removably mountable relative to the at least one sample chamber and configured to collect target nucleic acids separated from the other material, a filter removably mountable relative to the at least one sample chamber and configured to be disposed between the at least one sample chamber and the at least one collection chamber when the at least one collection chamber is mounted relative to the at least one sample chamber.Type: GrantFiled: July 24, 2008Date of Patent: October 29, 2013Assignee: Applied Biosystems, LLCInventors: Charles S. Vann, Maxim G. Brevnov, David W. Ruff, Kenneth J. Livak
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Publication number: 20130248370Abstract: Disclosed are methods and apparatus for separation of biomolecules via two-dimensional gel electrophoresis, methods and apparatus for immunoblotting separated biomolecules, and methods for the use of biomolecules processed via the methods and apparatus of the present invention, including use in a clinical setting. The methods and apparatus for separation of biomolecules via two-dimensional gel comprises vertical agarose gel electrophoresis in the first dimension, and the electrophoresis of a novel non-denaturing 3-35% concave gradient polyacrylamide gel in the second dimension. This novel gel can be cast in a modified gel caster that can facilitate the pouring of multiple gels simultaneously. The methods and apparatus for immunblotting are useful with any type of immunoblotting, including Western blot, Northern blot, and Southern blot analyses.Type: ApplicationFiled: May 17, 2013Publication date: September 26, 2013Applicant: BOSTON HEART DIAGNOSTICS CORPORATIONInventors: Bela F. Asztalos, Ernst J. Schaefer
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Publication number: 20130140183Abstract: The disclosed device for trapping biologically-relevant substances can easily collect biologically-relevant substances from a thin tissue section or a gel in which said biologically-relevant substance have been fractionated. Said device is provided with: trapping bodies for trapping biologically-relevant substances; carriers that hold the trapping bodies; and electrodes for charging the trapping bodies. The trapping bodies are charged and brought into contact with a prescribed position on a thin tissue section that contains biologically-relevant substances or a gel in which biologically-relevant substances have been fractionated, thereby trapping said biologically-relevant substances from said gel or thin tissue section.Type: ApplicationFiled: June 22, 2011Publication date: June 6, 2013Inventor: Hideji Tajima
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Patent number: 8444839Abstract: Electroblotting for the transfer of electrophoretically separated species from a gel to a transfer membrane is performed in a semi-dry format with sheets of absorbent polyester or polyester/cellulose blend wetted with buffer solution in place of the traditional buffer-wetted filter paper. The result is effective electroblotting at a lower electric current level than that obtained with filter paper and thereby less resistance heating of the gel, the transfer membrane, and the species being transferred.Type: GrantFiled: December 12, 2011Date of Patent: May 21, 2013Assignee: Bio-Rad Laboratories, Inc.Inventors: Xuemei Yang, Cory M. Panattoni
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Patent number: 8357278Abstract: An electrotransfer cassette is formed in two parts that are releasably joined by a manually operated locking mechanism. The joined parts have electrical contact areas extending from the electrodes in the two parts of the cassette, the contact areas being exposed on an outer edge of the resulting cassette to form electrical connections to a power supply upon the simple insertion of the cassette into an instrument.Type: GrantFiled: December 8, 2010Date of Patent: January 22, 2013Assignee: Bio-Rad Laboratories, Inc.Inventor: Matthew Latham
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Patent number: 8268149Abstract: The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.Type: GrantFiled: August 21, 2006Date of Patent: September 18, 2012Assignee: Life Technologies CorporationInventors: Ilana Margalit, Uri Yogev, Itay Sela, Yuri Katz, Adam Sartiel
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Publication number: 20120211364Abstract: The invention provides an electroblotting system for blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, an ion source juxtaposed with the anode between the anode and the transfer stack, a cathode, and another ion source juxtaposed with the cathode between the cathode and the transfer stack, in which the each ion source is sufficient for electrophoretic transfer. The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.Type: ApplicationFiled: February 15, 2012Publication date: August 23, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Ilana Margalit, Uri Yogev, Itay Sela, Yuri Katz, Adam Sartiel
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Publication number: 20120138465Abstract: The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.Type: ApplicationFiled: February 10, 2012Publication date: June 7, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Ilana MARGALIT, Uri YOGEV, Itay SELA, Yuri KATZ, Adam SARTIEL
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Patent number: 8173002Abstract: The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.Type: GrantFiled: February 21, 2006Date of Patent: May 8, 2012Assignee: Life Technologies CorporationInventors: Ilana Margalit, Uri Yogev, Itay Sela, Yuri Katz, Adam Sartiel
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Publication number: 20120090999Abstract: Electroblotting for the transfer of electrophoretically separated species from a gel to a transfer membrane is performed in a semi-dry format with sheets of absorbent polyester or polyester/cellulose blend wetted with buffer solution in place of the traditional buffer-wetted filter paper. The result is effective electroblotting at a lower electric current level than that obtained with filter paper and thereby less resistance heating of the gel, the transfer membrane, and the species being transferred.Type: ApplicationFiled: December 12, 2011Publication date: April 19, 2012Applicant: BIO-RAD LABORATORIES, INC.Inventors: Xuemei Yang, Cory M. Panattoni
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Publication number: 20120000781Abstract: The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.Type: ApplicationFiled: June 29, 2011Publication date: January 5, 2012Applicant: LITE TECHNOLOGIES CORPORATIONInventors: Ilana MARGALIT, Uri Yogev, Itay Sela, Yuri Katz, Adam Sartiel
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Patent number: 8075755Abstract: Electroblotting for the transfer of electrophoretically separated species from a gel to a transfer membrane is performed in a semi-dry format with sheets of absorbent polyester or polyester/cellulose blend wetted with buffer solution in place of the traditional buffer-wetted filter paper. The result is effective electroblotting at a lower electric current level than that obtained with filter paper and thereby less resistance heating of the gel, the transfer membrane, and the species being transferred.Type: GrantFiled: December 13, 2007Date of Patent: December 13, 2011Assignee: Bio-Rad Laboratories, Inc.Inventors: Xuemei Yang, Cory M. Panattoni
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Patent number: 7749369Abstract: The present invention relates to a gel processing and transfer device, useful for the processing and transferring un-damaged and intact gel with minimal handling, said device comprising at least 4 separable components namely: a base plate for holding the gels with facility to drain out solution; a retaining rim with attached side-walls, said side walls are fastened to the base plate by a fastening means; at least one “O” ring fixed to the retaining rim to give leakproof arrangement with the base plate; and a lid to cover the assembly.Type: GrantFiled: February 17, 2006Date of Patent: July 6, 2010Inventors: Sanjay Kumar, Dhiraj Vyas
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Publication number: 20100089754Abstract: To provide a system by which evaluation of circumstances of contamination by microparticles having nucleic acid can be performed rapidly and accurately. The theme is achieved by a system for measuring microparticles that includes: (1) a microparticle adhesion step of adhering the microparticles having nucleic acid to a microparticle adhesion member; (2) a membrane breakage step of breaking membranes of the adhered microparticles by electrical discharge; (3) an electrophoresis step of electrophoresing the microparticles in a thickness direction of a gel to make the nucleic acid in the microparticles migrate from a negative electrode side toward a positive electrode side and adhere the nucleic acid on a surface of a nucleic acid detection member; and (4) a nucleic acid measurement step of fluorescently staining the surface of the nucleic acid detection member to measure a concentration of the nucleic acid.Type: ApplicationFiled: December 17, 2009Publication date: April 15, 2010Applicant: Toyohashi University of TechnologyInventors: Akira MIZUNO, Kazunori Takashima, Hachiro Yasuda, Masudur Rahman
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Patent number: 7495231Abstract: The invention provides an apparatus for producing an image of a global surface of an ion source sample plate that is exterior to an ion source. In general terms, the apparatus contains a sample plate for an ion source, an imaging device (e.g., a CCD or CMOS camera) and an illumination device that is configured to produce a light beam that contacts the sample plate surface to define a grazing angle between the light beam and the sample plate surface. The apparatus may be present at a location that is remote to the ion source.Type: GrantFiled: September 8, 2005Date of Patent: February 24, 2009Assignee: Agilent Technologies, Inc.Inventors: Jean-Luc Truche, Gregor T. Overney, William D. Fisher, Richard P. Tella
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Publication number: 20090026079Abstract: The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). The anode, the cathode, or both can be separate from a power supply and provided as part of a disposable electrode assembly that also includes a body of gel matrix that includes ions for electrophoretic transfer.Type: ApplicationFiled: July 11, 2008Publication date: January 29, 2009Applicant: INVITROGEN CORPORATIONInventors: Ilana MARGALIT, Uri Yogev, Itay Sela, Yuri Katz, Adam Sartiel, Timothy Updyke
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Patent number: 7435322Abstract: The invention relates to a device and a method for automatically analysing the constituents of at least one analyte, especially by means of gel electrophoresis and/or isoelectric focussing, comprising at least one separating matrix, especially a gel. Each separating matrix is applied on at least one side in a supporting direction S of a supporting element, can be loaded with an analyte, and can be electrically contacted on opposite ends in a separating direction T in order to split the analyte into its constituents.Type: GrantFiled: February 1, 2002Date of Patent: October 14, 2008Inventor: Hartmut Schlichting
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Patent number: 7384781Abstract: A sensor device is provided for detecting an analyte in a sample in which an analyte is bound to a detection reagent to form a bound complex. The device comprises (a) a sample (5) comprising an ionic analyte and a detection reagent in a conductive fluid, wherein the detection reagent has a net charge different from the analyte; (b) a first permeable polymeric hydrogel plate (3) and a first spacer plate (8), which plates provide a compartment for the sample; (c) an anode (1) juxtaposed to the outside of the first hydrogel plate and not in contact with the sample; (d) a cathode (9) juxtaposed to the outside of the first spacer plate and not in contact with the sample; (e) a voltage generator (10) to apply an electric potential to the anode and cathode; and (f) a detector (11).Type: GrantFiled: September 21, 2005Date of Patent: June 10, 2008Inventors: William R. Moyle, Russell C. Scaduto, Jr.
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Patent number: 7250097Abstract: An apparatus and method for the sequential electroelution of biomolecules is described, the apparatus comprising a separation medium having an outlet, and a collector having at least a first receptacle and a second receptacle that can be sequentially brought into contact with the outlet of the separation medium by translating the first receptacle and the second receptacle in relation to the outlet of the separation medium, and the method comprising the steps of receiving a first substantially separated molecule in the first receptacle and translating the first receptacle and the second receptacle such that the second receptacle is brought into in contact with the outlet of the separation medium, receiving a second substantially separated molecule in the second receptacle, and repeating said steps to sequentially receive a desired number of substantially separated molecules.Type: GrantFiled: July 9, 2004Date of Patent: July 31, 2007Assignee: The United States of America, Department of Health and Human ServicesInventors: József Antal, Zsuzsanna Buzás, Andreas Chrambach
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Patent number: 6833060Abstract: The present invention relates generally to the electrophoretic separation of biomolecules, including but not limited to proteins, peptides, DNA and RNA. The methods of this invention are particularly useful when applied to two-dimensional gel electrophoresis.Type: GrantFiled: May 7, 2001Date of Patent: December 21, 2004Assignee: University of Louisville Research Foundation, Inc.Inventors: George Harding, Elias Klein, Jon Klein
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Patent number: 6638408Abstract: The invention provides a novel solution isoelectric focusing device and method that can reproducibly fractionate charged molecules into well-defined pools. This approach can be applied to mixtures of charged molecules, such as eukaryotic proteome samples where reproducible resolution and quantitation of greater than 10,000 protein components is feasible.Type: GrantFiled: April 3, 2000Date of Patent: October 28, 2003Assignee: The Wistar InstituteInventors: David W. Speicher, Xun Zuo
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Patent number: 6632339Abstract: Polypeptides which have been separated by gel electrophoresis can be identified by electroblotting them through a “sandwich” comprising in order: a) the separation gel; b) at least one hydrophilic membrane, e.g. of carboxyl-modified PVDF, on which is immobilized at least one reagent capable of cleaving a polypeptide, e.g. trypsin; c) a hydrophobic layer, typically a membrane, e.g. of PVDF. Preferably a biased alternating current or discontinuous direct current is used for electroblotting. The resulting fragments, usually peptides, are identified, preferably by MALDI-TOF MS.Type: GrantFiled: June 15, 2000Date of Patent: October 14, 2003Assignee: University of GenevaInventors: Willy Vincent Bienvenut, Denis Francois Hochstrasser
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Patent number: 6592734Abstract: A semi-dry electroblotter for transferring a gel after its electrophoresis onto a membrane enabling the electro-blotting process to be efficiently and effectively carried out within a short time, eliminating the shortcomings of the traditional technology, includes an upper block, and a corresponding lower block. A respective positioning member is disposed on each of the lateral sides of the upper block and the lower block, such that the upper block and the lower block will be coupled automatically when they are closed in a correct guiding position. An elastic member and an electrode plate are separately disposed in the interior of the upper block and the lower block, and the elastic member supports the electrode plate so that the electrode plate is biased in a clamping direction.Type: GrantFiled: April 27, 2001Date of Patent: July 15, 2003Assignee: Wealtec Enterprise Co., Ltd.Inventor: Hui-Wan Chen
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Publication number: 20020166769Abstract: An apparatus and method are described for capillary separation of macromolecules and precise post-separation blotting. Apparatus include disposable separating element (capillary), which contains a sieving or interaction matrix inside, an external layer of blotting material, positioned close to the boundary of said sieving or interaction matrix, and the membrane with changeable permeability for separated material; said membrane separates blotting layer from the sieving or interaction matrix. After separation of macromolecules in capillary with initially non-permeable walls, chemical or physical modification of the membrane is performed, which is followed by changing the vector of driving forces for transfer, so that separated molecules are moved through the walls of the capillary and blotted to the outer layer of separating element, which contains blotting material. Means of modification of the membrane include chemical or physical modification, leading to changes in permeability.Type: ApplicationFiled: March 5, 2001Publication date: November 14, 2002Inventor: Vladimir B. Serikov
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Publication number: 20020157953Abstract: The present invention discloses a semi-dry electroblotter for transferring a gel after its electrophoresis onto a membrane enabling the electro-blotting process to be efficiently and effectively proceeded within a short time, and eliminating the shortcomings of the traditional technology. The electroblotter of the present invention comprises an upper block, and a corresponding lower block in the shape of a groove. A corresponding positioning member is disposed on each of the lateral sides of the upper block and the lower block, such that the upper block and the lower block will be coupled automatically when they are closed in a correct guiding position. An elastic member and an electrode plate are separately disposed in the interior of the upper block and the lower block, and the elastic member supports the electrode plate so that the electrode plate has an elastic reaction on the opposite direction when the electrode plate is pressed.Type: ApplicationFiled: April 27, 2001Publication date: October 31, 2002Inventor: Hui-Wan Chen
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Patent number: 6193868Abstract: An electrophoretic separating and blotting apparatus, which includes a pressure block, two glass plates of different heights, a partition frame, a rack, a positioning unit, a cell, a blotting cassette, and two electrode plates. The component parts are alternatively used for electrophoretic gelation as well as blotting, enabling electrophoretic gelation process and blotting process to be efficiently and economically proceeded within a short length of time.Type: GrantFiled: September 16, 1999Date of Patent: February 27, 2001Inventor: Yi-Hua Hsu
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Patent number: 5916429Abstract: In a horizontal gel, direct blot electrophoresis separation apparatus, device to remove gas dissolved in the buffer solution surrounding the anode at a location remote from the interface between the gel and the moving membrane, such as a gas evolution member disposed between the anode and the moving membrane of such apparatus, has been found to result in more consistent and readable blot patterns with less defects.Type: GrantFiled: August 1, 1997Date of Patent: June 29, 1999Assignee: Qualicon Inc.Inventor: Donald Harvey Brunk
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Patent number: 5840169Abstract: A process for electroelution of a gel containing charged macromolecules, such as proteins or polynucleotides, comprising the steps of providing a plurality of adjacent parallel chambers having a trapezoidal cross-section, placing a gel containing the charged macromolecules onto first open sides of the chambers, placing a semipermeable membrane onto second open sides of the chambers, filling the chambers with an elution buffer and applying a voltage difference across the chambers so that charged macromolecules in the gel migrate into the elution buffers in the chambers. Also, an apparatus for electroelution of a gel containing charged macromolecules having, preferably, a plurality of adjacent parallel chambers having a trapezoidal cross-section and vents for removing the product without disassembling the apparatus.Type: GrantFiled: March 13, 1995Date of Patent: November 24, 1998Inventor: Peter Andersen
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Patent number: 5635045Abstract: An apparatus for electroelution isolation of biomolecules and recovering biomolecules after elution including a tubular enclosure for engaging a piece of separating gel having a band of biomolecules. The enclosure is capped by a closure means having a passage means, and then placed in an electrophoresis tank with the closure means facing the positive terminal. Applying an electric current forces the biomolecules to accumulate at the closure means. The biomolecules may be recovered by inserting a capillary tip through the passage means and drawing the biomolecules through the capillary.Type: GrantFiled: March 31, 1994Date of Patent: June 3, 1997Inventor: Aftab Alam
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Patent number: 5614361Abstract: A method of characterizing an unknown organism species which comprises, determining the position of part or whole of evolutionarily conserved DNA sequences in DNA of the organism, relative to the position of restriction endonuclease cleavage sites in the DNA, thereby to obtain an identifying genetic characterization of the unknown organism, and comparing the characterization with information from at least two sets of identifying genetic characterizations derived from the same conserved sequences, each of the sets defining a known organism species.Type: GrantFiled: March 10, 1994Date of Patent: March 25, 1997Inventor: John A. Webster, Jr.
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Patent number: 5595636Abstract: The invention relates to the mass spectrometric analysis of separated substance samples on so-called 2-D-gel electrophoresis plates, used particularly for protein determination, with ionization of the substance samples by MALDI. The molecules of the substance samples are transferred to a thin, lacquer-like smooth matrix layer which should preferably be applied to a metal sample support. Transfer takes place directly or indirectly, for example via a polyvinylidene difluoride membrane an an intermediate carrier, by electrophoretic transport of the molecules to the matrix surface. Before transfer, the proteins may be subjected to enzymatic cutting of their amino acid chains.Type: GrantFiled: March 9, 1995Date of Patent: January 21, 1997Assignee: Bruker-Franzen Analytik GmbHInventor: Jochen Franzen
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Patent number: 5514256Abstract: This invention is a means for the rapid sequencing of DNA samples. More specifically, it consists of a new design direct blotting electrophoresis unit. The DNA sequence is deposited on a membrane attached to a rotating drum. Initial data compaction is facilitated by the use of a machined multi-channeled plate called a ribbon channel plate. Each channel is an isolated mini gel system much like a gel filled capillary. The system as a whole, however, is in a slab gel like format with the advantages of uniformity and easy reusability. The system can be used in different embodiments. The drum system is unique in that after deposition the drum rotates the deposited DNA into a large non-buffer open space where processing and detection can occur. The drum can also be removed in toto to special workstations for downstream processing, multiplexing and detection.Type: GrantFiled: October 28, 1994Date of Patent: May 7, 1996Assignee: Battelle Memorial InstituteInventors: Richard J. Douthart, Shannon L. Crowell