Monosaccharide Patents (Class 435/105)
  • Patent number: 11307146
    Abstract: Methods, devices and kit for analyzing a sample comprising 1,5-anhydroglucitol and a first analyte via one or more chemiluminescent reactions. Certain embodiments include measuring a first light response resulting from a first chemiluminescent reaction and measuring a second light response resulting from a second chemiluminescent reaction. Certain embodiments also include comparing the first light response to the second light response to determine a ratio of 1,5-anhydroglucitol and the first analyte.
    Type: Grant
    Filed: July 27, 2017
    Date of Patent: April 19, 2022
    Assignees: University of Houston System, Instituto Tecnológico y de Estudios Superiores de Monterrey
    Inventors: Richard C. Willson, Jinsu Kim, Binh V. Vu, Olga Patricia Vázquez Villegas, Federico Augusto Ruiz Ruiz, Marco Antonio Rito Palomares
  • Patent number: 11180723
    Abstract: The invention relates to a method for the simultaneous production of a solid transformation product of the substrate and crude ethanol comprising the following steps: •preparing a substrate from milled or flaked biomass comprising proteinaceous matter which originates from soya bean, rape seed, or mixtures thereof, optionally in further mixture with proteinaceous matter originating from fava beans, peas, sunflower seeds, lupine, cereals, and/or grasses, •mixing said substrate with live yeast in a dry matter ratio of from 1:1 to 10,000:1 and adding water in an amount which provides a ratio of wet bulk density to dry bulk density from 0.60 to 1.45 in the resulting mixture; •incubating said mixture for 1-48 hours at a temperature of about 20-60° C.
    Type: Grant
    Filed: June 27, 2017
    Date of Patent: November 23, 2021
    Assignee: HAMLET PROTEIN A/S
    Inventors: Katrine Hvid Ellegård, Jonatan Ahrens Dickow, Stig Victor Petersen, Laila Thirup, Svend Andreas Geleff
  • Patent number: 11028421
    Abstract: The disclosure discloses recombinant Pseudomonas plecoglossicida for producing L-xylose and application thereof, and belongs to the technical field of bioengineering. According to the disclosure, a synthesized 2-ketogluconate reductase gene and a 2,5-diketogluconate reductase gene derived from Corynebaterium ATCC 31090 and a pyruvate decarboxylase gene derived from Saccharomyces cerevisiae are successfully expressed in a host P. plecoglossicida by a double plasmid system, and an obtained genetically engineered strain is fermented for 56 h in a shake flask, where the yield of L-xylose reaches 16.2 g/L, and the transformation rate reaches 20.3%; the obtained genetically engineered strain is fermented for 48 h and 44 h in 3 L and 15 L fermentors, respectively, where the yields of L-xylose reach 37.6 g/L and 45.8 g/L, respectively, and the glucose transformation rates are 47.0% and 57.3%, respectively.
    Type: Grant
    Filed: November 28, 2019
    Date of Patent: June 8, 2021
    Assignee: LINKCHEM CO. LTD.
    Inventor: Xi Lu
  • Patent number: 11008598
    Abstract: Provided herein is a process for producing a fermentation product from a lignocellulosic feedstock. The process comprises soaking a lignocellulosic feedstock in an aqueous solution to produce a soaked feedstock. The soaked feedstock is at least partially dewatered and the at least partially dewatered feedstock is subjected to pretreating. The pretreatment chemical is sulfur dioxide, sulfurous acid or a combination thereof and can be added to the process at any stage prior to and/or during pretreatment. The pretreated feedstock composition is fed to an enzymatic hydrolysis conducted at a temperature that is higher than 58° C. The cellulose in the pretreated feedstock composition is hydrolyzed with cellulase enzymes in the presence of the dissolved solids to produce glucose. The glucose is fermented to produce the fermentation product.
    Type: Grant
    Filed: March 16, 2016
    Date of Patent: May 18, 2021
    Assignee: Iogen Corporation
    Inventors: Brian Foody, Jeffrey S. Tolan
  • Patent number: 10995314
    Abstract: A process for producing ethanol from lignocellulosic biomass includes adding at least one of sulfur dioxide and sulfurous acid to the lignocellulosic biomass to provide an equivalent sulfur dioxide loading of at least 10 wt % sulfur dioxide to dry lignocellulosic biomass. The acidified lignocellulosic biomass is pretreated at a temperature above about 185° C. and for a pretreatment time less than about 10 minutes, to provide a pretreated biomass composition wherein the biomass is readily hydrolyzed by enzymes. Advantageously, sulfur dioxide from at least one of the flash stream and a stream derived from the flash is recovered and recycled back into the process.
    Type: Grant
    Filed: March 16, 2016
    Date of Patent: May 4, 2021
    Assignee: Iogen Corporation
    Inventors: Brian Foody, Daniel G. MacDonald, Kristin Martens, Natacha Leduc, John Dechman, Robert Griffin
  • Patent number: 10960401
    Abstract: A process for the decomposition of biomass-material includes subjecting a lignocellulose-containing biomass-material to comminution, subjecting the comminuted lignocellulose-containing biomass-material to a sifting to separate from the comminuted lignocellulose-containing biomass-material a fraction of small-particles, and subjecting the remaining comminuted lignocellulose-containing biomass-material to a pretreatment. Before, during, or after the pretreatment, small particles can be added to the remaining comminuted lignocellulose-containing biomass-material. Optionally, the small particles can be added continuously during the pretreatment. The addition of small particles decreases friction of the remaining comminuted lignocellulose-containing biomass-material results in decreased process time, energy savings, and reduced production costs.
    Type: Grant
    Filed: July 10, 2017
    Date of Patent: March 30, 2021
    Assignee: Clariant International Ltd.
    Inventors: Ralf Hortsch, Thomas Hoppe, Bjoern Huehnlein
  • Patent number: 10947566
    Abstract: A hydrogen peroxide production method, system, and apparatus is provided for producing large volumes of hydrogen peroxide having concentrations up to and excess of 80% in one continuous cycle. In one aspect, the hydrogen peroxide production system can include an aqueous solution comprised of an NQO1 enzyme, an NQO1 activated compound or molecule, and an NADH or NADPH cofactor for producing hydrogen peroxide, a production chamber having a semi-permeable membrane for receiving the aqueous solution. Here, the membrane can further include one or more molecular weight barriers configured to diffuse the produced hydrogen peroxide there through. The system can also include a collection chamber for receiving the produced hydrogen peroxide, and one or more pumps for circulating the aqueous solution having the hydrogen peroxide to the collection chamber and back to the production chamber.
    Type: Grant
    Filed: January 5, 2018
    Date of Patent: March 16, 2021
    Inventor: Gaurab Chakrabarti
  • Patent number: 10889795
    Abstract: Discharging pretreated biomass from a pretreatment reactor and mixing the discharged pretreated biomass with a cooling liquid in a vessel provides a cooled slurry having a consistency that is less than about 12 wt %. Since the consistency is relatively low, the cooled slurry may be pumped to a higher elevation using standard pumping equipment. At the higher elevation, the cooled slurry may be separated into a first stream comprising a liquid component of the slurry and a second other stream comprising a solid component of the slurry (e.g., having a consistency between about 15 wt % and 40 about wt %). The solid component may be fed to an inlet of a hydrolysis reactor, while the liquid component may be fed to a cooling system that provides a cooled stream. The cooled stream may then be cycled back to the vessel.
    Type: Grant
    Filed: November 24, 2016
    Date of Patent: January 12, 2021
    Assignee: IOGEN ENERGY CORPORATION
    Inventors: Stephen A. Rowland, Chris Mallalieu, Ryan Taylor, Ziyad Rahme
  • Patent number: 10876122
    Abstract: Provided is a method of producing 2?-fucosyllactose including culturing in a medium supplemented with lactose a recombinant Corynebacterium glutamicum transformed to express ?-1,2-fucosyltransferase, transformed to express GDP-D-mannose-4,6-dehydratase, transformed to express GDP-L-fucose synthase, and transformed to express lactose permease, wherein the recombinant Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase.
    Type: Grant
    Filed: October 1, 2019
    Date of Patent: December 29, 2020
    Assignees: SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION, ADVANCED PROTEIN TECHNOLOGIES CORP.
    Inventors: Jin-Ho Seo, Young-Wook Chin, Hae-Yong Jo
  • Patent number: 10814018
    Abstract: The present invention provides isotopically labeled deoxy-glucose and derivatives thereof, methods of their preparation, ration, kits comprising them and uses thereof for spin hyperpolarized magnetic resonance imaging, utilized in the quantitative and qualitative diagnosis of states, conditions, diseases, or disorders in the body of a subject.
    Type: Grant
    Filed: October 25, 2011
    Date of Patent: October 27, 2020
    Assignee: HADASIT MEDICAL RESEARCH SERVICE & DEVELOPMENT LTD.
    Inventors: Moshe Gomori, Hyla Allouche-Arnon, Rachel Katz-Brull
  • Patent number: 10730750
    Abstract: Provided is an unique, efficient and cost-effective process for the recovery of acid from acid-rich solutions. The process of the subject matter utilizes a strong oxidizer, such as Caro's acid, to disintegrate or render insoluble organic or inorganic materials such as carbohydrates and complexes thereof contained in acid-rich solutions, to make efficient and simple the separation and recovery of the acid solution. The acid recovered thus obtained is free of organic matter, and containing nearly all of the acid originally contained in the acid-rich solution.
    Type: Grant
    Filed: April 2, 2019
    Date of Patent: August 4, 2020
    Assignee: MELODEA LTD.
    Inventors: Shaul Lapidot, Clarite Azerraf, Inbar Kremer
  • Patent number: 10704069
    Abstract: The current disclosure provides a process for enzymatically converting a saccharide into allulose. The invention also relates to a process for preparing allulose where the process involves converting fructose 6-phosphate (F6P) to allulose 6-phosphate (A6P), catalyzed by allulose 6-phosphate 3-epimerase (A6PE), and converting the A6P to allulose, catalyzed by allulose 6-phosphate phosphatase (A6PP).
    Type: Grant
    Filed: April 17, 2019
    Date of Patent: July 7, 2020
    Assignee: BONUMOSE LLC
    Inventors: Daniel Joseph Wichelecki, Edwin O. Rogers
  • Patent number: 10696993
    Abstract: Improved enzyme based methods of separating protein from protein-rich material are provided. A method can include utilizing a modeling equation to more effectively hydrolyze the various types of carbohydrates present in a protein-rich material. A method can include a fed-batch method of incrementally adding a protein-rich material, an enzyme broth, or both a protein-rich material and an enzyme broth. A method can also include partially or completely recycling the hydrolysate.
    Type: Grant
    Filed: April 20, 2016
    Date of Patent: June 30, 2020
    Assignee: The University of Akron
    Inventors: Lu-Kwang Ju, S M Mahfuzul Islam, Qian Li, Abdullah Al Loman
  • Patent number: 10689668
    Abstract: The present invention relates to a recombinant strain for producing an allose from a fructose, a composition for producing an allose which produces an allose from a fructose-containing raw material comprising the strain, and a method for preparing an allose using the same.
    Type: Grant
    Filed: December 1, 2016
    Date of Patent: June 23, 2020
    Assignee: SAMYANG CORPORATION
    Inventors: Min Jeong Kim, Eun Jin Han, Jeong Min Kim, Hye Jung Kim, Chong Jin Park, Kang Pyo Lee, Eun-Soo Choi, Jeongyoon Choi
  • Patent number: 10676764
    Abstract: The present invention relates to a novel and inventive process for the production of sorbitol from D-sucrose.
    Type: Grant
    Filed: July 14, 2016
    Date of Patent: June 9, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Mae Joanne Aguila, Hans-Peter Hohmann, Laurent Lefort, Jonathan Alan Medlock, Guenter Pappenberger
  • Patent number: 10595551
    Abstract: Processes for the preparation of an insoluble biomass gel involving: (a) mixing ground agricultural materials and hexane to produce de-oiled agricultural materials, (b) treating the de-oiled agricultural materials with thermostable ?-amylase to produce de-oiled and de-starched agricultural materials, (c) mixing alkali with the de-oiled and de-starched agricultural materials to produce a first residue, (d) mixing hydrogen peroxide with the first residue to form a second residue, and (e) mixing the second residue in water and collecting an insoluble material which is an insoluble biomass gel.
    Type: Grant
    Filed: June 22, 2015
    Date of Patent: March 24, 2020
    Assignees: The United States of America, as represented by the Secretary of Agriculture, AgriFiber Holdings LLC
    Inventors: Madhav P. Yadav, Kevin B. Hicks, David Johnston, Kyle A. Hanah, Madhuvanti S. Kale
  • Patent number: 10570399
    Abstract: Disclosed are a recombinant Corynebacterium glutamicum (C. glutamicum) for producing fucosyllactose which is transformed to express ?-1,2-fucosyltransferase, GDP-D-mannose-4,6-dehydratase (Gmd), GDP-L-fucose synthase (WcaG) and lactose permease (LacY), wherein the Corynebacterium glutamicum has phosphomannomutase and GTP-mannose-1-phosphate guanylyltransferase, and a method for producing fucosyllactose using the same. According to the recombinant Corynebacterium glutamicum and the method for producing fucosyllactose according to the present invention, with use of a GRAS Corynebacterium glutamicum strain, which is safer than conventional Escherichia coli, 2?-fucosyllactose can be produced at a high concentration while overcoming drawbacks of conventional methods associated with industrial inapplicability resulting from low production concentrations.
    Type: Grant
    Filed: April 24, 2017
    Date of Patent: February 25, 2020
    Assignee: Seoul National University R&DB Foundation
    Inventors: Jin-Ho Seo, Young-Wook Chin, Hae-Yong Jo
  • Patent number: 10533194
    Abstract: A system and method for converting biomass with no chemical pretreatment is disclosed. Combination of a microbial system and the use of mechanical disruption during fermentation may help achieve high conversion rate without the extra cost and undesirable by-products typically associated with the pretreatment process.
    Type: Grant
    Filed: December 14, 2015
    Date of Patent: January 14, 2020
    Assignee: THE TRUSTEES OF DARTMOUTH COLLEGE
    Inventors: Lee R. Lynd, Julie M. D. Paye, Michael Balch
  • Patent number: 10501765
    Abstract: This disclosure provides a high solids biomass slurry that is readily pumpable and transportable to downstream processing units, such as chemical and/or biochemical processing units. The slurry is amenable to saccharification efficiencies of >70 % in processing times of <36 hours. Also provided are devices for processing materials, such as the high solids biomass slurry.
    Type: Grant
    Filed: March 25, 2016
    Date of Patent: December 10, 2019
    Assignee: Edeniq, Inc.
    Inventors: Jeffrey R. Charron, Thomas P. Griffin
  • Patent number: 10450698
    Abstract: The method for producing a cellulose-containing solid material of the invention includes treating a botanical biomass in a mixed solvent of water and at least one alcohol selected from aliphatic alcohols having 4 to 8 carbon atoms, under condition A (the concentration of the raw material to be charged into the mixed solvent is 1% by mass or more and 50% by mass or less), condition B (the reaction temperature is 100° C. or higher and 350° C. or lower), and condition C (the reaction time is 0.1 hours or more and 10 hours or less), followed by solid-liquid separation after the treatment to give a cellulose-containing solid material. Accordingly, there are provided a method for producing a cellulose-containing solid material excellent in saccharification performance and a method for producing glucose from the cellulose-containing solid material.
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: October 22, 2019
    Assignees: IDEMITSU KOSAN CO., LTD., NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY
    Inventors: Yoshihito Koyama, Takao Masuda, Teruoki Tago
  • Patent number: 10421768
    Abstract: The present invention is related to a biotransformation process, effected by means of an isolated polypeptide possessing benzopyrone phosphate synthetase activity, and also a microorganism comprising a nucleic acid sequence that encodes the polypeptide, for the preparation of phosphate-conjugated derivatives of benzopyrone compounds. The hydrophilic property of the benzopyrone compounds is enhanced after catalyzed by the benzopyrone phosphate synthetase of the present invention.
    Type: Grant
    Filed: April 25, 2017
    Date of Patent: September 24, 2019
    Assignee: NATIONAL TAIWAN UNIVERSITY
    Inventors: Nan-Wei Su, Chen Hsu, Shang-Ta Wang
  • Patent number: 10337041
    Abstract: The present invention relates to a method of making glucose syrup from liquefied starch comprising, (a) contacting the liquefied starch with a glucoamylase, a pullulanase, and optionally an alpha-amylase wherein the ratio of pullulanase dose expressed as NPUN/gDS, to alpha-amylase dose expressed as FAU(A)/gDS is at least 60, particularly at least 75, particularly at least 100, more particularly at least 150, more particularly at least 200, more particularly at least 250, more particularly at least 300, more particularly at least 400, more particularly at least 500, more particularly at least 600, more particularly at least 800 or if no alpha-amylase is present the pullulanse is present in a dose of at least 0.5, particularly at least 0.75, particularly at least 1.0, particularly at least 1.5 NPUN/gDS, and (b) saccharifying the liquefied starch.
    Type: Grant
    Filed: February 6, 2015
    Date of Patent: July 2, 2019
    Assignee: Novozymes A/S
    Inventors: Shiro Fukuyama, Keiichi Ayabe
  • Patent number: 10316342
    Abstract: Provided herein, in some embodiments, are systems, methods, and compositions (e.g., cells and cell lysates) for enzymatically converting a polymeric glucose carbohydrate (e.g., starch) to sugar.
    Type: Grant
    Filed: July 12, 2018
    Date of Patent: June 11, 2019
    Assignee: GreenLight Biosciences, Inc.
    Inventors: Daniel MacEachran, Drew S. Cunningham, William Jeremy Blake, Matthew Eduardo Moura
  • Patent number: 10294461
    Abstract: An object of the present invention is to modify a wild-type enzyme that is less reactive in the presence of an organic solvent to provide altered carbonyl reductases having better reactivity in the presence of the organic solvent than the wild-type enzyme, and/or to provide transformants producing such reductases. The present inventors have found altered carbonyl reductases having better reactivity in the presence of an organic solvent than the wild-type enzyme, from among a mutant enzyme library prepared by randomly mutating the wild-type enzyme gene, thereby arriving at completion of the present invention.
    Type: Grant
    Filed: March 25, 2014
    Date of Patent: May 21, 2019
    Assignee: KANEKA CORPORATION
    Inventors: Misato Matsui, Noriyuki Ito, Shigeru Kawano, Yoshihiko Yasohara
  • Patent number: 10266862
    Abstract: A method for obtaining psicose from a mixture of fructose and psicose includes adding a mannitol dehydrogenase to a mixture of fructose and psicose to convert fructose into mannitol, and separating the mannitol from the psicose. Accordingly, the psicose can be more easily isolated from the mannitol with high efficiency by using the method.
    Type: Grant
    Filed: November 6, 2015
    Date of Patent: April 23, 2019
    Assignee: INDUSTRY-ACADEMIC COOPERATION FOUNDATION GYEONGSANG NATIONAL UNIVERSITY
    Inventors: Seon-Won Kim, Min-Jin Choi, Seong-Hee Jeong, Dae-Yun Lee
  • Patent number: 10240217
    Abstract: A method including: (a) selectively reacting a first sugar in a mixture which includes at least one second sugar to form a product mixture comprising a product of said first sugar; (b) separating said product of said first sugar from said product mixture; and (c) separating at least one of said at least one second sugar from said product mixture.
    Type: Grant
    Filed: September 20, 2013
    Date of Patent: March 26, 2019
    Assignee: Virdia, Inc.
    Inventors: Robert Jansen, Aharon Eyal
  • Patent number: 10227623
    Abstract: A process for mechanical destructuring of cellulosic biomass was developed that makes use of a short application of high compression, impact, and shearing forces. The biomass may be destructured using a specific energy input that is less than 40% of the total combustible energy of the biomass. The destructured biomass, with or without saccharification and/or in-feed glycosyl hydrolase enzymes, may be used in feed applications.
    Type: Grant
    Filed: November 18, 2014
    Date of Patent: March 12, 2019
    Assignee: E I DU PONT DE NEMOURS AND COMPANY
    Inventors: Wathudura Indika Namal De Silva, Paul Joseph Fagan, F Glenn Gallagher, Zheng-Zheng Huang, Guangliang Gary Liu, Aaron Perelman, Luis Fernando Romero Millan, Anton Shpilsky, Daniel A Slanac, Maria Walsh
  • Patent number: 10220349
    Abstract: The present invention provides for a method for separating starch from processing solutions containing starch containing plants or roots such as potatoes, sweet potatoes, wheat, corn, tapioca, yams, cassaya, sago, rice, pea, broad bean, horse bean, sorghum, konjac, rye, buckwheat and barley to provide commercially acceptable starch while reducing disposal of solid or liquid waste matter into landfills or water treatment facilities.
    Type: Grant
    Filed: August 12, 2015
    Date of Patent: March 5, 2019
    Assignee: SMARTFLOW TECHNOLOGIES, INC.
    Inventors: Jason Bell, Mark Vander Hoff, Todd Benson, Marc Pugh, Joseph Peters
  • Patent number: 10144920
    Abstract: The present invention relates to variants of a parent cellobiohydrolase II. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: March 1, 2017
    Date of Patent: December 4, 2018
    Assignee: Novozymes, Inc.
    Inventor: Mark Wogulis
  • Patent number: 10113192
    Abstract: A method for the enzymatic production of D-fructose from D-glucose in a one-pot synthesis, wherein D-glucose is oxidized enzymatically to D-glucosone and D-glucosone is reduced enzymatically to D-fructose and the use of the D-fructose produced in this way for the production of furan derivatives.
    Type: Grant
    Filed: February 5, 2014
    Date of Patent: October 30, 2018
    Assignee: ANNIKKI GMBH
    Inventors: Ortwin Ertl, Marta Sut, Martina Brandner
  • Patent number: 10100076
    Abstract: The disclosed invention is a composition for and a method of seating a Flaviviridae (including BVDV and HCV), Orthomyxoviridae (including Influenza A and B) or Paramyxoviridae (including RSV) infection, or conditions related to abnormal cellular proliferation, in a host, including animals, and especially humans, using a nucleoside of general formula (I)-(XXIII) or its pharmaceutically acceptable salt or prodrug. This invention also provides an effective process to quantify the viral load, and in particular BVDV, HCV or West Nile Virus load, in a host, using real-time polymerase chain reaction (“RT-PCR”). Additionally, the invention discloses probe molecules that can fluoresce proportionally to the amount of virus present in a sample.
    Type: Grant
    Filed: June 3, 2013
    Date of Patent: October 16, 2018
    Assignee: Gilead Pharmasset LLC
    Inventors: Lieven Stuyver, Kyoichi Watanabe
  • Patent number: 10053662
    Abstract: Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can convert feedstock materials to a sugar solution, which can then be fermented to produce ethanol. Biomass feedstock is dispersed in a liquid medium and then saccharified.
    Type: Grant
    Filed: August 15, 2016
    Date of Patent: August 21, 2018
    Assignee: XYLECO, INC.
    Inventors: Marshall Medoff, Thomas Craig Masterman
  • Patent number: 10006033
    Abstract: Provided are microorganisms that catalyze the synthesis of chemicals and biochemicals from a methanol, methane and/or formaldehyde. Also provided are methods of generating such organisms and methods of synthesizing chemicals and biochemicals using such organisms.
    Type: Grant
    Filed: September 14, 2015
    Date of Patent: June 26, 2018
    Assignee: The Regents of the University of California
    Inventors: James C. Liao, Igor Bogorad
  • Patent number: 10006059
    Abstract: The present invention utilizes yeast Candida tropicalis (NRRL 12968) for xylitol production, as an alternative and unexplored strain with high bioconversion rate and stability at higher initial xylose concentration. Different parameters are optimized for batch fermentation of xylose to xylitol such as initial xylose concentration, aeration (vvm), agitation (rpm), percent inoculum addition, and oxygen transfer rate. Maximum xylitol yield of 0.7 g/g of xylose is obtained with 3.33% inoculum, 250 g/l of initial xylose concentration, 0.2 vvm of aeration rate, and two stage agitation strategy comprising of 500 rpm for 0-24 hrs and 400 rpm for 24-72 hrs at not more than 72 hrs of fermentation time. The present invention coins a novel process mode of fermentation where the batch process is extended with continuous fermentation at optimum dilution rate of 0.02/hr with effective residence time of 52 hrs. Productivity of ‘batch followed by continuous’ process is 2.5 gm/lit/hr which is 1.
    Type: Grant
    Filed: August 28, 2013
    Date of Patent: June 26, 2018
    Assignee: PRIVI BIOTECHNOLOGIES PRIVATE LIMITED
    Inventors: Manish Petkar, Shweta Pawar, Arvind M. Lali
  • Patent number: 9976195
    Abstract: The present invention relates to an energy-efficient process for the treatment of plant biomass, particularly sugar cane, for the production of carbohydrates and ethanol, using physico-chemical and extraction techniques, as well as very simple milling configurations, thereby minimizing energy consumption during extraction of the cane juice. The biomass treated and obtained through this process, when subjected to a fermentation process for the production of ethanol, increases the yield of the process in comparison with that of traditional sugar cane. It can also be used for the production of enzymes, animal feedstuffs, and other useful products.
    Type: Grant
    Filed: November 30, 2010
    Date of Patent: May 22, 2018
    Assignee: CTC—Centro de Tecnologia Canavieira—S.A.
    Inventors: Jose Augusto Travassos Rios Tome, Dionisio Fabiano Pegoretti, Dionisio Morelli Filho, Oswaldo Godoy Neto, Jaime Fingerut, Celia Maria Araujo Galvao, Liliane Pires Andrade
  • Patent number: 9957493
    Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: February 4, 2016
    Date of Patent: May 1, 2018
    Assignees: Novozymes, Inc., Novozymes A/S
    Inventors: Elena Vlasenko, Brett McBrayer, Mary Stringer, Kirk Schnorr
  • Patent number: 9868968
    Abstract: A method for producing saccharides according to the present invention includes a step of preparing a slurry by adding an aqueous solution containing a sugar or a sugar solution to biomass so that the slurry has a biomass content of 10 to 30 w/v %, and a step of adding at least one of an enzyme that degrades cellulose and an enzyme that degrades hemicellulose to the slurry containing the biomass to degrade at least one of cellulose and hemicellulose contained in the biomass, thereby producing saccharides containing glucose as a main component.
    Type: Grant
    Filed: October 4, 2013
    Date of Patent: January 16, 2018
    Assignee: JGC CORPORATION
    Inventor: Daisuke Taneda
  • Patent number: 9752169
    Abstract: The process for the hydrolysis of ligno-cellulosic biomass comprises the steps of A) Contacting a ligno-cellulosic feedstock, the feedstock comprised of biomass having a dry content and water with at least a portion of a solvent, the solvent comprised of water soluble hydrolyzed species; wherein at least some of the water soluble hydrolyzed species are the same as the water soluble hydrolyzed species obtainable from the hydrolysis of the biomass in the feedstock; B) maintaining the contact between the feedstock of the feedstock stream and the solvent at a temperature in the range of 20° C. to 200° C. for a time in the range of 5 minutes to 72 hours to create a hydrolyzed product from the biomass in the feedstock.
    Type: Grant
    Filed: November 28, 2016
    Date of Patent: September 5, 2017
    Assignee: Beta Renewables, S.p.A.
    Inventors: Marco Cotti Comettini, Paolo Torre, Francesco Cherchi, Alberto Riva, Simone Ferrero, Piero Ottonello, Mirko Garbero
  • Patent number: 9624516
    Abstract: A method produces a sugar liquid by repeating a sugar liquid production process including (1) to (3): (1) adding a filamentous fungus-derived cellulase to cellulose to perform primary hydrolysis; (2) adding a fresh filamentous fungus-derived cellulase to the hydrolysate in Step (1) to perform secondary hydrolysis; and (3) subjecting the hydrolysate in Step (2) to solid-liquid separation to obtain a sugar liquid, from which a recovered enzyme is obtained; wherein the recovered enzyme obtained in Step (3) is used for Step (1) of the next and later sugar liquid production processes.
    Type: Grant
    Filed: March 14, 2011
    Date of Patent: April 18, 2017
    Assignee: Toray Industries, Inc.
    Inventors: Hiroyuki Kurihara, Atsushi Minamino, Yuki Yamamoto, Katsushige Yamada
  • Patent number: 9611463
    Abstract: The present invention relates to variants of a parent cellobiohydrolase II. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: October 13, 2014
    Date of Patent: April 4, 2017
    Assignee: Novozymes, Inc.
    Inventor: Mark Wogulis
  • Patent number: 9593355
    Abstract: The present invention concerns a production of liquefied lignocellulosic substrate by enzymatic reaction. 10% to 40% by weight dry matter of pre-treated lignocellulosic substrate is contacted, with water and enzymes at 0.1 to 60 mg of enzymes per gram of cellulose for a period of 1 to 24 hours. Over time, at least the value of one of the rheological characteristics of the reaction medium is measured. If a reduction of the value is detected over time, the following step a) is carried out: a) the feeding flow rate of pre-treated lignocellulosic substrate is increased, with or without modification of the flow rate of enzymes and/or water; If an increase of the value is detected over time, a step b) is carried out: b) the feeding flow rate of water and/or enzymes is increased, with or without modification of the flow rate of pre-treated lignocellulosic substrate.
    Type: Grant
    Filed: November 29, 2012
    Date of Patent: March 14, 2017
    Assignee: IFP ENERGIES NOUVELLES
    Inventors: Sylvain Louret, Romain Rousset
  • Patent number: 9410960
    Abstract: A method of diagnosing galactosemia in blood samples from neonates by determining GAL-1-P concentrations before 5-7 days of life is disclosed. The removal of interfering compounds allows a more specific and therefore more accurate determination of GAL-IP levels in newborn screening for galactosemia using mass spectrometry. This is of major importance when investigating samples from children that have not yet achieved a steady state of GAL-IP (i.e. before 5-7 day of life).
    Type: Grant
    Filed: November 28, 2013
    Date of Patent: August 9, 2016
    Assignee: Statens Serum Institut
    Inventor: Arieh Sierra Cohen
  • Patent number: 9394548
    Abstract: Wheat bran culture mix is used to inoculate organic waste and produce an inoculated waste material, where the wheat bran culture mix includes soil derived microbes. The inoculated waste material is shredded to produce shredded inoculated waste material which is fermented the shredded inoculated waste material for at least 7 days. Contents from the fermenter are then transferred into a dewatering device to produce dewatered contents which are then separated into soluble and suspended products.
    Type: Grant
    Filed: March 9, 2012
    Date of Patent: July 19, 2016
    Inventor: Lawrence R. Green
  • Patent number: 9309548
    Abstract: A method of producing hydrolysate from cellulosic material by adding hydrothermally treated stillage or a fraction thereof to cellulosic material, treating the mixture of hydrothermally treated stillage and cellulosic material with at least one hydrolyzing enzyme, and hydrolyzing and converting complex carbohydrates in the cellulosic material. The hydrolysate produced by the method. Ethanol, organic acids, and organism metabolites produced by the method. Biomass produced by the method. A method of increasing sugar production rate and yield of sugars from cellulosic material by adding hydrothermally treated stillage or a fraction thereof to cellulosic material, treating the mixture of hydrothermally treated stillage and cellulosic material with at least one hydrolyzing enzyme, and hydrolyzing complex carbohydrates in the cellulosic material and forming sugars.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: April 12, 2016
    Assignee: Valicor, Inc
    Inventor: James Robert Bleyer
  • Patent number: 9260735
    Abstract: Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can convert feedstock materials to a sugar solution, which can then be fermented to produce ethanol. Biomass feedstock is dispersed in a liquid medium and then saccharified.
    Type: Grant
    Filed: March 25, 2014
    Date of Patent: February 16, 2016
    Assignee: Xyleco, Inc.
    Inventors: Marshall Medoff, Thomas Craig Masterman
  • Patent number: 9234140
    Abstract: A rotary machine type shock wave reactor suitable for thermal cracking of hydrocarbon-containing materials includes a casing, a rotor whose periphery contains an axial-flow blade cascade, and a directing rim, provided with at least two stationary vane cascades, adjoining an axial-flow rotor cascade, wherein the casing substantially encloses the periphery of the rotor and the directing rim. The cascades are configured to direct feedstock containing process stream to repeatedly pass the cascades in a helical trajectory while propagating within the duct between the inlet and exit and to generate stationary shock-waves to heat the feedstock. The axial-flow rotor cascade is configured to provide kinetic energy and add velocity to feedstock containing process stream, and the stationary vanes located downstream the rotor cascade are configured to reduce the velocity of the stream and convert kinetic energy into heat. The reactor may also process carbohydrate-and glyceride-based feedstock, and gaseous biomass matter.
    Type: Grant
    Filed: February 22, 2013
    Date of Patent: January 12, 2016
    Assignee: COOLBROOK OY
    Inventors: Jukka Seppala, Jyrki Hiltunen, Veli-Matti Purola
  • Patent number: 9212375
    Abstract: The invention relates to improvements in the production of ethanol and acetate by microbial fermentation, particularly to production of alcohols by microbial fermentation of a substrate comprising CO and the addition of an inorganic sulfur additive. It more particularly relates to the provision of an inorganic organic sulfur source to a fermentation system such that one or more microorganisms convert a substrate comprising CO to ethanol. In one aspect the invention uses a sulfur additive comprising inorganic sulfur compounds having a +2 to a +4 sulfur oxidation state that produces sulfur oxoanions and hydrosulfur oxoanions in an aqueous fermentation medium.
    Type: Grant
    Filed: July 11, 2012
    Date of Patent: December 15, 2015
    Assignee: COSKATA, LLC
    Inventors: Richard E. Tobey, Toby D. Allen, Peter Martin Lokken, Andrew James Heinen, Gengsheng Chen
  • Patent number: 9139857
    Abstract: A method is disclosed for the production of alcohol and other bioproducts hemicelluloses extracted from biomass prior to thermal conversion of the biomass to energy. The process can be integrated with the host plant process to minimize the energy loss from extracting hemicelluloses. Also disclosed is a stepwise enzymatic break down of cellulose fibers from a pulping operation which is performed with the redeployment of equipment and vessels contained within typical existing pulp and paper manufacturing mills. The preferred feedstock is highly delignified pulp from acid or alkaline pulping process or from bleaching stage.
    Type: Grant
    Filed: September 25, 2012
    Date of Patent: September 22, 2015
    Assignee: API Intellectual Property Holdings, LLC
    Inventors: Theodora Retsina, Vesa Pylkkanen
  • Patent number: 9085494
    Abstract: This invention provides processes and apparatus to convert biomass, including wood and agricultural residues, into low-ash biomass pellets for combustion, alone or in combination with another solid fuel. Some embodiments provide processes for producing low-ash biomass from cellulosic biomass, comprising providing an aqueous extraction solution with acetic acid; extracting the feedstock to produce an extract liquor containing soluble ash, hemicellulosic oligomers, acetic acid, dissolved lignin, and cellulose-rich solids; dewatering and drying the cellulose-rich, lignin-rich solids to produce a low-ash biomass; hydrolyzing the hemicellulosic oligomers to produce hemicellulosic sugars, wherein additional acetic acid is generated; removing a vapor stream comprising vaporized acetic acid from the extract; and recycling the vapor or its condensate to provide some starting acetic acid for the extraction solution. The disclosed processes can produce clean power from biomass.
    Type: Grant
    Filed: February 5, 2014
    Date of Patent: July 21, 2015
    Assignee: API Intellectual Property Holdings, LLC
    Inventors: Theodora Retsina, Vesa Pylkkanen
  • Publication number: 20150136120
    Abstract: Disclosed in this specification is a process for the improved hydrolysis of glucans to glucose wherein a portion of the glucose is removed from the process to improve the yield of glucose.
    Type: Application
    Filed: January 9, 2013
    Publication date: May 21, 2015
    Inventors: Paolo Torre, Simone Ferrero, Piero Ottonello, Liliane Tonet Rensi, Francesco Cherchi, Dario Giordano