Abstract: The invention relates to the use of particles comprising binding ligands and electron transfer moieties (ETMs). Upon binding of a target analyte, a particle and a reporter composition are associated and transported to an electrode surface. The ETMs are then detected, allowing the presence or absence of the target analyte to be determined.

Abstract: In some examples, a device applies an electrical potential difference across a blood sample. The device measures an electrical signal that passes through the blood sample over a duration of time to obtain a plurality of measurements representing a measurement function of time. An accumulative property of the measurement function may be determined such that the accumulative property correlates to a blood coagulation characteristic.

Abstract: An electrochemical method for capsaicinoid and/or related compound detection in a sample by way of a screen printed electrode having a working electrode, a reference electrode, and a counter electrode. The working electrode can be screen-printed using conductive carbon ink; the reference electrode can be screen printed using conductive carbon ink; and the counter electrode can be screen printed using silver/silver chloride. The method includes contacting the electrode with the sample in the presence of electrolyte solution, and determining whether a change in redox potential occurs by way of differential pulse voltammetry, wherein a modulation amplitude is between approximately 0.1 volt/min and approximately 2.0 volt/min, a step potential is between approximately 0.0005 volt and approximately 0.01 volt, a modulation time is 0.05 seconds, and a corresponding interval time is approximately 0.5 second.

Abstract: The invention relates to a method of inhibiting the insertion of one or more membrane proteins into a lipid bilayer. The invention also relates to a method of inserting a pre-determined number of membrane proteins into a lipid bilayer and lipid bilayers having a pre-determined number of membrane proteins inserted therein. The lipid bilayers of the invention are useful as sensor arrays, particularly for sequencing nucleic acids.

Abstract: Described herein are systems and methods for detecting a target analyte in a sample with electrodes, comprising a linker and an antibody attached to the linker, and measuring an electrocatalytic signal changes generated by binding of an analyte in the sample to the antibody. Also disclosed herein are kits for electrochemical detection of protein analytes.

Abstract: The disclosed invention relates to an amperometric gas sensor for measuring the concentration of an analyte, comprising: a solid support; and a working electrode in contact with the solid support; wherein the analyte comprises a dopant which when in contact with the solid support increases the electrical conductivity of the solid support. A sterilization process employing the amperometric gas sensor is disclosed.

Abstract: A method an system is disclosed for the detection and/or allocation of at least one point mutation in target DNA and/or RNA duplexes. The method comprises obtaining a functionalized surface which is coated with probe DNA and/or RNA whereto target DNA and/or RNA duplexes are attached, contacting said functionalized surface to an electrolytic solution having a neutral pH in a flow cell and measuring a first impedance value within said electrolytic solution, and then adding a chemical to the electrolytic solution which is able to achieve denaturation of the target DNA and/or RNA. The method further comprises measuring a second impedance value within the flow cell after completion of the denaturation of the DNA and/or RNA target, and then obtaining a value representative for the impact of the chemical on the impedance of the electrolytic solution.

Abstract: A method for monitoring a select analyte in a sample in an electrochemical system. The method includes applying to the electrochemical system a time-varying potential superimposed on a DC potential to generate a signal; and discerning from the signal a contribution from the select analyte by resolving an estimation equation based on a Faradaic signal component and a nonfaradaic signal component.

Abstract: Molecularly imprinted conducting polymer (MICP) films were electro-polymerized on glassy carbon electrode having specific recognition sites for amino acid viz. L-tyrosine and/or L-cysteine. The amino acid templates in various imprinted films were ejected out by over-oxidation followed by washing and stabilization. Again, the template leached MICP films were modified with metal oxides [oxides of Cu2+, Hg2+, Pd2+, Au3+, Pt4+ etc]. The resultant leached MICP and metal oxide modified template leached MICP film based GCE will now selectively sense L-tyrosine and/or L-cysteine in aqueous media by direct and catalytic means respectively employing differential pulse voltammetric waveform. The sensitivity and selectivity of the sensors prepared by the invention are high and the stability is good, which will be widely used in clinical diagnostics, chemical industry, environment protection and other related fields.

Abstract: A method of forming a biosensor electrode for HER2 detection is provided. The method includes providing a metal electrode, forming a metal electrode/3-mercaptopropionic acid (MPA) by depositing MPA on the metal electrode, forming a metal electrode/MPA/n-hydroxy-succinimide (NHS) by ester-bonding between NHS and the MPA on the metal electrode/MPA, and forming a metal electrode/MPA/aptamer by amide-bonding between an HER2 specific aptamer having an amino terminus and the MPA of the metal electrode/MPA/NHS.

Abstract: A technique for a nanodevice is provided. A reservoir is separated into two parts by a membrane. A nanopore is formed through the membrane, and the nanopore connects the two parts of the reservoir. The nanopore and the two parts of the reservoir are filled with ionic buffer. The membrane includes a graphene layer or a graphene oxide layer. The nanopore could be oxidized to graphene oxide at an inner surface. The graphene or graphene oxide in the nanopore is coated with an organic layer configured to interact with biomolecules in a different way in order to differentiate the biomolecules. The organic layer enhances resolution and motion control of the biomolecules. A time trace of ionic current is monitored to identify the biomolecules based on a respective interaction of the biomolecules with the organic layer.

Abstract: The present invention relates to systems, methods, and devices for determining the concentration of an analyte in a sample. The use of linear, cyclic, or acyclic voltammetric scans and/or semi-integral, derivative, or semi-derivative data treatment may provide for increased accuracy when determining the concentration of an analyte in a sample. Hematocrit compensation in combination with the data treatments may reduce the hematocrit effect with regard to a glucose analysis in whole blood. In another aspect, fast scan rates may reduce the hematocrit effect.

Abstract: A method of measuring a glycosylated protein proportion by an AC impedance method includes passing an AC voltage or AC to two inspecting electrodes; covering a solution containing glycosylated protein completely onto the inspecting electrodes; measuring an impedance value between the electrodes and calculating the difference between the impedance value and an impedance value of a solution without glycosylated protein by an AC impedance inspection device; comparing the impedance difference with AC impedance difference/glycosylated protein concentration to obtain the proportion of the glycosylated protein in the solution containing glycosylated protein.

Abstract: An amperometric electrochemical sensor configured to be operable in an oxidizing atmosphere and under an applied bias to exhibit enhanced reduction of oxygen molecules at the sensing electrode in the presence of one or more target gas species and a resulting measurable increase in oxygen ion flux through the cell. The sensor has an electrolyte membrane, a sensing electrode on the electrolyte membrane, and a counter electrode on the electrolyte membrane, wherein the sensing electrode includes at least one molybdate or tungstate compound. An electrochemical sensor system is also provided, along with a method of detecting the concentration of one or more of NOx and NH3 in a gas sample or stream.

Abstract: A method for detecting at least one property of a gas in a measuring gas chamber, in particular for detecting at least one gas component of the gas. The at least one property is determined using at least one electrochemical measuring cell of a sensor element. Temperatures are detected at at least two different locations of the sensor element and used in determining the at least one property.

Abstract: A fluid sample is measured with a tear film measuring system that includes a processing device that receives a sample chip comprising a sample region configured to contain an aliquot volume of sample fluid, the processing device configured to perform analyses of osmolarity and of one or more biomarkers within the sample fluid, wherein the analysis of biomarkers includes normalization of biomarker concentration values.

Abstract: A NOx sensor includes a sensor element equipped with first and second pumping cells to define first and second measurement chambers. The first pumping cell exerts an oxygen pumping action against the first measurement chamber to adjust the oxygen concentration in the gas under measurement within the first measurement chamber to a given level. The second pumping cell exerts an oxygen pumping action against the second measurement chamber to produce a pumping cell current according to the NOx concentration in the gas under measurement. When the moisture content of the gas under measurement changes from 2 vol % to 8 vol %, the NOx sensor allows a variation of NOx concentration detection value based on the pumping cell current in such a manner that the NOx concentration detection value reaches a transient peak value of 20 ppm or smaller and converges to ±5 ppm of a reference value within 5 seconds.

Abstract: The invention relates to integrated electronic chip (lEChip) cartridge devices which are used in hot electron-induced electrochemiluminescence methods and to instrumentation based on the electrical excitation of label molecules with subsequent measurement of luminescence in order to quantitate analyte concentrations especially in bioaffinity assays, especially outside of central laboratories.

Abstract: Electrochemiluminescent technique and device suitable for cheap analytical and diagnostic applications, with electrodes manufactured from carbon paste and terbium chelates as labeling compounds.

Abstract: The invention describes novel chemiluminescence electrode devices and their novel properties to enable achieving luminescence signal by electrical excitation by cathodic or bipolar pulses in aqueous electrolyte solutions. These devices form a significant improvement in construction of cheap and reliable means for especially diagnosis of health conditions in point-of-need purposes.

Abstract: A cost-effective multi-channel capillary gel-electrophoresis system for highly efficient, nucleic acid-based analysis, in particular HLA SSP (Human Leukocyte Antigen Sequence Specific Primer) typing applications. Twelve DNA samples are automatically injected, electrophoretically separated, detected and analyzed simultaneously by using a multiple usage and disposable multi-capillary gel-cartridge. Using commercially available DNA size markers as indicators, the system provides high resolving power with 12-channel separations in less than 1.5 minutes. The system can hold a total of 96 samples in a PCR plate that can automatically be analyzed within 25 minutes for a full plate of the HLA SSP kits.

Abstract: Provided are a method for measuring hydrofluoric acid content in a lithium secondary battery electrolyte and an analytical reagent composition used in the same.

Abstract: An apparatus is disclosed comprising: a sample conduit; an electrochemical flow cell; a source of carrier fluid; a structure elucidation spectrometer; a conduit and an in-line sample reservoir, such that: (a) a first operating modus wherein the sample conduit is connected to the conduit via the electrochemical flow cell and the in-line sample reservoir, and the source of carrier fluid is connected to the by-passing the in-line sample reservoir, and (b) a second operating modus wherein the sample conduit is connected to the conduit by-passing the in-line sample reservoir, and the source of carrier fluid is connected to the structure elucidation spectrometer via the in-line sample reservoir. Decoupling of the electrochemical cell from the spectrometer allows for uncompromised selection of optimal electrochemical and separation conditions.

Abstract: The method for detection of cyanide in water is a method for the detection of a highly toxic pollutant, cyanide, in water using ZnO2 nanoparticles synthesized locally by an elegant Pulsed Laser Ablation technique. ZnO2 nanoparticles having a median size of 4 nm are synthesized from pure zinc metal target under UV laser irradiation in a 1-10% H2O2 environment in deionized water. The synthesized ZnO2 nanoparticles are suspended in dimethyl formamide in the presence of Nafion, and then ultrasonicated to create a homogenous suspension, which is used to prepare a thin film of ZnO2 nanoparticles on a metal electrode. The electrode is used for cyanide detection.

Abstract: Techniques and systems are disclosed for implementing textile-based screen-printed amperometric or potentiometric sensors. The chemical sensor can include carbon based electrodes to detect at least one of NADH, hydrogen peroxide, potassium ferrocyanide, TNT or DNT, in liquid or vapor phase. In one application, underwater presence of chemicals such as heavy metals and explosives is detected using the textile-based sensors.

Abstract: Novel catalytic materials and methods for producing the same are shown and described. The present disclosure provides catalytic materials formed from producing an alloy of an oxophilic metal and a metal having electro-oxidative activity using spray pyrolysis. The present disclosure further provides methods and mechanisms for both detecting and removing hydrazine from a system.

Abstract: A porous electrochemical electrode is made up of a solid cellular material provided in the form of a semi-graphitised carbon monolith comprising a hierarchised porous network free of mesopores and including macropores with a mean dimension dA of 1 ?m to 100 ?m, and micropores with a mean dimension dI of 0.5 nm to 2 nm, said macropores and micropores being interconnected. In said electrode, the macropores contain at least one electroactive species in direct contact with the semi-graphitised carbon that makes up the surface of the macropores. The invention also relates to a method for preparing such an electrode as well as to the use thereof as a biosensor or for manufacturing a biopile.

Abstract: According to the present invention, phenols may be detected using an electrochemical sensor comprising a first compound, a working electrode and an electrolyte in contact with the working electrode, wherein the first compound operatively undergoes a redox reaction at the working electrode to form a second compound which operatively reacts in situ with the phenol, wherein said redox reaction has a detectable redox couple and wherein the sensor is adapted to determine the electrochemical response of the working electrode to the consumption of said second compound on reaction with the phenol. The phenol may be, for example, a cannabinoid or a catechin compound.

Abstract: The disclosure also relates to novel compositions and methods for the single, direct detection of Hemoglobin A1c percentage in a sample, using conversion of functional groups attached to a transitional metal complex, resulting in quantifiable electrochemical signal at two unique potentials, Eo1 and Eo2.

Abstract: A gas sensor including a detection element having a stacked structure configured to detect a specified gas component contained in a gas to be detected. The detection element includes: a sensing portion including one or more solid electrolyte layers containing a first material as a main component and having a first surface and a second surface opposite the first surface; a first portion stacked on the first surface and including one or more first base layers containing a second material as a main component different from the first material; and a second portion stacked on the second surface and including one or more second base layers containing the second material. A total thickness of the one or more second base layers in a stacking direction is not less than 80% but not more than 120% of a total thickness of the one or more first base layers.

Abstract: Apparatus and methods are provided for improving sensitivity, throughput, and efficiency of multi-analyte analytical testing. Specifically, an improved Electrochemiluminescence (ECL) analytical apparatus is provided for analytical chemistry, diagnostics, and environmental applications. The ECL apparatus comprises a 96 or more-well plate, where a microarray of working electrodes is placed in each well for high throughput and multi-analyte testing. The microarray of working electrodes connects with a counter electrode forming a two-electrode electrochemical system. Each well is electrically addressable, thereby controlling ECL reactions in flexible modes. The ECL apparatus further comprises a detector of ECL signals, and the detector employs a CCD-chip assembling matrix. Also provided are methods for high-throughput multi-analyte testing.

Abstract: A DNA/RNA detection technology is provided. The open flow detection technique includes a substrate defining a pair of opposing microchannels, a pair of opposing electrodes in the opposing microchannels, and at least one ion exchanging nanomembrane coupled between the opposing microchannels such that the opposing microchannels are connected to each other only through the nanomembrane, wherein the nanomembrane is functionalized with a probe complementary to the macromolecule.

Abstract: A disposable biosensor for determining the content of hemoglobin and hematocrit in a sample of whole blood that includes a laminated strip with a first and second end, at least a reference, a working electrode and a blank electrode embedded in the laminated strip. The working electrode contains a reagent sensitive to hemoglobin or hemotocrit. The blank electrode is used to measure oxidizable species in the fluid sample and to correct the current signal of the working electrode. The construction of the biosensor allows accurate measurement of the impedance of a small fluid sample, which is used to further correct the current signal of the working electrode.

Abstract: The invention relates to a method of detecting a structural change in a molecule, said molecule being attached to a surface, said surface being electrically conductive, wherein the phase of the electrochemical impedance at said surface is monitored, and wherein a change in the phase in the electrochemical impedance at said surface indicates a change in the structure of said molecule. The invention further relates to methods for making arrays having molecules such as, polypeptides attached to electrically conductive surfaces such as electrodes, and to arrays.

Abstract: A method using an electrolytic cell to electrolyze urea to produce at least one of H2 and NH3 is described. An electrolytic cell having a cathode with a first conducting component, an anode with a second conducting component, urea and an alkaline electrolyte composition in electrical communication with the anode and the cathode is used to electrolyze urea. The alkaline electrolyte composition has a hydroxide concentration of at least 0.01 M.

Abstract: Provided are devices and methods for performing multi-point analysis on biological materials, such as cells. In one embodiment, the devices simultaneously collect information related to a characteristic of a cell membrane and a characteristic of the interior of the cell.

Abstract: An electrochemical paper-immunosensor and method for detecting aminoglycoside antibiotics is developed in the present invention. Single-walled carbon nanotubes are coated on the common filtration paper by dip-dry cycles. With antibody against neomycin adding to the coating solution, a high sensitive biosensor for specific detection of neomycin is prepared, satisfying to the rigid authority regulations. The sensor is not only sensitive but also rapid, comparing with the classic ELISA method, with LOD of 0.04 ng mL?1 and the whole detection process lasting less than 30 min. Another notable advantage of this invention is the versatility of the sensor, similar method is engaged to prepare the versatile sensor for other aminoglycoside antibiotics, replacement with relevant antibodies.

Abstract: A layered electrocatalyst for oxidizing ammonia, ethanol, or combinations thereof, comprising: a carbon support integrated with a conductive metal; at least one first metal plating layer at least partially deposited on the carbon support, wherein the at least one first metal plating layer is active to OH adsorption and inactive to a target species, and wherein the at least one first metal plating layer has a thickness ranging from 10 nanometers to 10 microns; and at least one second metal plating layer at least partially deposited on the at least one first metal plating layer, wherein the at least one second metal plating layer is active to the target species, and wherein the at least one second metal plating layer has a thickness ranging from 10 nanometers to 10 microns, forming a layered electrocatalyst.

Abstract: A galvanic sensor for analyzing gases present in blood includes a duct suitable for being crossed by a flow of gas and provided with an inlet opening and an outlet opening, a reference galvanic element including a container containing an electrolytic solution in which a reference electrode is inserted, and a measuring galvanic element. The container is fixed to the duct and the measuring galvanic element includes a measuring electrode arranged transversally to the axis of the duct and a filiform element having a high capillarity so as to act as a wick. The filiform element is anchored to the container and has a first end contacting the measuring electrode and a second end contacting the electrolytic solution. The measuring element of the galvanic sensor is extremely miniaturized and allows to detect in real time and continuously gases in traces, on the order of parts per million or even lower.

Abstract: Disclosed in certain embodiments is a method of determining the individual concentration of different antioxidants of the same class in a sample comprising contacting the sample with an effective amount of phenol; and analyzing the sample by voltammetry.

Abstract: Compositions and methods for nucleic acid sequencing include template constructs that comprise double stranded portions in a partially or completely contiguous constructs, to provide for redundant sequence determination through one or both of sequencing sense and antisense strands, and iteratively sequencing the entire construct multiple times. Additional sequence components are also optionally included within such template constructs. Methods are also provided for the use and preparation of these constructs as well as sequencing compositions for their application.

Abstract: A gas sensor for measuring NOx concentration in measurement gas based on sensor output depending on an amount of the detected oxygen includes an oxygen concentration control part for controlling oxygen concentration in the measurement gas. The oxygen concentration control part allows a control of oxygen concentration in the measurement gas to set at a designated value even after being mounted on an automobile, so that dependency of the sensor output with respect to oxygen concentration can be quantitatively detected. The sensor output for detecting NOx concentration is corrected by estimating an amount of a change accompanying the sensitivity degradation of the sensor output with respect to NOx concentration on the basis of a difference between oxygen output characteristics of the sensor output of an actually-used sensor and oxygen output characteristics of a sensor in the initial state, and correcting the sensor output in accordance with the amount of the change.

Abstract: In order to provide a method for electrochemically detecting an analyte which can detect an analyte with high detection sensitivity, when detecting an analyte S trapped on a working electrode, a label binding substance 90 in which a labeling substance 93 and a first binding substance 92 which traps the analyte S are at least retained on a support 91 composed of polypeptide is brought into contact with the analyte. Alternatively, a complex containing the analyte S and a label binding substance 290 in which a labeling substance 293 is retained on a binding substance 291 which binds to the analyte S via a modulator 292 is formed. Then, the labeling substance present on the working electrode is electrochemically detected.

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

Abstract: An ammonia gas sensor is disclosed that includes a reference electrode, an ammonia selective sensing electrode and an electrolyte disposed therebetween. The ammonia sensing electrode comprises an oxide material characterized by the formula CewAxLyVOz wherein L is lanthanum or another lanthanide element other than cerium, A is one or more other metals, and w, x, y and z are numbers indicative of atomic proportion with w ranging from about 0.001 to about 4 x ranging from 0 to about 0.8, y ranging from about 0.001 to about 4, and z with a range to balance the existence of Cew, Ax, Ly and V.

Abstract: An ammonia gas sensor is disclosed that includes a reference electrode, an ammonia selective sensing electrode and an electrolyte disposed therebetween. The ammonia sensing electrode comprises vanadium silicide, vanadium oxysilicide, vanadium carbide, vanadium oxycarbide, vanadium nitride, or vanadium oxynitride.

Abstract: The present invention relates to a method for proportioning nitrate and/or nitrite ions in a solution using a copper electrode, said method being characterized in that it is carried out in constant potential mode and moreover in that it includes the steps of: i. applying a first potential to the copper electrode so as to reduce the copper oxides present on the surface of the metal copper electrode; ii. applying a second potential to the copper electrode so as to oxidize the metal copper formed in Step i into cupric ions; iii. applying a third potential to the copper electrode so as to reduce the copper oxides possibly formed in Step ii. Steps i through iii being carried out in a support electrolyte; and iv.

Abstract: The invention provides an electrochemical sensor comprising an electrode assembly which comprises at least two electrodes, one of the electrodes comprising a metal species capable of catalysing the oxidation of hydrogen and/or methane. The sensor may be used in the detection and quantification of hydrogen and/or methane in exhaled breath, for example as a means of diagnosing lactose malabsorption or lactose intolerance.

Abstract: Microelectrodes, microelectrode formation, and methods of utilizing microelectrodes for characterizing properties of localized environments and substrates are provided. A microelectrode can include a tungsten wire comprising a shaft and a conical tip. The conical tip can include an electroactive area. Further, the microelectrode can include an electroactive coating layer covering one or more surface of the tungsten wire. The tungsten wire surfaces can include a surface of the conical tip. An insulating layer can at least partially cover the shaft.

Abstract: A sample substance S is captured by a capture substance 10 which is immobilized on a working electrode body 161 to provide a method of electrochemically detecting a sample substance capable of detecting the sample substance with high sensitivity. A complex which includes, on a soluble carrier 21, a sample substance S and a labeled binder 20 that has a modified labeled substance 23 containing a labeled substance 20 bound to modified labeled substance 23 containing a labeled substance 24 and a binder 22 that binds to the sample substance S, formed on the working electrode 161. The soluble carrier 21 is dissolved, and the modified labeled substance 23 is attracted to the working electrode 161.