Animal Or Plant Cell Patents (Class 424/93.7)
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Patent number: 11261429Abstract: This invention relates to an enriched population of isolated and expanded human cord blood stem cells and a method of producing an enriched population of isolated and expanded human cord blood stem cells. The expanded human cord blood stem cells are CD34+, CD90+, CD1 84+, CD1 17+, CD49f+, ALDH+, CD45RA? and express pluripotency genes SOX2, OCT4, NANOG, and ZIC3. In one embodiment, the stem cells in the enriched population of the present invention are positive for aldehyde dehydrogenase activity (ALDH+). In addition, in one embodiment the expanded stem cells are nearly depleted of T cells and B cells and contain a limited number of monocytes (CD14). Also disclosed is a method of treating a subject for a hematological disorder using the stem cells of the present invention and a method of determining the effects of a compound on hematopoietic stem cells.Type: GrantFiled: May 16, 2014Date of Patent: March 1, 2022Assignee: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAIInventors: Pratima Chaurasia, Ronald Hoffman
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Patent number: 11246994Abstract: Tissue product compositions and methods for treating a patient are provided. The tissue product composition may include a flowable carrier including a hyaluronic acid based material and acellular tissue matrix particles mixed within the carrier. Methods of producing the tissue product composition and an injection device filled with the tissue product composition are also provided. Methods of treating a hand of a patient are also provided.Type: GrantFiled: December 12, 2019Date of Patent: February 15, 2022Assignee: LifeCell CorporationInventors: Hui Xu, Hui Li, Ming F. Pomerleau, Darin Messina
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Patent number: 11248211Abstract: The subject invention is directed to a composition comprising primed connective tissue cells and a pharmaceutically acceptable carrier thereof.Type: GrantFiled: November 25, 2009Date of Patent: February 15, 2022Assignee: KOLON TISSUEGENE, INC.Inventors: Moon Jong Noh, Youngsuk Yi, Kwan Hee Lee
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Patent number: 11242503Abstract: A method for dissociating cell aggregates in an agitated reactor. The method comprises providing a cell culture comprising cell aggregates in the agitated reactor, contacting the cell aggregates with a dissociation reagent, generating a dissociation force in the agitated reactor and exposing the contacted cell aggregates to the generated dissociation force under conditions sufficient to dissociate the contacted cell aggregates. The method may be used in a process for passaging cells and/or generating dissociated differentiated cells from stem and/or progenitor cells.Type: GrantFiled: January 25, 2017Date of Patent: February 8, 2022Assignee: THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTOInventors: Nicholas Timmins, Lesley Chan
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Patent number: 11235005Abstract: This invention relates to a method for repairing and reconstructing a damaged or non-functional muscle, in particular to a method and a tool kit using in vitro primed motor endplate-expressing muscle progenitor cells (MPCs) to promote innervation of the damaged or non-functional muscle using an agent without any genetic manipulation. This method is particularly useful for repairing or reconstructing damaged or non-functional head and neck muscles, and urinary detrusor bladder muscle.Type: GrantFiled: April 27, 2018Date of Patent: February 1, 2022Assignee: Purdue Research FoundationInventor: Stacey L. Halum
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Patent number: 11236304Abstract: Disclosed are a method for preparing induced pluripotent stem cells from endocardium-derived adult stem cells isolated from peripheral blood and a method for differentiating induced pluripotent stem cells into cardiovascular cells. The endocardium-derived adult stem cells are primary culture cells for preparing induced pluripotent stem cells, can be readily isolated and cultured only with a small amount of peripheral blood, have a high proliferation property so as to be storable without generic variation, and can rapidly ensure a cell number so as to be usable in cell therapy. The endocardium-derived adult stem cells have sternness, thereby having high preparation efficiency, and are derived from the endocardium so as to have the epigenetic memory of cardiovascular cells, thereby having an advantage of being able to be differentiated, after preparing induced pluripotent stem cells, into cardiovascular cells such as endothelial cells, smooth muscle cells and cardiomyocytes with a high efficiency.Type: GrantFiled: November 18, 2015Date of Patent: February 1, 2022Assignee: SEOUL NATIONAL UNIVERSITY HOSPITALInventors: Hyo-Soo Kim, Han-Mo Yang, Ju-Young Kim, Joo-Eun Lee
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Patent number: 11236207Abstract: Analyte-insensitive materials comprising polymerizable monomers suitable for use in forming a hydrophilic, cross-linked gel comprising on the surface of a substrate for an electrode.Type: GrantFiled: January 21, 2020Date of Patent: February 1, 2022Inventors: Eric K. Lee, Boaz Vilozny, James Andrew
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Patent number: 11230700Abstract: Stromal stem cells are prospectively isolated from human bone marrow then expanded into clonal populations and cultured and used, the isolation being on the basis of expression of a cell surface marker, wherein the cell surface marker binds an antibody and wherein said antibody cross reacts with a cell surface marker found on mouse stromal stem cells or rat stromal stem cells, and optionally also on a cell of at least one other mammalian species selected from mouse, rat, horse, rabbit and pig cells. Useful stromal stem cell populations are positive for SDC2.Type: GrantFiled: February 11, 2013Date of Patent: January 25, 2022Assignee: ORBSEN THERAPEUTICS LIMITEDInventor: Stephen Joseph Elliman
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Patent number: 11231355Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.Type: GrantFiled: April 27, 2021Date of Patent: January 25, 2022Assignee: Bio-Rad Laboratories, Inc.Inventor: Kalyan Handique
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Patent number: 11225642Abstract: The present invention provides: a method for producing renal progenitor cells from intermediate mesoderm cells, which comprises a step of culturing intermediate mesoderm cells in a medium containing a TGF? signaling activator(s) and a BMP inhibitor(s); the renal progenitor cells produced by the method; a pharmaceutical composition comprising the renal progenitor cells; and a therapeutic drug for kidney diseases comprising the renal progenitor cells.Type: GrantFiled: June 11, 2014Date of Patent: January 18, 2022Assignees: Kyoto University, Astellas Pharma Inc.Inventors: Kenji Osafune, Takafumi Toyohara, Yukiko Yamagishi
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Patent number: 11214844Abstract: The invention herein provides biofabricated materials having zonal properties and methods of making biofabricated materials having zonal properties.Type: GrantFiled: November 13, 2018Date of Patent: January 4, 2022Assignee: Modern Meadow, Inc.Inventors: Brendan Patrick Purcell, Suzanne Lee, Lixin Dai, Katherine Amy Congdon, Stephen M. Spinella, Chi Man Ng
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Patent number: 11214772Abstract: The present invention provides a method for producing retinal cells or a retinal tissue, comprising the following steps (1)-(3): (1) a first step of culturing human pluripotent stem cells in the absence of feeder cells and in a medium comprising a factor for maintaining undifferentiated state, (2) a second step of culturing the pluripotent stem cells obtained in the first step in suspension in the presence of a Sonic hedgehog signal transduction pathway activating substance to form a cell aggregate, and (3) a third step of culturing the aggregate obtained in the second step in suspension in the presence of a 1) a BMP signal transduction pathway activating substance to obtain an aggregate containing retinal cells or a retinal tissue.Type: GrantFiled: October 23, 2015Date of Patent: January 4, 2022Assignees: SUMITOMO DAINIPPON PHARMA CO., LTD., RIKEN, SUMITOMO CHEMICAL COMPANY, LIMITEDInventors: Atsushi Kuwahara, Suguru Yamasaki, Yasushi Hiramine, Yoshiki Sasai, Masayo Takahashi
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Patent number: 11198856Abstract: The present invention relates to a method for the generation of compact Transcription Activator-Like Effector Nucleases (TALENS) that can efficiently target and process double-stranded DNA. More specifically, the present invention concerns a method for the creation of TALENs that consist of a single TALE DNA binding domain fused to at least one catalytic domain such that the active entity is composed of a single polypeptide chain for simple and efficient vectorization and does not require dimerization to target a specific single double-stranded DNA target sequence of interest and process DNA nearby the DNA target sequence. The present invention also relates to compact TALENs, vectors, compositions and kits used to implement the method.Type: GrantFiled: March 14, 2016Date of Patent: December 14, 2021Assignee: CELLECTISInventors: Philippe Duchateau, Julien Valton, Claudia Bertonati, Jean-Charles Epinat, George H. Silva, Alexandre Juillerat, Marine Beurdeley
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Patent number: 11186821Abstract: Provided is a method for inducing differentiation of neural crest cells into neurons of the autonomic nervous system, the method including the step of culturing neural crest cells in the presence of at least one of a BMP signaling pathway activator, an SHH signaling pathway inhibitor, and a Wnt signaling pathway inhibitor.Type: GrantFiled: April 26, 2016Date of Patent: November 30, 2021Assignee: National Institute of Advanced Industrial Science and TechnologyInventors: Yasuyuki Kida, Yuzo Takayama
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Patent number: 11186819Abstract: Provided is a method of serum-free culturing corneal limbal stromal stem cells and inducing them to differentiate and form spheres. Also provide is a medium combination for inducing corneal limbal stromal stem cells to form spheres or differentiate into corneal limbal stromal cells in vitro. The serum-free medium combination used herein can provide sufficient nutrients and a good environment required for cell growth and proliferation, can provide a stable in vitro expansion of corneal limbal stromal stem cells and can ensure that the expanded corneal limbal stromal stem cells keep their stemness and specificity. In addition, a system for inducing them to differentiate into corneal limbal stromal cells is successfully built. It can be used in experimental studies of corneal limbal stromal stem cells, cell therapy of corneal lesions and transplant for corneal injury.Type: GrantFiled: October 16, 2018Date of Patent: November 30, 2021Assignee: ZHONGSHAN OPHTHALMIC CENTER, SUN YAT-SEN UNIVERSITYInventors: Hong Ouyang, Liqiong Zhu
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Patent number: 11186826Abstract: An object of the present invention is to provide a cell population comprising mesenchymal cells having low cell aggregability, which is useful for intravenous administration of a cell preparation, and a method for producing the same, and a pharmaceutical composition comprising the cell population. Also provided are methods for producing a cell population comprising mesenchymal stem cells, the method comprising obtaining a cell population having the following cell characteristics (the cell population satisfies the relative expression level of COL11A1 gene to the expression level of SDHA gene of 6.0 or less and the relative expression level of COL16A1 gene to the expression level of SDHA gene of 1.5 or less).Type: GrantFiled: October 2, 2019Date of Patent: November 30, 2021Assignee: KANEKA CORPORATIONInventors: Keita Ino, Yuta Kita
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Patent number: 11178875Abstract: Methods for spray drying gram negative bacteria are provided. Such spray drying methods are performed under conditions that allow for the spray dried composition to retain the biological activity of interest of the gram-negative bacteria. Further provided are compositions comprising a spray dried gram-negative bacteria, where the spray dried formulation comprises at least 105 CFU/gram of said gram-negative bacteria. The gram-negative bacteria employed in the various methods and compositions can comprise a biological activity of interest which, in specific embodiments, controls one or more pathogens that cause plant disease and/or improve at least one agronomic trait of interest. The spray dried formulation can be used as an inoculant for plants. Methods for growing a plant susceptible to plant disease and methods and compositions for controlling plant disease are also provided. Further provided are methods and compositions of increasing disease resistance in plants.Type: GrantFiled: June 23, 2017Date of Patent: November 23, 2021Assignee: AgBiome, Inc.Inventors: Kyle Beery, Alexander P. Schlesinger, James D. Sievert, Kelly S. Smith
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Patent number: 11180728Abstract: The present disclosure concerns an in-vitro full skin model which comprises a dermal equivalent and epidermal equivalent as well as from about 1 to about 100 three-dimensional sweat gland equivalents with respectively from about 500 to about 500000 sweat gland cells as well as a diameter of respectively from about 100 to about 6000 ?m on a supporting layer. Furthermore, the present disclosure concerns the production of the full skin model as well as the use of this model as an in-vitro model, in screening methods as well as for in-vitro evaluation of the influence of cosmetic substances on the inhibition of sweat secretion as well as body odor.Type: GrantFiled: March 20, 2017Date of Patent: November 23, 2021Assignee: HENKEL AG & CO. KGAAInventors: Patricia Klaka, Sabine Gruedl, Melanie Giesen, Thomas Welss, Bernhard Banowski, Lars Vierkotten
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Patent number: 11180733Abstract: The present disclosure provides a method of generating mature mesenchymal stem cells and the cell culture medium used in such method. Also disclosed herein include a mesenchymal stem cell culture obtained by the method as disclosed herein, and uses thereof.Type: GrantFiled: July 4, 2017Date of Patent: November 23, 2021Assignee: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCHInventors: Tong Ming Liu, Bing Lim, Pin Li
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Patent number: 11168302Abstract: Provided are a method of preparing retinal ganglion cells by differentiation of stem into retinal ganglion cells, retinal ganglion cells differentiated by the method, a method of screening for a death inhibitor or a proliferation promoter of retinal ganglion cells using the retinal ganglion cells differentiated by the method, a kit of screening for the death inhibitor or the proliferation promoter of retinal ganglion cells including the retinal ganglion cells differentiated by the method, a pharmaceutical composition for treating glaucoma or optic neuropathy including the retinal ganglion cells, a method of treating glaucoma or optic neuropathy including the step of administering the retinal ganglion cells to a subject suspected of having glaucoma or optic neuropathy, and a method of preparing a mature retinal ganglion cell line.Type: GrantFiled: January 27, 2020Date of Patent: November 9, 2021Assignee: ClavisTherapeutics, INC.Inventors: Sung Sup Park, Ji Yeon Kim
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Patent number: 11166907Abstract: The present invention provides a conductive hydrogel comprising a polystyrene sulfonate compound selected from poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS), reduced graphene oxide polystyrene sulfonate (rGO:PSS) and rGO:PEDOT:PSS; and a conjugate of polyethylene glycol (PEG) and a linker-(BX)n oligopeptide of formula (I) PEG-linker-(BX)n??(I) wherein B is lysine or arginine, X is selected from alanine, glycine, serine, threonine, tyrosine, glutamic acid or aspartic acid and n is an integer selected from 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19 and 20. The invention further relates to processes for assembling the conductive hydrogel. The conductive hydrogel can be used in various biomedical applications, such as neuroprostheses, biosensors, nerve grafts, cell culture and encapsulation of cells and microorganisms as well as for drug delivery.Type: GrantFiled: April 27, 2018Date of Patent: November 9, 2021Assignee: DENOVOMATRIX GMBHInventors: Yixin Zhang, Yong Xu, Alvin Thomas
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Patent number: 11160834Abstract: Preparations comprising an enriched population of extracellular vesicles (nEVs) having a negatively charged surface, and that are CD81+ and CD9?, are provided. Improved processes and methods for producing an enriched population of nEVs from non-murine cells, especially human origin cells and/or tissues, are disclosed. Therapeutic methods for using the preparations, including for reducing brain inflammation and treatment of various pathologies associated with brain inflammation, such as by intravenous or intranasal administration, are also described. Methods and preparations for reducing brain inflammation associated with traumatic brain injury (TBI) are also disclosed. A method for treating a patient having suffered a mild traumatic injury (mTMI), or concussion, such as a sports-related head injury, is also disclosed. The nEVs are also demonstrated to reduce the expression level of IL-I? in brain tissue of an animal having had traumatic brain injury.Type: GrantFiled: April 28, 2016Date of Patent: November 2, 2021Assignee: THE TEXAS A&M UNIVERSITY SYSTEMInventors: Darwin J. Prockop, Dong-Ki Kim, Hidetaka Nishida, Askok K. Shetty
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Patent number: 11160292Abstract: An protein-polysaccharide macromolecular complex article of manufacture containing encapsulated ethyl alcohol and/or other spirits serving as an entertaining and novel non-beverage method of consuming alcohol. The article of manufacture encapsulates ethyl alcohol and/or other spirits in the amorphous regions of the complex macromolecular structure releasing it only upon chewing and exposure to the environment of the oral cavity. The article of manufacture is stable and capable of retaining shape and form for extended periods of time at ambient temperature allowing for relatively low-cost commercial manufacture and distribution.Type: GrantFiled: September 4, 2019Date of Patent: November 2, 2021Assignee: Cure Pharmaceutical Holding Corp.Inventors: Margaret Teresa Thomas Virgallito, Joshua Alan Held
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Patent number: 11161831Abstract: Embodiments of the invention include methods of treating, preventing, and/or reduce the risk or severity of a condition selected from the group consisting of muscle wasting, muscle weakness, cachexia, and a combination thereof in an individual in need thereof. In some embodiments, particular small molecules are employed for treatment, prevention, and/or reduction in the risk of muscle wasting. In at least particular cases, the small molecules are inhibitors of STAT3.Type: GrantFiled: April 14, 2020Date of Patent: November 2, 2021Assignee: Baylor College of MedicineInventors: David J. Tweardy, Moses M. Kasembeli, Marvin X. Xu, Thomas Kristian Eckols
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Patent number: 11149248Abstract: A three dimensional cell culture and bioreactor system is provided. The system comprises one or more cell culture chamber. Each cell culture chamber comprises an inlet port and an outlet port in fluid communication with the cell culture chamber. The cell culture chambers may be segregated or in fluid communication with one another. The systems may be used to conduct drug efficacy test, isolate certain cell types from a complex tissue sample of multiple cell types, allow for the ex vivo culturing of patient tissue samples to help guide the course of treatment, and conduct co-culture experiments.Type: GrantFiled: February 22, 2016Date of Patent: October 19, 2021Assignee: Kiyatec, Inc.Inventors: Matthew R. Gevaert, David E. Orr, Hal Crosswell
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Patent number: 11147840Abstract: A Muscular Dystrophy Chimeric Cell generated by ex vivo fusion of a myoblast with a second myoblast, mesenchymal stem cell, or stromal cell is described as is the use of the same in the treatment of a muscular dystrophy.Type: GrantFiled: June 10, 2016Date of Patent: October 19, 2021Assignee: THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOISInventor: Maria Siemionow
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Patent number: 11141455Abstract: The present invention relates to peptide analogues comprising one or more branched amino acid probes and a peptide, native or variants thereof.Type: GrantFiled: March 9, 2018Date of Patent: October 12, 2021Assignee: TXP Pharma GmbHInventor: Thomas Boesen
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Patent number: 11135243Abstract: The present invention provides a damaged part treatment composition for repairing a damaged part of a target tissue that includes a stem cell-conditioned medium obtained by culturing stem cells; a damaged part treatment method for repairing or restoring a damaged part of a target tissue that includes administering the damaged part treatment composition to a patient having the target tissue for the damaged part treatment composition in an amount therapeutically effective for repairing the damaged part of the target tissue; a method of treating cerebral infarction that includes administering the damaged part treatment composition to a cerebral infarct patient in an amount effective for repairing a damaged part of the brain; and a method of treating a CNS disease that includes administering, as a CNS disease treatment composition, the damaged part treatment composition to a CNS disease patient in a therapeutically effective amount.Type: GrantFiled: July 24, 2018Date of Patent: October 5, 2021Assignee: SHED Tech CorporationInventors: Minoru Ueda, Yoichi Yamada, Katsumi Ebisawa, Akihito Yamamoto, Kiyoshi Sakai, Kohki Matsubara, Hisashi Hattori, Masahiko Sugiyama, Takanori Inoue
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Patent number: 11124770Abstract: A sheet-shaped cell culture is disclosed that includes myocardial cells derived from pluripotent stem cells and has excellent functional properties. A sheet-shaped cell culture obtained by culturing a cell population that includes myocardial cells derived from pluripotent stem cells, wherein the proportion of the number of the myocardial cells derived from pluripotent stem cells to the total number of cells in the cell population is 50% to 70%; a method of producing the sheet-shaped cell culture; a composition including the sheet-shaped cell culture; and a method of treating a disease using the sheet-shaped cell culture or the composition.Type: GrantFiled: May 12, 2017Date of Patent: September 21, 2021Assignees: TERUMO KABUSHIKI KAISHA, OSAKA UNIVERSITYInventors: Hiroko Iseoka, Yoshiki Sawa, Shigeru Miyagawa, Satsuki Fukushima
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Patent number: 11124767Abstract: Many cell types in the body can remove apoptotic and cellular debris from tissues; however, the professional phagocyte, or antigen presenting cell (“APC”), has a high capacity to do so. The recognition of apoptotic cells (“ACs”) occurs via a series of evolutionarily-conserved, AC associated molecular-pattern receptors (“ACAMPRs”) on APCs that recognize and bind corresponding apoptotic-cell-associated molecular patterns (“ACAMPs”). These receptors recognize ligands such as phosphotidyl serine and oxidized lipids found on apoptotic cells. Savill et al. (2002); and Gregory et al. (2004).Type: GrantFiled: October 19, 2018Date of Patent: September 21, 2021Assignee: Mallinckrodt Pharmaceuticals Ireland LimitedInventors: David Peritt, Kim Campbell, Amy Krutsick
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Patent number: 11116799Abstract: This disclosure relates generally to new methods of maintaining the expression of hepatic genes in human hepatocytes and method for maintaining the functional hepatic enzyme activity of primary hepatocytes in culture. The disclosure also encompasses new methods of deriving a population of pure hepatocytes without selecting or sorting the cells from the cultured pluripotent cells.Type: GrantFiled: July 14, 2017Date of Patent: September 14, 2021Assignee: Wisconsin Alumni Research FoundationInventors: James A. Thomson, Srikumar Sengupta
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Patent number: 11110260Abstract: Methods and devices related to the fields of skin care, hair restoration and lip care wherein the systems may be used by an individual for infusing treatment media into skin or lips for cosmetic and rejuvenation purposes, hair restoration purposes or other therapeutic purposes.Type: GrantFiled: September 2, 2020Date of Patent: September 7, 2021Assignee: Hermes Innovations, LLCInventor: John H. Shadduck
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Patent number: 11111476Abstract: The present invention provides a method for producing a cell aggregate containing a ciliary marginal zone-like structure, including a step of culturing a cell aggregate containing a retinal tissue in which Chx10 positive cells are present in a proportion of 20% or more and 100% or less of the tissue in a serum-free medium or serum-containing medium each containing a substance acting on the Wnt signal pathway and a substance inhibiting the FGF signal pathway for only a period before the appearance of a RPE65 gene expressing cell, followed by culturing the “cell aggregate in which a RPE65 gene-expressing cell does not appear” thus obtained in a serum-free medium or serum-containing medium each free of a substance acting on the Wnt signal pathway and so on. According to the production method of the present invention, a ciliary marginal zone-like structure can be produced with high efficiency.Type: GrantFiled: February 5, 2019Date of Patent: September 7, 2021Assignees: Sumitomo Chemical Company, Limited, RIKENInventors: Atsushi Kuwahara, Yoshiki Sasai
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Patent number: 11096885Abstract: Disclosed is to provide a composition for enhancing skin elasticity or improving skin wrinkles, which comprises a polyol as a dispersion medium, and a ginseng cell lysate dispersed in the polyol as an active ingredient. Specifically, the composition according to the present disclosure contains a polyol and all useful substances in ginseng cells dispersed in a polyol as an active ingredient, thereby exhibiting excellent skin elasticity enhancement or skin winkle improvement effects. In addition, the preparing method according to the present disclosure can effectively disperse useful substances in ginseng cells in the polyol by using a micro fluidizer, and thus can exhibit synergistic effects of useful substances in ginseng cells.Type: GrantFiled: August 26, 2019Date of Patent: August 24, 2021Assignee: AMOREPACIFIC CORPORATIONInventors: Eunjung Lee, Nok Hyun Park, Hyangtae Choi, Young Gyu Kang, Dong Hyun Kim, Yong Jin Kim
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Patent number: 11091732Abstract: Methods and devices are disclosed for processing stromal precursor cells (i.e., cells which can differentiate into connective tissue cells, such as in muscles, ligaments, or tendons) which can be obtained from fatty tissue extracts obtained via liposuction. Normal processing of a liposuction extract involves centrifugation, to concentrate the stromal cells into a semi-concentrated form called “spun fat”. That “spun fat” can then be treated by mechanical processing (such as pressure-driven extrusion through 0.5 mm holes) under conditions which can gently pry the stromal cells away from extra-cellular collagen fibers and other debris in the “spun fat”. The extruded mixture is then centrifuged again, to separate a highly-enriched population of stromal cells which is suited for injection back into the patient (along with platelet cells, if desired, to further promote tissue repair or regeneration).Type: GrantFiled: May 21, 2017Date of Patent: August 17, 2021Inventor: Michael A. Scarpone
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Patent number: 11090338Abstract: The present disclosure relates generally to methods of treatment of tissue prior to implantation. In one aspect, the methods of treatment include washing adipose tissue with detergents to improve the viability of adipose cells for implantation and/or to increase the amount of viable adipose cells per volume of tissue for implantation.Type: GrantFiled: June 27, 2013Date of Patent: August 17, 2021Assignee: LifeCell CorporationInventors: Aaron Barere, Jerome Connor
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Patent number: 11090653Abstract: Microfluidic systems and methods to generate and analyze microcapsules comprising biological sample, such as for example, single cells, cellular contents, microspore, protoplast, are disclosed. The microcapsules comprising the biological sample can be preserved by a polymerization process that forms a hydrogel around the biological sample. The hydrogel microcapsules can be trapped in a trapping array or collected in an output reservoir and subject to one or more assays. The trapping array or the output reservoir can be disposed over a porous layer that can filter the continuous phase (e.g., oil) in which the microcapsules are dispersed in the microfluidic device. The pores of the porous layer are configured to be smaller than the size of the microcapsules to prevent the flow of the microcapsules through the porous layer.Type: GrantFiled: October 10, 2017Date of Patent: August 17, 2021Assignees: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Abraham P. Lee, Dohyun Lee, Yue Yun
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Patent number: 11091639Abstract: Disclosed herein are synthetic leathers, artificial epidermal layers, artificial dermal layers, layered structures, products produced therefrom and methods of producing the same.Type: GrantFiled: June 4, 2020Date of Patent: August 17, 2021Assignees: VitroLabs Inc., King's College LondonInventors: Ingvar Helgason, Dusko Ilic
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Patent number: 11085067Abstract: The present invention generally relates to a set of early developmental reference data or “lineage scorecard” for stem cells, and methods, systems and kits to generate a lineage scorecard for predicting the functionality and suitability of stem cell lines. In some aspects, methods for generating a scorecard comprises measuring the gene expression of a plurality of early developmental genes, such as pluripotent, early ectoderm, early mesoderm and early endoderm genes to predict the pluripotency and differentiation potential of the stem cell line and its functionality and/or suitability for a desired use. In some embodiments, a reference scorecard can be compared with the test stem cell line scorecard to accurately predict the utility and/or identify specific characteristics of the stem cell line, e.g., to determine its suitability for downstream applications, e.g., therapeutic use, drug screening, toxicity assays, differentiation into a desired cell lineage, etc.Type: GrantFiled: June 9, 2014Date of Patent: August 10, 2021Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGEInventors: Alexander Meissner, Alexander Tsankov, Veronika Akopian
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Patent number: 11085864Abstract: Systems and methods for uniquely identifying fluid-phase products by endowing them with fingerprints composed of dispersed colloidal particles, and by reading out those fingerprints on demand using Total Holographic Characterization. A library of chemically inert colloidal particles is developed that can be dispersed into soft materials, the stoichiometry of the mixture encoding user-specified information, including information about the host material. Encoded information then can be recovered by high-speed analysis of holographic microscopy images of the dispersed particles. Specifically, holograms of individual colloidal spheres are analyzed with predictions of the theory of light scattering to measure each sphere's radius and refractive index, thereby building up the distribution of particle properties one particle at a time. A complete analysis of a colloidal fingerprint requires several thousand single-particle holograms and can be completed in ten minutes.Type: GrantFiled: November 11, 2015Date of Patent: August 10, 2021Assignee: NEW YORK UNIVERSITYInventors: David G. Grier, David B. Ruffner, Aaron Yevick, Mark Hannel
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Patent number: 11085024Abstract: Methods are provided for extracting bone marrow cells from bone obtained from deceased donors, for preparing the bone marrow for cryopreservation and for obtaining desired cells from cryopreserved and fresh bone marrow.Type: GrantFiled: September 4, 2020Date of Patent: August 10, 2021Assignee: Ossium Health, Inc.Inventors: Erik John Woods, Brian H. Johnstone, Dongsheng Gu, Aubrey Marie Sherry, Kelsey Gwen Musall, John R. Woods, James Hardin, Alan Hooks
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Patent number: 11077182Abstract: Disclosed are methods of preparing an isolated population of human papillomavirus (HPV)-specific T cells comprise dividing an HPV-positive tumor sample into multiple fragments; separately culturing the multiple fragments; obtaining T cells from the cultured multiple fragments; testing the T cells for specific autologous HPV-positive tumor recognition; selecting the T cells that exhibit specific autologous HPV-positive tumor recognition; and expanding the number of selected T cells to produce a population of HPV-specific T cells for adoptive cell therapy. Related methods of treating or preventing cancer using the T cells are also disclosed.Type: GrantFiled: December 7, 2020Date of Patent: August 3, 2021Assignee: The United States of Americans represented by the Secretary, Department of Health and Human ServicesInventors: Christian S. Hinrichs, Steven A. Rosenberg
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Patent number: 11076984Abstract: A method includes inserting a flexible cannula between a sclera and a choroid of an eye. The needle is advanced from a distal end of the flexible cannula, such that the needle pierces the choroid to access a subretinal space of the eye. The needle is used to aspirate fluid from the subretinal space. The fluid may be present from a retinal detachment, a macular hemorrhage, or other condition present before the procedure begins. The method may further include injecting the fluid in the subretinal space via the needle, in which case the fluid may be a balanced salt solution or other bleb fluid. The method may further include injecting a therapeutic agent into the subretinal space after aspirating the bleb fluid. The volume of injected bleb fluid may be greater than the volume of injected therapeutic agent.Type: GrantFiled: March 5, 2018Date of Patent: August 3, 2021Assignee: GYROSCOPE THERAPEUTICS LIMITEDInventors: Daniel W. Price, Brendan J. Oberkircher, Daniel J. Prenger, Geoffrey King, Isaac J. Khan, Michael F. Keane, Benjamin L. Ko
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Patent number: 11066646Abstract: Methods of developing and using cell lines, such as stem cell lines, for therapeutic or cosmetic use. In one embodiment, the cell lines are used to treat a wide range of degenerative and metabolic disorders including, but not limited to, obesity, diabetes, hypertension, and cardiac deficiency. Also described are methods of using such cell lines to screen for compounds that play a role in regulating a variety of processes.Type: GrantFiled: March 2, 2018Date of Patent: July 20, 2021Assignee: BIORESTORATIVE THERAPIES, INC.Inventors: Francisco Javier Silva, Mark Weinreb, Amit N. Patel, David A. Bull
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Patent number: 11060059Abstract: The present invention provides methods for producing cell populations enriched for stable, regulatory T cells (Tregs). In particular, the invention relates to methods for culturing T cells such that the final culture is enriched for stable, regulatory T cells. It also relates to methods for stabilizing regulatory T cells. Also provided are compositions enriched for stable, regulatory T cells, which are useful for treating individuals in need of such treatment. The methods and compositions disclosed herein can also be used to treat an individual suffering from an immune-mediated disease.Type: GrantFiled: October 4, 2016Date of Patent: July 13, 2021Assignee: THE USA, AS REPRESENTED BY THE SECRETARY, DEPT. OF HEALTH AND HUMAN SERVICES NATIONAL INSTITUTES OF HEALTHInventors: Yong Chan Kim, Ethan Shevach
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Patent number: 11058721Abstract: The present invention relates to a root canal filler and a dental tissue regeneration method by using the root canal filler.Type: GrantFiled: January 15, 2018Date of Patent: July 13, 2021Inventors: Maroun Khoury, Claudia Brizuela, Ioannis Angelopoulos
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Patent number: 11059041Abstract: An in vitro microfluidic “organ-on-chip” is described herein that mimics the structure and at least one function of specific areas of the epithelial system in vivo. In particular, a multicellular, layered, microfluidic culture is described, allowing for interactions between lamina propria-derived cells and the associated tissue specific epithelial cells and endothelial cells. This in vitro microfluidic system can be used for modeling inflammatory tissue, e.g., autoimmune disorders involving epithelia and diseases involving epithelial layers. These multicellular, layered microfluidic “organ-on-chip”, e.g. “epithelia-on-chip” further allow for comparisons between types of epithelia tissues, e.g., lung (Lung-On-Chip), bronchial (Airway-On-Chip), skin (Skin-On-Chip), cervix (Cervix-On-Chip), blood brain barrier (BBB-On-Chip), etc., in additional to neurovascular tissue, (Brain-On-Chip), and between different disease states of tissue, i.e. healthy, pre-disease and diseased areas.Type: GrantFiled: September 17, 2020Date of Patent: July 13, 2021Assignee: Emulate, Inc.Inventors: S. Jordan Kerns, Riccardo Barrile, Geraldine Hamilton, Catherine Karalis, Daniel Levner, Carolina Lucchesi, Antonio Varone, Remi Villenave
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Patent number: 11052119Abstract: The present invention relates to a cell-based composition comprising a suspension of mesenchymal stem cells in crystalloid with a cellular concentration from 0.01 million to 3.0 million cells/ml. The cell-based composition is used in a form of medicament for treatment of diabetes and its associated metabolic disorders and complications.Type: GrantFiled: February 25, 2019Date of Patent: July 6, 2021Assignee: Cytopeutics Sdn. Bhd.Inventors: Bernard Sze-Piaw Chin, Kong Yong Then, Soon Keng Cheong
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Patent number: 11052175Abstract: Cartilage fibers and implants made therefrom are disclosed, with and without cartilage particles. Methods for making the cartilage fibers and the implants containing them are also disclosed. The implants may be pre-shaped and may be reshapable and provide good shape retention and little swelling when placed into a cartilage defect.Type: GrantFiled: August 15, 2016Date of Patent: July 6, 2021Assignee: Musculoskeletal Transplant FoundationInventors: Michael A. Nasert, Florence Stoffel, Paul R. Williams, Alex Callahan
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Patent number: 11045996Abstract: A method for printing uses at least one bio-ink. The method also uses at least one laser print head to deposit at least one droplet of at least one bio-ink onto a depositing surface of a receiving substrate. The printing method uses at least one nozzle print head to deposit at least one droplet of at least one bio-ink onto a depositing surface of the same receiving substrate as the laser print head.Type: GrantFiled: December 17, 2015Date of Patent: June 29, 2021Assignees: Universite de Bordeaux, Institut National de la Sante et de la Recherche Medicale—InsermInventor: Fabien Guillemot