With Coagulometer For Agglutination, Clotting, Or Prothrombin, Or For Particle (e.g., Cell, Etc.) Counting, Or Volume Or Characteristics Determination Patents (Class 422/73)
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Patent number: 8187888Abstract: The fluidic system of the preferred embodiment includes a sheath pump to pump sheath fluid from a sheath container into an interrogation zone and a waste pump to pump waste fluid from the interrogation zone into a waste container. The sheath pump and/or the waste pump draw sample fluid from a sample container into the interrogation zone. The fluidic system also includes a controller to adjust the flow rate of the sample fluid from the sample container into the interrogation zone. The fluidic system is preferably incorporated into a flow cytometer with a flow cell that includes the interrogation zone.Type: GrantFiled: July 8, 2011Date of Patent: May 29, 2012Assignee: Accuri Cytometers, Inc.Inventor: Collin A. Rich
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Patent number: 8187886Abstract: The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.Type: GrantFiled: January 10, 2011Date of Patent: May 29, 2012Assignee: The General Hospital CorporationInventors: Denise L. Faustman, Douglas E. Burger
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Patent number: 8187538Abstract: An immunodiagnostic test card includes a flat planar member and at least one dilution chamber that is supported by the flat planar member. The at least one dilution chamber can be disposed adjacent chambers used for testing a patient sample that are provided on the immunodiagnostic test card or can be provided separately.Type: GrantFiled: January 17, 2008Date of Patent: May 29, 2012Assignee: Ortho-Clinical Diagnostics, Inc.Inventors: Raymond Francis Jakubowicz, William G. Atterbury, Douglas E. Boyd, Michael L. Marshall
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Patent number: 8178342Abstract: The present invention provides methods and systems for performing in vivo flow cytometry. In one embodiments, selected circulating cells of interest of a subject are labeled with fluorescent probe molecules. The labeled cells are irradiated in vivo so as to excite the fluorescent probes, and the radiation emitted by the excited probes is detected, preferably confocally. The detected radiation is then analyzed to derive desired information, such as relative cell count, of the cells of interest.Type: GrantFiled: January 7, 2009Date of Patent: May 15, 2012Assignee: The General Hospital CorporationInventor: Charles P. Lin
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Publication number: 20120107851Abstract: The present invention is directed to a lateral flow assay device for the monitoring and measuring of coagulation and method thereof. Ideally, the invention is directed to a lateral capillary flow device for the monitoring and/or measurement of coagulation in a liquid sample wherein the device comprises a non-porous substrate with a zone for receiving a sample and a defined flow path zone wherein a clotting agent is deposited on at least part of the defined flow path zone to accelerate the coagulation of the liquid sample, enable the formation of an evenly distributed clot along the defined flow path zone and to result in the change in flow rate or cessation of flow of the liquid sample along the defined flow path zone.Type: ApplicationFiled: April 23, 2010Publication date: May 3, 2012Inventors: Anthony Joseph Killard, Magdalena Maria Dudek, Brian MacCraith, Ib Mendel-Hartvig, Ove Öhman
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Patent number: 8163562Abstract: The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.Type: GrantFiled: August 23, 2010Date of Patent: April 24, 2012Assignee: Life Technologies CorporationInventors: Tom Knapp, Gregory Zlokarnik, Paul Negulescu, Roger Tsien, Timothy Rink
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Patent number: 8158439Abstract: The present invention is to present a sample analyzer which is automated even when measuring a small amount sample and is capable of improving a measurement precision. The sample analyzer S comprises a sample preparation section for preparing a measurement sample; a detector D3 for detecting an analyte contained in the measurement sample; and a controller 100 being configured to 1) control the sample preparation section so as to prepare a first measurement sample of a first dilution ratio, when measuring an ordinary amount sample; and 2) control the sample preparation section so as to prepare a second measurement sample of a second dilution ratio higher than the first dilution ratio, when measuring a small amount sample.Type: GrantFiled: July 16, 2008Date of Patent: April 17, 2012Assignee: Sysmex CorporationInventor: Masaharu Shibata
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Patent number: 8148094Abstract: The invention provides methods and compositions for simultaneously detecting the activation state of a plurality of proteins in single cells using flow cytometry. The invention further provides methods and compositions of screening for bioactive agents capable of coordinately modulating the activity of a plurality of proteins in single cells. The methods and compositions can be used to determine the protein activation profile of a cell for predicting or diagnosing a disease state, and for monitoring treatment of a disease state.Type: GrantFiled: February 17, 2009Date of Patent: April 3, 2012Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Garry P. Nolan, Omar D. Perez
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Patent number: 8137987Abstract: Bodily fluid is analyzed for the presence of drugs of a selected panel of drugs in a simultaneous assay in which sample of the fluid is incubated with additional amounts of all drugs of the panel, antibodies specific to each of the drugs of the panel, and microparticles, the microparticles being divided into subsets, one subset for each drug in the panel and each subset distinguishable from the others. The incubation is performed in a liquid medium in which competitive binding occurs, the drugs in the sample competing with those added to the assay medium for binding to the antibodies. In one procedure, the added drugs are pre-coupled to the microparticles while the antibodies are not, and the incubation is followed by further incubating the microparticles with labeled ligands that have affinity for the antibodies.Type: GrantFiled: April 13, 2011Date of Patent: March 20, 2012Assignee: Bio-Rad Laboratories, Inc.Inventors: William F. Link, Renato B. del Rosario, Randy V. Sweet, David L. King
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Patent number: 8137975Abstract: A method for identifying a platelet population, preferably a population of immature, reticulated platelets, in a biological sample involves incubating the biological sample for less than 5 minutes with at least one labeled, ligand (e.g., monoclonal antibody) that binds to an epitope or antigen on platelets and with a nucleic acid dye. In one embodiment, the dye is Acridine Orange and the label on the ligand is PE-Cy7. The sample is then analyzed and one or more platelet populations is rapidly identified or quantified by passing the incubated sample through a sensing region of a flow cytometer. In one embodiment, this method occurs without a washing or physical cell separation step. The incubated sample is irradiated with a laser light source, and fluorescence of the labeled ligand and the nucleic acid dye are measured along with at least one additional parameter, e.g., light scatter, direct current, axial light loss, opacity, radio frequency, and fluorescence.Type: GrantFiled: October 29, 2008Date of Patent: March 20, 2012Assignee: Beckman Coulter, Inc.Inventors: Ravindra Mylvaganam, Ronald D. Paul
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Patent number: 8133738Abstract: A method for determining the hematocrit of a blood sample is provided that includes the steps of: 1) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by the interior surfaces of first and second panels and a height extending there between, wherein both panels are transparent, and the height is such that at least some of the red blood cells within the sample contact both interior surfaces of the panels and one or more lacunae within the quiescent sample extend between the interior surfaces; 2) imaging at least a portion of the quiescent sample, which sample portion contains the red blood cells and one or more lacunae to determine an optical density of the imaged portion of the sample on a per image unit basis; 3) selecting and averaging the optical density values of the image units aligned with the red blood cells contacting the interior surfaces, and assigning an upper boundary value of 100% to the average optical density value of those imType: GrantFiled: May 26, 2011Date of Patent: March 13, 2012Assignee: Abbott Point of Care, Inc.Inventors: Robert A. Levine, Stephen C. Wardlaw, Darryn Unfricht, Niten V. Lalpuria
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Patent number: 8134122Abstract: Systems, methods and computer program products for the multi-modal detection of particles are described herein. An embodiment of the present invention is a particle detector that includes a first chamber wherein analyte particles are subjected to a first particle detection mechanism, and a second chamber coupled to the first chamber, wherein the analyte particles are subjected to a second particle detection mechanism, and wherein the detection characteristics of second particle detection mechanism are orthogonal to detection characteristics of the first particle detection mechanism.Type: GrantFiled: January 19, 2010Date of Patent: March 13, 2012Assignee: The Mitre CorporationInventor: Samar K. Guharay
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Patent number: 8128890Abstract: The enumeration of cells in fluids by flow cytometry is widely used across many disciplines such as assessment of leukocyte subsets in different bodily fluids or of bacterial contamination in environmental samples, food products and bodily fluids. For many applications the cost, size and complexity of the instruments prevents wider use, for example, CD4 analysis in HIV monitoring in resource-poor countries. The novel device, methods and algorithms disclosed herein largely overcome these limitations. Briefly, all cells in a biological sample are fluorescently labeled, but only the target cells are also magnetically labeled. The labeled sample, in a chamber or cuvet, is placed between two wedge-shaped magnets to selectively move the magnetically labeled cells to the observation surface of the cuvet. An LED illuminates the cells and a CCD camera captures the images of the fluorescent light emitted by the target cells.Type: GrantFiled: November 1, 2010Date of Patent: March 6, 2012Assignee: Veridex, LLCInventors: Erik Droog, Dhanesh Gohel, Arjan G. J. Tibbe, Jan Greve, Leon W. M. M. Terstappen
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Patent number: 8123044Abstract: An improved actuator for use in a microfluidic particle sorting system utilizes a staggered packing scheme for a plurality of actuators used to selectively deflect a particle in an associated sorting channel from a stream of channels. An actuator block may be provided for housing a two-dimensional array of actuators, each configured to align with an actuation port in an associated sorting chip containing a plurality of sorting channels. The actuator block may include a built-in stressing means to pre-stress each actuator housed by the block. An actuator comprising a piezo-electric stack may employ contact-based electrical connection rather than soldered wires to improve packing density. The actuator may be an external actuator. That is, the external actuator is external to the substrate in which the sorting channels are formed.Type: GrantFiled: May 4, 2007Date of Patent: February 28, 2012Assignee: Cytonome/ST, LLCInventors: Andrew Johnson, John R. Gilbert, Manish Deshpande, Hugh Lewis, Bernard Bunner
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Publication number: 20120040446Abstract: An apparatus and a related method for performing particle agglutination reactions in at least one disposable probe tip are disclosed. The at least one probe tip includes a sample cavity for sample acquisition, at least one flanking cavity for the capture of particles by centrifugation or other means, a transition zone for the mixing of the sample with reagents for agglutination and a detection zone for the optical detection of particle agglutination. A mechanism may be attached to the probe tip for the controlled movement of fluids through the internal volume of the probe tip. The probe tip is particularly useful for the automation of high-throughput agglutination-type assays.Type: ApplicationFiled: October 24, 2011Publication date: February 16, 2012Applicant: Ortho-Clinical Diagnostics, Inc.Inventors: Zhong Ding, Amy M. Wilson-Colley
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Patent number: 8114680Abstract: A tube and float system for use in separation and axial expansion of the buffy coat includes a transparent or semi-transparent, flexible sample tube and a rigid separator float having a specific gravity intermediate that of red blood cells and plasma. The float includes a main body portion of reduced diameter to provide a clearance gap between the inner wall of the sample tube and the float. One or more protrusions on the main body portion serve to support the flexible tube. During centrifugation, the centrifugal force causes the diameter of the flexible tube to expand and permit density-based axial movement of the float in the tube. The float further includes a pressure relief system to alleviate pressure build up in the trapped red blood cell blood fraction below the float, thereby preventing red blood cells from being forced into the annular gap containing the buffy coat layers.Type: GrantFiled: March 21, 2011Date of Patent: February 14, 2012Assignee: Battelle Memorial InstituteInventors: Thomas Haubert, Vince Contini, Steve Grimes, Randy Jones, Stephen C. Wardlaw
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Patent number: 8115931Abstract: A photoacoustic detector for measuring a concentration of fine dust particles in gas. The detector includes at least one acoustic sensor for detecting an acoustic signal. Additionally, the detector includes a pulsed light source for providing excitation light having a configurable pulse length and a configurable pulse repetition rate, wherein by changing the pulse length and/or the pulse repetition rate, a size distribution of the fine dust particles can be determined.Type: GrantFiled: March 27, 2008Date of Patent: February 14, 2012Assignee: Fraunhofer-Gesellschaft zur Foerderung der Angewandten Forschung E.V.Inventors: Andras Miklos, Judit Angster
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Publication number: 20120020831Abstract: The airflow controlling device includes a bacteria counting portion counting bacteria of a controlled space; a first smoothing processing portion performing a first smoothing process on the bacteria count; a second smoothing processing portion performing a second smoothing process on the bacteria count; a bacteria reducing capability storing portion storing a bacteria reducing capability relative to each flow rate; a first flow rate evaluating portion selecting a flow rate matching a bacteria reducing capability compatible with an increase in a bacteria count forecasted from the processing result of the first smoothing processing portion; a second flow rate evaluating portion selecting a flow rate matching a bacteria reducing capability compatible with an increase in a bacteria count forecasted from the processing result of the second smoothing processing portion; and a flow rate determining portion selecting a flow rate into the controlled space based on the flow rates selected by the first and second flow rType: ApplicationFiled: July 6, 2011Publication date: January 26, 2012Applicant: Yamatake CorporationInventors: Masato Tanaka, Mayumi Miura, Kenji Akai, Shuji Sawada, Yasuko Horiguchi
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Publication number: 20120020838Abstract: An automatic analysis device 1a includes: an intermediate disk (intermediate unit) 20 on which a disposable container (intermediate container) 21 for carrying out a pretreatment by pretreatment liquid for a sample dispensed from a sample container 11 or a reaction with a reagent is arranged; a reaction disk (main analysis unit) 60 for carrying out a test of an item having a relatively large number of test requests, on which a reaction vessel 61 for carrying out a reaction of a reagent with the sample obtained after the pretreatment is arranged; and flow-based analysis mechanisms (sub analysis units) 30a to 30c for carrying out a test for the sample obtained after the reaction with the reagent in the disposable container 21, which is the item having a relatively small number of test requests.Type: ApplicationFiled: April 8, 2010Publication date: January 26, 2012Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATIONInventors: Tomonori Mimura, Akihisa Makino, Sakuichiro Adachi
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Patent number: 8092758Abstract: A sample acquiring device for volumetric enumeration of white blood cells in a blood sample comprises a measurement cavity for receiving a blood sample. The measurement cavity has a predetermined fixed thickness. The sample acquiring device further comprises a reagent, which is arranged in a dried form on a surface defining the measurement cavity. The reagent comprises a hemolysing agent for lysing red blood cells in the blood sample, and a staining agent for selectively staining white blood cells in the blood sample. A system comprises the sample acquiring device and a measurement apparatus. The measurement apparatus comprises a sample acquiring device holder, a light source, and an imaging system for acquiring a digital image of a magnification of the sample. The measurement apparatus further comprises an image analyser arranged to analyse the acquired digital image for determining the number of white blood cells in the blood sample.Type: GrantFiled: March 10, 2006Date of Patent: January 10, 2012Assignee: Hemocue ABInventors: Stellan Lindberg, Johnny Svensson
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Publication number: 20110318229Abstract: There is provided a droplet discharging apparatus for cell counting that includes: a droplet flowing unit into which a droplet is injected; a pumping unit allowing the injected droplet to flow or to stop flowing; a droplet discharging unit discharging the droplet to a target unit or a receiving unit; a detection unit installed on a droplet discharging route, and irradiating light to the droplet and receiving light reflected on the droplet to generate a light receiving signal; and a control unit performing a control operation by counting the number of cells by receiving the light receiving signal from the detection unit and determining whether or not the cell is present on the basis of the level of the received light receiving signal. Therefore, it is possible to discharge the droplet by accurately counting the number of cells in the droplet.Type: ApplicationFiled: October 11, 2010Publication date: December 29, 2011Applicant: SAMSUNG ELECTRO-MECHANICS CO., LTD.Inventors: Se Hoon Jeong, Sang Youl Jeon, Bo Sung Ku
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Patent number: 8076144Abstract: A hemostasis analyzer, such as the Thrombelastograph® (TEG®) hemostasis analyzer is utilized to measure continuously in real time, the hemostasis process from the initial fibrin formation, through platelet-fibrin interaction and lysis to generate blood hemostasis parameters. The measured blood hemostasis parameters permit preparation of an individualized assessment of ischemic event risk and individualized treatment of a subject.Type: GrantFiled: January 21, 2011Date of Patent: December 13, 2011Assignee: Cora Healthcare, Inc.Inventor: Eli Cohen
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Patent number: 8076095Abstract: A method for the enumeration of micronucleated erythrocyte populations while distinguishing platelet and platelet-associated aggregates involves the use of a first fluorescent labeled antibody having binding specificity for a surface marker for reticulocytes, a second fluorescent labeled antibody having binding specificity for a surface marker for platelets, and a nucleic acid staining dye that stains DNA (micronuclei) in erythrocyte populations. Because the fluorescent emission spectra of the first and second fluorescent labeled antibodies do not substantially overlap with one another or with the emission spectra of the nucleic acid staining dye, upon excitation of the labels and dye it is possible to detect the fluorescent emission and light scatter produced by the erythrocyte populations and platelets, and count the number of cells from one or more erythrocyte populations in said sample.Type: GrantFiled: December 8, 2010Date of Patent: December 13, 2011Assignee: Litron Laboratories, Ltd.Inventor: Stephen D. Dertinger
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Patent number: 8071326Abstract: A rapid method for the quantitation of various live cell types is described. This new cell fluorescence method correlates with other methods of enumerating cells such as the standard plate count, the methylene blue method and the slide viability technique. The method is particularly useful in several applications such as: a) quantitating bacteria in milk, yogurt, cheese, meat and other foods, b) quantitating yeast cells in brewing, fermentation and bread making, c) quantitating mammalian cells in research, food and clinical settings. The method is especially useful when both total and viable cell counts are required such as in the brewing industry. The method can also be employed to determine the metabolic activity of cells in a sample. The apparatus, device, and/or system used for cell quantitation is also disclosed.Type: GrantFiled: February 11, 2011Date of Patent: December 6, 2011Assignee: Genprime, Inc.Inventors: James E. Fleming, Jason Buck Somes, Darby McLean, Jerad R. Holcomb
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Patent number: 8062860Abstract: The present invention relates a method for the enumeration of in vivo gene mutation. The method utilizes differential staining of GPI-anchor deficient erythrocyte populations to distinguish between wild-type and pig-a gene mutants. Quantitative analyses can be conducted on erythrocytes and/or reticulocytes, and is based upon fluorescent emission and light scatter following exposure to an excitatory light source. Counting of mutant erythrocytes or reticulocytes relative to the number of total erythrocytes or reticulocytes can be used to assess the DNA-damaging potential of an exogenous chemical agent, the DNA-damaging potential of an exogenous physical agent, the effects of an exogenous agent which can modify endogenously-induced DNA damage, and the effects of an exogenous agent which can modify exogenously-induced DNA damage. Kits for practicing the invention are also disclosed.Type: GrantFiled: October 6, 2010Date of Patent: November 22, 2011Assignee: Litron Laboratories, Ltd.Inventor: Stephen D. Dertinger
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Patent number: 8062609Abstract: A flow cytometer is provided which includes an interrogation flow cell and a plurality of assay fluidic lines extending into the interrogation flow cell. A method of operating such a flow cytometer includes priming the interrogation flow cell with a sheath fluid and injecting different assay fluids into a flow of the sheath fluid through the plurality of fluidic lines. A fluidic line assembly is provided which includes a plurality of capillary tubes coupled to a base section configured for coupling to an interrogation flow cell assembly of a flow cytometer. The capillary tubes are dimensionally configured such that when the fluidic line assembly is arranged within the flow cytometer and fluid is dispensed from one or more of the capillary tubes at a given pressure differential with respect to an encompassing sheath fluid within the interrogation flow cell the fluid is substantially centrally aligned within the interrogation flow cell.Type: GrantFiled: November 12, 2007Date of Patent: November 22, 2011Assignee: Luminex CorporationInventors: Jarden E. Krager, William R. Deicher, Adam Richard Schilffarth, Paul Pempsell, Wayne D. Roth
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Patent number: 8058073Abstract: An immunodiagnostic test card includes a plurality of transparent chambers wherein each chamber includes a quantity of testing material that combines with a patient sample, when mixed, to produce an agglutination reaction. A plurality of indicia are disposed to aid in the manufacture and determining the usability of the cards prior to test and also in objectively grading the agglutination reactions that are formed or lack of agglutination.Type: GrantFiled: January 30, 2008Date of Patent: November 15, 2011Assignee: Ortho-Clinical Diagnostics, Inc.Inventors: Joseph M. Chiapperi, Mary Kay Golisano
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Patent number: 8038942Abstract: A system for a clinical lab that is capable of automatically processing, including sorting, of multiple specimen containers. The system comprises a central controller, a workstation, one or more analyzers, and an automated centrifuge. The workstation has automatic detectors for detecting the presence of a holder holding specimen containers. The workstation has a bar code reader for reading bar codes on the containers. The system has a transport subsystem, preferably a workstation robotic arm and an analyzer robotic arm for transporting the specimen containers, moving them to and from the workstation, to and from the analyzers, and to and from the centrifuge. The centrifuge is loaded with buckets containing specimen containers. The workstation can be provided with a balance system for balancing the weight of the buckets used. The workstation can also have a decapper for automatically removing caps from the specimen containers.Type: GrantFiled: July 30, 2008Date of Patent: October 18, 2011Assignee: Beckman Coulter, Inc.Inventors: Wing S. Pang, Mark Gross, Hendra Tanumihardja, Ruediger F. Rauskolb, G. Andrea Pedrazzini, Santiago F. Allen
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Publication number: 20110250092Abstract: An optical material is inlaid into a supporting substrate, with the top surface of the optical material flush with the top surface of the substrate, wherein the optical element is used to shape a beam of light travelling substantially parallel to the top surface of the substrate, but with the central axis of the beam below the top surface of the substrate. The optical elements serve to shape the beam of light for delivery to or from a microfabricated structure within the device.Type: ApplicationFiled: April 7, 2010Publication date: October 13, 2011Applicant: Innovative Micro TechnologyInventors: John S. Foster, John C. Harley, Ian R. Johnston, Jeffery F. Summers
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Patent number: 8034296Abstract: A microfluidic circuit cartridge for a complete blood count, including analyses of red blood cells. Various parameters of the red blood cells may be attained. The cartridge may have sphering mechanism which has a channel or loop with a configuration for reducing or eliminating cell settling. The channel or loop may incorporate a combination of straight and curve paths in the context of gravity. The channel may alternatively have a hydrophilic or hydrophobic inside surface. Again alternatively, the channel may have an electro-wettable inside surface. Or, the channel may be subject to an electric or magnetic field. There may also be a mechanism for reducing or eliminating clumping of a sample.Type: GrantFiled: June 30, 2006Date of Patent: October 11, 2011Assignee: Honeywell International Inc.Inventors: James A. Cox, Aravind Padmanabhan, Ron L. Bardell, Christopher J. Zins
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Publication number: 20110244595Abstract: In a biomedical chip for blood coagulation tests and its manufacturing method and use, the biomedical chip comprises a substrate layer, a middle layer, and a cap layer, engaged and stacked with each other to define a microfluidic channel which has a first inlet and an outlet of the microfluidic channel respectively. A mixing interval is expanded outward from the microfluidic channel and interconnected to a second inlet, and has an interconnect portion and a capillary portion disposed between the substrate layer and the cap layer, and more specifically disposed around the periphery of the interconnect portion. With the biomedical chip having the substrate layer and cap layer made of a hydrophilic material, the blood and the reagent can be driven automatically by the capillary force of the microfluidic channel to flow and mix with each other, and the hydrophilic capillary force can be permanently maintained.Type: ApplicationFiled: March 28, 2011Publication date: October 6, 2011Applicant: National Cheng Kung UniversityInventors: Chen-Kuei Chung, Hsien-Chang Chang, Chia-Chern Chen, Yu-Sheng Chen, Cheng-Ting Li
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Patent number: 8031340Abstract: An optical system for a flow cytometer having a flow channel with an interrogation zone and an illumination source that impinges the flow channel in the interrogation zone includes a lens system and a detection system. The lens system preferably includes at least two lens surfaces located on opposite sides of the flow channel and configured to collect and collimate light from the interrogation zone. The detection system, configured to detect light from the lens system, preferably includes first and second detectors, a first filter that passes a first wavelength of light and reflects a second wavelength of light, and a second filter that reflects the first wavelength of light and passes the second wavelength of light, wherein the first and second filters are aligned such that light reflected from the first filter passes into the second detector and light reflected from the second filter passes into the first detector.Type: GrantFiled: September 21, 2010Date of Patent: October 4, 2011Assignee: Accuri Cytometers, Inc.Inventors: Collin A. Rich, Nathaniel C. Bair
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Patent number: 8029732Abstract: A quality control device for a blood analyzer using whole blood, which specifically can be used to check the correct operation of the blood analyzer. The device includes a storage for storing control bloods by cooling; a mechanism for bringing the control bloods back to the temperature specified by the control blood manufacturer; a stirring mechanism used for resuspension of the cells; and a mechanism for sampling the blood thus prepared.Type: GrantFiled: July 7, 2004Date of Patent: October 4, 2011Assignee: Horiba ABX SAInventor: Roger Le Comte
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Patent number: 8030095Abstract: A gel microdrop composition is provided. In certain embodiments, the gel microdrop composition contains a polymer matrix, an effector particle that releases an effector molecule into the polymer matrix, a first reporter particle that emits a first optically detectable signal and a second reporter particle that emits a second optically detectable signal that is distinguishable from the first optically detectable signal, where the effector particle and said first and second reporter particles are encapsulated by the polymer matrix. Methods of screening that employ the gel microdrop composition and methods of making the gel microdrop composition are also disclosed.Type: GrantFiled: March 2, 2009Date of Patent: October 4, 2011Assignee: Crystal Bioscience Inc.Inventor: William Don Harriman
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Patent number: 8026102Abstract: The present disclosure describes an apparatus and associated method for quantifying the quality degradation of individual stored red blood cell (RBC) units, thereby yielding information to improve decisions regarding their respective allocation, patient suitability, and use. This apparatus and the methods of its use are amenable to clinical implementation as well as indicative of any given unit's relative viability and thus prospective efficacy. This would provide clinicians with actual data on RBC quality when making decisions about which and how many units to use for transfusion of a given patient. Moreover, deploying this testing throughout the supply chain will improve distribution, planning, and inventory control decisions. A vital aspect of this testing system is the accumulation of copious output and other associated data and the mathematical analyses thereof to optimize algorithms by which to characterize each subsequent test output as meaningfully as possible.Type: GrantFiled: January 20, 2010Date of Patent: September 27, 2011Assignee: Blaze Medical Devices, LLCInventors: Michael Tarasev, Kenneth Alfano
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Patent number: 8021614Abstract: The invention features devices and methods for the deterministic separation of particles. Exemplary methods include the enrichment of a sample in a desired particle or the alteration of a desired particle in the device. The devices and methods are advantageously employed to enrich for rare cells, e.g., fetal cells, present in a sample, e.g., maternal blood and rare cell components, e.g., fetal cell nuclei. The invention further provides a method for preferentially lysing cells of interest in a sample, e.g., to extract clinical information from a cellular component, e.g., a nucleus, of the cells of interest. In general, the method employs differential lysis between the cells of interest and other cells (e.g., other nucleated cells) in the sample.Type: GrantFiled: June 8, 2006Date of Patent: September 20, 2011Assignees: The General Hospital Corporation, GPB Scientific, LLCInventors: Lotien Richard Huang, Thomas A. Barber, Bruce L. Carvalho, Ravi Kapur, Paul Vernucci, Mehmet Toner, Zihua Wang
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Patent number: 8021872Abstract: The present invention, provides a flow cytometry apparatus for the detection of particles from a plurality of samples comprising: means for moving a plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream; means for introducing a separation gas between each of the plurality of samples in the fluid flow stream; and means for selectively analyzing each of the plurality of samples for the particles. The present invention also provides a flow cytometry method employing such an apparatus.Type: GrantFiled: September 29, 2010Date of Patent: September 20, 2011Assignee: STC.UNMInventors: Larry A Sklar, Bruce S Edwards, Frederick W Kuckuck
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Publication number: 20110223627Abstract: A microfluidic-based flow assay and methods of manufacturing the same are provided. Specifically, the microfluidic flow assay includes a micropatterned surface that induces clot formation and an array of microfluidic channels though which blood flows. The micropatterned surface contains two clotting stimuli, one for inducing platelet adhesion and another for inducing the coagulation cascade.Type: ApplicationFiled: March 10, 2011Publication date: September 15, 2011Inventors: Keith Benjamin Neeves, Ryan R. Hansen
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Publication number: 20110223064Abstract: The present invention is a sample analyzer, comprising: a reagent storage configured to store a reagent container accommodating a reagent used for analyzing a sample; a reagent arrangement section which is accommodated in the reagent storage, and on which the reagent container is arranged; a cooling section arranged on a lower side of the reagent arrangement section; and a circulating section which is arranged between the cooling section and the reagent arrangement section and arranged to face the cooling section, and which circulates air in the reagent storage.Type: ApplicationFiled: March 10, 2011Publication date: September 15, 2011Inventors: Hironori Katsumi, Kazuya Fukuda
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Patent number: 8017402Abstract: The fluidic system of the preferred embodiment includes a sheath pump to pump sheath fluid from a sheath container into an interrogation zone and a waste pump to pump waste fluid from the interrogation zone into a waste container. The sheath pump and/or the waste pump draw sample fluid from a sample container into the interrogation zone. The fluidic system also includes a controller to adjust the flow rate of the sample fluid from the sample container into the interrogation zone. The fluidic system is preferably incorporated into a flow cytometer with a flow cell that includes the interrogation zone.Type: GrantFiled: March 8, 2006Date of Patent: September 13, 2011Assignee: Accuri Cytometers, Inc.Inventor: Collin A. Rich
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Patent number: 8012758Abstract: An apparatus and method for monitoring microbiological activity in a process stream by measuring dissolved oxygen is disclosed. Bulk microbiological activity and surface associated biological activity are measured using this apparatus and method.Type: GrantFiled: February 16, 2007Date of Patent: September 6, 2011Assignee: Nalco CompanyInventors: Michael V. Enzien, Laura E. Rice, Stephen B. Ashton
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Patent number: 8008029Abstract: The invention concerns a method for differentiating and counting cellular components present in a biological fluid sample comprising a primary cytological analysis step typically implemented by a flow cytometry equipment (1) to obtain a set of primary results enabling the set of cellular components of the sample to be differentiated and counted in different populations; and a complementary step of cytological analysis of a particular type of cellular components, based on an identified cellular peculiarity, to obtain complementary results enabling at least one cell population or subpopulation of the sample to be differentiated and counted for identification of said cellular peculiarity. The invention is applicable in particular to hematological analyses.Type: GrantFiled: July 6, 2005Date of Patent: August 30, 2011Assignee: Horiba ABX SASInventor: Didier Lefevre
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Patent number: 8003397Abstract: A polymeric sensing fluid for detecting the presence of glucose and systems and methods of its use are generally disclosed. The polymeric sensing fluid includes a polymer in a solvent (e.g., an aqueous solvent). The polymer has a plurality of boronic acid moieties extending from its polymeric backbone. As such, the polymeric sensing fluid is configured to increase in viscosity upon addition of glucose due to crosslinking between the boronic acid moieties of the polymer and glucose.Type: GrantFiled: January 29, 2009Date of Patent: August 23, 2011Assignee: University of South CarolinaInventors: Qian Wang, Siqi Li
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Patent number: 7998666Abstract: An analyte test element for determining the concentration of at least one analyte in a physiological sample fluid having a first and a second surface in a predetermined distance opposite from each other, said both surfaces are provided with two substantially equivalent patterns forming areas of high and low surface energy which are aligned mostly congruent, whereby the areas with high surface energy create a sample distribution system with at least two detection areas, characterized in that the detection areas of first and second surface are also provided with two corresponding patterns of working and reference electrodes of electrochemical detection means.Type: GrantFiled: August 13, 2004Date of Patent: August 16, 2011Assignee: Egomedical Technologies AGInventors: Matthias Stiene, Ingrid Rohm
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Patent number: 7993908Abstract: The invention relates to microscale cell separating apparatus which are able to separate cells on the basis of size of the cells, interaction of the cells with surfaces of the apparatus, or both. The apparatus comprises a stepped or sloped separation element (16) interposed between an inlet region (20) and an outlet region (22) of a void that can be filled with fluid. The void can be enclosed within a cover (12) and fluid flow through the void engages cells with the separation element. Only cells which have (or can deform to have) a characteristic dimension smaller than or equal to the distance between a step and the cover or body can pass onto or past a step. Modifications of surfaces within the apparatus can also inhibit passage of cells onto or past a step.Type: GrantFiled: July 17, 2002Date of Patent: August 9, 2011Assignee: Parsortix, Inc.Inventor: Georgi Hvichia
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Patent number: 7993865Abstract: The present invention provides a method of diagnosing Crohn's disease in a subject by determining the presence or absence or IgA anti-OmpC antibodies in the subject, where the presence of the IgA anti-OmpC antibodies indicates that the subject has Crohn's disease.Type: GrantFiled: November 17, 2006Date of Patent: August 9, 2011Assignees: Cedars-Sinai Medical Center, The Regents of the University of CaliforniaInventors: Stephan R. Targan, Jonathan Braun, Christopher L. Sutton
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Patent number: 7996188Abstract: A method of extracting and analyzing a data set from a flow cytometer system of the preferred embodiment comprises the steps of (1) running a sample and saving all collected raw data, (2) viewing raw (or “unmodified”) data, (3) modifying the raw data (e.g., scaling and/or culling the raw data), (4) reviewing and saving the modified data, and (5) exporting the saved data. Once the sample has been run and all collected data have been saved, the user can repeat the steps of modifying the raw data, saving the modified data, and exporting the saved data as many times as necessary and/or desirable.Type: GrantFiled: August 22, 2006Date of Patent: August 9, 2011Assignee: Accuri Cytometers, Inc.Inventors: David Olson, Collin A. Rich, Clement James Goebel, III
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Patent number: 7993867Abstract: The present invention provides a method of diagnosing Crohn's disease in a subject by determining the presence or absence or IgA anti-OmpC antibodies in the subject, where the presence of the IgA anti-OmpC antibodies indicates that the subject has Crohn's disease.Type: GrantFiled: January 12, 2009Date of Patent: August 9, 2011Assignees: Cedars-Sinai Medical Center, The Regents of the University of CaliforniaInventors: Stephan R. Targan, Jonathan Braun, Christopher L. Sutton
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Patent number: 7993610Abstract: A spinnable rotor for a high speed centrifuge includes a housing which defines a chamber interiorly thereof for receiving a whole blood sample and for containing a predetermined amount of red blood cell absorbent gel. The housing includes an upper portion and a bottom portion, the upper portion having a port formed through the thickness thereof. The upper portion has at least a portion thereof formed from a light transmissible material. A light pipe is joined to the upper portion and light transmissively communicates with the light transmissive portion of the upper portion. The light pipe extends at least partially into the chamber and has an open, lower free end. When whole blood filling the rotor chamber contacts the lower free end of the light pipe, the red color of the blood is transmissively communicated to the upper portion of the rotor housing where it is viewable by the user of the centrifuge.Type: GrantFiled: October 2, 2006Date of Patent: August 9, 2011Assignee: IDEXX Laboratories, IncorporatedInventor: Carl Russell Rich
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Patent number: 7993866Abstract: The present invention provides a method of diagnosing Crohn's disease in a subject by determining the presence or absence or IgA anti-OmpC antibodies in the subject, where the presence of the IgA anti-OmpC antibodies indicates that the subject has Crohn's disease.Type: GrantFiled: January 12, 2009Date of Patent: August 9, 2011Assignees: Cedars-Sinai Medical Center, The Regents of the University of CaliforniaInventors: Stephan R. Targan, Jonathan Braun, Christopher L. Sutton