Chromatography Patents (Class 210/656)
  • Patent number: 11325104
    Abstract: A system and method of applied radial technology chromatography using a plurality of beads is disclosed, with each bead comprising one or more pores therein having a diameter of about 250 ? to about 5000 ?, and each bead having an average radius between about 100 ?m to about 250 ?m. Also disclosed are processes for selecting beads for use in a radial flow chromatography column, and for purifying an unclarified feed stream using a radial flow chromatography column.
    Type: Grant
    Filed: December 7, 2018
    Date of Patent: May 10, 2022
    Assignee: EMP BIOTECH GMBH
    Inventors: Alistair J. Hurst, Derek W. K. Levison
  • Patent number: 11280770
    Abstract: A separating column for use with a filling tube to prepare for chromatography is disclosed. The separating column includes an axially movable spring stamp; and a plurality of springs coupled to the stamp. Moreover, the stamp is depressed directly into the column tube during column packing via the filling tube. Additionally, the stamp is fixed in the column tube while maintaining the packing pressure so that the springs are compressed during the packing process and press the stamp permanently and dynamically onto a chromatographic bed.
    Type: Grant
    Filed: April 9, 2019
    Date of Patent: March 22, 2022
    Assignee: Dr. Albin Maisch High Performance LC GmbH
    Inventor: Albin Maisch
  • Patent number: 11280769
    Abstract: The disclosure relates to a system for liquid sample introduction into a chromatography system. The system includes a syringe, a first valve in fluid communication with the syringe, a second valve in fluid communication with the sample, a vessel located between, and in fluid communication with, the first and second valves, a third valve in fluid communication with the first valve, the second valve and a chromatography column, and a pump in fluid communication with the third valve and a mobile phase. When the valves are in a first position the syringe draws the sample into the vessel. The mobile phase flows to the chromatography column. When the valves are in a second position, a portion of the mobile phase flows into the vessel, mixing with and pressurizing the sample. When the valves are in a third position, the mixed and pressurized sample flows to the chromatography column.
    Type: Grant
    Filed: November 8, 2019
    Date of Patent: March 22, 2022
    Assignee: Waters Technologies Corporation
    Inventors: Jason Hill, Abhijit Tarafder, Michael O. Fogwill, Thomas McDonald, Sylvain Cormier
  • Patent number: 11266975
    Abstract: Methods of producing and isolating 68Ga, 89Zr, 64Cu, 63Zn, 86Y, 61Cu, 99mTc, 45Ti, 13N, 52Mn, or 44Sc and solution targets for use in the methods are disclosed. The methods of producing 68Ga, 89Zr, 64Cu, 63Zn, 86Y, 61Cu, 99mTc, 45Ti, 13N, 52Mn, or 44Sc include irradiating a closed target system with a proton beam. The system can include a solution target. The methods of producing isolated 68Ga, 89Zr, 64Cu, 63Zn, 86Y, 61Cu, 99mTc, 45Ti, 52Mn, or 44Sc further include isolating 68Ga, 89Zr, 64Cu, 63Zn, 86Y, 61Cu, 99mTc, 45Ti, 52Mn, or 44Sc by ion exchange chromatography. An example target includes a target body including a target cavity for receiving the target material; a housing defining a passageway for directing a particle beam at the target cavity; a target window for covering an opening of the target cavity; and a coolant gas flow path disposed in the passageway upstream of the target window.
    Type: Grant
    Filed: September 9, 2019
    Date of Patent: March 8, 2022
    Assignees: Mayo Foundation for Medical Education and Research, The Brigham and Women's Hospital, Inc.
    Inventors: Timothy R. DeGrado, Mukesh K. Pandey, John Byrne
  • Patent number: 11253795
    Abstract: Chromatography apparatus and methods are described, especially for expanded bed adsorption. A column tube has a process fluid input device at the bottom and a movable piston in the top. The piston is enclosed in the column by a cover plate. The piston body has an inflatable seal, and is connected by a frame to a contact ring which carries another inflatable member to contact the tube wall. Process fluid leaves the operating volume through an opening of the piston and flexible hose, through the enclosed space and out through the cover plate. The space above the piston can be pressurised to control piston movement. The contact ring maintains piston alignment. The inflatable seals are used to fix the piston in position, allow it to slide or allow washing. The piston outlet may include a vortex-inhibitor. Bed and piston levels may be monitored by ultrasound sensors.
    Type: Grant
    Filed: June 14, 2019
    Date of Patent: February 22, 2022
    Assignee: Biotechflow Ltd
    Inventor: Martin John Hofmann
  • Patent number: 11246823
    Abstract: The invention relates to the use of molecularly imprinted polymer(s) or MIPs, of odorous molecule(s), as deodorant agent in particular for selectively trapping molecules that are the cause of human body odour. More particularly by using MIPs which may be obtained via polymerization of “sol-gel” type; it being understood that the polymerization is performed in the presence v) of one or more “templates” of target molecule(s) responsible for human body odour. Another subject of the invention concerns a NI process for preparing MIPs as defined previously, MIPs obtained via this process, and a cosmetic composition comprising at least one MIP as defined previously. Unexpectedly, it appears that the MIPs make it possible to specifically trap precursors of odorous molecules and odorous molecules that may be used in cosmetic formulations, especially those that are the cause of the unpleasant odour of sweat.
    Type: Grant
    Filed: December 16, 2013
    Date of Patent: February 15, 2022
    Assignee: L'OREAL
    Inventors: Andrew Greaves, Franco Manfre, Karsten Haupt
  • Patent number: 11224837
    Abstract: A method is provided in which a carbon dioxide containing flue gas is provided by combusting a carbonaceous fuel in a high pressure steam generating unit using combustion air, and in which the carbon dioxide in the flue gas is at least partially captured and compressed in a carbon dioxide capture and compression unit having a carbon dioxide scrubber operated with an absorbing liquid which is regenerated using low pressure steam. The combustion air used in the high pressure steam generating unit is at least partially heated using sensible heat of the flue gas and/or the steam used for regenerating the absorption liquid of the carbon dioxide scrubber is at least partially generated using sensible heat of the flue gas. A corresponding system is also described herein.
    Type: Grant
    Filed: November 12, 2019
    Date of Patent: January 18, 2022
    Assignee: Linde Aktiengesellschaft
    Inventors: Stevan Jovanovic, Ramachandran Krish Krishnamurthy
  • Patent number: 11187683
    Abstract: A high performance liquid chromatography method used for dexrazoxane-related substances is provided, and in the method, a low-density bonding reversed-phase C18 chromatographic column resistant to pure water is employed; a gradient elution is carried out with mobile phase A and mobile phase B as eluents, the mobile phase A being a buffer, and the mobile phase B being an organic solvent; the volume percent of mobile phase A in eluents in a first stage of the gradient elution is not lower than 90%, and the duration of the first stage of the gradient elution ranges from 15˜30 minutes. By means of the analytical method, dexrazoxane is effectively separated from main impurities, and the qualities of the active pharmaceutical ingredients of dexrazoxane and the preparations thereof could be better controlled.
    Type: Grant
    Filed: November 30, 2017
    Date of Patent: November 30, 2021
    Assignee: JIANGSU AOSAIKANG PHARMACEUTICAL CO., LTD.
    Inventors: Xiangfeng Chen, Hongyu Chen, Shuaihua Tian, Min Sun, Changjun Fan
  • Patent number: 11179691
    Abstract: A chemical processing column that includes a column base and an assembly for maintaining plug flow in the column base. The column base is adapted to contain a bed of media. The assembly includes a sleeve removably coupled to the column base, a plate coupled to the plate, and a piston at least partially disposed within the sleeve. The assembly is dynamically movable relative to the column base to adjust a position of the piston relative to the column base to compensate for a change in volume occupied by the bed of media.
    Type: Grant
    Filed: August 12, 2020
    Date of Patent: November 23, 2021
    Assignee: Asahi Kasei Bioprocess America, Inc.
    Inventor: Michael D. Brandt
  • Patent number: 11174175
    Abstract: A sustainable system for removing and concentrating per- and polyfluoroalkyl substances (PFAS) from water. The system includes an anion exchange vessel having a selected anion exchange resin therein configured to remove PFAS from the water. A line coupled to the vessel introduces a flow of water contaminated with PFAS such that the PFAS bind to the selected anion exchange resin and are thereby removed from the water. A regenerant solution line is coupled to the anion exchange vessel to introduce an optimized regenerant solution to the anion exchange vessel to remove the PFAS from the anion exchange resin, thereby regenerating the anion exchange resin and generating a spent regenerant solution comprised of the removed PFAS and the optimized regenerant solution. A separation and recovery subsystem recovers the optimized regenerant solution for reuse and separates and concentrates the removed PFAS.
    Type: Grant
    Filed: April 29, 2020
    Date of Patent: November 16, 2021
    Assignee: Emerging Compounds Treatment Technologies, Inc.
    Inventors: Michael G. Nickelsen, Steven E. Woodard, John C. Berry
  • Patent number: 11167993
    Abstract: The present invention is to provide a surface-modified nanodiamond having excellent affinity for an organic solvent and a resin and having high dispersibility in an organic solvent and in a resin. The surface-modified nanodiamond according to an embodiment of the present invention has a structure in which a surface of a nanodiamond particle is modified by a group represented by Formula (1) below. In Formula (1), R1 represents an aliphatic hydrocarbon group having 6 or more carbons. R2 and R3 may be the same or different and are each a hydrogen atom, an aliphatic hydrocarbon group having from 1 to 3 carbons, or a group represented by Formula (2) below.
    Type: Grant
    Filed: June 6, 2018
    Date of Patent: November 9, 2021
    Assignee: DAICEL CORPORATION
    Inventor: Kouichi Umemoto
  • Patent number: 11155678
    Abstract: A method for chromatographically separating a mixture containing a perfluoro(poly)ether group-containing monoalcohol compound represented by the following formula (2) and a perfluoro(poly)ether group-containing dialcohol compound represented by the following formula (3): A-Pf-Z??(2) Z-Pf-Z??(3) wherein Pf represents a divalent perfluoropolyether group and A and Z are as defined herein, the method including: adsorbing the mixture onto a stationary phase, and eluting the perfluoro(poly)ether group-containing monoalcohol compound represented by formula (2) by one mobile phase selected from hydrofluorocarbons, hydrochlorofluorocarbons, hydrofluoroethers, fluorine-containing ester solvents, and fluorine-containing aromatic solvents to separate the perfluoro(poly)ether group-containing monoalcohol compound represented by formula (2) from the perfluoro(poly)ether group-containing dialcohol compound represented by formula (3).
    Type: Grant
    Filed: August 24, 2017
    Date of Patent: October 26, 2021
    Assignee: DAIKIN INDUSTRIES, LTD.
    Inventors: Masato Naitou, Takashi Nomura, Shinya Takano
  • Patent number: 11150224
    Abstract: The present disclosure relates to methodologies, systems, apparatus, and kits for introducing a sample within a chromatography system. A makeup pump is configured to pump a makeup fluid through a first restrictor into the chromatography system upstream of the column and downstream of a mobile phase pump. The first restrictor is located upstream of a column and downstream of makeup pump and a sample fluid pump. Decreasing an output volume of the makeup pump can direct a sample fluid from the sample fluid pump through the first restrictor to the column. Increasing an output volume of the makeup pump can direct the sample fluid to a second restrictor located downstream of the makeup pump and in parallel with the column and the detector.
    Type: Grant
    Filed: May 29, 2019
    Date of Patent: October 19, 2021
    Assignee: Waters Technologies Corporation
    Inventors: Michael O. Fogwill, Joseph D. Michienzi
  • Patent number: 11101121
    Abstract: For increasing a speed of fragmentation of a sample, such as a protein and a peptide, to enhance introduction efficiency into a detector, such as a mass spectrometer, a liquid feeding pump 2, a sample injector 3, and a separation column 6 that are connected via pipes are included, and further, a heating unit 4 for heating a pipe between the sample injector 3 and the separation column 6, and a pressure regulating unit 5 provided between the heating unit 4 and the separation column 6 for regulating the inner pressure of the pipe heated by the heating unit 4, are included.
    Type: Grant
    Filed: March 30, 2016
    Date of Patent: August 24, 2021
    Assignee: HITACHI HIGH-TECH CORPORATION
    Inventors: Hideki Hasegawa, Shun Kumano, Yuichiro Hashimoto
  • Patent number: 11061003
    Abstract: Described are a method and apparatus for diluting a chromatographic sample. The method includes repeating an alternating acquisition of sample fluidic plugs each having an incremental volume of sample and diluent fluidic plugs each having an incremental volume of diluent to obtain a stack of alternating sample and diluent fluidic plugs. The stack is inserted into a flow of a mobile phase in a chromatography system. Alternatively, the method includes repeating the steps of injecting an incremental volume of sample into a chromatographic system flow and providing an incremental volume of mobile phase into the chromatographic system flow. In either implementation, the dilution ratio of the sample equals the sum of the incremental volumes of the sample and the diluent or mobile phase divided by the sum of the incremental volumes of the sample.
    Type: Grant
    Filed: October 15, 2019
    Date of Patent: July 13, 2021
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Martin Gilar, Keith Fadgen, Peyton C. Beals, Sylvain Gilles Cormier, Richard Wayne Andrews, Geoff C. Gerhardt, Jordan Kornfeld, Thomas S. McDonald
  • Patent number: 11059856
    Abstract: Method for recovering a target protein from a feedstock comprising said target protein and at least one impurity compound selected from host cell proteins (HCP), DNA, RNA or other nucleic acid, the target protein being characterized by a hydrodynamic radius Rh1 and the impurity compound being characterized by a hydrodynamic radius Rh2, wherein Rh1>Rh2, comprising the following steps (i) to (iv) and optionally step (v): (i) providing a polymeric mesh comprising at least one crosslinked polymer containing positively charged amino groups, wherein the polymer has a pore size exclusion limit Rhi which can be set variably; (ii) adapting the variable pore size exclusion limit Rhi of the polymeric mesh such that Rh2<Rhi and Rh1>Rhi; (iii) contacting the polymeric mesh with the feedstock; (iv) separating the polymeric mesh containing the retained impurity compound from the feedstock containing the excluded target protein.
    Type: Grant
    Filed: September 15, 2017
    Date of Patent: July 13, 2021
    Assignee: Klawego GmbH & Co. KG
    Inventors: Klaus Gottschall, Lothar Britsch, Evelyn Gottschall
  • Patent number: 11047834
    Abstract: A preparative liquid chromatograph that separates components in a sample in a separating column and captures a plurality of target components in an eluate from the separating column in individual trap columns, includes: a column switching means configured to switch passages to cause the eluate having been eluted from the separating column and passed through a detector to selectively flow into one of the trap columns, a passage switching means disposed in a passage between the detector and the column switching means and configured to switch between a first state in which the eluate flows to the column switching means and a second state in which the eluate is discharged without flowing to the column switching means. For the switching among the trap columns, the passage switching means and the column switching means are controlled to set the passage switching means in the second state (step S17) before performing the switching operation of the column switching means (step S18).
    Type: Grant
    Filed: July 6, 2016
    Date of Patent: June 29, 2021
    Assignee: Shimadzu Corporation
    Inventor: Tomoyuki Yamazaki
  • Patent number: 11029298
    Abstract: A gas analyzer includes a sample inlet, a sample outlet, a detector, a monitoring component, and a controller. The sample inlet is configured to receive a sample and is coupled to the sample outlet. The detector is operably disposed between the sample inlet and the sample outlet and is configured to provide an indication relative to the sample. The monitoring component is configured to provide a diagnostic indication regarding at least one component of the gas analyzer. The controller is configured to control flow through the gas analyzer and is operably coupled to the detector to analyze the sample, provide the analysis to the monitoring component, and provide the indication of health to an output.
    Type: Grant
    Filed: March 2, 2017
    Date of Patent: June 8, 2021
    Assignee: Rosemount Inc.
    Inventors: Suresh S. Satpute, Sayali P. Gajare, Swati S. Patil
  • Patent number: 11014129
    Abstract: The present invention provides methods for cleaning a Protein A chromatography column employing a media comprising a Protein A ligand derived from the C domain of Staphylococcus aureus, such that the column can be cleaned using both acidic and alkaline solutions.
    Type: Grant
    Filed: June 19, 2014
    Date of Patent: May 25, 2021
    Assignee: EMD Millipore Corporation
    Inventors: Nanying Bian, Sapna Mehtani, John Dana Hubbard
  • Patent number: 10996172
    Abstract: Surface-functionalized nano structures, arrays of the nanostructures, and method for using the arrays in surfaced-enhanced spectroscopy and dielectric sensing applications, such as surface-enhanced infrared absorption spectroscopy, are provided. The nanostructures are functionalized with specific binding moieties that are bound to the nanostructures via phosphonic acid linkers.
    Type: Grant
    Filed: April 27, 2018
    Date of Patent: May 4, 2021
    Assignees: NATIONAL INSTITUTE OF MATERIAL SCIENCE, Northwestern University
    Inventors: Robert P. H. Chang, Shiqiang Li, Peijun Guo, Kai Chen, Keiko Okano, Tadaaki Nagao
  • Patent number: 10988527
    Abstract: The present invention relates to a method for preparing a TNFR-Fc fusion protein mixture comprising a target content of hydrophobic chromatogram peak 3, and to a method for adjusting a content of hydrophobic chromatogram peak 3. Specifically, the present invention relates to (a) a method for preparing a TNFR-Fc fusion protein mixture using a hydrophobic interaction chromatograph medium containing an aromatic functional group, which is pre-equilibrated with an equilibration buffer comprising sodium chloride or ammonium sulfate, and a sample comprising a TNFR-Fc fusion protein mixture liquid produced from mammalian cells, and to a method for adjusting the content of hydrophobic chromatogram peak 3 by hydrophobic chromatography using an equilibration buffer containing a predetermined concentration of sodium chloride or ammonium sulfate.
    Type: Grant
    Filed: December 29, 2015
    Date of Patent: April 27, 2021
    Assignee: LG CHEM, LTD.
    Inventors: Sang Woo Park, Hwa Young Lee, Soon Woong Choi, Chul Ho Jung
  • Patent number: 10968443
    Abstract: The disclosure provides methods for isolating nucleic acids from a biological fluid. In one aspect, the disclosure provides a method for isolating RNA. In another aspect, the disclosure provides a method for isolating DNA. In one aspect, the methods described herein utilize a protocol that combines a detergent-based initial denaturation, protease digestion, and organic extraction followed by column purification that maximizes RNA/DNA yield and preserves RNA/DNA integrity. In yet another aspect, the disclosure provides a kit for isolating RNA and/or DNA.
    Type: Grant
    Filed: December 31, 2015
    Date of Patent: April 6, 2021
    Assignee: THE ROCKEFELLER UNIVERSITY
    Inventors: Klaas Max, Karl Bertram, Kemal Akat, Thomas H. Tuschl, Jenny Li, Kimberly Bogardus
  • Patent number: 10955391
    Abstract: A chromatography system has an associated system volume and a sample dispersion volume. The chromatography system comprises a pump pumping a flow of gradient, a sample manager for introducing a sample into the flow of gradient, and a valve manager fluidically coupled to the pump and to the sample manager. The valve manager includes at least one valve. A first valve of the at least one valve has a plurality of ports including an inlet port that receives the flow of gradient from the pump and an outlet port through which the flow of gradient exits the first valve. The first valve has at least two different, automatically selectable positions. A first position of the at least two different automatically selectable positions operating to change one of the system and sample dispersion volumes of the chromatography system when the first valve is automatically switched into the first position.
    Type: Grant
    Filed: February 17, 2016
    Date of Patent: March 23, 2021
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Sylvain Cormier, Richard W. Andrews, Peyton C. Beals
  • Patent number: 10940401
    Abstract: The present invention provides methods for cleaning or regenerating a chromatography materiel for reuse. The methods of the invention can be used for cleaning or regenerating chromatography columns for reuse in the large-scale manufacture of multiple polypeptide products.
    Type: Grant
    Filed: March 13, 2018
    Date of Patent: March 9, 2021
    Assignee: GENENTECH, INC.
    Inventors: Ekta Mahajan, Joanna So, Jay Werber
  • Patent number: 10933349
    Abstract: An arrangement for mounting components in a heating chamber for heating a fluid of a fluid separation apparatus, wherein the arrangement comprises a mounting board having at least one mounting recess each configured for accommodating at least one component, and the at least one component each configured to be mountable in and/or on the at least one mounting recess.
    Type: Grant
    Filed: January 13, 2015
    Date of Patent: March 2, 2021
    Assignee: Agilent Technologies, Inc.
    Inventors: Ansgar Waldbaur, Armin Steinke, Ralf Schaeffer
  • Patent number: 10928367
    Abstract: A peak extraction method for extracting a true peak from a measured waveform, including acquiring a second derivative waveform; extracting a provisional peak on the basis of a maximum value and/or a minimum value of the second derivative waveform; determining the peak width of the provisional peak on the basis of a model peak function; computing, on the basis of the model peak function, a theoretical value for the height of the provisional peak using two points corresponding to the two ends of the peak width; computing, based on the second derivative waveform, an index value for a variation in the noise on the measured waveform; and computing an S/N ratio, which is a ratio of the peak height theoretical value and the index value, and extracting the provisional peak that is equal to or greater than a preset value as the true peak.
    Type: Grant
    Filed: February 3, 2016
    Date of Patent: February 23, 2021
    Assignee: SHIMADZU CORPORATION
    Inventor: Hiroaki Kozawa
  • Patent number: 10907014
    Abstract: Compositions including one or more polymers are provided. Exemplary polymers include polymeric carbon monoxides. The compositions can be prepared by subjecting a source material to x-rays, optionally at increased pressures. The compositions can be used in a variety of applications, such as fuels, optics, and electronics.
    Type: Grant
    Filed: August 15, 2016
    Date of Patent: February 2, 2021
    Assignee: THE BOARD OF REGENTS OF THE NEVADA SYSTEM OF HIGHER EDUCATION ON BEHALF OF THE UNIVERSITY OF NEVADA, LAS VEGAS
    Inventor: Michael Pravica
  • Patent number: 10900892
    Abstract: There is provided a method of validating a calibration of a sensor represented by a calibrated relationship between concentration of an analyte in a sample and measurements from a sensor of an optical property of a sensing substance, wherein the optical property of the sensing substance has a spectrum that varies with the concentration of the analyte in the sample, and the spectrum has an isosbestic wavelength at which the optical property does not vary with concentration of the analyte, the method comprising making measurements of the optical property at three or more wavelengths of light while the sensing substance is exposed to the sample, determining whether the measurements of the optical property are inconsistent with the calibrated relationship, and outputting a warning signal in response to the measurements of the optical property being inconsistent with the calibrated relationship.
    Type: Grant
    Filed: February 17, 2020
    Date of Patent: January 26, 2021
    Assignee: SciLogica Corp.
    Inventors: Alasdair Allan Mackenzie, Barry Colin Crane, Nicholas Paul Barwell, Praveen Sagar, Robert Perkins
  • Patent number: 10759681
    Abstract: The present disclosure relates to water purification compositions and the method of producing the same. Specifically, the present disclosure relates to compositions containing a substrate that has hydroxyl, thiol, carboxyl or amino groups, a metal oxide, and a carboxylic acid compound, and the method of producing the same. The composition is useful for the removal of soluble phosphorus, selenium, fluoride, arsenic and other heavy metal contaminants that may be present in water. The composition could be of use in numerous fields, including but not limited to, oil and gas, mining, storm water, agricultural runoffs, municipal wastewater, and industrial wastewater.
    Type: Grant
    Filed: June 28, 2018
    Date of Patent: September 1, 2020
    Assignee: BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS
    Inventor: Tito Viswanathan
  • Patent number: 10761072
    Abstract: A temperature gradient chromatography, and apparatus for the same, said method comprising the following: a) dissolving a composition comprising at least one polymer in at least one solvent, to form a polymer solution; b) injecting at least a portion of the polymer solution onto a support material and wherein the support material has a CI from 0.70 to 1.50; c) cooling the support material at a rate greater than, or equal to, 0.2° C./min; d) increasing the temperature of the support material to elute at least some of the polymer; e) generating the chromatogram.
    Type: Grant
    Filed: August 24, 2016
    Date of Patent: September 1, 2020
    Assignee: DOW Global Technologies LLC
    Inventors: Rongjuan Cong, Albert Parrott, Cherry Hollis, Charles Michael Cheatham
  • Patent number: 10753680
    Abstract: A method for separating carbon dioxide from flue gas to generate a high purity CO2 stream.
    Type: Grant
    Filed: March 23, 2018
    Date of Patent: August 25, 2020
    Assignee: GENERAL ELECTRIC TECHNOLOGY GMBH
    Inventors: Andrea Caruso, Olaf Stallmann, Markus Jorg Weitzel, Christian Karl Britz
  • Patent number: 10753915
    Abstract: A method for analyzing and quantifying a panel of drugs in a clinical sample comprises: trapping a first portion of drug parent compounds and their metabolites on a first chromatographic column; trapping a second portion of the drug parent compounds and their metabolites on a second chromatographic column; separately eluting the first and second portions of the drug parent compounds and their metabolites from the first and second chromatographic columns; detecting concentrations of each of the drug parent compounds and metabolites eluted from each of the first and second chromatographic columns with a detector; and summing the detected concentration of each drug parent compound together with the detected concentrations of all of its respective analytes so as to derive a respective total concentration of each drug in the sample.
    Type: Grant
    Filed: July 27, 2016
    Date of Patent: August 25, 2020
    Assignee: Thermo Finnigan LLC
    Inventors: Joseph Herman, Sarah J. Fair, Dayana Argoti
  • Patent number: 10732153
    Abstract: A preparative purification apparatus capable of adjusting a mixing ratio of a mobile phase and an elution solvent contained in a solution to be collected. A liquid feeding unit which feeds an elution solvent to an inlet of a trap column; a flow path switching unit which selectively connects an outlet of the trap column to one of a waste liquid flow path or a recovery flow path; a liquid feed amount measurement unit; and a flow path control unit which performs a control such that the flow path switching unit is connected to the recovery flow path when the amount of the elution solvent fed to the trap column reaches a predetermined initial waste liquid amount, and thereafter, the flow path switching unit is connected to the waste liquid flow path at a timing of reaching a predetermined solution recovery amount.
    Type: Grant
    Filed: August 24, 2015
    Date of Patent: August 4, 2020
    Assignee: Shimadzu Corporation
    Inventors: Tomoyuki Yamazaki, Yosuke Iwata
  • Patent number: 10697944
    Abstract: A portable system 1 for analyzing gaseous flows that vary over time is described, the system comprising a sampling chamber 18, a gas sampling module 7, an ion filtering module 8 and an ion detecting module 9. The gas sampling module 7 is configured to adjust an input gaseous flow Fi of gas particles from the sampling chamber 18, ionize said gas particles and to emit the produced ions, so as to generate an ion flow I. The ion filtering module 8 is configured to controllably select at least one type of ion present in the ion flow I and to generate a corresponding at least one homogeneous ion beam I?, having an intensity representative of the concentration of the corresponding gas particle in the gaseous composition to be analyzed. The ion detecting module 9 is configured to measure the intensity of the at least one ion beam I?.
    Type: Grant
    Filed: July 2, 2015
    Date of Patent: June 30, 2020
    Assignee: Nanotech Analysis S.R.L.
    Inventors: Gianpiero Mensa, Raffaele Correale
  • Patent number: 10684263
    Abstract: A device including a chromatogram display section; a peak-specifying point detector automatically detecting a peak-beginning point on a displayed chromatogram; a peak-specifying point indicator allowing an operator to indicate, as the point in time of a peak-ending point, a point in time on a time axis of an orthogonal coordinate system; a peak-specifying-point candidate designator designating a point at which a straight line extending parallel to the intensity axis and passing through the point in time of the peak-ending point indicated by the peak-specifying point indicator intersects with the chromatogram and a point at which a straight line extending parallel to the time axis and passing through the peak-beginning point detected by the peak-specifying point detector intersects with the straight line extending parallel to the intensity axis and passing through the point in time of the peak-ending point; and a peak-specifying point selector allowing an operator to select either point.
    Type: Grant
    Filed: May 19, 2015
    Date of Patent: June 16, 2020
    Assignee: SHIMADZU CORPORATION
    Inventor: Momoyo Yamakawa
  • Patent number: 10684286
    Abstract: The invention provides a method for analyzing a protamine sample for the presence or amount of at least one nucleotidic impurity. The invention also provides a method for the quantitation of peptides in a sample of protamine comprising four major peptides and at least one related impurity.
    Type: Grant
    Filed: April 6, 2017
    Date of Patent: June 16, 2020
    Assignee: Fresenius Kabi USA, LLC
    Inventors: Pearle Torralba, David Jeschke, Kamlesh Patel
  • Patent number: 10669307
    Abstract: The present invention relates to a device for separating and/or isolating substances in or from a mixture with improved utilization of the capacity of chromatographic media, the device comprising a first chromatography system, a second chromatography matrix downstream of the first chromatography system, and a sensor for detecting the substances present in the fluid. Furthermore, the present invention relates to both the use of said device and a method for separating and/or isolating substances in or from a mixture in a fluid.
    Type: Grant
    Filed: May 12, 2015
    Date of Patent: June 2, 2020
    Assignee: Sartorius Stedim Biotech GmbH
    Inventors: Louis Villain, Jörg Mittelstät, Ana Raquel Santos De Matos Fortuna, Katrin Töppner
  • Patent number: 10661250
    Abstract: In embodiments, a packing material for supported liquid extraction has a sorbent media that includes synthetic silica particles. In embodiments, the synthetic silica particles can have physical properties relating to one or more of particle surface area, shape, size, or porosity. In one embodiment, synthetic silica particles have a surface area less than about 30 m2/g. In another embodiment, the synthetic silica particles have an approximately uniform particle shape. In further examples, synthetic silica particles have a particle size in a range of about 30-150 ?m inclusive or greater than about 200 ?m. In another embodiment, synthetic silica particles are arranged to have a pore size greater than about 500 Angstroms. In an embodiment, an apparatus for supported liquid extraction includes a container and a sorbent media that includes synthetic silica particles. In a further embodiment, a method for extracting target analytes through supported liquid extraction is provided.
    Type: Grant
    Filed: December 19, 2018
    Date of Patent: May 26, 2020
    Assignee: AGILENT TECHNOLOGIES, INC.
    Inventors: Kunqiang Jiang, Derick Lucas, Bruce Richter
  • Patent number: 10632461
    Abstract: According to one embodiment, a sealed chemical container has a bonding area where a flexible sheet-like member is firmly bonded onto a plate, and the bonding area defines a first compartment which can be swollen and an outflow passage which communicates with the first compartment via an opening. A first seal portion is weakly bonded onto the plate compared to the bonding area to define the first compartment to block communication between the first compartment and an outflow passage. The first seal portion is peeled off by an increase in internal pressure in the first compartment to allow communication between the first compartment and the outflow passage.
    Type: Grant
    Filed: September 28, 2017
    Date of Patent: April 28, 2020
    Assignee: Canon Medical Systems Corporation
    Inventors: Hiroaki Kushima, Nobuo Kawamura, Tetsuya Kuwabara, Sotaro Taki
  • Patent number: 10627289
    Abstract: An improved method for integrating curve peaks as compared to techniques such as the trapezoidal rule wherein integration parameters are at fixed x-axis positions. Integration parameters are instead specified relative to a peak center, which allows the peak to shift over time due to hardware changes, temperature fluctuation, pressure changes, etc., while maintaining integration parameters at optimal locations for that peak. As such, the present disclosure finds particular utility in spectroscopy wherein, in the case of Raman spectroscopy, for example, specific wavenumber shift locations may drift over time, leading to inaccurate results based upon absolute integration parameters.
    Type: Grant
    Filed: October 19, 2018
    Date of Patent: April 21, 2020
    Assignee: Kaiser Optical Systems Inc.
    Inventor: Patrick Wiegand
  • Patent number: 10618951
    Abstract: The present invention relates to cell culture media containing combinations of proteins, as well as methods of making the cell culture media, and methods of using the cell culture media to improve growth characteristics of cultured cells.
    Type: Grant
    Filed: June 21, 2016
    Date of Patent: April 14, 2020
    Assignee: Ventria Biosciences Inc.
    Inventors: Steven Clyde Pettit, Mary Ann Michelle Fernandez Santos, Ning Huang
  • Patent number: 10604788
    Abstract: Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
    Type: Grant
    Filed: August 29, 2019
    Date of Patent: March 31, 2020
    Assignee: HANDYLAB, INC.
    Inventors: Betty Wu, John S. Althaus, Nikhil Phadke, Sundaresh N. Brahmasandra, Kalyan Handique, Aaron Kehrer, Gene Parunak, Cecelia Haley, Ted Springer
  • Patent number: 10605671
    Abstract: To provide a chromatograph data processing device and the like that can estimate and calculate a retention index or a retention time that would be measured by performing programmed-temperature analysis with any desired rate of temperature increase from known retention indices measured by programmed-temperature analysis, a receiving unit that receives a rate of temperature increase, an RI-r relationship storage unit that stores an RI-r relationship, the RI-r relationship being a relationship between a retention index and a rate of temperature increase and being identified based on a plurality of retention indices with respect to different rates of temperature increase and the corresponding rates of temperature increase, and a retention index calculation unit that calculates an estimated retention index based on the RI-r relationship, the estimated retention index being a retention index corresponding to the received rate of temperature increase received by the receiving unit.
    Type: Grant
    Filed: December 9, 2014
    Date of Patent: March 31, 2020
    Assignee: HORIBA STEC, CO., LTD.
    Inventors: Tsuneaki Maeda, Nanako Sasaki
  • Patent number: 10596254
    Abstract: Highly effective vaccine compositions are constructed according to the methods of this invention. The methods are amenable to use with any peptidic antigen sequence and involve covalent attachment of an immunostimulatory nucleotide sequence to an antigenic peptide sequence. Preferred antigenic peptides are fusion peptides made up of one or more CTL epitope peptides in sequence fused to a T helper peptide.
    Type: Grant
    Filed: April 26, 2018
    Date of Patent: March 24, 2020
    Assignee: City of Hope
    Inventor: Don J. Diamond
  • Patent number: 10585076
    Abstract: A gas-liquid separator according to an embodiment of the present invention separates a mobile phase containing a gas and a liquid into a gas and a liquid. The gas-liquid separator according to the embodiment of the present invention includes an introduction flow channel to which a mobile phase is introduced, and a plurality of discharge flow channels connected to the introduction flow channel. A gas and a liquid are discharged from a discharge port of the discharge flow channel.
    Type: Grant
    Filed: September 17, 2014
    Date of Patent: March 10, 2020
    Assignee: SHIMADZU CORPORATION
    Inventors: Hiroomi Goto, Risa Kajiyama, Takahiro Mori
  • Patent number: 10525376
    Abstract: The present invention is directed to affinity chromatography devices that separate a targeted protein or antibody from an aqueous mixture containing the targeted protein or antibody. The chromatography device may contain a stacked membrane assembly or a wound membrane assembly. The membrane assemblies include at least one polymer membrane that contains therein inorganic particles. The polymer membrane and/or the inorganic particles have an affinity ligand bonded thereto. The affinity ligand may be a protein, an antibody, or a polysaccharide that reversibly binds to the targeted protein or antibody. The chromatography device may be repeatedly used and may be cleaned with a caustic solution between uses. The chromatography devices may have a dynamic binding capacity (DBC) of at least 30 mg/ml (or 0.07 micromol/ml) at 10% breakthrough at a residence time of 20 seconds or less.
    Type: Grant
    Filed: April 8, 2016
    Date of Patent: January 7, 2020
    Assignee: W. L. Gore & Associates, Inc.
    Inventor: Michael C. McManaway
  • Patent number: 10526367
    Abstract: The present invention is directed to affinity chromatography devices that separate a targeted protein or antibody from an aqueous mixture containing the targeted protein or antibody. The chromatography device may contain a stacked membrane assembly or a wound membrane assembly. The membrane assemblies include (1) at least one polymer membrane that contains therein inorganic particles and (2) at least one impermeable layer (e.g., a thermoplastic polymer in a solid state). The polymer membrane and/or the inorganic particles have an affinity ligand bonded thereto. The affinity ligand may be a protein, an antibody, or a polysaccharide that reversibly binds to the targeted protein or antibody. The chromatography device may be repeatedly used and may be cleaned with a caustic solution between uses. The chromatography devices has a dynamic binding capacity (DBC) of at least 30 mg/ml (or 0.07 micromol/ml) at 10% breakthrough at a residence time of 20 seconds or less.
    Type: Grant
    Filed: January 19, 2017
    Date of Patent: January 7, 2020
    Assignee: W. L. Gore & Associates, Inc.
    Inventors: Michael C. McManaway, Brian J. Swetlin, Kenneth S. Zukor
  • Patent number: 10520479
    Abstract: In a liquid chromatograph mass spectrometer (LC/MS) for performing mass spectrometry of fractionated samples prepared with a multi-dimensional LC or similar LC with high separatory capability, fractionated sample useful information which shows the degree of usefulness of various substances with respect to retention time is prepared from prior information which includes, for example, elution characteristics in the LC depending on the kind of column or other factors or the degree of ease of ionization in the MS. During a measurement, the preparative separation of an eluate and the preparation of fractionated samples are not performed within a period of time which has been judged to be useless based on the fractionated sample useful information. By selecting fractionated samples at each dimension of the multi-dimensional LC, the number of fractionated samples to be eventually subjected to mass spectrometry can be significantly reduced.
    Type: Grant
    Filed: September 8, 2014
    Date of Patent: December 31, 2019
    Assignee: SHIMADZU CORPORATION
    Inventors: Shinichi Utsunomiya, Yusaku Hioki, Yuki Ohta
  • Patent number: 10501862
    Abstract: A plating system comprising a plating tank for applying plate processing to a substrate, a sensor configured to measure actual plating film thickness of the substrate, and a controller configured to control plating current supplied to the plating tank and plating time for the plate processing of the substrate within the plating tank. The controller is capable of setting target plating film thickness, plating current, and plating time as a plate processing recipe. At least one of the plating current and the plating time is automatically corrected so that the actual plating film thickness and the target plating film thickness become equal to each other, and the result is reflected in the plate processing for the subsequent substrate.
    Type: Grant
    Filed: August 4, 2017
    Date of Patent: December 10, 2019
    Assignee: EBARA CORPORATION
    Inventors: Takashi Mitsuya, Ryuya Koizumi, Toshio Yokoyama, Masashi Shimoyama, Kunio Oishi
  • Patent number: 10494449
    Abstract: Polydisperse and charged polysaccharides are fractionated into low polydispersity fractions (preferably having pd<1.1), each containing species within a narrow range of molecular weights. An aqueous solution of the polydisperse polysaccharides is contacted with an ion exchange resin in a column and the polysaccharides are subjected to selective elution by aqueous elution buffer. The selective elution consists of at least 3 sequential elution buffers having different and constant ionic strength and/or pH and in which the subsequent buffers have ionic strength and/or pH than those of the preceding step. The new preparations are particularly suitable for the production of PSA-derivatised therapeutic agents intended for use in humans and animals.
    Type: Grant
    Filed: November 22, 2017
    Date of Patent: December 3, 2019
    Assignee: Lipoxen Technologies Limited
    Inventors: Sanjay Jain, Ioannis Papaioannou, Peter Laing