Alanine; Leucine; Isoleucine; Serine; Homoserine Patents (Class 435/116)
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Publication number: 20120329095Abstract: Provided is a method of producing succinic acid in which a microorganism having a succinic acid-producing ability is allowed to react with a sugar, the method being characterized in that the ratio of the oxygen transfer rate to the succinic acid production rate (mmol-O2/mol-SA) is 0.1 to 240 and that the doubling time of the microorganism during the reaction is not shorter than 40 hours.Type: ApplicationFiled: September 7, 2012Publication date: December 27, 2012Applicant: MITSUBISHI CHEMICAL CORPORATIONInventors: Katsunori YOSHIKAWA, Daisuke KIDO, Makoto MURASE
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Publication number: 20120329107Abstract: The present invention relates to a method for producing D- or L-tert-leucine or D- or L-tert-leucine amide by reacting DL-tert-leucine amide with a biocatalyst selected from the group consisting of an enzyme capable of hydrolyzing the DL-tert-leucine amide stereoselectively, cells of a microorganism having the enzyme, a material produced by the treatment of the cells, an immobilized enzyme produced by immobilizing the enzyme onto a carrier, immobilized cells produced by immobilizing the cells onto a carrier, and an immobilized cell treatment product obtained by immobilizing the cell treatment product onto a carrier to thereby hydrolyze the DL-tert-leucine amide, wherein the hydrolysis is carried out with separating ammonia produced by the hydrolysis from the hydrolysis reaction solution.Type: ApplicationFiled: December 3, 2010Publication date: December 27, 2012Applicant: Mitsubishi Gas Chemical Company, Inc.Inventors: Shin Iida, Sachiko Arie, Yusuke Tanaka
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Patent number: 8318465Abstract: A process for integrated utilization of the energy and material contents of hydrolysates and solids obtained in the enzymatic hydrolysis of renewable raw materials, in which the resulting hydrolysis solution is used as a carbon source in fermentations and the unhydrolysed solids are sent to biogas production.Type: GrantFiled: April 11, 2008Date of Patent: November 27, 2012Assignee: Evonik Degussa GmbHInventors: Murillo Villela Filho, Isabelle Schaarschmidt, Bernd Wahl, Elmar Rother, Hartmut Zimmermann, Andreas Karau
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Patent number: 8313933Abstract: An L-amino acid can be produced by culturing an L-amino acid-producing bacterium which belongs to the Enterobacteriaceae family and which has been modified so that the expression of a yggG gene is enhanced.Type: GrantFiled: August 25, 2009Date of Patent: November 20, 2012Assignee: Ajinomoto Co., Inc.Inventors: Masahito Taya, Motomu Nishioka, Yoshihiro Ojima, Mizuho Komaki, Shintaro Iwatani
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Publication number: 20120252075Abstract: The invention relates to a microorganism which produces and/or secretes an organic-chemical compound, wherein the microorganism has increased expression, compared to the particular starting strain, of one or more protein subunits of the ABC transporter having the activity of a trehalose importer, said microorganism being capable of taking up trehalose from the medium; and to a method for the production of an organic-chemical compound, using the microorganism according to the invention, wherein accumulation of trehalose in the fermentation broth is reduced or avoided.Type: ApplicationFiled: April 3, 2012Publication date: October 4, 2012Applicant: Evonik Degussa GmbHInventors: Stephan Hans, Brigitte Bathe, Alexander Reth, Wilfried Claes, Reinhard Krämer, Gerd Seibold, Alexander Henrich
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Patent number: 8278076Abstract: Microbial production of pyruvate and metabolites derived from pyruvate in cells exhibiting reduced pyruvate dehydrogenase activity compared to wild-type cells. Acetate and glucose are supplied as a carbon sources.Type: GrantFiled: June 28, 2010Date of Patent: October 2, 2012Assignee: University of Georgia Research Foundation, Inc.Inventors: Mark A. Eiteman, Elliot Altman
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Patent number: 8278075Abstract: L-Cysteine, L-cystine, a derivative or precursor thereof, or a mixture thereof is produced by culturing a bacterium belonging to the family Enterobacteriaceae, which has L-cysteine-producing ability and has been modified so that the activity of a protein encoded by a tolC gene, for example, a protein defined in the following (a) or (b), is increased in a medium, and by collecting L-cysteine, L-cystine, a derivative or precursor thereof, or a mixture thereof from the medium: (a) a protein comprising the amino acid sequence of SEQ ID NO: 2, (b) a protein comprising the amino acid sequence of SEQ ID NO: 2, but wherein one or several amino acid residues are substituted, deleted, inserted or added, increase of which activity in the bacterium improves the ability of the bacterium to produce L-cysteine.Type: GrantFiled: August 18, 2010Date of Patent: October 2, 2012Assignees: Ajinomoto Co., Inc., National University Corporation Nara Institute of Science and TechnologyInventors: Gen Nonaka, Hiroshi Takagi, Iwao Ohtsu
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Publication number: 20120237979Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: September 30, 2010Publication date: September 20, 2012Applicant: NOVOZYMES A/SInventors: Junxin Duan, Kirk Matthew Schnorr, Wenping Wu
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Publication number: 20120237985Abstract: A method for producing an L-amino acid by culturing a coryneform bacterium having an L-amino acid-producing ability in a medium to produce and accumulate the L-amino acid in the medium or cells of the bacterium, and collecting the L-amino acid from the medium or cells, wherein said coryneform bacterium has been modified to enhance carbonic anhydrase activity.Type: ApplicationFiled: February 24, 2012Publication date: September 20, 2012Inventors: Takeshi Nagahiko, Jun Nakamura
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Publication number: 20120219997Abstract: The present invention refers to the new Escherichia coli strains denominated JU15, JU15A, LL26 and MS04 and their derivatives that produce metabolites, particularly D-lactate, L-lactate or ethanol, with high yield and selectivity from a wide variety of carbon sources, such as culture media with a high xylose content (as the main carbon source) and, in particular, media formulated with hydrolyzed vegetables, such as sugarcane bagasse, agave bagasse and fast-growing grasses, and a wide variety of agricultural and industrial wastes, such as whey or forestry wastes, celluloses, grasses, agave bagasse, paper wastes, shavings and sawdust, shrubs and generally any material derived from lignocellulose. These strains use the production of the metabolite of interest (especially D-lactate, L-lactate or ethanol) as the only way of regenerating the reducing power.Type: ApplicationFiled: August 6, 2010Publication date: August 30, 2012Inventors: Alfredo Martinez Jimenez, Guillermo Gosset Lagarda, Georgina Teresa Hernandez Chavez, Gerardo Huerta Beristain, Berenice Trujillo Martinez, Jose Utrilla Carreri
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Publication number: 20120214211Abstract: The invention relates to a recombinant coryneform bacterium which secretes an organic chemical compound and in which the sugR gene which codes for a polypeptide having the activity of an SugR regulator has been attenuated. The invention further relates to a processes for using this bacterium for the fermentative preparation of organic chemical compounds.Type: ApplicationFiled: January 21, 2012Publication date: August 23, 2012Applicants: Forschungszentrum Julich GmbH, Evonik Degussa GmbHInventors: Brigitte Bathe, Bastian Blombach, Bernhard Eikmanns, Verena Engels, Georg Thierbach, Volker Wendisch
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Publication number: 20120190079Abstract: The present invention relates to a method for racemizing an optically active ?-amino acid with a viable bacterial cell producing ?-aminobutyric acid aminotransferase or a processed product thereof. According to the present invention, racemic ?-amino acids of high quality can be manufactured inexpensively.Type: ApplicationFiled: June 24, 2010Publication date: July 26, 2012Applicant: MITSUBISHI GAS CHEMICAL COMPANY, INC.Inventors: Atsushi Inoue, Yusuke Tanaka
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Publication number: 20120164698Abstract: The invention relates to a method for preparing organic-chemical compounds, characterized in that the following steps are carried out: a) fermentation of a microorganism secreting an L-amino acid, which microorganism contains an overexpressed polynucleotide coding for a polypeptide having polyphosphate-dependent NAD+ kinase activity, in a fermentation medium, to form a fermentation broth, b) accumulation of said compound in said fermentation broth and/or in the cells of said microorganism. The invention relates to a method for preparing organic-chemical compounds by fermentation of a microorganism in which a polypeptide having polyphosphate-dependent NAD+ kinase is overexpressed.Type: ApplicationFiled: June 18, 2010Publication date: June 28, 2012Applicant: EVONIK DEGUSSA GMBHInventors: Volker F. Wendisch, Steffen Lindner, Brigitte Bathe, Wilfried Claes
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Publication number: 20120156735Abstract: The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.Type: ApplicationFiled: June 15, 2011Publication date: June 21, 2012Applicant: BUTAMAX(TM) ADVANCED BIOFUELS LLCInventors: Michael Dauner, Lori Ann Maggio-Hall, Jean-Francois Tomb
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Publication number: 20120142778Abstract: Method for the microbiological production of cinnamoyl amide derivatives of amino acids, certain products that result therefrom and uses thereof, especially as antioxidants.Type: ApplicationFiled: June 5, 2009Publication date: June 7, 2012Inventor: Jean Lorquin
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Patent number: 8187850Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the ybiV gene.Type: GrantFiled: January 30, 2008Date of Patent: May 29, 2012Assignee: Ajinomoto Co., Inc.Inventors: Konstantin Vyacheslavovich Rybak, Marina Evgenievna Sheremet'eva, Aleksandra Yurievna Skorokhodova, Tatyana Viktorovna Leonova, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov, legal representative
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Patent number: 8178322Abstract: A method is described for producing an L-amino acid or a nucleic acid by culturing a microorganism having an ability to produce the L-amino acid or nucleic acid in a liquid medium in a fermentation tank containing a stirring impeller, and optionally adding seed crystals to the medium as required to produce and accumulate crystals of the L-amino acid or nucleic acid in the medium, and collecting crystals of the L-amino acid or nucleic acid from the culture. The power density of the stirring impeller is controlled to be 2.4 kW/m3 or lower after either precipitation of the crystals or addition of the seed crystals.Type: GrantFiled: August 14, 2009Date of Patent: May 15, 2012Assignee: Ajinomoto Co., Inc.Inventors: Masayuki Araki, Yusuke Takahashi, Akihiro Watanabe, Fumito Ohnishi, Takahiro Asano, Kazuya Kondo, Wataru Hibino, Shintaro Iwatani, Satoshi Okutani
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Publication number: 20120114655Abstract: The subject invention provides at least one nucleic acid sequence encoding an aldouronate-utilization regulon isolated from Paenibacillus sp. strain JDR-2, a bacterium which efficiently utilizes xylan and metabolizes aldouronates (methylglucuronoxylosaccharides). The subject invention also provides a means for providing a coordinately regulated process in which xylan depolymerization and product assimilation are coupled in Paenibacillus sp. strain JDR-2 to provide a favorable system for the conversion of lignocellulosic biomass to biobased products. Additionally, the nucleic acid sequences encoding the aldouronate-utilization regulon can be used to transform other bacteria to form organisms capable of producing a desired product (e.g., ethanol, 1-butanol, acetoin, 2,3-butanediol, 1,3-propanediol, succinate, lactate, acetate, malate or alanine) from lignocellulosic biomass.Type: ApplicationFiled: January 16, 2012Publication date: May 10, 2012Applicant: University of Florida Research Foundation, Inc.Inventors: JAMES F. PRESTON, III, Virginia Chow, Guang Nong, John D. Rice, Franz J. St. John
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Publication number: 20120107883Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.Type: ApplicationFiled: January 5, 2012Publication date: May 3, 2012Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov, Sakayu Shimizu, Jun Ogawa, Makoto Hibi
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Publication number: 20120107884Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.Type: ApplicationFiled: January 5, 2012Publication date: May 3, 2012Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov, Sakayu Shimizu, Jun Ogawa, Makoto Hibi
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Publication number: 20120077233Abstract: This invention relates to DNA encoding a novel enzyme having activity of synthesizing D-serine from formaldehyde and glycine, recombinant DNA constructed by integrating such DNA into a vector, a transformant transformed with the recombinant DNA, and a method for producing D-serine from formaldehyde and glycine with the use of the enzyme.Type: ApplicationFiled: February 24, 2011Publication date: March 29, 2012Applicant: Mitsui Chemicals, Inc.Inventors: Tadashi Araki, Tomonori Hidesaki, Seiichi Watanabe, Keita Nishida, Kiyoteru Nagahara, Mitsuo Koito
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Patent number: 8133714Abstract: The invention relates to a recombinant coryneform bacterium which secretes an organic chemical compound and in which the sugR gene which codes for a polypeptide having the activity of an SugR regulator has been attenuated. The invention further relates to a processes for using this bacterium for the fermentative preparation of organic chemical compounds.Type: GrantFiled: September 19, 2008Date of Patent: March 13, 2012Assignees: Evonik Degussa GmbH, Forschungszentrum JĂĽlich GmbHInventors: Brigitte Bathe, Bastian Blombach, Bernhard Eikmanns, Verena Engels, Georg Thierbach, Volker Wendisch
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Patent number: 8119374Abstract: This invention relates to the nucleotide sequence of coryneform bacteria coding for proteins which are involved in L-serine metabolism with reduced and switched off L-serine dehydratase activity. The invention also relates to microorganisms used in methods for producing L-serine.Type: GrantFiled: June 10, 2008Date of Patent: February 21, 2012Assignee: Forschungszentrum Julich GmbHInventors: Petra Peters-Wendisch, Roman Netzer, Lothar Eggeling, Hermann Sahm
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Patent number: 8114639Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus Escherichia or Pantoea, which has been modified to attenuate expression of the sfmACDFH-fimZ cluster and/or the fimZ gene.Type: GrantFiled: October 17, 2008Date of Patent: February 14, 2012Assignee: Ajinomoto Co., Inc.Inventors: Dmitriy Vladimirovich Filippov, Vera Georgievna Doroshenko, Aleksandra Yurievna Skorokhodova, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
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Publication number: 20120015409Abstract: The present invention provides a method of improving efficiency of a fermentative production of an L-amino acid.Type: ApplicationFiled: February 9, 2010Publication date: January 19, 2012Applicant: KYOWA HAKKO BIO CO., LTD.Inventors: Kazuhiko Tabata, Akihiro Senoo
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Patent number: 8076106Abstract: A process for producing high yields of enantioselective amino acids and chiral amines by reacting a keto acid or ketone and an amino acid donor in the presence of a transaminase biocatalyst to produce a keto acid by-product and an amino acid or amine product. Further reacting the keto acid by-product with a peroxide to increase the yield of additional amino acid or amine product.Type: GrantFiled: August 6, 2009Date of Patent: December 13, 2011Assignee: Richmond Chemical CorporationInventors: Ian Fotheringham, Nicholas Oswald
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Publication number: 20110281311Abstract: A bacterium which belongs to the family Enterobacteriaceae, and has an ability to produce L-lysine, L-threonine, L-asparagine, L-aspartic acid, L-methionine, L-alanine, L-isoleucine, and/or L-homoserine. The bacterium has been modified so that expression of the gltP and/or gltS genes is/are increased when cultured in a medium, resulting in the accumulation of the L-amino acid(s) in the medium or bacterial cells.Type: ApplicationFiled: May 24, 2011Publication date: November 17, 2011Inventors: Yuori Wakasa, Ryo Takeshita
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Publication number: 20110269185Abstract: The present invention provides compositions and methods designed to increase value output of a fermentation reaction that yields a first product, intended for commercialization, such as ethanol, and a fermentation residual used, for example, as animal feed. The methods involve using microorganisms in the fermentation process that have been modified so as to yield a residual having greater value that a residual produced in the process by a microorganism not so modified. In particular, the present invention contemplates using microorganisms in a fermentation process that have been modified to increase production of a nutrient, such as an essential amino acid, thereby reducing the need to supplement the nutrient in the animal's diet. The present invention also provides a modified fermentation residual of higher commercial value. Also provided in the present invention are complete animal feeds, nutritional supplements comprising the subject ferment residuals.Type: ApplicationFiled: April 27, 2011Publication date: November 3, 2011Inventor: Peter R. David
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Patent number: 8048651Abstract: The present invention features methods of increasing the production of a fine chemical, e.g., lysine from a microorganism, e.g., Corynebacterium by way of deregulating an enzyme encoding gene, i.e., fructose-1,6-bisphosphatase. In a preferred embodiment, the invention provides methods of increasing the production of lysine in Corynebacterium glutamicum by way of increasing the expression of fructose-1,6-bisphosphatase activity. The invention also provides a novel process for the production of lysine by way of regulating carbon flux towards oxaloacetate (OAA). In a preferred embodiment, the invention provides methods for the production of lysine by way of utilizing fructose or sucrose as a carbon source.Type: GrantFiled: July 31, 2009Date of Patent: November 1, 2011Assignee: BASF SEInventors: Oskar Zelder, Corinna Klopprogge, Hartwig Schroder, Stefan Haefner, Burkhard Kröger, Patrick Kiefer, Elmar Heinzle, Christoph Wittmann
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Publication number: 20110250654Abstract: Glycerol or other reduced carbon sources may be used as a feedstock for the microbial production of chemical products under certain microaerobic conditions. For example, such production may occur under microaerobic or microrespiratory conditions in which electron acceptors are consumed in the reaction as quickly as they are added. In such reactions, the reaction product is at least as reduced as carbon source. Further, during such a reaction, at least some of the carbon source is used to generate cell mass. In addition, microorganisms with modified genomes are provided for carrying out the methods herein.Type: ApplicationFiled: October 28, 2009Publication date: October 13, 2011Applicants: Glycos Biotechnologies, Inc., William Marsh Rice UniversityInventors: Ramon Gonzalez, Paul Campbell
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Publication number: 20110244530Abstract: The present invention provides an L-aminoacylase which is able to produce L-tert-leucine being useful as an intermediate for pharmaceuticals. A protein which is characterized in being represented by any of the following (a) to (d): (a) a protein coded by a gene consisting of a nucleic acid sequence shown in SEQ ID No: 1; (b) a protein consisting of an amino acid sequence shown in SEQ ID No: 2; (c) a protein coded by a polynucleotide which hybridizes under a stringent condition with a nucleic acid sequence which is complementary to the nucleic acid sequence shown in SEQ ID No: 1 and having an L-succinylaminoacylase activity; and (d) a protein which consists of an amino acid sequence where one or several amino acid (s) is/are substituted, deleted, inserted and/or added in the protein consisting of the amino acid sequence shown in SEQ ID No: 2 and has an L-succinylaminoacylase activity.Type: ApplicationFiled: May 7, 2009Publication date: October 6, 2011Applicants: TOYO BOSEKI KABUSHIKI KAISHA, SEKISUI MEDICAL CO., LTD.Inventors: Atsushi Toda, Sachio Iwai, Yoshiaki Nishiya, Takeshi Miyata, Aya Oosato, Shinya Kumagai, Toshihide Yamada
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Patent number: 8030051Abstract: The present disclosure relates generally to polypeptides having improved alanine 2,3-aminomutase (AAM) activity, the polynucleotides encoding the AAM polypeptides, and expression vectors and host cells for expressing the AAM polypeptides.Type: GrantFiled: October 16, 2006Date of Patent: October 4, 2011Assignee: Codexis, Inc.Inventors: Svetlana Balatskaya, Birthe Borup, Ranjini Chatterjee, Ish Dhawan, Richard J. Fox, Kenneth W. Mitchell, Emily C. Mundorff, Les Partridge, Matthew Tobin
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Publication number: 20110223284Abstract: The present invention provides compositions and methods designed to increase value output of a fermentation reaction. In particular, the present invention provides a business method of increasing value output of a fermentation plant. The present invention also provides a modified fermentation residual of higher commercial value. Also provided in the present invention are complete animal feeds, nutritional supplements comprising the subject ferment residuals. Further provided by the present invention is a method of performing fermentation, a modified fermentative microorganism and a genetic vehicle for modifying such microorganism.Type: ApplicationFiled: October 14, 2010Publication date: September 15, 2011Applicant: Ambrozea, Inc.Inventor: Peter R. David
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Patent number: 8012722Abstract: L-amino acids such as L-glutamic acid, L-glutamine, L-proline, L-arginine, L-leucine, and L-cysteine are produced by culturing in a medium a bacterium having an L-amino acid-producing ability and wherein the bacterium has been modified so that the phosphotransacetylase activity is enhanced.Type: GrantFiled: July 25, 2008Date of Patent: September 6, 2011Assignee: Ajinomoto Co., Inc.Inventors: Akito Chinen, Hisashi Yasueda, Jun Nakamura
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Publication number: 20110212496Abstract: An L-amino acid is produced by culturing a bacterium belonging to the family Enterobacteriaceae, which has an L-amino acid-producing ability and inherently has a native activity of a glucose dehydrogenase that uses pyrroloquinoline quinone as a coenzyme, but has been modified so that the activity of the glucose dehydrogenase is reduced, in a medium, and collecting the L-amino acid from the medium.Type: ApplicationFiled: March 1, 2011Publication date: September 1, 2011Inventors: Rie Takikawa, Yoshihiko Hara, Gen Nonaka, Kazuhiro Takumi
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Patent number: 8003771Abstract: The present invention relates to compositions useful as probes and in other applications and methods of their use. In some embodiments, nucleotides are prepared and functionalized with dyes. In some embodiments a first molecule is functionalized with an alkynyl group, a second molecule is functionalized with an azide group, and said first and second molecules are mixed under conditions to form a conjugate with a 1,2,3-triazol group. In further embodiments, a nucleotide is functionalized with an alkynyl group, a dye is functionalized with an azide group, and mixing the nucleotide and the dye forms a conjugate capable of emitting light.Type: GrantFiled: March 1, 2010Date of Patent: August 23, 2011Assignee: Third Wave Technologies, Inc.Inventors: Zbigniew Skrzypczynski, Sarah R Wayland
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Patent number: 7985562Abstract: The present invention is directed to a method identifying a risk for a thrombogenic disorder, to a method for selecting patients with a risk for a thrombogenic disorder, to a method for identifying a pharmaceutical for the therapy or prophylaxis of a thrombogenic disorder as well as to a method for producing a medicament and a diagnostic by employing the TAFI-Ile347 polymorphism.Type: GrantFiled: May 29, 2009Date of Patent: July 26, 2011Assignee: Sanofi-Aventis Deutsckland GmbHInventors: Detlef Kozian, Stefan Schaefer, Bernward Schoelkens, Karl-Ernst Siegler, Jean-Francois Deleuze, Sylvain Ricard, Sandrine Mace
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Publication number: 20110177566Abstract: A method for producing an L-amino acid is described using a bacterium of the Enterobacteriaceae family, wherein the bacterium contains a protein which is able to confer resistance to growth inhibition by L-cysteine.Type: ApplicationFiled: January 11, 2011Publication date: July 21, 2011Inventors: Ekaterina Alekseevna Savrasova, Natalia Viktorovna Stoynova, Gen Nonaka, Shunsuke Yamazaki, Kazuhiro Takumi
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Publication number: 20110171695Abstract: An isolated mutant of coryneform bacteria comprising a gene encodes a polypeptide having 2-methylcitrate dehydratase activity, where the polypeptide comprises an amino acid sequence in which one of the proteinogenic amino acids except L-proline is present at position 272 or a corresponding or comparable position. In addition, an isolated polynucleotide encoding a polypeptide having 2-methylcitrate dehydratase enzymic activity, which comprises at position 272 of the amino acid sequence or a corresponding or comparable position a proteinogenic amino acid except L-proline is described. A method for producing a recombinant coryneform bacterium and L-amino acids. A recombinant microorganism, L-Lysine-containing feed additive, and L-Tryptophan-containing feed additive is also described.Type: ApplicationFiled: January 19, 2011Publication date: July 14, 2011Applicant: EVONIK DEGUSSA GmbHInventors: Brigitte BATHE, Caroline Kreutzer, Georg Thierbach
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Publication number: 20110124060Abstract: Crabtree positive yeast cells that have endogenous expressed pyruvate decarboxylase genes inactivated and an engineered biosynthetic pathway utilizing pyruvate were found to have improved growth and product yield when glucose repression was reduced. These cells were able to grow in media containing a high glucose concentration.Type: ApplicationFiled: September 29, 2010Publication date: May 26, 2011Applicant: BUTAMAX(TM) ADVANCED BIOFUELS LLCInventors: LARRY CAMERON ANTHONY, Mark J. Nelson
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Publication number: 20110124061Abstract: A method for the preparation of the polyunsaturated fatty acids-containing phosphatidylserine, the method comprising: combining L-serine with a fish liver phosphatidylcholine having a polyunsaturated fatty acid to form a mixture; reacting the mixture with phospholipase D to effect transphosphatidylation of L-serine and the phosphatidylcholine having polyunsaturated fatty acids to produce the polyunsaturated fatty acids-containing phosphatidylserine.Type: ApplicationFiled: January 27, 2011Publication date: May 26, 2011Inventors: Su Chen, Hung Kwong
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Publication number: 20110111466Abstract: The present invention relates to a transformed microorganism producing an L-amino acid using sucrose as a main carbon source, and a method for producing an L-amino acid using the same.Type: ApplicationFiled: February 11, 2010Publication date: May 12, 2011Applicant: CJ Cheiljedang CorporationInventors: Jae Yeong Ju, Kwang Ho Lee, Hyun Ae Bae, Hyo Jin Kim, Keun Chul Lee, Young Bin Hwang, Kyu Soo Cho, Hye Min Park, Hyun Ah Kim
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Patent number: 7919285Abstract: This invention relates to DNA encoding an enzyme having activity of synthesizing D-serine from formaldehyde and glycine, recombinant DNA constructed by integrating such DNA into a vector, a transformant transformed with the recombinant DNA, and a method for producing D-serine from formaldehyde and glycine with the use of the enzyme.Type: GrantFiled: October 7, 2005Date of Patent: April 5, 2011Assignee: Mitsui Chemicals, Inc.Inventors: Tadashi Araki, Tomonori Hidesaki, Seiichi Watanabe, Keita Nishida, Kiyoteru Nagahara, Mitsuo Koito
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Publication number: 20110053227Abstract: The invention relates to a process for preparing L-amino acids by fermenting recombinant microorganisms of the Enterobacteriaceae family, characterized in that a) the desired L-amino acid-producing microorganisms, in which the yjcG-ORF, or nucleotide sequences or alleles encoding the gene product, is/are enhanced, in particular overexpressed, is cultured in a medium under conditions under which the desired L-amino acid is accumulated in the medium or in the cells, and b) the desired L-amino acid is isolated, with, where appropriate, constituents of the fermentation broth, and/or the biomass remaining in its/their entirety or in portions (from ?0 to 100%) in the isolated product or being removed completely.Type: ApplicationFiled: January 22, 2010Publication date: March 3, 2011Inventor: Nicole DUSCH
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Patent number: 7888078Abstract: The present invention provides: a process for producing an amino acid which comprises adding crystals of the amino acid having an average particle size of 1 to 120 ?m to a medium so that the concentration of the crystals of the amino acid becomes 0.5 g/l or more, culturing a microorganism having the ability to produce the amino acid in the medium, allowing crystals of the amino acid to form and accumulate in the medium, and recovering the crystals of the amino acid from the culture; and a process for producing an amino acid which comprises adding crystals of the amino acid to a medium so that the total surface area of the crystals of the amino acid in the medium becomes 0.02 m2/l, culturing a microorganism having the ability to produce the amino acid in the medium, allowing crystals of the amino acid to form and accumulate in the medium, and recovering the crystals of the amino acid from the culture.Type: GrantFiled: April 12, 2006Date of Patent: February 15, 2011Assignee: Kyowa Hakko Bio Co., Ltd.Inventors: Tsuyoshi Shimose, Ryou Ohashi, Katsumi Fujinaga
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Patent number: 7888077Abstract: The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus Escherichia or Pantoea, which has been modified to attenuate expression of the kefB gene.Type: GrantFiled: November 5, 2007Date of Patent: February 15, 2011Assignee: Ajinomoto., Inc.Inventors: Dmitriy Vladimirovich Filippov, Elvira Borisovna Voroshilova, Mikhail Markovich Gusyatiner
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Publication number: 20110033902Abstract: L-Cysteine, L-cystine, a derivative or precursor thereof, or a mixture thereof is produced by culturing a bacterium belonging to the family Enterobacteriaceae, which has L-cysteine-producing ability and has been modified so that the activity of a protein encoded by a tolC gene, for example, a protein defined in the following (a) or (b), is increased in a medium, and by collecting L-cysteine, L-cystine, a derivative or precursor thereof, or a mixture thereof from the medium: (a) a protein comprising the amino acid sequence of SEQ ID NO: 2, (b) a protein comprising the amino acid sequence of SEQ ID NO: 2, but wherein one or several amino acid residues are substituted, deleted, inserted or added, increase of which activity in the bacterium improves the ability of the bacterium to produce L-cysteine.Type: ApplicationFiled: August 18, 2010Publication date: February 10, 2011Inventors: Gen Nonaka, Hiroshi Takagi, Iwao Ohtsu
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Publication number: 20100330623Abstract: The present invention relates generally to compositions and methods useful for, inter alia, production of commercial biologic products such as amino acids. More specifically, the present invention relates to genetically modified strains of microorganisms and the use thereof for the production of commercial products. The present invention also provides, inter alia, novel isolated DNA, nucleic acid, vectors and reduced genome bacteria.Type: ApplicationFiled: February 19, 2009Publication date: December 30, 2010Applicant: SCARAB GENOMICS LLCInventors: Frederick R. Blattner, Sun Chang Kim, Jun Hyoung Lee
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Publication number: 20100323409Abstract: A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof using an L-isoleucine-producing bacterium transformed with a DNA fragment containing a gene coding for a protein having L-isoleucine dioxygenase activity; and having the ability to produce (2S,3R,4S)-4-hydroxy-L-isoleucine.Type: ApplicationFiled: June 21, 2010Publication date: December 23, 2010Inventors: Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Olga Sergeevna Beznoschenko
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Patent number: RE43764Abstract: The present invention relates to a purifying process for phosphatidylserine, the latter being prepared by trans-phosphatidylation of phosphatidylcholine with serine in presence of the enzyme D-phospholipase and containing as impurities hydrophilic compounds, proteins and inorganic salts.Type: GrantFiled: June 24, 2011Date of Patent: October 23, 2012Assignee: Chemi S.p.A.Inventors: Lorenzo De Ferra, Pietro Massardo