Proteinaceous Coating Patents (Class 427/338)
  • Patent number: 9150751
    Abstract: The present invention relates to a composition comprising a stable aqueous dispersion of crosslinkable polymer particles, a peroxidase enzyme, and a hydrophobic hydroperoxide having a 10-hour half life temperature of at least 100° C. The composition provides a robust one-pot peroxidase mediated cure for latex particles bearing a variety of polymerizable functional groups.
    Type: Grant
    Filed: July 28, 2014
    Date of Patent: October 6, 2015
    Assignee: Rohm and Haas Company
    Inventor: Selvanathan Arumugam
  • Patent number: 9005707
    Abstract: In order to achieve an object of applying easily producible and variable patterns effectively and in widely varying ways, lacquer is applied to a decoration target material (1) comprising a transparent or translucent glass or resin material to form a lacquer layer (3), and thereafter an alcohol agent is mixed into the undried lacquer layer (3) to make the lacquer material flow and to form lacquer patterns (3a, 3b) using the flow behavior of the lacquer, or a crack pattern is formed on, for example, a foil layer (7), thereby to significantly reduce the drying time of the lacquer layer (3), and to suitably form a lacquer pattern comprising a flowing lacquer pattern, a lacquer crack pattern, and a foil crack pattern.
    Type: Grant
    Filed: April 27, 2010
    Date of Patent: April 14, 2015
    Assignee: Duco Co., Ltd.
    Inventor: Kazuko Ohori
  • Patent number: 8932823
    Abstract: The disclosure relates to methods, medical profiles, kits and apparatus for use in determining the risk that a pregnant individual has for developing pre-eclampsia based on amounts of certain biochemical markers in a biological sample from the individual and biophysical markers. The disclosure also relates to methods, medical profiles, kits and apparatus for use in determining the risk that a pregnant individual is carrying a fetus having a chromosomal abnormality based on amounts of certain biochemical markers in a biological sample from the individual and biophysical markers.
    Type: Grant
    Filed: December 31, 2013
    Date of Patent: January 13, 2015
    Assignees: Wallac Oy, The Fetal Medicine Foundation
    Inventors: Howard Cuckle, Kypros Nicolaides
  • Patent number: 8921283
    Abstract: Methods and apparatuses for generating microscopic patterns of macromolecules such as proteins on a solid surface are described. Pulsed laser light is used to alter surface portions of a solid surface substrate in a predetermined pattern, by removing macromolecules from surface portions of the substrate where the light is focused. The same wavelength light at lower intensity can be used to visualize the removal by its reflection from the specimen surface along the path to the detector. Select macromolecules introduced to the substrate selectively adhere to select surface portions, thereby depositing macromolecules in the predetermined pattern on the solid surface.
    Type: Grant
    Filed: October 30, 2007
    Date of Patent: December 30, 2014
    Assignee: Washington University
    Inventors: Paul Bridgman, Stephen Turney
  • Patent number: 8790882
    Abstract: The present invention relates to the field of immunology and hyperproliferative diseases. More specifically, the present invention relates to a method of detecting and monitoring therapeutic antibody:antigen complex, soluble antigen and soluble therapeutic antibody, wherein a patient has undergone at least one course of immunotherapy. Yet further, levels of therapeutic antibody:antigen complexes, soluble antigens or soluble therapeutic antibodies may be measured and used to stage or monitor a hyperproliferative disease.
    Type: Grant
    Filed: July 3, 2013
    Date of Patent: July 29, 2014
    Assignee: Board of Regents, The University of Texas System
    Inventors: Maher Albitar, Michael J. Keating, Taghi Manshouri
  • Patent number: 8673582
    Abstract: The disclosure relates to methods, medical profiles, kits and apparatus for use in determining the risk that a pregnant individual has for developing pre-eclampsia based on amounts of certain biochemical markers in a biological sample from the individual and biophysical markers. The disclosure also relates to methods, medical profiles, kits and apparatus for use in determining the risk that a pregnant individual is carrying a fetus having a chromosomal abnormality based on amounts of certain biochemical markers in a biological sample from the individual and biophysical markers.
    Type: Grant
    Filed: July 18, 2013
    Date of Patent: March 18, 2014
    Assignees: Wallac Oy, The Fetal Medicine Foundation
    Inventors: Howard Cuckle, Kypros Nicolaides
  • Publication number: 20140057053
    Abstract: Polypeptides are provided, where the polypeptides include one or more groups of formula —R1—C(O)R2, where R1 is an amino acid side chain, R2 is a C8-C24 polyunsaturated alkenyl group, and the polypeptide is a gelatin.
    Type: Application
    Filed: August 27, 2012
    Publication date: February 27, 2014
    Inventors: William B. Carlson, Vincenzo Casasanta, III, Gregory David Phelan
  • Patent number: 8658384
    Abstract: The present invention relates to combinations of biomarkers and levels thereof that may be used, for example, in the determination of risk associated with the occurrence of a major adverse cardiac event (MACE) in a patient.
    Type: Grant
    Filed: June 25, 2013
    Date of Patent: February 25, 2014
    Assignee: Abbott Laboratories
    Inventors: Saul A. Datwyler, Jessie W. Shih, Robert C. Doss, Walter J. Keirans, Sudarshan Hebbar
  • Patent number: 8574858
    Abstract: The present disclosure provides immunoassays and kits for detection or quantification of an analyte of interest in a test sample that potentially contains endogenously produced autoantibodies reactive with the analyte.
    Type: Grant
    Filed: October 2, 2012
    Date of Patent: November 5, 2013
    Assignee: Abbott Laboratories
    Inventors: Maciej Adamczyk, Roy Jeffrey Brashear, Phillip G. Mattingly
  • Patent number: 8568993
    Abstract: A diagnostic method for determining the absence or presence of a disease is provided. The method includes assaying the amount and/or types of glycopeptides in a sample from a subject, and comparing these to the amount and types of reference glycopeptides. The method may include the use of a stable isotope label, affinity selection, immunoaffinity chromatography, and glycoproteomics techniques, to identify and quantify changes in glycosylated peptides or glycosylated proteins associated with cancers such as malignant lymphoma or breast cancer, to monitor patient's response to therapy, and to monitor disease recurrence.
    Type: Grant
    Filed: June 30, 2008
    Date of Patent: October 29, 2013
    Assignee: Purdue Research Foundation
    Inventors: Fred E. Regnier, Stephen B. Hooser, Christina R. Wilson, Wonryeon Cho
  • Patent number: 8501420
    Abstract: The present invention relates to combinations of biomarkers and levels thereof that may be used, for example, in the determination of risk associated with the occurrence of a major adverse cardiac event (MACE) in a patient.
    Type: Grant
    Filed: August 30, 2010
    Date of Patent: August 6, 2013
    Assignee: Abbott Laboratories
    Inventors: Saul A. Datwyler, Jessie W. Shih, Robert C. Doss, Walter J. Keirans, Sudarshan Hebbar
  • Patent number: 8501423
    Abstract: The present invention relates to the field of immunology and hyperproliferative diseases. More specifically, the present invention relates to a method of detecting and monitoring therapeutic antibody:antigen complex, soluble antigen and soluble therapeutic antibody, wherein a patient has undergone at least one course of immunotherapy. Yet further, levels of therapeutic antibody:antigen complexes, soluble antigens or soluble therapeutic antibodies may be measured and used to stage or monitor a hyperproliferative disease.
    Type: Grant
    Filed: July 6, 2012
    Date of Patent: August 6, 2013
    Assignee: Board of Regents, The University of Texas System
    Inventors: Maher Albitar, Michael J. Keating, Taghi Manshouri
  • Patent number: 8232068
    Abstract: The present invention relates to the field of immunology and hyperproliferative diseases. More specifically, the present invention relates to a method of detecting and monitoring therapeutic antibody:antigen complex, soluble antigen and soluble therapeutic antibody, wherein a patient has undergone at least one course of immunotherapy. Yet further, levels of therapeutic antibody:antigen complexes, soluble antigens or soluble therapeutic antibodies may be measured and used to stage or monitor a hyperproliferative disease.
    Type: Grant
    Filed: January 12, 2012
    Date of Patent: July 31, 2012
    Assignee: Board of Regents, The University of Texas System
    Inventors: Maher Albitar, Michael J. Keating, Taghi Manshouri
  • Publication number: 20120148850
    Abstract: Sorption reinforced catalytic coating system for the degradation of threat agents including a synzyme coating about a material, the synzyme coating having bucket-shaped molecules for the sorption and degradation of the threat agents. A binding agent is configured for synzyme immobilization to maximize loading and retention of the synzyme coating on the material.
    Type: Application
    Filed: June 17, 2010
    Publication date: June 14, 2012
    Inventors: Yongwoo Lee, Tomasz Modzelewski, John P. Puglia, Steven E. Weiss, Robert A. Reinstein
  • Patent number: 8183002
    Abstract: The present disclosure provides immunoassays and kits for detection or quantification of an analyte of interest in a test sample that potentially contains endogenously produced autoantibodies reactive with the analyte.
    Type: Grant
    Filed: December 3, 2009
    Date of Patent: May 22, 2012
    Assignee: Abbott Laboratories
    Inventors: Maciej Adamczyk, Jeffrey R. Brashear, Phillip G. Mattingly
  • Patent number: 8124359
    Abstract: A method for reducing non-specific binding in an assay is provided herein. The method includes (a) providing a reaction mixture, which includes or is suspected to include a first component and a second component capable of binding to each other in a specific binding reaction, and (b) adding non-physiological amounts of at least one additive to the reaction mixture before, during or after binding in a sufficient amount to reduce non-specific binding in the reaction mixture. The method further includes (c) monitoring or measuring the presence and/or concentration of at least one of the first and second components after step (b).
    Type: Grant
    Filed: March 16, 2007
    Date of Patent: February 28, 2012
    Assignee: Aokin AG
    Inventors: Stuart Levison, Paul Nix, Derek Levison
  • Patent number: 8114616
    Abstract: The invention provides an ELISA assay for the determination of serum mast cell ?-tryptase levels using rabbit anti-tryptase as the capture antibody and alkaline phosphatase conjugated G3 as the detecting antibody. Luminescent substrate CPSD was used to enhance the assay sensitivity. Also provided are methods for aiding in the diagnosis of irritable bowel syndrome by detecting the serum level of ?-tryptase, histamine and/or prostaglandin E2.
    Type: Grant
    Filed: August 24, 2010
    Date of Patent: February 14, 2012
    Assignee: Prometheus Laboratories Inc.
    Inventors: Hua Gong, Shui Long Wang, Sharat Singh
  • Patent number: 8114615
    Abstract: The invention provides an improved method for identifying and interpreting tissue specimens and/or cells derived from tissue specimens. A panel of cell-based reagents provides a number of readouts of cellular states or biomarkers that together define a profile of a diversity of cellular states or biomarkers in a tissue specimen representing the “systems” nature of biology. This cellular profile is interpreted using informatics tools, to identify similarities between specimens, in vivo medical conditions, and suggest options for treating medical conditions.
    Type: Grant
    Filed: May 17, 2007
    Date of Patent: February 14, 2012
    Assignee: Cernostics, Inc.
    Inventors: Albert H. Gough, Kenneth A. Giuliano, D. Lansing Taylor
  • Patent number: 8114661
    Abstract: The present invention is directed to the detection of target analytes using electronic techniques, particularly AC techniques.
    Type: Grant
    Filed: May 4, 2010
    Date of Patent: February 14, 2012
    Assignee: Osmetech Technology, Inc.
    Inventors: Stephen D. O'Connor, Jon Faiz Kayyem, Thomas J. Meade
  • Patent number: 8114618
    Abstract: The present invention relates to the field of immunology and hyperproliferative diseases. More specifically, the present invention relates to a method of detecting and monitoring therapeutic antibody:antigen complex, soluble antigen and soluble therapeutic antibody, wherein a patient has undergone at least one course of immunotherapy. Yet further, levels of therapeutic antibody:antigen complexes, soluble antigens or soluble therapeutic antibodies may be measured and used to stage or monitor a hyperproliferative disease.
    Type: Grant
    Filed: April 5, 2011
    Date of Patent: February 14, 2012
    Assignee: Board of Regents of the University of Texas System
    Inventors: Maher Albitar, Michael J. Keating, Taghi Manshouri
  • Patent number: 8110365
    Abstract: Renal injury and inflammation is diagnosed by detecting an elevation in GSK3b level or activity. Inflammation of bodily tissues such as renal tissue is inhibited by administration of GSK3b inhibitory compositions.
    Type: Grant
    Filed: March 29, 2007
    Date of Patent: February 7, 2012
    Assignee: Rhode Island Hospital
    Inventors: Lance Dworkin, Rujun Gong
  • Patent number: 8110366
    Abstract: The invention describes specific sialylated structures present on human stem cells and cell populations derived thereof. The invention is especially directed to methods to control the status of stem cells by observing changes in sialylation of the cells; and control of potential contaminations of biological materials; and reagents and methods used in connection with the cells in order to avoid alterations of the cell glycosylation by contaminating materials. The invention is further directed to novel stem cells, the glycosylation of which has been specifically altered.
    Type: Grant
    Filed: July 7, 2006
    Date of Patent: February 7, 2012
    Assignees: Suomen Punainen Risti, Veripalvelu, Glykos Finland Oy
    Inventors: Jarmo Laine, Taina Jaatinen, Heidi Anderson, Johanna Nystedt, Sarl Tiitinen, Anita Laitinen, Ulla Impola, Tero Satomaa, Jari Natunen, Annamari Heiskanen, Maria Blomqvist, Anne Olonen
  • Patent number: 8105788
    Abstract: Methods, systems, kits and assays for diagnosing, monitoring or screening for mucopolysaccharidosis (MPS) and methods and systems for assaying test compounds for therapeutic activity are described, whereby MPS marker protein levels are assayed as an indication of MPS.
    Type: Grant
    Filed: December 8, 2006
    Date of Patent: January 31, 2012
    Assignee: The University of British Columbia
    Inventors: Lorne A. Clarke, Derrick Raymond Randall, Graham Sinclair
  • Patent number: 8097427
    Abstract: The invention relates to a method of using high mass matrix assisted laser desorption-ionization (MALDI) mass spectrometry for the qualitative and quantitative analysis of the effect of drug candidates on protein complexes such as protein-protein interactions in purified samples or complex biological matrices, as well as to the use of this method for lead compound optimization, drug characterization, drug manufacturing processes, and drug quality control processes, including automated high throughput applications.
    Type: Grant
    Filed: March 14, 2008
    Date of Patent: January 17, 2012
    Assignee: CovalX AG
    Inventors: Alexis Txomin Nazabal, Ryan Jay Wenzel
  • Patent number: 8093005
    Abstract: A method of preparing a protein-resistant reactive solid support surface is disclosed. The method comprises the steps of providing a solid support having a hydrogel coating with a plurality of binding elements, coupling a protein resistant compound to the hydrogel via a first fraction of the binding elements, and coupling at least one binding agent to the hydrogel via a second fraction of the binding elements, whereby the protein resistant compound and the at least one binding agent are co-immobilized to the hydrogel. Also the use of the reactive surface in analysis, such as immunogenicity assays, is disclosed.
    Type: Grant
    Filed: October 19, 2009
    Date of Patent: January 10, 2012
    Assignee: GE Healthcare Bio-Sciences AB
    Inventors: Tanja Jarhede, Per Kjellin, Anita Larsson, Hans Sjobom
  • Patent number: 8039038
    Abstract: This invention provides a process for the preparation of protein mediated calcium hydroxyapatite (HAp) coating on metal substrates particularly on stainless steel (316 L) by biomimetic route, capable of rapid and effective osteointegration with the host tissue following controlled interfacial reactions. For the purpose, calcium deficient, carbonated hydroxyapatite (HAp) coating was developed on metal substrates particularly on stainless steel (316L) alloy through biomimetic route after a surface treatment step using aqueous solution (4-10 wt %) of BSA at room temperature. The coating was characterized with respect to phase composition, crystallinity, morphology and thickness. The protein mediated calcium phosphate ceramic coating is porous (pore dia—100-200 ?m) having uniform pore distribution and coverage. There is multifold enhancement in thickness and crystallinity (data provided) of the as-prepared coating.
    Type: Grant
    Filed: July 18, 2008
    Date of Patent: October 18, 2011
    Assignee: Council of Scientific and Industrial Research
    Inventors: Chakraborty Jui, Bharati Sanghamitra, Sinha Mithlesh Kumar, Basu Debabrata
  • Publication number: 20110212501
    Abstract: Embodiments of the invention provide three dimensional multi-layered hydrogel constructs with embedded channels, living cells and bioactive agents, and methods for making three dimensional multi-layered hydrogel constructs. The constructs can have bioactive agents to support the living cells. The multi-layered constructs can have channels for perfusion purposes and layers of different hydrogel materials.
    Type: Application
    Filed: September 14, 2009
    Publication date: September 1, 2011
    Applicant: THE BRIGHAM AND WOMEN'S HOSPITAL, INC.
    Inventor: Seung-Schik Yoo
  • Publication number: 20110124079
    Abstract: Sorption reinforced catalytic coating system for the degradation of threat agents including a synzyme coating about a material configured for the degradation of the threat agents, the synzyme coating including bucket-shaped molecules configured for the sorption of the threat agents A binding agent is configured for synzyme immobilization to maximize loading and retention of the synzyme coating on the material.
    Type: Application
    Filed: June 17, 2010
    Publication date: May 26, 2011
    Inventors: Yongwoo Lee, Tomasz Modzelewski, John P. Puglia, Steven E. Weiss, Robert A. Reinstein
  • Publication number: 20110008543
    Abstract: A biodegradable biopolymer material consists of silk fibroin from domesticated silkworm; silk fibroin from wild silkworm; a composite material comprising silk fibroin from domesticated silkworm and silk fibroin from wild silkworm; or a composite material comprising either silk fibroin from domesticated silkworm or silk fibroin from wild silkworm and at least one secondary substance selected from the group consisting of cellulose, chitin, chitosan, chitosan derivatives, keratin from wool and polyvinyl alcohol. The material may be prepared by, for instance, casting an aqueous solution of domesticated silkworm silk fibroin on the surface of a substrate and then cast drying the applied solution. The biodegradable biopolymer material is effectively used as, for instance, a metal ion-adsorbing material, a sustained release substrate for a useful substance such as a medicine, a biological cell-growth substrate and a biodegradable water-absorbing material.
    Type: Application
    Filed: September 9, 2010
    Publication date: January 13, 2011
    Applicant: NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES
    Inventors: Masuhiro TSUKADA, Takayuki ARAI
  • Patent number: 7556862
    Abstract: Wood finishes and methods of production and application of wood finishes are provided. The finishes may contain biologically produced components, such as proteins, and specifically may include whey proteins derived from dairy processing. The wood finishes are environmentally friendly as they use reduced levels of solvents and provide a safe and protective coating for wood and wood products.
    Type: Grant
    Filed: January 18, 2005
    Date of Patent: July 7, 2009
    Assignee: The University of Vermont and State Agricultural College
    Inventors: Mingruo Guo, Nareen Wright, Jiancai Li
  • Patent number: 7501289
    Abstract: It is an object of the present invention to provide a biosensor, which is not significantly affected by the baseline fluctuation and suppresses nonspecific adsorption. The present invention provides a biosensor, which comprises a metal surface or metal film coated with a hydrophobic polymer, and has two or more types of different surfaces in a region coated with a hydrophobic polymer.
    Type: Grant
    Filed: December 27, 2004
    Date of Patent: March 10, 2009
    Assignee: FUJIFILM Corporation
    Inventors: Toshiaki Kubo, Toshihide Ezoe
  • Patent number: 7323224
    Abstract: A method for discharging a liquid containing a biological material includes adding as a dispense stabilizer a polymer composed of phosphorylcholine-group-containing unsaturated compound units or a copolymer including same to the liquid containing the biological material; and discharging the liquid containing the dispense stabilizer using an inkjet system.
    Type: Grant
    Filed: December 14, 2005
    Date of Patent: January 29, 2008
    Assignees: Seiko Epson Corporation, Kazuhiko Ishihara
    Inventors: Fumio Takagi, Kazuhiko Ishihara
  • Patent number: 7256259
    Abstract: The present invention is a method for a covalent ligation of one or more molecules to one or more surfaces, that is site-specific and both rapid and high yielding. The covalent ligation to the surface is based on the reaction of an azide and a phosphinothioester to form an amide bond. The method of the invention is particularly well-suited to the immobilization of peptides, proteins or protein fragments to surfaces.
    Type: Grant
    Filed: August 30, 2004
    Date of Patent: August 14, 2007
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Ronald T. Raines, Matthew B. Soellner
  • Patent number: 7108891
    Abstract: A method of making a microarray comprising the steps of: providing a support; coating on the support a receiving layer to receive microspheres, the receiving layer being capable of undergoing sol/gel transition; coating on the receiving layer a dispersion of microspheres in a carrier fluid, wherein the carrier fluid contains at least one crosslinking agent and is capable of solvating the receiving layer; allowing the microspheres to partially submerge into the receiving layer; creating conditions to induce sol/gel transition in the receiving layer, thus immobilizing the microspheres; evaporating off the carrier fluid; and allowing crosslinking reaction between the receiving layer and the crosslinker in the carrier fluid.
    Type: Grant
    Filed: March 7, 2002
    Date of Patent: September 19, 2006
    Assignee: Eastman Kodak Company
    Inventors: Krishnan Chari, Tiecheng A. Qiao, Ramasubramaniam Hanumanthu
  • Patent number: 6767928
    Abstract: Disclosed are advantageous methods for patterning and/or mineralizing biomaterial surfaces. The techniques described are particularly useful for generating three-dimensional or contoured bioimplant materials with patterned surfaces or patterned, mineralized surfaces. Also provided are various methods of using the mineralized and/or patterned biomaterials in tissue engineering, such as bone tissue engineering, providing more control over ongoing biological processes, such as mineralization, growth factor release, cellular attachment and tissue growth.
    Type: Grant
    Filed: March 17, 2000
    Date of Patent: July 27, 2004
    Assignee: The Regents of the University of Michigan
    Inventors: William L. Murphy, Martin C. Peters, David J. Mooney, David H. Kohn
  • Patent number: 6767544
    Abstract: The present invention provides for methods of treating cardiovascular diseases in a mammal. The methods include a step of administering an effective amount of a botulinum toxin directly to a blood vessel of a mammal thereby treating a cardiovascular disease.
    Type: Grant
    Filed: April 1, 2002
    Date of Patent: July 27, 2004
    Assignee: Allergan, Inc.
    Inventors: Gregory F. Brooks, Stephen Donovan
  • Patent number: 6756234
    Abstract: It has been found that casein and salts of casein are useful as replacements for, or in addition to, BSA as materials for coating solid phases, particularly magnetic particles, used for immunoassays and other binding assays for separation of the desired analyte. By using casein, immunoassays having improved stability and few discordant samples have been developed. Casein used at a concentration of 0.05-4.0 grams per gram of paramagnetic particle (optimally approximately 0.78-1.2 grams of casein per gram of magnetic particle) has been found to confer this benefit. In addition, a process for coating solid phases has been invented, said process comprising the mixing of casein with magnetic particles at 30-60° C. for 5-180 hours, said process resulting in casein-coated paramagnetic particles which either (1) already have combined therewith active ingredients needed in the binding assay or (2) are capable of reacting with active ingredients needed in the binding assay.
    Type: Grant
    Filed: December 21, 2001
    Date of Patent: June 29, 2004
    Assignee: Bayer Corporation
    Inventors: Wei-Chao Ni, Daniel W. Eustace, Steve Chin-Shen Chang
  • Patent number: 6692790
    Abstract: The invention relates to a method for providing a proteinaceous coating on a medical implant, comprising the steps of: submersing the implant in a first aqueous solution comprising a protein and magnesium, calcium and phosphate ions through which a gaseous weak acid is passed; degassing the solution; allowing a coating to precipitate onto the implant; submersing the coated implant in a second solution to redissolve the magnesium, calcium and phosphate ions and to obtain the proteinaceous coating.
    Type: Grant
    Filed: August 5, 2002
    Date of Patent: February 17, 2004
    Assignee: Chienna B.V.
    Inventors: Yuelian Liu, Klaas de Groot, Pierre Jean F. Layrolle
  • Patent number: 6669981
    Abstract: Methods of enhancing the photostabilizing of silver in medical materials are described. More particularly, the methods increase the photostabilization of silver in certain materials comprising hydrophilic, amphoteric and anionic polymers by subjecting the polymers to solutions containing an organic solvent and silver, during or after which one or more agents are added which facilitate the photostablization of the material.
    Type: Grant
    Filed: November 29, 2001
    Date of Patent: December 30, 2003
    Assignee: Bristol-Myers Squibb Company
    Inventors: David Parsons, Elizabeth Jacques, Philip Bowler
  • Patent number: 6579847
    Abstract: Method and apparatus of vascular neuromuscular blockade including percutaneous delivery of a quantity of neurotransmitter inhibiting agent to an affected region of a vessel.
    Type: Grant
    Filed: May 1, 2000
    Date of Patent: June 17, 2003
    Assignee: Imarx Therapeutics Inc.
    Inventor: Evan C. Unger
  • Patent number: 6544796
    Abstract: A system for producing multiple diagnostic test elements has a support (1) with an analytical region (3) to which diagnostic test spots (8) are applied, one or several stop edges (11-13) for each direction in space for positioning the support, a first holding unit (10I) into which the support is inserted and positioned by means of the one or more stop edges, a first printing head (20I) arranged above the holding unit (10) for applying drops of a first liquid to the analytical region of the support (1), a first positioning unit which laterally displaces and positions the first holding unit, a second holding unit (10II) into which the support is inserted and positioned by means of the one or more stop edges, a second printing head (20II) arranged above the second holding unit for applying drops of a second liquid to the analytical region of the support (1), a second positioning unit which laterally displaces and positions the second holding unit, a transport unit (41) and a control unit.
    Type: Grant
    Filed: March 31, 2000
    Date of Patent: April 8, 2003
    Assignee: Roche Diagnostics GmbH
    Inventors: Udo Eichenlaub, Martin Masch
  • Patent number: 6468667
    Abstract: The invention relates to an ophthalmic molding such as a contact lens or any kind of ocular prostheses comprising an organic bulk material having covalently bonded to its surface an acceptor saccharide to which is enzymatically attached one or more further carbohydrates. The ophthalmic moldings of the invention have desirable characteristics regarding, for example, adherence to the substrate, durability, hydrophilicity, wettability or biocompatibility.
    Type: Grant
    Filed: June 30, 2000
    Date of Patent: October 22, 2002
    Assignee: Novartis AG
    Inventors: Peter Chabrecek, Dieter Lohmann, Markus Streiff
  • Patent number: 6461874
    Abstract: It has been found that casein and salts of casein are useful as replacements for, or in addition to, BSA as materials for coating solid phases, particularly magnetic particles, used in immunoassays and other binding assays for separation of the desired analyte. By using casein, immunoassays having improved stability and fewer discordant samples have been developed. Casein used at a concentration of 0.05-4.0 grams per gram of paramagnetic particle (optimally approximately 0.78-1.2 grams of casein per gram of magnetic particle) has been found to confer this benefit.
    Type: Grant
    Filed: January 26, 2000
    Date of Patent: October 8, 2002
    Assignee: Bayer Corporation
    Inventors: Wei-Chao Ni, Daniel W. Eustace, Steve Chin-Shen Chang
  • Patent number: 6417009
    Abstract: A linker lipid for use in attaching a membrane including a plurality of ionophores to an electrode and providing a space between the membrane and the electrode in which the membrane is either in part or totally made up of the linker lipid. The linker lipid has within the same molecule a hydrophobic region capable of spanning the membrane, an attachment group used to attach the molecule to an electrode surface, a hydrophilic region intermediate the hydrophobic region and the attachment group, and a polar head group region attached to the hydrophobic region at a site remote from the hydrophilic region. The attachment group has a cross sectional area greater than the cross sectional area of the hydrophilic region, and has the structure recited in the specification.
    Type: Grant
    Filed: March 8, 1999
    Date of Patent: July 9, 2002
    Assignees: Australian Membrane and Biotechnology Institute, The University of Sydney
    Inventors: Burkhard Raguse, Christopher John Burns, Leslie David Field, Damon Donald Ridley
  • Patent number: 6358753
    Abstract: The present invention relates to a method for binding antibody or antigen molecules to solid phase. The reactive compound comprising an antibody or antigen molecule and a solide phase are formed by coupling a specific antibody or antigen molecule to a solid phase using a two-step reaction. The coupling is covalent, between the active groups of the solid phase and the antibody, a fragment thereof or the antigen molecule, in the presence of an anionic surfactant, after adding an alkaline/solution to raise the pH to the basic region.
    Type: Grant
    Filed: June 16, 1999
    Date of Patent: March 19, 2002
    Assignee: Orion-Yhtymä Oyj
    Inventors: Juhani Luotola, Martti Malassu
  • Patent number: 6342346
    Abstract: Electrode membrane combinations for use in biosensors to detect analytes in a sample and methods for making and storing same are disclosed. In one aspect, a method is provided for producing a first layer electrode membrane comprising: (1) Forming a solution containing Linker Lipid A, the disulfide of mercaptoacetic acid (MAAD) or similar molecule, linker Gramicidin B, membrane spanning lipid C (MSL-C) and membrane spanning lipid D (MSL-D) or other suitable linker molecules and other ion channel combinations; (2) Contacting an electrode containing a clean gold surface with the solution, the disulfide containing components in the solution thus adsorbing onto the gold surface of the electrode; (3) Rinsing the electrode with a suitable organic solvent; and (4) Removing the excess organic solvent used for rinsing.
    Type: Grant
    Filed: March 4, 1999
    Date of Patent: January 29, 2002
    Assignee: Australian Membrane and Biotechnology Research Institute
    Inventors: Burkhard Raguse, Ronald John Pace, Lionel George King, Vijoleta Lucija Braach-Maksvytis, Bruce Cornell
  • Patent number: 6261809
    Abstract: The invention relates to a method for marking solid, liquid or gaseous substances, whereby the substance to be marked is provided with at least one synthetically produced nucleic acid sequence. Said nucleic acid sequence contains a first sequence section constructed with the 5′ terminal end, a second sequence comprised of at least two bases and connected to said nucleic acid sequence, and a third sequence section constructed with the 3′ terminal end and connected to the said nucleic acid sequence. In order to simplify the identification of the marking, the invention provides that a first primer group is used with a first primer section corresponding to the first sequence section and a second primer group is used with a third primer section corresponding to the third sequence section.
    Type: Grant
    Filed: March 3, 2000
    Date of Patent: July 17, 2001
    Assignee: november Aktiengesellschaft Gesellschaft fur Molekulare Medizin
    Inventors: Wolf Bertling, Hans Kosak
  • Patent number: 6149977
    Abstract: A method of crosslinking an oxidative polymer having oxidatively crosslinkable functional groups and a two-pack composition used therein is provided. The oxidatively crosslinkable functional groups on the oxidative polymer are crosslinked by contacting the oxidative polymer with a catalytic amount of an oxidizing enzyme, such as horseradish peroxidase. The present invention is further directed to a two-pack coating composition which includes a polymeric component and a catalytic component, which are mixed together prior to use.
    Type: Grant
    Filed: April 6, 1999
    Date of Patent: November 21, 2000
    Assignee: Rohm and Haas Company
    Inventors: Gordon Charles Fischer, Gary Robert Larson
  • Patent number: 6150181
    Abstract: The invention relates to a process for producing ferrofluid-forming magnetic particles substituted by DMSA (FFSH) that can be covalently coupled, directly or through a difunctional reactant, to an effector, which process comprises combining DMSA with ferrofluid-forming magnetic particles to form FFSS particles having disulfide bonds, and reducing the disulfide bonds of the FFSS at basic pH to form FFSH. The invention includes a composition comprising such FFSH magnetic particles. The invention also relates to a method in which FFSH magnetic particles are incubated with an effector comprising at least one functional group which forms an S--S, C--S, C--C, or C--N bond with DMSA, so that a covalent effector-FFSH complex is formed. The invention further includes a method for separating and/or distinguishing between molecules or cells having a ligand that forms an affinity complex with an effector-FFSH particle of the invention, and those molecules or cells that do not have such a ligand.
    Type: Grant
    Filed: June 11, 1998
    Date of Patent: November 21, 2000
    Assignee: Universite Pierre et Marie Curie
    Inventors: Avraham Halbreich, Domagoj Sabolovic, Claude Sestier, Danielle Geldwerth, Jean-Noel Pons, Jacky Roger
  • Patent number: 6133047
    Abstract: The superparamagnetic monodispersed particles comprise a core of a first polymer, an internal layer of a second polymer coating the core and in which a magnetic material is distributed, and an external layer of a third polymer coating the magnetic layer and capable of interacting with at least one biological molecule. At least the second polymer is heat sensitive and has a predetermined lower critical solubility temperature (LCST) of 15-65.degree. C. These particles may be used to isolate at least one biological molecule from a liquid specimen.
    Type: Grant
    Filed: January 15, 1998
    Date of Patent: October 17, 2000
    Assignee: Bio Merieux
    Inventors: Abdelhamid Elaissari, Christian Pichot, Bernard Mandrand, Florence Sauzedde