Methods Patents (Class 250/282)
  • Patent number: 10784918
    Abstract: A discrete spectrum (DS) signal transmitter includes a first circuit element comprising a DS signal generator that generates a plurality of DS signals, each DS signal having a different DS frequency, each DS frequency being (a) a harmonic of a fundamental frequency or (b) the fundamental frequency. A second circuit element receives as an input the DS signals and that generates as an output (a) a finite summation of the DS signals or (b) pulses that represent a mathematical equivalent of a summation of an infinite number of the DS signals. An antenna is electrically coupled to an output of the second circuit element. The analog DS signals transmitted by the DS signal transmitter are received by a DS signal receiver that converts the analog DS signals to DS discrete signals and performs a fast Fourier transform of the DS discrete signals.
    Type: Grant
    Filed: September 16, 2019
    Date of Patent: September 22, 2020
    Assignee: Discrete Partners, Inc
    Inventor: James C. Harris
  • Patent number: 10763097
    Abstract: A mass spectrometer for analyzing fragment ions includes an ion source, an ion mobility spectrometer, and a system of electrodes. The ion source is configured to produce fragment ions. The ion mobility spectrometer is configured to receive fragment ions produced by the ion source and to separate at least a fraction of the received fragment ions according to their ion mobility into mobility-separated fragment ions. The system of electrodes is configured to receive DC electrical potentials to transfer at least a fraction of the mobility-separated fragment ions to a mass analyzer for generating a mass-to-charge (m/z) spectrum exhibiting reduced spectral complexity.
    Type: Grant
    Filed: May 13, 2019
    Date of Patent: September 1, 2020
    Assignee: FASMATECH SCIENCE & TECHNOLOGY LTD.
    Inventor: Dimitris Papanastasiou
  • Patent number: 10763093
    Abstract: Neutral particles are blocked by a deflector provided upstream of a detector. A controller changes a reference potential V2 of the deflector in connection with a change of a reference potential V1 of a collision cell such that a potential difference ?V between the reference potential V1 and the reference potential V2 is constant. The change of the reference potential V2 is executed during a period in which an ion pulse does not pass the deflector.
    Type: Grant
    Filed: March 8, 2019
    Date of Patent: September 1, 2020
    Assignee: JEOL Ltd.
    Inventor: Junkei Kou
  • Patent number: 10755906
    Abstract: A miniature mass spectrometer is disclosed comprising an atmospheric pressure ionization source and a first vacuum chamber having an atmospheric pressure sampling orifice or capillary, a second vacuum chamber located downstream of the first vacuum chamber and a third vacuum chamber located downstream of the second vacuum chamber. An ion detector is located in the third vacuum chamber. A first RF ion guide is located within the first vacuum chamber and a second RF ion guide is located within the second vacuum chamber. The ion path length from the atmospheric pressure sampling orifice or capillary to an ion detecting surface of the ion detector is ?400 mm. The mass spectrometer further comprises a tandem quadrupole mass analyzer, a 3D ion trap mass analyzer, a 2D or linear ion trap mass analyzer, a Time of Flight mass analyzer, a quadrupole-Time of Flight mass analyzer or an electrostatic mass analyzer arranged in the third vacuum chamber.
    Type: Grant
    Filed: January 2, 2019
    Date of Patent: August 25, 2020
    Assignee: Micromass UK Limited
    Inventors: David Gordon, Daniel James Kenny
  • Patent number: 10753863
    Abstract: Spectroscopic chemical analysis methods and apparatus are disclosed which employ deep ultraviolet (e.g. in the 200 nm to 300 nm spectral range) electron beam pumped wide bandgap semiconductor lasers, incoherent wide bandgap semiconductor light emitting devices, and hollow cathode metal ion lasers to perform non-contact, non-invasive detection of unknown chemical analytes. These deep ultraviolet sources enable dramatic size, weight and power consumption reductions of chemical analysis instruments. In some embodiments, Raman spectroscopic detection methods and apparatus use ultra-narrow-band angle tuning filters, acousto-optic tuning filters, and temperature tuned filters to enable ultra-miniature analyzers for chemical identification. In some embodiments Raman analysis is conducted along with photoluminescence spectroscopy (i.e. fluorescence and/or phosphorescence spectroscopy) to provide high levels of sensitivity and specificity in the same instrument.
    Type: Grant
    Filed: November 19, 2019
    Date of Patent: August 25, 2020
    Assignee: Photon Systems, Inc.
    Inventors: William F. Hug, Ray D. Reid, Rohit Bhartia, Arthur L. Lane
  • Patent number: 10752733
    Abstract: A binder for an electrochemical element, comprising: a polymer having a carboxyl group and/or a salt thereof, a polymer having an amide group and/or an amide bond; or a polymer having a carboxyl group and/or a salt thereof and an amide group and/or an amide bond.
    Type: Grant
    Filed: April 6, 2017
    Date of Patent: August 25, 2020
    Assignee: IDEMITSU KOSAN CO.,LTD.
    Inventor: Yu Ishihara
  • Patent number: 10746709
    Abstract: A chromatograph device capable of correct measurement even if the retention times of target components change. This chromatograph device is provided with: a storage unit for storing measurement parameters for a plurality of target components, measurement conditions including measurement parameter switching times for each of two target components eluted in succession, and formulas for determining the switching times from predetermined retention times for the target components; a measurement data accumulation unit; a preceding-measurement-data determination unit for determining, at the time of sample measurement, whether there is preceding measurement data for the same column type, mobile phase type, and flow velocity; and a measurement execution unit for carrying out measurement on the basis of the measurement conditions if there is no preceding measurement data.
    Type: Grant
    Filed: July 6, 2016
    Date of Patent: August 18, 2020
    Assignee: Shimadzu Corporation
    Inventor: Daisuke Nakayama
  • Patent number: 10748756
    Abstract: A system comprises an electrostatic trapping mass analyzer and an information processor configured to receive a transient signal from the electrostatic trapping mass analyzer at a maximum resolution, the information processor comprising instructions operable to: partition the transient signal into segments and, while a quality metric is either less than a pre-determined minimum threshold or greater than a pre-determined maximum threshold value, to perform the steps of: (i) defining a test transient as being equal to either a first one of the segments or a previously defined transient with an appended signal segment; (ii) generating a spectrum of component frequencies by calculating a mathematical transform of the test transient; and (iii) determining the quality metric from the spectrum of component frequencies; and set an instrumental resolution to be employed for subsequent mass spectral data acquisitions in accordance with a length of the most-recently-defined test transient.
    Type: Grant
    Filed: February 5, 2020
    Date of Patent: August 18, 2020
    Assignee: Thermo Finnigan LLC
    Inventor: Thomas D. McClure
  • Patent number: 10741373
    Abstract: The invention relates to a method for operating an ion gate having a first, a second, and a third electrode which are arranged one after the other in an intended drifting direction of ions to be influenced by the ion gate, in such a way that the second electrode is arranged after the first electrode and the third electrode is arranged after the second electrode in the drift direction. The ion gate can be switched between a closed state, in which ions cannot drift through the ion gate in the intended drifting direction, and an open state, in which ions can drift through the ion gate in the intended drifting direction. This is accomplished by applying potentials that alternate over time to one or more of the electrodes. In a switching cycle of the ion gate, which comprises the open state and the closed state of the ion gate, two different closed states of the ion gate are produced. In a first closed state, the ion gate is closed by applying a first potential between the second and third electrodes.
    Type: Grant
    Filed: August 9, 2019
    Date of Patent: August 11, 2020
    Assignee: GOTTFRIED WILHELM LEIBNIZ UNIVERSITÄT HANNOVER
    Inventors: Stefan Zimmermann, Ansgar Kirk
  • Patent number: 10741379
    Abstract: The invention provides a mass spectrometer, an ion optical device, and a method for ion manipulation in a mass spectrometer. The mass spectrometer includes a mass analyzer; and an ion guiding device, including two electrode arrays positioned in parallel with each other, each electrode array including at least two ring electrodes concentrically disposed or at least three linear electrode assemblies having a radial distribution; and a power supply means, configured to apply a voltage on at least a part of the ring electrodes, to form a radio-frequency electric field and a DC electric field. By means of the radio-frequency electric field and the DC electric field, ions are allowed to be stored in a region between the two arrays, and controlled to be sequentially released along a radial direction according to a preset mass-to-charge ratio requirement, then exit the ion guiding device and enter the mass analyzer for mass analysis.
    Type: Grant
    Filed: February 8, 2017
    Date of Patent: August 11, 2020
    Assignee: SHIMADZU CORPORATION
    Inventors: Xiaoqiang Zhang, Wenjian Sun
  • Patent number: 10739320
    Abstract: For a sample containing a target component, a product-ion scan measurement in which the m/z value of a known ion originating from the compound is designated as a precursor ion is performed in a measurement unit (1) to acquire profile spectrum data. A peak detector (22) in a data processing unit (2A) detects peaks on the profile spectrum. For each detected peak, a product-ion m/z-value acquirer (23) acquires an m/z value corresponding to the maximum intensity as the m/z value of a product ion. A pseudo MRM measurement data extractor (24) adopts the m/z value of the precursor ion and that of the product ion as an MRM transition, extracts the maximum intensity of the peak originating from the product ion as the signal intensity value on that MRM transition, and stores these data as pseudo MRM measurement data in a memory section (25).
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: August 11, 2020
    Assignee: SHIMADZU CORPORATION
    Inventors: Hideki Yamamoto, Yoshikatsu Umemura
  • Patent number: 10741375
    Abstract: An ion carousel includes a first surface and a second surface adjacent to the first surface. The first and the second surfaces define an ion confinement volume. The second surface including a first inner array of electrodes arranged along a first path and configured to receive, at a first location on the first path, a first ion packet. The first inner array of electrodes are configured to generate a plurality of potential wells that include a first potential well and a second potential well. The first ion packet includes a first sub-packet of ions having a first mobility and a second sub-packet of ions having a second mobility, and the second ion packet includes a third sub-packet of ions having the first mobility and a fourth sub-packet of ions having the second mobility.
    Type: Grant
    Filed: May 14, 2019
    Date of Patent: August 11, 2020
    Assignee: MOBILion Systems, Inc.
    Inventors: Ahmed Hamid, Gordon A. Anderson, Daniel DeBord
  • Patent number: 10734208
    Abstract: An MS2 analysis for one precursor ion is performed to collect data on each micro area within a measurement target area (S1). A plurality of product ions are extracted based on those data (S2), and a mass spectrometric (MS) imaging graphic is created for each m/z of the product ion (S3). Hierarchical cluster analysis is performed on the created MS imaging graphics to group the product ions based on the similarity of the graphics (S4). Product ions having similar distributions are sorted into the same group. Such a group of ions can be considered to have originated from the same compound. Accordingly, the intensity information of a plurality of product ions is totaled in each group and for each micro area (S5), and an MS imaging graphic is created based on the totaled intensity information (S6). Even if there are a plurality of compounds overlapping the precursor ion, the influence of the overlapping can be eliminated through those steps.
    Type: Grant
    Filed: May 10, 2016
    Date of Patent: August 4, 2020
    Assignee: SHIMADZU CORPORATION
    Inventor: Kengo Takeshita
  • Patent number: 10734213
    Abstract: An intermittent sample inlet device and methods for use of the intermittent sample inlet device are described that include an orifice plate with an entrance orifice and a rotating skimmer with a skimmer orifice, where the rotating skimmer is disposed between the orifice plate and a vacuum chamber wall. The rotating skimmer rotates so that the skimmer orifice intermittently aligns with the entrance orifice and allows an ion sample to pass through to a mass analyzer.
    Type: Grant
    Filed: July 31, 2014
    Date of Patent: August 4, 2020
    Assignee: Smiths Detection Inc.
    Inventor: Vadym Berkout
  • Patent number: 10732159
    Abstract: The invention generally relates to ion generation using modified wetted porous materials. In certain aspects, the invention generally relates to systems and methods for ion generation using a wetted porous substrate that substantially prevents diffusion of sample into the substrate. In other aspects, the invention generally relate to ion generation using a wetted porous material and a drying agent. In other aspects, the invention generally relates to ion generation using a modified wetted porous substrate in which at least a portion of the porous substrate includes a material that modifies an interaction between a sample and the substrate.
    Type: Grant
    Filed: August 21, 2018
    Date of Patent: August 4, 2020
    Assignee: Purdue Research Foundation
    Inventors: Robert Graham Cooks, Zheng Ouyang
  • Patent number: 10725013
    Abstract: A system, method and computer program product are provided for calculating one or more indicative properties including one or more of the cetane number, octane number, pour point, cloud point and aniline point of oil fractions, from the density and Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) of a sample of an oil sample.
    Type: Grant
    Filed: June 30, 2017
    Date of Patent: July 28, 2020
    Assignee: SAUDI ARABIAN OIL COMPANY
    Inventors: Omer Refa Koseoglu, Adnan Al-Hajji, Hendrik Muller, Hanadi H. Jawad
  • Patent number: 10727036
    Abstract: A method of mass spectrometry is disclosed comprising: performing a plurality of cycles of operation during a single experimental run, wherein each cycle comprises: mass selectively transmitting precursor ions of a single mass, or range of masses, through or out of a mass separator or mass filter at any given time, wherein the mass separator or mass filter is operated such that the single mass or range of masses transmitted therefrom is varied with time; operating the mass separator or filter in a wideband mode between at least some of said plurality of cycles, wherein in each wideband mode the mass separator or filter transmits ions in a non-mass resolving manner; and mass analysing ions.
    Type: Grant
    Filed: April 13, 2017
    Date of Patent: July 28, 2020
    Assignee: Micromass UK Limited
    Inventors: Keith Richardson, Jason Lee Wildgoose
  • Patent number: 10727041
    Abstract: The invention generally relates to systems and methods for separating ions at about or above atmospheric pressure. In certain embodiments, the invention provides systems that include an ionization source that generates ions and an ion trap. The ion trap is maintained at about or above atmospheric pressure and includes a plurality of electrodes and at least one inlet configured to receive a gas flow and at least one outlet. The system is configured such that a combination of a gas flow and one or more electric fields produced by the electrodes separates the ions based on mass-to-charge ratio and sends the separated ions through the at least one outlet of the ion trap.
    Type: Grant
    Filed: January 27, 2017
    Date of Patent: July 28, 2020
    Assignee: Purdue Research Foundation
    Inventors: Robert Graham Cooks, Dalton Snyder, Amar S. Basu, Wen-Ping Peng
  • Patent number: 10727040
    Abstract: A mass spectrometer is disclosed comprising an ion mobility separation device for separating ions according to their ion mobility, a first quadrupole mass filter downstream of the ion mobility separation device, a control system arranged and adapted to scan and/or step the set mass of the first quadrupole mass filter a plurality of times over a first mass to charge ratio range of <±2 amu during the elution time of an ion mobility peak from the ion mobility separation device, and an analyser or ion detector downstream of the first quadrupole mass filter arranged and adapted to analyse or detect ions so as to acquire multi-dimensional ion mobility-mass to charge ratio data.
    Type: Grant
    Filed: September 27, 2018
    Date of Patent: July 28, 2020
    Assignee: Micromass UK Limited
    Inventors: Martin Raymond Green, Keith Richardson, Jason Lee Wildgoose
  • Patent number: 10720315
    Abstract: An ion transfer device that transfers ions from at least one ion inlet to at least one ion outlet. The ion transfer device includes an enclosure configured to maintain reduced pressure, and a plurality of electrodes disposed at least in part inside the enclosure such that the ion transfer device is configured to be flexible or re-configurable.
    Type: Grant
    Filed: October 3, 2018
    Date of Patent: July 21, 2020
    Assignee: Trace Matters Scientific LLC
    Inventor: Mazdak Taghioskoui
  • Patent number: 10714210
    Abstract: A method of analysing a sample mass spectrum comprises comparing a sample mass spectrum of a sample with each reference mass spectrum of plural reference mass spectra. A similarity index is assigned to each reference mass spectrum of the plural reference mass spectra based on similarity between the sample mass spectrum and the reference mass spectrum. For each group of one or more groups of the plural reference mass spectra, the similarity indexes for the reference mass spectra belonging to the group are combined so as to provide a group index for the group at a first level of a hierarchy of sample characteristics. The reference mass spectra belonging to each group are mass spectra of reference samples that have a particular characteristic in common. The method provides a way to categorise a sample as belonging to a particular group of reference samples.
    Type: Grant
    Filed: May 27, 2016
    Date of Patent: July 14, 2020
    Assignee: Micromass UK Limited
    Inventors: Keith George Richardson, Steven Derek Pringle
  • Patent number: 10699889
    Abstract: Disclosed herein is an ion guide comprising a plurality of axially stacked plates, wherein at least some or all of said plates comprise: a first electrically conductive portion; and a second electrically conductive portion, wherein the second electrically conductive portion is electrically isolated from the first electrically conductive portion, the first and second electrically conductive portions being shaped and arranged relative to each other so as to define an opening through which ions are axially transmitted in use; wherein, in use, a first AC or RF voltage is applied to the first electrically conductive portion and a second AC or RF voltage is applied to the second electrically conductive portion in order to confine ions radially within said opening. The first and second electrically conductive portions (1, 2) may be separately formed and interleaved within the ion guide to define the plates.
    Type: Grant
    Filed: May 15, 2017
    Date of Patent: June 30, 2020
    Assignee: MICROMASS UK LIMITED
    Inventors: Stephen Patrick Slater, John Iveson, Oliver John Malpas, Bharat Chande, David Gordon
  • Patent number: 10697980
    Abstract: Methods are provided for detecting the amount of one or more CAH panel analytes (i.e., pregnenolone, 17-OH pregnenolone, progesterone, 17-OH progesterone, dehydroepiandrosterone (DHEA), androstenedione, testosterone, deoxycorticosterone, 11-deoxycortisol, and cortisol) in a sample by mass spectrometry. The methods generally involve ionizing one or more CAH panel analytes in a sample and quantifying the generated ions to determine the amount of one or more CAH panel analytes in the sample. In methods where amounts of multiple CAH panel analytes are detected, the amounts of multiple analytes are detected in the same sample injection.
    Type: Grant
    Filed: September 30, 2019
    Date of Patent: June 30, 2020
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Amit Ghoshal, Nigel J. Clarke, Mildred M. Goldman
  • Patent number: 10697932
    Abstract: A method of mass spectrometry is disclosed comprising selecting a modification of interest that may modify the mass to charge ratios of precursor ions of interest when said precursor ions are subjected to a fragmentation or reaction condition for producing product ions. The method then filters the product ions (or product ion data) such that only a subset of the product ions are transmitted and detected (or a subset of the data remains) and so as to exclude product ions (or product ion data) that could not have possibly resulted from the modification of interest. This significantly simplifies the product ion data, enabling the product ions to be identified or compared to precursor ion spectra more efficiently.
    Type: Grant
    Filed: November 11, 2014
    Date of Patent: June 30, 2020
    Assignee: MICROMASS UK LIMITED
    Inventor: Jason Lee Wildgoose
  • Patent number: 10685827
    Abstract: A quadrupole ion trap apparatus includes a main electrode, a first end-cap electrode, a second end-cap electrode, and a phase-controlled waveform synthesizer. The phase-controlled waveform synthesizer generates a main RE waveform for the main electrode. The main RE waveform includes a plurality of sinuous waveform segments each of which is a part of a sine wave, and a plurality of phase conjunction segments each of which is non-sinuous. Each of the sinuous waveform segments is bridged to another sinuous waveform segment via one of the phase conjunction segments, so as to perform ordering of micro motions of sample ions trapped by the electrodes.
    Type: Grant
    Filed: May 8, 2018
    Date of Patent: June 16, 2020
    Assignee: ACROMASS TECHNOLOGIES, INC.
    Inventors: Chun-Yen Cheng, Yao-Hsin Tseng, Szu-Wei Chou, Yi-Kun Lee, Shih-Chieh Yang, Hung-Liang Hsieh
  • Patent number: 10684255
    Abstract: A method of Fourier Transform ion mobility mass spectrometry is disclosed comprising determining the presence of different types of ions by determining that the different types of ions have different combinations of ion mobility and mass to charge ratio.
    Type: Grant
    Filed: March 23, 2016
    Date of Patent: June 16, 2020
    Assignee: Micromass UK Limited
    Inventors: Martin Raymond Green, Keith George Richardson
  • Patent number: 10677922
    Abstract: A time-of-flight (TOF) sensor device is provided with features for correcting distance measurement offset errors caused by such factors as temperature, dynamic reflectivity ranges of objects in the viewing space, or other factors. In various embodiments, the TOF sensor device generates corrected distance values based on comparison of two different distance values measured for an object by two different measurement techniques, including but not limited to phase shift measurement, pulsed TOF measurement, distance measurement based on the focal length of the TOF sensor's lens, and comparison of distance variations with light intensity variations. In addition, some embodiments of the TOF sensor device perform self-calibration using internal waveguides or parasitic reflections as distance references.
    Type: Grant
    Filed: October 10, 2017
    Date of Patent: June 9, 2020
    Assignee: Rockwell Automotive Safety AG
    Inventors: Carl Meinherz, Martin Hardegger, Manfred Stein, Danilo Dorizzi
  • Patent number: 10672600
    Abstract: Provided are methods for determining the amount of reverse T3 in a sample using mass spectrometry. The methods generally involve ionizing reverse T3 in a sample and detecting and quantifying the amount of the ion to determine the amount of reverse T3 in the sample.
    Type: Grant
    Filed: September 17, 2018
    Date of Patent: June 2, 2020
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: J. Fred Banks, Peter P. Chou, Noriya M. Matt
  • Patent number: 10663428
    Abstract: A system for analyzing a sample includes a source; a mobility separator configured to separate ions based on a mobility in a gas; a plurality of ion channels; and a mass analyzer. The mobility separator includes a two-dimensional grid of electrodes spanning a passage between first and second walls. The first and second walls include an inlet aperture and a plurality of exit apertures, respectively. The two-dimensional grid of electrodes configured to generate an electric field within the passage. The plurality of ion channels arranged adjacent to the plurality of exit apertures. Movement of ions between the inlet aperture and the plurality of exit apertures are governed by the electric field and a gas flow through the passage between to the first and second walls such that the ions are sorted and directed to different channels based on their respective mobility.
    Type: Grant
    Filed: June 29, 2018
    Date of Patent: May 26, 2020
    Assignee: Thermo Finnigan LLC
    Inventor: Mikhail V. Ugarov
  • Patent number: 10665438
    Abstract: An elemental mass spectrometer uses a mass filter to select ions from ions received from an ion source and transmit the selected ions. A reaction or collision cell receives the transmitted ions and reacts or collides these with a gas to provide product ions thereby. A mass analyzer receives the product ions, analyzes them and provides at least one output based on detection of the analyzed ions. The elemental mass spectrometer is operated to provide a first output from the mass analyzer measuring ions within a first analysis range of mass-to-charge ratios including a desired mass-to-charge ratio, M, to provide a second output from the mass analyzer measuring ions within a second analysis range of mass-to-charge ratios including a mass-to-charge ratio at least 0.95 atomic mass units lower than the desired mass-to-charge ratio, (M?i), i?0.95 and to correct the first output on the basis of the second output.
    Type: Grant
    Filed: September 9, 2016
    Date of Patent: May 26, 2020
    Assignee: Thermo Fisher Scientific (Bremen) GMBH
    Inventors: Lothar Rottmann, Alexander Makarov, Hans-Juergen Schlueter, Christoph Wehe
  • Patent number: 10656127
    Abstract: A data independent acquisition method of mass spectrometry for analysing a sample as it elutes from a chromatography system is disclosed. The method comprises the steps of: ionising the sample to produce precursor ions, selecting a precursor mass range for the sample to be analysed, performing a plurality of MS1 scans and performing at most two sets of MS2 scans. Each of the MS1 scans uses a mass analyser operated at a first, relatively higher resolution, for identification and/or quantitation of the sample in the MS1 domain across the precursor mass range. The set of MS2 scans comprises performing MS2 scans of fragmented mass range segments performed with the mass analyser, operated at a second, relatively lower resolution. In the method, the MS1 scans are interleaved throughout the performing of the set of MS2 scans such that the MS1 scans provide a mass chromatogram of the sample.
    Type: Grant
    Filed: January 31, 2018
    Date of Patent: May 19, 2020
    Assignee: Thermo Fisher Scientific (Bremen) GmbH
    Inventor: Yue Xuan
  • Patent number: 10658165
    Abstract: A measure of abundance is determined for an element or element combination within a sample, the element or element combination having at least one isotopic variant. An isotopic mass spectral pattern is identified for the element or element combination that indicates an expected abundance and expected mass-to-charge ratio difference for each isotopic variant. These are identified relative to the respective abundance and mass-to-charge ratio of a principal isotope. The isotopic mass spectral pattern is compared with mass spectral data from a molecular mass analysis of the sample to identify peak groups, each matching the isotopic mass spectral pattern. A measure of abundance is determined for the element or element combination as a function of the intensity measurement of one or more peaks from each of the identified peak groups.
    Type: Grant
    Filed: June 6, 2014
    Date of Patent: May 19, 2020
    Assignee: Thermo Fisher Scientific (Bremen) GmbH
    Inventors: Hans Pfaff, Timothy J. Stratton
  • Patent number: 10643820
    Abstract: A multi-beam apparatus for observing a sample with high resolution and high throughput is proposed. In the apparatus, a source-conversion unit changes a single electron source into a virtual multi-source array, a primary projection imaging system projects the array to form plural probe spots on the sample, and a condenser lens adjusts the currents of the plural probe spots. In the source-conversion unit, the image-forming means is on the upstream of the beamlet-limit means, and thereby generating less scattered electrons. The image-forming means not only forms the virtual multi-source array, but also compensates the off-axis aberrations of the plurality of probe spots.
    Type: Grant
    Filed: April 29, 2019
    Date of Patent: May 5, 2020
    Assignee: HERMES MICROVISION INC.
    Inventors: Weiming Ren, Shuai Li, Xuedong Liu, Zhongwei Chen
  • Patent number: 10643396
    Abstract: Methods and software assisting a user in working with images of histological sections to increase the user's productivity and decrease the need for extensive expertise in anatomy. In some embodiments, the methods include methods of assisting a user in creating a 3D volume image of a tissue block from a series of images of histological sections taken from the tissue block. In some embodiments, the methods include methods of automatedly registering a live-view or stored histological section image to a tissue block atlas. In some embodiments, the methods include methods of annotating a histological section image with information from a tissue block atlas based on user input(s) associated with the tissue block atlas. In some embodiments, the methods include methods of automatedly controlling operation of an imaging modality, such as an optical microscope, based on user input(s) associated with a tissue block atlas. These and other methods may be embodied in various configurations of software.
    Type: Grant
    Filed: November 13, 2017
    Date of Patent: May 5, 2020
    Assignee: Microbrightfield, Inc.
    Inventors: Brian S. Eastwood, Susan Tappan, Nathan J. O'Connor
  • Patent number: 10628751
    Abstract: A method of processing sensor logs is described. The method includes accessing a first sensor log and a corresponding first reference log. Each of the first sensor log and the first reference log includes a series of measured values of a parameter according to a first time series. The method also includes accessing a second sensor log and a corresponding second reference log. Each of the second sensor log and the second reference log includes a series of measured values of a parameter according to a second time series. The method also includes dynamically time warping the first reference log and/or and second reference log by a first transformation between the first time series and a common time-frame and/or a second transformation between the second time series and the common time-frame. The method also includes generating first and second warped sensor logs by applying the or each transformation to the corresponding ones of the first and second sensor logs.
    Type: Grant
    Filed: July 16, 2019
    Date of Patent: April 21, 2020
    Assignee: Palantir Technologies Inc.
    Inventors: Ezra Spiro, Andre Frederico Cavalheiro Menck, Peter Maag, Thomas Powell
  • Patent number: 10626141
    Abstract: The present invention provides a method for specifically cleaving a C?-C bond of a peptide backbone and/or a side chain of a protein and a peptide, and a method for determining amino acid sequences of protein and peptide. A method for specifically cleaving a C?-C bond of a peptide backbone and/or a side chain bond of a protein or a peptide, comprising irradiating a protein or a peptide with laser light in the presence of at least one hydroxynitrobenzoic acid selected from the group consisting of 3-hydroxy-2-nitrobenzoic acid, 4-hydroxy-3-nitrobenzoic acid, 5-hydroxy-2-nitrobenzoic acid, 3-hydroxy-5-nitrobenzoic acid, and 4-hydroxy-2-nitrobenzoic acid. A method for determining an amino acid sequence of a protein or a peptide, comprising irradiating a protein or a peptide with laser light in the presence of the above specific hydroxynitrobenzoic acid to specifically cleave a C?-C bond of a peptide backbone and/or a side chain bond, and analyzing generated fragment ions by mass spectrometry.
    Type: Grant
    Filed: May 12, 2017
    Date of Patent: April 21, 2020
    Assignees: SHIMADZU CORPORATION, NATIONAL UNIVERSITY OF CORPORATION HIROSHIMA UNIVERSITY
    Inventors: Shunsuke Izumi, Koichi Tanaka, Yuko Fukuyama
  • Patent number: 10622199
    Abstract: The invention relates to methods and devices for analysis of samples using laser ablation imaging mass cytometry and mass spectrometry. The invention provides methods and devices in which individual ablation plumes are distinctively captured and rapidly transferred to the ionization system, followed by analysis by mass spectrometry. A transfer conduit can be used to convey ablation plumes to an ionization system. The transfer conduit can include an asymmetric cone. The transfer conduit can be tapered. A flow sacrificing system can be adapted to divert a part of the sheath flow out a sacrificial outlet while the core of the sheath flow containing ablation plumes enters the ionization system.
    Type: Grant
    Filed: August 2, 2017
    Date of Patent: April 14, 2020
    Assignee: FLUIDIGM CANADA INC.
    Inventor: Alexander V. Loboda
  • Patent number: 10622200
    Abstract: Certain configurations of ionization sources are described. In some examples, an ionization source comprises an ionization block, an electron source, an electron collector, an ion repeller and at least one electrode configured to provide an electric field when a voltage is provided to the at least one electrode. Systems and methods using the ionization source are also described.
    Type: Grant
    Filed: May 18, 2018
    Date of Patent: April 14, 2020
    Assignee: PerkinElmer Health Sciences Canada, Inc.
    Inventors: Anna Kornilova, Gholamreza Javahery, Lisa Cousins
  • Patent number: 10615014
    Abstract: A method of mass spectrometry is disclosed comprising comparing mass spectral data so as to identify a precursor ion in the mass spectral data that has a predetermined mass difference to a product ion in the mass spectral data; and determining whether said precursor ion is a precursor ion of interest by comparing the ion signal profile for the precursor ion with the ion signal profile for the product ion. If the profiles match then the precursor ion is determined to be an ion of interest. When a precursor ion is determined to be an ion of interest, the precursor ion is isolated from other precursor ions, fragmented or reacted so as to produce product ions, and the product ions are analysed so as to obtain product ion data that can be used to identify the precursor ion.
    Type: Grant
    Filed: November 11, 2014
    Date of Patent: April 7, 2020
    Assignee: Micromass UK Limited
    Inventors: Keith Richardson, Jason Lee Wildgoose
  • Patent number: 10613054
    Abstract: An analytical device for analyzing ions is provided comprising a separator 2 for separating ions according to a physico-chemical property and an interface 3 comprising one or more ion guides. A quadrupole rod set mass filter 4 is arranged downstream of the interface 3. A control system is arranged and adapted: (i) to transmit a first group of ions which emerges from the separator 2 through the interface 3 with a first transit time t1; and (ii) to transmit a second group of ions which subsequently emerges from the separator 2 through the interface 3 with a second different transit time t2.
    Type: Grant
    Filed: October 15, 2018
    Date of Patent: April 7, 2020
    Assignee: Micromass UK Limited
    Inventors: Kevin Giles, Steven Derek Pringle, Jason Lee Wildgoose
  • Patent number: 10615024
    Abstract: An electrostatic analyzer (ESA) includes a coaxial structure having an outer conductive cylinder, an inner conductive cylinder, and one or more pathways disposed therebetween and extending from a first end to a second end of the coaxial structure. The outer conductive cylinder and the inner conductive cylinder may each be structurally configured to receive a bias voltage for creation of a predetermined electric field therebetween that allows for passage of charged particles with a predetermined energy/charge band along a helical path through the coaxial structure. The ESA may further include an entrance filter on the first end of the coaxial structure that defines a plurality of openings aligned at a predetermined angle thereby limiting one or more fields of view between a pathway through the ESA and the external environment to filter particles, by their trajectory, from entering the pathway for analysis by the ESA.
    Type: Grant
    Filed: December 18, 2018
    Date of Patent: April 7, 2020
    Assignee: THE GOVERNMENT OF THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE AIR FORCE
    Inventors: Patrick Roddy, David Barton, Joseph Coombs, John Ballenthin, John McGarity, Scott Kratochvil
  • Patent number: 10613062
    Abstract: A signal intensity based on a total ion current signal or the like is calculated from mass spectra obtained by a normal mode of mass spectrometric analysis. When the signal intensity has exceeded an intensity threshold, the beginning time T0 of a chromatogram peak is estimated from that signal intensity as well as one or more previous signal intensities. An MS2 execution permission-beginning time Ts is calculated by adding, to the beginning time T0, a delay time Tdelay determined front a half-value width of the peak estimated according to an LC separation condition. At or after the point in time where the actual time passes Ts, a peak which satisfies a precursor-ion selection condition is selected on a mass spectrum obtained by the mass spectrometric analysis. Then, an MS2 analysis with the m/z of the selected peak as the target is immediately performed to obtain an MS2 spectrum.
    Type: Grant
    Filed: October 15, 2015
    Date of Patent: April 7, 2020
    Assignee: SHIMADZU CORPORATION
    Inventors: Yoshitake Yamamoto, Hideki Yamamoto
  • Patent number: 10615015
    Abstract: Methods of identification of at least one most likely elemental composition of at least one species of molecules contained in a sample and/or originated from a sample by at least one ionisation process are provided. The method includes measuring a mass spectrum of the sample and may include reducing the measured mass spectrum to a neutral mass spectrum. The method further includes determining for a peak of interest a set of candidate species of molecules which have an expected peak with a peak position within a peak position tolerance range in the corresponding measured mass spectrum or neutral mass spectrum. An identification mass spectrum is identified for each candidate species and a range of peak positions is determined of all peaks of the identification mass spectrum. Two subscores of candidate species are determined by comparing the identification spectra with the measured or neutral mass spectrum and final scores are calculated from the subscores.
    Type: Grant
    Filed: February 23, 2017
    Date of Patent: April 7, 2020
    Assignee: Thermo Fisher Scientific (Bremen) GMBH
    Inventors: Martin Strohalm, Hans Pfaff, Evgenia Razumovski
  • Patent number: 10615022
    Abstract: The invention relates to the operation of an energy-focusing and solid-angle-focusing reflector for time-of-flight mass spectrometers with pulsed ion acceleration into a flight tube, e.g. from an ion source with ionization by matrix-assisted laser desorption (MALDI). The objective of the invention is to generate high mass resolution in wide mass ranges up to high masses above eight kilodaltons by varying at least one operating voltage on one of the diaphragms of the reflector which can be varied according to a suitable time function during the spectrum acquisition. It may also be advantageous to adapt the operation of the accelerating voltages in the starting region of the ions accordingly. These measures make it possible to achieve a mass resolution much higher than R=100,000 in a wide mass range extending up to and above eight kilodaltons.
    Type: Grant
    Filed: September 17, 2018
    Date of Patent: April 7, 2020
    Inventors: Sebastian Böhm, Andreas Haase
  • Patent number: 10607827
    Abstract: An ionising apparatus for ionising a sample of gaseous fluid. The ionising apparatus comprises an ioniser configured to provide reactant ions; an ion modifier configured to modify the reactant ions, and a reaction region arranged to receive the modified reactant ions and a sample and to combine the sample with the modified reactant ions to ionise the sample for analysis by a detector configured to identify a substance of interest in the sample.
    Type: Grant
    Filed: March 15, 2018
    Date of Patent: March 31, 2020
    Assignee: SMITHS DETECTION-WATFORD LIMITED
    Inventors: Stephen Taylor, Jonathan Atkinson
  • Patent number: 10600631
    Abstract: An ion trap having a segmented electrode structure having a plurality of segments consecutively positioned along an axis, wherein each segment of the segmented electrode structure includes a plurality of electrodes arranged around the axis. A first voltage supply is configured to operate in a radially confining mode in which at least some electrodes belonging to each segment are supplied with at least one AC voltage waveform so as to provide a confining electric field for radially confining ions within the segment. A second voltage supply is configured to operate in a trapping mode in which at least some of the electrodes belonging to the segments are supplied with different DC voltages so as to provide a trapping electric field that has an axially varying profile for urging ions towards and trapping ions in a target segment of the plurality of segments. A first chamber is configured to receive ions from an ion source, wherein a first subset of the segments are located within the first chamber.
    Type: Grant
    Filed: August 31, 2018
    Date of Patent: March 24, 2020
    Assignee: SHIMADZU CORPORATION
    Inventors: Roger Giles, Matthew Clive Gill
  • Patent number: 10598639
    Abstract: When performing an analysis of the difference between a specific sample group and a nonspecific sample group, a principle component analysis processing unit (33) performs principle component analysis on a collection of a plurality of mass spectrums created from data obtained for a single specific sample, and a characteristic spectrum acquisition unit (34) acquires a characteristic spectrum for each of a plurality of principle components using factor loadings. A spectrum similarity calculation unit (35) calculates the similarities between all mass spectrums and the characteristic spectrum for each sample, and obtains a representative value for the same. The similarity representative value for each sample is obtained for all the characteristic spectrums.
    Type: Grant
    Filed: January 26, 2015
    Date of Patent: March 24, 2020
    Assignee: Shimadzu Corporation
    Inventor: Yuriko Nakakimura
  • Patent number: 10598641
    Abstract: A method of assessing the linearity and dynamic range of a mass spectrometer is described comprising analyzing two or more test standards containing positional isomers, for example, using a series of N isomeric analytes with or without an internal standard where N is an integer from 2 to 6 inclusive. The concentrations of the N isomeric analytes may or may not be interleaved between two or more standards. The method allows for repeated instrument characterization without having many of the problems associated with using only a single standard such as octafluoronaphthalene (OFN), for example, persistence, and it may lessen sample carryover issues between adjacent samples. Methods are described using positional isomers of dibromodifluorobenzene in various configurations that show an improved means for qualitative and quantitative analysis of one or more analytes by GC-MS.
    Type: Grant
    Filed: December 17, 2018
    Date of Patent: March 24, 2020
    Assignee: THERMO FINNIGAN LLC
    Inventors: Edward B. McCauley, Scott T. Quarmby
  • Patent number: 10600632
    Abstract: A method of operating an electrostatic trapping mass analyzer, comprises: (a) operating the electrostatic trapping mass analyzer at a maximum resolution so as to acquire a transient signal; (b) partitioning the transient signal into signal segments; (c) while a quality metric is either less than a pre-determined minimum threshold or greater than a pre-determined maximum threshold value, performing the steps of: (i) defining a test transient as being equal to either a first one of the segments or a previously defined transient with an appended signal segment; (ii) calculating a mathematical transform of the test transient and thereby generating a spectrum of component frequencies; and (iii) determining the quality metric from the spectrum of component frequencies; and (d) setting an instrumental resolution to be employed for subsequent mass spectral data acquisitions in accordance with a length of the most-recently-defined test transient.
    Type: Grant
    Filed: August 23, 2018
    Date of Patent: March 24, 2020
    Assignee: Thermo Finnigan LLC
    Inventor: Thomas D. McClure
  • Patent number: 10593529
    Abstract: A method and system for predicting in advance of treatment whether a cancer patient is likely, or not likely, to obtain benefit from administration of a yeast-based immune response generating therapy, which may be yeast-based immunotherapy for mutated Ras-based cancer, alone or in combination with another anti-cancer therapy. The method uses mass spectrometry of a blood-derived patient sample and a computer configured as a classifier using a training set of class-labeled spectra from other cancer patients that either benefited or did not benefit from an immune response generating therapy alone or in combination with another anti-cancer therapy.
    Type: Grant
    Filed: May 2, 2017
    Date of Patent: March 17, 2020
    Assignees: Biodesix, Inc., GlobeImmune, Inc.
    Inventors: Joanna Röder, Heinrich Röder