Citrulline; Arginine; Ornithine Patents (Class 435/114)
  • Patent number: 11365400
    Abstract: Disclosed is a modified microorganism producing putrescine or ornithine, and a method for producing putrescine or ornithine using the same.
    Type: Grant
    Filed: October 19, 2020
    Date of Patent: June 21, 2022
    Assignee: CJ Cheiljedang Corporation
    Inventors: Su Jin Park, Young Lyeol Yang, Hye Won Um, Hong Xian Li, Kyoung Min Lee, Baek Seok Lee, Hyo Hyoung Lee, Hee Kyoung Jung
  • Patent number: 11053525
    Abstract: The present invention relates to a recombinant microorganism for producing putrescine or ornithine, and a method for producing putrescine or ornithine using the same. Specifically, the present invention relates to a microorganism of the genus Corynebacterium capable of producing putrescine or ornithine, in which an activity of the transcriptional regulator of sugar metabolism (SugR) is weakened, an activity of the citrate synthase (GltA) is enhanced, or both are applied; and a method for producing putrescine or ornithine using the same.
    Type: Grant
    Filed: March 29, 2016
    Date of Patent: July 6, 2021
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Hee Kyoung Jung, Hye Won Um, Hong Xian Li, Su Jin Park, Young Lyeol Yang, Kyoung Min Lee, Hyo Hyoung Lee
  • Patent number: 10316322
    Abstract: This invention provides high growth capacity strains of auxotrophic Escherichia coli and methods for generating thereof. The high growth capacity strains express a complementing auxotrophic plasmid that allows the strain to grow in the absence of the auxotrophic amino acid. Also, provided herein is a method for preparing a bacterial cell extract of a high growth capacity strain of auxotrophic Escherichia coli for use in an in vitro protein expression.
    Type: Grant
    Filed: June 30, 2015
    Date of Patent: June 11, 2019
    Assignee: Sutro Biopharma, Inc.
    Inventors: Dan Groff, Patrick Rivers, Stuart Bussell, Alexander Steiner
  • Patent number: 10125383
    Abstract: Disclosed is a method for producing L-citrulline using recombinant Corynebacterium crenatum cells as whole-cell biocatalysts. The present invention provides a recombinant C. crenatum that expresses an exogenous arginine deiminase gene from Lactobacillus brevis. The recombinant C. crenatum SDNN403 is used as biocatalysts for converting L-arginine to produce L-citrulline. Using the method of the invention, the concentration of L-citrulline reached 301.4 g/L after a 48 hr conversion reaction, and the molar conversion rate reached 99.9%.
    Type: Grant
    Filed: January 22, 2018
    Date of Patent: November 13, 2018
    Assignee: Jiangnan University
    Inventors: Zhiming Rao, Meizhou Wang, Meijuan Xu, Xian Zhang, Taowei Yang
  • Publication number: 20150140614
    Abstract: The invention relates to an isolated polynucleotide having promoter activity, a variant of the promoter of the gap gene coding for glyceraldehyde-3-phosphate dehydrogenase; and to a microorganism which produces and/or secretes a fine chemical, the microorganism including the isolated polynucleotide having promoter activity, which enables various genes to be overexpressed in comparison with the particular starting strain; and to a process for preparing fine chemicals using the microorganism.
    Type: Application
    Filed: January 28, 2015
    Publication date: May 21, 2015
    Applicant: EVONIK DEGUSSA GMBH
    Inventors: ALEXANDER RETH, BRIGITTE BATHE, STEPHAN HANS, WILFRIED CLAES
  • Publication number: 20150118720
    Abstract: At least one amino acid selected from the group consisting of L-arginine, L-citrulline and L-ornithine can be produced with high efficiency by culturing a coryneform bacterium in a culture medium to produce and accumulate the amino acid in a culture and then collecting the amino acid from the culture, wherein the coryneform bacterium is produced by introducing a deletion, substitution or addition of at least one nucleotide into at least one gene which is present in chromosomal DNA of a parent strain and encodes a protein having an L-arginine, L-citrulline or L-ornithine uptake activity so that the activity of uptake of the amino acid in the coryneform bacterium is reduced or lost compared with that in the parent strain, and can produce the amino acid.
    Type: Application
    Filed: April 12, 2013
    Publication date: April 30, 2015
    Applicant: Kyowa Hakko Bio Co., Ltd.
    Inventor: Satoshi Mitsuhashi
  • Patent number: 8969048
    Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.
    Type: Grant
    Filed: May 2, 2014
    Date of Patent: March 3, 2015
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yury Ivanovich Kozlov, Akito Chinen, Hiroshi Izui, Yoshihiko Hara, Hisashi Yasueda, Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Joanna Yosifovna Katashkina
  • Publication number: 20150056666
    Abstract: The invention relates to an isolated polynucleotide having promoter activity, a variant of the promoter of the gap gene coding for glyceraldehyde-3-phosphate dehydrogenase; and to a microorganism which produces and/or secretes a fine chemical, the microorganism including the isolated polynucleotide having promoter activity, which enables various genes to be overexpressed in comparison with the particular starting strain; and to a process for preparing fine chemicals using the microorganism.
    Type: Application
    Filed: November 4, 2014
    Publication date: February 26, 2015
    Applicant: Evonik Degussa GmbH
    Inventors: Alexander RETH, Brigitte Bathe, Stephan Hans, Wilfried Claes
  • Publication number: 20150056665
    Abstract: A method of producing a chemical includes culturing cells in a culture solution in a fermentor to ferment a feedstock to produce a chemical; supplying the culture solution containing the chemical produced in the culturing to a plurality of separation membrane units arranged in parallel; filtering the culture solution supplied in the supplying to separate a permeate containing the chemical; refluxing a retentate that is not filtered in the filtering to the fermentor; and supplying a gas containing oxygen to the plurality of separation membrane units while a supply amount is changed to at least two different values to perform scrubbing, wherein the supply amount and supply time of the gas containing oxygen supplied in the culturing and the supplying the gas are set so that a kLa value is within a predetermined range from an optimal kLa value for the cells cultured in the culturing.
    Type: Application
    Filed: March 27, 2013
    Publication date: February 26, 2015
    Inventors: Satoko Kanamori, Hideki Sawai, Norihiro Takeuchi
  • Patent number: 8951759
    Abstract: A process for the fermentative preparation of L-ornithine using microorganisms characterized by an increased export of the amino acid.
    Type: Grant
    Filed: February 20, 2014
    Date of Patent: February 10, 2015
    Assignee: Evonik Degussa GmbH
    Inventors: Wilfried Claes, Robert Gerstmeir
  • Publication number: 20150010962
    Abstract: A method for producing a basic substance by fermentation comprising culturing a microorganism having an ability to produce the basic substance in a liquid medium contained in a fermentation tank to produce and accumulate the basic substance in the medium, wherein amount of sulfate and/or chloride ions used as counter ions of the basic substance is reduced by adjusting total ammonia concentration in the medium to be within a specific concentration range during at least a part of the total period of culture process.
    Type: Application
    Filed: September 11, 2014
    Publication date: January 8, 2015
    Applicant: AJINOMOTO CO., INC.
    Inventors: Ryo Takeshita, Shinichi Sugimoto
  • Publication number: 20140377815
    Abstract: The present invention relates to a method of producing carbamoyl phosphate, the method comprising reacting ammonia, ATP, bicarbonate and CO2, or a hydrated form thereof, in a composition in the presence of a carbamate kinase, wherein the ammonia and CO2, or hydrated form thereof, are converted to carbamate in a chemical reaction and the carbamate and ATP are converted to carbamoyl phosphate in an enzyme-catalysed reaction by the carbamate kinase, and wherein the pH of the composition is about 8 to about 12. The invention also relates to methods of producing urea.
    Type: Application
    Filed: June 15, 2012
    Publication date: December 25, 2014
    Inventors: James Edward Hennessy, Amy Philbrook, Daniel Miles Bartkus, Christopher John Easton, Colin Scott, John G. Oakeshott, Hye-Kyung Kim, Melissa Jane Latter
  • Patent number: 8883460
    Abstract: The present invention relates to a polynucleotide that is active to an acetyl glutamate synthase and acetyl ornithinase which are associated with ornithine or arginine biosynthesis from Corynebacterium glutamicum. The present invention also relates to a polypeptide encoded by said polynucleotide, a recombinant vector comprising said polynucleotide, to a transformant obtained by introducing said recombinant vector to a host microorganism for producing L-ornithine or L-arginine, and transforming the recombinant vector, and to a method for producing L-ornithine or L-arginine by culturing said transformant. The activity of the transformant of the present invention to an acetyl glutamate synthase and acetyl ornithinase is increased as compared to an intrinsic activity, and thus L-ornithine or L-arginine can be produced, at a high yield rate, from the transformant of the present invention.
    Type: Grant
    Filed: December 29, 2010
    Date of Patent: November 11, 2014
    Assignee: CJ Cheiljedang Corp.
    Inventors: Jin-Man Cho, Hye-Won Kim, Ji-Hye Lee, Jae-Yong Cho
  • Publication number: 20140315261
    Abstract: The present invention provides a method for producing L-amino acids by fermentation using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to attenuate expression of the yjjK gene.
    Type: Application
    Filed: April 23, 2014
    Publication date: October 23, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Natalia Viktorovna Stoynova, Valery Vasilievich Samsonov, Natalia Sergeevna Eremina, Evgeniya Aleksandrovna Polyakova
  • Patent number: 8859242
    Abstract: A method for producing a basic substance by fermentation comprising culturing a microorganism having an ability to produce the basic substance in a liquid medium contained in a fermentation tank to produce and accumulate the basic substance in the medium, wherein amount of sulfate and/or chloride ions used as counter ions of the basic substance is reduced by adjusting total ammonia concentration in the medium to be within a specific concentration range during at least a part of the total period of culture process.
    Type: Grant
    Filed: February 24, 2012
    Date of Patent: October 14, 2014
    Assignee: Ajinomoto Co., Inc.
    Inventors: Ryo Takeshita, Shinichi Sugimoto
  • Publication number: 20140287472
    Abstract: There is disclosed a method for producing L-amino acid, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of D-xylose permease.
    Type: Application
    Filed: June 11, 2014
    Publication date: September 25, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Ekaterina Alekseevna Savrasova, Valeriy Zavenovich Akhverdian, Elena Vitalievna Klyachko, Sergei Vladimirovich Mashko, Vera Georgievna Doroshenko, Larisa Gotlibovna Airikh, Tatyana Viktorovna Leonova, Mikhail Markovich Gusyatiner, Elvira Borisovna Voroshilova, Yury Ivanovich Kozlov, Yoshihiko Hara, Takuji Ueda
  • Publication number: 20140242643
    Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.
    Type: Application
    Filed: May 2, 2014
    Publication date: August 28, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Yury Ivanovich Kozlov, Akito Chinen, Hiroshi Izui, Yoshihiko Hara, Hisashi Yasueda, Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Joanna Yosifovna Katashkina
  • Publication number: 20140162325
    Abstract: The present invention provides a method for producing L-amino acid using a bacterium belonging to the family Enterobacteriaceae, particularly a motile bacterium belonging to the genus Escherichia, Enterobacter or Pantoea, wherein the bacterium has been modified so that expression of at least one gene of the flagella formation and motility cascade is enhanced.
    Type: Application
    Filed: February 11, 2014
    Publication date: June 12, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Irina Borisovna Altman, Tatyana Abramovna Yampolskaya, Leonid Romanovich Ptitsyn
  • Patent number: 8741608
    Abstract: A process for the fermentative preparation of L-ornithine using microorganisms characterized by an increased export of the amino acid.
    Type: Grant
    Filed: March 29, 2011
    Date of Patent: June 3, 2014
    Assignee: Evonik Degussa GmbH
    Inventors: Wilfried Claes, Robert Gerstmeir
  • Patent number: 8722370
    Abstract: The present invention provides a method for producing L-amino acids, such as L-arginine, L-citrulline, and L-lysine, using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to attenuate expression of one or more genes, such as the pepA, pepB, and pepD genes.
    Type: Grant
    Filed: November 28, 2012
    Date of Patent: May 13, 2014
    Assignee: Ajinomoto Co., Inc.
    Inventors: Dmitriy Vladimirovich Filippov, Tatyana Viktorovna Leonova, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner, Mika Moriya, Yuri Nagai, Keiko Noguchi
  • Patent number: 8703446
    Abstract: The present invention provides a method for producing L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to enhance the expression of the bssR gene, which encodes a regulator of biofilm through signal secretion.
    Type: Grant
    Filed: January 7, 2013
    Date of Patent: April 22, 2014
    Assignee: Ajinomoto Co., Inc.
    Inventors: Mikhail Yurievich Kiryukhin, Mikhail Markovich Gusyatiner
  • Publication number: 20140051132
    Abstract: The present invention provides a method for producing an L-amino acid belonging to the glutamate family, using a coryneform bacterium which has been modified so that expression of one or more gene(s) of the NCgl_2067-NCgl_2065 operon in said bacterium is/are attenuated.
    Type: Application
    Filed: October 3, 2013
    Publication date: February 20, 2014
    Applicant: AJINOMOTO CO., INC.
    Inventors: Svetlana Alekseevna Samsonova, Viktor Vasilievich Samsonov, Yulia Georgievna Rostova, Mikhail Markovich Gusyatiner
  • Patent number: 8633000
    Abstract: Processes for producing a suitable purity grade of L-Citrulline are disclosed. The processes can include contacting crude L-Citrulline in an aqueous solution with an adsorptive medium at a temperature above approximately 50° C. and below the temperature of denaturement for the L-Citrulline for an interval sufficient to remove at least one contaminant from the L-Citrulline. The processes can also include concentrating the dissolved L-Citrulline relative to the aqueous solution.
    Type: Grant
    Filed: May 14, 2012
    Date of Patent: January 21, 2014
    Assignee: Global Strategic Connections, LLC
    Inventors: Hua Bai, Peijie Yang, Zhengjie Chen, Chongyang Xu, Zhaorui Li, Zigang Zhao, Luyan Jiang, Zongyi Yang, Jiang Li
  • Patent number: 8623833
    Abstract: This invention provides compositions and methods for enhancing delivery of drugs and other agents across epithelial tissues, including the skin, gastrointestinal tract, pulmonary epithelium, ocular tissues and the like. The compositions and methods are also useful for delivery across endothelial tissues, including the blood brain barrier. The compositions and methods employ a delivery enhancing transporter that has sufficient guanidino or amidino sidechain moieties to enhance delivery of a compound conjugated to the reagent across one or more layers of the tissue, compared to the non-conjugated compound. The delivery-enhancing polymers include, for example, poly-arginine molecules that are preferably between about 6 and 25 residues in length.
    Type: Grant
    Filed: June 15, 2010
    Date of Patent: January 7, 2014
    Assignee: KAI Pharmaceuticals, Inc.
    Inventors: Jonathan B. Rothbard, Paul A. Wender, P. Leo McGrane, Lalitha V. S. Sista, Thorsten A. Kirschberg
  • Publication number: 20130344545
    Abstract: The present invention relates to a microorganism having an improved ornithine-producing ability, in which the biosynthetic pathway of arginine form ornithine is blocked, the intracellular glutamate level is increased, and the biosynthetic pathway of ornithine from glutamate is enhanced, and a method for producing ornithine using the microorganism.
    Type: Application
    Filed: December 8, 2011
    Publication date: December 26, 2013
    Applicant: CJ CHELJEDANG CORPORATION
    Inventors: Hyang Choi, Kyoung Min Lee, Min Sun Kang, Sung Hoo Jhon, Hye Won Um, Su Jin Choi, Han Won Lee, Soo An Shin
  • Publication number: 20130330787
    Abstract: A method produces a chemical through continuous fermentation including: (a) culturing a cell in a culture medium in a fermentor to ferment a feedstock to produce a chemical; (b) conducting filtration of the culture medium with a separation membrane module; (c) separating a permeate containing the chemical from the culture medium while retaining a non-permeated liquid in the fermentor, and (d) supplying a gas from at least one of a lower portion of the separation membrane module and a pipe communicating between the fermentor and the separation membrane module to adjust a gas linear velocity in the separation membrane module to 0.15 cm/s to 70 cm/s while supplying the separation membrane module with a liquid.
    Type: Application
    Filed: December 8, 2011
    Publication date: December 12, 2013
    Inventors: Satoko Kanamori, Jihoon Cheon, Takashi Mimitsuka, Norihiro Takeuchi, Makoto Nishida, Yuji Tanaka
  • Publication number: 20130323784
    Abstract: A target substance can be efficiently produced by culturing, in a medium, a coryneform bacterium in which the activity of a PTS protein relating to fructose uptake is reduced or lost as compared with a parent strain and the bacterium can produce the target substance, allowing the target substance to form and accumulate in a culture; and collecting the target substance from the culture
    Type: Application
    Filed: February 9, 2012
    Publication date: December 5, 2013
    Applicant: KYOWA HAKKO BIO CO., LTD.
    Inventors: Tetsuro Ujihara, Tetsuya Abe, Makoto Yagasaki
  • Publication number: 20130295621
    Abstract: A target substance can be produced by culturing a bacterium having an ability to produce 2-ketoglutaric acid or a derivative thereof, and an ability to produce xylonic acid from xylose, which is imparted with xylonate dehydratase activity, 2-keto-3-deoxyxylonate dehydratase activity and 2-ketoglutaric semialdehyde dehydrogenase activity, or in which these activities are enhanced, in a medium containing xylose as a carbon source to produce and accumulate the target substance in the medium, and collecting the target substance from the medium.
    Type: Application
    Filed: June 11, 2013
    Publication date: November 7, 2013
    Inventors: Yousuke Nishio, Youko Yamamoto, Kazuteru Yamada, Kosuke Yokota
  • Publication number: 20130288313
    Abstract: An L-amino acid is produced by culturing a microorganism belonging to the family Enterobacteriaceae having an L-amino acid-producing ability and modified so that glycerol dehydrogenase and dihydroxyacetone kinase activities are increased, in a medium containing glycerol as a carbon source to produce and accumulate an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells.
    Type: Application
    Filed: July 10, 2013
    Publication date: October 31, 2013
    Inventors: Yuri Nagai, Kazuyuki Hayashi, Takuji Ueda, Yoshihiro Usuda, Kazuhiko Matsui
  • Publication number: 20130224806
    Abstract: A method for producing an L-amino acid is described using a bacterium of the Enterobacteriaceae family, wherein the bacterium contains a protein which is able to confer resistance to growth inhibition by L-cysteine.
    Type: Application
    Filed: May 3, 2013
    Publication date: August 29, 2013
    Applicant: AJINOMOTO CO., INC.
    Inventor: AJINOMOTO CO., INC.
  • Publication number: 20130109063
    Abstract: The present invention provides a method for producing L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to enhance the expression of the bssR gene, which encodes a regulator of biofilm through signal secretion.
    Type: Application
    Filed: January 7, 2013
    Publication date: May 2, 2013
    Applicant: AJINOMOTO CO., INC.
    Inventor: AJINOMOTO CO., INC.
  • Publication number: 20130078682
    Abstract: The present invention provides a method for producing L-amino acids, such as L-arginine, L-citrulline, and L-lysine, using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to attenuate expression of one or more genes, such as the pepA, pepB, and pepD genes.
    Type: Application
    Filed: November 28, 2012
    Publication date: March 28, 2013
    Applicant: AJINOMOTO CO., INC.
    Inventor: AJINOMOTO CO., INC.
  • Publication number: 20130078681
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, in which expression of a gene encoding a lysine/arginine/ornithine transporter has been attenuated.
    Type: Application
    Filed: November 28, 2012
    Publication date: March 28, 2013
    Applicant: AJINOMOTO CO., INC.
    Inventor: AJINOMOTO CO., INC.
  • Patent number: 8404474
    Abstract: The present invention provides a bacterium which has an ability to produce a useful metabolite derived from acetyl-coenzyme A, such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, L-cysteine, succinate, and polyhydroxybutyrate, wherein said bacterium is modified so that activities of D-xylulose-5-phosphate phosphoketolase and/or fructose-6-phosphate phosphoketolase are enhanced. The present invention also provides a method for producing the useful metabolite using the bacterium.
    Type: Grant
    Filed: June 28, 2010
    Date of Patent: March 26, 2013
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yury Ivanovich Kozlov, Akito Chinen, Hiroshi Izui, Yoshihiko Hara, Hisashi Yasueda, Konstantin Vyacheslavovich Rybak, Ekaterina Aleksandrovna Slivinskaya, Joanna Yosifovna Katashkina
  • Patent number: 8383363
    Abstract: L-amino acids are produced by culturing a microorganism which has an ability to produce the L-amino acid, but has been modified so that expression of the ybjE gene has been enhanced. The L-amino acid is collected from the culture medium or from the microorganism.
    Type: Grant
    Filed: June 7, 2007
    Date of Patent: February 26, 2013
    Assignee: Ajinomoto Co., Inc.
    Inventors: Takuji Ueda, Yuta Nakai, Yoshiya Gunji, Rie Takikawa, Yuji Joe
  • Patent number: 8372606
    Abstract: The present invention provides a method for separating and obtaining a basic amino acid hydrochloride from a basic amino acid fermentation broth or an enzyme reaction solution which enzyme reaction is catalyzed by viable microbial cells which are able to produce a basic amino acid, each containing sulfate ions, wherein product yields and qualities are almost the same and are secured more easily, as compared with the conventional technique.
    Type: Grant
    Filed: June 25, 2009
    Date of Patent: February 12, 2013
    Assignee: Ajinomoto Co., Inc.
    Inventors: Mitsuhiro Kishino, Toshimichi Kamei
  • Publication number: 20130023016
    Abstract: The present invention relates to a polynucleotide that is active to an acetyl glutamate synthase and acetyl ornithinase which are associated with ornithine or arginine biosynthesis from Corynebacterium glutamicum. The present invention also relates to a polypeptide encoded by said polynucleotide, a recombinant vector comprising said polynucleotide, to a transformant obtained by introducing said recombinant vector to a host microorganism for producing L-ornithine or L-arginine, and transforming the recombinant vector, and to a method for producing L-ornithine or L-arginine by culturing said transformant. The activity of the transformant of the present invention to an acetyl glutamate synthase and acetyl ornithinase is increased as compared to an intrinsic activity, and thus L-ornithine or L-arginine can be produced, at a high yield rate, from the transformant of the present invention.
    Type: Application
    Filed: December 29, 2010
    Publication date: January 24, 2013
    Inventors: Jin-Man Cho, Hye-Won Kim, Ji-Hye Lee, Jae-Yong Cho
  • Publication number: 20130004999
    Abstract: The invention relates to an isolated polynucleotide having promoter activity, a variant of the promoter of the gap gene coding for glyceraldehyde-3-phosphate dehydrogenase; and to a microorganism which produces and/or secretes a fine chemical, the microorganism including the isolated polynucleotide having promoter activity, which enables various genes to be overexpressed in comparison with the particular starting strain; and to a process for preparing fine chemicals using the microorganism.
    Type: Application
    Filed: June 27, 2012
    Publication date: January 3, 2013
    Applicant: Evonik Degussa GmbH
    Inventors: Alexander RETH, Brigitte Bathe, Stephan Hans, Wilfried Claes
  • Publication number: 20120329105
    Abstract: The presently disclosed subject matter provides a bacterium of Enterobacteriaceae family producing L-aspartic acid or an L-aspartic acid-derived metabolite modified to have aspartate dehydrogenase and a method for producing L-aspartic acid or an L-aspartic acid-derived metabolite, such as L-threonine, L-lysine, L-arginine, L-methionine and L-homoserine, using such bacterium.
    Type: Application
    Filed: July 6, 2012
    Publication date: December 27, 2012
    Inventors: Tatyana Mikhailovna Kuvaeva, Sergey Vasilievich Smirnov, Olga Nikolaevna Ivanova, Aleksandr Dmitrievich Kivero, Joanna Yosifovna Katashkina
  • Publication number: 20120309061
    Abstract: Disclosed are processes for producing Arginine by fermentation in which sugars obtained by enzymatic starch hydrolysis from inexpensive starch containing agro-wastes, such as Cassava bagasse and Jackfruit seed powder, are fermented in the presence of microorganisms to produce a fermentated liquor containing Arginine, and recovering Arginine from the liquor. The process can economically be scaled up for the production of Arginine from unrefined sugar sources as it produced Arginine in higher yields, when compared to more expensive synthetic carbon sources, like dextrose or sucrose.
    Type: Application
    Filed: February 25, 2010
    Publication date: December 6, 2012
    Applicant: COLGATE-PALMOLIVE COMPANY
    Inventors: Ashok Pandey, K. Madhavan Nampoothiri, Ravi Subramanyam
  • Patent number: 8318465
    Abstract: A process for integrated utilization of the energy and material contents of hydrolysates and solids obtained in the enzymatic hydrolysis of renewable raw materials, in which the resulting hydrolysis solution is used as a carbon source in fermentations and the unhydrolysed solids are sent to biogas production.
    Type: Grant
    Filed: April 11, 2008
    Date of Patent: November 27, 2012
    Assignee: Evonik Degussa GmbH
    Inventors: Murillo Villela Filho, Isabelle Schaarschmidt, Bernd Wahl, Elmar Rother, Hartmut Zimmermann, Andreas Karau
  • Publication number: 20120296116
    Abstract: Processes for producing a suitable purity grade of L-Citrulline are disclosed. The processes can include contacting crude L-Citrulline in an aqueous solution with an adsorptive medium at a temperature above approximately 50° C. and below the temperature of denaturement for the L-Citrulline for an interval sufficient to remove at least one contaminant from the L-Citrulline. The processes can also include concentrating the dissolved L-Citrulline relative to the aqueous solution.
    Type: Application
    Filed: May 14, 2012
    Publication date: November 22, 2012
    Applicant: GLOBAL STRATEGIC CONNECTIONS, LLC
    Inventors: Hua Bai, Peijie Yang, Zhengjie Chen, Chongyang Xu, Zhaorui Li, Zigang Zhao, Luyan Jiang, Zongyi Yang, Jiang Li
  • Patent number: 8313933
    Abstract: An L-amino acid can be produced by culturing an L-amino acid-producing bacterium which belongs to the Enterobacteriaceae family and which has been modified so that the expression of a yggG gene is enhanced.
    Type: Grant
    Filed: August 25, 2009
    Date of Patent: November 20, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Masahito Taya, Motomu Nishioka, Yoshihiro Ojima, Mizuho Komaki, Shintaro Iwatani
  • Patent number: 8309320
    Abstract: The invention features methods and compositions for diagnosis, including prognosis, of conditions associated with decreased arginine bioavailability (which can result from dysregulated arginine metabolism, e.g., due to increased arginase activity) by assessing in a sample from a subject the ratio of arginine to one or more, usually two or more, modulators of arginine bioavailability. In one embodiment, the ratio of arginine to (ornithine+citrulline) is assessed to aid in diagnosis.
    Type: Grant
    Filed: December 1, 2009
    Date of Patent: November 13, 2012
    Assignees: Children's Hospital & Research Center at Oakland, The Cleveland Clinic Foundation
    Inventors: Claudia R. Morris, Stanley L. Hazen
  • Publication number: 20120237985
    Abstract: A method for producing an L-amino acid by culturing a coryneform bacterium having an L-amino acid-producing ability in a medium to produce and accumulate the L-amino acid in the medium or cells of the bacterium, and collecting the L-amino acid from the medium or cells, wherein said coryneform bacterium has been modified to enhance carbonic anhydrase activity.
    Type: Application
    Filed: February 24, 2012
    Publication date: September 20, 2012
    Inventors: Takeshi Nagahiko, Jun Nakamura
  • Publication number: 20120149072
    Abstract: A method for producing a basic substance by fermentation comprising culturing a microorganism having an ability to produce the basic substance in a liquid medium contained in a fermentation tank to produce and accumulate the basic substance in the medium, wherein amount of sulfate and/or chloride ions used as counter ions of the basic substance is reduced by adjusting total ammonia concentration in the medium to be within a specific concentration range during at least a part of the total period of culture process.
    Type: Application
    Filed: February 24, 2012
    Publication date: June 14, 2012
    Inventors: Ryo Takeshita, Shinichi Sugimoto
  • Patent number: 8198053
    Abstract: A method for producing a basic substance by fermentation comprising culturing a microorganism having an ability to produce the basic substance in a liquid medium contained in a fermentation tank to produce and accumulate the basic substance in the medium, wherein amount of sulfate and/or chloride ions used as counter ions of the basic substance is reduced by adjusting total ammonia concentration in the medium to be within a specific concentration range during at least a part of the total period of culture process.
    Type: Grant
    Filed: July 12, 2010
    Date of Patent: June 12, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Ryo Takeshita, Shinichi Sugimoto
  • Patent number: 8192963
    Abstract: A bacterium is described which belongs to the Enterobacteriaceae family, and has an ability to produce an L-amino acid, such as L-glutamic acid, L-arginine and L-threonine. The bacterium is modified so that the activity of a protein encoded by ydcI gene is decreased, thereby producing and accumulating the L-amino acid selected from L-glutamic acid, L-arginine, and L-threonine in the culture medium or cells of the bacterium when cultured in a culture medium. Subsequently, the L-amino acid is collected from the culture medium or the bacterium.
    Type: Grant
    Filed: March 1, 2011
    Date of Patent: June 5, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yousuke Nishio, Yoshihiro Usuda
  • Patent number: 8187850
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the ybiV gene.
    Type: Grant
    Filed: January 30, 2008
    Date of Patent: May 29, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Konstantin Vyacheslavovich Rybak, Marina Evgenievna Sheremet'eva, Aleksandra Yurievna Skorokhodova, Tatyana Viktorovna Leonova, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov, legal representative
  • Patent number: 8114639
    Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the sfmACDFH-fimZ cluster and/or the fimZ gene.
    Type: Grant
    Filed: October 17, 2008
    Date of Patent: February 14, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Dmitriy Vladimirovich Filippov, Vera Georgievna Doroshenko, Aleksandra Yurievna Skorokhodova, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner