Liquid/liquid Or Gel Type (i.e., Jellylike) Chromatography Patents (Class 210/635)
  • Patent number: 11872564
    Abstract: A thermostatic apparatus thermostatically holds a sample container holding a sample. The thermostatic apparatus includes a sample rack which accommodates and holds a plurality of the sample containers and is attachable to and detachable from the thermostatic apparatus; and a heat conduction member which is controlled to a constant temperature and transfers heat to the sample container, in which an opening portion is formed in the sample rack, and when the sample rack is mounted on the thermostatic apparatus, a contact portion forming one part of the heat conduction member directly contacts the sample container by passing through the opening portion, or directly contacts the sample container protruding from the opening portion.
    Type: Grant
    Filed: August 21, 2018
    Date of Patent: January 16, 2024
    Assignee: Hitachi High-Tech Science Corporation
    Inventors: Atsuki Morisaki, Koji Yamamoto, Masahito Ito
  • Patent number: 11835501
    Abstract: Determining optimum operating binding capacity for a MCC process using one column, the process including N number of columns, comprises loading target product on a first column at a first residence time and/or flow rate; loading the product on the column at a second residence time and/or flow rate, the first residence time and/or flow rate being different than the second residence time and/or flow rate; generating a first breakthrough curve for the first residence time and/or flow rate and a second breakthrough curve for the second residence time and/or flow rate, wherein the first curve represents product breakthrough for the first column and the second curve represents product breakthrough for an Nth column; and using the curves to determine product loading capacity of the first column before product breakthrough at the Nth column; the product loading capacity of the first column equaling optimum operating binding capacity for the process.
    Type: Grant
    Filed: July 13, 2015
    Date of Patent: December 5, 2023
    Assignee: Sartorius Stedim Chromatography Systems Ltd.
    Inventors: Xhorxhi Gjoka, Karl K. Rogler, Mark J. Schofield
  • Patent number: 11827990
    Abstract: A dual-membrane on-line generator for an acid or alkali solution is provided, including an upper electrolytic cell body (3), a middle electrolytic cell body (4) and a lower electrolytic cell body (5) which are clamped by an upper fastening steel plate (1) and a lower fastening steel plate (2), an upper regeneration liquid channel (A), a middle eluent channel (B) and a lower regeneration liquid channel (C) being provided on the middle electrolytic cell body (4).
    Type: Grant
    Filed: June 14, 2019
    Date of Patent: November 28, 2023
    Assignees: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY, SHIMADZU (CHINA) CO., LTD.
    Inventors: Bingcheng Yang, Feifang Zhang, Yifei Lu, Liting Zhou, Shujun Huang
  • Patent number: 11511257
    Abstract: In embodiments, a packing material for supported liquid extraction has a sorbent media that includes synthetic silica particles. In embodiments, the synthetic silica particles can have physical properties relating to one or more of particle surface area, shape, size, or porosity. In one embodiment, synthetic silica particles have a surface area less than about 30 m2/g. In another embodiment, the synthetic silica particles have an approximately uniform particle shape. In further examples, synthetic silica particles have a particle size in a range of about 30-150 ?m inclusive or greater than about 200 ?m. In another embodiment, synthetic silica particles are arranged to have a pore size greater than about 500 Angstroms. In an embodiment, an apparatus for supported liquid extraction includes a container and a sorbent media that includes synthetic silica particles. In a further embodiment, a method for extracting target analytes through supported liquid extraction is provided.
    Type: Grant
    Filed: April 9, 2020
    Date of Patent: November 29, 2022
    Assignee: AGILENT TECHNOLOGIES, INC.
    Inventors: Kunqiang Jiang, Derick Lucas, Bruce Richter
  • Patent number: 11426706
    Abstract: A wash buffer comprising a surfactant for use in affinity and cation exchange chromatography to purify proteins of interest from protein aggregates and to remove and/or inactivate viruses. When used during affinity or cation exchange chromatography for the purification of a protein of interest, such as an antibody, the wash buffer significantly improves viral clearance from the preparation, while also reducing the levels of host cell proteins and protein aggregates. Following affinity or cation exchange chromatography with the wash buffer, the protein of interest may be further purified using other chromatography and filtration operations.
    Type: Grant
    Filed: June 21, 2018
    Date of Patent: August 30, 2022
    Assignee: Cephalon, Inc.
    Inventors: Lu Wang, Albert Kao, Zhaoqing Zhang, Mi Jin, Tianyi Zhou
  • Patent number: 11320864
    Abstract: A flexible cover plate, a flexible display apparatus, and a flexible terminal product are provided to make the flexible cover plate feature a repeated bending capability and sufficient rigidity. The flexible cover plate includes a first cover-plate layer, a second cover-plate layer, and a flow layer. The flow layer is enclosed between the first cover-plate layer and the second cover-plate layer by using a transparent sealing material. The first cover-plate layer and the second cover-plate layer each have a thickness ranging from 0.05 mm to 0.5 mm, and the flow layer has a thickness ranging from 0.1 mm to 0.2 mm. At least one first support bump is attached to an inner side of the first cover-plate layer, and at least one second support bump is attached to an inner side of the second cover-plate layer.
    Type: Grant
    Filed: May 14, 2020
    Date of Patent: May 3, 2022
    Assignee: Huawei Technologies Co., Ltd.
    Inventors: Zhaoliang Su, Wangchun Lyu, Wenming Shi
  • Patent number: 11248076
    Abstract: The present invention relates to a homopolymer, copolymer, block copolymer, and statistical copolymer comprising plural polyol monomeric units. The polyol monomeric units being acrylated and acylated or acetalized. The acrylated and acylated or acetalized polyol monomeric units have an average degree of acrylation which is 1 or more, but less than the number of the hydroxyl groups of the polyol and have an average degree of acylation or acetalization which is 1 or more, but less than the number of the hydroxyl groups of the polyol. The present invention also relates to a method of making the homopolymers, copolymers, block copolymers, and statistical copolymers, and using them in various applications, such as asphalt rubber modifiers, adhesives, or an additive in a fracking fluid for oil fracking.
    Type: Grant
    Filed: February 11, 2019
    Date of Patent: February 15, 2022
    Assignee: IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.
    Inventors: Eric W. Cochran, Nacu Hernandez, Michael Forrester, Shailja Goyal, Austin Hohmann
  • Patent number: 11243194
    Abstract: A suppressor for use in reducing a background signal while detecting analytes in a liquid sample for ion chromatography is described. The suppressor includes a central channel and two flanking regenerant channels. The central channel is formed with an eluent channel plate including a central cutout portion having a peripheral boundary portion. The peripheral boundary portion includes a recessed eluent plate height that is less than the eluent plate height. The peripheral boundary portion includes two or more notches or protrusions. An eluent screen is disposed in the central cutout portion, in which a peripheral border of the eluent screen has two or more corresponding notches or corresponding protrusions to the eluent channel plate.
    Type: Grant
    Filed: January 25, 2019
    Date of Patent: February 8, 2022
    Assignee: Dionex Corporation
    Inventors: Kannan Srinivasan, Sheetal Bhardwaj, Glenn M. Kuse
  • Patent number: 11237138
    Abstract: The present disclosure is directed at the selection and design of columns for liquid chromatography including liquid chromatography devices and systems and corresponding methods of operation, particularly in the field of high pressure liquid chromatography (HPLC).
    Type: Grant
    Filed: June 19, 2019
    Date of Patent: February 1, 2022
    Assignee: THE WRANGLER GROUP
    Inventor: Hubert M. Quinn
  • Patent number: 11168110
    Abstract: Herein is reported a method for obtaining a polypeptide in monomeric form comprising the steps of a) providing a solution comprising the polypeptide in monomeric form and in aggregated form, wherein the ratio of monomeric to aggregated form is 4:1 or less as determined by size exclusion chromatography, b) performing a mixed-mode chromatography in bind-and-elute mode, or a hydrophobic interaction chromatography in flow-through mode, or a size-exclusion chromatography, and c) performing a weak cation exchange chromatography in bind-and-elute mode or flow-through mode, and thereby obtaining the polypeptide in monomeric form.
    Type: Grant
    Filed: April 17, 2020
    Date of Patent: November 9, 2021
    Assignee: HOFFMANN-LA ROCHE INC.
    Inventors: Monika Baehner, Adelbert Grossmann, Stefan Hepbildikler
  • Patent number: 11143634
    Abstract: Methodologies, systems, and computer-readable media are provided for controlling the mass flow rate within a CO2 based chromatography system. The pressure within a CO2 pump is measured and received at a computing system, and the computing system retrieves a target temperature value corresponding to the new pressure measurement within the CO2 pump. The computing system then generates a temperature control command that controls a CO2 pump heater or cooler in order to achieve the target temperature value at the CO2 pump. Thus, a target mass flow rate of CO2 from the CO2 pump is achieved by adjusting the temperature of the CO2 pump in response to changes in pressure.
    Type: Grant
    Filed: October 26, 2018
    Date of Patent: October 12, 2021
    Assignee: Waters Technologies Corporation
    Inventors: Abhijit Tarafder, Jason Hill, Joshua Shreve
  • Patent number: 11021588
    Abstract: The present invention provides a technique for preparing a porous cellulose medium, through the gelation of a solution in which cellulose acetate as a raw material is dissolved, which does not cause a reaction involving the migration of substances. Specifically, a method for producing a porous cellulose medium is provided which includes the steps of gelling a flowable homogeneous composition containing cellulose acetate, an organic solvent, and water through a decrease in temperature; and hydrolyzing the cellulose acetate contained in the resulting gel.
    Type: Grant
    Filed: July 22, 2015
    Date of Patent: June 1, 2021
    Assignee: DAICEL CORPORATION
    Inventors: Toru Shibata, Yasuto Morishita, Yosuke Ito
  • Patent number: 10954550
    Abstract: Provided are a method for screening for nanobodies and a corresponding system. The method uses polymerase chain reactions and cDNA 5? end rapid amplification technology to screen for and obtain nanobodies. The experiment cycle requires only approximately 21 days.
    Type: Grant
    Filed: August 6, 2015
    Date of Patent: March 23, 2021
    Assignee: BGI SHENZHEN
    Inventors: Xiao Liu, Chao Nie, Wei Zhang, Zhe Ren, Ruifang Zhang, Xiaojing Zeng, Xinyang Li, Naibo Yang
  • Patent number: 10934366
    Abstract: Methods and kits for purifying protein-nanoparticle conjugates are provided. In some embodiments, a multimodal medium having a size exclusion mode and a capture mode is used to purify the protein-nanoparticle conjugates.
    Type: Grant
    Filed: September 25, 2017
    Date of Patent: March 2, 2021
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Jiali Liao, Tom Berkelman
  • Patent number: 10858596
    Abstract: A method and a system for separating and recovering an entire liquid hydrocarbon sample using an accelerated solvent extractor is disclosed. In the method, a filter is inserted into a bottom portion of an extraction cell of the accelerated solvent extractor. An adsorbent is activated via heating in a furnace and then cooled. At least a portion of the adsorbent is then inserted into the extraction cell and a liquid hydrocarbon sample is introduced into the extraction cell on top of the adsorbent. The extraction cell comprising the sample is placed in a cell tray of the accelerated solvent extractor and the saturate, aromatics, and resins fractions of the sample are sequentially extracted using first, second and third solvents, respectively. The entire liquid hydrocarbon sample is extracted as a result of the method.
    Type: Grant
    Filed: March 6, 2019
    Date of Patent: December 8, 2020
    Assignee: Saudi Arabian Oil Company
    Inventors: Frederick M. Adam, Zahra N. Almisbaa, Mansour S. Alzayer, Faisal Alrasheed
  • Patent number: 10745660
    Abstract: A system for incubating microfluidic droplets includes a droplet incubator, a gas exchanger and a conveyor. A method provides homogeneous incubation conditions in a droplet incubator by targeted introduction of gases and/or gas mixtures into a carrier fluid.
    Type: Grant
    Filed: November 10, 2015
    Date of Patent: August 18, 2020
    Assignee: LEIBNIZ-INSTITUT FUR NATURSTOFF-FORSCHUNG UND INFEKTIONSBIOLOGIE—HANS-KNÖLL-INSTITUT
    Inventors: Miguel Tovar, Lisa Mahler, Emerson Zang, Martin Roth
  • Patent number: 10718743
    Abstract: A liquid chromatography (LC) column includes a wall having a length along a central axis from the inlet end to the outlet end, the wall enclosing a column interior and having a column radius relative to the central axis, the wall comprising a structured portion configured such that the column radius varies along the length; and a plurality of particles packed in the column interior, wherein at least some of the particles are in contact with the structured portion.
    Type: Grant
    Filed: August 2, 2018
    Date of Patent: July 21, 2020
    Assignee: Agilent Technologies, Inc.
    Inventors: Hongfeng Yin, Reid A. Brennen, Eric Lyster, Roger Slocum
  • Patent number: 10676503
    Abstract: A method for purifying a protein comprising an antibody, antibody fragment, or immunoglobulin single variable domain, from a solution containing at least one contaminant by superantigen chromatography comprising: a) adsorbing the protein to the superantigen immobilized on a solid support; b) removing the at least one contaminant by contacting the immobilized superantigen containing the adsorbed protein with a first wash buffer comprising an aliphatic carboxylate; and c) eluting the protein from the superantigen immobilized on the solid support.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: June 9, 2020
    Assignee: GLAXOSMITHKLINE INTELLECTUAL PROPERTY (NO.2) LIMITED
    Inventors: Kent E. Goklen, Eric J. Suda, Antonio Raul Ubiera
  • Patent number: 10569190
    Abstract: Provided is a packed column capable of achieving sufficiently high distillation performance even with the height of its gas-liquid contactors reduced. The packed column is a packed column which includes a gas-liquid contactor 17, 18 inside a tubular body 16 and a liquid distributor 19 in the upper most portion and causes descending liquid and ascending gas to contact each other in the gas-liquid contactor. The operation pressure is in the range of 200 to 1500 kPaG. The relative volatility is in the range of 1.9 to 3.1. The gas-liquid contactor is vertically divided into at least two parts. A gas disperser 20 is provided at at least one position between a lower one of the gas-liquid contactors and an upper one of the gas-liquid contactors, the gas disperser uniformly dispersing the composition of the ascending gas rising from the lower gas-liquid contactor toward the upper gas-liquid contactor.
    Type: Grant
    Filed: March 3, 2017
    Date of Patent: February 25, 2020
    Assignee: TAIYO NIPPON SANSO CORPORATION
    Inventors: Takeyuki Ito, Nobuaki Egoshi
  • Patent number: 10571452
    Abstract: A system and a method are provided for calculating one or more indicative properties, e.g., one or more of the cetane number, octane number, pour point, cloud point and aniline point of oil fractions, from the density and high pressure liquid chromatography (HPLC) data of a sample of the crude oil.
    Type: Grant
    Filed: June 30, 2017
    Date of Patent: February 25, 2020
    Assignee: Saudi Arabian Oil Company
    Inventors: Omer Refa Koseoglu, Adnan Al-Hajji, Saroj Kumar Panda
  • Patent number: 10487138
    Abstract: The present invention relates to the purification of immunoglobulins and the problem of providing a method for purifying an immunoglobulin in an efficient and cost-effective manner and with satisfactory purity and yield. In particular, the present invention addresses the aspect of the re-use of the rather cost-intensive chromatography materials, in particular the lifetime of the chromatography materials used in the capture step of the downstream process, and how this can be increased while reducing the technical complexity of the purification process.
    Type: Grant
    Filed: March 9, 2015
    Date of Patent: November 26, 2019
    Assignee: RICHTER GEDEON NYRT.
    Inventors: Ferenc Felföldi, Zsuzsa Benkö, Melinda Gáspár
  • Patent number: 10444216
    Abstract: A hand-held microfluidic testing device is provided that includes a housing having a cartridge receiving port and a cartridge for input to the cartridge receiving port. An optical detection system in the housing is capable of providing an illuminated electric field useful for Raman spectroscopy. The cartridge may have a sample well. The sample well is loaded with a mixture of water containing the analyte, Raman-scattering nanoparticles and a calibration solution. The calibration solution contains an analog of the analyte differing in its Raman response, for example an isotope of the analyte. Optionally, a chemical compound capable of increasing interaction between the analyte and the nanoparticles may be added.
    Type: Grant
    Filed: November 22, 2017
    Date of Patent: October 15, 2019
    Assignee: ONDAVIA, INC.
    Inventors: Mark Charles Peterman, Merwan Benhabib, Carlos Atico Ariza, Samuel Louis Kleinman
  • Patent number: 10408811
    Abstract: A crude oil composition estimation method estimates a composition of crude oil for use in simulation of a material balance in an absorption and liquefaction system that absorbs vapors emitted from the crude oil with the crude oil. The crude oil composition estimation method includes: comparing a calculated concentration of a treated gas with a measured concentration of the treated gas; estimating, when the calculated concentration agrees with the measured concentration, that the concentration included in the crude oil is a correct crude oil composition; and repeating, when the calculated concentration does not agree with the measured concentration, a process of correcting the concentration included in the crude oil and then comparing the calculated concentration with the measured concentration, until the calculated concentration agrees with the measured concentration, and when they agree, estimating that the corrected concentration in the crude oil is a correct crude oil composition.
    Type: Grant
    Filed: September 30, 2015
    Date of Patent: September 10, 2019
    Assignee: JFE Engineering Corporation
    Inventors: Yoshiki Shibuya, Kengo Murai
  • Patent number: 10399030
    Abstract: A Chromatography system that includes a microfluidic device configured to provide three-way switching or switching between three or more inputs or outputs. The microfluidic device fluidically coupled to one or more switching valves to provide for selective control of fluid flow in the chromatography system.
    Type: Grant
    Filed: September 4, 2017
    Date of Patent: September 3, 2019
    Assignee: PerkinElmer Health Sciences, Inc.
    Inventor: Andrew Tipler
  • Patent number: 10278405
    Abstract: The present invention concerns compositions and methods relating to the addition and use of par-baked and milled coffee beans in food products, beverages, and dietary supplements. Par-baked and milled coffee beans are a novel alternative to more costly chlorogenic acid extracts, and are edible and palatable as a food ingredient. The ingredient is prepared from the entire Coffea robusta or arabica green coffee bean and provides substantially increased amounts of dietary chlorogenic acid antioxidants compared to roasted beans. When the green coffee bean is appropriately par-baked at a temperature significantly lower than coffee roasting temperatures, the bean retains significant moisture, yet becomes embrittled, enabling milling to produce fine and beneficially hydrophilic particles, without any significant loss of bioactive chlorogenic acid and without significant pyrolysis occurring.
    Type: Grant
    Filed: March 8, 2018
    Date of Patent: May 7, 2019
    Assignee: Brandeis University
    Inventor: Daniel Perlman
  • Patent number: 10252186
    Abstract: In a solid phase extraction (SPE) apparatus, a cartridge housing tray houses a plurality of SPE cartridges. A liquid loading assembly applies liquid samples and SPE solutions into the plurality of cartridges. A liquid drawing assembly forces the liquid samples and the SPE solutions once they are applied to pass through the plurality of the SPE cartridges.
    Type: Grant
    Filed: August 9, 2016
    Date of Patent: April 9, 2019
    Assignee: ZIST ABZAR PAJOOHAN
    Inventor: Seyed Kamal Fatemi
  • Patent number: 10233487
    Abstract: A method of generating deformable gel beads that contain a single colloidal particle is described. Gel beads containing a particle are isolated from those that do not contain a particle, based on differences between the buoyant density of these gel bead populations. The gel beads containing a particle are subsequently co-encapsulated at a high efficiency into droplets that can comprise additional objects such as cells, other particles, or reagents. The gel coating on the rigid particle prevents clogging in narrow channels, such as those of a droplet generator.
    Type: Grant
    Filed: March 23, 2017
    Date of Patent: March 19, 2019
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Andrew Utada, Jeremy Agresti
  • Patent number: 10209377
    Abstract: A method of distinguishing effective pulses from test pulses in a scintillation detector that generates measurement light pulses includes providing a regularly-pulsed test light source that produces individual test light pulses having a time-dependent course of relative light intensity, which differs from a time-dependent course of relative light intensity of the measurement light pulses. The test light pulses are provided to a light detector for measurement of the test light pulses. The time-dependent courses of the relative light intensities of the test light pulses are analyzed. The measured pulses are separated into the test light pulses and the measurement light pulses according to the different time-dependent courses of the relative light intensities. The detector includes a scintillator, a light detector, a regularly-pulsed test light source that is adapted provide test light pulses to the light detector for measurement, and an electronic measuring circuit.
    Type: Grant
    Filed: November 8, 2004
    Date of Patent: February 19, 2019
    Assignee: FLIR Detection, Inc.
    Inventors: Jurgen Stein, Guntram Pausch
  • Patent number: 9951222
    Abstract: An object of the present invention is to provide a method for efficiently and highly selectively preparing a composition comprising perfluoropolyether having a hydroxyl group at one terminal at a higher ratio. The present invention provides a method for increasing a ratio of perfluoropolyether having a hydroxyl group at one terminal, relative to a total amount of the perfluoropolyether having a hydroxyl group at one terminal and perfluoropolyether having hydroxyl groups at both terminals in a composition comprising these perfluoropolyethers, wherein the method comprises a step of subjecting the composition to chromatography in which a moving phase is supercritical or subcritical state carbon dioxide of the specific temperature and the specific pressure, and a stationary phase is silica gel to thereby collect a fraction containing the perfluoropolyether having a hydroxyl group at one terminal at a higher ratio.
    Type: Grant
    Filed: April 9, 2015
    Date of Patent: April 24, 2018
    Assignee: SHIN-ETSU CHEMICAL CO., LTD.
    Inventors: Yuji Yamane, Takashi Matsuda, Noriyuki Koike
  • Patent number: 9950087
    Abstract: A biocidal treatment evaporates a liquid having biocidal properties. An absorption organ has absorbent strips suited to retain the liquid, each absorbent strip having a top first end and a bottom second end, The liquid is injected into the first end of each absorbent strip, the liquid flowing by gravity along the absorbent strip towards the second end, the liquid having biocidal properties being injected at a predetermined flow rate. The liquid is evaporated by producing a gas flow directed towards the absorption organ at a temperature below 50° C.
    Type: Grant
    Filed: October 9, 2015
    Date of Patent: April 24, 2018
    Assignee: XEDA INTERNATIONAL
    Inventor: Alberto Sardo
  • Patent number: 9936717
    Abstract: The present invention concerns compositions and methods relating to the addition and use of par-baked and milled coffee beans in food products, beverages, and dietary supplements. Par-baked and milled coffee beans are a novel alternative to more costly chlorogenic acid extracts, and are edible and palatable as a food ingredient. The ingredient is prepared from the entire Coffea robusta or arabica green coffee bean and provides substantially increased amounts of dietary chlorogenic acid antioxidants compared to roasted beans. When the green coffee bean is appropriately par-baked at a temperature significantly lower than coffee roasting temperatures, the bean retains significant moisture, yet becomes embrittled, enabling milling to produce fine and beneficially hydrophilic particles, without any significant loss of bioactive chlorogenic acid and without significant pyrolysis occurring.
    Type: Grant
    Filed: December 1, 2015
    Date of Patent: April 10, 2018
    Assignee: Brandeis University
    Inventor: Daniel Perlman
  • Patent number: 9938089
    Abstract: An automated positive pressure solid phase extraction apparatus and method comprising two tiered lifts devices each individually controllable to individually vertically translate within an elevator framework between a base on which the elevator framework is mounted and a manifold plate supported by the framework in a substantially horizontal plane parallel with and vertically above the two tiered lifts devices and a rectilinearly translating shuttle assembly comprising a shuttle supporting labware for rectilinear travel into and out of the elevator framework to handoff the labware to one of the two tiered elevator lifts or both.
    Type: Grant
    Filed: January 16, 2015
    Date of Patent: April 10, 2018
    Assignee: Hamilton Company
    Inventors: Garrett Voss, Harrison Elings
  • Patent number: 9925478
    Abstract: Methods for purifying nanocrystals via gel permeation chromatography are provided. The method can include: loading a sample solution into a chromatography column, wherein the sample solution comprises nanocrystals coated with a layer of organic ligands; using an eluent, passing the nanocrystals coated with the layer of organic ligands through a stationary phase of the chromatography column, wherein the eluent is an organic solvent in which the nanocrystals coated with the layer of organic ligands remain miscible to form a mobile phase; and collecting the mobile phase passed through the stationary phase of the chromatography column. The surface ligands of the purified nanocrystals can then be, in one embodiment, replaced with a second type of ligand.
    Type: Grant
    Filed: June 27, 2014
    Date of Patent: March 27, 2018
    Assignee: University of South Carolina
    Inventors: Andrew B. Greytak, Yi Shen
  • Patent number: 9911520
    Abstract: Disclosed herein are monolayers comprising alkylamine-gold nanoparticles that have tunable electrical and optical properties. Also disclosed is a method for forming the monolayers that comprises self-assembly of the nanoparticles.
    Type: Grant
    Filed: October 19, 2016
    Date of Patent: March 6, 2018
    Assignee: FLORIDA STATE UNIVERSITY RESEARCH FOUNDATION, INC.
    Inventors: Daniel Hallinan, Jr., Guang Yang
  • Patent number: 9890205
    Abstract: A method of purifying a sample containing a desired protein includes the steps of (i) providing a packed chromatographic column having positively charged porous particles, (ii) equilibrating the column to the conditions to which the desired protein in the sample is to elute, (iii) contacting the sample with the packed chromatographic column such that the sample volume applied to the packed chromatographic column is less than or equal to the interparticle space of the positively charged porous particles within the packed chromatographic column, (iv) eluting the desired protein from the packed chromatographic column, where the desired protein is in a purer state and in the conditions to which the packed chromatographic column was equilibrated; where the desired protein is an antibody, an antibody fragment, an antibody derivative, or an antibody fusion protein.
    Type: Grant
    Filed: November 26, 2014
    Date of Patent: February 13, 2018
    Assignee: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
    Inventor: Peter Gagnon
  • Patent number: 9879308
    Abstract: Systems and methods for culturing and monitoring, ex vivo, pharmacologic and metabolic response in a biological sample, including receiving at a fluidic apparatus the biological sample retrieved from the patient, retaining the biological sample within a channel of the fluidic apparatus, providing for the culture of the biological sample within the channel of the fluidic apparatus, flowing a fluid past the biological sample, retrieving and analyzing the fluid to determine a pharmacologic and/or metabolic response of the sample.
    Type: Grant
    Filed: December 30, 2014
    Date of Patent: January 30, 2018
    Assignee: The Charles Stark Draper Laboratory, Inc.
    Inventors: Angela Holton, Alla Gimbel, David Landis, Abigail Spencer
  • Patent number: 9863924
    Abstract: A hand-held microfluidic testing device is provided that includes a housing having a cartridge receiving port and a cartridge for input to the cartridge receiving port. An optical detection system in the housing is capable of providing an illuminated electric field useful for Raman spectroscopy. The cartridge may have a sample well. The sample well is loaded with a mixture of water containing the analyte, Raman-scattering nanoparticles and a calibration solution. The calibration solution contains an analog of the analyte differing in its Raman response, for example an isotope of the analyte. Optionally, a chemical compound capable of increasing interaction between the analyte and the nanoparticles may be added.
    Type: Grant
    Filed: September 16, 2016
    Date of Patent: January 9, 2018
    Assignee: OndaVia, Inc.
    Inventors: Mark Charles Peterman, Merwan Benhabib, Carlos Atico Ariza, Samuel Louis Kleinman
  • Patent number: 9827552
    Abstract: A solid phase for use in separation has been modified using an aqueous phase adsorption of a headgroup-modified lipid to generate analyte specific surfaces for use as a stationary phase in separations such as high performance liquid chromatography (HPLC) or solid phase extraction (SPE). The aliphatic moiety of the lipid adsorbs strongly to a hydrophobic solid surface, with the hydrophilic and active headgroups orienting themselves toward the more polar mobile phase, thus allowing for interactions with the desired solutes. The surface modification approach is generally applicable to a diversity of selective immobilization applications such as protein immobilization clinical diagnostics and preparative scale HPLC as demonstrated on capillary-channeled fibers, though the general methodology could be implemented on any hydrophobic solid support material.
    Type: Grant
    Filed: July 17, 2014
    Date of Patent: November 28, 2017
    Assignee: Clemson University
    Inventors: R. Kenneth Marcus, Kenneth A. Christensen
  • Patent number: 9733239
    Abstract: Reconfigurable microfluidic systems are based on networks of microfluidic cavities connected by hydrophobic microfluidic channels. Each cavity is classified as either a reservoir or a node, and includes a pressure port via which gas pressure may be applied. Sequences of gas pressures, applied to reservoirs and nodes according to a fluid transfer rule, enable fluid to be moved from any reservoir to any other reservoir in a system. Systems may be configured with multiple switched interaction regions connected in series for scalable, multiplexed immunoassays. Multiple, switched interaction regions may also be implemented with microvalves.
    Type: Grant
    Filed: July 24, 2015
    Date of Patent: August 15, 2017
    Assignee: HJ Science & Technology, Inc.
    Inventors: Hong Jiao, Erik C Jensen, Homayun Mehrabani, Liran Yosef Haller
  • Patent number: 9663553
    Abstract: The invention is an integrated process for the isolation and purification of plasma derived clotting Factor VIII, clotting Factor IX, albumin and immunoglobulin without the use of ethanol precipitation. The integrated process comprises of sequential chromatography steps of gel filtration, anion exchange and cation exchange chromatographies, followed by viral inactivation and removal steps. The present invention has the advantage of being a mild process that does not denature or aggregate the proteins and provides high yields of several therapeutic grade plasma proteins from a given volume of plasma.
    Type: Grant
    Filed: November 3, 2014
    Date of Patent: May 30, 2017
    Assignee: HEMARUS THERAPEUTICS LIMITED
    Inventors: Neelima Vadde, Mitali Samaddar, Ashok Nuvula, Zinia Chakraborty, Swapna Sagar Duggineni, Uma Devi Komath
  • Patent number: 9631007
    Abstract: A method for the purification of immunoglobulins by ion exchange chromatography is described. The chromatographic method uses a weak ion exchange resin and a single step elution process for the purification of an immunoglobulin. Additionally a method for the determination of the salt concentration for the single step elution of an immunoglobulin from an ion exchange resin is described.
    Type: Grant
    Filed: May 6, 2014
    Date of Patent: April 25, 2017
    Assignee: HOFFMANN-LA-ROCHE INC.
    Inventors: Roberto Falkenstein, Burkard Kolb, Maria Sebald
  • Patent number: 9511575
    Abstract: In one or more embodiments, a porous composite particulate material includes a plurality of composite particles including an acid-base-resistant core particle at least partially surrounded by one or more layers of acid-base-resistant shell particles. The shell particles are adhered to the core particle by a polymeric material. The shell particles and/or core particles may be made from an acid-base-resistant material that is stable in harsh chemical conditions. During application of the polymeric material/shell particle bilayer, the core particles are sonicated to homogenize the particle size distribution and minimize agglomeration of particles. Multiple bilayers of polymer/shell particles may be applied. In one embodiment, the core particle comprises generally spherical glassy carbon, while the shell particles may comprise nano-sized diamond particles. Other acid-base-resistant materials may be employed.
    Type: Grant
    Filed: January 10, 2014
    Date of Patent: December 6, 2016
    Assignee: BRIGHAM YOUNG UNIVERSITY
    Inventors: Matthew R. Linford, Chuan-Hsi Hung
  • Patent number: 9458715
    Abstract: Methods and devices for determining a plus fraction of a plus fraction of a gas chromatogram are provided. A gas chromatogram may obtained, such as from a downhole gas chromatograph module of a fluid analysis tool. The plus fraction of the gas chromatogram may be determined using one or more of a ratiometric determination, fitting an exponential decay function, and fitting a probability density gamma function.
    Type: Grant
    Filed: December 16, 2014
    Date of Patent: October 4, 2016
    Assignee: SCHLUMBERGER TECHNOLOGY CORPORATION
    Inventors: Ronald E. G. van Hal, Jeffrey Crank, Youxiang Zuo, Adriaan Gisolf
  • Patent number: 9417220
    Abstract: Petroleum or other hydrocarbon samples can be analyzed in parallel by 1) GC-field ionization Time of Flight Mass Spectrometer (GC-FI-TOF MS) and 2) two dimensional gas chromatography (2D-GC) equipped with a flame ionization detector (FID). The combined techniques allow for improved quantitative characterization of the compounds within a hydrocarbon sample. The techniques can be combined by correlating the 2D-GC FID data with the GC-FI-TOF MS data based on correlation of compound classes, correlation of retention windows within a compound class, correlation of individual compounds, such as paraffins, or a combination thereof.
    Type: Grant
    Filed: October 23, 2013
    Date of Patent: August 16, 2016
    Assignee: EXXONMOBIL RESEARCH AND ENGINEERING COMPANY
    Inventors: Frank C. Wang, Kuangnan Qian, Kathleen E. Edwards
  • Patent number: 9345989
    Abstract: A drive mechanism for back pressure regulator used in liquid chromatography, supercritical fluid chromatography, or supercritical fluid extraction allows very fine automated control over a very wide range of pressures by combining a linear actuator compressing a spring, pushing a pin. The nozzle assembly of the regulator comprises a flow through chamber containing a diaphragm and a seat, in which the pin pushes the diaphragm against the seat, together with an upstream pressure sensor and electronic feedback control to the motor of the actuator. The BPR of the embodiments exhibits high pressure stability and extremely low pressure noise, even at moderate to high pressures. The exemplary BPR can be use at either constant pressure or to generate pressure programs where the pressure is varied versus time. Further, the nozzle assembly has a field-replaceable head, requiring no mechanical adjustment on replacement.
    Type: Grant
    Filed: April 12, 2013
    Date of Patent: May 24, 2016
    Assignee: Agilent Technologies, Inc.
    Inventors: Terry A. Berger, Samuel Colgate, Michael Casale
  • Patent number: 9340562
    Abstract: A chromatographic material comprising a zwitterionic ligand covalently bound to a substrate, the ligand preferably has a formula II: wherein R1, R2, R3 are independently selected from an oxygen atom that is configured to connect to a substrate atom in the substrate, an oxygen atom that is configured to connect to a silicon atom of an adjacent ligand, a hydroxyl group, a halogen atom, an alkoxy group, a dialkylamino group, an acyl group, an alkyl group, or an aryl group; L1, L2 and L3 are independently hydrophobic moieties; each containing 2 to 30 carbon atoms, wherein there are at least 10 carbon atoms in the combined chain lengths of L1, L2 and L3; X is an O atom, S atom, amide group or sulfonamide group; n is 0 or 1; R4, R5 are independently selected from a hydrogen atom or a hydrocarbon moiety containing 1 to 20 carbon atoms; and Rf is a negatively charged moiety comprising a sulfonic, carboxylic, or phosphonic functional group.
    Type: Grant
    Filed: October 29, 2014
    Date of Patent: May 17, 2016
    Assignees: Thermo Electron Manufacturing Limited, Dionex Corporation
    Inventors: Xiaodong Liu, Richard T. Williams, Xiao Cui
  • Patent number: 9303098
    Abstract: The invention relates to a process for the preparation of a separating material having improved binding capacity, and to materials prepared and to the use thereof for the separation of charged or uncharged biopolymers from liquids.
    Type: Grant
    Filed: May 5, 2009
    Date of Patent: April 5, 2016
    Assignee: MERCK PATENT GMBH
    Inventor: Matthias Joehnck
  • Patent number: 9295928
    Abstract: A porous substrate capable of adsorptive filtration of a fluid having a porous self-supporting substrate and one or more porous, adsorptive polymeric coatings comprising from about 1 to about 80% of the void volume of the pores of the substrate. The resultant substrate has good convective and diffusive flow and capacity. The substrate may be cross-linked, have one or more capture chemistries attached to it and is useful as a chromatography media for the selective filtration of desired species including biomolecules such as proteins and DNA fragments.
    Type: Grant
    Filed: November 2, 2010
    Date of Patent: March 29, 2016
    Assignee: EMD Millipore Corporation
    Inventors: Anthony J. DiLeo, Justin McCue, Wilson Moya, Igor Quinones-Garcia, Neil P. Soice, Volkmar Thom, Sarah Yuan
  • Patent number: 9291630
    Abstract: Disclosed is a method for the separation, identification and quantification of multiple proteins in pancreatin active pharmaceutical ingredient samples.
    Type: Grant
    Filed: August 29, 2012
    Date of Patent: March 22, 2016
    Assignee: Scientific Protein Laboratories, LLC
    Inventors: Rachel Wezeman, Lin Rao, Kirk Cryer, Yan Wang
  • Patent number: 9272977
    Abstract: A process for purifying a filtered fermentation broth of succinic acid is described. The process involves separating succinic acid from the other organic acids and salts in a raw filtered broth using a non-functionalized chromatographic resin. The operation can be run either as an isocratic elution to yield free succinic acid, or as a gradient or binary elution to produce succinate salt.
    Type: Grant
    Filed: April 17, 2013
    Date of Patent: March 1, 2016
    Assignee: Archer Daniels Midland Company
    Inventors: John G. Soper, Mitchell Schultz, Thomas P. Binder