Abstract: A serum or plasma collection device including a gel material for partitioning and separating heavier and lighter phases of a fluid sample such as a blood sample. The device preferably includes an affinity member such as a disc-shaped or ring-shaped member or a coating on the collection tube which has an affinity for the separated oil or degradation material from the gel material to prevent the separated material from interfering with the sampling of the separated fluid sample. The affinity member is preferably formed of hydrophobic and/or non-wetted material and has a specific gravity different than the specific gravity of the lighter phase.

Abstract: A system for collecting and processing donated blood comprises a first porous medium interposed between a blood collection bag and a satellite bag and a second porous medium interposed between the blood collection bag and another satellite bag. The porous media are leucocyte depletion media. The system may also include one or more of the following: a red cell barrier medium, a separation medium, a gas inlet, and a gas outlet. The system can be used to centrifuge whole blood into one or more components, and includes a means for protecting the system during centrifugation.

Abstract: A blood collection tube includes a bottom and an open end that is hermetically sealed by a plug. A supporting film having an anticoagulant coated thereon is positioned within the tube. The anticoagulant-coated surface of the supporting member is provided with a large number of fine convexes.

Abstract: The device for separating plasma from a sample of whole blood by centrifuging comprises:a distributor-divider disposed centrally and provided with n compartments communicating with a sample-receiving well and communicating with one another via notches;a ring separator including n receptacles communicating with the n compartments and with n outer cells and terminating in an open funnel; anda removable plasma collector fitted onto said funnel.

Abstract: An analytical rotor for separating cellular components from a biological fluid includes a separation chamber spaced radially outward from a sample chamber. The sample chamber may be an open receptacle disposed to receive sample or may be a mixing chamber which receives sample and diluent. A flow restrictive channel connects the sample chamber to the separation chamber so that fluid enters the separation chamber at a controlled rate. The cellular components collect within a retention region located generally at the outer periphery of the collection chamber while cell-free fluid is continuously removed through a collection port. The collection port is spaced annularly apart from the flow channel so that there is sufficient residence time within the separation chamber for substantially complete separation of the cells from the fluid fraction.

Abstract: The present invention provides a centrifugal rotor which measures and delivers a predetermined volume of fluid to a receiving chamber in the rotor. The rotor comprises a bulk fluid chamber containing a bulk amount of fluid and a metering chamber which has a predetermined volume. The bulk fluid flowing into the metering chamber fills the chamber and excess fluid flows out into an overflow chamber. Thus, a predetermined volume is obtained. This volume is delivered to the receiving chamber through an exit duct which prevents flow at the rotational speed required to fill the metering chamber but allows flow at a second, higher rotational speed. The exit duct is typically a capillary in which capillary forces prevent flow until the rotational speed is increased.

Abstract: Method and apparatus are provided for machine separation of blood into blood components, wherein a preselected fluid such as anticoagulant is added to at least two different locations through the fluid flow path of the set, in order to promote the different functional characteristics in different blood components. The method results in reduced incidence of citrate reaction in the blood donor and increased platelet yield in platelet rich plasma collected during the procedure.

Abstract: A modular reaction cassette has a fluid metering mechanism and valves for releasing fluid which are actuated by various centripetal acellerations generated in an automated patient analysis instrument having a variable speed rotor. Various geometries and sealant materials are used to select the rotational speed at which the sealant material yields to operate the metering device and release valves.

Abstract: A serum separation sealant containing 0.1 to 20 parts by weight of fine resin particles having an average particle size of 0.01 to 2 .mu.m and having an internal crosslinking density of 0.1 to 3 mmol/g and 100 parts by weight of a gelatinous material.

Abstract: Plastic blood bag system including a bag having a generally longitudinal seal extending from the top of the bag and between two blood bag ports downwardly to at least the lower half of the bag but not to the bottom of the bag, thereby leaving the sole passageway within the bag and between two ports located in the lower half of the bag.

Abstract: The invention discloses a method for treating blood comprising (a) centrifuging blood and (b) passing a supernatant layer of the centrifuged blood through a porous medium until red blood cells block the porous medium.

Abstract: Quantification of blood plasma fibrinogen content is made in a sample of anticoagulated whole blood contained in a sampling tube. The tube is a transparent centrifuge tube which contains a sample constituent layer-elongating float. The blood sample is centrifuged in the tube containing the float, and various cell constituent measurements are made. The centrifuged sample is then heated and recentrifuged to cause the precipitated fibrinogen to layer out on top of the float remote from the buffy coat band in the plasma layer. The thickness of the fibrinogen band is then measured, whereby quantification of the fibrinogen content of the blood sample can be made by a precalibrated instrument.

Abstract: Method and apparatus for machine separation of blood and blood components that utilizes improved administeration of anticoagulant to the red cell suspension output. Anticoagulant is added to incoming whole blood in an amount sufficient to prevent clotting of the whole blood in the flow path upstream of the blood component separator, but in an amount insufficient to prevent clotting of the red cell suspension created by the separator. Anticoagulant is added to the red cell suspension downstream of the separator in order to prevent clotting. By adding an additional aliquot of anticoagulant to the red cell suspension, the amount of anticoagulant added to whole blood before separation can be reduced or eliminated, resulting in an increase in platelet yield during the blood component separation step.

Abstract: Systems and methods of collecting blood cells use a first container connected to initially collect blood. A filtration system is provided that uses two flow paths, the first being a dual purpose path that is initially used to convey plasma and platelets to a pair of transfer/storage containers. A second flow path leads to a temporary transfer container through a filtration device. The dual purpose fluid path is next used to convey blood cells, now substantially free of undesired matter, from the temporary storage container to a storage container. The filtration system is then detached from the blood collection system.

Abstract: A filter system for separating and binding biomolecules contained in a solution includes a first filter means for selectively passing components of the solution that are smaller than the biomolecules, thereby concentrating the biomolecules in the solution. Also included is a binding membrane means having an affinity for the biomolecules for binding the biomolecules to the binding membrane means, with the binding membrane means being located where the biomolecules are concentrated by the first filter means. In the preferred mode, the binding membrane means is chosen to selectively bind the biomolecule of interest. According to a preferred method of the invention, biomolecules from a sample volume are bound to a binding membrane by urging the sample volume against a biomolecule-binding support membrane that is in contact with a cut-off filter membrane such that the sample volume will pass through the biomolecule-binding support membrane before passing through the cut-off filter membrane.

Abstract: A centrifugal rotor comprising a plurality of peripheral cuvettes and a collection chamber spaced radially inward from the cuvettes is disclosed. The rotor includes a means for introducing fluid into the collection chamber and plurality of generally radial inlet channels connecting each cuvette to the chamber. Each inlet channel has a discrete flow path for fluid to enter the cuvette and another discrete flow path for gas to exit the cuvette as the cuvette is filled. Positioned radially inward from each cuvette is a reflective surface capable of deflecting a light beam from a generally vertical direction to a generally horizontal direction or vice versa. As the rotor is spun, fluid enters the cuvettes from collection chamber through the inlet channels, which also allow gas in the cuvettes to escape, thus avoiding the creation of bubbles in the cuvette after the cuvettes are filled. The contents are then optically analyzed by passing a light beam horizontally through the cuvette.

Abstract: Red Blood Cells (RBC's) are removed from whole blood samples by contacting the whole blood sample with a solution containing an acid selected from the group consisting of acetic acid, citric acid, ascorbic acid, lactic acid, maleic acid, malic acid, and malonic acid. The agglutinated RBC's are then removed from the resultant suspension by procedures of filtration, centrifugation or decantation, leaving an essentially RBC-free serum or plasma sample.

Abstract: An apparatus for dispensing a predetermined amount of a sample into a fluid. The apparatus comprises a chamber which may be inserted into a cuvette. The chamber comprises a means such as a membrane or filter ball for dispensing a predeterming amount of a sample into a fluid upon contact of said membrane or filter ball and said sample by said fluid. The fluid is brought into contact with the sample by vortexing the fluid with a vortex mixer.

Abstract: A method of preparing neocytes and gerocytes in a closed blood bag system useful for the filtration of leukocytes from a red blood cell concentrate followed by the preparation and long term storage of neocytes and, preferably, gerocytes from the filtered concentrate. In very preferred embodiments, the system also provides containers for the expression of plasma and the preparation and long term storage of platelets.

Abstract: A system for collecting and processing donated blood comprises a first porous medium interposed between a blood collection bag and a satellite bag and a second porous medium interposed between the blood collection bag and another satellite bag. The porous media are leucocyte depletion media. The system can be used to centrifuge whole blood into one or more components, and a means for protecting the system during centrifugation including a bracket carried by the centrifuge bucket for receiving the porous media.

Abstract: Filtration of white blood cells (WBCs) directly from platelet rich plasma (PRP) rather than from subsequently made platelet concentrates (PC). Earlier filtration is effective at removing most WBCs with minimal platelet loss and permits storage of platelets for at least 5 days. The filtration is preferably done with a closed, ready-to-use system comprising a WBC filter disposed between a first blood bag and at least two empty secondary bags. A fourth bag may be included in the closed system to contain a platelet additive solution. System can remove at least 95% of the WBCs with less than 10% loss in platelets. In very preferred embodiments at least 99.5% of the original WBCs are removed by filtration prior to platelet storage.

Abstract: A system and method of separation of therapeutic components from blood. The system includes a dual member centrifuge and a disposable single use fluid transfer set. The set includes an elongated flexible separation chamber having an input port, a separated component output port and a residual fluid output port. The input port and the separated component output ports are located at opposite ends of the elongated separation chamber. The centrifuge includes a receiving chamber with a selectively formed annular slot therein. The separation chamber is positioned in the annularly shaped slot and rotated at a predetermined rotational velocity. Fluids such as whole blood flows through the separation chamber and are separated into various therapeutic components such as platelet rich plasma and residual concentrated red blood cells. Platelet rich plasma can be drawn off as the separated therapeutic component. An alternate two part transfer set provides for highly efficient platelet pheresis.

Abstract: A method for the separation of blood into the components thereof, characterized by subjecting the blood to strong centrifugation thereby separating said blood into an upper layer (A) of platelet-deficient blood plasma an intermediate layer (B) of a mixture of platelets and white blood corpuscles, and a lower layer (C) of a red blood corpuscle concentrate, adjusting the hematocrit value of said red blood corpuscle concentrate of (C) to not more than 80% by diluting said red blood corpuscle concentrate with part of said platelet-deficient blood plasma of (A), and subjecting said mixture of (B) to weak centrifugation thereby separating said mixture into a lower layer of white blood corpuscles and an upper layer of a platelet concentrate, and if desired, subjecting the remaining platelet-deficient blood plasma to the treatments of freezing, thawing, and centrifugation thereby separating said platelet-deficient blood plasma into cryoprecipitate and cryoprecipitate-deficient blood plasma, and apparatus therefor.

Abstract: A device for obtaining from at least two spatially separated areas the heterogeneous components of a liquid, especially stabilized whole blood in a flexible and transparent bag with at least one line leading out of it. The device consists of a front plate that is part of a housing, of a pressure-application plate that pivots toward the front plate, of a drive mechanism, and of a sensor. The point that the pressure-application plate pivots around is below the bag and at the point of intersection between the surfaces of the plates, specifically at least 30% of the length of the pressure-application plate away, and the drive mechanism engages a lever that is rigidly secured to the pressure-application plate. A bag employed in conjunction with the device has an intake line at the top and an outlet line and a filling and blood-collecting tube at the bottom, only one bag-to-bag transport system at the top, and no outlet connections.

Abstract: An integrated blood collection, separation and dispensing device is disclosed that allows a method of blood collection, serum separation and serum dispensing to occur using a single device (other than a centrifuge). The device preferably includes a transfer chamber for receiving separated serum, that has a three-dimensional shape that is reduced in volume and is configured to avoid air entrapment. A dispensing aperture in the transfer chamber fluidly connects the chamber with the atmosphere. Preferably, means are provided adjacent the dispensing aperture for generating a liquid pressure change indication of the presence of liquid advancing towards the dispensing aperture. A needle and a protective housing optionally are provided as an integral part of the device.

Abstract: A device is disclosed that causes phase separation of whole blood, using much lower centrifugal forces. As a result, lymphocytes are separated from blood cells having specific gravities of 1.08 g/ml or higher. The device features a separation chamber arranged so that its long dimension or axis is parallel, not perpendicular, to the spin axis, and a valve that allows automatic removal of the lighter phase(s). The valve is constructed to respond only to the head of liquid pressure generated by an increased centrifugal force, and not to that increased force alone.

Abstract: A method of use is disclosed of a device that causes phase separation of whole blood, using much lower centrifugal forces. As a result, leukocytes are separated from blood cells having specific gravities of 1.09 g/ml or higher. The method preferably uses a separation chamber arranged so that its long dimension or axis is parallel, not perpendicular, to the spin axis, and a valve that allows automatic removal of the ligher phase(s).In one aspect, the method separates a lighter phase from a heavier phase, for example, lymphocytes from whole blood, using a centrifugal force of reduced amount. In another aspect, DNA is extracted readily from the separated lighter phase since it is less likely that the lighter phase will be contaminated by erythrocytes as occurs in conventional separation techniques.

Abstract: Apparatus and a method are described, wherein a coagulator accelerator is added to a container to achieve agglutination of blood cells when centrifuging whole blood, allowing the serum to be more easily poured off. The accelerator is selected from polymeric amines that are either polymerized amino acids or vinyl addition polymer amines, with a specified molecular weight range, the vinyl addition amines being selected to avoid lysing the blood cells.

Abstract: A method for separating blood into blood components aseptically in a closed system first filters whole blood through a filter for removing leukocytes or removing leukocytes and platelets, to produce filtered blood, which is passed to and collected in a primary bag fluid-tightly connected to the filter through a sealable, cuttable conduit, and the conduit is sealed and cut to disconnect the primary bag from the filter. The disconnected primary bag is subjected to centrifugation to separate the filtered blood into blood components. The method is useful for separately collecting leukocyte-removed blood components, particularly leukocyte-removed erythrocytes, leukocyte-removed plasma, leukocyte-removed platelets, etc., from the whole blood of a healthy human. A blood components separation unit which can advantageously be used in the practice of the above method is also disclosed.

Abstract: A disk shaped cassette for centrifugal fluid separation, particularly blood separation, and a drive system for high speed rotation of the cassette. The cassette is driven at speeds which permit very rapid separation of the blood in times on the order of seven (7) to twenty (20) seconds by an automated control. The cassette is supported by a flexible support coupling and mounting system that permits the entire rotating mass to spin about the center of mass unique to the particular cassette as filled. The cassette typically includes a peripheral collection chamber which may be either annular, lobed or channel shaped. A cassette enabled to hold plural separation tubes is also shown. A gel can be placed in the cassette with a density between the fluid components to be separated and after centrifuging maintains the component separation that centrifuging creates.

Abstract: The invention relates to the control of a system in a blood centrifuge for separating the constituents (fractions) of the whole blood taken from a donor "in vivo" and provided with anticoagulants. A control is provided which as input quantity includes a signal on the flow rate of the full blood and on the output side is connected to the speed setting means for the blood centrifuge. The transfer function of the control is so defined that on a change of the full blood flow the speed of rotation is set so that the volume ratios of the blood fractions to the full blood remain constant. Preferably, the output signal of the control is proportional to the square root of the full blood flow. The control is preferably formed by a microprocessor.

Abstract: An assembly for separating lymphocytes and monocytes from granulocytes in a sample of unseparated whole blood. A water insoluble, thixotropic gel-like substance, which is chemically inert to blood constituents is provided in a container. A fluid capable of altering blood cell densities and diameters is positioned above the surface of the water-insoluble thixotropic gel-like substance. Means for preventing the absorption of water by the thixotropic gel-like substance from the fluid and/or the sample of unseparated whole blood prior to separation are provided to substantially eliminate the influence of water absorption on the cell separation performance characteristics of said thixotropic gel-like substance.

Abstract: A device for separating and isolating heavier components from lighter components of blood mixtures after centrifugal separation, comprising a barrel for holding the blood mixture having slidably positioned therein an inner container for containing the lighter components, a one-way valve responsive to movement of the container within the barrel (a) for controlling the flow of the lighter components into the container, (b) for preventing the lighter components from re-entering the barrel from the container, and (c) for creating a vacuum to assist in drawing the blood mixture to be separated into the barrel, and a filter for filtering the lighter components during their entry into the inner container. The inner container includes an opening for access to the separated and isolated lighter components.

Abstract: A device for separating mononuclear cells from blood includes a collection tube, a first layer of a liquid density gradient material placed in the tube, a second layer of a gel-like substance placed in the tube and situated above the first layer, a porous foam member placed in the tube and situated above and in contact with the second layer of gel-like substance, and a third layer of a Newtonian gel-like substance placed in the tube above the porous foam member. The gel-like substance of the second layer has a lower specific gravity than that of the liquid density gradient material of the first layer so that it floats on top of the first layer. The gel-like substance of the second layer becomes less viscous when heated such that it is absorbed by the porous foam member and forms a hydraulic barrier between the liquid density gradient material of the first layer and a blood sample placed in the collection tube.

Abstract: In a blood serum separator tube for separating blood into a lighter phase and a heavier phase, the thixotropic gel material initially disposed in the container lower end of the tube is protected from contact with any atmosphere contained in the tube, thus preventing separation of silicone oil from the silica-silicone fluid gel material during manufacture or storage. The gel material may be microencapsulated, or contained in a smaller number of larger capsules, or a single capsule. Alternatively, the gel material may be sealed in the tube under a curable elastomeric material, or under a water soluble material which dissolves upon contact with the blood being separated.

Abstract: A system for continuously separating lighter and intermediate density matter, such as plasma rich in platelets from whole blood moves blood through a diverging centrifugation gap between inner and outer walls of a rotor rotating about a central axis within an outer housing. The centrifugation action creates layered flow along an intermediate section. However by crating trailing wakes in the gap between the rotor and housing, localized remixing patterns tending to move in the opposite direction are induced in the layered matter. Platelet rich plasm may then be extracted through adjacent platelet concentrate ports on the inner wall of the rotor. An interior passageway system passes the platelet rich plasma to a platelet concentrate reservoir. Recirculation of blood in the rotor-housing gap between the output and input, and pumping action provided by the diverging centrifugation gap, aid in enhancing throughput and concentration levels.

Abstract: An apparatus for processing and analyzing blood serum includes an input rack for holding test tubes containing whole blood specimens and separator gel, a centrifuge, an optical sensing unit for receiving centrifuged test tubes and generating output signals indicative of whether the centrifuging was successful and, if it was, the boundary position between the separator gel and the blood serum, and a computer connected to receive and analyze the output signals of the optical sensing unit. An aspirator/dispenser needle unit is positioned above the sensing unit and is capable of lowering a needle, under the control of the computer, to puncture the stopper of a test tube and then withdraw blood serum.

Abstract: A device and process for simultaneous separation and transfer utilizes an inverted device which holds a liquid, to be separated, above a collection cup. Preferably the separation is accomplished by filtration. The separation and transfer occur without spillage or contamination of the liquid. The device and process are especially useful for the filtration of liquid samples prior to analysis via gas chromatography or high pressure liquid chromatography.

Abstract: A set for the isolation of cryoprecipitate includes a hollow vessel with a closed first end, a second end and a longitudinal axis, and a nipple extending from and closing the second end, said nipple enclosing a volume of approximately 2 to 5 percent of the volume of the hollow vessel. In one embodiment, the vessel is formed of a semi-rigid material and the first end is closed by a cap containing a micro-porous filter for venting the vessel. In another or further embodiment, the nipple has a tapered tip portion which may be sliced off to permit extrusion of the isolated cryoprecipitate by squeezing the vessel. In a different further embodiment, the nipple has a twist-lockable connector, for attachment to a syringe or to an applicator tip. In yet another embodiment a piston mounts on the cap within the vessel for directly extruding separated cryoprecipitate from the nipple.

Abstract: Closed filtering system for relatively fast and efficient removal of white blood cells from a red blood cell mixture. System comprises at least two blood bags in closed communication with each other via an intermediate filter assembly comprising a housing containing continuous filtering fiber. A preferred housing is tapered and the fiber preferably has a generally Y-shaped cross sectional area and it is adapted to permit substantial removal of white blood cells from a red blood cell mixture with minimal red blood cell hemolysis when the mixture is diluted with preservative solution and passed from one bag to the other at a relatively high flow rate. Filtration is completed within 24 hours, preferably within 6 hours, of whole blood donation.

Abstract: A set for the isolation of cryoprecipitate includes a hollow vessel with first and second ends and a tapered body extending to a narrow sump closing the second end, said sump enclosing a volume of approximately 2 to 5 percent of the volume of the hollow vessel. A port enters the vessel in the tapered body above the sump. In one embodiment, the vessel is formed of a rigid or semi-rigid material and the first end is closed by a cap containing a micro-porous filter for venting the vessel. In another or further embodiment, the sump has a tapered tip portion which may be sliced off to permit extrusion of the isolated cryoprecipitate by squeezing the vessel. In a different embodiment, the vessel contains an inner filter column, or bag with a filter matrix, accessible via an access port. Cellular material placed in the column is frozen, thawed and centrifuged to separate out antigen-free platelet growth factor with the cryoprecipitate.

Abstract: A centrifugal separator system is provided for extracting lighter matter from a liquid suspension. The separator includes a housing having an axis, an inlet port, a lighter matter outlet port and a heavier matter outlet port spaced from the inlet port. A rotatable interior double shell rotor is disposed within the housing. The rotor includes outer and inner shells and defines a centrifugation gap therebetween. The rotor is spaced from the housing by a flow gap. The outer shell includes means providing communication between the centrifugation gap, the inlet port and the heavier outlet port. Means are provided in the double shell rotor for conducting lighter matter from the centrifugation gap to the lighter matter outlet port.

Abstract: A method of removing lipid soluble chemicals from a biological material containing the lipid soluble chemicals comprising bringing the biological material containing the lipid soluble chemicals into contact with an effective amount of an organic alcohol whose solubility in an aqueous solution at the temperature of use is .ltoreq.0.6% and/or a halogenated hydrocarbon having more than one type of halogen, agitating the resultant mixture and achieving a phase separation of the biological material and the organic alcohol and/or halogenated hydrocarbon. The method is particularly useful for producing relatively virus free physiologically acceptable plasma.

Abstract: Isolation of a quantity of blood within a fold of a blood collection bag is prevented during centrifugation by applying a laterally oriented force against the blood collection bag to pressurize it and encourage unfolding of any folds. The laterally oriented force is generated by the apparent weight increase of the blood collection bag during centrifugation.

Abstract: A method of separating blood plasma from particles including blood cells, in the blood, includes the steps:(a) providing a membrane with holes therethrough of a size to pass the particulate, and supplying blood to one side of the membrane,(b) moving the membrane at sufficiently high velocity that shear conditions created in the blood adjacent the membrane cause particles in the blood to move or remain away from the surface of the membrane and from the holes,(c) creating a pressure differential across the membrane sufficient to cause plasma to pass through the holes from said one side of the membrane to the opposite side thereof,(d) the holes being allowed to reduce in cross-sectional area as by protein collection on the membrane at the holes, or by expansion of the membrane material, and the holes maintained sufficiently open, by virtue of their original size, to continue to pass the plama therethrough.

Abstract: A blood separation device includes a collection tube containing a first layer of a thixotropic gel-like substance and a second layer of a newtonian gel-like substance which is positioned below the first layer. The first layer of the thixotropic substance is positioned between the second layer of the newtonian substance and a blood sample received by the tube, prior to centrifuging the tube, and acts as a barrier between the second layer and the blood sample to prevent intermixing of the second layer and the blood sample. The thixotropic gel-like substance of the first layer has a specific gravity which is greater than the newtonian gel-like substance of the second layer whereupon centrifugation of the tube, the first layer moves to a position below the second layer.

Abstract: A positive gauge blood pressure signal is transmitted to a gas column from a pulsatile blood flow stream hydrostatically higher than the pressure transducer. Particularly, a first conduit having a predetermined internal diameter smaller than a second conduit is connected at one end to the blood flow stream. The first conduit extends to a turnaround below the pressure transducer and is connected to the second conduit. The opposite end of the second conduit connects with the pressure transducer. The small internal diameter of the first conduit maintains the meniscus of the blood intact notwithstanding the pulsatile blood stream.

Abstract: A body fluid treating circuit comprising a block for collecting a body fluid from a human body, a body fluid treating block and a block for returning the treated body fluid to the human body, said body fluid treating block comprising a plurality of treating units for treating the body fluid by passing therethrough, the units being arranged in parallel flow relationship, body fluid feed and return lines connected to each of the treating units so as to selectively feed to and return from the units through line switching means.

Abstract: A cell for placing solid matter on a piece of slide glass in such a manner that it is rotated in a centrifugal separator, which is formed integrally by mold forming of synthetic resin thereby to be disposable, and a base plate of a cell portion has an elasticity so as to be easily engaged with a hook provided on a holder in which the base plate is held.

Abstract: This disclosure relates to a blood processing and preservation system. Apparatus of the system comprises a primary collection bag, a blood collection tube connected to the bag and adapted to draw a unit of blood from a person and feed the blood into the bag, and a harness connected to the bag for conducting solutions and blood components into and out of the bag. The harness includes a first tube for removing at least one blood component from the bag while retaining another component, a second tube for moving a cryoprotective solution into and out of the bag, a third tube for conducting a wash solution into and out of the bag, and identification on the bag.