Disaccharide Patents (Class 435/100)
  • Patent number: 11028314
    Abstract: Interactions between aqueous fluids and clay-containing subterranean formations may be problematic due to issues associated with clay destabilization and migration. Subterranean treatment methods may comprise: providing a clay stabilizing composition comprising an amine-functionalized dextrin compound having 2 to about 20 glucose units linked together with ?(1,4) glycosidic bonds, and an amine-functionalized dextran polymer having a plurality of glucose units linked together with ?(1,6) glycosidic bonds; introducing the clay stabilizing composition into a subterranean formation bearing a clay-containing mineral; and interacting the amine-functionalized dextrin compound and the amine-functionalized dextran polymer with the clay-containing mineral to affect stabilization thereof. A portion of the glucose units are oxidatively opened and functionalized with at least one amine group at a site of oxidative opening in both the amine-functionalized dextrin compound and the amine-functionalized dextran polymer.
    Type: Grant
    Filed: July 11, 2019
    Date of Patent: June 8, 2021
    Assignee: Integrity Bio-Chemicals, LLC
    Inventors: Christopher P. Gardner, Kristopher Megahan, Curtis J. Rodencal, Charles R. Landis, Ashoka V. R. Madduri
  • Patent number: 10918732
    Abstract: Certain embodiments are directed to method for synthesizing and using glycoconjugates based on the immunodominant epitope Gal?(1,3)Gal?(1,4)GlcNAc? (Gal?3LN?).
    Type: Grant
    Filed: July 26, 2019
    Date of Patent: February 16, 2021
    Assignee: THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: Igor C. Almeida, Katja Michael, Nathaniel Schocker, Susana Portillo, Rosa Maldonado
  • Patent number: 10822602
    Abstract: The present invention provides a ?-agarase, a composition containing the same and applications thereof. The present ?-agarase provides the field a novel alternative and is favorable for the industrial utilities of the hydrolysis products of agarose. Furthermore, the present agarase is particularly modified for heterologous production by prokaryotic expression systems, and thereby is favorable for commercial use.
    Type: Grant
    Filed: March 20, 2017
    Date of Patent: November 3, 2020
    Assignee: AGRICULTURAL TECHNOLOGY RESEARCH INSTITUTE
    Inventors: Jiunn-Horng Lin, Jyh-Perng Wang, Zeng-Weng Chen, Hui-Jie Lin, Shu-Wei Chang, Weng-Zeng Huang, Jian-Fong Lai, Shih-Ling Hsuan
  • Patent number: 10792333
    Abstract: The present invention relates to novel peptides derived from Actin-like protein 8 (ACTL8), complexes comprising such peptides bound to recombinant MHC molecules, and cells presenting said peptide in complex with MHC molecules. Also provided by the present invention are binding moieties that bind to the peptides and/or complexes of the invention. Such moieties are useful for the development of immunotherapeutic reagents for the treatment of diseases such as cancer.
    Type: Grant
    Filed: November 23, 2016
    Date of Patent: October 6, 2020
    Assignee: IMMUNOCORE LIMITED
    Inventors: Alex Powlesland, Alina Popa, Philip Addis
  • Patent number: 10655118
    Abstract: The present invention relates to a heat-resistant agarase and a monosaccharide production method using same. More particularly, in the present invention, a heat-resistant agarase may be used to produce galactose and 3,6-anhydro-L-galactose at high yield by efficiently breaking down agarose or agar without a chemical pretreatment, a neutralization process, or an agarotriose hydrolase treatment process.
    Type: Grant
    Filed: January 17, 2017
    Date of Patent: May 19, 2020
    Assignee: Korea University Research and Business Foundation
    Inventors: Kyoung Heon Kim, In-Geol Choi, Jung Hyun Kim, Eun-Ju Yun
  • Patent number: 10407700
    Abstract: A surfactant-improved simultaneous saccharification and co-fermentation method for lignocelluloses in which a pretreated lignocellulose substrate is directly subjected to surfactant-improved simultaneous saccharification and co-fermentation without any detoxification by adding a surfactant, and the surfactant may be selectively added before or after pre-enzymatic hydrolysis, having economic, effective and feasible application prospects in reducing the loss of glucose, simplifying the production process, reducing equipment investment, reducing water consumption, improving ethanol production and reducing the total cost of the ethanol production process.
    Type: Grant
    Filed: December 29, 2014
    Date of Patent: September 10, 2019
    Assignee: DALIAN INSTITUTE OF CHEMICAL PHYSICS, CHINESE ACADEMY OF SCIENCES
    Inventors: Zongchao Zhang, Xiumei Liu, Liaoyuan Mao
  • Patent number: 10398716
    Abstract: Disclosed are methods of modulating and/or decreasing serum corticosterone levels in an individual affected by stress. Further disclosed are methods of modulating the hypothalamic pituitary adrenal response in an individual. The methods include administration of 2-fucosyl-lactose to an individual.
    Type: Grant
    Filed: September 12, 2013
    Date of Patent: September 3, 2019
    Assignee: ABBOTT LABORATORIES
    Inventors: Enrique Vazquez Hernandez, Ricardo Rueda Cabrera, Rachael Buck, Maria Ramirez Gonzalez
  • Patent number: 10398715
    Abstract: Disclosed are methods for enhancing learning and/or memory, enhancing memory acquisition, memory retention and recall by inducing a higher long-term potentiation in hippocampal neuronal synapsis in individuals. The methods include administration of 2-fucosyl-lactose to an individual.
    Type: Grant
    Filed: September 12, 2013
    Date of Patent: September 3, 2019
    Assignee: ABBOTT LABORATORIES
    Inventors: Enrique Vazquez Hernandez, Ricardo Rueda Cabrera, Rachael Buck, Maria Ramirez Gonzalez, Alejandro Barranco Perez
  • Patent number: 10334856
    Abstract: The present specification discloses pest control compositions, articles of manufacture, containers or kits comprising such compositions, and methods and uses to control a population of invertebrate pests from a mammal, location, plant, structure treated of such pest control compositions.
    Type: Grant
    Filed: August 22, 2016
    Date of Patent: July 2, 2019
    Assignee: Neozyme International, Inc.
    Inventors: Parker Dale, Parker David Dale
  • Patent number: 10272121
    Abstract: Disclosed herein is a topical composition that contains a fermentation extract from a simultaneous or sequential fermentation. When topically applied to skin, the composition of the invention is effective in stimulating the production of hyaluronic acid, CD44 and Caspase 14 protein in skin cells.
    Type: Grant
    Filed: October 25, 2012
    Date of Patent: April 30, 2019
    Assignee: ARCH PERSONAL CARE PRODUCT, LP
    Inventors: Smitha Rao, James Vincent Gruber
  • Patent number: 10266864
    Abstract: The present invention relates to thermostable pullulanases useful for industrial and scientific purposes. The present invention provides methods for producing the modified pullulanase, enzymatic compositions comprising the modified pullulanase, and methods for use of the enzymatic compositions.
    Type: Grant
    Filed: March 10, 2014
    Date of Patent: April 23, 2019
    Assignee: BASF Enzymes LLC
    Inventors: Adrienne Huston Davenport, Hugo D. Urbina, Kenneth E. Barrett, Danielle Cusumano
  • Patent number: 10131929
    Abstract: Enzymatic reactions are disclosed herein comprising water, glucose-1-phosphate, cellodextrin, and at least one cellodextrin phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6. These reactions produce a low molecular weight, insoluble cellulose with enhanced features.
    Type: Grant
    Filed: December 15, 2015
    Date of Patent: November 20, 2018
    Assignee: E. I. DU PONT DE NEMOURS AND COMPANY
    Inventors: Natnael Behabtu, Ayrookaran J. Poulose, Zheyong Yu, Zhenghong Zhang
  • Patent number: 9920110
    Abstract: In some embodiments, the invention relates to methods for creating a monoclonal antibody that specifically binds to antigen. The method may start from a polyclonal population of antibodies such as a non-specific polyclonal population or a polyclonal population of antibodies that specifically bind to the antigen. The method includes obtaining nucleic acid molecules encoding heavy and light immunoglobulin chains (or variable regions thereof) of multiple immunoglobulins from an animal; obtaining mass spectra information of peptide fragments of a population of polyclonal immunoglobulins that specifically bind to an antigen of choice; comparing and/or correlating the mass spectra information of the peptide fragments of the polyclonal immunoglobulins with predicted mass spectra information of predicted amino acid sequences encoded by the nucleic acid molecules, and then assembling the heavy and light chains to create an antibody (or variable region thereof) that specifically binds to the antigen.
    Type: Grant
    Filed: March 9, 2012
    Date of Patent: March 20, 2018
    Assignee: Cell Signaling Technology, Inc.
    Inventors: Roberto Polakiewicz, Wan Cheung Cheung, John Edward Rush, II, Sean Andre Beausoleil
  • Patent number: 9872914
    Abstract: This solid pharmaceutical composition is useful as a solid pharmaceutical composition of 1-(3-(2-(1-benzothiophen-5-yl)ethoxy)propyl)azetidin-3-ol, which is excellently elutable and moldable and is stable in long-term storage, or a salt thereof.
    Type: Grant
    Filed: February 21, 2013
    Date of Patent: January 23, 2018
    Assignee: TOYAMA CHEMICAL CO., LTD.
    Inventors: Hiroyuki Inaba, Mitsuhiro Nagata
  • Patent number: 9763463
    Abstract: Disclosed is a process to extract peptides and maltodextrins from quinoa flour for the manufacturing of foodstuff corresponding to a gel for sportspeople consumption during and after physical activity.
    Type: Grant
    Filed: June 23, 2014
    Date of Patent: September 19, 2017
    Inventors: Javier Ignacio Enrione Caceres, Paulo Diaz Calderon, Fernando Osorio Lira
  • Patent number: 9725702
    Abstract: The invention addresses the need for enzymes that can enhance the yield of fermentable sugar from the hydrolysis of lignocellulose biomass, for example sugar cane bagasse, barley straw and wheat straw, such that the use of this biomass can become economically viable. The invention provides methods for the hydrolysis of biomass using a laccase derived from Ganoderma lucidum. Further, the invention provides an enzyme composition comprising a laccase derived from Ganoderma lucidum which may be combined with one or more cellulases, and for its use in enhancing lignocellulose biomass hydrolysis.
    Type: Grant
    Filed: September 11, 2013
    Date of Patent: August 8, 2017
    Assignee: DANMARKS TEKNISKE UNIVERSITET
    Inventors: Anna Sitarz, Jørn Dalgaard Mikkelsen, Anne Meyer, Mateusz Lezyk
  • Patent number: 9279134
    Abstract: The present invention relates to compositions and methods to identify novel bacteria and metabolites derived therefrom. More specifically, the invention describes a novel method to isolate bacteria producing metabolites of interest from environmental samples. Particularly, the invention discloses a method to select rare antibiotic producing bacteria. The invention can be used from any sample and allows the isolation of bacteria having e.g., pharmaceutical or agrochemical interest.
    Type: Grant
    Filed: September 18, 2013
    Date of Patent: March 8, 2016
    Assignees: DEINOVE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
    Inventors: Jean-Paul Leonetti, Stéphanie Texier
  • Patent number: 9200303
    Abstract: The present invention describes a process for preparing ultrapure (?95%) galacto-oligosaccharides (GOS), starting from GOS at lower purities by using sequential microbiological purifications involving Saccharomyces cerevisiae and Streptococcus thermophilics.
    Type: Grant
    Filed: August 6, 2010
    Date of Patent: December 1, 2015
    Assignee: Ritter Pharmaceuticals, Inc.
    Inventors: Silvia Giacomelli, Marco Manoni, Giovanni Cipolletti, Silvia Biagiolini, Luana Vagnoli, Jacopo Chini
  • Publication number: 20150126472
    Abstract: The present invention relates to a strain of Serratia plymuthica capable of producing turanose and to the uses thereof.
    Type: Application
    Filed: May 15, 2013
    Publication date: May 7, 2015
    Applicant: ROQUETTE FRERES
    Inventors: Sophie Defretin, Damien Raeckelboom
  • Patent number: 9005946
    Abstract: The invention provides variants of the Clostridium thermocellum endoglucanase (CelG) that have improved endoglucanase activity compared to the wild type enzyme. Also provided are related polynucleotides, compositions, vectors, host cells, and methods of use.
    Type: Grant
    Filed: March 10, 2010
    Date of Patent: April 14, 2015
    Assignee: Codexis, Inc.
    Inventors: Jie Yang, Xiyun Zhang, Ish Kumar Dhawan, Andrew Shaw
  • Patent number: 8993275
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: March 31, 2015
    Assignee: Novozymes, Inc.
    Inventor: Nikolaj Spodsberg
  • Patent number: 8975058
    Abstract: The present invention relates to production of fermentable sugars from lignocellulosic material by enzymatic conversion. The fermentable sugars are useful e.g. in the production of bioethanol. Novel polypeptides having endoglucanase activity, polynucleotides encoding them and vectors and host cells containing the polynucleotides are disclosed. A method for treating cellulosic material with the novel endoglucanase as well as uses of the enzymes and enzyme preparations and a method of preparing them are described.
    Type: Grant
    Filed: May 24, 2013
    Date of Patent: March 10, 2015
    Assignee: Roal Oy
    Inventors: Taija Leinonen, Alexandra Komander, Kim Langfelder, Jari Vehmaanperä, Terhi Puranen
  • Publication number: 20150018522
    Abstract: Disclosed are mutants of galactosyltransferases that can catalyze formation of oligosaccharides in the presence of magnesium; mutants of galactosyltransferases having altered donor and acceptor specificity which can catalyze formation of oligosaccharides in the presence of magnesium; methods and compositions that can be used to synthesize oligosaccharides; methods for increasing the immunogenicity of an antigen; and methods to stabilize platelets.
    Type: Application
    Filed: March 10, 2014
    Publication date: January 15, 2015
    Applicant: The United States of America, as represented by the Secretary, Department of Health & Human Servic
    Inventors: Pradman K. Qasba, Elizabeth Boeggeman, Boopathy Ramakrishnan
  • Publication number: 20140349348
    Abstract: The present invention relates to mutated and/or transformed microorganisms for the synthesis of various compounds. More specifically, the present invention discloses microorganisms mutated in the genes encoding for the regulators ArcA and IclR. The latter mutations result in a significant upregulation of the genes that are part of the colanic acid operon. Hence, said microorganisms are useful for the synthesis of any compound being part of the colanic acid pathway such as GDP-fucose, GDP-mannose and colanic acid, and/or, can be further used—starting form GDP-fucose as a precursor—to synthesize fucosylated oligosaccharides or—starting from GDP-mannose as a precursor—to synthesize mannosylated oligosaccharides. In addition, mutations in the genes coding for the transcriptional regulators ArcA and IclR lead to an acid resistance phenotype in the exponential growth phase allowing the synthesis of pH sensitive molecules or organic acids.
    Type: Application
    Filed: December 14, 2012
    Publication date: November 27, 2014
    Inventors: Joeri Beauprez, Gaspard Lequeux, Jo Maertens
  • Publication number: 20140287458
    Abstract: The present invention provides a method of producing a photosynthetic product, the method comprising maintaining a photosynthetic plant or algal cell suspension culture, in the presence of water, light and a carbonic acid-enriched growth medium. The carbonic acid may, for example be provided by feeding the photosynthetic plant cell suspension culture with a carbonic acid solution, a solid or liquid precursor thereof, or a gaseous mixture of carbon dioxide and one or more other gases. The invention also provides a method for producing a photosynthetic product, the method comprising maintaining a photosynthetic plant or algal cell suspension culture, in the presence of water, light and a carbon source selected from carbon dioxide and carbonic acid, wherein the culture is maintained at a pH of less than 7.0, preferably 4.5 to 5.5.
    Type: Application
    Filed: May 18, 2012
    Publication date: September 25, 2014
    Applicant: NATURALLY SCIENTIFIC TECHNOLOGIES LIMITED
    Inventor: Peter Andrew Whitton
  • Publication number: 20140287471
    Abstract: The present disclosure relates to variant CBH I polypeptides that have reduced product inhibition, and compositions, e.g., cellulase compositions, comprising variant CBH I polypeptides. The variant CBH I polypeptides and related compositions can be used in variety of agricultural and industrial applications. The present disclosure further relates to nucleic acids encoding variant CBH I polypeptides and host cells that recombinantly express the variant CBH I polypeptides.
    Type: Application
    Filed: October 5, 2012
    Publication date: September 25, 2014
    Applicant: Bp Corporation North America Inc.
    Inventors: Sarah Richardson Hanson, Justin T. Stege, Cecilia Cheng, Peter Luginbuhl
  • Publication number: 20140234912
    Abstract: A method for generating human milk oligosaccharides (HMOs) or precursors thereof, compounds obtainable by the method, and uses and compositions involving such compounds. The method comprising the steps of a) providing at least one donor selected from the group of compounds of any of formulae 5 to 10, b) providing at least one acceptor from a group of lactose, LNT, LNnT and derivatives thereof, c) providing at least one enzyme comprising a transglycosidase activity and/or a glycosynthase activity, d) preparing a mixture of the at least one donor, at least one acceptor and at least one enzyme provided in steps a), b) and c); and e) incubating the mixture prepared according to step d).
    Type: Application
    Filed: May 14, 2012
    Publication date: August 21, 2014
    Applicant: GLYCOM A/S
    Inventors: Gyula Dekany, Elise Champion, Andreas Schroven, Markus Hederos
  • Publication number: 20140227229
    Abstract: The present invention relates to gram positive bacteria with increased stress resistance and/or improved storage characteristics. In particular, the invention relates to gram positive bacterium which accumulate intracellular trehalose. The gram positive bacterium according to the invention lack cellobiose-specific PTS system IIC component (PtcC) activity. The gram positive bacterium may further lack trehalose 6-phosphate phosphorylase (TrePP) activity. The gram positive bacterium may further overexpress trehalose transporters. The invention further relates to compositions comprising such gram positive bacterium as well as methods and uses thereof.
    Type: Application
    Filed: September 21, 2012
    Publication date: August 14, 2014
    Inventors: Lothar Steidler, Karolien Van Huynegem, Klaas Vandenbroucke
  • Patent number: 8802398
    Abstract: A method of oxidizing a saccharide, by contacting the saccharide with an alcohol dehydrogenase (ADH) enzyme selected from a quinone redox cofactor-dependent ADH, a nicotinamide adenine dinucleotide (NAD+) redox cofactor-dependent ADH, a nicotinamide adenine dinucleotide phosphate (NADP+) redox cofactor-dependent ADH, and any combination thereof is described. An oxidized saccharide obtainable by the method and products, in particular food products and paper products, containing the oxidized saccharide, are also described.
    Type: Grant
    Filed: October 29, 2010
    Date of Patent: August 12, 2014
    Assignee: Dupont Nutrition Biosciences Aps
    Inventors: Jorn Dalgaard Mikkelsen, Karsten Matthias Kragh, Rene Mikkelsen, Patrick Maria Franciscus Derkx, Shukun Yu, Harm Mulder, Igor Nikolaev
  • Patent number: 8795989
    Abstract: The present invention is directed to methods and systems of producing neoagarobiose, useful in whitening melanoma cells and in cosmetics, using polypeptides having neoagarobiosebiohydralase activity, including Aga86E from Saccharophagus degradans. The reaction can be enhanced by including other agarases, including Aga16B, also from S. degradans.
    Type: Grant
    Filed: April 30, 2008
    Date of Patent: August 5, 2014
    Assignee: University of Maryland
    Inventors: Steven W. Hutcheson, Ronald M. Weiner, Nathan A. Ekborg
  • Patent number: 8778640
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: July 15, 2014
    Assignee: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Patent number: 8778641
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: July 15, 2014
    Assignee: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Patent number: 8778639
    Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: July 15, 2014
    Assignee: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Patent number: 8771993
    Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: July 8, 2014
    Assignees: Novozymes A/S, Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Patent number: 8771994
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: July 8, 2014
    Assignee: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Patent number: 8759039
    Abstract: The present invention relates to the isolation and to the characterization of two proteins having a novel enzymatic activity, i.e. a porphyranase activity. These proteins are useful for hydrolyzing polysaccharides containing sulfated agaro-colloids and for producing oligo-porphyrans, notably oligo-porphyrans with a defined structure and size.
    Type: Grant
    Filed: July 20, 2010
    Date of Patent: June 24, 2014
    Assignee: Centre National de la Recherche Scientifique (C.N.R.S.)
    Inventors: Jan-Hendrik Hehemann, Gaëlle Correc, Gurvan Michel, Tristan Barbeyron, William Helbert, Mirjam Czjzek
  • Patent number: 8759040
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: June 24, 2014
    Assignee: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Patent number: 8759041
    Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: June 24, 2014
    Assignee: Novozymes Inc.
    Inventor: Nikolaj Spodsberg
  • Patent number: 8759033
    Abstract: The present invention relates to genetically modified actinobacteria for the production of an enzyme having chitosanase activity. The genetically modified actinobacteria have a reduced (or abolished) activity of the CsnR polypeptide. Such reduced activity can be obtained by reducing the capacity of expressing the csnR gene, its corresponding transcript or expressing a dominant-negative CsnR polypeptide. Such genetically modified actinobacteria are less dependent (and, in some embodiment, totally independent) on the presence of chitosan in the culture medium for producing an enzyme having chitosanase activity. In addition, the genetically modified bacteria produce less proteases in the culture medium and ultimately provide a chitosanase end-product with higher purity.
    Type: Grant
    Filed: February 8, 2013
    Date of Patent: June 24, 2014
    Assignee: Socpra Sciences et Genie S.E.C.
    Inventors: Ryszard Brzezinski, Marie-Pierre Dubeau
  • Patent number: 8753860
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: June 17, 2014
    Assignee: Novozymes A/S
    Inventors: Marc D. Morant, Paul Harris
  • Publication number: 20140154751
    Abstract: The present disclosure provides compositions and methods for the fermentation of cellodextrins and ?-D-glucose. Host cells and recombinant polypeptides having glucose mutarotase activity are provided. Additionally, methods for improving cell growth, production of chemicals, and consumption of cellodextrins and ?-D-glucose during fermentation of mixtures containing cellodextrins and ?-D-glucose are provided.
    Type: Application
    Filed: January 20, 2012
    Publication date: June 5, 2014
    Applicants: The Regents of the Univeristy of California, The Board of Trustee of the University of Illinois
    Inventors: James H. Doudna Cate, William T. Beeson, IV, Jonathan M. Galazka, Huimin Zhao, Sijin Li, Yong-Su Jin, Suk-Jin Ha
  • Patent number: 8728770
    Abstract: A method for the enzymatic saccharification of a lignocellulosic raw material, including adding a pretreated lignocellulosic raw material as a material suitable for an enzymatic saccharification reaction, together with an electrolyte containing a water-soluble salt, to water that contains a celluolose saccharification enzyme; saccharifying the raw material by an enzymatic saccharification reaction, as a suspension of the raw material having an electrical conductivity adjusted to 5-25 mS/cm; separating and recovering a reaction product and an enzyme-containing solution from the enzymatically saccharified treatment suspension; and recycling the recovered enzyme-containing solution as the enzyme for the enzymatic saccharification step.
    Type: Grant
    Filed: August 31, 2011
    Date of Patent: May 20, 2014
    Assignee: Oji Holdings Corporation
    Inventors: Kotaro Ishikawa, Atsushi Furujyo, Yaping Chao, Hisako Tokuno, Jun Sugiura, Motohiro Matsumura
  • Patent number: 8715969
    Abstract: A method for the production of a saccharification product is provided, the method comprising: a) providing a cellulosic feedstock comprising lignin; b) contacting, in water, the feedstock of (a) with at least one metal carbonate at time 0 hour under suitable initial reaction conditions to produce a first delignification slurry; c) contacting the first delignification slurry with a nucleophilic base at time (0+n) hour, where n represents a number greater than zero, under suitable secondary reaction conditions to form a second delignification slurry comprising a lignin-containing liquid fraction and a polysaccharide-enriched solid fraction; d) separating the lignin-containing liquid fraction from the polysaccharide-enriched solid fraction; and e) contacting at least a portion of the polysaccharide-enriched solid fraction with an enzyme consortium to produce a saccharification product comprising xylose and glucose monomers wherein the overall yield of xylose and glucose monomers in the saccharification produc
    Type: Grant
    Filed: November 19, 2009
    Date of Patent: May 6, 2014
    Assignee: E I du Pont de Nemours and Company
    Inventors: Subramaniam Sabesan, Christina Jacy Spado
  • Patent number: 8647845
    Abstract: Provided is a method for efficiently producing sugar and simultaneously efficiently producing ethanol. A method for producing sugar characterized by comprising a pretreatment step in which a plant-origin sugar solution is fermented by a microorganism having no sucrose-degrading enzyme and a step for producing sugar from the fermented sugar solution. A method for producing sugar characterized by comprising a pretreatment step in which a plant-origin sugar solution is fermented by a microorganism in the presence of a sucrose-degrading enzyme inhibitor and a step for producing sugar from the fermented sugar solution.
    Type: Grant
    Filed: March 5, 2013
    Date of Patent: February 11, 2014
    Assignees: Asahi Group Holdings, Ltd., Incorporated Administrative Agency National Agriculture and Food Research Organization
    Inventors: Satoshi Ohara, Yoshifumi Terajima, Akira Sugimoto
  • Publication number: 20140004574
    Abstract: The processes disclosed herein include saccharifying cellulosic and/or lignocellulosic biomass and fermenting the sugars to produce a sugar alcohol.
    Type: Application
    Filed: September 3, 2013
    Publication date: January 2, 2014
    Applicant: XYLECO, INC.
    Inventors: Marshall MEDOFF, Thomas Craig MASTERMAN, Jaewoong MOON, Aiichiro YOSHIDA
  • Publication number: 20130340119
    Abstract: A method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a POI (Protein Of Interest) polypeptide is provided. Methods for the production of plants having modulated expression of a nucleic acid encoding a DnaJ-like chaperone polypeptide are provided, in which plants have enhanced yield-related traits compared to control plants. Nucleic acids encoding DnaJ-like chaperone, constructs comprising the same and uses thereof are also provided.
    Type: Application
    Filed: October 27, 2011
    Publication date: December 19, 2013
    Applicant: BASF Plant Science Company GmbH
    Inventors: Gunnar Plesch, Astrid Blau, Michael Manfred Herold, Beate Kamlage, Birgit Wendel, Piotr Puzio, Oliver Bläsing, Oliver Thimm, Janneke Hendriks, Christophe Reuzeau
  • Patent number: 8597914
    Abstract: Provided herein is a transgenic bacteria engineered to accumulate carbohydrates, for example disaccharides. Also provided is a photobioreactor for cultivating photosynthetic microorganisms comprising a non-gelatinous, solid cultivation support suitable for providing nutrients and moisture to photosynthetic microorganisms and a physical barrier covering at least a portion of the surface of the cultivation support. Devices for the large scale and continuous cultivation of photosynthetic microorganisms incorporating photobioreactors and methods of use are disclosed. Also disclosed are methods of producing fermentable sugar from photosynthetic microorganisms using a photobioreactor of the invention.
    Type: Grant
    Filed: January 9, 2013
    Date of Patent: December 3, 2013
    Assignee: Proterro, Inc.
    Inventors: John Aikens, Robert J. Turner
  • Patent number: 8580552
    Abstract: The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used, for example for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology are described, as well as uses of the enzymes and enzyme preparations.
    Type: Grant
    Filed: December 30, 2010
    Date of Patent: November 12, 2013
    Assignee: Royal Oy
    Inventors: Terhi Puranen, Sauli Toikka, Kim Langfelder, Jari Vehmaanpera
  • Patent number: 8563277
    Abstract: Provided are methods and compositions for high yields while using reduced enzyme loads in saccharification and fermentation processes. These methods increase the efficiency of enzymes and result in improved yields and composition of saccharification and fermentation end products.
    Type: Grant
    Filed: November 27, 2012
    Date of Patent: October 22, 2013
    Assignee: Sweetwater Energy, Inc.
    Inventors: Sarad Parekh, Carl P. Felice
  • Patent number: 8563276
    Abstract: The invention relates to a method for enzyme cleavage of polysaccharides comprising a first sequence [?4)-?-D-GlcpA-(1?4)-?-L-Rhap3 sulphate-(1?]n and a second sequence [?4)-?-L-IdopA-(1?4)-?-L-Rhap3 sulphate-(1?]m, the first and second sequences respectively comprising two monosaccharide units connected by an osidic bond, wherein said method is such that: said polysaccharide sequences are provided; a microorganism capable of producing an enzyme substance of the lyase class is provided; and said enzyme substance is brought into contact with said polysaccharide sequences in such a way as to bring about cleavage of the osidic bond according to a ?-elimination reaction. The invention is characterized in that a microorganism belonging to the bacteria of the Ochrobactrum genus is chosen for producing said enzyme substance.
    Type: Grant
    Filed: June 23, 2008
    Date of Patent: October 22, 2013
    Assignee: Universite de Picardie Jules Verne
    Inventors: Redouan El Boutachfaiti, Patrice Pheulpin, Bernard Courtois, Josiane Courtois-Sambourg