Aspartic Acid (asparaginic Acid); Asparagine Patents (Class 435/109)
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Publication number: 20100112647Abstract: An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium.Type: ApplicationFiled: October 15, 2009Publication date: May 6, 2010Inventors: Yoshihiko Hara, Keita Fukui, Yoshinori Tajima, Kazue Kawamura, Yoshihiro Usuda, Kazuhiko Matsui
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Publication number: 20100055748Abstract: An L-amino acid can be produced by culturing an L-amino acid-producing bacterium which belongs to the Enterobacteriaceae family and which has been modified so that the expression of a yggG gene is enhanced.Type: ApplicationFiled: August 25, 2009Publication date: March 4, 2010Inventors: Masahito Taya, Motomu Nishioka, Yoshihiro Ojima, Mizuho Komaki, Shintaro Iwatani
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Publication number: 20100047878Abstract: An L-amino acid is produced by culturing a microorganism belonging to the family Enterobacteriaceae having an L-amino acid-producing ability and modified so that glycerol dehydrogenase and dihydroxyacetone kinase activities are increased, in a medium containing glycerol as a carbon source to produce and accumulate an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.Type: ApplicationFiled: August 24, 2009Publication date: February 25, 2010Inventors: Yuri Nagai, Kazuyuki Hayashi, Takuji Ueda, Yoshihiro Usuda, Kazuhiko Matsui
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Publication number: 20100040649Abstract: The invention provides improved rtTA and single chain rtTA variants and uses thereof for inducible expression of a nucleic acid of interest. Nucleic acid sequences comprising an improved rtTA and/or sc rtTA sequence according to the invention are also provided, as well as vectors, replicons and cells comprising such nucleic acid sequences.Type: ApplicationFiled: November 17, 2006Publication date: February 18, 2010Applicant: Academisch CentrumInventors: Benjamin Berkhout, Atze T. Das
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Publication number: 20090325242Abstract: The invention relates to mutants and alleles of the zwf gene of coryneform bacteria, which encode variants of the Zwf subunit of glucose 6-phosphate dehydrogenase (EC: 1.1.1.49), and to processes for preparing amino acids, in particular L-lysine and L-tryptophan, by using bacteria which harbor said alleles.Type: ApplicationFiled: July 28, 2009Publication date: December 31, 2009Applicant: Degussa AGInventors: Brigitte Bathe, Natalie Schischka, Georg Thierbach
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Publication number: 20090317876Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to have glycerol kinase in which feedback inhibition by fructose-1,6-bisphosphate is desensitized, thereby having enhanced ability to utilize glycerol.Type: ApplicationFiled: June 22, 2009Publication date: December 24, 2009Inventors: Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Marina Evgenievna Sheremet'eva, Yulia Aleksandrovna Ovodova, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov
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Patent number: 7608191Abstract: A process of separating suspended solids from a fermentation liquor by subjecting the liquor to a solids-liquid separation stage, wherein the fermentation liquor is produced in a fermentation process for the production of a fermentation product, and which liquor comprises lignin, wherein the solids-liquid separation stage is assisted by a treatment system, characterized in that the treatment system comprises an anionic polymer, with the proviso that the treatment system and does not include a cationic polymer having an intrinsic viscosity (IV) of at least 4 dl/g.Type: GrantFiled: January 20, 2005Date of Patent: October 27, 2009Assignee: Ciba Specialty Chemicals Water Treatments Ltd.Inventors: Jonathan Hughes, Rajesh Mistry, Kenneth Charles Symes
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Patent number: 7598062Abstract: A process for the production of L-amino acids, in particular L-threonine, in which the following steps are carried out: (a) fermentation of the microorganisms of the family Enterobacteriaceae producing the desired L-amino acid, in which the fruR gene or nucleotide sequences coding therefor are attenuated, in particular are switched off, (b) enrichment of the L-amino acid in the medium or in the cells of the bacteria, and (c) isolation of the L-amino acid.Type: GrantFiled: February 9, 2006Date of Patent: October 6, 2009Assignee: Evonik Degussa GmbHInventors: Mechthild Rieping, Thomas Hermann
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Publication number: 20090239267Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the cpxR gene.Type: ApplicationFiled: January 22, 2008Publication date: September 24, 2009Inventors: Konstantin Vyacheslavovich Rybak, Aleksandra Yurievna Skorokhodova, Elvira Borisovna Voroshilova, Tatyana Viktorovna Leonova
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Publication number: 20090239269Abstract: A bacterium which belongs to the Enterobacteriaceae family and has an ability to produce an L-amino acid such as L-lysine, L-threonine and L-tryptophan and is modified to enhance glutamic acid decarboxylase activity is cultured in a medium to produce and accumulate the L-amino acid in the medium or cells of the bacterium. Then, the L-amino acid is collected from the medium or the cells.Type: ApplicationFiled: April 9, 2009Publication date: September 24, 2009Inventors: Yoshinori Tajima, Shintaro Iwatani, Yoshihiro Usuda, Kazuhiko Matsui
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Publication number: 20090239266Abstract: The present invention provides a method for producing a non-aromatic L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to attenuate expression of the csrA gene.Type: ApplicationFiled: July 31, 2007Publication date: September 24, 2009Inventors: Andrey Yurievich Gulevich, Danila Vadimovich ZIMENKOV, Elena Vitalievna KLYACHKO, Tatyana Viktorovna LEONOVA, Yury Ivanovich Kozlov, Vitaly Grigorievich PARASKEVOV
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Patent number: 7588923Abstract: A process for producing high yields of enantioselective amino acids and chiral amines by reacting a keto acid or ketone and an amino acid donor in the presence of a transaminase biocatalyst to produce a keto acid by-product and an amino acid or amine product. Further reacting the keto acid by-product with a peroxide to increase the yield of additional amino acid or amine product.Type: GrantFiled: February 29, 2008Date of Patent: September 15, 2009Assignee: Richmond Chemical CorporationInventors: Ian Fotheringham, Nicholas Oswald
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Publication number: 20090226980Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to attenuate expression of the kefB gene.Type: ApplicationFiled: November 5, 2007Publication date: September 10, 2009Inventors: Dmitriy Vladimirovich Filippov, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
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Patent number: 7582215Abstract: A process of separating suspended solids from a fermentation liquor by subjecting the liquor to a solids-liquid separation stage, wherein the fermentation liquor is produced in a fermentation process for the production of a fermentation product, which liquor comprises water, lignin and BOD, wherein the solids liquid separation stage is assisted by a treatment system, characterised in that the treatment system comprises either, (i) a cationic polymer having an intrinsic viscosity (IV) of at least 4 dl/g at a dose of above 2 kg/tonne based on dry weight of suspension, or (ii) a cationic polymer having an intrinsic viscosity (IV) of at least 4 dl/g and, (iii) an anionic polymer, and/or (iv) a cationic polymer of intrinsic viscosity of below 4 dl/g and a cationic charge density of at least 3 meq/g and/or (v) inorganic coagulants and/or (vi) charged microparticulate material.Type: GrantFiled: July 30, 2003Date of Patent: September 1, 2009Assignee: Ciba Specialty Chemicals Water Treatments Ltd.Inventors: Jonathan Hughes, Kenneth Charles Symes
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Patent number: 7582444Abstract: Process for improving the separation efficiency of residual solid matter from the liquid phase of an aqueous acid hydrolysate of a naturally occurring polysaccharide comprising dissolved sugars, and residual acid wherein a flocculating agent(s) is added to the aqueous mixture in an effective amount, and a process of producing fermentation products comprising the steps of, (i) hydrolysing a particulate polysaccharide based plant derived material in an acid medium, and thereby forming an aqueous mixture comprising dissolved sugar and solid matter, (ii) subjecting the aqueous mixture to one or more separation stages in which solid matter are removed from the aqueous phase, (iii) adjusting the pH of the obtained aqueous phase to a pH of at least 4, (iv) fermenting the dissolved sugars of the aqueous phase by a microorganism to produce a fermentation product, (v) isolating the fermentation product, wherein in at least one separation stage in step (ii) a flocculating agent is added to the aqueous mixture in an effeType: GrantFiled: July 28, 2003Date of Patent: September 1, 2009Assignee: Ciba Specialty Chemicals Water Treatments Ltd.Inventor: Jonathan Hughes
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Publication number: 20090215129Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to attenuate expression of the leuO gene.Type: ApplicationFiled: September 4, 2007Publication date: August 27, 2009Inventors: Konstantin Vyacheslavovich Rybak, Elvira Borisovna Voroshilova, Tatyana Viktorovna Leonova, Yury Ivanovich Kozlov
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Publication number: 20090209011Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to enhance expression of at least one gene of the fucPIKUR operon.Type: ApplicationFiled: December 7, 2007Publication date: August 20, 2009Inventors: Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Marina Evgenievna Sheremet'eva, Aleksandra Yurievna Skorokhodova, Vitaly Grigorievich Paraskevov
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Publication number: 20090203090Abstract: A method for producing an L-amino acid is described, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine, L-tryptophan, or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of a wild-type alcohol dehydrogenase encoded by the adhE gene or a mutant alcohol dehydrogenase which is resistant to aerobic inactivation.Type: ApplicationFiled: January 15, 2009Publication date: August 13, 2009Inventors: LEONID ROMANOVICH PTITSYN, IRINA BORISOVNA ALTMAN, VERONIKA ALEKSANDROVNA KOTLIAROVA, OLGA NIKOLAEVNA MOKHOVA, TATYANA ABRAMOVNA YAMPOLSKAYA, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov, MASARU TERASHITA, YOSHIHIRO USUDA, KAZUHIKO MATSUI
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Publication number: 20090170169Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the rspAB operon.Type: ApplicationFiled: December 30, 2008Publication date: July 2, 2009Inventors: Dmitriy Vladimirovich Filippov, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
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Publication number: 20090137011Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the rcsA gene.Type: ApplicationFiled: November 26, 2008Publication date: May 28, 2009Inventors: Dmitriy Vladimirovich Filippov, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
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Publication number: 20090098621Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of a gene coding for sRNA.Type: ApplicationFiled: September 18, 2008Publication date: April 16, 2009Inventors: Konstantin Vyacheslavovich Rybak, Aleksandra Yurievna Skorokhodova, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner, Tatyana Viktorovna Leonova, Yury Ivanovich Kozlov, Takuji Ueda, Vitaly Grigorievich Paraskevov
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Publication number: 20090087886Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the sfmACDFH-fimZ cluster and/or the fimZ gene.Type: ApplicationFiled: October 17, 2008Publication date: April 2, 2009Inventors: Dmitriy Vladimirovich Filippov, Vera Georgievna Doroshenko, Aleksandra Yurievna Skorokhodova, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
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Publication number: 20090081738Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to attenuate expression of the bolA gene.Type: ApplicationFiled: July 31, 2007Publication date: March 26, 2009Inventors: Dmitriy Vladimirovich Filippov, Elvira Borisovna Voroshilova, Tatyana Viktorovna Leonova, Yuri Ivanovich Kozlov, Vitaly Grigorievich Paraskevov
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Publication number: 20090068711Abstract: The invention relates to a method for the production of L-amino acids by fermentation of recombinant microorganisms of the Enterobacteriaceae family, characterized in that a) the microorganisms producing the desired L-amino acid and wherein the lamB-gene or nucleotide sequence coding for the gene product maltoporin is amplified, particularly overexpressed, are cultivated in a medium in conditions enabling the desired L-amino acid to be enriched in the medium or in cells, and b) the desired L-amino acid is isolated, wherein constituents of the fermentation broth and/or biomass remain in the entirety thereof or in parts thereof (=0 bis 100%) in the isolated product or are fully removed.Type: ApplicationFiled: July 5, 2005Publication date: March 12, 2009Applicant: Degussa GmbHInventor: Mechthild Rieping
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Publication number: 20090036576Abstract: The present invention relates to fermentation processes for the production of cyanophycin in a microorganism whereby a plant-derived nitrogen source is converted by the microorganism into cyanophycin. The plant-derived nitrogen source preferably is a process stream being obtained in the processing of agricultural crops such as e.g., a by-product in the processing of starch from agricultural crops like corn, potato or cassave. The invention further relates to processes for the conversion of cyanophycin into a variety of compounds including e.g., ornithine, 1,4-butanediamine, n-alkyl amino alcohols, acrylonitrile, as well as cyanophycin derived functionalised poly(aspartic acid)s wherein the arginine residues have been functionalised to ornithine, (N-L-arginino)succinate, N-phospho-L-arginine or agmantine and the lysine residues have been functionalised to N6-hydroxy-L-lysine, 2,5-diaminohexanoate, N6-(L-1,3-dicarboxypropyl), pentanediamine, 5-aminopentanamide or N6-acetyl-L-lysine.Type: ApplicationFiled: March 6, 2006Publication date: February 5, 2009Inventors: Yasser Abdel Kader Elbahloul, Elinor Lindsey Scott, Andreas Mooibroek, Johan Pieter Marinus Sanders, Martin Obst, Alexander Steinbuchel
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Publication number: 20080293109Abstract: The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products.Type: ApplicationFiled: July 14, 2008Publication date: November 27, 2008Applicant: Novozymes, Inc.Inventors: Randy Berka, Joel Cherry
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Patent number: 7455997Abstract: A process of producing fermentation product comprising the steps of, (i) forming an acidified suspension of particulate plant derived material comprising a first polysaccharide which is more readily hydrolysable and a second polysaccharide which is more difficult to hydrolysable, (ii) allowing the first polysaccharide to undergo hydrolysis by action of the acid at a temperature of at least 50° C.Type: GrantFiled: July 28, 2003Date of Patent: November 25, 2008Assignee: Ciba Specialty Chemicals Water Treatments LtdInventor: Jonathan Hughes
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Patent number: 7432085Abstract: In a method for producing an L-amino acid by culturing a microorganism having an ability to produce an L-amino acid in a medium to produce and accumulate the L-amino acid in the medium and collecting the L-amino acid from the medium, a Gram-negative bacterium having the Entner-Doudoroff pathway and modified so that 6-phosphogluconate dehydratase activity or 2-keto-3-deoxy-6-phosphogluconate aldolase activity, or activities of the both are enhanced is used as the microorganism.Type: GrantFiled: November 18, 2005Date of Patent: October 7, 2008Assignee: Ajinomoto Co., Inc.Inventors: Yoshihiko Hara, Hiroshi Izui, Takahiro Asano, Yasuyuki Watanabe, Tsuyoshi Nakamatsu
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Publication number: 20080233617Abstract: The present invention relates to increasing the product of aspartate or aspartate-derived metabolites by boosting the activity of the glyoxylate shunt. This is accomplished by increasing the activity of glyoxylate shunt specific enzymes and decreasing the activity of reactions consuming glyoxylate and its precursors.Type: ApplicationFiled: August 11, 2006Publication date: September 25, 2008Inventors: Rainer Figge, Gwenaelle Bestel-Corre, Celine Raynaud, Philippe Soucaille
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Patent number: 7399616Abstract: The invention relates to a process for preparing a Na-acyl-L-arginine ester, derived from fatty acids and esterified dibasic amino acids, according to the following formula (I), where: X— is Br—, Cl—, or HSO4— R1: is linear alkyl chain from an saturated fatty acid, or hydroxy-acid from 8 to 14 atoms of carbon bonded to the ?-amino acid group through amidic bond. R2: is a linear or branched alkyl chain from 1 to 18 carbon atoms or aromatic. R3: is formula (II), where n can be from 0 to 4, from the appropriate organic acid and alcohol. The process is catalyzed by a hydrolase, more in particular a protease, the protease papain from <i> Carica papaya being highly suitable </i>. In order for the esterification reaction to run as wanted, the process is performed in a low-water-content organic medium. When the highly suitable papain from Carica papaya is used, a water activity of between 0.03 and 0.5 is selected.Type: GrantFiled: February 1, 2002Date of Patent: July 15, 2008Assignee: Laboratorios Miret, S.A.Inventors: Joan Seguer Bonaventura, Xavier Rocabayera Bonvila
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Publication number: 20080153138Abstract: A method for producing an L-amino acid, such as L-phenylalanine and L-threonine, is provided using an Escherichia bacterium, wherein the L-amino acid productivity of said bacterium is enhanced by enhancing the activity of the protein encoded by the yedA gene. A further method is provided for producing a lower alkyl ester of ?-L-aspartyl-L-phenylalanine by producing L-phenylalanine by cultivating an Escherichia coli bacterium in a medium, wherein said bacterium has the ability to produce L-phenylalanine, and synthesizing the lower alkyl ester of ?-L-aspartyl-L-phenylalanine from aspartic acid, or a derivative thereof, and the produced L-phenylalanine.Type: ApplicationFiled: June 12, 2007Publication date: June 26, 2008Inventors: Vitaliy Arkadyevich Livshits, Maria Viacheslavovna Vitushkina, Mikhail Markovich Gusyatiner, Mikhail Kharisovich Ziyatdinov, Valery Zavenovich Akhverdian, Ekaterina Alekseevna Savrasova, Vera Georgievna Doroshenko, Sergey Vladimirovich Mashko
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Patent number: 7300776Abstract: An L-amino acid-producing strain of Escherichia coli is bred by modifying an Escherichia coli K12 strain or a derivative thereof so as to become resistant to L-valine and have an ability to produce one or more L-amino acids selected from the group consisting of L-tryptophan, L-phenylalanine, L-lysine, L-tyrosine, L-glutamic acid, L-histidine, L-cysteine, and L-proline.Type: GrantFiled: April 25, 2005Date of Patent: November 27, 2007Assignee: Ajinomoto Co., Inc.Inventors: Hisao Ito, Yuji Joe
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Patent number: 7160703Abstract: The present invention provides nucleotide sequences from Coryneform bacteria which code for the PtsI protein and a process for the fermentative preparation of amino acids using bacteria in which the ptsI gene is enhanced.Type: GrantFiled: June 13, 2003Date of Patent: January 9, 2007Assignee: Degussa AGInventors: Bettina Moeckel, Stephan Hans, Natalie Schischka, Walter Pfefferle
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Patent number: 7157251Abstract: The present invention relates to the processes of racemization and deprotection of special N-protected amino acids in the acylase/racemase system for the total conversion of special N-protected racemic amino acids into optically pure amino acids.Type: GrantFiled: September 2, 2003Date of Patent: January 2, 2007Assignee: Degussa AGInventors: Andreas Bommarius, Karlheinz Drauz, Stefan Verseck
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Patent number: 7129059Abstract: A process for preparation of a compound with enhanced optical purity is disclosed wherein a mixture of the enantiomers of a chiral amine is brought into contact with an enzyme having peptide deformylase activity with a bivalent metal ion as a cofactor.Type: GrantFiled: February 8, 2006Date of Patent: October 31, 2006Assignee: DSM IP Assets B.V.Inventors: Peter Jan Leonard Mario Quaedflieg, Theodorus Sonke, Adolf Fritiz Volker Wagner
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Patent number: 7037690Abstract: In a method for producing an L-amino acid by culturing a microorganism having an ability to produce an L-amino acid in a medium to produce and accumulate the L-amino acid in the medium and collecting the L-amino acid from the medium, a Gram-negative bacterium having the Entner-Doudoroff pathway and modified so that 6-phosphogluconate dehydratase activity or 2-keto-3-deoxy-6-phosphogluconate aldolase activity, or activities of the both are enhanced is used as the microorganism.Type: GrantFiled: March 26, 2003Date of Patent: May 2, 2006Assignee: Ajinomoto Co., Inc.Inventors: Yoshihiko Hara, Hiroshi Izui, Takahiro Asano, Yasuyuki Watanabe, Tsuyoshi Nakamatsu
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Patent number: 7018817Abstract: Process for the preparation of a compound with enhanced optical purity wherein a mixture of the enantiomers of a chiral compound of formula 1 wherein: R1 represents an alkyl or an aryl group R2 represents H, an alkyl or an aryl group Y represents an alkyl group, an aryl group, (CH2)nCOOH, (CH2)n—COOR, (CH2)n—CONRR?, CH2OH, or C?N wherein R and R? independently represent H, an alkyl or aryl group, and n represents 0 or 1, is brought into contact with an enzyme having peptide deformylase activity with a bivalent metal ion as a cofactor wherein the metal is chosen from the groups 5–11 of the periodic system, or for the preparation of a formylated compound with enhanced optical purity from a mixture of the enantiomers of the corresponding not formulated chiral compound in the presence of a formylation agent. Preferably the peptide deformylase is chosen from the class EC 3.5.2.27 or EC 3.5.1.31, and contains the sequences of (I) HEXXH, (ii) EGCLS and (iii) GXGXAAXQ.Type: GrantFiled: December 17, 1999Date of Patent: March 28, 2006Assignee: DSM IP Assets B.V.Inventors: Peter Jan Leonard Mario Quaedflieg, Theodorus Sonke, Adolf Fritz Volker Wagner
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Patent number: 6953677Abstract: A novel rec-L-N-carbamoylase from Arthrobacter aurescens and its method of use for producing L-amino acids. The recombinantly produced L-carbamoylase is unexpectedly stable, so that an industrial method of producing L-amino acids can be established with it, in contrast to previously known L-carbamoylases.Type: GrantFiled: January 15, 2002Date of Patent: October 11, 2005Assignees: Degussa AG, Universitat StuttgartInventors: Josef Altenbuchner, Ralf Mattes, Markus Pietzsch, Christoph Syldatk, Anja Wiese, Burkard Wilms
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Patent number: 6919190Abstract: The present invention provides a method of increasing the productivity of a microorganism by improving the assimilation of carbon dioxide. Specifically, the invention provides a polypeptide having phosphoenolpyruvate carboxylase activity which does not require acetyl coenzyme A for activation and is desensitized to feedback inhibition by aspartic acid, and to genes coding for this polypeptide. A gene encoding a PEP carboxylase that is not regulated by acetyl-CoA or aspartic acid can improve carbon flow from the three carbon intermediate PEP to the four carbon intermediate OAA, contribute to compounds derived from OAA, and increase amino acid biosynthesis. The invention further provides recombinant DNA molecules containing these genes, bacteria transformed with these genes, and a method of producing amino acids using the transformed bacteria.Type: GrantFiled: October 18, 2001Date of Patent: July 19, 2005Assignee: Archer-Daniels-Midland CompanyInventors: P. John Rayapati, Corey M. Crafton
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Patent number: 6821760Abstract: This invention relates to a method for producing L-aspartic acid comprising treating an ammonium fumarate solution with aspartase to generate an ammonium L-aspartate solution; adding fumaric acid to the solution; and then crystallizing L-aspartic acid from the solution, wherein fumaric acid is added to the ammonium L-aspartate solution after the solution has been heated to 50 to 130° C. in an amount 0.4 to 0.8 times the total amount of fumarate and the L-aspartate contained therein in terms of mole, and the resultant mixture is once turned into a homogeneous solution by applying thereto a shearing force, and then L-aspartic acid is deposited therefrom, or wherein the solution is cooled at a rate of 0.1-5° C./min from the temperature at which fumaric acid is added thereto to the temperature at which crystallized L-aspartic acid is separated therefrom, to thereby deposit L-aspartic acid.Type: GrantFiled: September 29, 1999Date of Patent: November 23, 2004Assignee: Nippon Shokubai Co., Ltd.Inventors: Masaharu Mukouyama, Shinzo Yasuda, Satomi Komatsuzaki
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Patent number: 6808914Abstract: The invention relates to nucleotide sequences coding for the accDA gene and to a process for the preparation of L-amino acids, especially L-lysine, by fermentation using corynebacteria in which the accDA gene is amplified.Type: GrantFiled: December 21, 2001Date of Patent: October 26, 2004Assignee: Degussa AGInventors: Yvonne Tilg, Bernhard Eikmanns, Lothar Eggeling, Hermann Sahm, Bettina Möckel
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Patent number: 6806070Abstract: Use of an extract of bacterium from the family Pseudomonadaceae in the production of cosmetic compositions in particular for combating ageing of the skin.Type: GrantFiled: April 18, 2002Date of Patent: October 19, 2004Assignee: L'OrealInventors: Richard Martin, Pascal Hilaire, Nathalie Pineau, Lionel Breton
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Patent number: 6730518Abstract: Disclosed is a method of reducing photooxidation or air oxidation in susceptible materials such as food, plastics or pharmaceuticals comprising mixing the material with an antioxidation composition comprising at least one amino acid, at least one metal ion, and at least one carboxylic acid in an amount effective to reduce photooxidation in the material.Type: GrantFiled: September 25, 2000Date of Patent: May 4, 2004Assignee: Natura, Inc.Inventors: Joel I. Dulebohn, Ronald J. Carlotti
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Publication number: 20040014180Abstract: The invention relates to a method for the microbial production of metabolic products, polynucleotides from coryne-form bacteria and use thereof. According to the invention, by means of said method and polynucleotides it is possible to influence the synthesis of ATP in a controlled manner and also to control the synthesis of metabolic products. The invention relates to genes from Corynebacterium glutamicum coding for cytochrome aa3 oxidase and the cytochrome bc1 complex. The monocistronic ctaD gene codes for a 65 kDa protein, the primary structure of which displays all the typical properties of the sub-unit I of cytochrome aa3 oxidase. The genes which code for the sub-unit III of the cytochrome aa3 (ctaE) and the three characteristic sub-units of the cytochrome bc1 complex (qcrABC) are arranged in a group with the sequence ctaE-qcrCAB.Type: ApplicationFiled: August 1, 2003Publication date: January 22, 2004Inventors: Michael Bott, Axel Niebisch, Brigitte Bathe, Achim Marx, Thomas Hermann
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Patent number: 6656710Abstract: The present invention relates to the processes of racemization and deprotection of special N-protected amino acids in the acylase/racemase system for the total conversion of special N-protected racemic amino acids into optically pure amino acids.Type: GrantFiled: October 11, 2001Date of Patent: December 2, 2003Assignee: Degussa AGInventors: Andreas Bommarius, Karlheinz Drauz, Stefan Verseck
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Publication number: 20030219881Abstract: The invention relates to coryneform bacteria which have, in addition to at least one copy, present at the natural site (locus), of an open reading frame (ORF), gene or allele which codes for the synthesis of a protein or an RNA, in each case a second, optionally third or fourth copy of this open reading frame (ORF), gene or allele at in each case a second, optionally third or fourth site in a form integrated into the chromosome and processes for the preparation of chemical compounds by fermentation of these bacteria.Type: ApplicationFiled: February 5, 2003Publication date: November 27, 2003Applicant: Degussa AGInventors: Bathe Brigitte, Kreutzer Caroline, Mockel Bettina, Thierbach Georg
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Publication number: 20030203455Abstract: The present invention provides a novel D-aminoacylase, as well as method for producing a D-amino acid using the same. In order to achieve the above objective , the present inventors have succeeded in purifying heat-stable D-aminoacylase from microorganisms belonging to the genus Streptomyces by combining various purification methods. Furthermore, the present inventors found that the purified heat-stable D-aminoacylase is useful in industrial production of D-amino acids. By utilizing the heat-stable D-aminoacylase, it is possible to readily and efficiently produce the corresponding D-amino acids from N-acetyl-DL-amino acids (for example, N-acetyl-DL-methionine, N-acetyl-DL-valine, N-acetyl-DL-tryptophan, N-acetyl-DL-phenylalanine, N-acetyl-DL-alanine, N-acetyl-DL-leucine, and so on).Type: ApplicationFiled: February 7, 2003Publication date: October 30, 2003Applicant: Daicel Chemical Industries, Ltd., a Japan corporationInventors: Shinji Tokuyama, Akinobu Matsuyama
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Patent number: 6617127Abstract: The invention relates to the synthesis of aspartame involving enzymatic deformylation of an N-formyl-&agr;-L-aspartyl-L-phenylalanine compound by treatment with an enzyme having formylmethionyl peptide deformylase activity and having as a co-factor group 5 to 11 bivalent metal ions. The invention also relates to selective preparation and recovery of aspartame from a mixture of N-formyl-&agr;- and &bgr;-L-aspartyl-L-phenylalanine compounds by treatment with such enzyme. And finally, the invention relates to one-pot enzymatic synthesis of aspartame from N-formyl-L-aspartic acid and L- or D,L-phenylalanine methyl ester involving an enzymatic deformylation reaction simultaneously with an enzymatic coupling reaction, as well as to one-pot di- or oligopeptide synthesis by simultaneous enzymatic coupling and deformylation reactions in general.Type: GrantFiled: June 22, 2001Date of Patent: September 9, 2003Assignee: Holland Sweetener Company, V.O.F.Inventors: Peter J. L. M. Quaedflieg, Theodorus Sonke, Adolf F. V. Wagner
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Patent number: 6559280Abstract: The invention relates to novel compounds comprising a ubiquitination recognition element and a protein binding element. The invention also relates to the use of said compounds for modulating the level and/or activity of a target protein. The compounds are useful for the treatment of disease such as infections, inflammatory conditions, cancer and genetic diseases. The compounds are also useful as insecticides and herbicides.Type: GrantFiled: June 14, 2001Date of Patent: May 6, 2003Assignee: Proteinix, Inc.Inventors: John H. Kenten, Steven F. Roberts
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Patent number: 6551803Abstract: A process is described for purifying an amino acid-containing solution by means of electrodialysis, wherein an amino acid-containing solution is employed which is obtained from the fermentation for producing at least one amino acid.Type: GrantFiled: May 1, 2002Date of Patent: April 22, 2003Assignee: BASF AktiengesellschaftInventors: Andreas Fischer, Christoph Martin, Jürgen Müller