Abstract: This invention relates to an analytical method and an apparatus utilizing a SPE cartridge, which can realize enhanced accuracy and workability in the analysis of trace substances, such as residual pesticides, can clean the inside of piping, needle valves etc, can eliminate the influence on the analysis and can conduct these operations automatically. In the analytical method and apparatus utilizing a SPE cartridge, analytes previously adsorbed to a SPE cartridge is eluted with a solvent from a liquid feed pump. The eluate was injected into a storage chamber in a gas chromatograph through a needle inserted into an injection port in the storage chamber. The needle is pulled up from the injection port, and the inside of the needle is washed with a solvent fed from the liquid feed pump. The wash liquid is discarded, and the analysis of organic compounds as analytes in an subject sample is carried out.

Abstract: It is an object of the present invention to improve the performance of gradient liquid transfer in nano/micro liquid chromatographs when solvents are mixed. In a gradient liquid transfer device and method for a nano/micro liquid chromatograph, an opening and closing unit (160) capable of opening and closing a solvent passage (150) for transferring a secondary solvent is provided in the vicinity of a solvent mixing section (152) in a liquid transfer system (130); a passage from a metering pump (146) of a liquid transfer section (142) to the opening and closing unit (160) is filled with the secondary solvent in advance; and an appropriate pressure is applied to the secondary solvent. Therefore, the entry of a primary solvent into the solvent passage for transferring the secondary solvent is suppressed.

Abstract: Pretreatment method and apparatus to remove matrix ions from a liquid sample, prior to separation of the sample analytes (e.g. by chromatography), by flowing the liquid sample into a sample compartment and stopping the flow. The sample compartment has a wall comprising an ion exchange membrane having exchangeable ions of the same charge as the matrix ions to be removed. A regenerant liquid stream flows through a regenerant flow compartment on the other side of the membrane from said parked liquid sample. Matrix ions in the parked liquid sample are transported across the membrane into the regenerant flow compartment. Suppression may be performed electrolytically and/or chemically. A concentrator column may also be used.

Abstract: Disclosed is a simulated moving bed (SMB) adsorptive separation system in which at least one component of a fluid mixture is brought into contact with a solid adsorbent and the adsorbed component is desorbed with a desorbent, wherein the improvement comprises an inert ball layer is formed on the upper part of the solid absorbent filled in the simulated moving bed.

Abstract: Gradient performance with high pressure gradient solvent delivery system is optimized by approximation of infinite stroke volume of high pressure pumps by the addition of pulse dampening with backflow prevention to each high pressure pump. The backflow prevention adds sufficient minimum flow resistance, thereby enhancing the performance of the pulse dampening over a wider range of flow rates resulting in consistent gradient performance.

Abstract: A method of managing the valves of a simulated moving bed separation system comprising at least one column of adsorbent beds split into several zones delimited by injection and draw-off points, allowing finer monitoring of a determined concentration profile. The method comprises, during each period between the shift times of the injection and draw-off points, in opening and closing a first valve located at one end of a zone according to a determined law, while opening and closing the valve downstream from the first valve in relation to the direction of flow of the recirculation flow, according to the same law with a lag, referred to as overlap time, in relation to the first valve, which is less than or equal to the period, the law imposing a constant total flow rate for the flow passing through the two consecutive valves throughout the overlap time.

Abstract: The invention concerns a simulated moving bed (SMB) separation device and process comprising a column, beds Ai of adsorbent separated by plates Pi with a single distribution and extraction network for fluids, in particular feed F, desorbant D, raffinate R and extract E, and a plurality of two-way valves for distribution of said fluids, said valves being limited in number and in particular with dimensions that are smaller than in the prior art. According to the invention, the column is divided into a plurality of sectors Sk with 2 superimposed plates, each sector Sk comprising an external principal bypass line Lk connected to each plate Pi of Sk via a large diameter plate valve Vi and an external secondary bypass line Mk comprising a small diameter valve VMk connected to the adjacent sector Sk?1.

Abstract: A fraction collector comprising a movable tray is provided. The movable tray may be attached to the fraction collector support system in a variety of ways and adjusted without adjusting the parts of the fraction collector support system. The moveable tray may be stably attached to the fraction collector support system by either fixed or removable pegs, as well as by channels.

Abstract: The present invention relates to a method of separating sugars and sugar alcohols from each other. More particularly the present invention relates to the use of a weakly basic anion exchange resin in a chromatographic separation process. The advantage of the present invention compared with the prior art is that it is especially suitable for separating reducing sugars in acidic conditions as well as for example in weakly acidic conditions. The method using chromatographic separation comprises at least one step where a weakly basic anion exchange resin is used in a chromatographic column or in a part of a column.

Abstract: A liquid chromatograph has switching valves as a flow passage switching mechanism and an automatic sampler as an injection section. The liquid chromatograph can switch, by switching the switching valves between a concentration flow passage for concentrating the sample injected by the automatic sample via a flow passage which connects the automatic sampler to the trapping column; a concentration analysis flow passage for separating and analyzing the concentrated sample on a per-composition basis which connects the trapping column to the analysis column; and a direct analysis flow passage by separating the sample on a per-composition basis which connects the automatic sampler to the analysis column.

Abstract: A method and apparatus for monitoring and controlling the nano-scale flow rate of fluid in the operating flow path of a HPLC system without relying on a nano-scale sensor in the operating flow path. A main flow sensor is disposed in the main flow path between the pump and a flow-divider. A waste flow sensor is disposed in the waste flow path downstream of the splitter. The output signal of the waste flow sensor is subtracted from the output signal of the main flow sensor in a difference circuit. The difference signal is divided by the output signal from the main flow sensor in a divider circuit. The output of the divider circuit represents an empirical split ratio of the flow-divider and is independent of media composition.

Abstract: Disclosed is a mixer-distributor-collector apparatus for use between beds of solid particles in a fluid-solid contacting vessel. The apparatus includes a solids retaining screen, fluid deflector, passageway, fluid distributor, and flow manipulator. The flow manipulator is a device such as a honeycomb, porous solid, perforated plate, screen, or grid having an open area greater than the open area of the fluid distributor and is located below and spaced apart from the fluid distributor. The apparatus improves the fluid flow characteristics by minimizing or eliminating fluid velocity jets and/or other turbulence which can disturb the downstream particle bed. In an exemplary application, the invention finds use in simulated moving bed (SMB) adsorptive separation processes.

Abstract: In the method for mixing-free transport of homogeneous liquids in microchannels a heterogeneous liquid flow (14) is autonomously divided into portions, transported over a distance and then autonomously recombined. When the flow is divided into portions, individual volumes of a second liquid (16), which may be but must not be homogeneous, are introduced between volume fractions lying one behind the other of the heterogeneous liquid flow (14). While the volume fractions of the heterogeneous liquid (14) that lye close to one another would mix with each other without this measure being taken, separation of the individual volume fractions allows these volume fractions, and thus the heterogeneous liquid (14), to be transported over large distances without any mixing occurring.

Abstract: The present invention provides a method having a high productivity for preparative separation by simulated moving bed chromatography. That is, in a filler for enantiomeric isomer separation by simulated moving bed chromatography, which has a porous carrier carrying a polysaccharide derivative thereon and has a TS coefficient in a range of 0.55 to 1.20, the TS coefficient being defined by the below-shown formula (I), and obtained by using an enantiomeric isomer separation column for simulated moving bed chromatography in which the filler is filled in a column tube by a slurry filling method: TS coefficient=[Vc?[t(TS)?t(blank)]×FR]/[t(TS)?t(blank)]×FR,??(I) wherein Vc (cm3): a column volume; FR (ml/min.): a flow velocity; t(TS) (min.): an elution time for tetrakis(trimethylsilyl)silane (=TS); and t(blank) (min.): an elution time for TS in the state where the column is not connected.

Abstract: Device and process for separating at least one compound from a mixture in a simulated moving bed, comprising: an enclosure comprising several absorbent beds, two adsorbent beds being separated by a fluid distribution and extraction plate (Pi), the plate comprising one or more distribution, mixing and/or extracting panels, comprising a chamber (Ci), main fluid delivery (4) and extraction (2) lines, several secondary fluid extraction or injection lines (10, 11, 12, 13, Ti), a bypass circuit communicating a distribution plate with a bypass line, means (14, Voi,j, 20) communicating at least one chamber (Ci) with at least one bypass line (Li,j), at least one of the ends of a bypass line communicates with a zone Ri, R?I of an adsorbent bed, said zone being distinct from a distribution chamber (Ci) and another end is connected to said chamber (Ci).

Abstract: There is described a chromatographic separation member including a substrate carrying on at least one surface a thin layer of a chromatographically active capture material. Also described is a chromatographic separation method for separating a component of interest from a fluid utilizing the chromatographic separation member.

Abstract: A method for fractionating a solution into two or more fractions. More particularly the invention relates to a method for fractionating a solution by a chromatographic simulated moving bed (SMB) process in which the dissolved substances present in the feedstock are separated in the partial packed beds, and the formed separation profile is circulated more than once or less than once through the chromatographic separation loop during one cycle.

Abstract: Device for improving detection sensitivity in low flow velocity high performance liquid chromatographic apparatus. Preferredly, diffusion promoting device connected at just before separation column and having function of improving detection sensitivity for use in low flow velocity high performance liquid chromatographic apparatus. Also, method for improving detection sensitivity in low flow velocity high performance liquid chromatographic apparatus.

Abstract: Provided are methods of separating an immunoreactive compound from at least one immaterial component, using a simulated moving bed (“SMB”) system and a SMB apparatus for use in these methods. Also provided are purified immunoreactive compounds prepared using the SMB methods and apparatus and methods of treatment with the purified immunoreactive compounds.

Abstract: The present invention relates to a method and apparatus for chromatographically analyzing each of a plurality of samples in detector, comprising an autosampler to contain a plurality of samples for chromatographic analysis and a plurality of chromatographic systems, each system comprising one or more pumps and one or more chromatography columns. A detector is included for detecting compounds in the samples from each of the chromatography systems along with a valve positioned between the detector and the plurality of chromatography systems, the valve permitting each sample to reach the detector in sequence. A computer control device is included which adjusts the introduction of samples from the autosampler into the plurality of chromatography systems as well as the position of the valve to sequentially separate and deliver compounds within the samples to the detector.

Abstract: Method of optimizing the operation of a unit intended for separation of the components of a feed (xylenes) by simulated countercurrent in hybrid operating mode. The method allows to minimize the solvent ratio and to maximize the capacity of the separation unit while keeping product specifications such as purity and yield constant. It has been verified that these two objectives cannot be reached simultaneously and it is recommended to operate with a minimum solvent ratio while guaranteeing a high capacity compatible with stable operation of the separation unit. These optimization objectives are reached while keeping good stability around the optimum point thus defined, by using a known operation control process such as the one described in patent EP-875,268 for example.

Abstract: A method for separating one or more components of a biomolecule mixture by means of an ion exchange chromatographic system operated in the displacement mode includes sequentially perfusing the system with a first solution including the biomolecule mixture, and a second solution including a displacer having a structure selected from formula I and formula II:

Abstract: A method and apparatus for monitoring and controlling the nano-scale flow rate of fluid in the operating flow path of a HPLC system. A first flow sensor is disposed in a first flow path between a first flow-divider and a fluidic tee. A second flow sensor is disposed in a second flow path between a second flow-divider and the fluidic tee. A first recycle flow restrictor is disposed in the first recycle flow path in fluid communication with the first flow-divider. A second recycle flow restrictor is disposed in the second The permeability of each recycle flow restrictor can be selected to produce a desired flow rate with each respective flow path. The output signals of the first and second flow sensors to control output of a pump within each flow path.

Abstract: A liquid chromatograph control apparatus (1) includes a measured value storage (25a) that stores a measured retardation factor (Rf) value of a sample (3), which is obtained when components of the sample are separated on thin layer chromatography using eluent (4) containing a plurality of ingredients at a specified mixture ratio, in association with the specified mixture ratio, a rate-of-change-in-Rf-value storage (25b) that stores a rate of change in the Rf value of the sample (3) with respect to variation in mixture ratio of ingredients of eluent (10), a mixture ratio calculator (22a) that determines a mixture ratio of the eluent (10) at which a specified retardation factor value (RfO) of the sample is obtained, based on the measured Rf value corresponding to the specified mixture ratio stored in the measured value storage (25a) and on the rate of change in the Rf value stored in the rate-of-change-in-Rf-value storage (25b), and a mixture ratio controller (22b) that outputs a control signal to control the mi

Abstract: In accordance with one aspect of the present invention, an ultra-high pressure chromatography system (UHPLC) is provided and includes a high pressure chromatography arrangement of equipment (HPLC system) as well as a UHPLC module for converting the HPLC system into the UHPLC system. One exemplary module includes a gradient storage column fluidly connected to the HPLC equipment in a post injector manner such that an injection plug can be selectively delivered and stored, in an inverted manner, therein at low pressures, while stored injection plug (gradient) is delivered to an analytical column using higher pressures for chromatographic separation.

Abstract: Distributed valve simulated moving beds are described in which junctions located between successive columns interrupt the flow of process fluid between columns, and either transmit the process fluid through a zone bypass to a succeeding column within the same zone or, if the junction is located between zones, direct the process fluid to an input/output line that is dedicated to the particular zone that immediately follows the junction. At each step of the SMB's operation, the distribution of flows in each junction is modulated to accomplish movement of ports consistent with the SMB's design. Further described are simulated moving beds that contain one or more decoupled on-line regeneration zones. The regeneration zone is decoupled from the separation zones in the sense that it observes a step time (a “regeneration interval”) that is different than the step time observed by the separation zones of the SMB.

Abstract: In one aspect, the invention provides a method for separating a mixture of polynucleotides, such as DNA or RNA, including (a) applying the mixture to a polymeric separation medium having non-polar surfaces, wherein the surfaces are characterized by being substantially free from multivalent cations, such as metal ions, which are free to interfere with polynucleotide separation, and (b) eluting the mixture with a mobile phase containing organic solvent and counter ion agent. In the separation of single-stranded polynucleotides, improved separation is obtained at a temperature effective to fully denature secondary structure within the polynucleotides.

Abstract: In a preferred embodiment, a sample container storage part for storing a number of sample containers S, a nozzle for dropping a sample component separated and supplied by an LC and an additive liquid such as digestive fluid supplied from another liquid supplying part to the sample container S, a carrying mechanism for carrying and positioning the sample container at an arbitrary position under the nozzle, and a second nozzle, serving as a suction/injection mechanism, for sucking in the fractionated/collected sample component and injecting the sample component to another LC. The carrying mechanism provides a rotation mechanism. The carrying mechanism rotates over 180 degrees and carries the sample container S completed with fractionating/collecting to the position of the second nozzle, and the sample is sucked in by the second nozzle and injected to the LC of next stage.

Abstract: The invention relates to an equipment for fractionating a solution by a sequential SMB process, which equipment comprises a feed column and one or several other columns, which include a chromatographic separation resin bed. The equipment of the invention is characterized in that the feed column consists of or comprises a compartment where the resin bed is shallower than that of one or more of the other columns of the equipment. The invention also relates to a method for fractionating a solution into two or more fractions with the equipment of the invention.

Abstract: A solid bed adsorptive process for separating a seed oil into two substantially pure triglyceride fractions. The process involves contacting a seed oil, such as castor oil, preferably as a concentrate, with an adsorbent in a bed, the adsorbent having a particle size greater than about 40 microns, and thereafter contacting the adsorbent with a desorbent material, preferably under minimal flow conditions, to obtain a raffinate output stream containing predominantly a second triglyceride and an extract output stream containing predominantly a first triglyceride. Purified fatty acid triglyceride esters obtainable from castor, vernonia, and lesquerella plant oils provide renewable, non-petroleum-based sources of chemical feedstocks.

Abstract: The invention relates to a method for determining the binding behavior of ligands which specifically bind to target molecules at at least one binding site, whereby the markers are present in a native form and the determination of the concentrations K4 and K5 or of the quantities M2 and M1 is carried out by means of mass spectrometry.

Abstract: The invention relates to a system and a process for fractionating a solution into two or more fractions. The system of the invention comprises at least two compartments having a diameter of at least about one meter and including a uniform packing of a polymer-based ion exchange resin with a bead size in the range of about 50 to about 250 ?m. The mixing volume of the fluid fronts in the system of the invention is not more than 5% of the volume of the compartment.

Abstract: The present invention relates to a method of fractionating liquid mixtures or solutions, comprising the steps of subjecting a feed in the form of a liquid mixture or solution to a first chromatographic separation, thereby recovering at least a fraction (A) and a fraction (B) both containing a first component or product, subjecting a mixture or solution derived from or comprising said fraction (A) to a second chromatographic separation, thereby recovering at least a fraction (C) rich in said first component or product and subjecting a mixture or solution derived from or comprising said fraction (B) to a third chromatographic separation, thereby recovering at least a fraction (D) also rich in said first component or product, and whereby at least two fractions are recovered from the second and third chromatographic separations.

Abstract: The present invention relates to a method and apparatus for chromatographically analyzing each of a plurality of samples in detector, comprising an autosampler to contain a plurality of samples for chromatographic analysis and a plurality of chromatographic systems, each system comprising one or more pumps and one or more chromatography columns. A detector is included for detecting compounds in the samples from each of the chromatography systems along with a valve positioned between the detector and the plurality of chromatography systems, the valve permitting each sample to reach the detector in sequence. A computer control device is included which adjusts the introduction of samples from the autosampler into the plurality of chromatography systems as well as the position of the valve to sequentially separate and deliver compounds within the samples to the detector.

Abstract: A method and apparatus for the continuous deposition of RP HPLC eluent, from isocratic or gradient elutions, on to a MALDI target using a parallel sample desalting method, allows increased sensitivity of the MALDI sample by pre-concentration of the analyte, separation of interfering contaminants from the analyte and increased dynamic range of peptide abundance that can be analyzed. The parallel processing of sample material allows greater throughput than that of manual methods.

Abstract: The invention encompasses systems for column-based separations, methods of packing and unpacking columns and methods of separating components of samples. In one aspect, the invention includes a method of packing and unpacking a column chamber, comprising: a) packing a matrix material within a column chamber to form a packed column; and b) after the packing, unpacking the matrix material from the column chamber without moving the column chamber.

Abstract: An apparatus is presented for separating chemicals using adsorption separation methods. The apparatus uses a plurality of adsorption units holding adsorbent, where the adsorption units positioned in a cylindrical spool, are serially connected and the spool is rotated to shift the relative position of the feeds and drawoffs to the apparatus.

Abstract: A hand held, self-contained, automatic, low power and rapid sensor platform for detecting and quantifying a plurality of analytes. A sample solution potentially containing an unknown amount of an analyte is passed through an affinity column which contains antibodies to which the analyte binds thereby extracting the analyte. The affinity column is then rinsed to remove any other chemicals that may fluoresce. The rinsed affinity column is then eluted with a known volume of elution fluid causing the analyte to release from the antibody and dissolve in the fluid (eluant). The eluant is then placed in the quartz cuvette of a fluorometer. The analyte suspended in the eluant fluoresces at a waveband which is different than that of the light source that excites it. The amount of fluorescence is measured and the level of analyte determined.

Abstract: A process and apparatus for purifying one or more target substances from a source liquid, employing one or more cross-flow filter elements, and one or more types of chromatography resins, in combination, to provide purification with advantageous yield, product purity, and cost- and time-efficiency.

Abstract: A liquid chromatograph mass spectrometer comprises: a pump; a sample injector; a plurality of separation columns, including a first separation column and a second separation column; and mass spectrometer.

Abstract: Method allowing control of an evaporative light scattering detector which is coupled to a liquid chromatography column, substantially independently of the elution conditions for the chromatographic separation. A given adjustable constant calibrated volume of the flow which originates from the chromatography column is removed at a given adjustable frequency, this flow being constituted by an eluent which contains compounds to be analysed which have been dissolved therein, and the volume removed in this manner is transferred to a secondary circuit, to which the evaporative light scattering detector is connected, by being conveyed by means of an auxiliary pump with a specific carrier fluid which is independent of the eluent having a predetermined flow, and the successive fractions of the flow which originates from the chromatography column are mixed with the carrier fluid which conveys these fractions upstream of the evaporative light scattering detector.

Abstract: A method for separating one or more components of a biomolecule mixture by means of an ion exchange chromatographic system operated in the displacement mode includes sequentially perfusing the system with a first solution including the biomolecule mixture, and a second solution including a displacer having a structure selected from formula I and formula II:

Abstract: A polymeric sorbent that can be employed in the extraction and purification of polar and nonpolar molecules from a complex media (e.g. pharmaceuticals from biological matrices) by solid phase extraction (SPE). The sorbent exhibits a strong capacity for the retention of polar molecules and can facilitate the recovery of compounds possessing a range of polarities while furnishing clean extracts showing low ion suppression. The polymer is wettable and remains wetted over long periods of time.

Abstract: The present invention provides a method and device for collecting and concentrating a desired compound from a chromatographic column exit stream. The collection chamber can be heated or cooled and washed in a forward or backward direction for efficient removal.

Abstract: Fluctuations, such as perturbations in the quality of the extract and/or raffinate streams removed from the circuit in a simulated moving bed (SMB) adsorptive process having at least one discrete non-separating section are eliminated or significantly reduced. This is accomplished by increasing the fluid flow rate in a portion of the circuit in response to the location of a discrete non-separating section and changing the flow rate of at least one stream that is in fluid communication with the circuit consistent with the increased flow rate in the portion of the circuit. Multiple adjustments to the flow rates of the input and/or output streams and multiple increases in the fluid flow rates in portions of the circuit may be used simultaneously when for example there is more than one discrete non-separating section.

Abstract: A method for fractionating a solution into two or more fractions. More particularly the invention relates to a method for fractionating a solution by a chromatographic simulated moving bed (SMB) process in which the dissolved substances present in the feedstock are separated in the partial packed beds, and the formed separation profile is circulated more than once or less than once through the chromatographic separation loop during one cycle.

Abstract: A method for fractionating a solution into two or more fractions by a chromatographic simulated moving bed (SMB) process, wherein the separation system comprises at least two separation profiles in the same loop.

Abstract: Disclosed is a process for dewatering an organic compound other than a carboxylic acid using a simulated moving bed (SMB) containing a solid with different absorptivities for water and the organic compound. The organic compound is fed into the SMB as an aqueous solution and desorbed with a desorbent other than water. The process is particularly useful for dewatering organic compounds produced by fermentation, biomass extraction, biocatalytic, and enzymatic processes.

Abstract: A rapid analysis method for the determination of polymer average molecular weight separates the polymer's high molecular weight fraction and determines its average molecular weight using a molar mass detector and a concentration detector. The method avoids time consuming sample preparation, allows for separation of interfering low molecular weight components, and is suitable for automation.

Abstract: Methods and apparatus for characterizing a polymer sample and in preferred embodiments, libraries of polymer samples, in a comprehensive, directly-coupled multi-dimensional liquid chromatography system are disclosed. The first and second dimensions are preferably high-performance liquid chromatography dimensions, such as for example, a first dimension adapted for determining composition (e.g. adapted for mobile-phase gradient elution chromatography, including reverse phase chromatography, adsorption chromatography and the like), and a second dimension adapted for determining molecular weight or particle size (e.g., adapted for size exclusion chromatography, including gel permeation chromatography).