Abstract: The subject matter of the invention is an innovative method for the separation of polymer systems regarding their molecular weight, chemical structure, chain architecture, and colloidal additives. Such separations are currently accomplished by selective precipitation from solution, by a fractionated crystallization also from solution, and by means of gel chromatographic methods. The invention pertains to a separation of polymer systems by means of permeation through polymer films—semi-crystalline, cross-linked, amorphous—with thicknesses in a nanometer scale. The restriction to thicknesses in a nanometer scale is essential for a high throughput of polymers. Of particular significance is the selectivity towards colloidal additives with a structure that is not in chain form.

Abstract: The present invention relates to a process for extracting constituents from organic material, comprising the step of extracting with a monophasic mixture of 50% by volume to 90% by volume of methanol and 50 to 10% by volume of water and optionally 0% by volume to 40% by volume of a further solvent or solvent mixture.

Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.

Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.

Abstract: Disclosed herein are an automated simultaneous separation system for radionuclides in multiple samples and a method for automatically separating uranium (U) using the same, which can automatically and simultaneously separate multiple samples. The automated simultaneous separation system includes: a solution distributor(30) for distributing a separation solution in seven directions; independent columns (80) of the same number as that of the samples, the columns (80) containing resin for chemical separation; a 8-channel tubing pump (60) for separately injecting the samples into the columns (80); first and second 3-way solenoid valves (50, 90) mounted at the front end and the rear end of the columns (80), and a digital pump speed controller (70) for freely controlling injection speed of the samples and solutions according to separation steps.

Abstract: A porous substrate capable of adsorptive filtration of a fluid having a porous self-supporting substrate and one or more porous, adsorptive coatings comprising from about 1 to about 80% of the void volume of the pores of the substrate. The resultant substrate has good convective and diffusive flow and capacity. The substrate may be crosslinked, have one or more capture chemistries attached to it and is useful as a chromatography media for the selective filtration of desired species including biomolecules such as proteins and DNA fragments.

Abstract: A porous substrate capable of adsorptive filtration of a fluid having a porous self-supporting substrate and one or more porous, adsorptive polymeric coatings comprising from about 1 to about 80% of the void volume of the pores of the substrate. The resultant substrate has good convective and diffusive flow and capacity. The substrate may be cross-linked, have one or more capture chemistries attached to it and is useful as a chromatography media for the selective filtration of desired species including biomolecules such as proteins and DNA fragments.

Abstract: Provided is a separating agent for enantiomeric isomers that is advantageous from the production standpoints of having a high enantiomeric resolution ability inherent in polysaccharide derivatives, solvent resistance, high solubility in a reaction solvent, and good filtration property. That is, the separating agent for enantiomeric isomers includes a polysaccharide oligomer derivative derived from a polysaccharide oligomer having a number-average degree of polymerization of from 5 to less than 50, supported on a carrier.

Abstract: Ultraporous sol gel monoliths and methods for preparing the same are provided, having superior flow characteristics for chromatography and analytical chemistry applications. The methods for forming an ultra porous sol-gel monolith include (a) forming a solution comprising a porogen, a matrix dissolving catalyst and a sol gel precursor; (b) allowing the solution to form a gel; and (c) drying the gel at an elevated temperature. The ultraporous sol gel monoliths are characterized by a porosity of up to about 97%, a BET surface area of at least about 50 m2/g and substantially no micropores.

Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.

Abstract: The invention relates to a process for the recovery of a macrolide in substantial pure form including: a) treating an impure or crude macrolide with water immiscible solvent to form a mixture, b) optionally concentrating the mixture, c) treating with ammonia gas to phase out impurities, d) separating impurities, e) optionally concentrating a phase containing the macrolide, f) loading on silica gel chromatography, optionally reversed phase or pretreated with silver, and eluting the macrolide, g) affording the macrolide in the substantially pure form, h) optional repetition of steps f and g to afford the macrolide in the substantially pure form. The macrolide is preferably tacrolimus, immunomycin or sirolimus.

Abstract: A suppressed ion chromatographic apparatus using a regenerant recycle loop, comprising (a) an ion separation device, (b) a membrane suppressor, (c) a detector, (d) a container for regenerant solution, (e) a first conduit between the ion separation device and the suppressor, (f) a second conduit between the regenerant solution container and the suppressor, (g) a third conduit between the suppressor and the regenerant solution container, and (h) a regenerant solution recycle loop out of fluid communication with the detector outlet.

Abstract: Permeable polymeric monolithic materials are prepared in a plastic column casing. In one embodiment, the permeable polymeric monolithic materials are polymerized by the application of heat from an external source starting at a low temperature such as 40 degrees centigrade, depending on the mixture and size of the column, and continuing at a higher temperature, such as 60 degrees centigrade. The temperature at the start of the polymerization is low enough so as not to cause exothermal run-away conditions and to avoid high heat of reaction that would prevent a substantially constant temperature across the cross-section of the column. The higher temperature is used after sufficient monomer depletion has occurred and steric interference has increased so the polymerization reaction is sufficiently slow to avoid heat of reaction generation high enough to cause significant reduction in the homogeneousness of the pore sizes.

Abstract: Wastewater, in the form of a colloidal solution containing microparticles, is filtrated using a second filter, having a gel film that is formed by suction on the surface of a first filter. The suction pressure in this process is made extremely weak to maintain the filtration capacity while extending intervals prior to clogging of second filter. Furthermore, the gel that becomes adsorbed onto the surface of second filter can be separated readily from the surface of second filter by stopping the suction and filtration can be continued again thereafter.

Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.

Abstract: Simultaneous sample purification, enrichment and analysis of pharmaceuticals, illicit drugs, pollutants, biotechnological products, synthetic organic reaction products and food/flavor ingredients from complex matrices can be performed using porous hollow fiber or porous-disk liquid-membrane devices. The devices are part of a multi-well (e.g. 96-well) plate. The devices can be used for selective separation and enrichment of complex mixtures containing trace levels of analytes, and can be used in tandem with analytical instruments which routinely handle multiple samples under high throughput screening conditions. A multi-well/multi-vial plate can into state-of-the-art HPLC or GC sampling systems or LC/MS or GC/MS instruments. Samples can be enriched several orders of magnitude and can directly be withdrawn from the fiber and injected into the chromatographic instruments. Alternatively, these enriched samples can be introduced directly into MS, CE or other detection devices.

Abstract: The present invention provides novel silicon compounds, methods for making these novel silicon compounds, compositions comprising these novel silicon compounds attached to substrates, methods for attaching the novel silicon compounds to substrates and methods for using the compositions in a variety of chromatographic applications.

Abstract: The present invention relates to a method of isolating target compounds from a liquid, which method including the steps of providing a mobile phase, which contains at least one target compound and wherein the conductivity corresponds to ?0.6 M; contacting the mobile phase with a separation matrix including one or more sulphonamide groups to adsorb one or more target compounds; contacting an eluent with the matrix to release target compound(s), wherein the conductivity of the eluent is reduced as compared to the mobile phase conductivity and the pH is substantially equivalent to the mobile phase pH; and, optionally, recovering at least one target compound or a purified liquid.

Abstract: Provided are a stationary phase and a column using a cucurbituril-bound silica gel, and a method of separating taxane using the column. The taxane separation method includes: preparing a column for taxane separation, the column being packed with a stationary phase including a cucurbituril-bound silica gel in which a cucurbituril represented by Formula 1 or 2 is covalently bound to a modified silica gel represented by Formula 3; dissolving a taxane powder in a solvent to prepare a taxane-containing solution; applying the taxane-containing solution to the column; supplying a mobile-phase solvent to the column to obtain a taxane extract from the column; and purifying taxane from the taxane extract. Therefore, highly purified taxane can be separated from a low-purity crude taxane extract.

Abstract: A system and method for integrating microfluidic components in a microfluidic system enables the microfluidic system to perform a selected microfluidic function. A capping module includes a microfluidic element for performing a microfluidic function. The capping module is stacked on a microfluidic substrate having microfluidic plumbing to incorporate the microfluidic function into the system. An infusion pump for delivering a fluid from a fluid source may be integrated in a microfluidic chip using a capping module having pumping components formed therein.

Abstract: The present disclosure relates to a system and method for protein separation including: fractionating a mixture of proteins on a reversed-phase superficially porous stationary phase at a temperature of greater than or equal to about 40° C. to recover a protein in from about 70 to 100 weight percent of the mixture of proteins.

Abstract: Embodiments of the present invention are directed to porous resins for solid phase extractions. The resins feature at least one hydrophobic component, at least one hydrophilic component and at least one ion exchange functional group. The resins exhibit superior wetting and ion exchange performance.

Abstract: An affinity matrix for use in affinity based molecular pull down and immunoprecipitation procedures. The affinity matrix comprises a polymeric support, a dye attached to a fraction of the polymeric support to enable optical detection of the polymeric support, and an affinity ligand other than the dye attached to a fraction of the polymeric support for the capture of a molecule. Also provided is a method for the isolation of a biomolecule from an aqueous solution. The method comprises combining the aqueous solution with an affinity matrix comprising a polymeric support and separating the affinity matrix from the aqueous solution. A dye is attached to a fraction of the polymeric support which enables optical detection and monitoring of the affinity matrix and, accordingly, reduces the likelihood of the loss of affinity matrix during the separation step. In addition, an affinity ligand other than the dye is also attached to a fraction of the polymeric support for the capture of the biomolecule.

Abstract: A method of regenerating N-Methyl-D-glu-camine-functional resin that has been used for boron-removal uses a closed recirculating loop for treating the conjugate acid salt of the N-Methyl-D-glucamine functionality of the resin. The new method reduces rinse water demand and improves pH control in a water treatment system. The new method can be used to improve the performance of boron-selective resins in stand-alone systems or as a second stage in a reverse osmosis seawater desalination system. The regeneration method is useful in any application where weakly basic anion exchange resin in the conjugate acid salt form is to be regenerated (neutralized) by alkaline treatment. Possible end use applications are in drinking water processing, agricultural water treatment, sweetener production, waste water processing, mining hydrometallurgy, and condensate polishing.

Abstract: The present invention relates to a method of manufacturing a chromatography matrix comprising providing a polysaccharide carrier comprising available hydroxyl groups; and reacting said hydroxyl groups with vinyl sulphonate to provide a sulphonate-functionalized (S-functionalized) cation exchanger. The hydroxyl groups of the carrier may be hydroxyls of the agarose polymer; or alternatively they may be provided on extenders such as polyhydroxyfunctional polymers. In one embodiment, the carrier is made of agarose with improved flow pressure properties.

Abstract: A for preparing Tyrosol and/or Hydroxytyrosol from oil mill wastewaters. includes: a) Microfiltration (MF), Ultrafiltration (UF). Nanofiltration (NF) and Reverse Osmosis (RO) of the OMW; b) Separation of Tyrosol, Hydroxytyrosol and other phenolic compounds from the concentrated RO; c) Oxidation of the obtained Tyrosol to Hydroxytyrosol in the presence of methyl rhenium trioxide and of hydrogen peroxide in a protic solvent.

Abstract: The invention relates to a method for separating constituents of a liquid feed by liquid-liquid centrifugal chromatography and to a device for implementing it. Constituents (A, B) having different partition coefficients such that they are respectively carried along at unequal velocities by a light solvent (1) and a heavier solvent (L), the feed is continuously injected at an intermediate point of a separation column (col), successive cycles comprising alternately a heavy solvent injection stage and a light solvent injection stage are carried out respectively at the two ends of the column consisting of the interconnection in series of at least one set of separation cells, and the separated solvents (FA, FB) are collected during each one of said stages at the column ends opposite the solvent injection points.

Abstract: The present invention relates to a method for separating products from a mixture by reversed phase high performance liquid chromatography using a column packed with a stationary phase which is hydrophobic and a mobile phase, wherein the loading step, during which the products to be separated are introduced in the column, is carried out with an aqueous phase while applying an overpressure of at least about 0.3 MPa.

Abstract: Process for the work-up of a mixture comprising ionic liquids and a further substance, wherein the substance present is separated off by means of adsorptive separation processes.

Abstract: The present invention is to provide a method of producing a separating agent for an enantiomeric isomer having a high optical resolution power together with sufficient solvent resistance. That is, a method of producing a separating agent for an enantiomeric isomer comprising the steps of allowing the porous carrier to carry the optically active polymer compound by bringing the porous carrier into contact with a dope of the optically active polymer compound and exposing a product to radiation.

Abstract: The present invention is directed to chromatographic media comprising a chromatographic absorbent as a stationary phase, wherein the chromatographic absorbent is disposed in the chromatographic media so as to provide a gradient of hydrophilic, hydrophobic, and/or ionic groups.

Abstract: The present invention provides novel silicon compounds, methods for making these novel silicon compounds, compositions comprising these novel silicon compounds attached to substrates, methods for attaching the novel silicon compounds to substrates and methods for using the compositions in a variety of chromatographic applications.

Abstract: Pretreatment method and apparatus to remove matrix ions from a liquid sample, prior to separation of the sample analytes (e.g. by chromatography), by flowing the liquid sample into a sample compartment and stopping the flow. The sample compartment has a wall comprising an ion exchange membrane having exchangeable ions of the same charge as the matrix ions to be removed. A regenerant liquid stream flows through a regenerant flow compartment on the other side of the membrane from said parked liquid sample. Matrix ions in the parked liquid sample are transported across the membrane into the regenerant flow compartment. Suppression may be performed electrolytically and/or chemically. A concentrator column may also be used.

Abstract: The objectives of this invention are to provide a filler for separating optical isomers, capable of efficiently carrying out optical resolution of optical isomer mixtures, and to provide a process for separating optical isomers by a simulated moving bed chromatography utilizing the filler. This invention provides a filler made of a carrier supporting a specific amount of an optically active high molecular weight compound. Separation by the simulated moving bed chromatography using the filler is carried out under the condition that the capacity factors have specific values.

Abstract: The present invention relates to a method of preparing a porous cross-linked polysaccharide chromatography matrix, which comprises to provide an aqueous solution of a gellable polysaccharide, wherein part of the hydroxyl groups are substituted with groups which are not susceptible to nucleophilic attack; to provide essentially spherical droplets of the substituted polysaccharide solution; to form a gel of the substituted polysaccharide solution; and to cross-link the gel by adding a cross-linking agent. The invention also encompasses a chromatography column packed with a matrix so produced as well as use thereof in e.g. protein purification.

Abstract: The present disclosure relates to a system and method for membrane protein separation including: fractionating a membrane sample on a reversed-phase superficially porous stationary phase at a temperature of greater than or equal to about 40° C. to recover a protein greater than about 70 weight percent of the mixture of proteins.

Abstract: A method for analyzing the content of perchlorate in a sample matrix comprising the steps of extracting the sample matrix and applying it to a reverse phase column, followed by elution with a mobile phase. The reverse phase column optionally has a protein coating deposited thereon.

Abstract: Multi-layered macromolecules wherein the layers are covalently bonded together and wherein the macromolecules are covalently bonded to solid particulate substrates, methods for the preparation of such compositions, and methods for their uses in a multitude of end use applications ranging from the purification of waste chemical and metal process streams to the separation and identification of proteins, peptides, and oligionucleotides.

Abstract: Polymer fibers having a novel cross-sectional geometry are used as stationary phase materials for liquid chromatography separations. Fibers of 20 to 50 micrometer diameters have surface-channel structures extending their entire lengths. Bundles of fibers having this novel cross-sectional geometry are packed in columns. Different polymer compositions permit the “chemical tuning” of the separation process. Channeled fibers composed of polystyrene and polypropylene have been used to separate mixtures of polyaromatic hydrocarbons (PAHs), Pb-containing compounds and fatty acids. Use of channeled fibers allows a wide range of liquid flow rates with very low backing pressures.

Abstract: An affinity ligand-matrix conjugate comprises the matrix and conjugated thereto by the group Z, a ligand having general formula (I): wherein one X is N and the other X is N, CCL or CCn; A1 and A2 are each independently O, S or N—R1 and R1 is H, C1-6 alkyl, C1-6 hydroxyalkyl, benzyl or ?-phenylethyl; B1 and B2 are each independently an optionally substituted hydrocarbon linkage containing from 1 to 10 carbon atoms; D1 is H or a primary amino, secondary amino, tertiary amino, quaternary ammonium, imidazole, guanidine or amidino group; and D2 is a secondary amino, tertiary amino, quaternary ammonium, imidazole, guanidine or amidino group; or B2—D2 is —CHCOOH—(CH2)3-4—NH2; and p is 0 or 1. Such conjugates are useful for the separation, isolation, purification, characterization, identification or quantification of an endotoxin.

Abstract: The present invention relates to a method of separating sugars and sugar alcohols from each other. More particularly the present invention relates to the use of a weakly basic anion exchange resin in a chromatographic separation process. The advantage of the present invention compared with the prior art is that it is especially suitable for separating reducing sugars in acidic conditions as well as for example in weakly acidic conditions. The method using chromatographic separation comprises at least one step where a weakly basic anion exchange resin is used in a chromatographic column or in a part of a column.

Abstract: The object of the present invention is to provide a production process of a microfluidic device in which a porous resin layer, which is capable of optimally fixing a large amount of enzyme, antigen or other protein or catalyst on the inner surface of a minute channel of a microfluidic device without obstructing said channel, is formed at a uniform thickness on the surface of said channel. In the present invention, by preliminarily forming a porous resin layer having a large number of pores in its surface on a substrate, and forming an indentation having a porous resin layer on its bottom surface by using an activating energy beam-curable composition on said porous resin layer, followed by forming a channel by adhering a member serving as a cover to said indentation, a porous resin layer can easily be formed at a uniform thickness on the surface of said channel.

Abstract: A process for the chromatographic interseparation of rhodium in admixture with at least one base metal from a feed solution comprising complexes of these metals. The process comprises contacting the solution with at least one chromatographic medium, and eluting one or more fractions such that at least one fraction comprises rhodium complex substantially free from base metal contamination. The at least one chromatography medium comprises a support functionalised with substituted amine groups which further comprise at least one hydrophilic group. Other platinum group metals can also be separated, both from one another and from base metals.

Abstract: A chromatography cartridge and method of manufacturing same. A chromatography cartridge of the present invention can include a tubular housing having an open end and an inner surface, and a plug. The plug can be positioned within the open end of the tubular housing and can have an outer circumferential surface, a substantial portion of the outer circumferential surface being fused to the inner surface of the tubular housing. A method for manufacturing a chromatography cartridge can include coupling at least a portion of the outer surface of the plug to at least a portion of the inner surface of the tubular housing in response to rotating at least one of the plug and the tubular housing about the longitudinal axis with respect to the other of the plug and the tubular housing.

Abstract: A method of producing and purifying promethium-147 including the steps of: irradiating a target material including neodymium-146 with neutrons to produce promethium-147 within the irradiated target material; dissolving the irradiated target material to form an acidic solution; loading the acidic solution onto a chromatographic separation apparatus containing HDEHP; and eluting the apparatus to chromatographically separate the promethium-147 from the neodymium-146.

Abstract: A chromatographic medium including thioether ligands bound to a cross-linked polymer matrix. The polymer is an organic polymer and the medium is hydrophilic. A method of manufacturing the media is also described. The media are useful in the chromatographic separation of components from solution, and in particular for the interseparation of platinum group metals.

Abstract: The present invention relates to a method of preparing a separation medium starting with an aqueous solution of spherical and preferably functionalised primary particles of defined size, comprising the following steps: a) inverse suspension dispersing said aqueous solution of primary particles as droplets in oil; b) evaporation to remove said aqueous solution and fusion between particles to form spherical aggregates; c) size fractionation of aggregates from step b); and optionally d) repeating steps a) to c) an optional number of times to form progressively larger spherical aggregates. The invention also relates to a separation medium produced by this method. The present separation medium can be used in chromatography in the form of essentially spherical aggregates in packed or expanded bed columns or in the form of assembled aggregates on a support for filtration purposes.

Abstract: A liquid chromatographic system is provided including catalytically combining hydrogen and oxygen gases in the chromatography eluent stream in a catalytic gas elimination chamber, to form water and thereby reduce the gas content in the eluent stream. Also, a liquid ion chromatographic system in which the effluent from the detector is recycled to a membrane suppressor and then is mixed with a source of eluent for recycle to the chromatographic column.

Abstract: The present invention is directed to a novel method of analysis of high molecular weight proteins. More specifically, it is directed to a novel reversed-phase LC/MS method of analysis of high molecular weight proteins including antibodies.

Abstract: This invention relates to the application of hydrophobic interaction chromatography combination chromatography to the purification of antibody molecule proteins.The questions raised in reexamination request, 09/006,966 filed Mar. 12, 2004 have been considered and the results thereof are reflected in this reissue patent which constitutes the reexamination certificate required by 35 U.S.C. 307 as provided in 37 CFR 1.570(e), for ex parte reexaminations, or the reexamination certificate required by 35 U.S.C. 316 as provided in 37 CFR 1.997(e) for inter partes reexaminations.