Analyzer, Structured Indicator, Or Manipulative Laboratory Device Patents (Class 422/50)
  • Patent number: 11141729
    Abstract: An object focuser may include a substrate, a sample fluid passage supported by the substrate, a first inertial pump supported by the substrate to pump a sample fluid entraining an object through the sample fluid passage, a first sheath fluid passage, a second inertial pump supported by the substrate to pump a first sheath fluid through the first sheath fluid passage, a second sheath fluid passage and a second inertial pump supported by the substrate to pump a second sheath fluid through the second sheath fluid passage. The first sheath fluid passage and the second sheath fluid passage are connected to the sample fluid passage at a convergence on opposite sides of the sample fluid passage.
    Type: Grant
    Filed: January 24, 2018
    Date of Patent: October 12, 2021
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Viktor Shkolnikov, Alexander N. Govyadinov, Pavel Kornilovich
  • Patent number: 11133516
    Abstract: The present teachings provide multi-reformable fuel delivery systems and methods that can deliver, without the use of a liquid pump, any hydrocarbon fuel, i.e., a liquid or gaseous reformable fuel, for example, to at least one of a reformer, a vaporizer, a fuel cell stack, an afterburner and other assemblies and components of a fuel cell unit or system, More specifically, gas pressure can be used to control and deliver gaseous reformable fuels and/or liquid reformable fuels in the delivery systems and methods of the present teachings. The delivery systems and methods also can apply to the delivery of a liquid reactant such as water and gaseous reactants such as an oxygen-containing gas (e.g., air) and steam.
    Type: Grant
    Filed: August 14, 2015
    Date of Patent: September 28, 2021
    Assignee: WATT Fuel Cell Corp.
    Inventors: Paul DeWald, Caine Finnerty
  • Patent number: 11112233
    Abstract: Aspects of the subject technology relate to an apparatus for self-mixing particulate-matter sensing using a vertical-cavity surface-emitting laser (VCSEL) with extrinsic photodiodes. The apparatus includes a dual-emitting light source disposed on a first chip and to generate a first light beam and a second light beam. The first light beam illuminates a particulate matter (PM), and a light detector extrinsic to the first chip measures the second light beam and variations of the second light beam and generates a self-mixing signal. The variations of the second light beam are caused by a back-scattered light resulting from back-scattering of the first light beam from the PM. The light detector is coupled to the dual-emitting light source. The direction of the second light beam is opposite to the direction of the first light beam, and the second light beam is directed to a sensitive area of the light detector.
    Type: Grant
    Filed: September 12, 2019
    Date of Patent: September 7, 2021
    Assignee: Apple Inc.
    Inventors: Mehmet Mutlu, Michael K. Brown, Wesley S. Smith, Orit A. Shamir, Richard T. Chen, Mark T. Winkler, Miaolei Yan, Richard Yeh
  • Patent number: 11103870
    Abstract: Methods are described herein for isolating clonal populations of cells having a defined genetic modification. The methods are performed, at least in part, in a microfluidic device comprising one or more sequestration pens. The methods include the steps of: maintaining individual cells (or precursors thereof) that have undergone a genomic editing process in corresponding sequestration pens of a microfluidic device; expanding the individual cells into respective clonal populations of cells; and detecting, in one or more cells of each clonal population, the presence of a first nucleic acid sequence that is indicative of the presence of an on-target genome edit in the clonal population of cells. Also described are methods of performing genome editing within a microfluidic device, and compositions comprising one or more clonal populations of cells generated according to the methods disclosed herein.
    Type: Grant
    Filed: January 28, 2019
    Date of Patent: August 31, 2021
    Assignee: Berkeley Lights, Inc.
    Inventors: Gregory G. Lavieu, Annamaria Mocciaro, Xiao Guan Radstrom, Jason M. McEwen, Magali Soumillon, J. Tanner Nevill, Volker L. S. Kurz, Patricia A. Dyck, Ravi K. Ramenani
  • Patent number: 11073530
    Abstract: A sample distribution system 1 has a regulator 4 for receiving a pipetting unit 2 that has at least one exchangeable pipette tip 3, wherein the regulator 4 is configured to change the position of the pipetting unit 2 in relation to a base plate 5, and to detect the presence or absence of pipette tips 3 with a sensor unit 12. According to the invention, the sensor unit 12 is a photoelectric sensor, in particular a reflection photoelectric sensor, with a detection range. The sensor unit 12 and the pipetting unit 2 can also move in relation to one another, in order to determine the presence of a pipette tip 3, if the pipette tip is in the detection range of the sensor unit 12.
    Type: Grant
    Filed: December 19, 2018
    Date of Patent: July 27, 2021
    Assignee: Integra Biosciences AG
    Inventors: Garry Müller, Andreas Städler
  • Patent number: 11002733
    Abstract: The invention relates to a lateral flow device (10) for taking at least one analyte of interest in a fluid, comprising: a fluid receiving zone (100) for receiving the fluid, comprising a first absorbent material (111) that is capable of collecting the fluid; at least one zone of migration (201) for migration of the fluid, extending between a first end and a second end, each zone of migration including a draining material (211) in fluid continuity with the first absorbent material; at least one fluid reservoir (storage) zone (301) comprising a second absorbent material (311) in contact with the draining material at the level of the second end of each fluid migration zone; the detection means between the receiving zone and the reservoir zone, that provide the ability to determine the presence or absence of the said one or more analyte(s) of interest in the sample; characterised in that at least the one of the said zones of migration further also comprises at least one capture zone (400) that is capable of re
    Type: Grant
    Filed: June 24, 2016
    Date of Patent: May 11, 2021
    Assignees: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES, SOCIETE DES SYSTEMES BIOLOGIQUES
    Inventors: Adrien Mombrun, François Berger, Mohamed-Ali Bouamrani, Gary Brandam, Clarence Deffaud, Julien Roux
  • Patent number: 10955380
    Abstract: A nanoscale protein-sensing platform with a non-equilibrium on-off switch that employs dielectrophoretic and hydrodynamic shear forces to overcome these thermodynamic limitations with irreversible kinetics. The detection sensitivity is achieved with complete association of the antibody-antigen-antibody (Ab-Ag-Ab) complex by precisely and rapidly assembling carbon nanotubes (CNT) across two parallel electrodes via sequential DC electrophoresis and dielectrophoresis (DEP), and with single-CNT electron tunneling conductance. The high selectivity is achieved with a critical hydrodynamic shear rate between the activated dissociation shear rates of target and non-target linkers of the aligned CNTs.
    Type: Grant
    Filed: November 14, 2017
    Date of Patent: March 23, 2021
    Assignee: University of Notre Dame du Lac
    Inventors: Hsueh-Chia Chang, Diya Li, Satyajyoti Senapati
  • Patent number: 10935538
    Abstract: A method for assessing the condition of an oil using a portable computer coupled to a camera comprises: separating wear particles from a sample of the oil; capturing an image of the separated wear particles using the camera; and analysing the image and generating information about the condition of the oil using the portable computer. The method may be used for tracking the condition of an oil in a machine over a period of time. The methods are particularly useful for assessing the condition of an industrial oil e.g. a lubricating oil that has been used to lubricate moving parts in a machine.
    Type: Grant
    Filed: May 16, 2017
    Date of Patent: March 2, 2021
    Assignee: Castrol Limited
    Inventors: Sander Gaemers, Bryan Rabenau, Marcel Jerusalem
  • Patent number: 10929641
    Abstract: An automated microscope system is described that detects dicentric chromosomes (DCs) in metaphase cells arising from exposure to ionizing radiation. The radiation dose depends on the accuracy of DC detection. Accuracy is increased using image segmentation methods are used to rank high quality cytogenetic images and eliminate suboptimal metaphase cell data in a sample based on novel quality measures. When a sufficient number of high quality images are detected, the microscope system is directed to terminate metaphase image collection for a sample. The microscope system integrates image selection procedures that control an automated digitally controlled microscope with the analysis of acquired metaphase cell images to accurately determine radiation dose. Early termination of image acquisition reduces sample processing time without compromising accuracy. This approach constitutes a reliable and scalable solution that will be essential for analysis of large numbers of potentially exposed individuals.
    Type: Grant
    Filed: August 7, 2018
    Date of Patent: February 23, 2021
    Assignee: CytoGnomix Inc.
    Inventors: Peter Keith Rogan, Yanxin Li, Jin Liu
  • Patent number: 10928409
    Abstract: A sample receiving assembly capable of receiving a fluid sample from sample containers having different sizes and shapes includes an arm, an arm holder, a sample probe, and a support member, the support member having a bore therethrough and a plurality of linear grooves. The arm is disposed within the arm holder and the sample probe is disposed within the arm. The arm holder includes a hollow pivot pin insertable through the bore in the support member. The sample probe has a first portion extendable through a distal end of the arm, and a second portion extending axially through the hollow pivot pin. The plurality of linear grooves in the support member are sized and positioned to receive a guide pin on the arm and guide retraction of the arm into the arm holder from an extended position to a retracted position.
    Type: Grant
    Filed: December 12, 2017
    Date of Patent: February 23, 2021
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventor: Kenneth P. Galano
  • Patent number: 10913987
    Abstract: The system described herein for bacterial identification can be used as a point-of-care or lab-based diagnostic system. In some implementations, the system can be used to detect other foreign agents within blood or other samples. The system can include disposable microfluidic cartridges that are removable from the system. The microfluidic cartridges can receive a sample, such as a blood sample, that is suspected of containing bacterial cells and separate the bacterial cells from the blood sample. Once the bacterial cells are separated from the blood, the system can introduce the recombinant detector bacteriophages into the system that can infect the bacterial cells. The system can then detect the expression of reporter genes from the recombinant detector bacteriophages.
    Type: Grant
    Filed: March 27, 2017
    Date of Patent: February 9, 2021
    Assignee: The Charles Stark Draper Laboratory, Inc.
    Inventors: Jason W. Holder, Jason O. Fiering
  • Patent number: 10890720
    Abstract: A method of measuring a time-varying signal emission, the method including subjecting the contents of a receptacle to a thermal cycling process. During the thermal cycling process, measuring a signal emission from the contents of the receptacle at regular time intervals and recording the measured signal emission and a time stamp at each time interval. Also during the thermal cycling process, determining a temperature of the thermal cycling process at regular time intervals and recording the determined temperature and a time stamp at each time interval. The measured signal emissions are synchronized with a specific temperature of the thermal cycling process by comparing the time stamps of the measured emission signals with the time stamps of the determined temperatures.
    Type: Grant
    Filed: November 2, 2018
    Date of Patent: January 12, 2021
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Norbert D. Hagen, David Opalsky
  • Patent number: 10837959
    Abstract: A system and method for detecting harmful substances within a consumable sample comprising: receiving a consumable sample at a first chamber of a test container; transforming the consumable sample into a homogenized sample upon processing of the consumable sample; delivering the homogenized sample to a second chamber of the test container, wherein the second chamber is configured to receive the homogenized sample comprises an outlet port; mixing the homogenized sample with a process reagent within the second chamber, thereby producing a dispersion; transmitting a volume of the dispersion to an analysis chamber, of the test container, configured to position a detection substrate proximal the port of the second chamber and comprising a detection window that enables detection of presence of the allergen; and detecting presence of the allergen within the consumable sample by way of an optical sensor configured to detect signals indicative of the allergen through the detection window.
    Type: Grant
    Filed: March 2, 2018
    Date of Patent: November 17, 2020
    Assignee: Nima Acquisition, LLC
    Inventors: Scott Sundvor, Steven Portela, Jonathan Ward, John Walton, Jonathan William Kiel, Jeffrey Mekler, Shireen Yates, Jacob Mooney
  • Patent number: 10809254
    Abstract: Provided herein are methods of splitting droplets containing magnetically responsive beads in a droplet actuator. A droplet actuator having a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at the droplet operations electrodes, is provided. The magnetically responsive beads in the droplet are immobilized using the magnetic field and the plurality of droplet operations electrodes are used to split the droplet into first and second droplets while the magnetically responsive beads remain substantially immobilized.
    Type: Grant
    Filed: November 14, 2018
    Date of Patent: October 20, 2020
    Assignee: ADVANCED LIQUID LOGIC, INC.
    Inventors: Vamsee K. Pamula, Ramakrishna Sista, Vijay Srinivasan, Michael G. Pollack, Allen E. Eckhardt
  • Patent number: 10775284
    Abstract: There is provided a method of charging an array of micro-capillaries. The micro-capillaries have at least one end that is open for fluid communication. The method includes the steps of: (a) filling the array of micro-capillaries with an assay liquid; (b) controllably evaporating at least some of the assay liquid to remove it from the micro-capillary and create a void space in each of the capillaries between the assay liquid and the open end; and (c) filling the void space with a liquid that is immiscible with said assay liquid. There is also provided a use of the disclosed method and a device for charging an array of micro-capillaries.
    Type: Grant
    Filed: May 26, 2016
    Date of Patent: September 15, 2020
    Assignee: JN Medsys Pte Ltd
    Inventors: Kian Kok Johnson Ng, Koon Kiat Teu, Mei Tze Belinda Ling, Jia Wen Sim
  • Patent number: 10717881
    Abstract: An electrical device capable of water-resisting, vapor resistant and electrically conducting in a moisture-containing environment and a method for making such electrical device are provided. The present invention for use in electrically conductive aqueous solutions enables the electrical device to be electrically conductive in any direction because of quantum tunneling effect. The electrical device includes a device body having at least one surface, and a plurality of nano-particles embedded in one of surface pores of the at least one surface of the device body. The particle size of the nano-particles is less than tens of nanometers.
    Type: Grant
    Filed: November 30, 2016
    Date of Patent: July 21, 2020
    Assignee: Dazzeon Technology Co., LTD
    Inventor: Thomas Chen
  • Patent number: 10688491
    Abstract: According to the invention, generally, a method for making a microfluidic aliquoting (MA) chip, adapted to fit in a Petri dish, has a center well (inlet) connected by branched channels to a plurality of side wells (outlets). The chip comes in various types, including a bMA Chip T1, bMA Chip T2, bMA Chip T3, and an rMA Chip. The branched channel improvement provides for a greater distance between neighboring channels and a decreased density near the center well. Design improvements including an injection mold design for an insert and a base and a multiplex hole punch allow for rapid fabrication of the MA chip.
    Type: Grant
    Filed: November 24, 2017
    Date of Patent: June 23, 2020
    Inventor: Lidong Qin
  • Patent number: 10654040
    Abstract: Embodiments are directed to a platform for liquid droplet generation and isolation for biochemical sensing and testing. An embodiment includes generator, fluid-exchange, and manipulator structures that are vertically aligned on a substrate to form a collection chamber. The generator structure is configured to form liquid droplets from a stream of liquid using gas. The fluid-exchange structure is connected to the generator structure to receive the liquid droplets in a carrier liquid held in the collection chamber. The manipulator structure is connected to receive the liquid droplets in the carrier liquid via an inlet. The manipulator structure defines a manipulator chamber connected to the inlet and has a first outlet and a second outlet and a filter capable of filtering the liquid droplets from the carrier liquid. The first outlet enables removal of the liquid droplets filtered and the second outlet enables removal of the carrier liquid.
    Type: Grant
    Filed: August 18, 2017
    Date of Patent: May 19, 2020
    Assignee: Northeastern University
    Inventors: Pooyan Tirandazi Khalilabad, Carlos H. Hidrovo Chaves
  • Patent number: 10646872
    Abstract: A support for conserving samples of biological material comprising at least: a first portion of an absorbent material aimed at and destined to conserve a sample of biological material and a second portion, distinct from the first portion and aimed at, predisposed and destined to constitute a cleaning zone for a head of a device, in particular a punch, aimed at collecting a sample of biological material from the first portion. The support can further comprise a third portion of connection interposed between the first portion and the second portion; the third portion can exhibit one or more cuts or one or more openings.
    Type: Grant
    Filed: June 23, 2014
    Date of Patent: May 12, 2020
    Assignee: Copan Italia S.p.A.
    Inventor: Daniele Triva
  • Patent number: 10646899
    Abstract: According to one embodiment, a cell sorter includes a flow channel which supplies a sample liquid containing particles, a plurality of branch channels connected to the flow channel, an image sensor which has a pixel region covering the flow channel and the branch channels, a determination unit which determines the characteristics of the particles in the sample liquid from a measurement signal of the pixel region, and a separation unit guides the particles in the sample liquid to any of the branch channels based on the determination result of the determination unit.
    Type: Grant
    Filed: March 8, 2018
    Date of Patent: May 12, 2020
    Assignee: KABUSHIKI KAISHA TOSHIBA
    Inventors: Kaita Imai, Shouhei Kousai, Yosuke Akimoto, Michihiko Nishigaki, Yutaka Onozuka, Miyu Nagai
  • Patent number: 10646864
    Abstract: The purpose of the present invention is to provide a detection method which can safely recover a remaining sample from a detection device without causing a biological disaster. In order to achieve at least one of these goals, this detection method involves: a sample collection step for collecting a sample from the sample holding well, and transferring the same to a detection chip; a detection step for detecting the detected substance captured in the detection chip by means of a detection unit; and, after the sample collection step, a remaining sample recovery step for collecting the sample remaining in the sample holding well and transferring this to a sample recovery well. At that time, after the remaining sample recovery step, the surface area of the opening in the sample recovery well is smaller than the surface area of the opening in the sample holding well.
    Type: Grant
    Filed: January 29, 2016
    Date of Patent: May 12, 2020
    Assignee: Konica Minolta, Inc.
    Inventors: Youichi Aoki, Masataka Matsuo, Tetsuya Noda
  • Patent number: 10627390
    Abstract: A method for analyzing a biologic fluid sample includes the steps of: a) providing a spatially mapped chamber; b) providing a predetermined repeatable non-uniform spatial distribution of one or more constituents within the sample, which distribution indicates the presence or absence of a statistically significant number of constituents within the sample in each chamber sub-region; c) selecting one or more image techniques for each sub-region based on the presence or absence of the statistically significant number of one or more constituents in that sub-region as indicated by the distribution; d) creating image data representative of the biologic fluid sample in each sub-region, using the one or more image techniques selected for that sub-region; and e) analyzing the sample.
    Type: Grant
    Filed: August 13, 2018
    Date of Patent: April 21, 2020
    Assignee: Abbott Point of Care, Inc.
    Inventors: Min Xie, Stephen C. Wardlaw
  • Patent number: 10549480
    Abstract: The invention is about an ultrasonic welding-based microfluidic device. It is mainly made of a first element and a second element welded one to the other via at least one structure (10, 10?). The structure (10, 10?) comprises an elongated welded portion for said welding, a welding channel (12, 12?) extending between the first and second elements and along one side of the welded portion, and a draining channel (13) communicating with the welding channel (12, 12?) and the microfluidic path (20, 20?) of the device. The invention is further about a method of manufacturing such a device.
    Type: Grant
    Filed: November 17, 2017
    Date of Patent: February 4, 2020
    Assignee: KONINKLIJKE PHILIPS N.V.
    Inventors: Niels Agersnap Larsen, Klaus Mogensen
  • Patent number: 10544039
    Abstract: Methods for depositing a measured amount of a species in a sealed cavity. In one example, a method for depositing molecules in a sealed cavity includes depositing a selected number of microcapsules in a cavity. Each of the microcapsules contains a predetermined amount of a first fluid. The cavity is sealed after the microcapsules are deposited. After the cavity is sealed the microcapsules are ruptured to release molecules of the first fluid into the cavity.
    Type: Grant
    Filed: September 8, 2017
    Date of Patent: January 28, 2020
    Assignee: TEXAS INSTRUMENTS INCORPORATED
    Inventors: Benjamin Stassen Cook, Kurt Wachtler, Adam Joseph Fruehling, Juan Alejandro Herbsommer, Simon Joshua Jacobs
  • Patent number: 10539560
    Abstract: A substrate for sample analysis is a substrate for sample analysis used for causing a binding reaction between an analyte and a ligand in a liquid sample, the substrate for sample analysis including: a base substrate having a rotation axis and a predetermined thickness; a chamber located in the base substrate for holding a liquid sample containing an analyte and a ligand immobilized on a surface of magnetic particles; and at least one magnet arranged at a position such that the magnetic particles are captured in the chamber by the magnet.
    Type: Grant
    Filed: June 29, 2015
    Date of Patent: January 21, 2020
    Assignee: PHC HOLDINGS CORPORATION
    Inventors: Masayuki Saiki, Masahiro Johno, Fusatoshi Okamoto, Kazuya Kondoh
  • Patent number: 10525461
    Abstract: The present disclosure provides a cartridge for purifying biological samples. The cartridge comprises a first chamber configured to receive and hold biological samples, and at least one second chamber configured to receive and hold a reagent solution. A first fluid flow control valve is fluidly connected to outlet ports of the first chamber and the at least one second chamber. Also, the first fluid flow control valve is fluidly connected to a matrix chamber and is configured to selectively route biological samples and reagent solution into the matrix chamber for purifying the biological samples. A binding matrix is configured in the matrix chamber for capturing the nucleic acids from the biological samples and the reagent solution. Further, a elute collection chamber is fluidly connected to the matrix chamber, wherein the elute collection chamber is configured to receive and hold purified biological sample from the matrix chamber.
    Type: Grant
    Filed: August 30, 2016
    Date of Patent: January 7, 2020
    Assignee: Bigtec Private Limited
    Inventors: Manoj Mulakkapurath Narayanan, Chandrasekhar Bhaskaran Nair
  • Patent number: 10520472
    Abstract: A novel SH-SAW biosensor capable of non-invasive and touch-free detection of cancer cell viability and growth or proliferation in two-dimensional (2D) and three-dimensional (3D) cell cultures as well as stem cell regeneration as it pertains to cancer cell biology and anti-cancer drug development is presented. The biosensor includes two pairs of resonators including interdigital transducers reflecting fingers to quantify mass loading by the cells in suspension as well as within a tumoroid culture platform. The biosensor can be part of a perfused 3PNS-tumoroid system that is amenable to real-time non-invasive monitoring of the cell proliferation, viability, and multiplexed detection of key physiologic and clinical biomarkers.
    Type: Grant
    Filed: August 21, 2017
    Date of Patent: December 31, 2019
    Assignees: University of South Florida, TransGenex Nanobiotech, Inc.
    Inventors: Shyam S. Mohapatra, Subhra Mohapatra, Rasim Oytun Guldiken, Rajesh R. Nair, Tao Wang
  • Patent number: 10509007
    Abstract: Interference signals from other gases in an air sample are eliminated by flowing an air sample directly to an electrochemical gas sensor and subsequently flowing an air sample through a selective scrubber and removing the target gas of interest before flowing the sample to the electrochemical gas sensor. The difference in responses of the electrochemical gas sensor is directly proportional to the concentration of the target gas of interest in the air sample.
    Type: Grant
    Filed: December 18, 2015
    Date of Patent: December 17, 2019
    Assignee: Aeroqual Limited
    Inventor: Geoffrey Stephen Henshaw
  • Patent number: 10501795
    Abstract: A probe system comprising (a) a first single-stranded oligonucleotide labelled with detectable elements in an undetectable state, and (b) second and third single-stranded oligonucleotides capable of hybridising to complementary regions on the first oligonucleotide.
    Type: Grant
    Filed: November 9, 2017
    Date of Patent: December 10, 2019
    Assignee: BASE4 INNOVATION LTD
    Inventors: Barnaby Balmforth, Cameron Alexander Frayling
  • Patent number: 10495637
    Abstract: A universal sensor fabrication approach, molecular substrate imprinting technique, which utilizes the interaction between molecular building blocks and the surface of a transducer to develop specific molecular recognition cavities has been established. Integration of molecular recognition cavities with the surface of a nanoscale transducer will result in a nano-tunneling effect that takes place which will provide a sensor or a device that exhibits new properties not already exhibited by either the molecular recognition cavities on a bulk transducer or the nanotransducer material. One of the new properties of this nano-tunneling effect is that a universal potentiometric molecular sensor can be fabricated and used to detect any compounds, whether they are ions or molecules, with enhanced selectivity, sensitivity, and stability when molecular recognition cavities or elements are integrated on the surface of a nanoscale transducer.
    Type: Grant
    Filed: May 6, 2015
    Date of Patent: December 3, 2019
    Inventor: Yanxiu Zhou
  • Patent number: 10493445
    Abstract: The invention relates to a fluidic system comprising at least one bead chamber (311) containing a lyophilized reagent (LB) and a reaction chamber in a cartridge. In one embodiment, a series of bead chambers with different lyophilized reagents may be provided such that sample fluid can sequentially pass through them. In another embodiment, bead chambers may be located on a movable carrier, for example a rotating carousel, from which they may selectively be connected to a reaction chamber in a cartridge. In still another embodiment, the bead chamber (311) may comprise at least one flexible wall (FW) allowing for a minimization of dead volume associated with the extraction of lyophilized reagent (LB).
    Type: Grant
    Filed: April 29, 2014
    Date of Patent: December 3, 2019
    Assignee: Koninklijke Philips N.V.
    Inventors: Anke Pierik, Reinhold Wimburger-Friedl, Johannes Theodorus Wilhelmus Maria Van Eemeren, Irene Johanna Monia Dobbelaer-Bosboom, Martinus Johannes Van Zelst, Bernadet Dagmar Marielle Meijering
  • Patent number: 10457907
    Abstract: An electrochemical measurement device includes: a base; a specimen placement portion disposed on the base, for placing a biological specimen; a first electrode disposed on the base and surrounding the specimen placement portion; and a first insulating layer covering the first electrode. The first insulating layer has a plurality of openings, and the first electrode has a plurality of first electrode exposed portions which are portions of the first electrode that are exposed from the openings of the first insulating layer.
    Type: Grant
    Filed: September 27, 2016
    Date of Patent: October 29, 2019
    Assignee: PANASONIC INTELLECTUAL PROPERTY MANAGEMENT CO., LTD.
    Inventors: Atsushi Shunori, Masahiro Yasumi, Makoto Takahashi
  • Patent number: 10444184
    Abstract: An assembly is provided for interfacing with a microfluidic chip having at least one microscopic channel configured to receive a liquid sample for analysis. The assembly includes a chip carrier, an electronics module, an optical module, and a mechanical module. The chip carrier includes a base and a cover defining a cavity to receive the microfluidic chip. The electronics module includes a signal generator which applies at least one electrokinetic signal electrode(s) of the chip. The optical module includes an excitation radiation source which causes excitation radiation to impinge on the sample, and an emission radiation detector which detects radiation emitted from the sample. The mechanical module includes a chip-carrier receiving structure, relatable with respect to the optical module for focus and at least one degree of translational freedom.
    Type: Grant
    Filed: June 30, 2016
    Date of Patent: October 15, 2019
    Assignee: International Business Machines Corporation
    Inventors: Jaione T. Azpiroz, Emmanuel Delamarche, Claudius Feger, Ricardo L. Ohta, Mathias B. Steiner, Yuksel Temiz
  • Patent number: 10434206
    Abstract: A closure for a medical sterile container having a trough-like first container part and a lid-type second container part includes a closure flap which can be arranged on one of the container parts so as to be able to pivot between a closed position, in which the container parts are interlocked with each other by the closure, and an open position in which the container parts are unlocked. The closure includes a latch element which in the closed position of the closure interlocks the closure flap with the other container part.
    Type: Grant
    Filed: November 27, 2015
    Date of Patent: October 8, 2019
    Assignee: Aesculap AG
    Inventors: Stefan Thomas, Serkan Bellikli, Matthias Schweizer, Stefan Schuster, Dieter Weisshaupt
  • Patent number: 10422783
    Abstract: Apparatus includes a pivotable, cradling framework that can hold a rotatable bomb instrument, which can include a hollow cylinder and a grease rack. This may be employed to effect a significantly improved version of the ASTM D942 Test Method. The same is useful in measurement of grease oxidation.
    Type: Grant
    Filed: October 17, 2018
    Date of Patent: September 24, 2019
    Assignee: TANNAS COMPANY
    Inventors: Theodore W. Selby, Jonathan C. Evans, Gregory C. Miiller, Calvin David Hugo, Sarah R. Nuss-Warren, Douglas J. Wirsing
  • Patent number: 10379102
    Abstract: A single molecule sensing or detecting device includes a first electrode and a second electrode separated from the first electrode by a gap. The first electrode and the second electrode have an opening formed therethrough. At least one of the first electrode and the second electrode is functionalized with a recognition molecule. The recognition molecule has an effective length L1 and is configured to selectively bind to a target molecule having an effective length L2. The size of the gap is configured to be greater than L2, but less than or equal to the sum of L1 and L2.
    Type: Grant
    Filed: December 12, 2016
    Date of Patent: August 13, 2019
    Assignee: Arizona Board of Regents on behalf of Arizona State University
    Inventors: Stuart Lindsay, Peiming Zhang, Yanan Zhao
  • Patent number: 10371688
    Abstract: A sensing system for explosives is provided. The sensor is based on a layered structure of approximately a monolayer of a fluorophore deposited onto a few nm of a transparent polymer, supported by a substrate. The fluorophores can be xanthene laser dyes, which have high quantum yields, and the polymers can be commodity materials polymethylmethacrylate and polyvinylidene difluoride. The different fluorophore/polymer combinations give different emission responses to analytes, including both signal quenching and enhancement. The pattern of responses can be used to identify the analyte. The common explosives TNT, PETN, RDX, HMX, and TATP as gas phase species can all be uniquely identified at room temperature using only the natural vapor pressure of the explosive to deliver sample to the sensor.
    Type: Grant
    Filed: February 10, 2017
    Date of Patent: August 6, 2019
    Inventor: William B. Euler
  • Patent number: 10292586
    Abstract: The present invention relates to a non-invasive health indicator monitoring system including a sensing module, an electric power storage module, and a circuit module to collect health indicator information by contacting with a subject. In addition, the present invention also relates to a method for monitoring health indicator continuously by using the health indicator monitoring system.
    Type: Grant
    Filed: November 11, 2014
    Date of Patent: May 21, 2019
    Assignee: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Yong Won Song, Su Youn Lee, Ji Yeon Lee, Jung Ah Lim, Ji Won Choi, Byung Ki Cheong, Jin Seok Kim, Ho Seong Jang, Hyun Jung Yi
  • Patent number: 10279343
    Abstract: A transfer device for the contamination free transfer of biological material from a sealed chamber to a recipient. The transfer device includes a support having a first docking area and a second docking area. The first docking area has at least one inlet port, each inlet port creating a fluid connection with one sealed chamber. The second docking area has at least one outlet port, each outlet port creating a fluid connection with a recipient. The first docking area further includes a piercing element designed for piercing a sealing element of a sealed chamber in order to allow, together with at least one inlet port, a fluid connection between a pierced sealed chamber and the inlet port, to allow a contamination free transfer of the biological material from the pierced sealed chamber to at least one recipient.
    Type: Grant
    Filed: October 24, 2014
    Date of Patent: May 7, 2019
    Assignee: Biocartis NV
    Inventors: Nicolas Vergauwe, Thierry Tollenboom
  • Patent number: 10279350
    Abstract: Methods of generating droplets. In an exemplary method, a device including a sample well, a carrier well, a droplet well, and a plurality of microfluidic channels is selected. The microfluidic channels include a first channel, a second channel, and a third channel. A discrete volume of sample-containing fluid is placed into the sample well, and a discrete volume of carrier fluid is placed into the carrier well. A pressure differential is created after placing the discrete volumes, to cause fluid flow. Sample-containing fluid flows from the sample well to a droplet-generation region of the device via the first channel. Carrier fluid flows from the carrier well to the droplet-generation region via the second channel. Sample-containing droplets and carrier fluid flow from the droplet-generation region to the droplet well via the third channel.
    Type: Grant
    Filed: November 30, 2016
    Date of Patent: May 7, 2019
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Benjamin J. Hindson, Kevin D. Ness, Billy W. Colston, Jr., Fred P. Milanovich, Donald A. Masquelier
  • Patent number: 10261012
    Abstract: This disclosure concerns methods and apparatus for interferometric spectroscopic measurements of particles with higher signal to noise ratio utilizing an infrared light beam that is split into two beams. At least one beam may be directed through a measurement volume containing a sample including a medium. The two beams may then be recombined and measured by a detector. The phase differential between the two beams may be selected to provide destructive interference when no particle is present in the measurement volume. A sample including medium with a particle is introduced to the measurement volume and the detected change resulting from at least one of resonant mid-infrared absorption, non-resonant mid-infrared absorption, and scattering by the particle may be used to determine a property of the particle. A wide range of properties of particles may be determined, wherein the particles may include living cells.
    Type: Grant
    Filed: June 6, 2016
    Date of Patent: April 16, 2019
    Assignee: 1087 Systems, Inc.
    Inventor: Matthias Wagner
  • Patent number: 10260168
    Abstract: Contemplated microfluidic devices and methods are drawn to protein arrays in which distinct and detergent-containing antigen preparations are deposited onto an optical contrast layer in a non-specific and non-covalent manner. Detection of binding a is carried out using a dye that precipitates or agglomerates to so form a visually detectable signal at a dynamic range of at least three orders of magnitude.
    Type: Grant
    Filed: February 20, 2012
    Date of Patent: April 16, 2019
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Abraham Lee, Philip Felgner, Armando Tovar, Uland Liao
  • Patent number: 10252266
    Abstract: In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.
    Type: Grant
    Filed: July 3, 2018
    Date of Patent: April 9, 2019
    Assignee: COMBINATI INCORPORATED
    Inventors: Ju-Sung Hung, Andrew Zayac, Megan Dueck
  • Patent number: 10227634
    Abstract: The present disclosure relates generally to the integration of isotachophoresis (ITP) and isothermal nucleic acid amplification methods such as recombinase polymerase amplification (RPA). One aspect of the disclosure relates to a method for concentrating and amplifying a nucleic acid, the method including an isotachophoresis device, the isotachophoresis device including a porous matrix, and first and second electrodes, having a leading electrolyte, a trailing electrolyte and a set of isothermal nucleic acid amplification reaction reagents disposed in the porous matrix as described herein, and applying a voltage across the first electrode and the second electrode for a time sufficient to provide a first isotachophoresis (ITP) plug comprising an amplification product of the nucleic acid, wherein the concentration of the nucleic acid is substantially higher in the first FTP plug than in the first and/or second fluids outside of the first ITP plug.
    Type: Grant
    Filed: April 14, 2016
    Date of Patent: March 12, 2019
    Assignee: University of Washington
    Inventors: Jonathan D. Posner, Mark D. Borysiak, Kevin Kimura
  • Patent number: 10229564
    Abstract: An apparatus provides tactile stimulus to a human user using a tri-layer actuator comprising: first and second polymer layers which are electronically and ionically conductive and have one or more dimensions which vary in dependence on insertion of ions therein and withdrawal of ions therefrom; and a deformable layer located between the first and second polymer layers to physically separate the first and second polymer layers from one another, the deformable layer electronically insulating and ionically conductive. Application of first and second voltages between the first and second polymer layers creates corresponding first and second distributions of ions within the first and second polymer layers. Changing between application of the first and second voltages causes corresponding deformation of the tri-layer actuator between corresponding first and second shapes, a difference or transition between the first and second shapes detectable by a sense of touch of a human user.
    Type: Grant
    Filed: August 31, 2017
    Date of Patent: March 12, 2019
    Assignee: The University of British Columbia
    Inventors: Saeedeh Ebrahimi Takalloo, John D. W. Madden, Shahriar Mirabbasi, Meisam Farajollahi
  • Patent number: 10222254
    Abstract: A sample analyzing apparatus for performing an optical-based measurement on a sample includes a housing, a first light source, excitation optics, a first light detector, emission optics, and a monitoring system, all of which are disposed in the housing. The monitoring system is configured for monitoring a movable component disposed in the housing. The monitoring system includes one or more light sources for illuminating the movable component, and one or more light detectors for detecting light reflected from the movable component in response to being illuminated.
    Type: Grant
    Filed: November 7, 2016
    Date of Patent: March 5, 2019
    Assignee: Molecular Devices (Austria) GmbH
    Inventors: Michael Katzlinger, Georg Kronberger, Bernhard Schinwald
  • Patent number: 10208334
    Abstract: Systems, methods and compositions of matter according to the present invention, can be used in capture/enrichment, gene expression profiling and targeted sequencing. Provided are systems, methods and compositions concerning the enhancement of nucleic acid hybridization specificity and controlling the shapes of melting curves revealed by nucleic acid hybrid pairs to optimize nucleic acid analysis. These systems, methods and compositions comprise producing a positively charged surface or surface coating, on the surface of microarray slides or other types of surfaces similarly purposed, which enhances melting curve analysis to the point of allowing detection or differentiation of small changes in sequences between nucleic acid binding partners. The accuracy or resolution of melting curve analysis was to be sufficient to distinguish between the melting of perfect matched dsDNA and dsDNA with the smallest possible change in sequence, a one base pair mismatch.
    Type: Grant
    Filed: December 12, 2016
    Date of Patent: February 19, 2019
    Inventor: Michael D. Okura
  • Patent number: 10188340
    Abstract: A handheld, conforming capacitive sensing apparatus configured to measure Sub-Epidermal Moisture (SEM) as a mean to detect and monitor the formation of pressure ulcers. The device incorporates an array of electrodes which are excited to measure and scan SEM in a programmable and multiplexed manner by a battery-less RF-powered chip. The scanning operation is initiated by an interrogator which excites a coil embedded in the apparatus and provides the needed energy burst to support the scanning/reading operation. Each electrode measures the equivalent sub-epidermal capacitance corresponding and representing the moisture content.
    Type: Grant
    Filed: April 9, 2016
    Date of Patent: January 29, 2019
    Assignees: Bruin Biometrics, LLC, The Regents of the University of California
    Inventors: Majid Sarrafzadeh, William Kaiser, Alireza Mehrnia, Barbara M. Bates-Jensen, Frank Wang, Michael Flesch, Joseph Boystak, Yeung Lam
  • Patent number: 10184934
    Abstract: Provided is a composition comprising an analyte bound covalently or through a first binding pair to a polymer. In this composition, the analyte is less than about 2000 MW; the polymer further comprises more than one signal or first member of a second binding pair; and the analyte is not a member of the first binding pair or the second binding pair. Also provided is an assay for an analyte. The assay comprises: combining a sample suspected of containing the analyte with the above-described composition and a binding agent that binds to the analyte; and detecting the signal or the first member of the second binding pair that is bound to the binding agent. Additionally provided is a multisignal labeling reagent comprising a first polymer covalently bound to (a) a reactive group or a first member of a first binding pair, and (b) more than one digoxigenin molecule.
    Type: Grant
    Filed: May 31, 2017
    Date of Patent: January 22, 2019
    Assignee: Enzo Life Sciences, Inc.
    Inventors: Jack Coleman, Maciej Szczepanik, Richard Jin
  • Patent number: 10180567
    Abstract: An optical modulator element includes a plurality of hole structure, a first liquid reservoir that stores a liquid to be propelled into the plurality of hole structure, a plurality of second liquid reservoirs that store the liquid to be propelled into the plurality of hole structure, and a movement control part that is arranged at at least one end of the second liquid reservoir that is closest to the first liquid reservoir and controls a movement of the liquid from the first liquid reservoir.
    Type: Grant
    Filed: March 10, 2015
    Date of Patent: January 15, 2019
    Assignee: RICOH COMPANY, LTD.
    Inventor: Suguru Sangu