Abstract: A method for manufacturing a packing material includes a first step of reacting an inorganic particle having a hydroxyl group with a silane coupling agent including a silane coupling agent having an alkenyl group with a carbon number of 2 or more and 8 or less and/or an alkynyl group with a carbon number of 2 or more and 7 or less and a second step of reacting the inorganic particle having reacted with the silane coupling agent, with a compound represented by a general formula of: (in the formula, R1 is an alkyl group with a carbon number of 4 or more and 50 or less or an aryl group with a carbon number of 6 or more and 30 or less and each of R2 and R3 is independently a hydrogen atom, a chloro group, or an alkyl group with a carbon number of 1 or more and 4 or less.).

Abstract: An injection device (10) includes a carrier inlet (40), a sample inlet (46), waste outlet (44) and a chamber outlet (64) attached to separation column (66). Valves (52, 54, 56) are used to control flow such that sample flows into chamber (22) and is carried into the chamber outlet (42).

Abstract: The invention provides columns and methods for the purification and concentration of an analyte (e.g., a biological macromolecule, such as a peptide, protein or nucleic acid) from a sample solution. The columns typically include a bed of extraction medium positioned in the column between two frits. In some embodiments, the extraction columns employ modified pipette tips as column bodies. The invention also provides methods for purifying and concentrating multiple analytes simultaneously.

Abstract: The present invention relates a method of preparing agarose beads, which method results in a population of beads which are of relatively uniform particle size. In an advantageous embodiment, the beads are of a particle size less than 10 ?m, and the coefficient of variation C.V. of the population is less than 15%. The beads according to the invention are advantageously used in biological separation methods, such as in the production of chromatographic packing materials; drug carriers; or in any method of biological engineering.

Abstract: The present invention relates to liquid clarification. More closely, the invention relates to beverage clarification, such as reduction of colloidal (not microbial) haze-causing substances in beer or related beverages such as wine, juices, flavorings etc. The method of the invention uses a hydrophilic surface for adsorption of haze-forming substances by hydrogen bonding interaction properties between the surface and the haze-forming substances.

Abstract: The invention is a method for the purification of mono-PEGylated erythropoietin using two cation exchange chromatography steps wherein the same type of cation exchange material is used in both cation exchange chromatography steps and a method for producing a mono-PEGylated erythropoietin in substantially homogeneous form.

Abstract: The present invention is directed to inorganic oxide particles that have been modified with a chelator group. In an embodiment, the chelator group is covalently bonded to a polymer. In an embodiment, the chelator group is covalently bonded to a chiral selector ligand. The present invention is also directed to methods of modifying inorganic oxide particles with a chelator group.

Abstract: Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (such as Protein A or Protein G affinity column) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities in biological samples even at high conductivities and pH.

Abstract: The present invention relates to a process for separating a target radiolabelled compound from an impurity, apparatus for performing such a process and a removable cassette for use in such apparatus. Also provided are methods for using the target radiolabelled compound obtained by a method comprising the separating process of the invention.

Abstract: A process for producing a chromatographic stationary phase for use in reversed-phase chromatography. Chromatographic stationary phases prepared according to the methods of the current invention and liquid chromatography columns, which include the stationary phases, are also provided.

Abstract: A process for separating a feed F by simulated moving bed adsorption in a SMB device comprises external bypass lines Li/i+1 which directly connect two successive plates, Pi, Pi+1, the index “i” being either even or (exclusive of the foregoing) odd, along the whole length of the column, allowing said plates to be flushed, in which each of the bypass lines Li/i+1 comprises automated means for regulating the flow rate in the bypass lines, the bypass lines in certain cases being subject to a flushing flow during injection or withdrawal operations.

Abstract: A process for separating a feed F by simulated moving bed adsorption in a SMB device comprises at least one zone 1 for desorption of the compounds produced in the extract, a zone 2 for desorption of the compounds produced in the raffinate, a zone 3 for adsorption of the compounds produced in the extract, a zone 4 located between the raffinate withdrawal and the desorbant supply, the device comprising external by-pass lines Li/i+1 directly connecting two successive plates Pi and Pi+1; in which the degree of opening of means for restricting the flushing flow rate of the by-pass lines Li/i+1 are sequentially modified such that: 1) in an operational zone where there is at least one closed by-pass line, a super-synchronicity of the flushing flow rate is established in all of the by-pass lines which are not closed belonging to the zone under consideration, said super-synchronicity being defined by the following formula: S=a+b(nf/nt) in which the constant a is a constant in the range ?5 to 5 and b is a constant

Abstract: A process for separating a feed F by simulated moving bed adsorption in a SMB device is described, the device comprising external bypass lines Li/i+1 which directly connect two successive plates Pi, Pi+1, the index i being either even or (exclusive of the foregoing) odd, along the whole length of the column, in order to flush said plates, in which each of the bypass lines Li/i+1 comprises automated means for regulating the flow rate in the bypass lines, the degree of opening of said regulating means being defined by the following three rules: a) establishing a flow rate corresponding to an oversynchronicity in the range 15% to 30% in all of the open bypass lines of zone 1; b) establishing a flow rate corresponding to the synchronicity to within plus or minus 8% in all of the open bypass lines of zones 2 and 3; c) establishing a flow rate corresponding to an oversynchronicity in the range 20% to 40% in all of the open bypass lines of zone 4.

Abstract: The present technology relates to methods and systems for the removal of pathogens and fragments thereof in hemodialysis patients. In particular, methods and systems are described where lectins can be used to remove the Hepatitis C virus and fragments thereof in hemodialysis patients, and to provide a sustained reduction in viral load.

Abstract: Multi-layered macromolecules wherein the layers are covalently bonded together and wherein the macromolecules are covalently bonded to solid particulate substrates, methods for the preparation of such compositions, and methods for their uses in a multitude of end use applications ranging from the purification of waste chemical and metal process streams to the separation and identification of proteins, peptides, and oligionucleotides.

Abstract: The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilized, wherein said ligands comprise at least one sulphonamide and the R group of the sulphonyl comprises an aromatic group. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to the separation matrix.

Abstract: The present invention relates to a separating agent for solid-phase extraction which comprises core particles of a base material; a hydrophilic polymer layer formed on the surface of the particles; and a hydrophobic polymer layer formed on the surface of the hydrophilic polymer layer. The separating agent can be used in a cartridge or a column for solid-phase extraction and the latter can in turn be used in a variety of methods such as a method for concentrating a subject to be separated; a method for removing impurities; a method for solid-phase extraction of an environmental, medicinal and/or biological samples; and a method for pre-treating a protein component-containing sample.

Abstract: An ion chromatography apparatus comprising: (a) a first chromatography column, (b) a second chromatography column, the volume of the second column being no greater than 0.9 times the volume of the first; and (c) valving disposed between said first and second columns permitting selective transfer of separated ionic species from first chamber to second chamber for further analysis.

Abstract: The present invention relates to a separation matrix comprised of a support to the surfaces of which polymer chains have been coupled, wherein each polymer chain presents recurring proton-donating groups and at least the surface of the support is substantially hydrophilic. In the most advantageous embodiment, the support is porous cross-linked agarose, the polymers are poly(acrylic acid) and the proton-donating groups are carboxyl groups. The matrix is useful e.g. to remove PEG from pegylated and/or native compounds in a liquid. Accordingly, the invention also encompasses a method, such as a chromatographic method, wherein the separation matrix according to the invention is used, for example as a pre-treatment of a reaction mixture that comprises unreacted PEG, pegylated proteins and native proteins.

Abstract: The present invention relates to a method of preparing a library of resins which are useful in chromatography, which method comprises creating a diversity of multi-modal ion exchange resins; and providing the diversity in a parallel system in which each resin is presented separated from the other resin(s).

Abstract: The invention relates to a method for separating constituents of a liquid feed by liquid-liquid centrifugal chromatography and to a device for implementing it. Constituents (A, B) having different partition coefficients such that they are respectively carried along at unequal velocities by a light solvent (l) and a heavier solvent (L), the feed is continuously injected at an intermediate point of a separation column (col), successive cycles comprising alternately a heavy solvent injection stage and a light solvent injection stage are carried out respectively at the two ends of the column consisting of the interconnection in series of at least one set of separation cells, and the separated solvents (FA, FB) are collected during each one of said stages at the column ends opposite the solvent injection points.

Abstract: The present invention is to provide a method of producing a separating agent for an enantiomeric isomer having a high optical resolution power together with sufficient solvent resistance. That is, a method of producing a separating agent for an enantiomeric isomer comprising the steps of allowing the porous carrier to carry the optically active polymer compound by bringing the porous carrier into contact with a dope of the optically active polymer compound and exposing a product to radiation.

Abstract: The present invention provides germania-silica-based sol-gel monoliths that form the solid-phase material for extraction, isolation, preconcentration, and separation of analytes. In one embodiment, the germania-silica-based sol-gel monolith can be used to coat surfaces of the inner walls of extraction and chromatographic columns. In a preferred embodiment, the sol-gel germania-silica monolith of the present invention is formed from hydrolysis and polycondensation of precursors comprising tetraethoxygermane, tetramethoxysilane, and polyethylene glycol. Also provided are methods of preparing the germania-silica-based sol-gel monolith of the present invention.

Abstract: A method of preparing a sol-gel monolithic column includes the step of forming a separation bed (14) from a sol-gel solution in a single process step. This column has improved characteristics for CEC based on its incorporated surface charge and ease of operation due to a lack of or need for end frits. Also, a second type of column includes an optical window (30) for on-column detection.

Abstract: Described is an affinity microcolumn comprising a high surface area material, which has high flow properties and a low dead volume, contained within a housing and having affinity reagents bound to the surface of the high surface area material that are either activated or activatable. The affinity reagents bound to the surface of the affinity microcolumn further comprise affinity receptors for the integration into high throughput analysis of biomolecules.

Abstract: Disclosed is a boron removal method which can remove boron from boron-containing water satisfactorily by using a small amount of a chemical agent, and which generates small amount of precipitate. Also disclosed is a boron-removing agent which is easy to handle and has excellent boron-adsorption ability. A xerogel of an organic polymeric compound having a cis-type diol structure and a water-soluble salt of an alkaline earth metal are added to a boron-containing water under alkaline conditions.

Abstract: Pretreatment method and apparatus to remove matrix ions from a liquid sample, prior to separation of the sample analytes (e.g. by chromatography), by flowing the liquid sample into a sample compartment and stopping the flow. The sample compartment has a wall comprising an ion exchange membrane having exchangeable ions of the same charge as the matrix ions to be removed. A regenerant liquid stream flows through a regenerant flow compartment on the other side of the membrane from said parked liquid sample. Matrix ions in the parked liquid sample are transported across the membrane into the regenerant flow compartment. Suppression may be performed electrolytically and/or chemically. A concentrator column may also be used.

Abstract: A process for the chromatographic interseparation of rhodium in admixture with at least one base metal from a feed solution includes complexes of these metals. The process includes contacting the solution with at least one chromatographic medium, and eluting one or more fractions such that at least one fraction includes rhodium complex substantially free from base metal contamination. The at least one chromatography medium includes a support functionalized with substituted amine groups which further include at least one hydrophilic group. Other platinum group metals can also be separated, both from one another and from base metals.

Abstract: Embodiments of the present invention relate to a UV-curable polyurethane-methacrylate (PUMA) substrate for manufacturing microfluidic devices. PUMA is optically transparent, biocompatible, and has stable surface properties. Embodiments include two production processes that are compatible with the existing methods of rapid prototyping, and characterizations of the resultant PUMA microfluidic devices are presented. Embodiments of the present invention also relate to strategies to improve the production yield of chips manufactured from PUMA resin, especially for microfluidic systems that contain dense and high-aspect-ratio features. Described is a mold-releasing procedure that minimizes motion in the shear plane of the microstructures. Also presented are simple yet scalable able methods for forming seals between PUMA substrates, which avoids excessive compressive force that may crush delicate structures. Two methods for forming interconnects with PUMA microfluidic devices are detailed.

Abstract: Systems, methods and compositions for the purification, separation and analysis of anionic compounds, including polyanionic compounds are disclosed.

Abstract: A liquid chromatographic system is provided including catalytically combining hydrogen and oxygen gases in the chromatography eluent stream in a catalytic gas elimination chamber, to form water and thereby reduce the gas content in the eluent stream. Also, a liquid ion chromatographic system in which the effluent from the detector is recycled to a membrane suppressor and then is mixed with a source of eluent for recycle to the chromatographic column.

Abstract: This invention relates to a pipette tip or tube designed to perform biological or chemical sample preparation, purification or separation. The narrow end of the pipette tip is twisted in such a way that it forms a narrow spiral passage, which allows the retention of the chromatographic material in the pipette tip. The narrow spiral formed by twisting the capillary or the tube creates an opening that is large enough to permit the passage of fluids or smaller particles, but small enough to block the passage of solid materials or larger particles, The device described herein can be used for sample separation or purification using a vacuum, pressure, centrifugation, gravitation or other separation methods.

Abstract: An ion-exchange chromatography support for reducing the ADAMTS13 amount present in a plasma-derived solution containing human von Willebrand factor. The support includes a large-pore, vinyl polymer-type resin bearing DEAE groups, and a buffer including trisodium citrate, sodium chloride, calcium chloride, glycine and lysine.

Abstract: A suppressed ion chromatographic apparatus using a regenerant recycle loop, comprising (a) an ion separation device, (b) a membrane suppressor, (c) a detector, (d) a container for regenerant solution, (e) a first conduit between the ion separation device and the suppressor, (f) a second conduit between the regenerant solution container and the suppressor, (g) a third conduit between the suppressor and the regenerant solution container, and (h) a regenerant solution recycle loop out of fluid communication with the detector outlet.

Abstract: Provided herein are methods of liquid column chromatography in which preparative chromatography is performed in-line with analytical chromatography. In particular aspects a monolithic preparative column is used to purify an analyte of interest from a mixture of other substances by applying the mixture to the column, reversing the flow through the column to elute the analyte, which is applied to an analytical column provided in-line with the preparative column. In other aspects, a single monolithic column is used to perform both the preparative chromatography and analytical chromatography steps in succession. In another aspect, a chromatography system is provided to perform preparative and analytical chromatography using a single monolithic column.

Abstract: Provided are a stationary phase and a column using a cucurbituril-bound silica gel, and a method of separating taxane using the column. The taxane separation method includes: preparing a column for taxane separation, the column being packed with a stationary phase including a cucurbituril-bound silica gel in which a cucurbituril represented by Formula 1 or 2 is covalently bound to a modified silica gel represented by Formula 3; dissolving a taxane powder in a solvent to prepare a taxane-containing solution; applying the taxane-containing solution to the column; supplying a mobile-phase solvent to the column to obtain a taxane extract from the column; and purifying taxane from the taxane extract. Therefore, highly purified taxane can be separated from a low-purity crude taxane extract.

Abstract: A liquid chromatography apparatus is provided with a sample preparation unit, a column that separates components of a sample, an eluent supplier that includes a feeder for supplying eluents to the column, a flow path directional valve capable of introducing fixed amounts of the sample and the eluents to the column, an analyzer for analyzing a test solution composed of the sample components separated by the column and one of the eluents, and a controller, wherein the eluent supplier supplies the eluents to the flow path directional valve in an unmixed state. As a result of employing this configuration, analysis time is shortened and eluent consumption is reduced.

Abstract: This invention relates to a breakthrough in the art of chromatography allowing 1) larger scale of operation; 2) faster processing time; 3) disposability; 4) reduction of media/resin expenses; and 5) a reduction of capital equipment investment. In this invention, the chromatography column is replaced by a module that consists of two or more interconnected tangential flow filters and static mixers. The chromatography resin flows through this module in a single pass, while similar operations to a regular chromatographic process are performed on the resin (binding, washing, elution, regeneration, and equilibration). The buffers for these operations are pumped into the module in a countercurrent direction to the flow of resin, and permeate solutions from later stages are recycled back into previous stages. This creates concentration gradients in the permeate solutions of the tangential flow filters in the countercurrent direction to resin flow, thus saving buffer volume and increasing process efficiency.

Abstract: The present invention relates to a method for generating at least one polydentate metal chelating affinity ligand, which method comprises the steps of (a) providing at least one scaffold defined by the general formula (I): H2N—(X1)n—S—S—(X2)m—CH2—NH2 wherein X1 and X2 irrespective of each other are carbon atoms or heteroatoms, and n and m irrespective of each other are integers of 1 to 5; (b) providing at least one polydentate metal chelating affinity ligand arm, optionally in a form wherein the metal chelating functionalities of at least one arm are protected, on each scaffold by derivatisation of the nucleophilic NH2 groups of the scaffold; (c) reducing the disulfide bond of the derivatised scaffold; and, if required (d) deprotecting the functionalities of the ligand arm(s) provided in step (b). In the most preferred embodiment, the reduction of the disulfide bond and the deprotection step is performed essentially simultaneously.

Abstract: Disclosed herein is a suppressor using one ion-exchange tube which has an inner diameter close to that of a tube connected to a separation column and which is constituted by an ion-exchange membrane. The ion-exchange tube is folded or wound more than once in a plane into a sheet form to provide an ion-exchange tube sheet through which an eluate from a separation column flows. The ion-exchange tube sheet is accommodated in a container. The container provides a regenerant flow channel so that a regenerant is allowed to flow on both sides of the ion-exchange tube sheet.

Abstract: The present invention relates to a process of enriching element content in stable isotopes of light elements in ion-exchange chromatography. The process comprises development of band of light elements in a chromatographic column; elution of said band in a discontinuous basis so as to disconnect said chromatographic column at particular stage; and finally the process comprises of regeneration step.

Abstract: A process for adsorption-based separation of bioparticles from an aqueous suspension containing such bioparticles is disclosed which process comprises a) bringing said aqueous suspension into contact with a macroporous cryogel monolith carrying on its surface a ligand capable of interacting specifically or non-specifically with a receptor on the surface of a bioparticle to be separated to adsorb said bioparticle to said macroporous cryogen monolith; and b) liberating said bioparticle from said macroporous cryogel monolith by elution, wherein said macroporous cryogel monolith is subjected to a physical deformation in conjunction with exposure to eluting medium.

Abstract: A sample concentrator for concentrating analytes in a solvent-containing liquid sample stream, including concentrator housing having a sample stream flow channel and a gas stream flow channel having an inlet and an outlet, a heater for gas in the gas stream conduit, and a hydrophilic ion exchange or non-ionic membrane barrier separating said gas stream flow channel and said sample stream flow channel. Solvent is evaporated from the liquid sample stream in said sample stream flow channel in or at the interface with said membrane, when the gas stream is at an elevated temperature. A regeneration step is used to regenerate the ion exchange membrane barrier.

Abstract: A multicapillary column especially useful for liquid chromatography and sample preparation comprising a plurality of uniform capillaries coated with an insoluble stationary phase, wherein the thickness of the stationary phase is correlated with the radius of the individual capillaries for high efficiency.

Abstract: The present invention relates to a chromatographic separation process for fractionating solutions containing sugars, sugar alcohols, sugar acids and/or betaine by utilizing fractions comprising parts of the separation profile as a substituent of the eluent in a novel and inventive manner. The starting solutions are typically multicomponent plant-based extracts and hydrolysates, stillages, and fermentation products and derivatives thereof. The process prow vides improved separation capacity and separation efficiency without affecting the yield or purity of product components, such as sugars, sugar alcohols, sugar acids, and betaine. The process can especially be applied to sequential SMB separation systems.

Abstract: Granulated products are provided and include carbonaceous particles and a carbonized agent or binder. The agent or binder is preferably a synthetic resin, pitch component, or mixture thereof. Packing materials for packing columns used in chromatographic separations are also provided as are methods of chromatographic separation using the materials. In addition, methods are provided to provide a variety of different types of carbonaceous products. A variety of chemical groups can be, prior to heat-treatment and/or thereafter, attached to the granules to form modified granules.

Abstract: The present invention is a system for continuous, single-pass countercurrent tangential chromatography having multiple single-pass modules. A single-pass binding step module is used for binding product from an unpurified product solution with a resin slurry. A single-pass washing step module is used for washing impurities from the resin slurry. A single-pass elution step module for eluting an output of the washing step module is used as purified product solution. A single-pass regeneration step module is used for regenerating the resin slurry. In the current invention, the resin slurry flows in a continuous, single-pass through each of the single-pass modules, and one or more of the single-pass modules comprise two or more stages with permeate flow directed countercurrent to resin slurry flow within that single-pass module. The present invention replaces conventional column chromatography and may be made disposable.

Abstract: A method of preparing a sol-gel monolithic column includes the step of forming a separation bed (14) from a sol-gel solution in a single process step. This column has improved characteristics for CEC based on its incorporated surface charge and ease of operation due to a lack of or need for end frits. Also, a second type of column includes an optical window (30) for on-column detection.

Abstract: A composite sorbent material is disclosed which comprises A) a housing of a dense, insoluble material having openings therein and B) a porous polymer gel housed within said housing, said housing being provided with openings therein for fluid communication between said porous monolithic polymer gel and the surroundings, and said porous monolithic polymer gel occupying the inner space of said housing and being kept on place within said housing by mechanical means. Processes for the preparation of composite sorbent materials by cryoprecipitation are also disclosed as well as the use of the composite sorbent material according to the invention e.g. for the removal of pollutants from gases or liquids, for the enrichment or separation of molecules and for cell culture.

Abstract: Embodiments of the present invention provide a method of chromatographic delipidation comprising separating a lipid-containing sample on a superficially porous stationary phase at greater than about 70° C., at least about 80° C., having at least one mobile phase comprising an ion-pairing agent in water, an ion-pairing agent in an organic modifier, an acid in an organic modifier, and an alcohol. The invention provides minimal protein losses and high run-to-run reproducibility. The on-column delipidation method aventageously utilize reversed phase liquid chromatography.