Abstract: A system having a macroscopic imager, a microscopic imager, and a stage for moving a substrate supporting ex-vivo tissue with respect to each of the imagers to enable the macroscopic imager to capture macroscopic images, and the microscopic imager to capture optically formed sectional microscopic images on or within the tissue, when presented to the tissue, via the optically transparent material of the substrate. A computer system controls movement of the stage, and receives the macroscopic and microscopic images. A display is provided for displaying the macroscopic and microscopic images when received by the computer system. The tissue is verified as being in an orientation at least substantially flush against the upper surface of the substrate by being in focus in displayed macroscopic images prior to imaging by the microscopic imager, and if needed, any portion of the tissue unfocused is manually positioned until desired tissue orientation is achieved.

Abstract: A micro-displacement amplifying apparatus comprises two sets of asymmetrical amplifying structures; each set of asymmetrical amplifying structure comprises a plurality of asymmetrical amplifying units connected in series by flexible hinges; the asymmetrical amplifying unit is used for amplifying a micro-displacement; the two sets of asymmetrical amplifying structures are in opposite positions and overlap with each other; the input end and output end are coupled to the asymmetrical amplifying unit by a flexible hinge, respectively; the input end is used for inputting the micro-displacement to the asymmetrical amplifying unit, and the output end is used for outputting the amplified displacement; the two contacting input ends are fixed and coupled to each other, and the two contacting output ends are fixed and coupled to each other. The present disclosure further discloses an amplification method of the micro-displacement amplifying apparatus.

Abstract: A method and system for measuring the alignment between a substrate and a platform upon which it is disposed by using image processing algorithms are described herein. These algorithms automate the detection of edges of a microscope slide and the platform in a digital image. A reference line pattern in an image of the platform can be used to detect platform edges based on a computed location of the reference line pattern in the image.

Abstract: An oscillation actuator of the present disclosure includes an oscillation body, a contact body configured to be in contact with the oscillation body, a holding member configured to hold the oscillation body, the holding member having a through hole, a pressing member configured to press the oscillation body through the through hole, and an oscillation damping member configured to be in contact with the holding member and the pressing member between the holding member and the pressing member.

Abstract: An apparatus can include a vessel, a reference surface, a preload, a scan actuator, and a transmitter. The reference surface can form a structural loop with a detector. The preload can be configured to urge the vessel to contact an area on the reference surface. The scan actuator can be configured to slide the vessel along the reference surface in a scan dimension. The transmitter can be configured to direct signal from the vessel to a detector and/or direct energy from an energy source to the vessel, when the vessel is urged by the preload to contact the reference surface.

Abstract: An apparatus can include a vessel, a reference surface, a preload, a scan actuator, and a transmitter. The reference surface can form a structural loop with a detector. The preload can be configured to urge the vessel to contact an area on the reference surface. The scan actuator can be configured to slide the vessel along the reference surface in a scan dimension. The transmitter can be configured to direct signal from the vessel to a detector and/or direct energy from an energy source to the vessel, when the vessel is urged by the preload to contact the reference surface.

Abstract: This disclosure is directed to system for transferring a substrate, such as a microscope slide, and holding the substrate within at least one device. The system includes a holder for holding the substrate and a gripper for transferring the substrate, such as between a cassette or stack and the holder. A method is also discussed herein.

Abstract: Rib retraction devices and methods of its use and manufacture are disclosed. A retraction device may include a first frame portion and a second frame portion. The second frame portion may be attached to the first frame portion. The second frame portion may mechanically move between a first position and a second position with respect to the first frame portion. The retraction device may also include a first blade being pivotally attached to the first frame portion and comprising a flexible material. The retraction device may also include a second blade being pivotally attached to the second frame portion and comprising a flexible material.

Abstract: An observation device according to the present invention comprises a stage on which a fluid vessel containing therein an object to be observed such as a cell or microorganism is to be placed, an observing part observing the object to be observed, a driving part performing a stage driving for moving a position of the stage with respect to the observing part, and a control part controlling the stage driving performed by the driving part. The stage driving includes a moving mode for moving the stage toward a predetermined position and a stop process mode for performing a stop process of stopping the stage at the predetermined position, and the control part performs the stop process several times in the stop process mode, and controls the stage driving so that a velocity or an acceleration of the stage driving is common among the stop processes in the stop process mode.

Abstract: An apparatus for transmitted light illumination for light microscopes A diaphragm edge may be variably positioned in the direction of the optical axis, wherein a position of the diaphragm edge in the direction of the optical axis can be varied irrespectively of a position of the diaphragm edge transversely to the optical axis. A separate sample support table may be mounted on a housing. The housing has a passage opening, through which the diaphragm edge can be moved in the direction of the optical axis. A holding device is formed in the region of the passage opening of the housing or on a separate sample support table and a control device is present which is adapted to position the diaphragm edge in dependence at least upon a determined presence of a sample support table.

Abstract: A vibration type driving apparatus including: a vibrator including an electro-mechanical energy conversion device; a driven body between the vibrator and a movable body and driven in a first direction by the vibrator; and a moving mechanism that allows the movable body to move in a second direction relative to the driven body, in which the second direction is a direction intersecting the first direction in a plane parallel to a plane where the vibrator and the driven body are in contact with each other.

Abstract: A microscope includes an illumination system, a stage, and an objective lens that forms an image of a specimen. The stage includes a fixed member whose position and inclination are fixed, a specimen holding member at least one of whose position and inclination is changeable, and a supporting member that supports the specimen holding member. A connection between the supporting member and the specimen holding member and a connection between the supporting member and the fixed member reside near sides of the specimen holding member. The microscope further includes an actuator that changes at least one of the position and the inclination of the specimen holding member with respect to the fixed member, and a buffer mechanism that suppresses transmission of any changes in the inclination of the specimen holding member to the actuator. The actuator and the specimen holding member are connected with the buffer mechanism interposed therebetween.

Abstract: A Raman microspectrometer system extends the optical reach and analysis range of an existing Raman microspectrometer to allow analysis and/or repair of an oversized sample. The Raman microspectrometer system includes an extender for extending the optical reach of the existing microspectrometer and a supplemental stage which extends the analysis range of the existing microspectrometer by providing travel capabilities for non-destructive analysis of an entire oversized sample. Such an arrangement decreases manufacturing costs associated with testing oversized samples such as mammography panels, enabling analysis and/or repair to be performed without destruction.

Abstract: A laser microscope that efficiently performs 3D imaging irrespective of the shape of a specimen, includes an area segmenting section that segments an observation range of a specimen in the direction perpendicular to the optical axis of an objective lens into many areas; a surface-position storing section that stores motorized stage positions in association with the specimen surface positions, for the many areas; a surface-shape estimating section that estimates the specimen surface shape from the motorized stage positions and the specimen surface positions for the many areas; a z-scanning condition determining section that determines the specimen surface positions at desired positions of the motorized stage from the specimen surface shape; and a light detecting section that detects light from the specimen over certain ranges specified with reference to the specimen surface positions, in the optical axis direction of the objective lens.

Abstract: The focus control culture observation apparatus enable simple and reliable detection of a focusing position of an objective lens on a subject and with certainty. When a focusing position is detected, an area, which includes an edge portion of the culture medium drop is selected as an AF area which is to be a target of detecting the focusing position of the objective lens on the culture medium drop. The apparatus detects the focusing position on the culture medium drop based on an observation image of the AF area. By detecting the focusing position as a targeting area that is different from an area which is near the edge of the culture container and where illumination unevenness is generated, the detection accuracy can be improved.

Abstract: Disclosed herein is a stage system, including: a stage on which to mount a slide glass; a projection block projected more than the thickness of the slide glass to the side of that surface of the stage on which to dispose the slide glass; and a pressing block which is provided on that surface of the stage on which to dispose the slide glass, is thicker than the slide glass, and presses toward the projection block the slide glass disposed between itself and the projection block.

Abstract: An instrument and method for scanning at least a portion of a large specimen preferably causes the specimen to move relative to a two-dimensional detector array at a constant speed. The detector array takes one image of the specimen for each line that the detector moves. A controller controls a shutter of the detector array to open to take images and to pass the images to a processor, which is preferably a computer. The instrument takes one partial image of each part of the specimen that is being scanned and then combines those images with other images to produce a contiguous image.

Abstract: A device and method for adjusting mounting of a microscope stage (14), to which an objective (38) is mounted, to a microscope stand (12), including at least one first connecting element (56) arranged on the microscope stand (12), at least one second connecting element (62) arranged on the microscope stage (14) for mounting the microscope stage (14) to the microscope stand (12), at least one first mating part (76) arranged on the microscope stand (12), at least one positioning base (66) supporting a second mating part (74), wherein the positioning base (66) adjusts the platform (16) on the microscope stand (12) and is mounted movably in a plane of adjustment that is parallel to the platform (16) with the mating parts (74, 76) being engaged, and at least one locking device (80) for locking the positioning base (66) to the platform (16).

Abstract: A microscope controller improves the operability in moving a stage when a stage moving operation is performed on a touch panel. The microscope controller for control of the operation of each electric unit configuring the microscope system solves problems by including: a touch panel unit having a display function and receiving an input by an external physical contact; a control unit for setting a display region as a function area by assigning an image for operation of the electric unit to a specified display region of the touch panel unit, and, when an input to the function area is detected, generating a control instruction signal for control of the electric unit depending on the contact operation to the electric unit corresponding to the function area, and a communication control unit for transmitting the control instruction signal to an external device for controlling the operation of the electric unit.

Abstract: The image pickup apparatus includes an image sensor unit including an optical system forming an optical image of an object placed on a stage part and plural image sensors each of which captures part of the optical image, a drive mechanism relatively moving the stage part and the image sensor unit, and a processing part causing the image sensor unit to perform plural image capturing operations with causing the drive mechanism to relatively move the stage part and the image sensor unit after each image capturing operation, and combining the plural captured images to produce a whole image covering the whole image capturing area. The plural image sensors are arranged such that an effective diameter of the optical system necessary to introduce light from the stage part to all the plural image sensors is smaller than a diameter of a circle circumscribed to the whole image capturing area.

Abstract: A microscope stage (14) comprising a platform (16), a specimen holder (18) resting on the platform (16) and a positioning device (20) for moving the specimen holder (18) in a plane of displacement parallel to the platform (16) is described. The microscope stage (14) includes a positioning device (20) having two displacing devices (34, 36) which are mechanically decoupled from each other and of which a first displacing device (34) is designed to move the specimen holder (18) along a first axis in the plane of displacement, and a second displacing device (36) is designed to move the specimen holder (18) along a second axis in the plane of displacement, which second axis runs transversely to the first axis.

Abstract: An X-Y stage for microscopes comprises a base stage element, an intermediate stage element, and an upper stage element. The upper stage element is mounted to the intermediate stage element and is displaceable along a first axis. The intermediate stage element is mounted to the base stage plate and is displaceable along a second axis. A mechanical stage drive comprises an upper stage element drive with an output link coupled to the upper stage element and providing a first drive direction. An intermediate stage drive with an output link coupled to the intermediate stage and providing a second drive direction. The X-Y stage further comprises at least one of a first mechanical switch for reversing the first displacement direction and a mechanical second mechanical switch for reversing the second displacement direction.

Abstract: A fiber optic illuminator is provided comprising an optics bed, a light source, a collimating lens, and a condensing lens. The light source is mounted in a fixed position with respect to the optics bed. The collimating lens is mounted in a fixed position with respect to the optics bed and collimates at least a portion of the light from the light source. A condensing lens receives the substantially collimated light output and focuses the collimated light output to optically couple to an optical fiber. The condensing lens may be mounted on an adjustable mount. The tolerances of the fixed optical elements i.e. light source and collimating lens allow the fixed optical elements to be positioned with a minimal amount of variation where the variation is determined by manufacturing tolerances associated with these individual elements and their mounts and couple them to the optics bed.

Abstract: A manually operated driver for precision support stages having coarse and optional fine position control. A yoke connected to a movable stage slides along a shaft parallel to the direction of motion of the stage. The yoke has opposing pivot members that can, in one position, contact the shaft to self-lock the position of the yoke on the shaft and, in another position, remain clear of the shaft thereby allowing the yoke to slide freely on the shaft for coarse position control. The shaft has threads at one end that turn into a support block. Turning of the shaft with a knob and with the yoke locked to the shaft, slowly changes the position of the yoke and the connected stage for fine position adjustment of the stage. A pair of such drivers in orthogonal orientation on parallel x-y movable stages will provide two dimensional motion of stages.

Abstract: An apparatus for adjusting a microscope stage. The microscope stage of the apparatus defines a plane on which an object to be examined can be placed. The apparatus further includes a first shaft and a second shaft rotationally coupled to the first shaft. The microscope stage is guided between the first and second shafts such that a rotational movement of at least one of the first and second shafts has a direct effect on the microscope stage.

Abstract: An apparatus for adjusting a tension of a pulling device in a pulling-device drive includes a body and a lever arm. The pulling-device drive includes first and second shafts and the pulling device loops around the first and second shafts. The body includes a receptacle adapted to interact with the first shaft and a support point adapted to rotatably dispose the body about an axis of rotation. The lever arm extends from the body so that a spacing between the first and second shafts is modifiable by action of a force on the lever arm.

Abstract: Apparatus for single-handed control of a microscope are disclosed. In one embodiment, a first controller, such as a first rotary controller, is configured for manipulation by a hand of a user to adjust a position of a microscope stage. Another controller, such as a second rotary controller, is configured and arranged relative to the first rotary controller for manipulation by an extended finger of the same hand to review, e.g., scroll through, previously identified portions of a biological specimen while the user's first hand may or may not remain in contact with the first rotary controller. In this manner, the user may continue to review the previously identified specimen portions without having to look away from the microscope.

Abstract: A microscope slide separation device and method of storing microscope slides are disclosed. In specific embodiments, the microscope slide separation device can be placed a folded position that forms a cavity between at least first and second members configured to receive a microscope slide to which both a sample and a slip cover have been coupled. In particular embodiments, the first member may have at least one side configured not to stick to cover slip mounting media.

Abstract: Control apparatus for a microscope having at least one rotary control member for adjustment of the microscope, the apparatus comprising: a motor; a coupling arrangement driven, in use, by the motor; and a mount arrangement to provide adjustable positioning of the motor relative to the control member, to cause the coupling arrangement to engage the rotary control member to cause the control member to be driven by rotation of the motor.

Abstract: The present invention facilitates improvements in laser ablation of solid samples to be analyzed by an external inductively coupled plasma (ICP) emission spectrometer, ICP/mass-spectrometer (ICP-MS), or flowing afterglow (FAG) mass spectrometer (FAG-MS) for elemental analysis (ICP and ICP-MS) or molecular analysis (FAG-MS). A novel invention mirror-with-hole beam combiner eliminates chromatic aberration in the invention sample view and allows rad-hardening the laser ablation invention for use in a radiation hot cell for analysis of high activity nuclear waste. Many other novel invention rad-hardening attributes facilitate a comprehensive rad-hardened laser ablation system (the world's first).

Abstract: A sample positioning stage for positioning a sample to be inspected relative to an optical inspection device. The stage includes a first generally planar body on which a sample to be inspected can be carried and a second body directly coupled to the first body via bearings extending between them which constrain movement of the first body relative to the second body to a first plane that is substantially parallel to the plane of the first body. There is also provided a drive system being selectively operable in a first mode and a second mode.

Abstract: A scanning system that provides for detection based on supercritical angle fluorescence (SAF) is described. The system provides for the optical coupling of a sample to the scanner in a sandwich structure that uses first and second refractive index matching materials to provide optical coupling through the sandwich arrangement.

Abstract: The focus control culture observation apparatus enable simple and reliable detection of a focusing position of an objective lens on a subject and with certainty. When a focusing position is detected, an area, which includes an edge portion of the culture medium drop is selected as an AF area which is to be a target of detecting the focusing position of the objective lens on the culture medium drop. The apparatus detects the focusing position on the culture medium drop based on an observation image of the AF area. By detecting the focusing position as a targeting area that is different from an area which is near the edge of the culture container and where illumination unevenness is generated, the detection accuracy can be improved.

Abstract: There is disclosed a method and apparatus for acquiring stereoscopic images. A first camera may be mounted to a first convergence plate, the first convergence plate coupled to a first plate via a first XY slide and a first pivot displaced from the first XY slide. A distance between the first plate and a second plate may be adjusted to set an interocular distance between the first camera and a second camera. The first convergence plate may be rotated about the first pivot to set, at least in part, an angle of convergence between the first camera and the second camera.

Abstract: A system for acquiring and displaying images of preparations is provided. The system includes a first and second illumination device, each including an illumination source defining a respective illumination beam path amid configured to emit light to illuminate the preparation. A camera and a display for displaying a portion of the preparation are further provided. The preparation can be placed on a stage that is movable in x and y coordinate directions. The frame is also provided with a combining device configured to combine the first imaging beam path and the second imaging beam path, and direct them to the camera.

Abstract: Provided with a time-lapse imaging unit which repeatedly captures a specimen at predetermined time intervals and generates a plurality of images, and a recording unit which records at least one of an image group including one or more of the images captured during a predetermined period among a period of a time-lapse capturing performed by the time-lapse imaging unit or an image group including one or more of the images picked at predetermined time intervals among the period of the time-lapse capturing performed by the time-lapse imaging unit. Thus, data generated in time-lapse photography are managed favorably in a microscope apparatus provided with a time-lapse imaging unit which repeatedly captures a specimen at predetermined time intervals and generates a plurality of images.

Abstract: A stage drive for moving a microscope stage designed as a mechanical stage including a first drive element linked to a first output element, and a second drive element, which is linked to a second output element. Through first and second transmission elements, the first output element is linked to a first stage element and the second output element is linked to a second stage element of the mechanical stage supported by the first stage element. The stage elements are supported so as to be displaceable relative to a fixed base element in orthogonal directions X, Y in a plane extending normal to the optical axis of the microscope beam path. The at least one stage drive includes at least one drive element and at least one output element and is arranged in the base element so as to be displaceable in X direction and/or Y direction within an adjustment range and lockable.

Abstract: A microscope control unit which is composed of a bracket-shaped support frame having a support area, a pillar standing vertically erect on the support area, and a support arm, which emanates from the upper end of the pillar and is aligned parallel to the support area.

Abstract: A comparison optical system (1) comprising several image-acquiring optical subsystems is disclosed. A bridge (3) mechanically and optically connects the optical subsystems to one another. Each of the image-acquiring optical subsystems possesses an XYZ stage (8a, 8b), movable in motorized fashion, on which a sample to be examined is placed. Also provided is a control unit which moves the XYZ stages (8a, 8b), movable in motorized fashion, synchronously in all three spatial directions. The synchronous motion of the XYZ stages (8a, 8b) can be switched on and off by the user.

Abstract: A microscope system comprises a microscope including a motorized stage on which is mounted a container containing a specimen and which can adjust the position of the container, a scanner scanning laser light radiated onto the specimen, an objective lens focusing the scanned laser light, an image-acquisition unit acquiring a specimen image by detecting fluorescence produced in the specimen, and a dark box containing these components; a storage unit storing the mounting position of the container on the motorized stage; an image-acquisition-position setting unit setting acquisition positions of partial images of the inside of the container, on the basis of the stored mounting position of the container; a control section controlling the microscope so as to acquire the partial images for each container on the basis of the set acquisition positions; and a map-image generating section arranging the partial images to generate a map image.

Abstract: A microscope control unit which is composed of a bracket-shaped support frame having a support area, a pillar standing vertically erect on the support area, and a support arm, which emanates from the upper end of the pillar and is aligned parallel to the support area.

Abstract: A device for analysing a specimen by fluorescence includes a confocal microscope, illumination means capable of emitting a light beam that converges, by means of an objective, on a focal spot, means for successively positioning the focal spot at various points on the specimen during analysis. The confocal microscope includes an objective mounted on a movable rapid-scan carriage driven in a reciprocating linear movement along a traverse direction by a rotating motor by means of a device of the connecting rod type. The specimen is placed on a movable support driven in a longitudinal movement and is able to move along the axis of the objective of the microscope in order to position the specimen relative to the focal spot.

Abstract: In a flexure arrangement for translating positioning of an object in a microscope system, two actuators both act along a first direction, while the object partly can be positioned along the first direction and partly, with a gear ratio, along the second direction, which is perpendicular to the first.

Abstract: A microscope stage capable of always heating the entire culture vessel even when the culture vessel is moved in two-dimensional directions, and allowing an object lens and a condenser even in a high-power microscope to approach an object of observation such as a cell until they come into focus. A heating unit (58) faces a well plate (37) on a drive base (49) even when the drive base (49) is driven to any position in two-dimensional directions. Therefore, all the cells A in the small compartments (45) of the well plate (37) are always heated. Since the microscope stage (25) is constituted such that a lower-side base (71) houses an upper-side base (73) and a fixed base (47) in recessed portions, its thickness is considerably small. Accordingly, an object lens (5) and a condenser (3) can approach close to the cells A. Therefore, it is possible for high-power object lens (5) and condenser (3) to focus on the cells A.

Abstract: A motorized table apparatus comprises: a support unit for supporting a movement table so as to allow it to move in a single axis direction relative to a stationary unit which is fixed to the movement table; a slide member which is equipped on the movement table and which has a length equivalent to, or more than, a moving range of the movement table; an ultrasonic oscillators which is equipped on the stationary unit, which moves the movement table, and which comprises a single piezoelectric body and two drive elements; and a pressure application unit for supporting so that the two drive elements of the ultrasonic oscillator and the slide member are pressed by a constant force, wherein a plurality of the ultrasonic oscillators are equipped.

Abstract: An observation device according to the present invention comprises a stage on which a fluid vessel containing therein an object to be observed such as a cell or microorganism is to be placed, an observing part observing the object to be observed, a driving part performing a stage driving for moving a position of the stage with respect to the observing part, and a control part controlling the stage driving performed by the driving part. The stage driving includes a moving mode for moving the stage toward a predetermined position and a stop process mode for performing a stop process of stopping the stage at the predetermined position, and the control part performs the stop process several times in the stop process mode, and controls the stage driving so that a velocity or an acceleration of the stage driving is common among the stop processes in the stop process mode.

Abstract: A microscopy system includes an image focusing module, a stage for supporting a sample, image collection unit for collecting sliced images of the sample acquired by the image focusing module, and an image fusion unit for fusing sliced images of the sample acquired from different observation angles. The stage supports the sample and is configured to be revolvable around a rotational axis which is substantially perpendicular to an extending direction from the sample to the image focusing module so that enabling the image focusing module to acquire sliced images of the sample from different observation angles. The image fusion unit is used for remapping the sliced images acquired from different observation angles into a reference coordinate system, converting anisotropic voxels resolution of the sliced images to isotropic resolution, and fusing the sliced images into a final image.

Abstract: The invention relates to a microscopic cell observation and inspection system that uses a total internal reflection cell illuminator that is capable of freely changing an observation position without recourse to any special slide glass, makes sure high SN-ratio observation and facilitates sample manipulation, thereby making high-sensitivity, fast detection of a lot of cell reactions on the same slide glass. While, in response to a command from personal computer (80), step motors (53, 54) are driven to sequentially scan observation positions of cell sample (S) on slide glass (21), one of shutter units (71) and (72) is closed and the other is opened at high speed, whereby either one of illumination optical paths for a TIRF microscope and a drop fluorescence microscope is selected to illuminate cell sample (S) on that observation position.

Abstract: A microscope plate indexing apparatus includes a base platform and an indexing platform. The indexing platform has a slideway to slidingly receive a crystallography or other microscope plate. The indexing platform includes two projecting pins, one of which is a guide pin received in a guide groove in the base platform and the other of which is an indexing pin received in one of a plurality of indexing holes in the base platform. The indexing holes are preferably located in an indexing groove. The guide groove keeps the indexing platform aligned with the base platform during movement of the indexing pin from one indexing hole to the other and maintains the indexing platform in substantially fixed position in relation to the base platform when the indexing pin is in a selected indexing hole to insure correct alignment of the two platforms.

Abstract: A positioning mechanism capable of decreasing width and size and a microscope using the positioning mechanism are provided. The positioning mechanism is provided with a supporting shaft possessing a spherical surface part at a prescribed position and guides disposed substantially parallel to the supporting shaft and is characterized, on the assumption that of the three-dimensional directions, the axial direction of the supporting shaft constitutes the z direction, by the fact that the supporting shaft is nipped by the guides at the position of the spherical surface part and enabled to oscillate on the spherical surface part as a fulcrum in the xy directions and as well slide in the z direction.