Preparing Alpha Or Beta Amino Acid Or Substituted Amino Acid Or Salts Thereof Patents (Class 435/106)
  • Patent number: 11124814
    Abstract: Host cells that are engineered to produce benzylisoquinoline alkaloid (BIAs) precursors, such as norcoclaurine (NC) and norlaudanosoline (NL), are provided. The host cells may have one or more engineered modifications selected from: a feedback inhibition alleviating mutation in a enzyme gene; a transcriptional modulation modification of a biosynthetic enzyme gene; an inactivating mutation in an enzyme; and a heterologous coding sequence. Also provided are methods of producing a BIA of interest or a precursor thereof using the host cells and compositions, e.g., kits, systems etc., that find use in methods of the invention.
    Type: Grant
    Filed: November 3, 2014
    Date of Patent: September 21, 2021
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Michael Shareef Siddiqui, Christina D. Smolke
  • Patent number: 11104925
    Abstract: The present disclosure relates to a microorganism producing L-lysine and a method for producing L-lysine by using the same. More specifically, the present disclosure relates to a microorganism of the genus Corynebacterium, which is modified such that the activity of a protein involved in cell wall hydrolysis is inactivated in comparison with the endogenous activity thereof; and a method for producing L-lysine using the same.
    Type: Grant
    Filed: June 27, 2016
    Date of Patent: August 31, 2021
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Peter Lee, Hyung Joon Kim, Hyang Choi, Song Gi Ryu, Sang Mok Lee
  • Patent number: 11034985
    Abstract: The present invention relates to a method for producing L-methionine in which a microorganism is cultured in the presence of L-homoserine and methyl mercaptan, a salt of the same or dimethyl disulfide whereby the L-methionine is accumulated in the culture medium.
    Type: Grant
    Filed: November 1, 2016
    Date of Patent: June 15, 2021
    Assignee: EVONIK OPERATIONS GMBH
    Inventors: Ines Ochrombel, Daniel Fischer, Brigitte Bathe, Marleen Hasselmeyer, Michael Hampel, Joanne Pedall
  • Patent number: 10995316
    Abstract: The present disclosure provides compositions and methods for making and using methanotrophic proline auxotrophs.
    Type: Grant
    Filed: May 13, 2016
    Date of Patent: May 4, 2021
    Assignee: CALYSTA, INC.
    Inventor: Jana Stumpe
  • Patent number: 10961554
    Abstract: The present disclosure relates to a novel promoter and a method for producing L-amino acids using the promoter, and more specifically, to a novel polynucleotide having promoter activity, a vector and a microorganism of the genus Corynebacterium comprising the polynucleotide, a method for producing L-amino acids and a fermented composition using the microorganism, and a fermented composition.
    Type: Grant
    Filed: March 27, 2019
    Date of Patent: March 30, 2021
    Assignee: CJ Cheiljedang Corporation
    Inventors: Ji Yeon Lee, Jin Sook Chang, Hyung Joon Kim, Byoung Hoon Yoon, Sun Hyoung Choi, Yunjung Choi
  • Patent number: 10899782
    Abstract: The present invention relates to a method for separating sialylated oligosaccharides from a fermentation broth in which they are produced by a genetically modified microorganism The separation comprises the steps of: i) ultrafiltration; ii) nano-filtration; iii) optionally, activated charcoal treatment; and iv) treatment with strong anion and/or cation exchange resin.
    Type: Grant
    Filed: March 7, 2017
    Date of Patent: January 26, 2021
    Assignee: GLYCOM A/S
    Inventors: Martin Matwiejuk, Nikolay Khanzhin, Pierre Chassagne, Markus Jondelius Hederos
  • Patent number: 10889843
    Abstract: The present invention relates to an L-lysine-producing microorganism of the genus Corynebacterium and a method for producing L-lysine using the same.
    Type: Grant
    Filed: July 27, 2016
    Date of Patent: January 12, 2021
    Assignee: CJ Cheiljedang Corporation
    Inventors: Hyo Jeong Byun, Yoon Hee Chung, Hyung Joon Kim, Sun Young Lee, Hyun Koo Nam, Sun Mi Park, Sang Mok Lee
  • Patent number: 10752930
    Abstract: A method for producing a protein of interest on a manufacturing scale is based on integration, by homologous recombination, of the DNA encoding the protein of interest into a bacterial cell's genome at a pre-selected site. The manufacturing scale production of recombinant proteins is in the fed-batch mode, semi-continuous or in a chemostat.
    Type: Grant
    Filed: May 12, 2017
    Date of Patent: August 25, 2020
    Assignees: Boehringer Ingelheim RCV GmbH & Co KG, Sandoz AG
    Inventors: Gerald Striedner, Johann Huber, Daniela Reinisch
  • Patent number: 10689677
    Abstract: A method is used for the fermentative production of L-lysine using bacteria of the species Corynebacterium glutamicum, having the ability to excrete L-lysine and containing in their chromosome a mutated NCgl2816 polynucleotide. Further, the method is used for cultivating the bacteria in a suitable medium under suitable conditions, and accumulating said L-lysine in the suitable medium to form an L-lysine containing fermentation broth.
    Type: Grant
    Filed: September 18, 2019
    Date of Patent: June 23, 2020
    Assignee: Evonik Operations GmbH
    Inventors: Frank Schneider, Georg Thierbach, Thomas Bekel, Kornelia Voβ
  • Patent number: 10682398
    Abstract: Vaccine compositions including a yeast comprising an immunostimulatory polypeptide and optionally an antigenic polypeptide are provided herein. The immunostimulatory polypeptide and the antigenic polypeptide are expressed or displayed on the surface of the yeast vaccine composition. Methods of using the vaccine composition to vaccinate subjects are also provided.
    Type: Grant
    Filed: May 3, 2017
    Date of Patent: June 16, 2020
    Assignees: THE TEXAS A&M UNIVERSITY SYSTEM, THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS
    Inventors: Olivia B. Faulkner, Lisa Bielke, Leona Nicole Calhoun, Luc Berghman, Billy Hargis
  • Patent number: 10676512
    Abstract: The present disclosure relates to a novel modified RNA polymerase sigma factor A (SigA) polypeptide; a polynucleotide encoding the same; a microorganism containing the polypeptide; and a method for producing L-lysine using the microorganism.
    Type: Grant
    Filed: September 4, 2015
    Date of Patent: June 9, 2020
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Lan Huh, Kwang Ho Lee, Hyung Joon Kim, Jun Ok Moon, Song-Gi Ryu
  • Patent number: 10647976
    Abstract: The expression plasmid vectors comprise a polynucleotide sequence encoding Ldc2 polypeptide, a fragment, and/or a mutant. A backbone plasmid is capable of autonomous replication in a host cell. The host cell is not a P. aeruginosa cell. Transformants are transformed with expression plasmid vector. The transformants are not P. aeruginosa. Mutant host cells comprise a polynucleotide sequence encoding Ldc2 polypeptide, a fragment and/or a mutant that has been integrated into the host cell chromosome. A polypeptide, a fragment and/or a mutant comprise Ldc2. A non-naturally occurring polynucleotide, and/or a mutant encodes polypeptide comprising Ldc2. Biobased cadaverine is produced using the transformants and the biobased cadaverine is prepared by the method. Polyamides are formed using the biobased cadaverine and compositions.
    Type: Grant
    Filed: June 26, 2014
    Date of Patent: May 12, 2020
    Assignees: Cathay Biotech Inc., CIBT America Inc
    Inventors: Howard Chou, Naiqiang Li, Xiucai Liu
  • Patent number: 10626426
    Abstract: The present invention relates to a microorganism of the genus Corynebacterium having an ability to produce L-arginine, and a method of producing L-arginine using the same.
    Type: Grant
    Filed: October 13, 2015
    Date of Patent: April 21, 2020
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Hyun Ae Bae, Han Hyoung Lee, Min Gyeong Kang, Jong Hyun Kim, Hye Won Kim
  • Patent number: 10604781
    Abstract: The present disclosure relates to the use of an amino acid dehydrogenase in combination with a cofactor regenerating system comprising a ketoreductase. In particular embodiments, the process can be used to prepare L-tert-leucine using a leucine dehydrogenase.
    Type: Grant
    Filed: December 14, 2018
    Date of Patent: March 31, 2020
    Assignee: Codexis, Inc.
    Inventors: Fabien Louis Cabirol, Steven J. Collier, Thomas Daussmann, Naga K. Modukuru
  • Patent number: 10604744
    Abstract: The present disclosure provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The disclosure also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral amine compounds.
    Type: Grant
    Filed: April 4, 2018
    Date of Patent: March 31, 2020
    Assignee: Codexis, Inc.
    Inventors: Martina Quintanar-Audelo, Ellen Eberhard, Jovana Nazor, Derek Smith, Cuixia Wang
  • Patent number: 10544435
    Abstract: The present invention relates to the provision of genetically modified microbial cells, such as yeast cells with an improved ability for producing L-ornithine and its derivatives. Overproduction of L-ornithine is obtained in the first place by the down-regulation or attenuation of specially selected genes, wherein said genes encode enzymes involved in the L-ornithine consumption and/or degradation pathways. Further L-ornithine production ability is improved by down-regulation, attenuation, deletion or overexpression of specially selected genes, wherein said genes encode enzymes and/or proteins involved in the L-ornithine ‘acetylated derivatives cycle’, L-glutamate synthesis pathways, subcellular trafficking, TCA cycle, pyruvate carboxylation pathway, respiratory electron-transport chain, and the carbon substrates' assimilation machinery. The invention additionally provides a method to produce L-ornithine with said modified eukaryotic cells.
    Type: Grant
    Filed: March 10, 2016
    Date of Patent: January 28, 2020
    Assignee: CHRYSEA LIMITED
    Inventors: Jiufu Qin, Anastasia Krivoruchko, Florian David, Bo Jiang, Jens Nielsen
  • Patent number: 10501763
    Abstract: The present disclosure relates to a microorganism producing O-acetylhomoserine with high efficiency and a method for producing O-acetylhomoserine and L-methionine using the microorganism. The present disclosure provides a microorganism producing O-acetylhomoserine having an enhanced activity of a protein which is predicted to export O-acetylhomoserine, and a method for producing O-acetylhomoserine and L-methionine using the microorganism.
    Type: Grant
    Filed: June 5, 2015
    Date of Patent: December 10, 2019
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Jee Yeon Bae, Hyun Ah Kim, Yong Uk Shin, So Young Kim, Sang Kyoum Kim, Kwang Ho Na, Ju Hee Seo, Sung Kwang Son, Hye Ryun Yoo, Jin Geun Choi
  • Patent number: 10472656
    Abstract: A fermentation process for fermenting a starch hydrolysate containing 60-98 weight percent of glucose based on total carbohydrate and 0.3-5% weight percent of isomaltose based on total carbohydrate to a non-ethanol fermentation product is carried out. The method comprises: a) forming a fermentation broth containing the starch hydrolysate and a genetically modified microorganism containing i) an exogenous gene encoding transporter capable of transporting isomaltose ii) an exogenous gene encoding an enzyme capable of converting isomaltose to glucose; and b) fermenting the starch hydrolysate in the fermentation broth to produce a non-ethanol fermentation product. This fermentation process may be carried out as a single step fermentation or a batch process. The genetically modified microorganism may contain an exogenous gene encoding an enzyme that catalyzes the formation of a product other than ethanol.
    Type: Grant
    Filed: June 18, 2015
    Date of Patent: November 12, 2019
    Assignee: CARGILL, INCORPORATED
    Inventors: Keith M. Brady, Christopher K. Miller
  • Patent number: 10450548
    Abstract: The present disclosure provides non-naturally occurring c1 microorganisms useful for the production of lactate and related compositions, as well as methods for the biologically production of lactate. In specific embodiments, the present disclosure provides non-naturally occurring methanotrophic bacteria which are useful for producing lactate from c1 substrates.
    Type: Grant
    Filed: June 18, 2014
    Date of Patent: October 22, 2019
    Assignee: CALYSTA, INC.
    Inventors: Renee M. Saville, Sungwon Lee, Drew D. Regitsky, Sol M. Resnick, Joshua A. Silverman
  • Patent number: 10385368
    Abstract: Compositions and methods for producing malonate in recombinant host cells in order to increase malonate titer, yield, and/or productivity are provided. Recombinant host cells comprising nucleic acids encoding MAE1 transport proteins that increase production of malonate by the host cell and vectors for expressing MAE1 transport proteins that increase production of malonate by the host cell are also disclosed.
    Type: Grant
    Filed: June 24, 2015
    Date of Patent: August 20, 2019
    Assignee: Lygos, Inc.
    Inventor: Jeffrey A. Dietrich
  • Patent number: 10385367
    Abstract: The invention features methods and compositions relating to cells that have been engineered to reduce or eliminate proteins having enzymatic activity that interfere with the expression of a metabolic product.
    Type: Grant
    Filed: June 1, 2010
    Date of Patent: August 20, 2019
    Inventors: Jeffrey C. Way, Joseph H. Davis
  • Patent number: 10329591
    Abstract: The present invention is related to a recombinant microorganism optimized for the fermentative production of methionine and/or its derivatives, wherein in said recombinant strain, the methionine efflux is enhanced by overexpressing the homologous logous genes of ygaZ and ygaH genes from Escherichia coli. It is also related to a method for optimizing the fermentative production of methionine or its derivatives comprising the steps of: a. culturing a recombinant microorganism wherein in said microorganism, the methionine efflux is enhanced by overexpressing the ygaZH homologous genes of ygaZ and ygaH genes from Escherichia coli, in an appropriate culture medium comprising a fermentable source of carbon and a source of sulphur, and b. recovering methionine and/or its derivatives from the culture medium.
    Type: Grant
    Filed: August 31, 2015
    Date of Patent: June 25, 2019
    Assignee: Evonik Degussa GmbH
    Inventors: Rainer Figge, Laurence Dumon-Seignovert, Perrine Vasseur, Wanda Dischert
  • Patent number: 10316297
    Abstract: The present disclosure relates to an acetohydroxy acid synthase variant in which the feedback inhibition to L-valine is released, a polynucleotide encoding the acetohydroxy acid synthase variant, an expression vector including the polynucleotide, a microorganism producing L-valine including the acetohydroxy acid synthase variant, and a method for producing L-valine using the microorganism.
    Type: Grant
    Filed: August 5, 2015
    Date of Patent: June 11, 2019
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Ae Ji Jeon, Byeong Cheol Song, Jong Hyun Kim, Hye Won Kim, Ji Hye Lee
  • Patent number: 10301657
    Abstract: Modified or recombinant microorganisms are provided herein that can be used to produce one or more amino acids, including, for example, methionine or one or more methionine biosynthetic pathway-derived intermediates or one or more methionine-based products.
    Type: Grant
    Filed: June 16, 2016
    Date of Patent: May 28, 2019
    Assignee: MOgene Green Chemicals LLC
    Inventors: Abhay Kumar Singh, Himadri Pakrasi, Ganesh Murthy Kishore
  • Patent number: 10266831
    Abstract: Provided are microorganisms that catalyze the synthesis of chemicals and biochemicals from a methanol, methane and/or formaldehyde. Also provided are methods of generating such organisms and methods of synthesizing chemicals and biochemicals using such organisms.
    Type: Grant
    Filed: December 12, 2016
    Date of Patent: April 23, 2019
    Assignee: The Regents of the University of California
    Inventors: James C. Liao, Igor Bogorad
  • Patent number: 10253341
    Abstract: The present invention relates to improving the enzymatic activity of Escherichia coli O157:H7-derived sugar isomerase by molecular mechanics and site-directed mutagenesis. More particularly, the present invention relates to a sugar isomerase having an enzymatic activity improved by mutation, a nucleic acid molecule encoding the same, a vector comprising the nucleic acid molecule, a transformant comprising the vector, a mutant of the sugar isomerase, and a method for producing L-gulose using the improved sugar isomerase.
    Type: Grant
    Filed: June 13, 2014
    Date of Patent: April 9, 2019
    Assignee: KONKUK UNIVERSITY INDUSTRIAL COOPERATION CORP.
    Inventors: Jung-Kul Lee, Chandra Sujan Sigdel, Jinglin Li, Tae-Su Kim
  • Patent number: 10167460
    Abstract: Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.
    Type: Grant
    Filed: July 24, 2014
    Date of Patent: January 1, 2019
    Assignee: DANISCO US INC
    Inventors: Maria Foukaraki, Ronaldus Wilhelmus Hommes, Thijs Kaper, Bradley R. Kelemen, Slavko Kralj, Suzanne E. Lantz, Igor Nikolaev, Wilhelmus Van Der Kley, Johan Franciscus Thomas Van Lieshout, Sander Van Stigt Thans
  • Patent number: 10125378
    Abstract: There is provided an additive for a bioethanol fermentation process comprising a polyoxyalkylene compound (A) having a Griffin's HLB value in the range of 0 to 6, a polyoxyalkylene polyol (B) and a base oil (C) that is liquid at 25° C. The compound (A) is preferably a mixture of a compound represented by a general formula (1) and a compound represented by a general formula (2). In the formula, R1 and R3 represent alkyl or alkenyl, R2 and R4 represent a hydrogen atom or a monovalent organic group, AO represents oxyalkylene having a carbon number of 3 to 18, a reaction residue of glycidol, a reaction residue of an alkyl glycidyl ether or a reaction residue of an alkenyl glycidyl ether, EO represents oxyethylene, m and n are 1 to 100, and p is 3 to 10.
    Type: Grant
    Filed: January 8, 2016
    Date of Patent: November 13, 2018
    Assignee: SAN NOPCO LTD.
    Inventors: Hironori Nagamatsu, Tsuyoshi Ando
  • Patent number: 10113191
    Abstract: Disclosed are a recombinant microorganism having enhanced L-amino acid productivity, wherein the recombinant microorganism is transformed to have removed or decreased activity of at least one of adenosine deaminase and AMP nucleosidase, and a method of producing an L-amino acid using the recombinant microorganism. The use of the recombinant microorganism may enable the production of the L-amino acid in a highly efficient manner.
    Type: Grant
    Filed: March 17, 2015
    Date of Patent: October 30, 2018
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Eun Sung Koh, Su Yon Kwon, Kwang Ho Lee, Ji Sun Lee, Juno Jang, Keun Cheol Lee, Hyeong Pyo Hong
  • Patent number: 10087468
    Abstract: An object of the present invention is to provide an additive that can improve the production efficiency. The present invention is an additive for a bioethanol fermentation process comprising a polyoxyalkylene compound (A) having a Griffin's HLB value in the range of 0 to 6 and a polyoxyalkylene polyol (B). The compound (A) is preferably a mixture of a compound represented by a general formula (1) and a compound represented by a general formula (2). R1O-(AO)m-R2 (1). R3O-(AO)n-(EO)p-R4 (2). R1 and R3 represent alkyl or alkenyl, R2 and R4 represent a hydrogen atom or a monovalent organic group, AO represents oxyalkylene having a carbon number of 3 to 18, or a reaction residue of glycidol, an alkyl glycidyl ether or alkenyl glycidyl ether, EO represents oxyethylene, m and n are 1 to 100, and p is 3 to 10.
    Type: Grant
    Filed: February 19, 2014
    Date of Patent: October 2, 2018
    Assignee: SAN NOPCO LTD.
    Inventors: Hironori Nagamatsu, Tsutomu Miyata
  • Patent number: 10066244
    Abstract: Processes for producing ethanol comprise saccharifying cellulosic material with a cellulolytic enzyme composition and fermenting the saccharified cellulosic material with a fermenting microorganism to produce ethanol. The fermenting organism is Saccharomyces cerevisiae CIBTS1260 (deposited under Accession No. NRRL Y-50973 at the Agricultural Research Service Culture Collection (NRRL), Illinois 61604 U.S.A.) or a fermenting organism that has properties that the same or about the same as that of Saccharomyces cerevisiae CIBTS1260).
    Type: Grant
    Filed: September 22, 2015
    Date of Patent: September 4, 2018
    Assignee: Novozymes A/S
    Inventors: Liuyang Diao, Kate Brandon Sutton, Yu Jiang, Sheng Yang, Yingmiao Liu, Fenghui Qian
  • Patent number: 10053698
    Abstract: The present invention relates to an E. coli mutant strain having enhanced L-threonine productivity, which is obtained by introducing the permease of Corynebacterium origin, and to method of producing L-threonine using the E. coli mutant strain.
    Type: Grant
    Filed: April 25, 2014
    Date of Patent: August 21, 2018
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Hyung Joon Kim, Su Yon Kwon, Eun Sung Koh, Ji Sun Lee, Keun Cheol Lee, Young Bin Hwang, Hyeong Pyo Hong
  • Patent number: 10023610
    Abstract: The present invention relates to the field of molecular biochemistry and medicine, and in particular to ligands comprising modified amino acid residues, targeting the amyloid-? peptide associated with Alzheimer's disease for prevention of aggregation, neurotoxicity and use thereof as drugs for treatment of Alzheimer's disease.
    Type: Grant
    Filed: January 16, 2015
    Date of Patent: July 17, 2018
    Assignee: AlphaBeta AB
    Inventors: Roger Strömberg, Dmytro Honcharenko, Jyotirmoy Maity, Alok Juneja, Firoz Roshan Kurudenkandy, Jenny Presto, Lisa Dolfe, André Fisahn, Jan Johansson, Lennart Nilsson
  • Patent number: 10006063
    Abstract: The invention relates to the field of producing succinate by E. coli fermentation. Specifically, the invention provides an engineered recombinant E. coli for producing succinate, wherein said E. coli contains one or more of the following modifications: a) enhanced activity of the protein(s) encoded by the gene(s) involved in pentose phosphate pathway (PPP), b) enhanced activity of the protein encoded by sthA gene, and optionally c) mutant lpdA gene. The invention also relates to use of the engineered recombinant E. coli for producing succinate, and a method of using the engineered recombinant E. coli for producing succinate.
    Type: Grant
    Filed: May 23, 2014
    Date of Patent: June 26, 2018
    Assignee: TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCES
    Inventors: Xueli Zhang, Xinna Zhu, Hongtao Xu, Zaigao Tan
  • Patent number: 9963685
    Abstract: The present disclosure provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The disclosure also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral amine compounds.
    Type: Grant
    Filed: February 27, 2017
    Date of Patent: May 8, 2018
    Assignee: Codexis, Inc.
    Inventors: Martina Quintanar-Audelo, Ellen Eberhard, Jovana Nazor, Derek Smith, Cuixia Wang
  • Patent number: 9938546
    Abstract: The present invention relates to a modified polynucleotide encoding aspartate kinase (EC:2.7.2.4; hereinafter, referred to as LysC), transketolase (EC:2.2.1.1; hereinafter, referred to as Tkt) or pyruvate carboxylase (EC:6.4.1.1; hereinafter, referred to as Pyc), in which the initiation codon is substituted with ATG, a vector including the same, a microorganism transformed with the vector, and a method for producing L-lysine using the same.
    Type: Grant
    Filed: January 30, 2017
    Date of Patent: April 10, 2018
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Kwang Ho Lee, Sang Jo Lim, Jun Ok Moon, Jae Woo Jang, Su Jin Park, Sang Hee Park
  • Patent number: 9920342
    Abstract: A novel process for the preparation of L-threo-dihydroxyphenylserine (Droxidopa) is described. It comprises of enantioselective hydrolysis of racemic (DL)-threo-N-acetyl-3-(3,4-methylenedioxyphenyl)-serine using commercially available L-amino acylase from Aspergillus sp. (EC 3.5.1.14) in the presence of cobalt ions, to obtain (L)-threo-3-(3,4-methylenedioxyphenyl)-serine followed by dealkylation to obtain Droxidopa. Protecting the amino group of (L)-threo-3-(3,4-methylenedioxyphenyl)-serine using either benzyloxycarbonyl or phthaloyl group before dealkylation followed by deprotection of the amino group results in obtaining Droxidopa in high yields and purity.
    Type: Grant
    Filed: January 24, 2017
    Date of Patent: March 20, 2018
    Inventors: Satchandra Kiran Divi, Mysore Aswatha Narayana Rao, Ponnekanti Purnachandra Rao, Shaik Nowshuddin
  • Patent number: 9873721
    Abstract: A fluorescent fusion protein is provided. The fluorescent fusion protein of the invention comprises a first fluorescent protein with first emission wavelength, a second fluorescent protein with second excitation wavelength and second emission wavelength, and a linker peptide linking the first fusion protein and the second fusion protein, wherein the first emission wavelength overlaps the second excitation wavelength so that the second fluorescent protein exhibits the second emission wavelength after receiving energy. The fluorescent fusion protein of the invention exhibits a stronger fluorescence in the absence of bilivirdin (BV).
    Type: Grant
    Filed: November 17, 2014
    Date of Patent: January 23, 2018
    Assignee: National Yang-Ming University
    Inventors: Yi-Jang Lee, Bo-Sheng Wang, Liang-Ting Lin, C.Allen Chang, Jyh-Cheng Chen
  • Patent number: 9873898
    Abstract: The present invention provides a method for producing an L-amino acid by fermentation using a bacterium of the family Enterobacteriaceae. The bacterium can belong to the genus Escherichia, which has been modified to attenuate expression of a phosphate transporter-encoding gene, such as the pitA gene or pitB gene.
    Type: Grant
    Filed: March 28, 2016
    Date of Patent: January 23, 2018
    Assignee: AJINOMOTO CO., INC.
    Inventors: Tatyana Mikhailovna Kuvaeva, Evgeniya Aleksandrovna Polyakova, Natalia Viktorovna Stoynova
  • Patent number: 9803221
    Abstract: The present invention provides for the manipulation of carbon flux in a recombinant host cell to increase the formation of desirable products. The invention relates to cellulose-digesting organisms that have been genetically modified to allow the production of ethanol at a high yield by redirecting carbon flux at key steps of central metabolism.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: October 31, 2017
    Assignees: Enchi Corporation, Dartmouth College
    Inventors: Yu Deng, Daniel G. Olson, Johannes Pieter van Dijken, Arthur J. Shaw, IV, Aaron Argyros, Trisha Barrett, Nicky Caiazza, Christopher D. Herring, Stephen R. Rogers, Frank Agbogbo
  • Patent number: 9796991
    Abstract: The present invention relates to recombinant bacteria producing L-amino acid, in which the recombinant bacteria has reduced expression of the glucose-6-phosphate isomerase gene pgi and improved expression of the glucose-6-phosphate dehydrogenase gene -opcA than the starting bacteria, where the starting bacterium is a bacterial strain that can accumulate target amino acid(s) and preferably, the amino acid is L-histidine.
    Type: Grant
    Filed: February 4, 2015
    Date of Patent: October 24, 2017
    Assignee: INSTITUTE OF MICROBIOLOGY, CHINESE ACADEMY OF SCIENCES
    Inventors: Tingyi Wen, Xiuling Shang, Yun Zhang, Shuwen Liu, Yong Liang, Yu Zhang
  • Patent number: 9777301
    Abstract: Disclosed are methods for producing optically active amino acids and amines. According to the methods, ?-keto acids are generated as reaction intermediates, and as a result, ?-transaminase-catalyzed kinetic resolution of racemic amino acids or amines as racemic amine compounds enables the production of optically active amine compounds without the need to use expensive ?-keto acids as starting materials. Therefore, the optically active amine compounds are produced at greatly reduced costs. In addition, the optically active amine compounds have high enantiomeric excess.
    Type: Grant
    Filed: December 13, 2013
    Date of Patent: October 3, 2017
    Assignee: INDUSTRY-ACADEMIC COOPERATION FOUNDATION, YONSEI UNIVERSITY
    Inventors: Jong-Shik Shin, Eul-Soo Park, M. Shaheer Malik
  • Patent number: 9765370
    Abstract: A process for producing alanine, including culturing a cell under aerobic conditions in an aqueous phase in the presence of an inorganic nitrogen source, and then contacting the aqueous phase and the cell cultured in the aqueous phase with a hydrophobic organic phase. The cell is a prokaryotic or a lower eukaryotic cell and expresses a recombinant alanine dehydrogenase.
    Type: Grant
    Filed: March 25, 2013
    Date of Patent: September 19, 2017
    Assignee: Evonik Degussa GmbH
    Inventors: Hans-Georg Hennemann, Steffen Schaffer, Mirja Wessel, Markus Pötter, Jan Christoph Pfeffer, Thomas Haas, Jasmin Corthals, Eva-Maria Eckl, Silvana Roeder, Wolfgang Kroutil, Arne Skerra
  • Patent number: 9714439
    Abstract: The present disclosure relates to the use of an amino acid dehydrogenase in combination with a cofactor regenerating system comprising a ketoreductase. In particular embodiments, the process can be used to prepare L-tert-leucine using a leucine dehydrogenase.
    Type: Grant
    Filed: June 16, 2016
    Date of Patent: July 25, 2017
    Assignee: Codexis, Inc.
    Inventors: Fabien Louis Cabirol, Steven J. Collier, Thomas Daussmann, Naga K. Modukuru
  • Patent number: 9695433
    Abstract: The present invention relates to isolated polypeptides having beta-xylosidase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: November 22, 2012
    Date of Patent: July 4, 2017
    Assignee: Novozymes Inc.
    Inventors: Yu Zhang, Ye Liu, Junxin Duan, Lan Tang, Brett McBrayer
  • Patent number: 9663803
    Abstract: A genetically engineered yeast cell capable of producing lactate having increased TPI activity, a method of preparing the yeast cell, and a method of producing lactate by using the yeast cell.
    Type: Grant
    Filed: November 13, 2014
    Date of Patent: May 30, 2017
    Assignee: SAMSUNG ELECTRONICS CO., LTD
    Inventors: Juyoung Lee, Changduk Kang, Soyoung Lee, Youngkyoung Park, Jiyoon Song, Seunghyun Lee, Kwangmyung Cho
  • Patent number: 9593354
    Abstract: The present invention relates to a modified polynucleotide encoding aspartate kinase (EC:2.7.2.4; hereinafter, referred to as LysC), transketolase (EC:2.2.1.1; hereinafter, referred to as Tkt) or pyruvate carboxylase (EC:6.4.1.1; hereinafter, referred to as Pyc), in which the initiation codon is substituted with ATG, a vector including the same, a microorganism transformed with the vector, and a method for producing L-lysine using the same.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: March 14, 2017
    Assignee: CJ Cheiljedang Corporation
    Inventors: Kwang Ho Lee, Sang Jo Lim, Jun Ok Moon, Jae Woo Jang, Su Jin Park, Sang Hee Park
  • Patent number: 9567616
    Abstract: A target substance can be efficiently produced by culturing, in a medium, a coryneform bacterium in which the activity of a PTS protein relating to fructose uptake is reduced or lost as compared with a parent strain and the bacterium can produce the target substance, allowing the target substance to form and accumulate in a culture; and collecting the target substance from the culture.
    Type: Grant
    Filed: February 9, 2012
    Date of Patent: February 14, 2017
    Assignee: KYOWA HAKKO BIO CO., LTD.
    Inventors: Tetsuro Ujihara, Tetsuya Abe, Makoto Yagasaki
  • Patent number: 9551018
    Abstract: The present invention relates to the production of optically pure secondary amines, which can be used as intermediate products in a synthesis of for instance pharmaceutical products.
    Type: Grant
    Filed: June 16, 2015
    Date of Patent: January 24, 2017
    Assignee: LONZA AG
    Inventors: Karen Robins, Uwe Scheuer, Matthias Hohne
  • Patent number: 9518278
    Abstract: Provided are microorganisms that catalyze the synthesis of chemicals and biochemicals from a methanol, methane and/or formaldehyde. Also provided are methods of generating such organisms and methods of synthesizing chemicals and biochemicals using such organisms.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: December 13, 2016
    Assignee: The Regents of the University of California
    Inventors: James C. Liao, Igor Bogorad