Layer Formed Contains Chemical Reagent Or Chemically Reacts With Substrate (e.g., Cell Stain Or Fix, Ph Paper, Immobilized Antigen) Patents (Class 427/2.13)
  • Patent number: 10736992
    Abstract: The invention provides methods and compositions for making and using collagen-glycosaminoglycan three-dimensional scaffolds immobilized with biomolecules that are spatially and temporally patterned. The method comprises adding benzophenone to a collagen-glycosaminoglycan three dimensional scaffold in the dark; adding one or more biomolecules to one or more areas of the collagen-glycosaminoglycan three-dimensional scaffold (which can be done optionally in the dark or in the light); and exposing the collagen-2glycosaminoglycan three-dimensional scaffold to light at a wavelength of about 350 to about 365 nm.
    Type: Grant
    Filed: February 27, 2012
    Date of Patent: August 11, 2020
    Assignee: BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS
    Inventors: Ryan C. Bailey, Brendan A. Harley, Teresa A. Martin, Steven R. Caliari
  • Patent number: 10662227
    Abstract: The invention relates to CRISPR sequences found in bifidobacteria and their uses.
    Type: Grant
    Filed: November 6, 2009
    Date of Patent: May 26, 2020
    Assignee: DUPONT NUTRITION BIOSCIENCES APS
    Inventors: Rodolphe Barrangou, Philippe Horvath, Dennis A. Romero, Lindsay L. Traeger
  • Patent number: 10633684
    Abstract: The present invention provides an improved biotechnological production of riboflavin (also referred herein as vitamin B2) through modification in the operon containing the riboflavin biosynthetic genes (rib operon), in particular modifications of/in the leader sequences (rib leader) upstream of the corresponding riboflavin biosynthetic genes (rib operon). Furthermore, the present invention relates to genetically engineered microorganisms carrying said modified sequences, processes to generate said modified sequences/microorganisms and the use thereof for production of riboflavin.
    Type: Grant
    Filed: November 9, 2009
    Date of Patent: April 28, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Martin Lehmann, Hans-Peter Hohmann, Dietmar Laudert, Michael Hans
  • Patent number: 10359424
    Abstract: The present invention relates to an oligonucleotide, comprising a part or the entire of the nucleotide sequence shown in any of SEQ ID NO: 1 to SEQ ID NO: 15, or a part or the entire of the sequence complementary to the nucleotide sequence shown in any of SEQ ID NO: 1 to SEQ ID NO: 15, wherein the oligonucleotide is capable of hybridizing with the nucleotide sequence of Mycobacterium intracellulare (M. intracellulare) gene; a primer or a probe for the detection of M. intracellulare which comprises said oligonucleoride; and a method for detection of M. intracellulare using said primer and/or the probe.
    Type: Grant
    Filed: May 26, 2009
    Date of Patent: July 23, 2019
    Assignee: FUJIFILM WAKO PURE CHEMICAL CORPORATION
    Inventor: Tomokazu Ishikawa
  • Patent number: 10342642
    Abstract: The present invention refers to a dental implant or abutment including a ceramic body having a surface, the ceramic body including zirconia as the main component. At least a first surface area of the ceramic body is covered with an at least essentially monomolecular phosphate layer having a thickness of less than 1.0 nm. The phosphate layer contains phosphates selected from the group consisting of ortho-phosphate, poly-phosphates, cyclo-phosphates, and mixtures thereof. Furthermore, a kit including a gas- and liquid-tight container, with the dental implant or abutment being stored inside the container, and a method for the preparation of the dental implant or abutment are disclosed.
    Type: Grant
    Filed: September 23, 2013
    Date of Patent: July 9, 2019
    Assignee: STRAUMANN HOLDING AG
    Inventors: Simon Berner, Heiner Bieli
  • Patent number: 10260113
    Abstract: The present invention generally relates to a molecular test of Influenza A, Influenza B, Respiratory Syncytial Virus A, and Respiratory Syncytial Virus B in order to identify patients with a viral infection. Accordingly methods and compositions are disclosed to determine the presence or absence of a viral pathogen in a sample containing one or more target nucleic acids from the M gene of Influenza A, Influenza B, Respiratory Syncytial Virus A, and/or Respiratory Syncytial Virus B.
    Type: Grant
    Filed: December 11, 2012
    Date of Patent: April 16, 2019
    Assignee: QUEST DIAGNOSTICS INVESTMENTS INCORPORATED
    Inventors: Jules Chen, Lilly I. Kong, Ming-Chou Lee, Fan Chen, Michelle M. Tabb, Michael Aye
  • Patent number: 10232083
    Abstract: Provided are a tissue structure mimetic used for regenerating a tissue and a method for manufacturing the same, and more particularly, a 3-dimensional tissue structure mimetic which consists of a complex of extracellular matrix protein and bone mineral, wherein the complex is specifically bound to a regeneration-functional peptide to thereby be capable of implementing environment of a tissue requiring restoration, and a method for manufacturing the same. In the tissue structure mimetic according to the present invention, bone mineral components are finely dispersed in the extracellular matrix protein to have excellent mechanical strength of the tissue structure mimetic and conductivity which provides a migration path of cells involved in tissue regeneration. Further, environment of the tissue may be implemented by the peptide contained in the tissue structure mimetic to finally remarkably increase tissue regeneration capacity.
    Type: Grant
    Filed: April 1, 2014
    Date of Patent: March 19, 2019
    Assignee: NANO INTELLIGENT BIOMEDICAL ENGINEERING CORPORATION CO. LTD.
    Inventors: Chong-Pyoung Chung, Yoon Jeong Park, Jue-Yeon Lee
  • Patent number: 10195174
    Abstract: The present disclosure provides methods for detecting the presence of a cancer stem cell and their use in cancer prognosis, evaluating risk of cancer metastasis, identifying or validating drug candidates, and determining treatment efficacy. It also provides kits useful for detecting the presence of cancer stem cells as well as methods of treating cancer using CAIX inhibitors.
    Type: Grant
    Filed: October 24, 2012
    Date of Patent: February 5, 2019
    Assignees: SIGNALCHEM LIFESCIENCES CORPORATION, BRITISH COLUMBIA CANCER AGENCY BRANCH
    Inventors: Paul C. McDonald, Frances E. Lock, Shoukat Dedhar
  • Patent number: 9951341
    Abstract: The present invention relates to a nucleic acid comprising a Lactococcus CRISPR repeat region and/or a Lactococcus CRISPR spacer region.
    Type: Grant
    Filed: October 20, 2011
    Date of Patent: April 24, 2018
    Assignee: DUPONT NUTRITION BIOSCIENCES APS
    Inventors: Philippe Horvath, Dennis Romero, Anne M. Millen
  • Patent number: 9947538
    Abstract: A method for manufacturing a semiconductor device may include the following steps: preparing a semiconductor structure that comprises a substrate and a first fin member, wherein the first fin member is connected to the substrate and comprises a first semiconductor portion; providing a first-type dopant member that directly contacts the first semiconductor portion, comprises first-type dopants, and is at least one of liquid and amorphous; and performing heat treatment on at least one of the first-type dopant member and the first semiconductor portion to enable a first portion of the first-type dopants to diffuse through a first side of the first-type dopant member into the first semiconductor portion.
    Type: Grant
    Filed: March 10, 2016
    Date of Patent: April 17, 2018
    Assignees: SEMICONDUCTOR MANUFACTURING INTERNATIONAL (SHANGHAI) CORPORATION, SEMICONDUCTOR MANUFACTURING INTERNATIONAL (BEIJING) CORPORATION
    Inventor: Yong Li
  • Patent number: 9845494
    Abstract: The invention relates to methods for enzymatic, genotyping of polymorphisms on solid supports. In some aspects the methods include ligation of allele or base specific interrogation probes to an array probe. The array probe is labeled by ligation of the interrogation probe. Ligation is dependent on the identity of the base immediately adjacent to the end of the array probe. In other aspects array bound probes are labeled by template dependent extension.
    Type: Grant
    Filed: October 18, 2007
    Date of Patent: December 19, 2017
    Assignee: Affymetrix, Inc.
    Inventors: Glenn K. Fu, Keith W. Jones
  • Patent number: 9790398
    Abstract: The present application is directed at the reaction product of an azide compound having two or more azide groups attached thereto and an alkyne compound having two or more alkyne groups attached thereto, wherein the azide and alkyne groups react in a 1,3-dipolar cyclo addition to form 1,4-disubstituted triazols and wherein the azide or alkyne compound or both include —O—(C?O)—NR— functional groups. The reaction products can be used as coatings, such as for flat roofs, sealants, adhesives and in elastomer applications. Methods for producing the reaction products as well as substrates including a coating of the reaction product are also disclosed.
    Type: Grant
    Filed: February 5, 2014
    Date of Patent: October 17, 2017
    Assignee: SIKA TECHNOLOGY AG
    Inventors: Neil Carter, Ian Martin, Alexander Coward, Mark Gatrell, Michael Byrne, John E. Cullen, Peter Cormack
  • Patent number: 9644257
    Abstract: A method of fabricating a composite PDMS microstructure includes a defining step, a depositing step and an etching step. The defining step is performed for defining a patterned area having a mono-molecule with a thiol group on a PDMS substrate, and the mono-molecule with the thiol group is in liquid phase. The depositing step is performed for placing the PDMS substrate having the mono-molecule with the thiol group into a vacuum chamber within an activation time so as to deposit one Au atom on the patterned area of the PDMS substrate by a vacuum coating process. The etching step is performed for cleaning the PDMS substrate using water, and thus the Au atom can be selectively retained on the patterned area of the PDMS substrate.
    Type: Grant
    Filed: August 18, 2015
    Date of Patent: May 9, 2017
    Assignee: NATIONAL TSING HUA UNIVERSITY
    Inventors: Fan-Gang Tseng, Pen-Cheng Wang, Tung-Yua Lee, Chun-Ting Lin
  • Patent number: 9550047
    Abstract: A balloon catheter, in particular a balloon catheter for angioplasty, the balloon having an inner or outer surface modified by means of a photoactivation reaction.
    Type: Grant
    Filed: September 27, 2011
    Date of Patent: January 24, 2017
    Assignee: Invatec S.P.A.
    Inventors: Simone D'Onofrio, Paolo Pellegrini
  • Patent number: 9540695
    Abstract: Described herein are compositions and methods for the prediction of the prognosis of ovarian cancer subjects. The present invention further provides methods for distinguishing between histological subtypes of ovarian cancer tumors, and also methods and compositions for the treatment or prevention of ovarian cancer. Specifically the invention relates to microRNA molecules associated with said methods and compositions, as well as various nucleic acid molecules relating thereto or derived therefrom.
    Type: Grant
    Filed: May 19, 2009
    Date of Patent: January 10, 2017
    Assignees: Rosetta Genomics Ltd., MOR Research Applications
    Inventors: Moshe Hosen, Gila Lithwick Yanai, Ram Eitan
  • Patent number: 9353415
    Abstract: The present invention relates generally to groups of genes that can be used to diagnose and differentiate between types of specific diseases such as breast cancer. The groups of genes can be further used to develop diagnostic kits for the specific diseases. The diagnostic kits can also differentiate between sub-categories of a disease to help identify the appropriate treatment regimen for a patient.
    Type: Grant
    Filed: December 19, 2007
    Date of Patent: May 31, 2016
    Assignee: Thomson Reuters (Scientific) LLC
    Inventors: Yuri Nikolsky, Andrej Bugrim, Tatiana Nikolskaya
  • Patent number: 9315870
    Abstract: The present invention relates to a method for detecting methylation of the bowel-cancer-specific methylation marker GPM6A (NM_201591, glycoprotein M6A) gene in order to diagnose bowel cancer, and more specifically relates to a method for providing information for diagnosing bowel cancer by detecting the methylation of a bowel-cancer-specific marker gene that is specifically methylated in bowel cancer cells. The method for detecting methylation and a diagnostic composition, kit and nucleic-acid chip according to the present invention can be used to advantage in diagnosing bowel cancer more accurately and quickly than by normal methods as they permit bowel cancer to be diagnosed at the initial genetic transformation step and so allow early diagnosis.
    Type: Grant
    Filed: December 16, 2011
    Date of Patent: April 19, 2016
    Assignee: GENOMICTREE, INC.
    Inventors: Sung Whan An, Tae Jeong Oh
  • Patent number: 9302679
    Abstract: Control panels with input elements for controlling components corresponding to each of the controls. The input elements can further be actuated by detecting an optical reflection associated with an input element.
    Type: Grant
    Filed: December 29, 2011
    Date of Patent: April 5, 2016
    Assignee: INTEL CORPORATION
    Inventor: David L. Graumann
  • Patent number: 9287114
    Abstract: The invention provides a method for chemically modifying a surface of a substrate, preferably a silicon substrate, including the steps of providing a substrate having at least a portion of a surface thereof coated with an organic coating composition including unsaturated moieties forming a surface coating, and introducing a vapor phase reactive intermediate species based on a Group 14 or Group 15 element from the Periodic Table of Elements to the substrate whereupon the reactive intermediate species is able to react with a number of the unsaturated moieties in the coating composition thereby chemically modifying the surface coating. Also disclosed is a surface-modified substrate obtained or obtainable by the method, and uses thereof in the fabrication of MEMS and IC devices.
    Type: Grant
    Filed: August 15, 2012
    Date of Patent: March 15, 2016
    Assignee: University Court of the University of St Andrews
    Inventors: Georg Haehner, Malgorzata Adamkiewicz, David O'Hagan
  • Patent number: 9260762
    Abstract: Methods for detecting Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp. are disclosed. A sample suspected of containing a nucleic acid of one or more of M. pneumoniae, C. pneumoniae, and Legionella spp. is screened for the presence or absence of that nucleic acid. Determining whether the M. pneumoniae, C. pneumoniae, or Legionella spp. nucleic acid is present in the sample can be accomplished by detecting hybridization between a M. pneumoniae probe (such as a CARDS toxin probe), a C. pneumoniae probe (such as a ArgR probe), or a Legionella spp. probe (such as a SsrA probe) and a nucleic acid in a sample. Also disclosed are probes and primers for the detection of M. pneumoniae, C. pneumoniae, and Legionella spp., and kits that contain the disclosed probes and/or primers.
    Type: Grant
    Filed: April 15, 2011
    Date of Patent: February 16, 2016
    Assignee: The United States of America as represented by the Secretary of the Department of Health and Human Services, Centers for Disease Control and Prevention
    Inventors: Jonas M. Winchell, Agnes Warner, Kathleen Thurman
  • Patent number: 9116087
    Abstract: The systems and methods disclosed herein permit automated preparation of biological specimens for examination. The disclosed systems and methods provide fast, efficient, and highly uniform specimen processing using minimal quantities of fluids. The methods include at least a fixing phase for fixing a biological specimen to a substrate such as a microscope slide, a staining phase for staining the specimen, and a rinsing phase for rinsing the specimen. One or more of the fixing, staining, and rinsing phases include one or more agitation cycles for distributing reagents evenly and uniformly across the specimen. The systems can be implemented as a standalone device or as a component in a larger system for preparing and examining biological specimens.
    Type: Grant
    Filed: April 8, 2013
    Date of Patent: August 25, 2015
    Assignee: Roche Diagnostics Hematology, Inc.
    Inventors: Daniel Lapen, David Zahniser, Mark Licari, Brian J. McKeen, Eric D. Yeaton, Dennis Poole
  • Patent number: 9102983
    Abstract: Methods for determining the genetic predisposition of a human subject to developing renal disease, such as focal segmental glomerulosclerosis (FSGS) or end-stage kidney disease are provided herein. These methods include methods for detecting renal disease, or determining the risk of developing renal disease in a human subject, such as a subject of African ancestry. The methods utilize the detection of one or more haplotype blocks comprising at least two tag single nucleotide polymorphisms (SNPs) in a non-coding region of a MYH9 gene or detecting the presence of at least one tag SNP in a non-coding region of a MYH9 gene. An array for detecting a genetic predisposition to renal disease using probes complementary to the tag SNPs in the non-coding region of the MYH9 gene are also disclosed.
    Type: Grant
    Filed: January 30, 2009
    Date of Patent: August 11, 2015
    Assignee: The United States of America as represented by the Secretary, Department of Health and Human Services
    Inventors: Cheryl Winkler, George Nelson, Jeffrey B. Kopp, Michael W. Smith, Randall Johnson
  • Patent number: 9068283
    Abstract: In one aspect, the present invention relates to a layered structure usable in a strain sensor. In one embodiment, the layered structure has a substrate with a first surface and an opposite, second surface defining a body portion therebetween; and a film of carbon nanotubes deposited on the first surface of the substrate, wherein the film of carbon nanotubes is conductive and characterized with an electrical resistance. In one embodiment, the carbon nanotubes are aligned in a preferential direction. In one embodiment, the carbon nanotubes are formed in a yarn such that any mechanical stress increases their electrical response. In one embodiment, the carbon nanotubes are incorporated into a polymeric scaffold that is attached to the surface of the substrate. In one embodiment, the surfaces of the carbon nanotubes are functionalized such that its electrical conductivity is increased.
    Type: Grant
    Filed: October 29, 2012
    Date of Patent: June 30, 2015
    Assignee: BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS
    Inventors: Alexandru S. Biris, Steven Trigwell, Walter Hatfield
  • Patent number: 9063144
    Abstract: Provided are a detection method and a quantification method for a detection target, which can detect and quantify the detection target rapidly, inexpensively, simply, and with high accuracy in a variety of environments.
    Type: Grant
    Filed: May 21, 2010
    Date of Patent: June 23, 2015
    Assignees: JNC Corporation, Ortho-Clinical Diagnostics Kabushiki Kaisha
    Inventors: Katsuhiko Kitsugi, Satoru Sugita, Noriyuki Ohnishi
  • Patent number: 9044493
    Abstract: The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of an Adiponectin (ADIPOQ), in particular, by targeting natural antisense polynucleotides of an Adiponectin (ADIPOQ). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of Adiponectins (ADIPOQ)s.
    Type: Grant
    Filed: August 11, 2010
    Date of Patent: June 2, 2015
    Assignee: CuRNA, Inc.
    Inventors: Joseph Collard, Olga Khorkova Sherman
  • Publication number: 20150129425
    Abstract: An enzyme electrode includes an electrode, and a detection layer that is in contact with the electrode and includes an oxidoreductase, a water-soluble conductive polymer, and conductive particles, electrons being transferred between the enzyme and the electrode by direct electron transfer in the detection layer.
    Type: Application
    Filed: June 25, 2013
    Publication date: May 14, 2015
    Applicant: BIOENGINEERING LABORATORIES, LLC
    Inventors: Masashi Tsukada, Mayumi Tajima
  • Publication number: 20150110882
    Abstract: It is intended to provide a stable metallic nanostructure that causes no aggregation when surface-modified with biomolecule-reactive functional molecules. 30 to 90% of the surface of a metallic nanostructure is covered with at least one or more types of colloid-stabilizing functional molecules. The remaining portions on the surface of the metallic nanostructure are further covered with one or more types of biologically functional molecules.
    Type: Application
    Filed: October 18, 2013
    Publication date: April 23, 2015
    Applicants: JAPANESE FOUNDATION FOR CANCER RESEARCH, IMRA America, Inc.
    Inventors: Yuki Ichikawa, Kiyotaka Shiba
  • Publication number: 20150104561
    Abstract: A test sensor reagent for measuring the concentration of analytes in body fluids includes cellulose polymers for improving the stability of the test sensor and reducing the total assay time. The test sensor reagent also includes an enzyme, an electron transfer mediator and a rheological additive.
    Type: Application
    Filed: December 18, 2014
    Publication date: April 16, 2015
    Inventors: Amy H. Chu, Andrew J. Edelbrock, Hope G. Spradlin
  • Publication number: 20150050196
    Abstract: A colorimetric time indicator configured to indicate time specificity is provided. The colorimetric time indicator includes a colorimetric layer configured to be operatively coupled to a disposable medical device. The colorimetric time indicator is configured to indicate the time specificity via one or more visual indicators upon exposure to one or more external stimuli.
    Type: Application
    Filed: August 14, 2013
    Publication date: February 19, 2015
    Applicant: General Electric Company
    Inventors: Kwok Pong Chan, Sumeet Jain, Matthew Jeremiah Misner, Pekka Simeon Astola, Johannes Harri Eemeli Osterberg
  • Patent number: 8945944
    Abstract: A device for quantitatively determining an analyte is provided to conspicuously improve the performance of the quantitative determination. This device is equipped with a flow channel, an analyte detecting unit for capturing and detecting the analyte, and a quantitative measurement unit for quantitatively determining the analyte, wherein a signal generated when the analyte detecting unit has detected the analyte is divided into a plurality of parts in the direction of the flow in the flow channel at the quantitative measurement unit for processing. Also provided are technologies including one for controlling the density of molecules attached to the surface of a solid. In these technologies, when molecules are attached to a substrate, the density of attached molecules is controlled, by having an electrolyte also present in a solution containing the molecules to adjust the screening effect by the electrolyte, and by taking into consideration the effective size of a molecule.
    Type: Grant
    Filed: September 29, 2010
    Date of Patent: February 3, 2015
    Assignee: Fujitsu Limited
    Inventors: Kenji Arinaga, Ulrich Rant
  • Publication number: 20150017318
    Abstract: Provided is a method for fabricating a microchip for nucleic acid amplification reaction that is capable of simple and highly accurate analysis. Provided is a method for fabricating a microchip for nucleic acid amplification reaction, the method including a solidification step of drying a reagent solution including at least a part of substances required for a nucleic acid amplification reaction, and a containment step of arranging the reagent solution including the solidified substance in wells that serve as a reaction site for a nucleic acid amplification reaction. In the microchip for nucleic acid amplification reaction fabricated by the fabrication method, since substances required for the nucleic acid amplification reaction are contained by being solidified, non-specific amplification is suppressed in a nucleic acid amplification reaction, which enables highly accurate analysis.
    Type: Application
    Filed: January 16, 2013
    Publication date: January 15, 2015
    Applicant: Sony Corporation
    Inventors: Masahiro Matsumoto, Masaki Sato, Hidetoshi Watanabe
  • Publication number: 20150017067
    Abstract: There is provided a fluid test strip, comprising a micro-sensor formed on a substrate surface and a reagent deposition site formed on the substrate surface, operate to control the size and location of a reagent deposition. There is also provided a method of forming fluid test strip, comprising forming a micro-sensor on a substrate surface and forming a reagent deposition site on the substrate surface, operable to control the size and location of a reagent deposition.
    Type: Application
    Filed: February 15, 2013
    Publication date: January 15, 2015
    Inventors: Robert Ibbotson, Vladislav Djakov, Richard Dunn
  • Publication number: 20150001073
    Abstract: An analyte sensor for the continuous or semi-continuous monitoring of physiological parameters and a method for making the analyte sensor are disclosed. In one aspect, the analyte sensor includes a crosslinked, hydrophilic copolymer in contact with a surface of an electrode, and an analyte sensing component embedded within the crosslinked, hydrophilic copolymer. The crosslinked, hydrophilic copolymer has methacrylate-derived backbone chains of first methacrylate-derived units, second methacrylate-derived units and third methacrylate-derived units. The first and second methacrylate-derived units have side chains that can be the same or different, and the third methacrylate-derived units in different backbone chains are connected by hydrophilic crosslinks.
    Type: Application
    Filed: June 28, 2013
    Publication date: January 1, 2015
    Inventors: Zenghe Liu, Jeffrey George Linhardt
  • Publication number: 20150001074
    Abstract: An analyte sensor for the continuous or semi-continuous monitoring of physiological parameters and a method for making the analyte sensor are disclosed. In one aspect, the analyte sensor includes an electrode, a sensing layer in contact with a surface of the electrode, and a protective membrane. The sensing layer is a crosslinked, hydrophilic copolymer including poly(alkylene oxide) and poly(vinyl pyridine), and an analyte sensing component is immobilized within the crosslinked, hydrophilic copolymer. The protective membrane is a crosslinked, hydrophilic copolymer including alkylene oxide, vinyl pyridine and styrene units. The method involves the formation of a sensing layer on a surface of an electrode, followed by the formation of a protective membrane on a surface of the sensing layer.
    Type: Application
    Filed: June 28, 2013
    Publication date: January 1, 2015
    Inventor: Zenghe Liu
  • Publication number: 20140356252
    Abstract: A biomedical testing sheet comprises: a substrate with calligraphy paper material, wherein the substrate comprises a wax pattern layer, which is a part of a surface of the substrate coated by a waxy material, and penetrated and diffused by the waxy material, and the wax pattern layer comprises one or more carrying units for carrying one or more droplets of liquids to be measured. The lotus effect substantially prevents diffusion of the liquids and reagents on the carrying units, and reduces the required amount of liquids to be measured and the amount of agent.
    Type: Application
    Filed: May 6, 2014
    Publication date: December 4, 2014
    Applicant: National Tsing Hua University
    Inventors: Chen-Meng Kuan, Chao-Min Cheng, Kuan-Hung Chen
  • Publication number: 20140349005
    Abstract: Disclosed herein are biosensor systems and related methods for detecting analytes in aqueous and biologic environments. A biosensor system for detecting binding of an analyte of interest may include a detector configured to detect a change in an electrical property on a surface thereof. The detector may be a FET. The system also may include a passive layer disposed on a top surface of the detector. Further, the system may include a hydrophobic layer disposed on the passive layer. The system also may include a receptor-attachment material configured for binding to an analyte. A receptor may bind to the analyte, and the receptor may be attached to the receptor-attachment material. The binding of the analyte to the receptor can cause the change of the electrical property at the surface. In response to the change for example, a current may change for indicating the binding of the analyte to the receptor.
    Type: Application
    Filed: December 3, 2012
    Publication date: November 27, 2014
    Applicant: THE JOHNS HOPKINS UNIVERSITY
    Inventors: Allen Dale Everett, Howard Katz, Kalpana Besar, Weiguo Huang
  • Publication number: 20140273027
    Abstract: A non-treponemal diagnostic test for syphilis infection includes initially dissolving cholesterol in an organic solvent and further diluting the dissolved cholesterol in an ethanol solution comprising cardiolipin and lecithin, permitting a volume of the antigen solution to evaporate in place within a container, rinsing the coated container with buffered saline, stabilizing the antigen coating by overcoating the antigen coating with an inert protein dissolved in buffered saline, decanting the overcoat solution, air-drying the container, and sealing the container in vapor-proof pouches with desiccant, providing an enzyme-labeled conjugate component of a syphilis infection test that is formulated to be compatible with a lipid nature of the cholesterol, the cardiolipin, and the lecithin VDRL antigens, providing a sample diluent that is formulated to be compatible with the lipid nature of the VDRL antigens, and providing a wash fluid that is formulated to be compatible with the lipid nature of the VDRL antigens.
    Type: Application
    Filed: February 26, 2014
    Publication date: September 18, 2014
    Applicant: Awareness Technology Inc.
    Inventors: David J. Kiefer, Oscar R. Compain
  • Publication number: 20140262777
    Abstract: The present invention relates to analytical testing devices and methods for fabricating electrochemical creatinine biosensors, and in particular using point of care electrochemical biosensors for testing for creatinine in samples. For example, the present invention may be directed to a biosensor having an electrode, a first printed layer formed on the electrode and having a first matrix that includes creatinine amidohydrolase (CNH), creatine amidinohydrolase (CRH), and sarcosine oxidase (SOX), and second printed layer formed over the first printed layer and having a second matrix that includes CRH, SOX, and catalase.
    Type: Application
    Filed: March 12, 2014
    Publication date: September 18, 2014
    Applicant: Abbott Point of Care Inc.
    Inventors: Tian-Xian Zhao, Glenn Martin, Kenneth Hardage, Steven R. Breeze, Stephen Snyder
  • Publication number: 20140251826
    Abstract: Biosensors and biosensor systems are disclosed that have manganese (III) oxide (Mn2O3)-based electrodes that can attenuate interference of a detection signal resulting from an analyte-relevant reaction caused by undesired reaction of interferents in a sample. Methods are also disclosed for making and using the same.
    Type: Application
    Filed: March 6, 2014
    Publication date: September 11, 2014
    Applicant: Roche Diagnostics Operations, Inc.
    Inventors: Karl-Heinz Koelker, Arnulf Staib
  • Patent number: 8808787
    Abstract: The formation in quantity of various different populations of a substance being studied with multiple combinations of distribution form and distribution density by dripping a suspension of a single concentration of the substance onto a masking member of a certain specified structure placed on a substrate by making use of the sedimentation of said substance.
    Type: Grant
    Filed: August 20, 2009
    Date of Patent: August 19, 2014
    Assignee: Japan Science and Technology Agency
    Inventors: Koji Ikuta, Masashi Ikeuchi
  • Patent number: 8802183
    Abstract: The system of the present invention includes a conductive element, an electronic component, and a partial power source in the form of dissimilar materials. Upon contact with a conducting fluid, a voltage potential is created and the power source is completed, which activates the system. The electronic component controls the conductance between the dissimilar materials to produce a unique current signature. The system can also measure the conditions of the environment surrounding the system.
    Type: Grant
    Filed: July 11, 2011
    Date of Patent: August 12, 2014
    Assignee: Proteus Digital Health, Inc.
    Inventors: Jeremy Frank, Peter Bjeletich, Hooman Hafezi, Robert Azevedo, Robert Duck, Iliya Pesic, Benedict Costello, Eric Snyder
  • Patent number: 8802024
    Abstract: A method for manufacturing a biochip is provided. First, a first self-assembled monolayer is coated on a substrate. Next, a plurality of first biomedical molecular dots are formed on the first self-assembled monolayer by micro-titration technique. After the bonding reaction between the first biomedical molecular point and the first self-assembled monolayer, a second self-assembled monolayer is deposited on the surface of the first self-assembled monolayer by evaporation. The second self-assembled monolayer attached on the plurality of first biomedical molecular dots and the first self-assembled monolayer not bonded to the substrate are removed, so that the first biomedical molecular dots immobilized on the first self-assembled monolayer are exposed. Finally, a second biomedical molecular layer is immobilized on the exposed portions of the first biomedical molecular dots.
    Type: Grant
    Filed: September 28, 2007
    Date of Patent: August 12, 2014
    Assignee: National Tsing Hua University
    Inventors: Fan Gang Tseng, Hsin Yi Hsieh, Chun Lung Wu
  • Publication number: 20140147933
    Abstract: The invention relates to a method of coating beads with a biological molecule comprising: (i) coating a plurality of beads with the biological molecule; (ii) mixing the coated beads with a liquid stabilising and/or blocking agent (iii) dispersing the coated beads still substantially surrounded by a liquid phase comprising the liquid stabilising and/or blocking agent across a surface that is at least partially liquid permeable; (iv) drying the beads on the surface to substantially remove the liquid phase; and (v) removing the dried beads from the surface.
    Type: Application
    Filed: October 26, 2011
    Publication date: May 29, 2014
    Applicant: THE BINDING SITE GROUP LIMITED
    Inventors: Roger Budd, David Taylor
  • Patent number: 8733557
    Abstract: The present invention relates to a substrate including a compound having blood anticoagulation activity and a hydrophilic polymer compound, wherein the amount of elution of the compound having blood anticoagulation activity is less than 0.6 ?g/ml, and a manufacturing method of a substrate, wherein after a compound having blood anticoagulation activity and a hydrophilic compound brought in contact with a substrate are irradiated with radiation, unreacted components are washed out.
    Type: Grant
    Filed: September 13, 2007
    Date of Patent: May 27, 2014
    Assignee: TORAY Industries, Inc.
    Inventors: Hirokazu Sakaguchi, Hiroshi Takahashi, Yoshiyuki Ueno, Hiroyuki Sugaya
  • Patent number: 8734890
    Abstract: A method for forming a molecularly imprinted polymer biosensor includes: (a) preparing a reaction solution including an imprinting molecule, a functional monomer, an initiator, and a crosslinking agent; (b) disposing the reaction solution in a space between upper and lower substrates each of which is made of a light-transmissible material; (c) disposing on the upper substrate a photomask having a patterned hole; (d) irradiating the reaction solution through the patterned hole of the photomask and the upper substrate so that the reaction solution undergoes polymerization to form a polymer between the upper and lower substrates; (e) removing the upper substrate after the polymer is formed on the lower substrate; and (f) extracting the imprinting molecule from the polymer so that a patterned molecularly imprinted polymer film is formed on the lower substrate.
    Type: Grant
    Filed: September 24, 2010
    Date of Patent: May 27, 2014
    Assignee: National Tsing Hua University
    Inventors: Chien-Chong Hong, Po-Hsiang Chang, Chih-Chung Lin
  • Publication number: 20140141484
    Abstract: The invention relates to compositions and methods for the immunoassay of an analyte of interest. The analyte is detected in an immunoassay using three or more antibodies, wherein each antibody specifically binds to a different epitope on the analyte. When the analyte of interest in a clinical marker for an acute disease, the detection of the analyte by immunoassay is a diagnosis of the occurrence of the disease.
    Type: Application
    Filed: November 15, 2013
    Publication date: May 22, 2014
    Applicant: Abbott Point of Care Inc.
    Inventors: John Lewis Emerson Campbell, Pamela Anne Frank, Meghan Elizabeth Hawkes, Shannon Reishma Lobin, Cary James Miller, Zhen Yang
  • Patent number: 8728510
    Abstract: A biocompatible carrier for delivery of a therapeutic substance or an active agent is disclosed. The carrier contains a bioadhesive material allowing for increased residence time of the active agent at the treatment site.
    Type: Grant
    Filed: March 15, 2002
    Date of Patent: May 20, 2014
    Assignee: Advanced Cardiovascular Systems, Inc.
    Inventor: Wouter E. Roorda
  • Publication number: 20140134652
    Abstract: Systems and methods are disclosed for improved detection sensitivity of assays. The systems include an upper plate that is inserted into a well of testing well, such as a base microtiter plate. For bottom detection, a coating, including polymer coatings or membrane coatings, is applied to an insert portion of the upper plate, and the base plate includes transparent windows. In bottom detection, a detector will image from below the base plate. Alternatively, for top detection, the coating is applied to the well of the base plate, and the upper plate includes transparent windows. In top detection, a detector will image from above the upper plate. Analysis features, including capture antibody features or antigen features, can be printed on the coating surface for forward-phase assays or reverse-phase assays, respectively. Methods for making and using the same are disclosed.
    Type: Application
    Filed: November 8, 2013
    Publication date: May 15, 2014
    Applicant: AUSHON BIOSYTEMS, INC.
    Inventors: Peter HONKANEN, Scott DOUGLAS
  • Patent number: 8673643
    Abstract: A closed loop automated method for staining of a biological sample is provided. The method comprises providing a biological sample, staining at least a portion of the biological sample by flowing in a reagent, monitoring one or more optical characteristics of the biological sample, and calculating a figure of merit based on at least one of the optical characteristics. An automated device for iterative staining of a biological sample is also provided.
    Type: Grant
    Filed: November 30, 2010
    Date of Patent: March 18, 2014
    Assignee: General Electric Company
    Inventors: Alex David Corwin, Robert John Filkins, Jun Xie, Christopher James Sevinsky, Kashan Ali Shaikh
  • Patent number: 8673386
    Abstract: Disclosed herein is a composition for glucose sensing obtained by dispersing in a solvent such as acetone a nanofibrous membrane fabricated by electrospinning a mixture containing poly(vinylidene fluoride) and poly(aminophenylboronic acid). Also disclosed is a method of fabricating a non-enzymatic glucose biosensor based on an electrospun nanofibrous membrane by depositing the composition on an electrode.
    Type: Grant
    Filed: October 24, 2008
    Date of Patent: March 18, 2014
    Assignee: Kyungpook National University Industry Academic Cooperation Foundation
    Inventors: Kwang Pill Lee, Iyengar Gopalan Anantha, Sundaram Komathi Shanmuga