Abstract: A process for purifying a filtered fermentation broth of succinic acid is described. The process involves separating succinic acid from the other organic acids and salts in a raw filtered broth using a non-functionalized chromatographic resin. The operation can be run either as an isocratic elution to yield free succinic acid, or as a gradient or binary elution to produce succinate salt.

Abstract: A method and device for packing a chromatography column formed of one or more vibration devices attached to top and/or bottom flanges of the column. Media is added in one or more steps to the column, allowed to settle under the effects of gravity and then subjected to one or more treatments of vibration from the vibration devices until a suitably packed column is obtained. Liquid used to suspend the media while being placed into the column may be at least partially removed before or during the vibration step(s). The remaining liquid is then removed or replaced after the packing has been obtained.

Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.

Abstract: The invention concerns a bag comprising a first conduit (13C) which extends longitudinally between a flow pump connector (11C) emerging on a first side (68) and a tangential filter connector (11M) emerging on a second side (69); a second conduit (13B) which extends longitudinally from a first side of said conduit (13C) between a supply container connector (11B) emerging on said first side (68), and another tangential flow connector (11N) emerging on said second side (69); a third conduit (13H) which extends from a second side of said conduit (13C), starting at a collecting container connector (11J), until it enters said first conduit (13C); and a fourth conduit (13A) which extends from the first side of said conduit (13C), starting at a transfer pump connector (11A), until it enters said second conduit (13B).

Abstract: A method for dynamic filtration of a pharmaceutical product is provided. The method includes using an unconditioned resin configured to selectively retain one or more components from a mixture having the pharmaceutical product and where the unconditioned resin is configured to be activated by a medium of the mixture. The method further includes the use of at least one positioning material disposed adjacent to the unconditioned resin, where the positioning material is configured to provide mechanical support to the resin to at least partially retain the resin in position.

Abstract: A medical composition is disclosed, which is injectable and which comprises a mixture of native components, which are obtainable by culturing one or more cell samples from a human or animal during normal conditions, said native components being included in the group consisting of growth factors, extracellular matrix proteins, and other substances produced by said cell samples during normal conditions, and a pharmaceutically acceptable carrier, as well as a method for producing the native components, a method for producing the medical composition, a method for treating a subject in need of tissue repair by injection of the medical composition, and use of said mixture of native components for the production of said medical composition for tissue repair via injection.

Abstract: The present invention aims to provide a liquid chromatography component including a column and a prefilter, which is hard to cause an increase of supplied liquid pressure even when the measurement of a sample is repeated. The present invention is a liquid chromatography component, which includes: a column with filler particles filled therein; and a prefilter, the filler particles having an average particle size in the range of 2 to 20 ?m, the prefilter having a filtering particle size in the range of ? to ? of the average particle size of the filler particles.

Abstract: Soil adsorption polymers and method for making same are provided.

Abstract: A collection system for collecting samples from a flowstream (5) exiting a supercritical fluid chromatography system is provided. The collection system comprises: (i) a first back pressure regulator (10) on the flowstream as it exits the chromatography system, (ii) a gas-liquid separator (30) having a tapered and angled dripper (65), which introduces the flow into the separator at an angle tangential to the separator wall; and (iii) one or more fraction collectors (15), wherein the fraction collector is at a reduced pressure collection point, between 100 bar and atmospheric pressure. A collection system for HPLC is also provided, as well as a process for collection of samples from a flowstream.

Abstract: The invention concerns a device comprising a base; a circuit comprising a bag comprising two flexible films and conveying network connectors, and a press (9) comprising a first shell (16) disposed upright on a front face (5) of said base (2) and a second shell (17) mounted on said first shell (16), said first shell (16) and second shell (17) clamping said bag to form conduits; said bag comprising first through apertures on one side and said first shell (16) comprising hooking studs (106) on an upper part, which studs (106) pass through said first apertures.

Abstract: The invention concerns a concentrate of human Factor XI having high specific activity prepared using a method comprising a filtration-adsorption step and a chromatography step on cation exchange resin. The concentrate obtained is fully adapted for therapeutic use as substitution therapy in cases of Factor XI deficiency.

Abstract: The invention concerns a bag comprising a first conduit (13C) which extends longitudinally between a flow pump connector (11C) emerging on a first side (68) and a tangential filter connector (11M) emerging on a second side (69); a second conduit (13B) which extends longitudinally from a first side of said conduit (13C) between a supply container connector (11B) emerging on said first side (68), and another tangential flow connector (11N) emerging on said second side (69); a third conduit (13H) which extends from a second side of said conduit (13C), starting at a collecting container connector (11J), until it enters said first conduit (13C); and a fourth conduit (13A) which extends from the first side of said conduit (13C), starting at a transfer pump connector (11A), until it enters said second conduit (13B).

Abstract: The invention concerns a device comprising a base; a circuit comprising a bag comprising two flexible films and conveying network connectors, and a press (9) comprising a first shell (16) disposed upright on a front face (5) of said base (2) and a second shell (17) mounted on said first shell (16), said first shell (16) and second shell (17) clamping said bag to form conduits; said bag comprising first through apertures on one side and said first shell (16) comprising hooking studs (106) on an upper part, which studs (106) pass through said first apertures.

Abstract: Disclosed is a process for the production of d-mannose from fermented palm oil kernel meal using a continuous SMB separation process. The process is useful for providing a simplified processing route to providing high purity d-mannose. The SMB process and the SMB cycle was operated to provide a high purity mannose stream comprising d-mannose, salts, and color agents, a primary raffinate comprising glucose, other sugars and salts, and a secondary raffinate consisting essentially of the mobile phase desorbent. In the SMB cycle, the secondary raffinate was recycled to the SMB process as the mobile phase desorbent without further desalination. The highly pure mannose stream was further treated to remove color agents and salts prior to subsequent steps of precipitation or crystallization and drying. D-mannose is useful as a food additive, as a sweetener, as a texturizer, as a stabilizer, or as a humectant.

Abstract: A method for performing maintenance on a chromatography column (1; 41) comprising the steps of: a) when maintenance is required, detaching a detachable joint (7; 63) between a bottom plate (5; 53) of the chromatography column and a stand (3; 43) on which the chromatography column is provided; b) attaching an adaptor (15; 59) provided in the chromatography system to any part of the chromatography column (1; 41) making it possible to lift the bottom plate (5; 53) from the stand (3; 43) with the adaptor (15; 59); and c) raising the adaptor (15; 59) and thereby also the bottom plate (5; 53) such that maintenance can be performed under the bottom plate (5; 53).

Abstract: The present invention relates to a separation medium, comprising an inner core of a porous material provided with charged ligands, and an outer lid comprising a porous material provided with charged ligands, wherein the charge of the ligands in the inner core is opposite that of the charge of the ligands in the lid. The present invention also relates to a method for biomolecule separation comprising applying a sample to the above separation medium, wherein large molecules are prevented from entering the medium by charge repulsion from the medium and small molecules are captured in the inner core.

Abstract: A method for purification of a first polyunsaturated fatty acid from an initial mixture is provided. The initial mixture comprises at least one second fatty acid in addition to the first polyunsaturated fatty acid. The method may comprise separating the first polyunsaturated fatty acid and the second fatty acid in a liquid phase in at least one step of a chromatographic separation process, so as to recover, on the one hand, a stream enriched in the first polyunsaturated fatty acid, and on the other hand, a stream enriched in the second fatty acid. The method may further comprise a step of processing the stream enriched in the first polyunsaturated fatty acid to decrease the peroxide value and/or anisidine value of this stream. The disclosure also provides systems or devices suitably adapted for the implementation of such methods.

Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.

Abstract: The present invention relates to recombinant furin (rFurin) and methods for producing rFurin. More specifically, the invention relates to substantially animal protein-free rFurin and methods for producing substantially animal protein-free rFurin.

Abstract: The present invention relates to a process of enriching one target compound from a liquid, which process comprises at least one step of isolation performed by differentially partitioning between two aqueous phases. In the present invention the phases are formed by adding a thermally responsive, self-associating (i.e. clouding) hydrophilic polymer, and if needed some additional salts, to an aqueous biotechnical solution (such as a fermentation sample or bioseparation process stream) under thermal and other conditions where the solution separates into a one polymer, two-phase system with one phase enriched in the polymer. The target compound is to be found in the phase not enriched in the polymer, while a significant though varying percentage of contaminants may differentially partition to the phase interface or the polymer enriched phase.

Abstract: A process for the separation of a xylose-containing, lignosulphonate-containing Ca-sulfite spent liquor is provided. The process comprises a first step of: (A) chromatographic separation of the Ca- or Mg-sulfite spent liquor using a single pass through a batch-type long resin bed chromatography column having a strong acid cation exchange resin as a separation medium. The ratio of the bed length:mean resin bead diameter of the column is 10,000-40,000. This step yields •a first xylose-rich fraction having a xylose content 50-70 wt. % based on dry solids and • a lignosulphonate-rich fraction. The process further comprises a step of: (B) crystallizing xylose from the first xylose rich fraction by using a crystallization method comprising (i) boiling crystallization; (ii) cooling crystallization or (iii) a combination of (i) and (ii) to obtain a first batch of crystalline xylose and a first xylose run-off.

Abstract: Described herein are methods for isolating and purifying antibodies from a sample matrix. One aspect of the present disclosure is directed to viral reduction/inactivation of samples generated in the various steps of antibody purification. In a particular aspect, methods herein employ an acidification step followed by one or more chromatography steps. The chromatography steps can include one or more of the following chromatographic procedures: ion exchange chromatography, affinity chromatography, and hydrophobic interaction chromatography.

Abstract: The present invention relates to cells (1) for a centrifugal partition chromatography column, consisting of stacked discs and comprising a network of three-dimensional cells interconnected in series and communicating with liquid phase circulation channels, said cells being distributed over the periphery of at least one disc driven into rotation about a main axis (8); the cells have a geometric shape of revolution about a substantially radial axis with respect to said disc and they are connected by channels (2, 4) of substantially circular, elliptical or parallelepipedic section, the main two dimensions of which are smaller than the largest cross-section of the cell. The invention also relates to methods of manufacturing these cells.

Abstract: A voltage control circuitry for a detection cell is described, where the detection cell is adapted for determining an electrical property of a sample in a detection cell volume of the detection cell. The voltage control circuitry comprises a power supply adapted for providing a voltage to the detection cell, and a power evaluation unit adapted for determining an actual power dissipation in the detection cell volume. The voltage control circuitry further comprises a control unit adapted for comparing the actual power dissipation with a desired power dissipation, and for regulating the power supply's voltage in a way that the actual power dissipation is driven towards the desired power dissipation.

Abstract: An electrolytic CO2-removal device for anion analysis of a liquid sample. The device includes a basic chamber and CO2-permeable tubing in the basic chamber. Anion exchange membranes are disposed on opposite sides of the basic chamber, and electrodes are disposed outside the membranes. The device can be integral with a suppressor in an ion chromatography system and/or an aqueous stream purifier. Also, methods performed by the device.

Abstract: The present invention further is directed to the process for making a macroporous polymeric media with covalently bonded quaternary ammonium polymer chains. The media can be applied for chromatographic purification, resulting in porous substrates having improved protein binding capacity and resin selectivity, as well as methods relating to making and using the same.

Abstract: Disclosed herein are compositions and methods for the isolation and purification of proteins from a sample. In particular, the present invention relates to compositions and methods for isolating and purifying proteins incorporating a displacement chromatographic step. The present invention is also directed toward pharmaceutical compositions comprising one or more antibodies purified by a method described herein.

Abstract: The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.

Abstract: The present invention relates to a separation matrix comprised of a porous or non-porous support to which ligands have been immobilized, wherein said ligands comprise at least one aliphatic sulfamide. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulins, such as IgG, Fab fragments, fusion proteins containing immunoglobulins etc, by adsorption to a separation matrix that comprises the aliphatic sulfamide ligands of the invention.

Abstract: The present invention relates to the fabrication of a grafted, UV photo-polymerized silica-based monolithic column and the use of such column for the extraction of DNA. In one embodiment, a method is provided for fabricating a silica-based monolithic column, wherein a vessel is filled with a polymerization mixture that is formed into monolithic solid phase for DNA extraction through in situ photo-polymerization.

Abstract: A chromatography column is prepared with a stationary phase comprising a deuterated poly(ethyleneglycol), or other deuterated polymer. Formation of the stationary phase can be performed using exactly the same methodology as used when forming a stationary phase with the equivalent non-deuterated polymer. The deuterated poly(ethyleneglycol), or other deuterated polymer, preferably has increased thermal stability as compared to non-deuterated poly(ethyleneglycol), or equivalent non-deuterated polymer. This reduces bleeding of the stationary phase during gas chromatography and allows the use of greater operating temperatures.

Abstract: A method for controlling fluid flowing through a chromatographic system includes determining a fluidic parameter related to density at a first fluidic location in the chromatographic system; and in response to the determined fluidic parameter, modifying a volumetric flow rate or a pressure at a second fluidic location in the chromatographic system to produce a selected mass flow rate of the fluid.

Abstract: A non-particulate organic porous material with optical resolution capability, the non-particulate organic porous material having a continuous pore structure, which comprises macropores and mesopores, the macropores being interconnected forming mesopores with a radius of 0.01-100 ?m in the interconnected parts, and optically active groups uniformly introduced therein possesses high physical stability, can be used under wide separating conditions, and has a large capacity for separating optically active substances (enantiometers).

Abstract: Disclosed is a separating agent for optical isomer which is excellent in optical separation ability. Specifically disclosed is a separating agent for optical isomer containing: an inclusion complex including a ?-conjugated polymer in a polymer compound having a hydroxy group or an amino group; and a carrier, the inclusion complex being carried by the carrier.

Abstract: A plate apparatus for use in countercurrent chromatography is disclosed which comprises a disk shaped tube support (60) having a tube support pattern form in an upper surface (63) of the support and configured to accommodate at least one layer of fluid flow tubing (70), wherein the pattern comprises at least one spiral groove (62) and at least one return path (72).

Abstract: To provide a medium suitable for high speed/high resolution, rich in hydrophilicity and resistant to a high concentration aqueous alkaline solution. A medium comprising crosslinked polymer particles containing from 20 to 95 mol % of repeating units derived from a (meth)acryloyl monomer represented by the formula (1): wherein R2 is a hydrogen atom or a C1-4 alkyl group, R1 is —NR3-R4-R5 or —O—R4-R5, R3 is a hydrogen atom or a C1-4 alkyl group, R4 is a C6-15 alkylene group containing an alicyclic ring or a C4-8 linear alkylene group, and R5 is a halogen atom, an alcoholic OH group, an amino group, a glycidyl group or an epoxy group.

Abstract: In an embodiment, a porous composite particulate material includes a plurality of composite particles. Each composite particle includes an acid-base-resistant core particle at least partially surrounded by one or more layers of acid-base-resistant shell particles. The shell particles are adhered to the core particle by a polymeric layer. The shell particles and/or core particles may be made from an acid-base-resistant material that is stable in harsh chemical conditions. For example, the shell particles and/or core particles may be made from diamond, graphitic carbon, silicon carbide, boron nitride, tungsten carbide, combinations of the foregoing, or other acid-base-resistant materials. The porous composite particulate materials disclosed herein and related methods and devices may be used in separation technologies, including, but not limited to, chromatography, and solid phase extraction.

Abstract: The present invention concerns a method of purifying colistin using reverse phase chromatography, wherein loading a column with colistin base in acetic acid and high ethanol concentration and eluting with low ethanol concentration is performed.

Abstract: Disclosed are methods for the chromatographic separation of rebaudioside A from stevioside in glycoside solutions that are derived from stevia. The chromatographic separation may be an adsorb/desorb type of chromatographic separation or a fractionation type of chromatographic separation.

Abstract: The present invention is relates to method for production of a biomolecule adsorbent with a pentadentate ligand with very strong chelating properties, and to methods for use thereof for, for example, protein purification, detection or binding. The biomolecule adsorbent comprises the formula: wherein Q is a carrier, S is a spacer, L is an amide linkage, X is COOH, and n=2 or 3.

Abstract: The present invention relates to the use of a supramolecular system in order to remove particles from a liquid medium containing same. According to the invention, the supramolecular system includes at least one molecule having a low molecular weight and/or an organic compound from living organisms, preferably from jellyfish, said compound being selected from among collagen, a polysaccharide, a proteoglycan or a mixture of two of said organic compounds, and said molecule having a low molecular weight and formula (I) as defined herein. The invention also relates to a method for removing particles from a liquid medium containing same. The invention is particularly suitable for use in water decontamination and biotechnology.

Abstract: A multicapillary sample preparation device, especially useful for handling biological samples, comprising a plurality of uniform capillary tubes coated with a stationary phase, and arranged in a monolithic element. The multicapillary device is suitable for attachment to a pipette, micropipette, syringe, or other analytical or sample preparation instrument.

Abstract: Provided in one embodiment is a method of making an aerogel, comprising: (A) subjecting a suspension or a solution comprising a concentration of at least one chemical reactant to at least one of a hydrothermal and a solvothermal process for at least a reaction time to form an in-situ hydrogel, wherein the hydrogel comprises particulates having an asymmetric geometry, and (B) removing a liquid from the hydrogel to form an aerogel.

Abstract: Aimed at readily and exactly separate 8-hydroxy-2?-deoxyguanosine (8-OHdG) contained in a sample, a column packing material used for separating 8-OHdG, which contains a packing material composed of a material having a straight-chain hydrocarbon group having 6 or more and 30 or less carbon atoms as a functional group, and having a carbon content over the surface of carrier of 18% or less by element ratio, wherein the packing material contains 1 cumulative percent or more and 20 cumulative percent or less, on the particle-count basis, of particles having a circle-equivalent diameter, measured using a flow-type particle image analyzer, of 0.5 ?m or larger and 10 ?m or smaller, is used.

Abstract: The present is a hydrophilic polymer microparticle, which has a DW/DA ratio of 2.0 or less, the DW representing a particle diameter of the hydrophilic polymer microparticle dispersed in water, the DA representing a particle diameter of the hydrophilic polymer microparticle dispersed in acetone, each of the particle diameters measured by a particle size distribution analyzer after the hydrophilic polymer microparticle is dispersed in water or acetone, subjected to irradiation with ultrasonic waves for 15 minutes, and allowed to stand at a temperature of 25° C. for 240 hours for equilibration; and has a contact angle with water of 70° or less, the contact angle measured using a contact angle measurement apparatus at a temperature of 25° C. by forming a droplet of pure water on a single layer of the hydrophilic polymer microparticles arranged with no space therebetween.

Abstract: Provided are methods for determining the amount of reverse T3 in a sample using mass spectrometry. The methods generally involve ionizing reverse T3 in a sample and detecting and quantifying the amount of the ion to determine the amount of reverse T3 in the sample.

Abstract: The invention provides a preparation method of super-macroporous polymeric microspheres and a product thereof. The method is characterized in that adding high content of surfactant into an oil phase containing a monomer and dispersing the oil phase containing the monomer and the surfactant into an aqueous phase. Super-macroporous microspheres are obtained by means of suspension polymerization. In the interior of the microspheres, 10-60% of the pores are distributed in a range of 500-900 nm. The microspheres have a particle diameter of 1-200 ?m and a porosity of 30%-90%. The product is suitable for being used as a stationary phase matrix in liquid chromatography, a carrier for high performance catalysts and a high performance adsorbent, and especially, being used as a separation medium for separating biomacromolecules.

Abstract: A method includes passing a mobile phase fluid flow comprising liquefied CO2 through a separation column; then introducing a makeup fluid flow into the mobile phase fluid flow to form a mixed fluid flow; and then splitting the mixed fluid flow.

Abstract: Disclosed is a process for the separation of one or more fractions from a resulting reaction mixture of an enzymatically catalyzed polymerization of flavonoid using HPLC with a varying concentration of organic solvent in the mobile phase. The isolated fractions can be used in numerous applications including as an antioxidant.

Abstract: The present invention relates to a method for recovering an aqueous phase comprising biomolecules dissolved therein from a multiphasic mixture, comprising at least said aqueous phase and a further liquid phase which is immiscible with said aqueous phase wherein said further liquid phase comprises at least one hydrocarbon compound. The invention further relates to the use of a hydrophilic filtering material, a device comprising such a filtering material or a kit comprising said device for recovering an aqueous phase comprising biomolecules dissolved therein from a mixture of said aqueous phase and at least one hydrocarbon compound comprising further liquid phase which is immiscible with said aqueous phase.