Porous Media Patents (Class 422/535)
  • Patent number: 10324036
    Abstract: The invention relates to a porous planar cell capture system for use in determining the presence and/or amount of cells, for example, viable cells, in a liquid sample, and to methods of using such a cell capture system. The cell capture system contains a fluid permeable, planar membrane adopted to retain cells thereon, a fluid permeable support member that supports the membrane, and an optional register associated with the membrane.
    Type: Grant
    Filed: May 2, 2013
    Date of Patent: June 18, 2019
    Assignees: Charles River Laboratories, Inc., ReaMetrix Inc.
    Inventors: Norman R. Wainwright, Bala S. Manian, Eric Stimpson, Brian J. Kolonia, Robert K. Kolonia
  • Patent number: 10031052
    Abstract: An apparatus (100) for processing a biological sample (101). The biological sample being arranged on a first planar surface (102) of a carrier (103). The apparatus comprises a second planar surface (104) arranged substantially parallel to said first planar surface and at a first distance from said first planar surface, said first planar surface and said second planar surface are arranged at an angle (A) greater than zero degree from the horizontal plane (HP); supply means (126, 131, 220) for supplying an amount (105) of a liquid that is to be applied to said biological sample. The first planar surface and second planar surface are configured to be arranged in a second distance from each other, said second distance being such that said supplied amount of liquid is distributed over said biological sample when said first planar surface and said second planar surface are brought to said second distance from each other.
    Type: Grant
    Filed: October 14, 2015
    Date of Patent: July 24, 2018
    Assignee: DAKO DENMARK A/S
    Inventors: Tim Svenstrup Poulsen, Steen Hauge Matthiesen
  • Patent number: 9879090
    Abstract: The present invention relates to isolated VHHs directed against human Glycophorin A. The present invention also relates to fusion proteins comprising the VHH according to the invention that is fused to at least one heterologous polypeptide and immunoconjugates comprising the VHH according to the invention that is conjugated to at least one chemical compound and their use in therapeutic or diagnostic methods.
    Type: Grant
    Filed: March 4, 2014
    Date of Patent: January 30, 2018
    Assignees: INSERM (Institut National De La SantéEt De La Recherche Médicale, Imoversité Paris Diderot—Paris 7, Universite Des Antilles Et De La Guyane, Institut National De La Transfusion Sanguine (INTS)
    Inventors: Olivier Bertrand, Ibrahim Habib, Dorota Smolarek
  • Patent number: 9494500
    Abstract: A system and method thereof for collecting and concentrating a biologic substance of interest is provided. The biologic of interest obtained from a biologic sample present at an initial low concentration (or low number counts) can be captured and released through a collection device of the system to an intermediate second concentration, and further recovered through a concentration device of the system to a third concentration, thereby facilitating subsequent detection, characterization, enumeration, immunostaining, inspection, imaging, culturing, molecular analysis, and/or other assays.
    Type: Grant
    Filed: October 28, 2013
    Date of Patent: November 15, 2016
    Assignee: Academia Sinica
    Inventors: Ying-Chih Chang, Chia-Hsien Hsu
  • Patent number: 9476886
    Abstract: A sensor for detecting and/or quantifying the amount of analyte in a sample, the sensor including: a sensing region; and a barrier layer including a reactive oxygen species (ROS)-quenching, analyte-permeable membrane having an ROS-quenching agent adsorbed thereto; wherein the sensor is adapted so that the sample enters the sensing region of the sensor through said barrier layer.
    Type: Grant
    Filed: January 26, 2015
    Date of Patent: October 25, 2016
    Assignee: Lightship Medical Limited
    Inventors: William Paterson, Nick Barwell, Bruce Culbert, Barry Colin Crane
  • Patent number: 9329110
    Abstract: The present disclosure relates to a distribution apparatus for a sample exposition arrangement comprising at least two sample containers and a fluid source for fluid to be supplied to the sample containers. The distribution apparatus includes a distribution device including a distribution device inlet for supplying fluid to the distribution device, one distribution device outlet each per sample container, and a distribution chamber between the distribution device inlet and the distribution device outlets. The distribution apparatus further includes at least one fluid outlet device per distribution device outlet. The at least one fluid outlet device includes one fluid-outlet device inlet in fluid communication with the corresponding distribution device outlet, a first fluid-outlet device outlet for discharging fluid into the corresponding sample container, and a second fluid-outlet device outlet for discharging excess fluid.
    Type: Grant
    Filed: November 20, 2012
    Date of Patent: May 3, 2016
    Assignee: PIETER VAN WEENEN & CO. GmbH, The House of Innovation
    Inventor: Tobias Krebs
  • Patent number: 9249661
    Abstract: An apparatus and a method including exposing a first fluid to a pre-filter, observing the first fluid, introducing a second fluid to the first fluid, exposing the first and second fluids to a filter, and observing the first and second fluids wherein the observing the first fluid and observing the first and second fluids comprise optical measurements and the first fluid comprises material from a subterranean formation. Some embodiments may compare the optical measurements of the first fluid and the first and second fluids and/or estimate the first fluid's likelihood of forming precipitants with other fluids and/or the first fluid's asphaltene content.
    Type: Grant
    Filed: January 20, 2012
    Date of Patent: February 2, 2016
    Assignee: SCHLUMBERGER TECHNOLOGY CORPORATION
    Inventors: Christopher Harrison, Farshid Mostowfi, Matthew T. Sullivan, Elizabeth Smythe, Abdel M. Kharrat
  • Patent number: 9170258
    Abstract: The present invention is an improved apparatus for sample analysis. The apparatus employs an assay component containing a membrane having one or a plurality of analyte-specific binding agents attached thereto, a means for absorbing liquid, and a piston means for drawing analytes through said membrane into said means for absorbing liquid. The apparatus is configured to be portable and provide a detector for detecting binding of an analyte to an analyte-specific binding agent, a plurality of data acquisition components, and a computer for integrating, analyzing and storing the detected analyte specific binding and acquired data.
    Type: Grant
    Filed: November 25, 2014
    Date of Patent: October 27, 2015
    Assignee: Montecito Bio Sciences, Ltd.
    Inventors: Edward W. Withrow, III, Jorn Gorlach
  • Publication number: 20150126724
    Abstract: A fluid sample extraction device is disclosed comprising a housing defining an internal fluid passage having a distal open end and a proximal open end and a porous medium within the fluid passage, between the distal open end and proximal open end. The porous medium, which may be glass, is configured to allow fluid flow in the fluid passage to flow around the porous medium, toward the proximal open end of the housing, when fluid is drawn from the distal open end toward the proximal open end, and to allow fluid in the fluid passage to flow toward the distal open end of the housing, through the porous medium when the fluid is forced toward the distal open end. The porous medium is also configured to capture nucleic acids in the porous medium from the fluid when fluid is forced through the porous medium. Devices and kits are also disclosed.
    Type: Application
    Filed: October 31, 2014
    Publication date: May 7, 2015
    Inventors: Marc Dominic De John, Jesse Wilson van Westrienen
  • Publication number: 20150118728
    Abstract: According to embodiments of the present invention, an apparatus for separating a biological entity from a sample volume is provided. The apparatus includes an input chamber including an inlet configured to receive the volume sample, and an outlet, at least one magnetic element adjacent a portion of the input chamber, the magnetic element configured to provide a magnetic field in a vicinity of the portion of the input chamber to trap at least some leukocytes from the sample volume, and a filter in fluid communication with the outlet, the filter configured to separate the biological entity. According to further embodiments of the present invention, a method for separating a biological entity from a sample volume is also provided.
    Type: Application
    Filed: April 19, 2013
    Publication date: April 30, 2015
    Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
    Inventors: Abdur Rub Abdur Rahman, Chandran Jegatha, Lakshmi Shankar, Chee Chung Wong
  • Patent number: 9005543
    Abstract: An apparatus for punching and extracting analytes from a dried biological fluid spot includes a tube and a sorbent bed. The tube includes a proximal tube opening, a distal tube opening, and a distal section. The distal section includes a tube wall having a tapered diameter that reduces down to the distal tube opening. The apparatus may also include a tube extension that includes an extension wall having a tapered diameter that reduces from a proximal extension opening to a distal extension opening. The tube extension is movable between an attached position and a detached position. At the attached position, the tube extension is secured to the tube by frictional contact between the extension wall and the tube at the distal tube opening. At the detached position the tube extension is physically separate from the tube. The sorbent bed is disposed at an axial distance from the distal tube opening and in the tube or the tube extension. The sorbent bed has a composition configured for solid phase extraction.
    Type: Grant
    Filed: November 1, 2010
    Date of Patent: April 14, 2015
    Assignee: Agilent Technologies, Inc.
    Inventors: Robert Lee Grenz, William C. Hudson
  • Patent number: 8999729
    Abstract: A device for conducting an agglutination assay comprising several reaction vessels, each reaction vessel comprising an upper chamber having an opening for accepting reactants and/or a sample; and a lower chamber comprising an end in communication with the upper chamber for receiving fluids from the upper chamber, a closed end opposite to the end, and a matrix for separating agglutinates from non-agglutinates; wherein the device further comprises a rotating support able to rotate around an axis and holding pivotally the reaction vessels in a way to allow the reaction vessels to pivot about an axis essentially perpendicular to the rotation axis of the support when the latter is rotated, such that the fluids remain in the upper chamber when the support is not rotated, and can flow from the upper chamber to the lower chamber and into the matrix when the support is rotated.
    Type: Grant
    Filed: June 21, 2011
    Date of Patent: April 7, 2015
    Assignee: Symbion Medical Systems Sarl
    Inventors: Isabelle Delafosse, Fabrice Ummel, Antonio Yanez
  • Publication number: 20150079194
    Abstract: Systems and methods for purification and concentration of autologous alpha-2 macroglobulin (A2M) from whole blood and or recombinant A2M are provided. Also provided are methods of treating wounds with A2M. Methods for utilizing A2M in combination with other treatments (e.g., platelets and other growth factors) are provided in addition to combinations with exogenous drugs or carriers. Also provided is a method of producing recombinant A2M wild type or variants thereof where the bait region was modified to enhance the inhibition characteristics of A2M and/or to prolong the half-life of the protein for treating wounds.
    Type: Application
    Filed: August 28, 2014
    Publication date: March 19, 2015
    Inventors: Lewis HANNA, John David LAUGHLIN, Shawn Robert BROWNING
  • Patent number: 8962343
    Abstract: The invention relates to a process and a device for parallel fractionating a multiplicity of individual samples (29) within a 3-dimensional separation medium (6), with the subsequent process steps being carried out: A first space (2) which extends essentially across all three space coordinates contains the separation medium (6). A multiplicity of individual samples (29) is arranged close to an interface of the first space (2), with the individual samples (29) being arranged essentially in a planar fashion, i.e. their particular center-of-gravity positions are described by two coordinates. Under the influence of one or more physical parameters, the individual samples (29) are able to migrate essentially perpendicularly to the area of their application through the separation medium (6), and are fractionated in the process according to one or more of their properties.
    Type: Grant
    Filed: March 5, 2003
    Date of Patent: February 24, 2015
    Inventors: Robert Alexander Ventzki, Josef Stegemann
  • Patent number: 8961791
    Abstract: The present relates to a molecular exchange device. In particular, the present invention relates to a molecular exchange device comprising an outer tube extending from an approximal end to a distal end of the device; an inner tube defining at least one fluid passageway, the inner tube positioned concentrically within the outer tube and the area between the inner and the outer tube defining at least one fluid passageway; and a projection positioned within the area between the inner and the outer tube, wherein the projection prevents displacement of the inner tube with respect to the outer tube.
    Type: Grant
    Filed: February 5, 2009
    Date of Patent: February 24, 2015
    Assignee: Probe Scientific Limited
    Inventors: Mark Thomas O'Connell, Stewart Jeffrey Block, Rodney Ruston
  • Publication number: 20150051383
    Abstract: Among other things, in general, a two-stage filtration and extraction assembly is described, as well as methods of use thereof.
    Type: Application
    Filed: May 9, 2013
    Publication date: February 19, 2015
    Applicant: Dalhousie University
    Inventors: Alan A. Doucette, Mark James Wall
  • Patent number: 8940542
    Abstract: A sensor for detecting and/or quantifying the amount of analyte in a sample, the sensor including: a sensing region; and a barrier layer including a reactive oxygen species (ROS)-quenching, analyte-permeable membrane having an ROS-quenching agent adsorbed to the membrane; wherein the sensor is adapted so that the sample enters the sensing region of the sensor through the barrier layer.
    Type: Grant
    Filed: October 8, 2013
    Date of Patent: January 27, 2015
    Assignee: Lighthouse Medical Limited
    Inventors: William Paterson, Nick Barwell, Bruce Culbert, Barry Colin Crane
  • Patent number: 8932864
    Abstract: Provided is a method and device for identifying glycated protein in a sample using a matrix including a boronic acid moiety that binds glycated proteins.
    Type: Grant
    Filed: March 18, 2013
    Date of Patent: January 13, 2015
    Assignee: Samsung Electronics Co., Ltd.
    Inventors: Sang-kyu Kim, Sung-ha Park, Kyung-mi Song, Soo-suk Lee, Youn-suk Choi
  • Patent number: 8925734
    Abstract: A filter vial and piston are provided where the vial has a cylindrical wall with a closed bottom and open top and with the hollow, tubular piston therein. The piston has a distal end covered by a cup having a proximal cup seal extending outward to engage the walls of the vial to form a fluid tight seal with the vial during use. The distal end of the piston has a piston flange extending outwardly and located adjacent a recess in the piston so the cup forms a snap fit over the piston flange and extends into the recess. The piston flange causes the cup to bulge sufficiently outward to form a fluid tight seal with the vial during use. A filter is placed in the bottom of the cup and abuts an inwardly extending lip on the cup. The filter is held against the lip by a piston support at the distal end of the piston. A cup support at a distal end of the cup also helps support the filter.
    Type: Grant
    Filed: January 28, 2013
    Date of Patent: January 6, 2015
    Assignee: Scientific Plastic Products, Inc.
    Inventors: Samuel A. Ellis, Kishan G. Hingorani
  • Patent number: 8906234
    Abstract: A filter device of the present disclosure includes a first port from which a solution containing a substance is to be input, and a first flow passage communicating with the first port. A filter portion made of a plurality of fibrous substances including inorganic oxide is formed in at least one part in the first flow passage. The plurality of fibrous substances has one peak in the diameter distribution.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: December 9, 2014
    Assignee: Panasonic Corporation
    Inventors: Takeki Yamamoto, Masaya Nakatani, Takuya Oka, Takahiro Nogami
  • Patent number: 8889071
    Abstract: An apparatus for separating plasma by which plasma can be separated from a small amount of whole blood cell sample without centrifugation is disclosed. This apparatus includes a blood channel through which blood flows; and a plasma channel through which plasma separated from said blood flows. The plasma channel is arranged at least partially in parallel with said blood channel and the blood channel and the plasma channel are at least partially in contact with each other along the longitudinal direction of the channels. Blood is made to flow at a flow rate at which blood cell components in the blood flowing through the blood channel axially accumulate and at which hemolysis does not occur. The plasma moves to the plasma channel after being separated into a blood cell layer and a plasma layer.
    Type: Grant
    Filed: December 7, 2009
    Date of Patent: November 18, 2014
    Assignees: Institute of Microchemical Technology Co., Ltd., The University of Tokyo
    Inventors: Arata Aota, Takehiko Kitamori
  • Patent number: 8865074
    Abstract: A sample analysis cartridge and a sample cartridge reader are provided. In measuring a particular component included in a sample flowing in a microfluidic channel, a numerical value of hematocrit is reflected to thus improve the accuracy of measurement of the particular component.
    Type: Grant
    Filed: December 29, 2010
    Date of Patent: October 21, 2014
    Assignee: LG Electronics Inc.
    Inventors: Keumcheol Kwak, Seokjung Hyun, Taeyoon Lee, Jitae Kim, Dayeon Kang, Gueisam Lim, Yeonjae Kang
  • Patent number: 8852531
    Abstract: The present invention relates to a system for detecting microbial contamination of a liquid specimen comprising a device for concentrating micro-organisms from a liquid specimen, having (i) a hypobaric chamber, (ii) a filter housing comprising a liquid-permeable bed of an adsorbent material and adapted for being fluidly connected to said hypobaric chamber, and (iii) a vacuum pump adapted for being fluidly connected to said hypobaric chamber, said system further comprising a kit for detection of micro-organisms adsorbed to said adsorbent material, wherein said kit is based on enzymatic detection using chromogenic and/or fluorescent substrate analogs.
    Type: Grant
    Filed: November 24, 2008
    Date of Patent: October 7, 2014
    Assignee: Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek TNO
    Inventors: Jan Gerritse, Johannes Wouterus van Groenestijn, Neeltje Dina Zegers
  • Publication number: 20140286836
    Abstract: A device for sampling and transporting nano-objects for analysis thereof. A cassette portion of the device includes two parts with mutual assembly mechanisms between them including a clipping mechanism configured not to be accessible from outside in assembled position of the parts. Therefore, any inopportune opening of the cassette and thus extraction of the sampling filter accommodated in the cassette somewhere else than the place intended for the analysis are avoided.
    Type: Application
    Filed: October 5, 2012
    Publication date: September 25, 2014
    Applicants: NANOBADGE, Commissariat à l'energie atomique et aux ene alt
    Inventors: Simon Clavaguera, Eric Decolin, Guillaume Hebert, Jean-Loup Rechatin, Francois Tardif
  • Publication number: 20140248714
    Abstract: Methods and systems for processing samples fixed to a porous substrate generally comprising, a compressor defining one or more fluid isolation areas, a support, for the porous substrate, having an opening corresponding to one or more of the fluid isolation areas of the compressor, an actuator that causes at least a portion of the compressor to press against the porous substrate, a fluid inlet having access to the fluid isolation area at least when the compressor is pressed against the porous substrate, and a fluid outlet to receive fluid, through the opening in the support corresponding to the fluid isolation area of the compressor, at least when the compressor is pressed against the porous substrate.
    Type: Application
    Filed: March 10, 2014
    Publication date: September 4, 2014
    Applicant: WHATMAN INTERNATIONAL LIMITED
    Inventors: Philip Alexander Shoemaker, Weston Blaine Griffin, Erin Jean Finehout, Xuefeng Wang, Kashan Ali Shaikh, Greg Darryl Goddard
  • Patent number: 8808552
    Abstract: An apparatus and method to perform multiple filtration steps with a modular filtration apparatus and single cycle. A stackable modular filter cup has features to allow stacked cups to be separated without the need for substantial effort yet without diminishing the effect of vacuum assisted filtration. Each cup is formed with a slip seal segment and/or a series of substantially vertical channels or ridges on an inner or outer wall to reduce surface contact between nested filter cups.
    Type: Grant
    Filed: December 16, 2010
    Date of Patent: August 19, 2014
    Assignee: ZenPure (Hangzhou) Co., Ltd.
    Inventor: Zhenwu Lin
  • Publication number: 20140221239
    Abstract: System, including methods and apparatus, for spacing droplets from each other and for detection of spaced droplets.
    Type: Application
    Filed: February 3, 2014
    Publication date: August 7, 2014
    Applicant: Bio-Rad Laboratories, Inc.
    Inventors: George Carman, Thomas H. Cauley, III, David P. Stumbo
  • Publication number: 20140212343
    Abstract: Provided is a composite membrane for western blot, in which the composite membrane is prepared by combining nanofiber webs with nonwoven fabrics, and a basis weight of the nanofibers is in a range of 1 gsm to 50 gsm on the nonwoven fabrics, and an average pore size is in a range of 0.1 ?m to 1.0 ?m. The composite membrane for western blot including nanofibers has advantages such as saving of a production cost, and an excellent response characteristic due to a capillary phenomenon of a double structure, to thereby easily detect even a small amount of a particular substance present in a protein.
    Type: Application
    Filed: April 1, 2014
    Publication date: July 31, 2014
    Applicants: AMOMEDI CO., LTD., AMOGREENTECH CO., LTD.
    Inventors: Chan KIM, Eu Gene CHO, Sang Chul SUH, In Yong SEO
  • Publication number: 20140205503
    Abstract: An improved apparatus and method for dispersion of a labeling conjugate in a diagnostic assay, the result being a one-step assay. By eliminating a conjugate pad as in conventional lateral diagnostic devices, and forming a frazil ice pellicle (FIP), rehydration and flow are improved resulting in better reproducibility, improved sensitivity, and reduced costs of individual assay devices. The formation of a frazil ice film formed on a super cooled surface of a sample receiving means simplifies assay assembly. Lyophilization of the FIP improves the release of a sample/analyte/label matrix into into a macro channel as in a direct flow assay, while at the same time allowing reagents to mix and flow, thereby optimizing the assay performance. The reagents of the conjugate and the formation of the FIP stabilize the conjugate proteins and provide extended shelf life to the diagnostic assay device.
    Type: Application
    Filed: January 22, 2013
    Publication date: July 24, 2014
    Applicant: QUANTUM DESIGN INTERNATIONAL, INC.
    Inventors: Ronald T. LaBorde, Nicholas J. Neild
  • Patent number: 8758702
    Abstract: A clinical diagnostic sample analyzer for analyzing a sample of a patient is disclosed. The analyzer includes a telescoping closed-tube sampling assembly with a sample probe concentrically housed within a piercing probe and a venting mechanism. The closed-tube sampling assembly is used for aspirating a sample from a sample tube for analysis by a clinical diagnostic sample analyzer.
    Type: Grant
    Filed: May 4, 2006
    Date of Patent: June 24, 2014
    Assignee: Instrumentation Laboratory Company
    Inventors: Matthew Blouin, Gregory Murphy, Diana Mackenzie, Alan Weeks, Daniel Kobrenski
  • Patent number: 8747669
    Abstract: A separation module and method are disclosed for processing a liquid sample and providing high conversion by operating a single-pass tangential-flow process without a recirculation loop. In one embodiment, the separation module includes three reservoirs and has at least one long, thin channel with a large ratio of channel membrane area to: channel void volume; volume of a sample feed reservoir; and volume of the feed sample. In another embodiment, the separation module includes an external feed reservoir, an external retentate reservoir fluidly and a first vacuum source adapted to apply a first vacuum to the separation element disposed within the housing. The single-pass TFF separation element and single-pass process provides high conversion while operating with relatively low pressure sources. Certain embodiments disclosed herein can be used for the separation of blood.
    Type: Grant
    Filed: August 15, 2010
    Date of Patent: June 10, 2014
    Assignee: SPF Innovations, LLC
    Inventors: Alex G. Bonner, Gaston de los Reyes
  • Patent number: 8728312
    Abstract: Disclosed are a method and an apparatus for filtering plasma using magnetic force. The apparatus for filtering plasma using magnetic force includes: an inlet into which blood is injected; a filter unit filtering plasma in the blood passing through the inlet by capillary force; a magnetic force receiving part made of magnetizable materials and assisting plasma filtering by applying pressure to the filter unit by movement due to magnetic force generated from the outside; an outlet discharging plasma filtered from blood; and a filter outer body surrounding the inlet, the filter unit, the magnetic receiving part, and the outlet.
    Type: Grant
    Filed: October 31, 2011
    Date of Patent: May 20, 2014
    Assignee: Electronics and Telecommunications Research Institute
    Inventors: Kwang Hyo Chung, Yo Han Choi, Chang-Geun Ahn, Gun Yong Sung
  • Publication number: 20140134718
    Abstract: The present invention relates to a device for rapid isolation of target molecules from cell lysates and other liquid mixtures comprising particulate material, a method for isolating the target molecules, in particular nucleic acids, using said device and a kit for carrying out said method comprising said device.
    Type: Application
    Filed: June 29, 2012
    Publication date: May 15, 2014
    Applicant: QIAGEN GmbH
    Inventors: Margit Hiesinger, Karin Schulte
  • Patent number: 8722416
    Abstract: An analytical method comprising performing a first fractionation of a polymer sample based on differences in crystallizability to provide a first set of sample fractions, performing a first analysis on the first set of sample fractions, performing a second fractionation of the first set of sample fractions to produce a second set of sample fractions, performing a second analysis on the second set of sample fractions, and synchronizing the first fractionation and second fractionation to provide about concurrent analysis of the polymer sample.
    Type: Grant
    Filed: May 24, 2006
    Date of Patent: May 13, 2014
    Assignee: Chevron Phillips Chemical Company LP
    Inventors: Youlu Yu, Chung C. Tso, Paul J. DesLauriers
  • Patent number: 8715593
    Abstract: The present invention is a pipette tip device for extraction of liquid, semi-solid or solid solutions to be chemically analyzed and the methods for their use. The pipette tip extraction device contains a screen or filter at its lower narrow end to contain solid particulate matter and a barrier at its upper wide end. The optional upper frit is to be made of material that permits liquid solutions to flow through it. In addition to the barrier and screen, the pipette tip extraction device may contain solid-phase sorbent. Through the use of a removable cap, the pipette extraction tip may also serve as a sample collection container or tip in which samples can be delivered to the top of the tip (e.g., for direct collection of samples, including solid samples). A new method for DPX extraction using liquid-liquid-solid-phase extraction is also disclosed.
    Type: Grant
    Filed: February 21, 2008
    Date of Patent: May 6, 2014
    Inventor: William Brewer
  • Patent number: 8697009
    Abstract: The present invention relates to microfluidic devices and methods for manipulating and analyzing fluid samples. The disclosed microfluidic devices utilize a plurality of microfluidic channels, inlets, valves, filter, pumps, liquid barriers and other elements arranged in various configurations to manipulate the flow of a fluid sample in order to prepare such sample for analysis.
    Type: Grant
    Filed: September 6, 2013
    Date of Patent: April 15, 2014
    Assignee: Micronics, Inc.
    Inventors: Patrick Saltsman, Mingchao Shen, Jeffrey M. Houkal, Christy A. Lancaster, C. Frederick Battrell, Bernhard H. Weigl
  • Publication number: 20140099241
    Abstract: Disclosed herein are methods of making micropores of a desired height and/or width between two isotropic wet etched features in a substrate which comprises single-level isotropic wet etching the two features using an etchant and a mask distance that is less than 2× a set etch depth. Also disclosed herein are methods using the micropores and microfluidic devices comprising the micropores.
    Type: Application
    Filed: October 18, 2013
    Publication date: April 10, 2014
    Applicant: Sandia Corporation
    Inventors: Thomas D. Perroud, Kamlesh D. Patel, Robert J. Meagher
  • Patent number: 8679857
    Abstract: The invention provides pipette tip extraction columns for the purification of a DNA vector from un-clarified cell lysate containing cell debris as well as methods for making and using such columns. The columns typically include a bed of extraction media positioned in the pipette tip column, above a bottom frit and with an optional top frit.
    Type: Grant
    Filed: January 28, 2013
    Date of Patent: March 25, 2014
    Assignee: PhyNexus, Inc.
    Inventors: Chris Suh, Lee Hoang, Jennifer E. Grant, Douglas T. Gjerde
  • Patent number: 8673239
    Abstract: A sample collector and test device is disclosed. The sample collector and test device may be used together as a diagnostic tool for collecting and assay of analytes contained in a sample. A sample collector is also disclosed which may be used with existing sample containers and or test devices. The sample collector may indicate sample adequacy when a sufficient volume of sample has been collected for assay and may also include a sample retaining feature which retains a portion of expressed sample for confirmatory testing, if desirable. The sample collector may also include a mechanism for expressing sample in a sample collector and/or test device. A test device is also disclosed for retaining and assay of an expressed sample. The disclosed test device may be used with existing sample collectors. In a preferred embodiment, test device is used with a preferred sample collector.
    Type: Grant
    Filed: January 31, 2011
    Date of Patent: March 18, 2014
    Assignee: Orasure Technologies, Inc.
    Inventors: R. Sam Niedbala, John W. Scott, Peter A. Bourdelle, Hans H. Feindt
  • Patent number: 8663580
    Abstract: A dried biological fluid spot punch device includes a tube, a frit, and a filter disposed on the frit. The tube includes a main section and a distal section adjoining the main section. The main section includes a proximal tube end circumscribing a proximal tube opening, and the distal section includes a distal tube end circumscribing a distal tube opening. The distal section further includes a distal tube having a tapered inside diameter that reduces from the main section to the distal tube opening. The frit is disposed in the distal section at a distance from the distal tube opening and fixed in position by frictional contact with the distal tube wall. The punch device may also include a punching tool. The punching tool may include a body engaging the tube at the proximal tube end and an ejection mechanism configured for disengaging the body from the tube.
    Type: Grant
    Filed: November 1, 2010
    Date of Patent: March 4, 2014
    Assignee: Agilent Technologies, Inc.
    Inventors: Robert Lee Grenz, William C. Hudson
  • Patent number: 8652422
    Abstract: The invention is directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips and other such devices is preferred in doing so.
    Type: Grant
    Filed: April 1, 2011
    Date of Patent: February 18, 2014
    Assignee: EMD Mllipore Corporation
    Inventors: Masaharu Mabuchi, Hiroko Kimura, Marc Emerick, Phillip Clark, Kurt Greenizen
  • Patent number: 8652421
    Abstract: The invention is directed to an apparatus useful in conducting detection of compounds on blotting membranes. The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and optionally a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane and the support layer is a grid or sintered porous material. The distributor and support are held together to form an envelope around the membrane(s). The use of a hinge, clips and other such devices is preferred in doing so.
    Type: Grant
    Filed: October 18, 2006
    Date of Patent: February 18, 2014
    Assignee: EMD Millipore Corporation
    Inventors: Masaharu Mabuchi, Hiroko Kimura, Marc Emerick, Phillip Clark, Kurt Greenizen
  • Patent number: 8623296
    Abstract: Microfluidic devices, assemblies, and systems are provided, as are methods of manipulating micro-sized samples of fluids. Microfluidic devices having a plurality of specialized processing features are also provided.
    Type: Grant
    Filed: August 4, 2010
    Date of Patent: January 7, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: Sean M. Desmond, Zbigniew T. Bryning, John Shigeura, Gary Lim, Adrian Fawcett, Jacob K. Freudenthal, Gary Bordenkircher
  • Publication number: 20140004021
    Abstract: Systems and methods for treating a fluid with a body are disclosed. Various aspects involve treating a fluid with a porous body. In select embodiments, a body comprises ash particles, and the ash particles used to form the body may be selected based on their providing one or more desired properties for a given treatment. Various bodies provide for the reaction and/or removal of a substance in a fluid, often using a porous body comprised of ash particles. Computer-operable methods for matching a source material to an application are disclosed. Certain aspects feature a porous body comprised of ash particles, the ash particles have a particle size distribution and interparticle connectivity that creates a plurality of pores having a pore size distribution and pore connectivity, and the pore size distribution and pore connectivity are such that a first fluid may substantially penetrate the pores.
    Type: Application
    Filed: December 25, 2012
    Publication date: January 2, 2014
    Inventors: Charles E. Ramberg, Stephen A. Dynan, Jack A. Shindle
  • Patent number: 8617897
    Abstract: Methods and kits to detect and quantitate NOx compounds in a biological sample are provided. The methods include reacting a mixture that includes a nitrite compound and a chromogenic reagent to form a colored compound, contacting the mixture with a retention medium configured to retain the colored compound, and detecting the colored compound retained on the retention medium.
    Type: Grant
    Filed: August 3, 2010
    Date of Patent: December 31, 2013
    Assignee: 3M Innovative Properties Company
    Inventor: Vinod P. Menon
  • Patent number: 8617904
    Abstract: A method of processing animal sperm cells that includes: collecting sperm cells from a male animal; sorting the sperm cells to obtain a quantity of sperm cells having a desired characteristic, the quantity of sorted sperm cells being contained in a first volume of fluid having a first concentration of sperm cells; and subjecting the sperm cells to concentration steps in which the concentration of the sperm cells is increased to a second concentration greater than the first concentration.
    Type: Grant
    Filed: February 25, 2013
    Date of Patent: December 31, 2013
    Assignee: Inguran, LLC
    Inventors: Gary Durack, Muhammad Anzar, Cindy Ludwig, Jeffrey A. Graham, Kathleen S. Crowley, Bradley Didion
  • Patent number: 8603395
    Abstract: A self-propelled apparatus for analyzing a component contained in a fluid medium. The self-propelled apparatus uses kinetic energy of the apparatus to drive a fluid under analysis through the apparatus. This is accomplished by use of a conveyance system that is attached to the analytical system of the apparatus. A sensor system is used to analyze the component collected within the confines of an analysis chamber, a part of the analysis system. The invention also includes a method of using the analytical apparatus.
    Type: Grant
    Filed: November 10, 2009
    Date of Patent: December 10, 2013
    Assignee: University of South Florida
    Inventors: David P Fries, Michelle L Janowiak, George Steimle, Heather A Broadbent
  • Patent number: 8562840
    Abstract: A spin column device, which contains a rigid porous filter that retains its shape during centrifugation, chromatography methods using the device to isolate a desired substance, e.g., a biological molecule, from other substances in a mixture, and kits containing the device with one or more reagents for use in the method.
    Type: Grant
    Filed: May 2, 2012
    Date of Patent: October 22, 2013
    Assignee: Bioventures, Inc.
    Inventor: Elliott P. Dawson
  • Patent number: 8557183
    Abstract: A submersible, self-propelled apparatus for analyzing a component contained in a liquid medium. The submersible, self-propelled apparatus uses kinetic energy of the apparatus to drive a liquid under analysis through the apparatus. This is accomplished by use of a conveyance system that is attached to the analytical system of the apparatus. A sensor system is used to analyze the component collected within the confines of an analysis chamber, a part of the analysis system. The invention also includes a method of using the analytical apparatus.
    Type: Grant
    Filed: November 10, 2009
    Date of Patent: October 15, 2013
    Assignee: University of South Florida
    Inventors: David P Fries, Michelle L Janowiak, George Steimle, Heather A Broadbent
  • Patent number: 8540078
    Abstract: Extracting or removing at least a portion of liquid phase from a whole sample using a centrifugal force is disclosed. Centrifugal forces are used to apply pressure to a whole sample and drive a liquid phase through a passage region that can be perforated and/or porous and maintain a drier portion within a separation container. The whole sample can be dried, which includes a remaining sample where excess or a selected amount of liquid is removed. Direct access to the separation container or area can then be made to provide for an efficient withdrawal of the drier material from the separation container.
    Type: Grant
    Filed: April 12, 2010
    Date of Patent: September 24, 2013
    Assignee: Biomet Biologics, LLC
    Inventors: Michael D. Leach, Jason Chavarria