Utilizing Rotating Column Patents (Class 210/657)
  • Patent number: 9028693
    Abstract: A plate apparatus for use in countercurrent chromatography is disclosed which comprises a disk shaped tube support (60) having a tube support pattern form in an upper surface (63) of the support and configured to accommodate at least one layer of fluid flow tubing (70), wherein the pattern comprises at least one spiral groove (62) and at least one return path (72).
    Type: Grant
    Filed: December 3, 2008
    Date of Patent: May 12, 2015
    Assignee: The United States of America, as represented by the Secretary, Department of Health and Human Services
    Inventor: Yoichiro Ito
  • Patent number: 8597509
    Abstract: The present invention describes a method for constructing a spiral tube support apparatus used in countercurrent chromatography, improvements to countercurrent chromatography tube support design, and methods of using the improved countercurrent chromatography apparatus. The spiral tube support apparatus may be constructed by a shape forming process such as a three dimensional printing process that in turn uses a laser sintering technique, and can be made out of any easily formed material. Shape changes on both the tube support and the top improve the performance of the tube support and ease of manufacturing. The improved tube support and new method of creation permit use in both micro or macro scale preparations and use in small or large molecule preparations. In particular, specific solvent systems are described that permit purification of proteins.
    Type: Grant
    Filed: November 18, 2009
    Date of Patent: December 3, 2013
    Assignee: CC Biotech LLC
    Inventors: Martha Knight, Thomas M Finn
  • Patent number: 8562840
    Abstract: A spin column device, which contains a rigid porous filter that retains its shape during centrifugation, chromatography methods using the device to isolate a desired substance, e.g., a biological molecule, from other substances in a mixture, and kits containing the device with one or more reagents for use in the method.
    Type: Grant
    Filed: May 2, 2012
    Date of Patent: October 22, 2013
    Assignee: Bioventures, Inc.
    Inventor: Elliott P. Dawson
  • Patent number: 8491793
    Abstract: A method of recording and monitoring the operation and status of devices operating to purify water employed in the operation of a dialysis clinic, by providing a software program that monitors water purification devices that include a water softener, brine tank and reverse osmosis machine. This computer program is a data logging system that enables the user to monitor and record the status and performance of water purification equipment that is housed and used in a dialysis clinic. The software program will be used by technicians that work in three shifts on a daily basis to ensure that the water being purified for dialysis treatment is within an acceptable range of purity. This software replaces a paper logging system and is more versatile in security, accuracy, function and reporting.
    Type: Grant
    Filed: March 17, 2011
    Date of Patent: July 23, 2013
    Inventor: Pradip Teredesai
  • Patent number: 8430126
    Abstract: The invention concerns a rotary distribution apparatus (30) for distributing process liquids, typically in ion exchange and chromatographic separation in the processing of sugar juice. The apparatus includes a core (2) into which fixed process liquid supply pipes (3) extend and annular fluid distribution chambers defined between the core and an outer shell (4) which rotates around the core. Rotating pipes (5) are provided which rotate with the shell and which are connected between selected distribution chambers and selected ports in a rotating indexing disc (6) which rotates, with the shell, relative to a stationary indexing disc (7). The arrangement is such that as the rotating disc rotates, different streams of process liquid streams fed to the rotating disc through the rotating pipes are introduced to fixed ports in the stationary disc. The latter ports are arranged to feed the streams selectively to selected destinations.
    Type: Grant
    Filed: March 5, 2009
    Date of Patent: April 30, 2013
    Assignee: Tongaat Hulett Limited
    Inventor: Craig Jensen
  • Patent number: 8425770
    Abstract: The present invention is directed to methods and compositions for separating and isolating target molecules. In particular, the present invention comprises devices, such as CCDs, that contain particles without the need for support structures. Chromatography separation techniques, including but not limited to, ion exchange, size separation, affinity chromatography, ion exclusion, ligand exchange, reversed phase and normal phase partitioning, are used in the CCD. Methods also include low, medium and high pressure liquid chromatography. Such methods can be used for analytical, semi-preparative processes, initial clarification, preparative filtration and process scale applications.
    Type: Grant
    Filed: March 25, 2008
    Date of Patent: April 23, 2013
    Assignee: KBI Biopharma, Inc.
    Inventor: Heath Herman
  • Patent number: 8293100
    Abstract: Apparatus and methods related to centrifugal liquid chromatography are described. An angular velocity can be simultaneously imparted to a large number of chromatographic enclosures. Via centrifugal forces, a mobile phase fluid including a sample can be driven through a stationary phase within the chromatographic enclosure to perform a chromatographic separation process on components of the sample. The use of centrifugation as a driving force can allow significantly smaller stationary phase particles to be employed as compared to high performance liquid chromatography (HPLC). Further, for an equivalent chromatographic separation process, the use of centrifugation can provide much greater separation efficiencies than HPLC.
    Type: Grant
    Filed: March 12, 2010
    Date of Patent: October 23, 2012
    Assignee: Terrasep, LLC
    Inventors: Robert R. Kerr, James D. Whitaker, Peter G. Traber
  • Patent number: 8293101
    Abstract: Apparatus and methods related to centrifugal liquid chromatography are described. An angular velocity can be simultaneously imparted to a large number of chromatographic enclosures. Via centrifugal forces, a mobile phase fluid including a sample can be driven through a stationary phase within the chromatographic enclosure to perform a chromatographic separation process on components of the sample. The use of centrifugation as a driving force can allow significantly smaller stationary phase particles to be employed as compared to high performance liquid chromatography (HPLC). Further, for an equivalent chromatographic separation process, the use of centrifugation can provide much greater separation efficiencies than HPLC.
    Type: Grant
    Filed: March 12, 2010
    Date of Patent: October 23, 2012
    Assignee: Terrasep, LLC
    Inventors: Robert R. Kerr, James D. Whitaker
  • Patent number: 8277651
    Abstract: Apparatus and methods related to centrifugal liquid chromatography are described. An angular velocity can be simultaneously imparted to a large number of chromatographic enclosures. Via centrifugal forces, a mobile phase fluid including a sample can be driven through a stationary phase within the chromatographic enclosure to perform a chromatographic separation process on components of the sample. The use of centrifugation as a driving force can allow significantly smaller stationary phase particles to be employed as compared to high performance liquid chromatography (HPLC). Further, for an equivalent chromatographic separation process, the use of centrifugation can provide much greater separation efficiencies than HPLC.
    Type: Grant
    Filed: March 12, 2010
    Date of Patent: October 2, 2012
    Assignee: Terrasep, LLC
    Inventors: Robert R. Kerr, James D. Whitaker, Thomas M. Kerr
  • Patent number: 8277650
    Abstract: Apparatus and methods related to centrifugal liquid chromatography are described. An angular velocity can be simultaneously imparted to a large number of chromatographic enclosures. Via centrifugal forces, a mobile phase fluid including a sample can be driven through a stationary phase within the chromatographic enclosure to perform a chromatographic separation process on components of the sample. The use of centrifugation as a driving force can allow significantly smaller stationary phase particles to be employed as compared to high performance liquid chromatography (HPLC). Further, for an equivalent chromatographic separation process, the use of centrifugation can provide much greater separation efficiencies than HPLC.
    Type: Grant
    Filed: March 12, 2010
    Date of Patent: October 2, 2012
    Assignee: Terrasep, LLC
    Inventors: Robert R. Kerr, James D. Whitaker
  • Publication number: 20120214974
    Abstract: A spin column device, which contains a rigid porous filter that retains its shape during centrifugation, chromatography methods using the device to isolate a desired substance, e.g., a biological molecule, from other substances in a mixture, and kits containing the device with one or more reagents for use in the method.
    Type: Application
    Filed: May 2, 2012
    Publication date: August 23, 2012
    Applicant: BIOVENTURES, INC.
    Inventor: Elliott P. Dawson
  • Patent number: 8241492
    Abstract: Disclosed herein is a simulated moving bed chromatography device comprises a minimum of one stationary pipe with a plurality of inlet/outlet channels, a rotary device next to the stationary pipes, a stationary radial flow segmented vessel communicates with the rotary device, a fluid moving device and a drive means to rotate the rotary device. The counter movement of the stationary phase and the fluid is simulated by rotating the rotary device in the same direction as the circulating fluid flow, and the fluid flows through the flow distribution segments, the stationary phase segments, the outer fluid transfer segments, holes or by passes of the improved partition plates in the flow distribution segments/compartments and outer fluid transfer segments.
    Type: Grant
    Filed: December 20, 2011
    Date of Patent: August 14, 2012
    Inventor: Leon Yuan
  • Patent number: 8187476
    Abstract: A spin column device, which contains a rigid porous filter that retains its shape during centrifugation, chromatography methods using the device to isolate a desired substance, e.g., a biological molecule, from other substances in a mixture, and kits containing the device with one or more reagents for use in the method.
    Type: Grant
    Filed: October 14, 2011
    Date of Patent: May 29, 2012
    Assignee: BioVentures, Inc.
    Inventor: Elliott P. Dawson
  • Patent number: 8182690
    Abstract: The present invention relates to a centrifugal partitioning chromatography device for separating a liquid having at least two phases, and to a centrifugal partitioning chromatography method implemented by this device. A chromatography device according to the invention comprises at least one flat ring (103) which can be rotated about its axis (X?X) of symmetry and comprises a multitude of cells (110) through which the liquid is intended to flow, each cell being provided with two input/output channels (111 and 112) that are intended to make the liquid flow from one cell to another and which open respectively via two inlet/outlet orifices (111a and 112a) of the cell on two radially internal and external sides (113 and 114) of the latter with respect to the axis of rotation.
    Type: Grant
    Filed: November 6, 2008
    Date of Patent: May 22, 2012
    Assignee: Centre National de la Recherche Scientifique
    Inventors: Alain Paul Olivier Foucault, Jack Legrand, Luc Marchal, Charles Elie Pierre Agaise
  • Publication number: 20120037567
    Abstract: The present invention describes a method for constructing a spiral tube support apparatus used in countercurrent chromatography, improvements to countercurrent chromatography tube support design, and methods of using the improved countercurrent chromatography apparatus. The spiral tube support apparatus may be constructed by a shape forming process such as a three dimensional printing process that in turn uses a laser sintering technique, and can be made out of any easily formed material. Shape changes on both the tube support and the top improve the performance of the tube support and ease of manufacturing. The improved tube support and new method of creation permit use in both micro or macro scale preparations and use in small or large molecule preparations. In particular, specific solvent systems are described that permit purification of proteins.
    Type: Application
    Filed: November 18, 2009
    Publication date: February 16, 2012
    Applicant: CC BIOTECH LLC
    Inventors: Martha Knight, Thomas Finn
  • Publication number: 20120031845
    Abstract: A spin column device, which contains a rigid porous filter that retains its shape during centrifugation, chromatography methods using the device to isolate a desired substance, e.g., a biological molecule, from other substances in a mixture, and kits containing the device with one or more reagents for use in the method.
    Type: Application
    Filed: October 14, 2011
    Publication date: February 9, 2012
    Applicant: BIOVENTURES, INC.
    Inventor: Elliott P. Dawson
  • Patent number: 8092681
    Abstract: The invention relates to a method for separating constituents of a liquid feed by liquid-liquid centrifugal chromatography and to a device for implementing it. Constituents (A, B) having different partition coefficients such that they are respectively carried along at unequal velocities by a light solvent (l) and a heavier solvent (L), the feed is continuously injected at an intermediate point of a separation column (col), successive cycles comprising alternately a heavy solvent injection stage and a light solvent injection stage are carried out respectively at the two ends of the column consisting of the interconnection in series of at least one set of separation cells, and the separated solvents (FA, FB) are collected during each one of said stages at the column ends opposite the solvent injection points.
    Type: Grant
    Filed: July 21, 2008
    Date of Patent: January 10, 2012
    Assignee: Institut Francais du Petrole
    Inventors: François Couillard, Alain Foucault, Daniel Durand
  • Patent number: 8062533
    Abstract: A spin column device, which contains a rigid porous filter that retains its shape during centrifugation, chromatography methods using the device to isolate a desired substance, e.g., a biological molecule, from other substances in a mixture, and kits containing the device with one or more reagents for use in the method.
    Type: Grant
    Filed: July 31, 2009
    Date of Patent: November 22, 2011
    Assignee: BioVentures, Inc.
    Inventor: Elliott P. Dawson
  • Publication number: 20110120951
    Abstract: A chromatographic apparatus may include a hydraulic lifting mechanism axially movable from a lowered position to a raised position such that a column and flange of the apparatus are axially movable with the hydraulic lifting mechanism. In a packing system, a mobile column module may be moved into engagement with a packing station by engaging a flange of the column module with a fixture of the packing station. After packing a column of the column module, the column module may be removed from the packing station with a piston head locked in the column to maintain packing pressure. The packing station may be utilized to unpack the column. A hydraulic lifting mechanism may be operated to lower one or more portions of the column module such that the one or more portions may be separately removed from the packing system.
    Type: Application
    Filed: November 23, 2009
    Publication date: May 26, 2011
    Inventors: Thomas Wesley Hampton, Nigel Green
  • Patent number: 7815799
    Abstract: A continuous countercurrent chromatography system has several rotating chambers arranged around a rotational axis. The chambers are provided for receiving a liquid or liquid mixture to be examined, and the individual chambers are interconnected via liquid carrying connections in such a way as to relay two liquids in countercurrent, wherein one liquid first passes through several chambers and is then returned to the chambers first traversed.
    Type: Grant
    Filed: May 14, 2007
    Date of Patent: October 19, 2010
    Inventor: Nikolaus Stephan Pfeiffer
  • Publication number: 20100252503
    Abstract: The present invention relates to a centrifugal partitioning chromatography device for separating a liquid having at least two phases, and to a centrifugal partitioning chromatography method implemented by this device. A chromatography device according to the invention comprises at least one flat ring (103) which can be rotated about its axis (X?X) of symmetry and comprises a multitude of cells (110) through which the liquid is intended to flow, each cell being provided with two input/output channels (111 and 112) that are intended to make the liquid flow from one cell to another and which open respectively via two inlet/outlet orifices (111a and 112a) of the cell on two radially internal and external sides (113 and 114) of the latter with respect to the axis of rotation.
    Type: Application
    Filed: November 6, 2008
    Publication date: October 7, 2010
    Inventors: Alain Paul Olivier Foucault, Jack Legrand, Luc Marchal, Charles Elie Pierre Agaise
  • Publication number: 20100230353
    Abstract: Apparatus and methods related to centrifugal liquid chromatography are described. An angular velocity can be simultaneously imparted to a large number of chromatographic enclosures. Via centrifugal forces, a mobile phase fluid including a sample can be driven through a stationary phase within the chromatographic enclosure to perform a chromatographic separation process on components of the sample. The use of centrifugation as a driving force can allow significantly smaller stationary phase particles to be employed as compared to high performance liquid chromatography (HPLC). Further, for an equivalent chromatographic separation process, the use of centrifugation can provide much greater separation efficiencies than HPLC.
    Type: Application
    Filed: March 12, 2010
    Publication date: September 16, 2010
    Applicant: TERRASEP, LLC
    Inventors: Robert R. Kerr, James D. Whitaker
  • Publication number: 20100230355
    Abstract: Apparatus and methods related to centrifugal liquid chromatography are described. An angular velocity can be simultaneously imparted to a large number of chromatographic enclosures. Via centrifugal forces, a mobile phase fluid including a sample can be driven through a stationary phase within the chromatographic enclosure to perform a chromatographic separation process on components of the sample. The use of centrifugation as a driving force can allow significantly smaller stationary phase particles to be employed as compared to high performance liquid chromatography (HPLC). Further, for an equivalent chromatographic separation process, the use of centrifugation can provide much greater separation efficiencies than HPLC.
    Type: Application
    Filed: March 12, 2010
    Publication date: September 16, 2010
    Applicant: TERRASEP, LLC
    Inventors: Robert R. Kerr, James D. Whitaker
  • Publication number: 20100230354
    Abstract: Apparatus and methods related to centrifugal liquid chromatography are described. An angular velocity can be simultaneously imparted to a large number of chromatographic enclosures. Via centrifugal forces, a mobile phase fluid including a sample can be driven through a stationary phase within the chromatographic enclosure to perform a chromatographic separation process on components of the sample. The use of centrifugation as a driving force can allow significantly smaller stationary phase particles to be employed as compared to high performance liquid chromatography (HPLC). Further, for an equivalent chromatographic separation process, the use of centrifugation can provide much greater separation efficiencies than HPLC.
    Type: Application
    Filed: March 12, 2010
    Publication date: September 16, 2010
    Applicant: TERRASEP, LLC
    Inventors: Robert R. Kerr, James D. Whitaker, Peter G. Traber
  • Publication number: 20100116744
    Abstract: Disclosed is a rotor-stator homogenization device which is characterized in that a rotor-stator homogenizer is connected to a reaction vessel via a joining system, and/or homogenization surfaces are used, and/or a rotor-stator homogenizer is used that is suitable for transferring material. Also disclosed is a method which utilizes said device and is characterized in that the reproducibility, efficiency, yield, waste disposal, automation of the rotor-stator homogenization, etc. is improved, for example, while the risk of contaminations is reduced.
    Type: Application
    Filed: June 28, 2006
    Publication date: May 13, 2010
    Inventor: Frank Vitzthum
  • Patent number: 7553421
    Abstract: Method for sizing the cells of a centrifugal liquid-liquid chromatography “column” (CPC column) consisting of stacked discs on which the cells connected in cascade (in series) by small channels are engraved. Rotation of the stack creates a high centrifugal acceleration field which makes it possible to keep a liquid phase referred to as stationary phase motionless, whereas a mobile phase circulates along the CPC column. The cells are three-dimensional, with two dimensions (L, l) of the cells oriented in a plane substantially normal to the axis of rotation (?) of the disc and a third dimension (e) oriented in a direction substantially parallel to the axis of rotation, and selected so as to be at least equal to one of the other two dimensions (L, l), thus providing higher efficiency. When the scale of the devices has to be changed, the size of the cells is modified ensuring that, in any case, this third dimension (e) is favored so as to be as great as possible.
    Type: Grant
    Filed: March 17, 2005
    Date of Patent: June 30, 2009
    Assignees: Institut Francais du Petrole, Universite de Nantes
    Inventors: Alain Foucault, Jack Legrand, Luc Marchal, Daniel Durand
  • Publication number: 20090050567
    Abstract: A process for continuous or quasi-continuous purification of a multi-component mixture (F) by means of individual chromatographic columns through which the mixture is fed by means of at least one solvent (s), is proposed.
    Type: Application
    Filed: April 27, 2006
    Publication date: February 26, 2009
    Applicant: Eidgenossische Technische Hochschule Zurich
    Inventors: Lars Aumann, Massimo Morbidelli
  • Publication number: 20090039025
    Abstract: The invention relates to a method for separating constituents of a liquid feed by liquid-liquid centrifugal chromatography and to a device for implementing it. Constituents (A, B) having different partition coefficients such that they are respectively carried along at unequal velocities by a light solvent (l) and a heavier solvent (L), the feed is continuously injected at an intermediate point of a separation column (col), successive cycles comprising alternately a heavy solvent injection stage and a light solvent injection stage are carried out respectively at the two ends of the column consisting of the interconnection in series of at least one set of separation cells, and the separated solvents (FA, FB) are collected during each one of said stages at the column ends opposite the solvent injection points.
    Type: Application
    Filed: July 21, 2008
    Publication date: February 12, 2009
    Inventors: Francois Couillard, Alain Foucault, Daniel Durand
  • Publication number: 20090039024
    Abstract: Embodiments of the present invention feature a device (1) and method for performing steps of a multi-step process in parallel. The device (1) and method feature a rotor assembly (13) having vessel stations (33) and stator assembly (15) having work positions. The rotor assembly (13) rotates the vessels (17) to the work stations to perform steps of a multi-step process at the same time.
    Type: Application
    Filed: June 19, 2006
    Publication date: February 12, 2009
    Applicant: WATERS INVESTMENTS LIMITED
    Inventors: Kevin Jenkins, Paul D. Rainville, Sylvain Cormier
  • Publication number: 20080264865
    Abstract: The present invention is directed to methods and compositions for separating and isolating target molecules. In particular, the present invention comprises devices, such as CCDs, that contain particles without the need for support structures. Chromatography separation techniques, including but not limited to, ion exchange, size separation, affinity chromatography, ion exclusion, ligand exchange, reversed phase and normal phase partitioning, are used in the CCD. Methods also include low, medium and high pressure liquid chromatography. Such methods can be used for analytical, semi-preparative processes, initial clarification, preparative filtration and process scale applications.
    Type: Application
    Filed: March 25, 2008
    Publication date: October 30, 2008
    Inventor: Heath H. Herman
  • Patent number: 7422685
    Abstract: The invention relates to a method for separating constituents of a liquid feed by liquid-liquid centrifugal chromatography and to a device for implementing it. Constituents (A, B) having different partition coefficients such that they are respectively carried along at unequal velocities by a light solvent (1) and a heavier solvent (L), the feed is continuously injected at an intermediate point of a separation column (col), successive cycles comprising alternately a heavy solvent injection stage and a light solvent injection stage are carried out respectively at the two ends of the column consisting of the interconnection in series of at least one set of separation cells, and the separated solvents (FA, FB) are collected during each one of said stages at the column ends opposite the solvent injection points.
    Type: Grant
    Filed: June 28, 2004
    Date of Patent: September 9, 2008
    Assignees: Institut Francais du Petrole
    Inventors: François Couillard, Alain Foucault, Daniel Durand
  • Patent number: 7399898
    Abstract: Disclosed is a simulated moving bed (SMB) adsorptive separation system in which at least one component of a fluid mixture is brought into contact with a solid adsorbent and the adsorbed component is desorbed with a desorbent, wherein the improvement comprises an inert ball layer is formed on the upper part of the solid absorbent filled in the simulated moving bed.
    Type: Grant
    Filed: April 6, 2005
    Date of Patent: July 15, 2008
    Assignee: Samsung Petrochemicals Co., Ltd.
    Inventors: Jin Suk Lee, Nam Cheol Shin
  • Patent number: 7351333
    Abstract: A centrifuge (10) is provided with a cantilevered shaft (18) upon which the main drive rotor (26) is located. A bobbin (62) is attached to the planetary gear shaft (44), which is driven in planetary motion by the rotation of the rotor (26) and its toothed engagement with the shaft (18) of the cantilevered rotor. A flying lead section (72) extends from the bobbin (62) through an aperture in the cantilevered rotor (26). The arrangement provides a centrifuge structure which can handle higher rotational speeds while maintaining resolution. The flying lead section (72) provides a simple path for the inlet and outlet leads (84,86) which reduces dead volume and increases reliability. The bobbin (62) supports at least one coil (68). Rotor component (44) includes at least one bearing (50, 52).
    Type: Grant
    Filed: October 4, 2004
    Date of Patent: April 1, 2008
    Assignee: Brunel University
    Inventors: David W. Hawes, Lee Janaway, Ian A. Sutherland, Philip L. Wood
  • Patent number: 7347943
    Abstract: The present invention is directed to methods and compositions for separating and isolating target molecules. In particular, the present invention comprises devices, such as CCDs, that contain particles without the need for support structures. Chromatography separation techniques, including but not limited to, ion exchange, size separation, affinity chromatography, ion exclusion, ligand exchange, reversed phase and normal phase partitioning, are used in the CCD. Methods also include low, medium and high pressure liquid chromatography. Such methods can be used for analytical, semi-preparative processes, initial clarification, preparative filtration and process scale applications.
    Type: Grant
    Filed: August 29, 2005
    Date of Patent: March 25, 2008
    Assignee: Kinetic Biosystems Inc.
    Inventor: Heath H. Herman
  • Publication number: 20080047900
    Abstract: A filter, comprising: a housing defining an inner cavity; an inner sleeve disposed within the housing, the inner sleeve having portions in a spaced relationship with respect to the an interior surface of the inner cavity; a plurality of tab members extending away from the inner sleeve, the tab members being configured to be removably secured to a feature of a cap, the cap being configured to engage the housing to provide a first position and a second position with respect to the housing, wherein the first position seals the inner sleeve to the housing and a fluid filtration path from an inlet of the filter to an outlet of the filter is defined by the inner sleeve and the second position provides a fluid drain path through an opening in the cap, the fluid drain path being closed when the cap is in the first position; and a filtration media disposed within an interior area of the inner sleeve, wherein the filtration media is disposed in the fluid filtration path and fluid flow through the outlet opening must pa
    Type: Application
    Filed: August 25, 2007
    Publication date: February 28, 2008
    Inventors: Christopher R. Reamsnyder, Craig E. Bishop
  • Patent number: 7118804
    Abstract: A method of using a pellet composed of an aggregate of distinct beads of a chromatography media. The pellet is coherent and capable of being rapidly hydrated on addition of water to form a gel wherein said beads are swollen and substantially uniformly dispersed in the water phase and a combination of the a ligand with the chromatography media.
    Type: Grant
    Filed: July 15, 2003
    Date of Patent: October 10, 2006
    Assignee: Pierce Biotechnology, Inc.
    Inventor: Connie A. Draveling
  • Patent number: 7090774
    Abstract: The invention encompasses a method of packing and unpacking a column chamber. A mixture of a fluid and a matrix material are introduced through a column chamber inlet so that the matrix material is packed within a column chamber to form a packed column. The column chamber having the column chamber inlet or first port for receiving the mixture further has an outlet port and an actuator port. The outlet port is partially closed for capturing the matrix material and permitting the fluid to flow therepast by rotating relative one to the other of a rod placed in the actuator port. Further rotation relative one to the other of the rod and the column chamber opens the outlet and permits the matrix material and the fluid to flow therethrough thereby unpacking the matrix material from the column chamber.
    Type: Grant
    Filed: October 23, 1998
    Date of Patent: August 15, 2006
    Assignee: Battelle Memorial Institute
    Inventors: David A. Holman, Cynthia J. Bruckner-Lea, Fred J. Brockman, Darrell P. Chandler
  • Patent number: 6979402
    Abstract: An apparatus is presented for separating chemicals using adsorption separation methods. The apparatus uses a plurality of adsorption units holding adsorbent, where the adsorption units positioned in a cylindrical spool, are serially connected and the spool is rotated to shift the relative position of the feeds and drawoffs to the apparatus.
    Type: Grant
    Filed: December 19, 2003
    Date of Patent: December 27, 2005
    Assignee: UOP LLC
    Inventors: Robert T. Sprague, Gavin P. Towler, Anil R. Oroskar
  • Patent number: 6942804
    Abstract: The present invention is directed to methods and compositions for separating and isolating target molecules. In particular, the present invention comprises devices, such as CCDs, that contain particles without the need for support structures. Chromatography separation techniques, including but not limited to, ion exchange, size separation, affinity chromatography, ion exclusion, ligand exchange, reversed phase and normal phase partitioning, are used in the CCD. Methods also include low, medium and high pressure liquid chromatography. Such methods can be used for analytical, semi-preparative processes, initial clarification, preparative filtration and process scale applications.
    Type: Grant
    Filed: November 12, 2002
    Date of Patent: September 13, 2005
    Assignee: Kinetic Biosystems, Inc.
    Inventor: Heath H. Herman
  • Patent number: 6913692
    Abstract: The rotating device for a centrifugal partition chromatograph comprises at least one thick-walled cylindrical body (1) that can be driven in rotation around its axis (14), the said cylindrical body (1) comprising several cells with a height less than a determined height. The cells, arranged in the housings (11), have an elongated shape arranged along a direction with a radial component with regard to the rotation axis (14) of the said body (1) and connected to each other in series through ducts. The single piece cylindrical body (1) has a thick wall, with a height (H) at least twice as high as the said determined height, the said cells being arranged at several different heights. For example, several hundred cells may be arranged in the single piece cylindrical body (1). The rotating device resists pressures well above 100 bars and can be used for industrial applications.
    Type: Grant
    Filed: June 20, 2003
    Date of Patent: July 5, 2005
    Assignee: Partus Technologies
    Inventors: Rodolphe Margraff, Pierre Garret, Jean-Hugues Renault
  • Publication number: 20040173534
    Abstract: The rotating device for a centrifugal partition chromatograph comprises at least one thick-walled cylindrical body (1) that can be driven in rotation around its axis (14), the said cylindrical body (1) comprising several cells with a height less than a determined height. The cells, arranged in the housings (11), have an elongated shape arranged along a direction with a radial component with regard to the rotation axis (14) of the said body (1) and connected to each other in series through ducts. The single piece cylindrical body (1) has a thick wall, with a height (H) at least twice as high as the said determined height, the said cells being arranged at several different heights.
    Type: Application
    Filed: June 20, 2003
    Publication date: September 9, 2004
    Inventors: Rodolphe Margraff, Pierre Garret, Jean-Hugues Renault
  • Patent number: 6762057
    Abstract: A sample separation apparatus including a porous, or rough, capillary column. The porous capillary column includes a matrix which defines pores, and may be formed from a material such as porous silicon. Alternatively, the capillary column may have a rough surface of hemispherical grain silicon. The capillary column is defined in a surface of a substrate, such as silicon. The sample separation apparatus may include a stationary phase or a capture substrate disposed on the surfaces thereof. The sample separation apparatus may also include a detector positioned proximate the capillary column. A variation of the sample separation apparatus includes an electrode proximate each end of the capillary column. The sample separation apparatus may be employed to effect various types of chromatographic separation, electrophoretic separation, and analyte identification.
    Type: Grant
    Filed: November 18, 1999
    Date of Patent: July 13, 2004
    Assignee: Micron Technology, Inc.
    Inventor: Terry L. Gilton
  • Publication number: 20040099605
    Abstract: A process for reaction and separation which comprises inputting a process material into at least one column of a plurality of columns wherein each column has at least one inlet for accepting flow from another column or group of columns, an external feed stream, an external eluent stream or a combination thereof, and each column has at least one outlet for connecting to another column, a group of columns, an external product stream or a combination thereof. Each column is independently operable in an up-flow or down-flow mode and connected independently to one of the group comprising another column, an external feed stream, an external eluent stream, an external product stream and combinations thereof.
    Type: Application
    Filed: July 29, 2002
    Publication date: May 27, 2004
    Inventors: Chen-Chou Chiang, David L. Fair
  • Patent number: 6717136
    Abstract: A microfluidic device comprising an MS-analyte presentation unit for a EDI-MS apparatus, said unit comprising an essentially planar support plate which on one side has one, two or more ports (MS-ports) comprising an area (EDI area) for presenting the MS-analyte to a mass spectrometer. The EDI area comprises a layer I of conducting material. The characteristic feature of the device is that layer (I) has a conductive connection and/or that there is a calibrator area in the proximity of the MS-port.
    Type: Grant
    Filed: March 19, 2001
    Date of Patent: April 6, 2004
    Assignee: Gyros AB
    Inventors: Per Andersson, Helene Derand, Magnus Gustafsson, Anders Palm, Sussanne Wallenborg
  • Patent number: 6709743
    Abstract: A pellet composed of an aggregate of distinct beads of a chromatography media. The pellet is coherent and capable of being rapidly hydrated on addition of water to form a gel wherein said beads are swollen and substantially uniformly dispersed in the water phase.
    Type: Grant
    Filed: May 23, 2002
    Date of Patent: March 23, 2004
    Assignee: Pierce Biotechnology, Inc.
    Inventor: Connie A. Draveling
  • Patent number: 6663779
    Abstract: A continuous annular chromatography (CAC) device is disclosed including a stationary column head, a stationary eluate ring, a rotating body which is located between said column head and said collecting ring and which can rotate about a vertical axis and contains a chromatographic separating medium and a drive device. An extension can be coupled to a drive shaft of the drive device with a rapidly detachable connection.
    Type: Grant
    Filed: February 19, 2002
    Date of Patent: December 16, 2003
    Assignee: Prior Separation Technology GmbH
    Inventors: Adalbert Prior, Joachim Prior, Juergen Wolfgang
  • Publication number: 20030173299
    Abstract: A method for fractionating a solution into two or more fractions by a chromatographic simulated moving bed (SMB) process, wherein the separation system comprises at least two separation profiles in the same loop.
    Type: Application
    Filed: March 11, 2003
    Publication date: September 18, 2003
    Inventors: Heikki Heikkila, Goran Hyoky, Jarmo Kuisma, Hannu Paananen
  • Publication number: 20030146161
    Abstract: The present invention is directed to methods and compositions for separating and isolating target molecules. In particular, the present invention comprises devices, such as CCDs, that contain particles without the need for support structures. Chromatography separation techniques, including but not limited to, ion exchange, size separation, affinity chromatography, ion exclusion, ligand exchange, reversed phase and normal phase partitioning, are used in the CCD. Methods also include low, medium and high pressure liquid chromatography. Such methods can be used for analytical, semi-preparative processes, initial clarification, preparative filtration and process scale applications.
    Type: Application
    Filed: November 12, 2002
    Publication date: August 7, 2003
    Inventor: Heath H. Herman
  • Patent number: 6576137
    Abstract: By regulating separation system's relative parameters, this broad and generalized separation process is disclosed to distinguish the fundament between this invention and chromatography for superior cost-effectiveness. A different mass transfer contacting method and differential set-up between two phases are applied on the disclosed apparatus to achieve purposed efficiency. This continuous separation process can be furnished as the integration of multiple partial fluidized beds or cells; operated under designated pressure level to instantaneous and simultaneous proceeding of determined mass-transfer phenomena. All zones within the process are simultaneously proceeded to isolate one product or multiple products from feed mixtures with other proceeding zones of feeding, impurity stripping, regeneration and washing. The differential mass transfer contact method is disclosed for efficient consumption of both solid phase and mobile phase.
    Type: Grant
    Filed: July 3, 2000
    Date of Patent: June 10, 2003
    Inventor: Hsien-Chih Ma
  • Patent number: 6565752
    Abstract: The present invention relates to a new separation method and a device therefor, especially to a device and method for chromatographic chemical analysis. In the method according to the invention, the driving of the mobile phase fluid in, through and out the separation channel, said channel being defined by at least two channel elements, is mainly caused by a relative movement of at least one channel element compared to at least one of the other channel elements preferably the movement of the mobile phase fluid and the sample to be separated through the separation channel is sustained by relief elements, such as one or more protrusions, recesses, holes or irregular porous-like structures, which are arranged on at least one of the channel elements.
    Type: Grant
    Filed: March 2, 2000
    Date of Patent: May 20, 2003
    Assignee: Vrije Universiteit Brussel
    Inventors: Gino Baron, Gert Desmet