Analyzer, Structured Indicator, Or Manipulative Laboratory Device Patents (Class 422/50)
  • Patent number: 10809254
    Abstract: Provided herein are methods of splitting droplets containing magnetically responsive beads in a droplet actuator. A droplet actuator having a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at the droplet operations electrodes, is provided. The magnetically responsive beads in the droplet are immobilized using the magnetic field and the plurality of droplet operations electrodes are used to split the droplet into first and second droplets while the magnetically responsive beads remain substantially immobilized.
    Type: Grant
    Filed: November 14, 2018
    Date of Patent: October 20, 2020
    Inventors: Vamsee K. Pamula, Ramakrishna Sista, Vijay Srinivasan, Michael G. Pollack, Allen E. Eckhardt
  • Patent number: 10775284
    Abstract: There is provided a method of charging an array of micro-capillaries. The micro-capillaries have at least one end that is open for fluid communication. The method includes the steps of: (a) filling the array of micro-capillaries with an assay liquid; (b) controllably evaporating at least some of the assay liquid to remove it from the micro-capillary and create a void space in each of the capillaries between the assay liquid and the open end; and (c) filling the void space with a liquid that is immiscible with said assay liquid. There is also provided a use of the disclosed method and a device for charging an array of micro-capillaries.
    Type: Grant
    Filed: May 26, 2016
    Date of Patent: September 15, 2020
    Assignee: JN Medsys Pte Ltd
    Inventors: Kian Kok Johnson Ng, Koon Kiat Teu, Mei Tze Belinda Ling, Jia Wen Sim
  • Patent number: 10717881
    Abstract: An electrical device capable of water-resisting, vapor resistant and electrically conducting in a moisture-containing environment and a method for making such electrical device are provided. The present invention for use in electrically conductive aqueous solutions enables the electrical device to be electrically conductive in any direction because of quantum tunneling effect. The electrical device includes a device body having at least one surface, and a plurality of nano-particles embedded in one of surface pores of the at least one surface of the device body. The particle size of the nano-particles is less than tens of nanometers.
    Type: Grant
    Filed: November 30, 2016
    Date of Patent: July 21, 2020
    Assignee: Dazzeon Technology Co., LTD
    Inventor: Thomas Chen
  • Patent number: 10688491
    Abstract: According to the invention, generally, a method for making a microfluidic aliquoting (MA) chip, adapted to fit in a Petri dish, has a center well (inlet) connected by branched channels to a plurality of side wells (outlets). The chip comes in various types, including a bMA Chip T1, bMA Chip T2, bMA Chip T3, and an rMA Chip. The branched channel improvement provides for a greater distance between neighboring channels and a decreased density near the center well. Design improvements including an injection mold design for an insert and a base and a multiplex hole punch allow for rapid fabrication of the MA chip.
    Type: Grant
    Filed: November 24, 2017
    Date of Patent: June 23, 2020
    Inventor: Lidong Qin
  • Patent number: 10654040
    Abstract: Embodiments are directed to a platform for liquid droplet generation and isolation for biochemical sensing and testing. An embodiment includes generator, fluid-exchange, and manipulator structures that are vertically aligned on a substrate to form a collection chamber. The generator structure is configured to form liquid droplets from a stream of liquid using gas. The fluid-exchange structure is connected to the generator structure to receive the liquid droplets in a carrier liquid held in the collection chamber. The manipulator structure is connected to receive the liquid droplets in the carrier liquid via an inlet. The manipulator structure defines a manipulator chamber connected to the inlet and has a first outlet and a second outlet and a filter capable of filtering the liquid droplets from the carrier liquid. The first outlet enables removal of the liquid droplets filtered and the second outlet enables removal of the carrier liquid.
    Type: Grant
    Filed: August 18, 2017
    Date of Patent: May 19, 2020
    Assignee: Northeastern University
    Inventors: Pooyan Tirandazi Khalilabad, Carlos H. Hidrovo Chaves
  • Patent number: 10646864
    Abstract: The purpose of the present invention is to provide a detection method which can safely recover a remaining sample from a detection device without causing a biological disaster. In order to achieve at least one of these goals, this detection method involves: a sample collection step for collecting a sample from the sample holding well, and transferring the same to a detection chip; a detection step for detecting the detected substance captured in the detection chip by means of a detection unit; and, after the sample collection step, a remaining sample recovery step for collecting the sample remaining in the sample holding well and transferring this to a sample recovery well. At that time, after the remaining sample recovery step, the surface area of the opening in the sample recovery well is smaller than the surface area of the opening in the sample holding well.
    Type: Grant
    Filed: January 29, 2016
    Date of Patent: May 12, 2020
    Assignee: Konica Minolta, Inc.
    Inventors: Youichi Aoki, Masataka Matsuo, Tetsuya Noda
  • Patent number: 10646872
    Abstract: A support for conserving samples of biological material comprising at least: a first portion of an absorbent material aimed at and destined to conserve a sample of biological material and a second portion, distinct from the first portion and aimed at, predisposed and destined to constitute a cleaning zone for a head of a device, in particular a punch, aimed at collecting a sample of biological material from the first portion. The support can further comprise a third portion of connection interposed between the first portion and the second portion; the third portion can exhibit one or more cuts or one or more openings.
    Type: Grant
    Filed: June 23, 2014
    Date of Patent: May 12, 2020
    Assignee: Copan Italia S.p.A.
    Inventor: Daniele Triva
  • Patent number: 10646899
    Abstract: According to one embodiment, a cell sorter includes a flow channel which supplies a sample liquid containing particles, a plurality of branch channels connected to the flow channel, an image sensor which has a pixel region covering the flow channel and the branch channels, a determination unit which determines the characteristics of the particles in the sample liquid from a measurement signal of the pixel region, and a separation unit guides the particles in the sample liquid to any of the branch channels based on the determination result of the determination unit.
    Type: Grant
    Filed: March 8, 2018
    Date of Patent: May 12, 2020
    Inventors: Kaita Imai, Shouhei Kousai, Yosuke Akimoto, Michihiko Nishigaki, Yutaka Onozuka, Miyu Nagai
  • Patent number: 10627390
    Abstract: A method for analyzing a biologic fluid sample includes the steps of: a) providing a spatially mapped chamber; b) providing a predetermined repeatable non-uniform spatial distribution of one or more constituents within the sample, which distribution indicates the presence or absence of a statistically significant number of constituents within the sample in each chamber sub-region; c) selecting one or more image techniques for each sub-region based on the presence or absence of the statistically significant number of one or more constituents in that sub-region as indicated by the distribution; d) creating image data representative of the biologic fluid sample in each sub-region, using the one or more image techniques selected for that sub-region; and e) analyzing the sample.
    Type: Grant
    Filed: August 13, 2018
    Date of Patent: April 21, 2020
    Assignee: Abbott Point of Care, Inc.
    Inventors: Min Xie, Stephen C. Wardlaw
  • Patent number: 10549480
    Abstract: The invention is about an ultrasonic welding-based microfluidic device. It is mainly made of a first element and a second element welded one to the other via at least one structure (10, 10?). The structure (10, 10?) comprises an elongated welded portion for said welding, a welding channel (12, 12?) extending between the first and second elements and along one side of the welded portion, and a draining channel (13) communicating with the welding channel (12, 12?) and the microfluidic path (20, 20?) of the device. The invention is further about a method of manufacturing such a device.
    Type: Grant
    Filed: November 17, 2017
    Date of Patent: February 4, 2020
    Inventors: Niels Agersnap Larsen, Klaus Mogensen
  • Patent number: 10544039
    Abstract: Methods for depositing a measured amount of a species in a sealed cavity. In one example, a method for depositing molecules in a sealed cavity includes depositing a selected number of microcapsules in a cavity. Each of the microcapsules contains a predetermined amount of a first fluid. The cavity is sealed after the microcapsules are deposited. After the cavity is sealed the microcapsules are ruptured to release molecules of the first fluid into the cavity.
    Type: Grant
    Filed: September 8, 2017
    Date of Patent: January 28, 2020
    Inventors: Benjamin Stassen Cook, Kurt Wachtler, Adam Joseph Fruehling, Juan Alejandro Herbsommer, Simon Joshua Jacobs
  • Patent number: 10539560
    Abstract: A substrate for sample analysis is a substrate for sample analysis used for causing a binding reaction between an analyte and a ligand in a liquid sample, the substrate for sample analysis including: a base substrate having a rotation axis and a predetermined thickness; a chamber located in the base substrate for holding a liquid sample containing an analyte and a ligand immobilized on a surface of magnetic particles; and at least one magnet arranged at a position such that the magnetic particles are captured in the chamber by the magnet.
    Type: Grant
    Filed: June 29, 2015
    Date of Patent: January 21, 2020
    Inventors: Masayuki Saiki, Masahiro Johno, Fusatoshi Okamoto, Kazuya Kondoh
  • Patent number: 10525461
    Abstract: The present disclosure provides a cartridge for purifying biological samples. The cartridge comprises a first chamber configured to receive and hold biological samples, and at least one second chamber configured to receive and hold a reagent solution. A first fluid flow control valve is fluidly connected to outlet ports of the first chamber and the at least one second chamber. Also, the first fluid flow control valve is fluidly connected to a matrix chamber and is configured to selectively route biological samples and reagent solution into the matrix chamber for purifying the biological samples. A binding matrix is configured in the matrix chamber for capturing the nucleic acids from the biological samples and the reagent solution. Further, a elute collection chamber is fluidly connected to the matrix chamber, wherein the elute collection chamber is configured to receive and hold purified biological sample from the matrix chamber.
    Type: Grant
    Filed: August 30, 2016
    Date of Patent: January 7, 2020
    Assignee: Bigtec Private Limited
    Inventors: Manoj Mulakkapurath Narayanan, Chandrasekhar Bhaskaran Nair
  • Patent number: 10520472
    Abstract: A novel SH-SAW biosensor capable of non-invasive and touch-free detection of cancer cell viability and growth or proliferation in two-dimensional (2D) and three-dimensional (3D) cell cultures as well as stem cell regeneration as it pertains to cancer cell biology and anti-cancer drug development is presented. The biosensor includes two pairs of resonators including interdigital transducers reflecting fingers to quantify mass loading by the cells in suspension as well as within a tumoroid culture platform. The biosensor can be part of a perfused 3PNS-tumoroid system that is amenable to real-time non-invasive monitoring of the cell proliferation, viability, and multiplexed detection of key physiologic and clinical biomarkers.
    Type: Grant
    Filed: August 21, 2017
    Date of Patent: December 31, 2019
    Assignees: University of South Florida, TransGenex Nanobiotech, Inc.
    Inventors: Shyam S. Mohapatra, Subhra Mohapatra, Rasim Oytun Guldiken, Rajesh R. Nair, Tao Wang
  • Patent number: 10509007
    Abstract: Interference signals from other gases in an air sample are eliminated by flowing an air sample directly to an electrochemical gas sensor and subsequently flowing an air sample through a selective scrubber and removing the target gas of interest before flowing the sample to the electrochemical gas sensor. The difference in responses of the electrochemical gas sensor is directly proportional to the concentration of the target gas of interest in the air sample.
    Type: Grant
    Filed: December 18, 2015
    Date of Patent: December 17, 2019
    Assignee: Aeroqual Limited
    Inventor: Geoffrey Stephen Henshaw
  • Patent number: 10501795
    Abstract: A probe system comprising (a) a first single-stranded oligonucleotide labelled with detectable elements in an undetectable state, and (b) second and third single-stranded oligonucleotides capable of hybridising to complementary regions on the first oligonucleotide.
    Type: Grant
    Filed: November 9, 2017
    Date of Patent: December 10, 2019
    Inventors: Barnaby Balmforth, Cameron Alexander Frayling
  • Patent number: 10495637
    Abstract: A universal sensor fabrication approach, molecular substrate imprinting technique, which utilizes the interaction between molecular building blocks and the surface of a transducer to develop specific molecular recognition cavities has been established. Integration of molecular recognition cavities with the surface of a nanoscale transducer will result in a nano-tunneling effect that takes place which will provide a sensor or a device that exhibits new properties not already exhibited by either the molecular recognition cavities on a bulk transducer or the nanotransducer material. One of the new properties of this nano-tunneling effect is that a universal potentiometric molecular sensor can be fabricated and used to detect any compounds, whether they are ions or molecules, with enhanced selectivity, sensitivity, and stability when molecular recognition cavities or elements are integrated on the surface of a nanoscale transducer.
    Type: Grant
    Filed: May 6, 2015
    Date of Patent: December 3, 2019
    Inventor: Yanxiu Zhou
  • Patent number: 10493445
    Abstract: The invention relates to a fluidic system comprising at least one bead chamber (311) containing a lyophilized reagent (LB) and a reaction chamber in a cartridge. In one embodiment, a series of bead chambers with different lyophilized reagents may be provided such that sample fluid can sequentially pass through them. In another embodiment, bead chambers may be located on a movable carrier, for example a rotating carousel, from which they may selectively be connected to a reaction chamber in a cartridge. In still another embodiment, the bead chamber (311) may comprise at least one flexible wall (FW) allowing for a minimization of dead volume associated with the extraction of lyophilized reagent (LB).
    Type: Grant
    Filed: April 29, 2014
    Date of Patent: December 3, 2019
    Assignee: Koninklijke Philips N.V.
    Inventors: Anke Pierik, Reinhold Wimburger-Friedl, Johannes Theodorus Wilhelmus Maria Van Eemeren, Irene Johanna Monia Dobbelaer-Bosboom, Martinus Johannes Van Zelst, Bernadet Dagmar Marielle Meijering
  • Patent number: 10457907
    Abstract: An electrochemical measurement device includes: a base; a specimen placement portion disposed on the base, for placing a biological specimen; a first electrode disposed on the base and surrounding the specimen placement portion; and a first insulating layer covering the first electrode. The first insulating layer has a plurality of openings, and the first electrode has a plurality of first electrode exposed portions which are portions of the first electrode that are exposed from the openings of the first insulating layer.
    Type: Grant
    Filed: September 27, 2016
    Date of Patent: October 29, 2019
    Inventors: Atsushi Shunori, Masahiro Yasumi, Makoto Takahashi
  • Patent number: 10444184
    Abstract: An assembly is provided for interfacing with a microfluidic chip having at least one microscopic channel configured to receive a liquid sample for analysis. The assembly includes a chip carrier, an electronics module, an optical module, and a mechanical module. The chip carrier includes a base and a cover defining a cavity to receive the microfluidic chip. The electronics module includes a signal generator which applies at least one electrokinetic signal electrode(s) of the chip. The optical module includes an excitation radiation source which causes excitation radiation to impinge on the sample, and an emission radiation detector which detects radiation emitted from the sample. The mechanical module includes a chip-carrier receiving structure, relatable with respect to the optical module for focus and at least one degree of translational freedom.
    Type: Grant
    Filed: June 30, 2016
    Date of Patent: October 15, 2019
    Assignee: International Business Machines Corporation
    Inventors: Jaione T. Azpiroz, Emmanuel Delamarche, Claudius Feger, Ricardo L. Ohta, Mathias B. Steiner, Yuksel Temiz
  • Patent number: 10434206
    Abstract: A closure for a medical sterile container having a trough-like first container part and a lid-type second container part includes a closure flap which can be arranged on one of the container parts so as to be able to pivot between a closed position, in which the container parts are interlocked with each other by the closure, and an open position in which the container parts are unlocked. The closure includes a latch element which in the closed position of the closure interlocks the closure flap with the other container part.
    Type: Grant
    Filed: November 27, 2015
    Date of Patent: October 8, 2019
    Assignee: Aesculap AG
    Inventors: Stefan Thomas, Serkan Bellikli, Matthias Schweizer, Stefan Schuster, Dieter Weisshaupt
  • Patent number: 10422783
    Abstract: Apparatus includes a pivotable, cradling framework that can hold a rotatable bomb instrument, which can include a hollow cylinder and a grease rack. This may be employed to effect a significantly improved version of the ASTM D942 Test Method. The same is useful in measurement of grease oxidation.
    Type: Grant
    Filed: October 17, 2018
    Date of Patent: September 24, 2019
    Assignee: TANNAS COMPANY
    Inventors: Theodore W. Selby, Jonathan C. Evans, Gregory C. Miiller, Calvin David Hugo, Sarah R. Nuss-Warren, Douglas J. Wirsing
  • Patent number: 10379102
    Abstract: A single molecule sensing or detecting device includes a first electrode and a second electrode separated from the first electrode by a gap. The first electrode and the second electrode have an opening formed therethrough. At least one of the first electrode and the second electrode is functionalized with a recognition molecule. The recognition molecule has an effective length L1 and is configured to selectively bind to a target molecule having an effective length L2. The size of the gap is configured to be greater than L2, but less than or equal to the sum of L1 and L2.
    Type: Grant
    Filed: December 12, 2016
    Date of Patent: August 13, 2019
    Assignee: Arizona Board of Regents on behalf of Arizona State University
    Inventors: Stuart Lindsay, Peiming Zhang, Yanan Zhao
  • Patent number: 10371688
    Abstract: A sensing system for explosives is provided. The sensor is based on a layered structure of approximately a monolayer of a fluorophore deposited onto a few nm of a transparent polymer, supported by a substrate. The fluorophores can be xanthene laser dyes, which have high quantum yields, and the polymers can be commodity materials polymethylmethacrylate and polyvinylidene difluoride. The different fluorophore/polymer combinations give different emission responses to analytes, including both signal quenching and enhancement. The pattern of responses can be used to identify the analyte. The common explosives TNT, PETN, RDX, HMX, and TATP as gas phase species can all be uniquely identified at room temperature using only the natural vapor pressure of the explosive to deliver sample to the sensor.
    Type: Grant
    Filed: February 10, 2017
    Date of Patent: August 6, 2019
    Inventor: William B. Euler
  • Patent number: 10292586
    Abstract: The present invention relates to a non-invasive health indicator monitoring system including a sensing module, an electric power storage module, and a circuit module to collect health indicator information by contacting with a subject. In addition, the present invention also relates to a method for monitoring health indicator continuously by using the health indicator monitoring system.
    Type: Grant
    Filed: November 11, 2014
    Date of Patent: May 21, 2019
    Inventors: Yong Won Song, Su Youn Lee, Ji Yeon Lee, Jung Ah Lim, Ji Won Choi, Byung Ki Cheong, Jin Seok Kim, Ho Seong Jang, Hyun Jung Yi
  • Patent number: 10279350
    Abstract: Methods of generating droplets. In an exemplary method, a device including a sample well, a carrier well, a droplet well, and a plurality of microfluidic channels is selected. The microfluidic channels include a first channel, a second channel, and a third channel. A discrete volume of sample-containing fluid is placed into the sample well, and a discrete volume of carrier fluid is placed into the carrier well. A pressure differential is created after placing the discrete volumes, to cause fluid flow. Sample-containing fluid flows from the sample well to a droplet-generation region of the device via the first channel. Carrier fluid flows from the carrier well to the droplet-generation region via the second channel. Sample-containing droplets and carrier fluid flow from the droplet-generation region to the droplet well via the third channel.
    Type: Grant
    Filed: November 30, 2016
    Date of Patent: May 7, 2019
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Benjamin J. Hindson, Kevin D. Ness, Billy W. Colston, Jr., Fred P. Milanovich, Donald A. Masquelier
  • Patent number: 10279343
    Abstract: A transfer device for the contamination free transfer of biological material from a sealed chamber to a recipient. The transfer device includes a support having a first docking area and a second docking area. The first docking area has at least one inlet port, each inlet port creating a fluid connection with one sealed chamber. The second docking area has at least one outlet port, each outlet port creating a fluid connection with a recipient. The first docking area further includes a piercing element designed for piercing a sealing element of a sealed chamber in order to allow, together with at least one inlet port, a fluid connection between a pierced sealed chamber and the inlet port, to allow a contamination free transfer of the biological material from the pierced sealed chamber to at least one recipient.
    Type: Grant
    Filed: October 24, 2014
    Date of Patent: May 7, 2019
    Assignee: Biocartis NV
    Inventors: Nicolas Vergauwe, Thierry Tollenboom
  • Patent number: 10261012
    Abstract: This disclosure concerns methods and apparatus for interferometric spectroscopic measurements of particles with higher signal to noise ratio utilizing an infrared light beam that is split into two beams. At least one beam may be directed through a measurement volume containing a sample including a medium. The two beams may then be recombined and measured by a detector. The phase differential between the two beams may be selected to provide destructive interference when no particle is present in the measurement volume. A sample including medium with a particle is introduced to the measurement volume and the detected change resulting from at least one of resonant mid-infrared absorption, non-resonant mid-infrared absorption, and scattering by the particle may be used to determine a property of the particle. A wide range of properties of particles may be determined, wherein the particles may include living cells.
    Type: Grant
    Filed: June 6, 2016
    Date of Patent: April 16, 2019
    Assignee: 1087 Systems, Inc.
    Inventor: Matthias Wagner
  • Patent number: 10260168
    Abstract: Contemplated microfluidic devices and methods are drawn to protein arrays in which distinct and detergent-containing antigen preparations are deposited onto an optical contrast layer in a non-specific and non-covalent manner. Detection of binding a is carried out using a dye that precipitates or agglomerates to so form a visually detectable signal at a dynamic range of at least three orders of magnitude.
    Type: Grant
    Filed: February 20, 2012
    Date of Patent: April 16, 2019
    Inventors: Abraham Lee, Philip Felgner, Armando Tovar, Uland Liao
  • Patent number: 10252266
    Abstract: In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.
    Type: Grant
    Filed: July 3, 2018
    Date of Patent: April 9, 2019
    Inventors: Ju-Sung Hung, Andrew Zayac, Megan Dueck
  • Patent number: 10227634
    Abstract: The present disclosure relates generally to the integration of isotachophoresis (ITP) and isothermal nucleic acid amplification methods such as recombinase polymerase amplification (RPA). One aspect of the disclosure relates to a method for concentrating and amplifying a nucleic acid, the method including an isotachophoresis device, the isotachophoresis device including a porous matrix, and first and second electrodes, having a leading electrolyte, a trailing electrolyte and a set of isothermal nucleic acid amplification reaction reagents disposed in the porous matrix as described herein, and applying a voltage across the first electrode and the second electrode for a time sufficient to provide a first isotachophoresis (ITP) plug comprising an amplification product of the nucleic acid, wherein the concentration of the nucleic acid is substantially higher in the first FTP plug than in the first and/or second fluids outside of the first ITP plug.
    Type: Grant
    Filed: April 14, 2016
    Date of Patent: March 12, 2019
    Assignee: University of Washington
    Inventors: Jonathan D. Posner, Mark D. Borysiak, Kevin Kimura
  • Patent number: 10229564
    Abstract: An apparatus provides tactile stimulus to a human user using a tri-layer actuator comprising: first and second polymer layers which are electronically and ionically conductive and have one or more dimensions which vary in dependence on insertion of ions therein and withdrawal of ions therefrom; and a deformable layer located between the first and second polymer layers to physically separate the first and second polymer layers from one another, the deformable layer electronically insulating and ionically conductive. Application of first and second voltages between the first and second polymer layers creates corresponding first and second distributions of ions within the first and second polymer layers. Changing between application of the first and second voltages causes corresponding deformation of the tri-layer actuator between corresponding first and second shapes, a difference or transition between the first and second shapes detectable by a sense of touch of a human user.
    Type: Grant
    Filed: August 31, 2017
    Date of Patent: March 12, 2019
    Assignee: The University of British Columbia
    Inventors: Saeedeh Ebrahimi Takalloo, John D. W. Madden, Shahriar Mirabbasi, Meisam Farajollahi
  • Patent number: 10222254
    Abstract: A sample analyzing apparatus for performing an optical-based measurement on a sample includes a housing, a first light source, excitation optics, a first light detector, emission optics, and a monitoring system, all of which are disposed in the housing. The monitoring system is configured for monitoring a movable component disposed in the housing. The monitoring system includes one or more light sources for illuminating the movable component, and one or more light detectors for detecting light reflected from the movable component in response to being illuminated.
    Type: Grant
    Filed: November 7, 2016
    Date of Patent: March 5, 2019
    Assignee: Molecular Devices (Austria) GmbH
    Inventors: Michael Katzlinger, Georg Kronberger, Bernhard Schinwald
  • Patent number: 10208334
    Abstract: Systems, methods and compositions of matter according to the present invention, can be used in capture/enrichment, gene expression profiling and targeted sequencing. Provided are systems, methods and compositions concerning the enhancement of nucleic acid hybridization specificity and controlling the shapes of melting curves revealed by nucleic acid hybrid pairs to optimize nucleic acid analysis. These systems, methods and compositions comprise producing a positively charged surface or surface coating, on the surface of microarray slides or other types of surfaces similarly purposed, which enhances melting curve analysis to the point of allowing detection or differentiation of small changes in sequences between nucleic acid binding partners. The accuracy or resolution of melting curve analysis was to be sufficient to distinguish between the melting of perfect matched dsDNA and dsDNA with the smallest possible change in sequence, a one base pair mismatch.
    Type: Grant
    Filed: December 12, 2016
    Date of Patent: February 19, 2019
    Inventor: Michael D. Okura
  • Patent number: 10188340
    Abstract: A handheld, conforming capacitive sensing apparatus configured to measure Sub-Epidermal Moisture (SEM) as a mean to detect and monitor the formation of pressure ulcers. The device incorporates an array of electrodes which are excited to measure and scan SEM in a programmable and multiplexed manner by a battery-less RF-powered chip. The scanning operation is initiated by an interrogator which excites a coil embedded in the apparatus and provides the needed energy burst to support the scanning/reading operation. Each electrode measures the equivalent sub-epidermal capacitance corresponding and representing the moisture content.
    Type: Grant
    Filed: April 9, 2016
    Date of Patent: January 29, 2019
    Assignees: Bruin Biometrics, LLC, The Regents of the University of California
    Inventors: Majid Sarrafzadeh, William Kaiser, Alireza Mehrnia, Barbara M. Bates-Jensen, Frank Wang, Michael Flesch, Joseph Boystak, Yeung Lam
  • Patent number: 10184934
    Abstract: Provided is a composition comprising an analyte bound covalently or through a first binding pair to a polymer. In this composition, the analyte is less than about 2000 MW; the polymer further comprises more than one signal or first member of a second binding pair; and the analyte is not a member of the first binding pair or the second binding pair. Also provided is an assay for an analyte. The assay comprises: combining a sample suspected of containing the analyte with the above-described composition and a binding agent that binds to the analyte; and detecting the signal or the first member of the second binding pair that is bound to the binding agent. Additionally provided is a multisignal labeling reagent comprising a first polymer covalently bound to (a) a reactive group or a first member of a first binding pair, and (b) more than one digoxigenin molecule.
    Type: Grant
    Filed: May 31, 2017
    Date of Patent: January 22, 2019
    Assignee: Enzo Life Sciences, Inc.
    Inventors: Jack Coleman, Maciej Szczepanik, Richard Jin
  • Patent number: 10180567
    Abstract: An optical modulator element includes a plurality of hole structure, a first liquid reservoir that stores a liquid to be propelled into the plurality of hole structure, a plurality of second liquid reservoirs that store the liquid to be propelled into the plurality of hole structure, and a movement control part that is arranged at at least one end of the second liquid reservoir that is closest to the first liquid reservoir and controls a movement of the liquid from the first liquid reservoir.
    Type: Grant
    Filed: March 10, 2015
    Date of Patent: January 15, 2019
    Assignee: RICOH COMPANY, LTD.
    Inventor: Suguru Sangu
  • Patent number: 10174305
    Abstract: Microfluidic devices and methods for the encapsulation of particles within liquid droplets are disclosed. The new methods and devices form 1-100 picoliter-size monodisperse droplets containing the particles, such as single cells, encapsulated in individual liquid droplets. The particles can be encapsulated in droplets of a fluid by passing a fluid containing the particles through a high aspect-ratio microchannel to order the particles in the fluid, followed by forming the fluid into droplets. The resulting fraction of the liquid droplets with a single particle (e.g., a cell) is higher than the corresponding fraction of single-particle liquid droplets predicted by Poisson statistics.
    Type: Grant
    Filed: June 12, 2017
    Date of Patent: January 8, 2019
    Assignee: The General Hospital Corporation
    Inventors: Jon F. Edd, Mehmet Toner, Dino DiCarlo, Daniel Irimia
  • Patent number: 10156555
    Abstract: The invention relates to a device and a method for determining at least one physical, chemical and/or biological characteristic of a liquid medium that has been introduced into a sealable container, said device comprising at least: one measuring head system comprising at least one pressure measuring unit for measuring the pressure of a gaseous medium and/or of the liquid medium present in the container and a temperature measuring unit for measuring the temperature of the gaseous medium and/or of the liquid medium; one movement system, at least for moving the container; and one determination system, at least for determining the physical, chemical and/or biological characteristic of the liquid medium from at least one of the individual values relating to the measured pressure and the measured temperature of the medium.
    Type: Grant
    Filed: September 9, 2014
    Date of Patent: December 18, 2018
    Assignee: Steinfurth Mess-Systeme GmbH
    Inventor: Martin Falkenstein
  • Patent number: 10147976
    Abstract: A battery assembly heat insulating container includes a box body and a lid body. The box body has an opening on its upper surface, and contains a battery module formed by connecting a plurality of battery cells in series or in parallel. The lid body is placed on the upper surface of the box body. The battery assembly heat insulating container is adapted to provide heat insulation between an external space and an internal space formed by the box body and the lid body and configured to contain the battery module. A circuit member configured to detect leakage of active material from the battery module is provided at the bottom of the box body.
    Type: Grant
    Filed: December 9, 2014
    Date of Patent: December 4, 2018
    Assignee: NGK Insulators, Ltd.
    Inventor: Motohiro Fukuhara
  • Patent number: 10139407
    Abstract: A method for characterizing bacteria includes passing a liquid containing an analyte comprising a first bacteria and a second bacteria over and in contact with a polymer material on a substrate. The polymer material is formulated to bind to the first bacteria, and the first bacteria binds to the polymer material with a higher affinity than the second bacteria. A heat transfer property of the polymer material varies based on an amount of the analyte bound thereto. The method further includes binding a portion of the first bacteria and the second bacteria of the analyte to the polymer material, removing at least a portion of the second bacteria from the polymer material, detecting a temperature of the substrate, and calculating a concentration of the first bacteria in the liquid based at least in part on the temperature of the substrate.
    Type: Grant
    Filed: April 11, 2016
    Date of Patent: November 27, 2018
    Assignees: Universiteit Maastricht, Academisch Ziekenhuis Maastricht
    Inventors: Bart Robert Nicolaas Van Grinsven, Thomas Jan Cleij
  • Patent number: 10132791
    Abstract: A device and system for automatic handling of a sensor strip by a part of a meter that includes a sensor strip having a first section, a second section, and an intermediate section. The sensor strip includes at least a first opening about a first end thereof and a second opening about a second end thereof. A meter part includes a pair of pivoting catches configured to engage and grasp a sensor strip from a container containing a plurality of sensor strips. The sensor strip may thus be removed from a container for testing without need for manual handling of the strip by a user.
    Type: Grant
    Filed: June 16, 2016
    Date of Patent: November 20, 2018
    Assignee: Ascensia Diagnostics Care Holdings AG
    Inventor: Steven C. Charlton
  • Patent number: 10131934
    Abstract: A method for carrying out nucleic acid amplification reactions using a microfluidic device is described. Amplification primers and other amplification reagents are deposited at a plurality of reaction sites in the device, a sample solution containing amplifiable polynucleotides is introduced into the reaction sites, and amplification is carried out.
    Type: Grant
    Filed: November 7, 2017
    Date of Patent: November 20, 2018
    Assignee: Fluidigm Corporation
    Inventors: Marc Unger, Ian D. Manger, Michael Lucero, Yong Yi, Emily Miyashita-Lin, Anja Wienecke, Geoffrey Facer
  • Patent number: 10126296
    Abstract: The present invention provides apparatus and methods for the rapid determination of analytes in liquid samples by immunoassays incorporating magnetic capture of beads on a sensor capable of being used in the point-of-care diagnostic field.
    Type: Grant
    Filed: August 5, 2011
    Date of Patent: November 13, 2018
    Assignee: Abbott Point of Care Inc.
    Inventor: Cary James Miller
  • Patent number: 10119643
    Abstract: A fuel piping structure includes a fuel pipe, a pressure sensor in communication with the fuel pipe through a communication channel, and a sensor holding part including a projecting part projecting to an outer-peripheral side, being connected to the fuel pipe, and holding the pressure sensor disposed therein. A ring-shaped elastic member is provided between an underside of the projecting part and the fuel pipe, the projecting part being pressed against the fuel pipe. The tip side of the sensor holding part on which a ring-shaped seal member is provided is inserted into a recessed part of the fuel pipe and in communication with the inside of the fuel pipe, and the communication channel is in communication with the recessed part. In the sensor holding part, a conduction channel for a seal test is formed from an end face of the projecting part to the seal member.
    Type: Grant
    Filed: September 7, 2016
    Date of Patent: November 6, 2018
    Inventors: Tasuku Yanagimoto, Masaharu Ichise
  • Patent number: 10078054
    Abstract: A system and method is provided for detection at least one of nitrite (NO2), nitric oxide (NO), nitrogen dioxide (NO2), and other nitrogen oxides (NOx) on a substrate, such as skin. According to one aspect, a diagnostic device for measuring a concentration of at least one of nitrite (NO2), nitrogen dioxide (NO2), nitric oxide (NO), and other nitrogen oxides (NOx) on a substrate comprises a patch comprising at least one chemical compound capable of reacting with the at least one of nitrite, nitrogen dioxide, nitric oxide, and other nitrogen oxides to provide an indicator.
    Type: Grant
    Filed: May 7, 2015
    Date of Patent: September 18, 2018
    Assignee: AOBIOME LLC
    Inventors: James Heywood, David R. Whitlock
  • Patent number: 10078078
    Abstract: The invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.
    Type: Grant
    Filed: July 14, 2016
    Date of Patent: September 18, 2018
    Inventors: Vamsee K. Pamula, Michael G. Pollack, Ramakrishna Sista, Arjun Sudarsan
  • Patent number: 10077462
    Abstract: A method and microfluidic device useful for isolating microbes from a blood sample which includes introducing the blood sample into the sample inlet of a spiral microfluidic device; and introducing a second fluid into the sheath inlet of the microfluidic device, wherein the spiral channel terminates in a microbe outlet and a waste outlet, and wherein the spiral channel includes a length, height, and a width that define an aspect ratio adapted to isolate any microbes present in the sample along a first portion of the spiral channel terminating at the microbe outlet, and to isolate red blood cells and leukocytes along a second portion of the spiral channel terminating at the waste outlet; and collecting the microbes from the microbe outlet, thereby isolating the microbes.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: September 18, 2018
    Assignees: The Broad Institute, Inc., Massachusetts Institute of Technology, The General Hospital Corporation
    Inventors: Han Wei Hou, Jongyoon Han, Roby Bhattacharyya, Deb Hung
  • Patent number: 10058864
    Abstract: A microfluidic structure in which a plurality of chambers arranged at different positions are connected in parallel and into which a fixed amount of fluid may be efficiently distributed without using a separate driving source, and a microfluidic device having the same. The microfluidic device includes a platform having a center of rotation and including at least one microfluidic structure. The microfluidic structure includes a sample supply chamber configured to accommodate a sample, a plurality of first chambers arranged in a circumferential direction of the platform at different distances from the center of rotation of the platform, and a plurality of siphon channels, each of the siphon channels being connected to a corresponding one of the first chambers.
    Type: Grant
    Filed: July 20, 2015
    Date of Patent: August 28, 2018
    Inventor: Beom Seok Lee
  • Patent number: 10058861
    Abstract: A technique relates to a fluidic cell configured to hold a nanofluidic chip. A first plate is configured to hold the nanofluidic chip. A second plate is configured to fit on top of the first plate, such that the nanofluidic chip is held in place. The second plate has at least one first port and at least one second port. The second plate has an entrance hole configured to communicate with an inlet hole of the nanofluidic chip. The second port is angled above the first port, such that the first port and second port intersect to form a junction. The second port is formed to have a line-of-sight to the entrance hole, such that the second port is configured to receive input for extracting air trapped at a vicinity of the entrance hole.
    Type: Grant
    Filed: November 25, 2015
    Date of Patent: August 28, 2018
    Inventors: Michael A. Pereira, Joshua T. Smith, Benjamin H. Wunsch