Abstract: An apparatus for automated examination of biological material (6) includes a microscope (8) with a cross table (9) with the biological material (6) being arranged on the cross table (9) between an object slide (1) or an analysis plate and a cover (4); a light source (15, 16); an evaluation unit; a device for automatic focusing (11); an image recording unit (17) for recording an image of the biological material (6) enlarged via of an object lens (10) of the microscope (8) and for transferring the image to the evaluation unit; and a biochip (2). Labeling (7) is provided on a surface of the biochip (2). Focusing is executable by targeted movement of the cross table (9) based on detection of the labeling (7), and a target interval is determinable by determination of a location and/or a position of the labeling, within which focusing of the biological material takes place.

Abstract: Provided is a microscope which includes an image pickup element, a light source, an optical system, a control unit and a sensor. The control unit controls for the acquisition of, in parallel with the first image pickup event by the image pickup element, necessary information when the second image pickup event by the image pickup element is performed, by using the sensor. A pair of sensors for a microscope includes a pair of sensors. A first sensor of the pair of sensors provides a signal that represents an environmental variable of a first area at a first period in time. A second sensor of the pair of sensors provides a signal that represents a quality of the first sensor's ability to represent the environmental variable of a second area at the first period in time, wherein the second sensor is adjacent to the first sensor.

Abstract: Some systems described herein include a frequency dependent phase plate for generating multiple phase-contrast images of a sample, each from a different frequency range of light, each phase-contrast image for frequency range of light formed from light diffracted by the sample interfered with undiffracted light that has a frequency-dependent baseline relative phase shift from the phase plate. In some embodiments, the multiple phase-contrast images may be used to generate a quantitative phase image of a sample. The phase-contrast images or the produced quantitative phase image may have sufficient contrast for label-free auto-segmentation of cell bodies and nuclei.

Abstract: A stability and, thus, also a quality of mosaic generation is improved by exploiting the advantages of global optimization while giving up performing the optimization fully on the basis of mutual alignment of the image information alone, i.e. on the basis of the result of a search for similarities, but when, additionally, information about error statistics of the capturing device, which took over portion-by-portion capturing of the object plane, is instead also taken into account in the positioning of the subimages in that a secondary constraint (secondary constraint) is set up for the capturing position variables in the optimization problem.

Abstract: Apparatus for and method of fully automatic rapid scanning and digitizing of an entire microscope sample, or a substantially large portion of a microscope sample, using a linear array detector synchronized with a positioning stage that is part of a computer controlled microscope slide scanner. The invention provides a method for composing the image strips obtained from successive scans of the sample into a single contiguous digital image. The invention also provides a method for statically displaying sub-regions of this large digital image at different magnifications, together with a reduced magnification macro-image of the entire sample. The invention further provides a method for dynamically displaying, with or without operator interaction, portions of the contiguous digital image. In one preferred embodiment of the invention, all elements of the scanner are part of a single-enclosure that has a primary connection to the Internet or to a local intranet.

Abstract: In accordance with the present invention there is provided a method for controlling a microscope to scan a microscope slide. By analyzing an overview image it is determined quantatively which swathe contains the most image detail and an optimum scanning order can subsequently be determined relative to the swathe determined as having the most detail. By scanning the swathe with the most detail first a good focus characteristic can be established for a dynamic focussing system and prediction errors in the dynamic focus system are likely to be low when scanning subsequent swathes.

Abstract: Optimal shading correction is achieved even when exposure times are different. An apparatus includes a section that stores a ? property of an image pickup device, a section that stores a shading pattern acquired by the image pickup device, and a first exposure time, a section that stores image data acquired by the image pickup device and a second exposure time, a section that calculates a pre-correction pattern, based on the stored shading pattern and the stored ? property, a section that calculates a revised pattern by performing ? correction for the calculated pre-correction pattern after multiplying the calculated pre-correction pattern by a ratio of the second exposure time to the first exposure time, and a section that performs shading correction for the stored image data by using the calculated revised pattern.

Abstract: The invention relates to a microscope having an illumination beam path with wide field illumination of a sample and a first detection beam path having a spatially resolved surface receiver, which is reached by a first part of the detection light coming from the sample via the first detection beam path, or an image divider assembly for a microscope. In order to lengthen the optical path length, at least a second part of the detection light coming from the sample is masked out of the detection beam path and, via deflection means belonging to the detection beam path, is led into a second detection beam path and, preferably via further deflection means, is deflected back in the direction of the detection in such a way that detection light is applied to at least two partial regions beside one another on the surface receiver.

Abstract: A cell counter includes: a sample slide configured to accommodate cells; a housing configured to be inserted into the inside of the sample slide from the outside of the sample slide; an object lens configured to be mounted within the housing, and to image-form a cell image for the cells projected from the sample slide; an image acquisition unit configured to be mounted within the housing together with the object lens, and to acquire the cell image image-formed by the object lens; and a first reflecting mirror provided between the sample slide and the object lens within the housing, and configured to change a projection direction of the cell image projected from the sample slide to the object lens.

Abstract: An image acquisition apparatus includes a test object stage that holds a test object, a measuring unit that acquires surface profile information of the test object, and a microscope unit that includes an objective optical system that forms an image of the test object and an image pickup element that captures the image of the test object formed by the objective optical system. The test object stage is movable between a measurement position of the measuring unit and a imaging position of the microscope unit. The measuring unit acquires first stage inclination information of the test object stage. At the imaging position, the test object stage adjusts an orientation thereof on the basis of a relationship between the surface profile information and the first stage inclination information.

Abstract: A microscope according to the present invention includes an imaging unit including a first illuminating unit, an imaging element, and a projection optical system, the first illuminating unit including a light source that illuminates a first object, the imaging element performing imaging of the first object, the projection optical system projecting an image of the first object onto the imaging element; a measuring unit configured to measure a second object for setting an imaging condition used when performing imaging of the second object at the imaging unit; and a controller configured to concurrently perform the imaging of the first object at the imaging unit and the measurement of the second object at the measuring unit.

Abstract: The purpose of the present invention is to provide an image forming apparatus and a computer program such that extraction of information about distortion in a charged particle beam scan area can be implemented. An embodiment for achieving the purpose proposes: an image forming apparatus that integrates image data obtained by a charged particle beam apparatus and that calculates, from a plurality of images with different scan directions of the charged particle beam apparatus, first information about the amount of change in a feature quantity in accordance with the time of irradiation of the charged particle beam, second information about the amount of change in the feature quantity before and after a change in beam scan direction, and/or third information about a position error of a pattern on the image before and after the change in beam scan direction; and a computer program for causing a computing apparatus to perform the above process.

Abstract: Provided is a method of analyzing a linearity of a shot image, including: irradiating a biological sample having a fluorescent label with an excitation light, the excitation light exciting the fluorescent label, and exposing an image sensor to light while moving a focus position of an optical system including an objective lens in an optical-axis direction and in a direction orthogonal to the optical-axis direction; and analyzing a gamma value for an imaging environment based on a brightness distribution of one bright-point image in a real-shot-image obtained by the image sensor.

Abstract: An image obtaining apparatus includes: a light source configured to irradiate a biological sample having a fluorescent label with excitation light, the excitation light exciting the fluorescent label; an optical system including an objective lens, the objective lens being configured to magnify an imaging target of the biological sample; an image sensor configured to form an image of the imaging target magnified by the objective lens; a movement controller configured to move a focus position of the optical system in an imaging range including at least a range corresponding to the thickness of the imaging target; and a data processing unit configured to exposure the image sensor to light while moving the focus position in the imaging range and obtain a fluorescent image of the biological sample, to thereby calculate distribution information of the fluorescent label in a thickness direction of the imaging target based on the fluorescent image.

Abstract: This invention provides an imaging device includes: a mosaic image generation unit which assembles a plurality of still images, and generates a mosaic image; a feature quantity extraction unit which extracts feature quantity from the frame image and the mosaic image; a relative position determination unit which determines a relative position between the frame image and the mosaic image, and a live image display unit which updates a display position of the frame image with respect to the mosaic image, and displays the moving picture image on the mosaic image. The live image display unit changes the display position in response to the relative position when succeeded in positioning of the frame image; and when failed in positioning, the live image display unit does not change the display position and fixes the display position near a position of a final frame image succeeded in positioning.

Abstract: A device and a method for acquiring a microscopic image of a sample structure are described. An optic for imaging the sample structure and a reference structure is provided, as well as a drift sensing unit for sensing a drift of the sample structure relative to the optic on the basis of the imaged reference structure. The optic comprises a first sharpness plane for imaging the sample structure and at the same time a second sharpness plane, modifiable in location relative to the first sharpness plane, for imaging the reference structure.

Abstract: An image obtaining apparatus includes: a light source configured to irradiate a biological sample having a fluorescent label with an excitation light, the excitation light exciting the fluorescent label; a focus moving unit configured to move a focus position of an optical system in the thickness direction of the biological sample; and a data processing unit configured to expose an image sensor to light while moving the focus position of the optical system in each of a plurality of preset scanning ranges to thereby obtain fluorescent images of the biological sample, each of the plurality of scanning ranges having a predetermined scanning length, the predetermined scanning length being smaller than the length of a bright-point detection range of the biological sample in the thickness direction, the center positions of the scanning ranges being different from each other in the thickness direction.

Abstract: An extended depth of field microscope system for phase object detection includes an imaging optical module and a phase/intensity converting module. The imaging optical module has an object lens group, in which an axial symmetric phase coding is added, to produce an axial symmetric spherical aberration. A point spread function (PSF) and an image with extended depth of field can be obtained with a predetermined level of similarity. The phase/intensity converting module converts the phase change of the light passing the phase object, into an image light with change of light intensity.

Abstract: The invention relates to a microscopy method for identifying target objects (32) having a predetermined optical property in material (6) to be analyzed.

Abstract: An illumination device and an observation system capable of outputting light having a continuous spectrum and high color rendering properties are provided. Employed is an illumination device including a first light source that emits first-wavelength-band light having a first wavelength band of violet color; a second light source that emits second-wavelength-band light having a second wavelength band that is broader than the first wavelength band and having a continuous spectrum; a light combining section that is composed of a dicroic mirror and that combines the first-wavelength-band light and the second-wavelength-band light; and a combination-ratio adjusting section that adjusts the combination ratio of the first-wavelength-band light and the second-wavelength-band light to be combined by the light combining section.

Abstract: An optical measurement system for measuring a critical dimension having a nanostructured surface including a nanostructure formed on a plane. The optical measurement system includes an image recording module including a microscope optical system which records a defocused image having an nonuniform degree of defocusing with respect to the nanostructured surface, an optical scheme parameter control module which sets and outputs to the microscope optical system optical scheme parameters for the microscope optical system, an image calculation module which receives receiving the optical scheme parameters set by the optical scheme parameter control module and calculates an image of the nanostructured surface, and a comparison module which compares the defocused image recorded by the image recording module and the image calculated by the image calculation module.

Abstract: A universal adapter system having a video camera mount and a digital camera mount for attaching a video camera and a digital camera to a single port of an optical observation device, such as a standard microscope, that allows for simultaneous video and digital camera imaging. An example digital camera mount includes a C-clamp attachment for attaching a standard, off-the-shelf digital camera and a sleeve attached to the adapter housing that allows use of an extendable zoom lens of the digital camera. The adapter may provide increased capabilities by use of a rotatable portion to allow positioning of the digital camera relative to the optical observation device, a parfocal zoom lens for simultaneous zoom adjustment of video and digital cameras, an auxiliary zoom lens, a fine focus adjustment for adjusting the video image independent of the digital image, a smartphone adapter, a Toric reticule and a light filter.

Abstract: A method for analysis of an object dyed with fluorescent coloring agents. Separately fluorescing visible molecules or nanoparticles are periodically formed in different object parts, the laser produces the oscillation thereof which is sufficient for recording the non-overlapping images of the molecules or nanoparticles and for decoloring already recorded fluorescent molecules, wherein tens of thousands of pictures of recorded individual molecule or nanoparticle images, in the form of stains having a diameter on the order of a fluorescent light wavelength multiplied by a microscope amplification, are processed by a computer for searching the coordinates of the stain centers and building the object image according to millions of calculated stain center co-ordinates corresponding to the co-ordinates of the individual fluorescent molecules or nanoparticles. Two-dimensional and three-dimensional images are provided for proteins, nucleic acids and lipids with different coloring agents.

Abstract: Multifunction autofocus for automated microscopy includes automatic coarse focusing of an automated microscope by reflective positioning, followed by automatic image-based autofocusing of the automated microscope performed in reference to a coarse focus position. In some aspects, the image-based autofocusing utilizes astigmatism in microscope optics for multi-planar image acquisition along a Z axis direction of a microscope. In some other aspects, the image-based autofocusing utilizes astigmatism in microscope optics in combination with chromatic aberration for multi-planar image acquisition along the Z axis direction.

Abstract: A microscope apparatus includes a ? stack image data acquisition unit that detects light emitted composed of a sample for each wavelength to acquire ? stack image data from multiple image data items for multiple different wavelengths; a spectrum generation unit that generates a spectrum for each pixel based on the ? stack image data; a clustering unit that performs clustering of the spectrum for each pixel into multiple clusters; a color setting unit that sets different colors to the clusters; and an image generation unit that generates an image of the sample by displaying pixels included in the clusters with a color set by the color setting unit. The distribution and gray scale of fluorescent materials within the sample can be correctly recognized and the state of a desired tissue can be favorably observed.

Abstract: The present invention relates to the field of digital pathology and in particular to whole slide scanners. A tilted autofocus image sensor images an oblique cross-section of the slide. For focusing multiple sequential overlapping images, which have been taken by the tilted sensor, are compared. The axial position of the tissue layer can be determined from the polar error signal resulting from this differential measurement.

Abstract: A microscopy method and apparatus includes placing a specimen to be observed adjacent to a reflective holographic optical element (RDOE). A beam of light that is at least partially coherent is focused on a region of the specimen. The beam forward propagates through the specimen and is at least partially reflected backward through the specimen. The backward reflected light interferes with the forward propagating light to provide a three dimensional interference pattern that is at least partially within the specimen. A specimen region illuminated by the interference pattern is imaged at an image detector. Computational reconstruction is used to generate a microscopic image in all three spatial dimensions (X,Y,Z), simultaneously with resolution greater than conventional microscopy.

Abstract: Provided is a magnification observation device in which a focus of the imaging unit can be focused on the observation surface of the object in observation object region in a short period of time. An unit region of an observation object is imaged at a plurality of Z-positions by moving a object lens in the light-axis direction, the plurality of pieces of image data corresponding to the unit region are captured. An image of the observation object is displayed in the display unit as a navigation image. Shape data which indicates the position of the surface of the observation object in the unit region is generated. An observation object region on observation object is designated based on the navigation image. Based on the generated shape data, a focus of the object lens is on the surface of the observation object on the observation object region designated.

Abstract: A line confocal microscope system, comprising a scanning unit in the form of a mechanically driven scanning unit with a controllable a scanning trajectory and a two dimensional sensor unit operated in a rolling line shutter mode in synchronization with the scanning unit, wherein the scanning trajectory is set to have an acceleration part outside the field of view of the sensor unit.

Abstract: An imaging system receives a height measurement and a resistance measurement, the measurements being made at a two-dimensional position on a surface. A display system maps the resistance to a predetermined material color value, and displays the height measurement and the material color value at the two-dimensional position on the surface.

Abstract: Provided is a magnification observation device capable of easily moving an observation object in a desired direction even when a stage is rotated, and capable of preventing a desired region of the observation object from going outside an imaging region due to rotation of the stage. The observation object is placed on the placement surface of the stage. The observation object is imaged by an imaging unit, to acquire image data of the imaging region. Based on the image data acquired by the imaging unit, an image of the observation object is displayed by the display part as an observed image. The stage is moved relatively with respect to the imaging unit along an xt-axis and a yt-axis. In this case, moving amounts along the xt-axis and the yt-axis are controlled based on a rotational angle detected by a rotational angle detecting part.

Abstract: A method and system for automatically evaluating quality of a slide-mounted tissue sample includes receiving a digital image of a magnified portion of the slide-mounted tissue sample. At least one quantitative quality indicator is automatically determined for at least one of the samples, and the digital image of the magnified portion of the sample. Each of the quantitative quality indicators is automatically compared to a respective minimum acceptable quality threshold. The quantitative quality indicators and associated quality thresholds are selected for suitability with an automated quantitative immunoassay. Failure of one or more of the quantitative quality indicators to meet its respective minimum acceptable quality threshold suggests that the sample is unsuitable for subsequent automated pathological evaluation.

Abstract: Super-resolution observation apparatus includes excitation light irradiation unit for irradiating excitation light intended to excite a sample on the sample, excitation light modulation unit for modulating a spatial intensity distribution of the excitation light on the sample, enlarged image forming unit for forming an enlarged image of the sample at an image position from observation light generated by irradiating the excitation light on the sample, image capturing unit for converting a spatial intensity distribution of the enlarged image into digital image data, and super-resolution processing unit for generating a super-resolution image where a super-resolution frequency component higher than a cutoff frequency of the enlarged image forming unit is made visible from one or a plurality of pieces of the digital image data.

Abstract: A microscope system includes a microscope, a digital camera and a computer system. The microscope has an automatically adjustable subassembly having an adjustable element. The digital camera acquires image data of an image of a specimen. The computer system has a display and a storage unit configured to store the image data and to store, associated with the image data, data defining a setting of the automatically adjustable subassembly corresponding to the image data.

Abstract: Imaging systems, such as optical microscopes, can benefit from automatic focus enhancements including sample detection. For example, systems that use a charge coupled device (CCD) video camera to capture a field of view for making focus determinations can benefit from automatic focus and sample detection. A method according to certain embodiments can include obtaining, by a machine, a high level image of a sample. The method can also include determining, by the machine, whether a plurality of auto-focus areas of a plurality of fields of view are aligned with a portion of the sample. The method can further include obtaining, by the machine, a low level image of the sample when the plurality of auto-focus areas are aligned.

Abstract: A microscope includes a zoom optical system zooming over a sample, a zoom driving unit moving the optical system along an optical axis, an imaging unit imaging an observation image of the sample through the optical system, thereby generating image data on the sample, and a display unit displaying an image corresponding to the generated image data. A touch panel on a display screen of the display unit accepts an input corresponding to a contact position of an object. A driving control unit outputs a driving signal for changing a zoom magnification of the optical system by setting a middle point between contact positions on the touch panel corresponding to two position signals responsive to an input of the different contact positions as a zoom center position fixed without depending on the zoom magnification of the optical system when the two position signals are output from the touch panel.

Abstract: A microscope system includes a microscope frame on which a stage is placed; and a camera head, in which an objective lens is attachable, for capturing an image of a specimen, the camera head being attached to the microscope frame by being fitted, and being slidable with respect to the microscope frame in a direction that is parallel with a surface of the stage placed on the microscope frame and orthogonal to an optical axis of the attached objective lens.

Abstract: A magnifying observation apparatus includes a switching unit for switching between a plurality of microscopies, an image generating unit for generating an image of a sample by capturing an image of the sample with the respective plurality of microscopies switched by the switching unit, and a display unit for displaying the image generated by the image generating unit. The display unit updates a display of the display unit each time a new image is generated by the image generating unit.

Abstract: A method and device for autofocusing in a microscope (11), wherein two preferably spot-shaped, markers (12) are generated on an object, the spacing (d) of the markers representing an indication of the defocusing of a working plane (9) in the object from the focal plane (10) of the microscope (11). A focus drive (6) displaces the working plane (9) into the focal plane (10) as a function of the marker spacing (d). In order to allow rapid and exact focusing to be performed, a detector (4) acquires an image of the markers (12) generated on the object, an evaluation unit (7a) determines the spacing of the markers (12), and a control unit (7b) adjusts, as a function of the determined marker spacing (d), the speed of the focus drive (6) at which the working plane (9) is displaced into the focal plane (10).

Abstract: Systems and methods analyzing body fluids contain cells including blood, bone marrow, urine, vaginal tissue, epithelial tissue, tumors, semen, and spittle are disclosed. The systems and methods utilize an improved technique for applying a monolayer of cells to a slide and generating a substantially uniform distribution of cells on the slide. Additionally aspects of the invention also relate to systems and method for utilizing multi-color microscopy for improving the quality of images captured by a light receiving device.

Abstract: The present invention relates to a system and a method of digitizing a moving slide (4). According to the invention, the slide (4) is displaced via stepper motors (6X, 6Y, 6Z) and the exposition of said slide (4) for imaging purposes is performed in synchronous with pre-set numbers of steppings (nx, ny, nz) accomplished by said stepper motors (6X, 6Y, 6Z) by activating a light source (2) that is capable of emitting a luminous flux high enough. Said light source (2) is preferably controlled directly by the stepper motors (6X, 6Y, 6Z).

Abstract: An optical modulator device (100), in particular for a spatio-temporally light modulated imaging system (200), comprises a light modulating micro-mirror device (110) comprising an array of mirror elements (111) arranged in a modulator plane (112), wherein each of the mirror elements (111) can be switched individually between first (111a) and second (111b) states with first and second tilting angles, resp., relative to a modulator optical axis (113) perpendicular to the modulator plane (112), and a first optical relaying device (120) being arranged for relaying light between the mirror elements (111) in the first (111a) state and a first optical axis (121) deviating from the modulator optical axis (113), wherein the first optical relaying device (120) includes a first group of imaging elements (122, 123, 124) being formed such that a planar light field (114) perpendicular to the modulator optical axis (113) is relayed to a first planar light field (125) perpendicular to the first optical axis (121).

Abstract: An information processing apparatus includes a storage unit, a determination unit, and a generation unit. The storage unit stores a plurality of partial images obtained by taking images with respect to a subject so that a plurality of image taking areas are overlapped with each other and relative positional displacement information of two adjacent partial images out of the plurality of partial images, the positional displacement information being calculated for each of the two adjacent partial images. The determination unit determines at least one display partial image for generating a display area image from the plurality of partial images, the display area image being an image of an area displayed as an image of the subject. The generation unit connects, when a plurality of display partial images are determined, the plurality of display partial images on the basis of the positional displacement information, to generate the display area image.

Abstract: Time lapse observation method includes: before a process to obtain a first time lapse image, capturing an image of a reference area on a sample being a partial area of a target area or an area in a vicinity of the target area being a smaller area than the target area to obtain a reference image; storing a position of a capturing area in capturing the reference image as a reference position; before a process to obtain the time lapse image performed, setting a position of the capturing area sequentially at different positions in the optical axis direction of an objective including the reference position and capturing an image at each of the positions to obtain comparison target images; and matching the capturing area with the target area, based on a comparison result of the reference image and the comparison target images.

Abstract: The present invention relates to the field of digital pathology and in particular to whole slide scanners. Autofocus imaging can be performed by sampling a first number of pixels of a primary image sensor and sampling a second number of pixels of an autofocus image sensor, wherein the second number is between one quarter and three quarters of the first number. Thus, continuous autofocus for rapid light scanning may be provided based on an additional image sensor that is tilted with respect to the optical axis.

Abstract: There is provided an imaging apparatus that includes a reception section, and an output section. The reception section is configured to receive a trigger from an external apparatus at a timing for reset and readout of an imaging element. The output section is configured to provide back, to the apparatus, a status indicating in which state the imaging element is when the reception section receives the trigger.

Abstract: Instrument for obtaining wide field images of a specimen comprising (1) a divergence disperser, generating within a light beam different divergences in each of the chromatic components thereof to form images of the specimen at different depths thereof, and (2) a wavelength analyser, selecting the wavelength or interval of wavelengths of the light beam to form the image of the specimen at a specific depth. The light beam may be mono- or polychromatic and the instrument may have a configuration of illumination by transmission or by reflection. The instrument may moreover be modified through diverse configurations of the divergence disperser and/or of the wavelength analyser, including the motorisation thereof or the inclusion of a movable mask, together with being coupled to a CCD or CMOS camera to integrate the diverse images acquired from all sections of the specimen.

Abstract: A method of analyzing a semen sample having living sperm is provided comprising receiving from a remote location a set of digital video clips of a prepared semen sample having living sperm and analyzing a subset of the digital video clips taken from the prepared semen sample using a Computer Assisted Semen Analyzer adapted to analyze the subset of digital video clips received from a remote location. A method of measuring sperm morphology is also provided comprising receiving from a remote location a digital video clip of a prepared semen sample having stained non-living sperm, and, analyzing portions of the digital video clip taken from the prepared semen sample.

Abstract: A microscope system includes a light amount ratio changing unit for changing a ratio of the amount of light directed to a first optical path for directing an optical image of the sample to an eyepiece lens and a second optical path for directing an optical image of the sample to an image capturing unit, an image capturing controlling unit for controlling an exposure time of the image capturing unit, and a controlling unit for obtaining a first exposure time from the image capturing controlling unit, for calculating a second exposure time on the basis of the first exposure time and a second ratio of the amount of light, and for controlling the image capturing controlling unit to set the second exposure time as the exposure time if the light amount ratio changing unit changes to the second ratio of the amount of light.

Abstract: Provided are a magnification observation device, a magnification observation method, and a magnification observation program in which connected image data can be efficiently obtained in a short period of time when re-imaging an object to obtain the connected image data corresponding to a plurality of unit regions. A plurality of unit regions on a surface of an observation object are imaged, and a plurality of pieces of image data respectively corresponding to the plurality of unit regions are generated. An image of the object including the plurality of unit regions is displayed as a region presentation image. When any of the plurality of unit regions is selected by a selection instruction from a user, the selected unit region is re-imaged, to generate image data corresponding to the selected unit region as re-imaged data.