Liquid/liquid Or Gel Type (i.e., Jellylike) Chromatography Patents (Class 210/635)
  • Patent number: 10718743
    Abstract: A liquid chromatography (LC) column includes a wall having a length along a central axis from the inlet end to the outlet end, the wall enclosing a column interior and having a column radius relative to the central axis, the wall comprising a structured portion configured such that the column radius varies along the length; and a plurality of particles packed in the column interior, wherein at least some of the particles are in contact with the structured portion.
    Type: Grant
    Filed: August 2, 2018
    Date of Patent: July 21, 2020
    Assignee: Agilent Technologies, Inc.
    Inventors: Hongfeng Yin, Reid A. Brennen, Eric Lyster, Roger Slocum
  • Patent number: 10676503
    Abstract: A method for purifying a protein comprising an antibody, antibody fragment, or immunoglobulin single variable domain, from a solution containing at least one contaminant by superantigen chromatography comprising: a) adsorbing the protein to the superantigen immobilized on a solid support; b) removing the at least one contaminant by contacting the immobilized superantigen containing the adsorbed protein with a first wash buffer comprising an aliphatic carboxylate; and c) eluting the protein from the superantigen immobilized on the solid support.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: June 9, 2020
    Assignee: GLAXOSMITHKLINE INTELLECTUAL PROPERTY (NO.2) LIMITED
    Inventors: Kent E. Goklen, Eric J. Suda, Antonio Raul Ubiera
  • Patent number: 10571452
    Abstract: A system and a method are provided for calculating one or more indicative properties, e.g., one or more of the cetane number, octane number, pour point, cloud point and aniline point of oil fractions, from the density and high pressure liquid chromatography (HPLC) data of a sample of the crude oil.
    Type: Grant
    Filed: June 30, 2017
    Date of Patent: February 25, 2020
    Assignee: Saudi Arabian Oil Company
    Inventors: Omer Refa Koseoglu, Adnan Al-Hajji, Saroj Kumar Panda
  • Patent number: 10569190
    Abstract: Provided is a packed column capable of achieving sufficiently high distillation performance even with the height of its gas-liquid contactors reduced. The packed column is a packed column which includes a gas-liquid contactor 17, 18 inside a tubular body 16 and a liquid distributor 19 in the upper most portion and causes descending liquid and ascending gas to contact each other in the gas-liquid contactor. The operation pressure is in the range of 200 to 1500 kPaG. The relative volatility is in the range of 1.9 to 3.1. The gas-liquid contactor is vertically divided into at least two parts. A gas disperser 20 is provided at at least one position between a lower one of the gas-liquid contactors and an upper one of the gas-liquid contactors, the gas disperser uniformly dispersing the composition of the ascending gas rising from the lower gas-liquid contactor toward the upper gas-liquid contactor.
    Type: Grant
    Filed: March 3, 2017
    Date of Patent: February 25, 2020
    Assignee: TAIYO NIPPON SANSO CORPORATION
    Inventors: Takeyuki Ito, Nobuaki Egoshi
  • Patent number: 10487138
    Abstract: The present invention relates to the purification of immunoglobulins and the problem of providing a method for purifying an immunoglobulin in an efficient and cost-effective manner and with satisfactory purity and yield. In particular, the present invention addresses the aspect of the re-use of the rather cost-intensive chromatography materials, in particular the lifetime of the chromatography materials used in the capture step of the downstream process, and how this can be increased while reducing the technical complexity of the purification process.
    Type: Grant
    Filed: March 9, 2015
    Date of Patent: November 26, 2019
    Assignee: RICHTER GEDEON NYRT.
    Inventors: Ferenc Felföldi, Zsuzsa Benkö, Melinda Gáspár
  • Patent number: 10444216
    Abstract: A hand-held microfluidic testing device is provided that includes a housing having a cartridge receiving port and a cartridge for input to the cartridge receiving port. An optical detection system in the housing is capable of providing an illuminated electric field useful for Raman spectroscopy. The cartridge may have a sample well. The sample well is loaded with a mixture of water containing the analyte, Raman-scattering nanoparticles and a calibration solution. The calibration solution contains an analog of the analyte differing in its Raman response, for example an isotope of the analyte. Optionally, a chemical compound capable of increasing interaction between the analyte and the nanoparticles may be added.
    Type: Grant
    Filed: November 22, 2017
    Date of Patent: October 15, 2019
    Assignee: ONDAVIA, INC.
    Inventors: Mark Charles Peterman, Merwan Benhabib, Carlos Atico Ariza, Samuel Louis Kleinman
  • Patent number: 10408811
    Abstract: A crude oil composition estimation method estimates a composition of crude oil for use in simulation of a material balance in an absorption and liquefaction system that absorbs vapors emitted from the crude oil with the crude oil. The crude oil composition estimation method includes: comparing a calculated concentration of a treated gas with a measured concentration of the treated gas; estimating, when the calculated concentration agrees with the measured concentration, that the concentration included in the crude oil is a correct crude oil composition; and repeating, when the calculated concentration does not agree with the measured concentration, a process of correcting the concentration included in the crude oil and then comparing the calculated concentration with the measured concentration, until the calculated concentration agrees with the measured concentration, and when they agree, estimating that the corrected concentration in the crude oil is a correct crude oil composition.
    Type: Grant
    Filed: September 30, 2015
    Date of Patent: September 10, 2019
    Assignee: JFE Engineering Corporation
    Inventors: Yoshiki Shibuya, Kengo Murai
  • Patent number: 10399030
    Abstract: A Chromatography system that includes a microfluidic device configured to provide three-way switching or switching between three or more inputs or outputs. The microfluidic device fluidically coupled to one or more switching valves to provide for selective control of fluid flow in the chromatography system.
    Type: Grant
    Filed: September 4, 2017
    Date of Patent: September 3, 2019
    Assignee: PerkinElmer Health Sciences, Inc.
    Inventor: Andrew Tipler
  • Patent number: 10278405
    Abstract: The present invention concerns compositions and methods relating to the addition and use of par-baked and milled coffee beans in food products, beverages, and dietary supplements. Par-baked and milled coffee beans are a novel alternative to more costly chlorogenic acid extracts, and are edible and palatable as a food ingredient. The ingredient is prepared from the entire Coffea robusta or arabica green coffee bean and provides substantially increased amounts of dietary chlorogenic acid antioxidants compared to roasted beans. When the green coffee bean is appropriately par-baked at a temperature significantly lower than coffee roasting temperatures, the bean retains significant moisture, yet becomes embrittled, enabling milling to produce fine and beneficially hydrophilic particles, without any significant loss of bioactive chlorogenic acid and without significant pyrolysis occurring.
    Type: Grant
    Filed: March 8, 2018
    Date of Patent: May 7, 2019
    Assignee: Brandeis University
    Inventor: Daniel Perlman
  • Patent number: 10252186
    Abstract: In a solid phase extraction (SPE) apparatus, a cartridge housing tray houses a plurality of SPE cartridges. A liquid loading assembly applies liquid samples and SPE solutions into the plurality of cartridges. A liquid drawing assembly forces the liquid samples and the SPE solutions once they are applied to pass through the plurality of the SPE cartridges.
    Type: Grant
    Filed: August 9, 2016
    Date of Patent: April 9, 2019
    Assignee: ZIST ABZAR PAJOOHAN
    Inventor: Seyed Kamal Fatemi
  • Patent number: 10233487
    Abstract: A method of generating deformable gel beads that contain a single colloidal particle is described. Gel beads containing a particle are isolated from those that do not contain a particle, based on differences between the buoyant density of these gel bead populations. The gel beads containing a particle are subsequently co-encapsulated at a high efficiency into droplets that can comprise additional objects such as cells, other particles, or reagents. The gel coating on the rigid particle prevents clogging in narrow channels, such as those of a droplet generator.
    Type: Grant
    Filed: March 23, 2017
    Date of Patent: March 19, 2019
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Andrew Utada, Jeremy Agresti
  • Patent number: 10209377
    Abstract: A method of distinguishing effective pulses from test pulses in a scintillation detector that generates measurement light pulses includes providing a regularly-pulsed test light source that produces individual test light pulses having a time-dependent course of relative light intensity, which differs from a time-dependent course of relative light intensity of the measurement light pulses. The test light pulses are provided to a light detector for measurement of the test light pulses. The time-dependent courses of the relative light intensities of the test light pulses are analyzed. The measured pulses are separated into the test light pulses and the measurement light pulses according to the different time-dependent courses of the relative light intensities. The detector includes a scintillator, a light detector, a regularly-pulsed test light source that is adapted provide test light pulses to the light detector for measurement, and an electronic measuring circuit.
    Type: Grant
    Filed: November 8, 2004
    Date of Patent: February 19, 2019
    Assignee: FLIR Detection, Inc.
    Inventors: Jurgen Stein, Guntram Pausch
  • Patent number: 9951222
    Abstract: An object of the present invention is to provide a method for efficiently and highly selectively preparing a composition comprising perfluoropolyether having a hydroxyl group at one terminal at a higher ratio. The present invention provides a method for increasing a ratio of perfluoropolyether having a hydroxyl group at one terminal, relative to a total amount of the perfluoropolyether having a hydroxyl group at one terminal and perfluoropolyether having hydroxyl groups at both terminals in a composition comprising these perfluoropolyethers, wherein the method comprises a step of subjecting the composition to chromatography in which a moving phase is supercritical or subcritical state carbon dioxide of the specific temperature and the specific pressure, and a stationary phase is silica gel to thereby collect a fraction containing the perfluoropolyether having a hydroxyl group at one terminal at a higher ratio.
    Type: Grant
    Filed: April 9, 2015
    Date of Patent: April 24, 2018
    Assignee: SHIN-ETSU CHEMICAL CO., LTD.
    Inventors: Yuji Yamane, Takashi Matsuda, Noriyuki Koike
  • Patent number: 9950087
    Abstract: A biocidal treatment evaporates a liquid having biocidal properties. An absorption organ has absorbent strips suited to retain the liquid, each absorbent strip having a top first end and a bottom second end, The liquid is injected into the first end of each absorbent strip, the liquid flowing by gravity along the absorbent strip towards the second end, the liquid having biocidal properties being injected at a predetermined flow rate. The liquid is evaporated by producing a gas flow directed towards the absorption organ at a temperature below 50° C.
    Type: Grant
    Filed: October 9, 2015
    Date of Patent: April 24, 2018
    Assignee: XEDA INTERNATIONAL
    Inventor: Alberto Sardo
  • Patent number: 9936717
    Abstract: The present invention concerns compositions and methods relating to the addition and use of par-baked and milled coffee beans in food products, beverages, and dietary supplements. Par-baked and milled coffee beans are a novel alternative to more costly chlorogenic acid extracts, and are edible and palatable as a food ingredient. The ingredient is prepared from the entire Coffea robusta or arabica green coffee bean and provides substantially increased amounts of dietary chlorogenic acid antioxidants compared to roasted beans. When the green coffee bean is appropriately par-baked at a temperature significantly lower than coffee roasting temperatures, the bean retains significant moisture, yet becomes embrittled, enabling milling to produce fine and beneficially hydrophilic particles, without any significant loss of bioactive chlorogenic acid and without significant pyrolysis occurring.
    Type: Grant
    Filed: December 1, 2015
    Date of Patent: April 10, 2018
    Assignee: Brandeis University
    Inventor: Daniel Perlman
  • Patent number: 9938089
    Abstract: An automated positive pressure solid phase extraction apparatus and method comprising two tiered lifts devices each individually controllable to individually vertically translate within an elevator framework between a base on which the elevator framework is mounted and a manifold plate supported by the framework in a substantially horizontal plane parallel with and vertically above the two tiered lifts devices and a rectilinearly translating shuttle assembly comprising a shuttle supporting labware for rectilinear travel into and out of the elevator framework to handoff the labware to one of the two tiered elevator lifts or both.
    Type: Grant
    Filed: January 16, 2015
    Date of Patent: April 10, 2018
    Assignee: Hamilton Company
    Inventors: Garrett Voss, Harrison Elings
  • Patent number: 9925478
    Abstract: Methods for purifying nanocrystals via gel permeation chromatography are provided. The method can include: loading a sample solution into a chromatography column, wherein the sample solution comprises nanocrystals coated with a layer of organic ligands; using an eluent, passing the nanocrystals coated with the layer of organic ligands through a stationary phase of the chromatography column, wherein the eluent is an organic solvent in which the nanocrystals coated with the layer of organic ligands remain miscible to form a mobile phase; and collecting the mobile phase passed through the stationary phase of the chromatography column. The surface ligands of the purified nanocrystals can then be, in one embodiment, replaced with a second type of ligand.
    Type: Grant
    Filed: June 27, 2014
    Date of Patent: March 27, 2018
    Assignee: University of South Carolina
    Inventors: Andrew B. Greytak, Yi Shen
  • Patent number: 9911520
    Abstract: Disclosed herein are monolayers comprising alkylamine-gold nanoparticles that have tunable electrical and optical properties. Also disclosed is a method for forming the monolayers that comprises self-assembly of the nanoparticles.
    Type: Grant
    Filed: October 19, 2016
    Date of Patent: March 6, 2018
    Assignee: FLORIDA STATE UNIVERSITY RESEARCH FOUNDATION, INC.
    Inventors: Daniel Hallinan, Jr., Guang Yang
  • Patent number: 9890205
    Abstract: A method of purifying a sample containing a desired protein includes the steps of (i) providing a packed chromatographic column having positively charged porous particles, (ii) equilibrating the column to the conditions to which the desired protein in the sample is to elute, (iii) contacting the sample with the packed chromatographic column such that the sample volume applied to the packed chromatographic column is less than or equal to the interparticle space of the positively charged porous particles within the packed chromatographic column, (iv) eluting the desired protein from the packed chromatographic column, where the desired protein is in a purer state and in the conditions to which the packed chromatographic column was equilibrated; where the desired protein is an antibody, an antibody fragment, an antibody derivative, or an antibody fusion protein.
    Type: Grant
    Filed: November 26, 2014
    Date of Patent: February 13, 2018
    Assignee: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
    Inventor: Peter Gagnon
  • Patent number: 9879308
    Abstract: Systems and methods for culturing and monitoring, ex vivo, pharmacologic and metabolic response in a biological sample, including receiving at a fluidic apparatus the biological sample retrieved from the patient, retaining the biological sample within a channel of the fluidic apparatus, providing for the culture of the biological sample within the channel of the fluidic apparatus, flowing a fluid past the biological sample, retrieving and analyzing the fluid to determine a pharmacologic and/or metabolic response of the sample.
    Type: Grant
    Filed: December 30, 2014
    Date of Patent: January 30, 2018
    Assignee: The Charles Stark Draper Laboratory, Inc.
    Inventors: Angela Holton, Alla Gimbel, David Landis, Abigail Spencer
  • Patent number: 9863924
    Abstract: A hand-held microfluidic testing device is provided that includes a housing having a cartridge receiving port and a cartridge for input to the cartridge receiving port. An optical detection system in the housing is capable of providing an illuminated electric field useful for Raman spectroscopy. The cartridge may have a sample well. The sample well is loaded with a mixture of water containing the analyte, Raman-scattering nanoparticles and a calibration solution. The calibration solution contains an analog of the analyte differing in its Raman response, for example an isotope of the analyte. Optionally, a chemical compound capable of increasing interaction between the analyte and the nanoparticles may be added.
    Type: Grant
    Filed: September 16, 2016
    Date of Patent: January 9, 2018
    Assignee: OndaVia, Inc.
    Inventors: Mark Charles Peterman, Merwan Benhabib, Carlos Atico Ariza, Samuel Louis Kleinman
  • Patent number: 9827552
    Abstract: A solid phase for use in separation has been modified using an aqueous phase adsorption of a headgroup-modified lipid to generate analyte specific surfaces for use as a stationary phase in separations such as high performance liquid chromatography (HPLC) or solid phase extraction (SPE). The aliphatic moiety of the lipid adsorbs strongly to a hydrophobic solid surface, with the hydrophilic and active headgroups orienting themselves toward the more polar mobile phase, thus allowing for interactions with the desired solutes. The surface modification approach is generally applicable to a diversity of selective immobilization applications such as protein immobilization clinical diagnostics and preparative scale HPLC as demonstrated on capillary-channeled fibers, though the general methodology could be implemented on any hydrophobic solid support material.
    Type: Grant
    Filed: July 17, 2014
    Date of Patent: November 28, 2017
    Assignee: Clemson University
    Inventors: R. Kenneth Marcus, Kenneth A. Christensen
  • Patent number: 9733239
    Abstract: Reconfigurable microfluidic systems are based on networks of microfluidic cavities connected by hydrophobic microfluidic channels. Each cavity is classified as either a reservoir or a node, and includes a pressure port via which gas pressure may be applied. Sequences of gas pressures, applied to reservoirs and nodes according to a fluid transfer rule, enable fluid to be moved from any reservoir to any other reservoir in a system. Systems may be configured with multiple switched interaction regions connected in series for scalable, multiplexed immunoassays. Multiple, switched interaction regions may also be implemented with microvalves.
    Type: Grant
    Filed: July 24, 2015
    Date of Patent: August 15, 2017
    Assignee: HJ Science & Technology, Inc.
    Inventors: Hong Jiao, Erik C Jensen, Homayun Mehrabani, Liran Yosef Haller
  • Patent number: 9663553
    Abstract: The invention is an integrated process for the isolation and purification of plasma derived clotting Factor VIII, clotting Factor IX, albumin and immunoglobulin without the use of ethanol precipitation. The integrated process comprises of sequential chromatography steps of gel filtration, anion exchange and cation exchange chromatographies, followed by viral inactivation and removal steps. The present invention has the advantage of being a mild process that does not denature or aggregate the proteins and provides high yields of several therapeutic grade plasma proteins from a given volume of plasma.
    Type: Grant
    Filed: November 3, 2014
    Date of Patent: May 30, 2017
    Assignee: HEMARUS THERAPEUTICS LIMITED
    Inventors: Neelima Vadde, Mitali Samaddar, Ashok Nuvula, Zinia Chakraborty, Swapna Sagar Duggineni, Uma Devi Komath
  • Patent number: 9631007
    Abstract: A method for the purification of immunoglobulins by ion exchange chromatography is described. The chromatographic method uses a weak ion exchange resin and a single step elution process for the purification of an immunoglobulin. Additionally a method for the determination of the salt concentration for the single step elution of an immunoglobulin from an ion exchange resin is described.
    Type: Grant
    Filed: May 6, 2014
    Date of Patent: April 25, 2017
    Assignee: HOFFMANN-LA-ROCHE INC.
    Inventors: Roberto Falkenstein, Burkard Kolb, Maria Sebald
  • Patent number: 9511575
    Abstract: In one or more embodiments, a porous composite particulate material includes a plurality of composite particles including an acid-base-resistant core particle at least partially surrounded by one or more layers of acid-base-resistant shell particles. The shell particles are adhered to the core particle by a polymeric material. The shell particles and/or core particles may be made from an acid-base-resistant material that is stable in harsh chemical conditions. During application of the polymeric material/shell particle bilayer, the core particles are sonicated to homogenize the particle size distribution and minimize agglomeration of particles. Multiple bilayers of polymer/shell particles may be applied. In one embodiment, the core particle comprises generally spherical glassy carbon, while the shell particles may comprise nano-sized diamond particles. Other acid-base-resistant materials may be employed.
    Type: Grant
    Filed: January 10, 2014
    Date of Patent: December 6, 2016
    Assignee: BRIGHAM YOUNG UNIVERSITY
    Inventors: Matthew R. Linford, Chuan-Hsi Hung
  • Patent number: 9458715
    Abstract: Methods and devices for determining a plus fraction of a plus fraction of a gas chromatogram are provided. A gas chromatogram may obtained, such as from a downhole gas chromatograph module of a fluid analysis tool. The plus fraction of the gas chromatogram may be determined using one or more of a ratiometric determination, fitting an exponential decay function, and fitting a probability density gamma function.
    Type: Grant
    Filed: December 16, 2014
    Date of Patent: October 4, 2016
    Assignee: SCHLUMBERGER TECHNOLOGY CORPORATION
    Inventors: Ronald E. G. van Hal, Jeffrey Crank, Youxiang Zuo, Adriaan Gisolf
  • Patent number: 9417220
    Abstract: Petroleum or other hydrocarbon samples can be analyzed in parallel by 1) GC-field ionization Time of Flight Mass Spectrometer (GC-FI-TOF MS) and 2) two dimensional gas chromatography (2D-GC) equipped with a flame ionization detector (FID). The combined techniques allow for improved quantitative characterization of the compounds within a hydrocarbon sample. The techniques can be combined by correlating the 2D-GC FID data with the GC-FI-TOF MS data based on correlation of compound classes, correlation of retention windows within a compound class, correlation of individual compounds, such as paraffins, or a combination thereof.
    Type: Grant
    Filed: October 23, 2013
    Date of Patent: August 16, 2016
    Assignee: EXXONMOBIL RESEARCH AND ENGINEERING COMPANY
    Inventors: Frank C. Wang, Kuangnan Qian, Kathleen E. Edwards
  • Patent number: 9345989
    Abstract: A drive mechanism for back pressure regulator used in liquid chromatography, supercritical fluid chromatography, or supercritical fluid extraction allows very fine automated control over a very wide range of pressures by combining a linear actuator compressing a spring, pushing a pin. The nozzle assembly of the regulator comprises a flow through chamber containing a diaphragm and a seat, in which the pin pushes the diaphragm against the seat, together with an upstream pressure sensor and electronic feedback control to the motor of the actuator. The BPR of the embodiments exhibits high pressure stability and extremely low pressure noise, even at moderate to high pressures. The exemplary BPR can be use at either constant pressure or to generate pressure programs where the pressure is varied versus time. Further, the nozzle assembly has a field-replaceable head, requiring no mechanical adjustment on replacement.
    Type: Grant
    Filed: April 12, 2013
    Date of Patent: May 24, 2016
    Assignee: Agilent Technologies, Inc.
    Inventors: Terry A. Berger, Samuel Colgate, Michael Casale
  • Patent number: 9340562
    Abstract: A chromatographic material comprising a zwitterionic ligand covalently bound to a substrate, the ligand preferably has a formula II: wherein R1, R2, R3 are independently selected from an oxygen atom that is configured to connect to a substrate atom in the substrate, an oxygen atom that is configured to connect to a silicon atom of an adjacent ligand, a hydroxyl group, a halogen atom, an alkoxy group, a dialkylamino group, an acyl group, an alkyl group, or an aryl group; L1, L2 and L3 are independently hydrophobic moieties; each containing 2 to 30 carbon atoms, wherein there are at least 10 carbon atoms in the combined chain lengths of L1, L2 and L3; X is an O atom, S atom, amide group or sulfonamide group; n is 0 or 1; R4, R5 are independently selected from a hydrogen atom or a hydrocarbon moiety containing 1 to 20 carbon atoms; and Rf is a negatively charged moiety comprising a sulfonic, carboxylic, or phosphonic functional group.
    Type: Grant
    Filed: October 29, 2014
    Date of Patent: May 17, 2016
    Assignees: Thermo Electron Manufacturing Limited, Dionex Corporation
    Inventors: Xiaodong Liu, Richard T. Williams, Xiao Cui
  • Patent number: 9303098
    Abstract: The invention relates to a process for the preparation of a separating material having improved binding capacity, and to materials prepared and to the use thereof for the separation of charged or uncharged biopolymers from liquids.
    Type: Grant
    Filed: May 5, 2009
    Date of Patent: April 5, 2016
    Assignee: MERCK PATENT GMBH
    Inventor: Matthias Joehnck
  • Patent number: 9295928
    Abstract: A porous substrate capable of adsorptive filtration of a fluid having a porous self-supporting substrate and one or more porous, adsorptive polymeric coatings comprising from about 1 to about 80% of the void volume of the pores of the substrate. The resultant substrate has good convective and diffusive flow and capacity. The substrate may be cross-linked, have one or more capture chemistries attached to it and is useful as a chromatography media for the selective filtration of desired species including biomolecules such as proteins and DNA fragments.
    Type: Grant
    Filed: November 2, 2010
    Date of Patent: March 29, 2016
    Assignee: EMD Millipore Corporation
    Inventors: Anthony J. DiLeo, Justin McCue, Wilson Moya, Igor Quinones-Garcia, Neil P. Soice, Volkmar Thom, Sarah Yuan
  • Patent number: 9291630
    Abstract: Disclosed is a method for the separation, identification and quantification of multiple proteins in pancreatin active pharmaceutical ingredient samples.
    Type: Grant
    Filed: August 29, 2012
    Date of Patent: March 22, 2016
    Assignee: Scientific Protein Laboratories, LLC
    Inventors: Rachel Wezeman, Lin Rao, Kirk Cryer, Yan Wang
  • Patent number: 9272977
    Abstract: A process for purifying a filtered fermentation broth of succinic acid is described. The process involves separating succinic acid from the other organic acids and salts in a raw filtered broth using a non-functionalized chromatographic resin. The operation can be run either as an isocratic elution to yield free succinic acid, or as a gradient or binary elution to produce succinate salt.
    Type: Grant
    Filed: April 17, 2013
    Date of Patent: March 1, 2016
    Assignee: Archer Daniels Midland Company
    Inventors: John G. Soper, Mitchell Schultz, Thomas P. Binder
  • Patent number: 9274092
    Abstract: A method and device for packing a chromatography column formed of one or more vibration devices attached to top and/or bottom flanges of the column. Media is added in one or more steps to the column, allowed to settle under the effects of gravity and then subjected to one or more treatments of vibration from the vibration devices until a suitably packed column is obtained. Liquid used to suspend the media while being placed into the column may be at least partially removed before or during the vibration step(s). The remaining liquid is then removed or replaced after the packing has been obtained.
    Type: Grant
    Filed: August 6, 2015
    Date of Patent: March 1, 2016
    Assignee: EMD Millipore Corporation
    Inventors: Venkatesh Natarajan, Anthony Frederick Mann, Daniel Schubnel
  • Patent number: 9266041
    Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.
    Type: Grant
    Filed: June 13, 2011
    Date of Patent: February 23, 2016
    Assignee: GE Healthcare Bio-Sciences AB
    Inventors: Bo-Lennart Johansson, Jean-Luc Maloisel, Hans J. Johansson, Asa Ek, Maria Holm, Jafar Saidzadeh
  • Patent number: 9259687
    Abstract: The invention concerns a bag comprising a first conduit (13C) which extends longitudinally between a flow pump connector (11C) emerging on a first side (68) and a tangential filter connector (11M) emerging on a second side (69); a second conduit (13B) which extends longitudinally from a first side of said conduit (13C) between a supply container connector (11B) emerging on said first side (68), and another tangential flow connector (11N) emerging on said second side (69); a third conduit (13H) which extends from a second side of said conduit (13C), starting at a collecting container connector (11J), until it enters said first conduit (13C); and a fourth conduit (13A) which extends from the first side of said conduit (13C), starting at a transfer pump connector (11A), until it enters said second conduit (13B).
    Type: Grant
    Filed: May 8, 2013
    Date of Patent: February 16, 2016
    Assignee: EMD Millipore Corporation
    Inventors: Jean-Louis Weissenbach, Sebastien Cirou
  • Patent number: 9227173
    Abstract: A method for dynamic filtration of a pharmaceutical product is provided. The method includes using an unconditioned resin configured to selectively retain one or more components from a mixture having the pharmaceutical product and where the unconditioned resin is configured to be activated by a medium of the mixture. The method further includes the use of at least one positioning material disposed adjacent to the unconditioned resin, where the positioning material is configured to provide mechanical support to the resin to at least partially retain the resin in position.
    Type: Grant
    Filed: November 9, 2011
    Date of Patent: January 5, 2016
    Assignee: General Electric Company
    Inventors: Andrew Michael Leach, Peter Miller, Eric John Telfeyan, David Brandon Whitt
  • Patent number: 9220803
    Abstract: A medical composition is disclosed, which is injectable and which comprises a mixture of native components, which are obtainable by culturing one or more cell samples from a human or animal during normal conditions, said native components being included in the group consisting of growth factors, extracellular matrix proteins, and other substances produced by said cell samples during normal conditions, and a pharmaceutically acceptable carrier, as well as a method for producing the native components, a method for producing the medical composition, a method for treating a subject in need of tissue repair by injection of the medical composition, and use of said mixture of native components for the production of said medical composition for tissue repair via injection.
    Type: Grant
    Filed: October 2, 2007
    Date of Patent: December 29, 2015
    Inventor: Lars Lidgren
  • Patent number: 9216366
    Abstract: The present invention aims to provide a liquid chromatography component including a column and a prefilter, which is hard to cause an increase of supplied liquid pressure even when the measurement of a sample is repeated. The present invention is a liquid chromatography component, which includes: a column with filler particles filled therein; and a prefilter, the filler particles having an average particle size in the range of 2 to 20 ?m, the prefilter having a filtering particle size in the range of ? to ? of the average particle size of the filler particles.
    Type: Grant
    Filed: October 10, 2013
    Date of Patent: December 22, 2015
    Assignee: SEKISUI CHEMICAL CO., LTD.
    Inventor: Takuya Yotani
  • Patent number: 9212243
    Abstract: Soil adsorption polymers and method for making same are provided.
    Type: Grant
    Filed: August 30, 2012
    Date of Patent: December 15, 2015
    Assignee: The Procter & Gamble Company
    Inventors: Steven Daryl Smith, Robert Joseph McChain, Robin Lynn McKiernan, Charles William Neal
  • Patent number: 9205351
    Abstract: A collection system for collecting samples from a flowstream (5) exiting a supercritical fluid chromatography system is provided. The collection system comprises: (i) a first back pressure regulator (10) on the flowstream as it exits the chromatography system, (ii) a gas-liquid separator (30) having a tapered and angled dripper (65), which introduces the flow into the separator at an angle tangential to the separator wall; and (iii) one or more fraction collectors (15), wherein the fraction collector is at a reduced pressure collection point, between 100 bar and atmospheric pressure. A collection system for HPLC is also provided, as well as a process for collection of samples from a flowstream.
    Type: Grant
    Filed: November 20, 2013
    Date of Patent: December 8, 2015
    Assignee: Waters Technologies Corporation
    Inventors: Harbaksh Sidhu, Ziqiang Wang, Steven L. Zulli
  • Patent number: 9205955
    Abstract: The invention concerns a device comprising a base; a circuit comprising a bag comprising two flexible films and conveying network connectors, and a press (9) comprising a first shell (16) disposed upright on a front face (5) of said base (2) and a second shell (17) mounted on said first shell (16), said first shell (16) and second shell (17) clamping said bag to form conduits; said bag comprising first through apertures on one side and said first shell (16) comprising hooking studs (106) on an upper part, which studs (106) pass through said first apertures.
    Type: Grant
    Filed: June 6, 2011
    Date of Patent: December 8, 2015
    Assignee: EMD Millipore Corporation
    Inventors: Sebastien Cirou, Rene Reinbigler, Virginie Buisson, Jean-Louis Weissenbach
  • Patent number: 9181323
    Abstract: The invention concerns a concentrate of human Factor XI having high specific activity prepared using a method comprising a filtration-adsorption step and a chromatography step on cation exchange resin. The concentrate obtained is fully adapted for therapeutic use as substitution therapy in cases of Factor XI deficiency.
    Type: Grant
    Filed: April 19, 2012
    Date of Patent: November 10, 2015
    Assignee: LABORATOIRE FRANCAIS DU FRACTIONNEMENT ET DES BIOTECHNOLOGIES
    Inventor: Jean-François Martin
  • Patent number: 9174772
    Abstract: The invention concerns a device comprising a base; a circuit comprising a bag comprising two flexible films and conveying network connectors, and a press (9) comprising a first shell (16) disposed upright on a front face (5) of said base (2) and a second shell (17) mounted on said first shell (16), said first shell (16) and second shell (17) clamping said bag to form conduits; said bag comprising first through apertures on one side and said first shell (16) comprising hooking studs (106) on an upper part, which studs (106) pass through said first apertures.
    Type: Grant
    Filed: June 6, 2011
    Date of Patent: November 3, 2015
    Assignee: EMD Millipore Corporation
    Inventors: Sebastien Cirou, Rene Reinbigler, Virginie Buisson, Jean-Louis Weissenbach
  • Patent number: 9174171
    Abstract: The invention concerns a bag comprising a first conduit (13C) which extends longitudinally between a flow pump connector (11C) emerging on a first side (68) and a tangential filter connector (11M) emerging on a second side (69); a second conduit (13B) which extends longitudinally from a first side of said conduit (13C) between a supply container connector (11B) emerging on said first side (68), and another tangential flow connector (11N) emerging on said second side (69); a third conduit (13H) which extends from a second side of said conduit (13C), starting at a collecting container connector (11J), until it enters said first conduit (13C); and a fourth conduit (13A) which extends from the first side of said conduit (13C), starting at a transfer pump connector (11A), until it enters said second conduit (13B).
    Type: Grant
    Filed: June 16, 2011
    Date of Patent: November 3, 2015
    Assignee: EMD Millipore Corporation
    Inventors: Jean-Louis Weissenbach, Sebastien Cirou
  • Patent number: 9163050
    Abstract: Disclosed is a process for the production of d-mannose from fermented palm oil kernel meal using a continuous SMB separation process. The process is useful for providing a simplified processing route to providing high purity d-mannose. The SMB process and the SMB cycle was operated to provide a high purity mannose stream comprising d-mannose, salts, and color agents, a primary raffinate comprising glucose, other sugars and salts, and a secondary raffinate consisting essentially of the mobile phase desorbent. In the SMB cycle, the secondary raffinate was recycled to the SMB process as the mobile phase desorbent without further desalination. The highly pure mannose stream was further treated to remove color agents and salts prior to subsequent steps of precipitation or crystallization and drying. D-mannose is useful as a food additive, as a sweetener, as a texturizer, as a stabilizer, or as a humectant.
    Type: Grant
    Filed: August 6, 2012
    Date of Patent: October 20, 2015
    Assignee: OROCHEM TECHNOLOGIES, INC.
    Inventors: Anil R Oroskar, Naveen S Sudharsan, Priyanka Anil Oroskar, Omkar M Kulkarni
  • Patent number: 9155979
    Abstract: A method for performing maintenance on a chromatography column (1; 41) comprising the steps of: a) when maintenance is required, detaching a detachable joint (7; 63) between a bottom plate (5; 53) of the chromatography column and a stand (3; 43) on which the chromatography column is provided; b) attaching an adaptor (15; 59) provided in the chromatography system to any part of the chromatography column (1; 41) making it possible to lift the bottom plate (5; 53) from the stand (3; 43) with the adaptor (15; 59); and c) raising the adaptor (15; 59) and thereby also the bottom plate (5; 53) such that maintenance can be performed under the bottom plate (5; 53).
    Type: Grant
    Filed: January 20, 2014
    Date of Patent: October 13, 2015
    Assignee: GE Healthcare Bio-Sciences AB
    Inventors: Manoj Kumar Ramakrishna, Daniel Salomonsson, Per Uselius
  • Patent number: 9155980
    Abstract: The present invention relates to a separation medium, comprising an inner core of a porous material provided with charged ligands, and an outer lid comprising a porous material provided with charged ligands, wherein the charge of the ligands in the inner core is opposite that of the charge of the ligands in the lid. The present invention also relates to a method for biomolecule separation comprising applying a sample to the above separation medium, wherein large molecules are prevented from entering the medium by charge repulsion from the medium and small molecules are captured in the inner core.
    Type: Grant
    Filed: February 20, 2013
    Date of Patent: October 13, 2015
    Assignee: GE Healthcare Bio-Sciences AB
    Inventors: Gunnar Glad, Bo-Lennart Johansson, Jean-Luc Maloisel, Nils Norrman
  • Patent number: 9150816
    Abstract: A method for purification of a first polyunsaturated fatty acid from an initial mixture is provided. The initial mixture comprises at least one second fatty acid in addition to the first polyunsaturated fatty acid. The method may comprise separating the first polyunsaturated fatty acid and the second fatty acid in a liquid phase in at least one step of a chromatographic separation process, so as to recover, on the one hand, a stream enriched in the first polyunsaturated fatty acid, and on the other hand, a stream enriched in the second fatty acid. The method may further comprise a step of processing the stream enriched in the first polyunsaturated fatty acid to decrease the peroxide value and/or anisidine value of this stream. The disclosure also provides systems or devices suitably adapted for the implementation of such methods.
    Type: Grant
    Filed: October 22, 2014
    Date of Patent: October 6, 2015
    Assignee: Novasep Process SAS
    Inventors: Roger-Marc Nicoud, Jean Bléhaut