Nitrogen As Only Ring Hetero Atom Patents (Class 435/121)
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Patent number: 5242815Abstract: A method for producing streptovaricin by culturing a streptovaricin producing strain in the presence of a nonionic adsorbent. This procedure substantially increases the production efficiency of the streptovaricin. A second embodiment wherein the streptovaricin producing strain is produced in the presence of fumaric acid or its water soluble salts to provide improvements in production efficiency is also disclosed.Type: GrantFiled: April 29, 1992Date of Patent: September 7, 1993Assignees: Shin-Etsu Chemical Co., Ltd., The Institute of Physical Chemical ResearchInventors: Isao Endo, Shigehiro Nagura, Kaname Inoue, Jun Watanabe
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Patent number: 5236832Abstract: A microbiological process for the oxidation of methyl groups in aromatic 5- or 6-member ring heterocycles to the corresponding carboxylic acid. The heterocycle serves as the substrate and exhibits no substituent on the carbon atoms adjacent to the methyl groups to be oxidized. The reaction of the heterocycle takes place by means of microorganisms of the genus Pseudomonas utilizing toluene, xylene, or cymene. The enzymes of the microorganisms been previously induced.Type: GrantFiled: October 30, 1991Date of Patent: August 17, 1993Assignee: Lonza, Ltd.Inventor: Andreas Kiener
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Patent number: 5233050Abstract: An alkyl indole of the formula: ##STR1## is a useful antimigraine agent.Type: GrantFiled: February 7, 1992Date of Patent: August 3, 1993Assignee: Merck & Co., Inc.Inventors: Robert P. Borris, Yiu-Kuen T. Lam, Lawrence Koupal
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Patent number: 5229278Abstract: Microorganisms which are capable of growing with 2,5-dimethylpyrazine as the sole carbon, nitrogen and energy source. These microorganisms hydroxylate heterocycles of general formula: ##STR1## to heterocycles of general formula: ##STR2## The latter compounds are accumulated in the growth medium.Type: GrantFiled: September 24, 1991Date of Patent: July 20, 1993Assignee: Lonza Ltd.Inventors: Andreas Kiener, Yvonne V. Gameren, Michael Bokel
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Patent number: 5217884Abstract: A process using microorganisms which contain genes, which form an active xylene monooxygenase, which form no effective, chromosomally or plasmid-coded alcohol hydrogenase, and which are, thus, capable of hydroxylating methyl groups on aromatic 5- or 6-atom heterocycles to the corresponding hydroxymethyl derivatives, for the production of hydroxymethylated 5- or 6-atom heterocycles.Type: GrantFiled: September 23, 1991Date of Patent: June 8, 1993Assignee: Lonza Ltd.Inventors: Thomas Zimmermann, Andreas Kiener, Shigeaki Harayama
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Patent number: 5217885Abstract: A novel antitumor substance BE-13793C or a pharmaceutically acceptable salt thereof, which is represented by the following formula: ##STR1## an anticancer agent comprising the novel substance or a pharmaceutically acceptable salt thereof, a method of producing the novel substance and a microorganism capable of producing the novel substance are disclosed.Type: GrantFiled: January 21, 1992Date of Patent: June 8, 1993Assignee: Banyu Pharmaceutical Co., Ltd.Inventors: Hiroyuki Suda, Katuhisa Kojiri, Akira Okura, Kenji Kawamura, Masanori Okanishi
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Patent number: 5215900Abstract: Fermentation of the microorganism Streptomyces sp. (MA6804), ATTC No. 55095, in the presence of the HIV reverse transcriptase inhibitor ##STR1## yields a 5-(1-hydroxy)ethyl analog which is useful in the prevention or treatment of infection by HIV and the treatment of AIDS.Type: GrantFiled: June 7, 1991Date of Patent: June 1, 1993Assignee: Merck & Co., Inc.Inventors: Shieh-Shung T. Chen, George Doss
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Patent number: 5213973Abstract: A microbiological process for oxidation of methyl groups in aromatic 5- or 6-ring heterocycles to the corresponding carboxylic acid. The reaction of the heterocycle takes place by microorganisms of the genus Pseudomonas, which utilize toluene, xylene or cymene. An inducer, the aromatic heterocycle, as the substrate, and a carbon source and energy source are fed in and, after the maximal product concentration is reached, the product is separated.Type: GrantFiled: July 3, 1991Date of Patent: May 25, 1993Assignee: Lonza Ltd.Inventor: Frans Hoeks
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Patent number: 5187094Abstract: A method for the preparation of optically active 3-hydroxypyrrolidine derivatives is disclosed, which comprises the steps of: hydrolyzing a mixture of (R)- and (S)-N-benzyl-3-acyloxypyrrolidine by the use of microorganisms or enzymes with stereospecific esterase activity; and separating optically active N-benzyl-3-hydroxypyrrolidine and optically active N-benzyl-3-acyloxypyrrolidine from the hydrolysate obtained in the foregoing step.Type: GrantFiled: September 24, 1991Date of Patent: February 16, 1993Assignee: Kanegafuchi Kagaku Kogyo Kabushiki KaishaInventors: Ikuo Sawa, Natsuki Mori, Shunichi Maemoto, Hidetoshi Kutsuki, Junzo Hasegawa
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Patent number: 5182206Abstract: A novel pyrimine-producing strain belonging to genus Pseudomonas exhibits the following bacteriological properties: denitrification reaction: negative; assimilation of carbon sources:D-arabinose: positiveL-lysine: negativeand a novel pyrimine-producing strain belonging to genus Pseudomonas exhibits the following bacteriological properties: denitrification reaction: negative, assimilation of carbon sources:D-arabinose: positiveL-lysine: positiveThese novel strains produce pyrimine in high yield and if the strains are cultured in a proper culture medium in the presence of an iron salt, a natural red dye, ferropyrimine, can be easily produced and directly be recovered from the culture medium.Type: GrantFiled: December 10, 1991Date of Patent: January 26, 1993Assignee: House Food Industrial Co., Ltd.Inventors: Masanori Yamamoto, Toshio Nakayama, Osamu Fujii, Rie Okabe
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Patent number: 5179009Abstract: The present invention is directed to a process for producing L-aspartyl-L-phenylalanine and/or L-aspartyl-L-phenylalanine diketopiperazine comprising contacting and reacting a culture, cell or a cell-treated product of a microorganism capable of hydrolyzing DKP into AP or capable of performing intramolecular condensation of AP into DKP with DKP and/or AP, in an aqueous medium.Type: GrantFiled: May 29, 1991Date of Patent: January 12, 1993Assignee: Ajinomoto Co., Inc.Inventors: Kenzo Yokozeki, Naoki Usui, Toshihide Yukawa, Yoshiteru Hirose, Koji Kubota
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Patent number: 5177006Abstract: A novel enzymatic resolution process for preparing resolved compounds of the formula ##STR1## with improved yields and high optical purity is disclosed. Compounds of formula I' are useful, for example, as intermediates for the preparation of physiologically active compounds, e.g. captopril, zofenopril and endopeptidase inhibitor.Type: GrantFiled: July 2, 1991Date of Patent: January 5, 1993Assignee: E. R. Squibb & Sons, Inc.Inventors: Jeffrey M. Howell, Ramesh N. Patel, Laszlo J. Szarka
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Patent number: 5166060Abstract: Pyridine-2,3-dicarboxylic acids are prepared by the action of 2,3-dihydroxybenzoate-3,4-dioxygenase on 2,3-dihydroxybenzoic acids in a liquid medium which lacks active decarboxylase and which has a pH from 4 to 9, an ionic strength below about 1 molar, and a low concentration of metal cations and complex anions. In close temporal proximity, the 2-hydroxy-3-carboxymuconic acid semialdehyde which forms is allowed to react with a source of ammonia or a primary amine, avoiding substantial decarboxylation.Type: GrantFiled: March 31, 1989Date of Patent: November 24, 1992Assignee: Celgene CorporationInventors: Randall A. Roehl, George W. Matcham, David I. Stirling
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Patent number: 5155028Abstract: In the enzymatic stereoselective ester cleavages of 2-arylpropionate, the reaction rate of the hydrolyzing enzymes can be drastically increased if the vinyl ester of the 2-arylpropionate is employed as the substrate.Type: GrantFiled: June 8, 1990Date of Patent: October 13, 1992Assignee: Hoechst AktiengesellschaftInventors: Gerd Fulling, Merten Schlingmann, Reinhold Keller
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Patent number: 5151351Abstract: A process for the production of 6-hydroxynicotinic acid from nicotinic acid by means of enzymatic hydroxylation in the presence of microorganisms of the genera Pseudomonas, Bacillus or Achromobacter. By maintaining a specific concentration range during the addition of nicotinic acid, the biomass formation can take place in the same process step as the product formation, without product losses occurring by further decomposition.Type: GrantFiled: May 24, 1991Date of Patent: September 29, 1992Assignee: Lonza Ltd.Inventors: Frans Hoeks, Daniel Venetz
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Patent number: 5135858Abstract: An improved biological conversion of a nitrile such as acrylonitrile or a cyanopyridine into the corresponding carboxylic acid such as acrylic acid or a nicotinic acid by the action upon the nitrile of a nitrilase enzyme, in which the improvement resides in the use as the source of the enzyme of a microorganism of Rhodococcus, such as Rh. rhodochrous J-1, FERM BP-1478, which is cultured in the presence of a lactam compound.Type: GrantFiled: February 22, 1991Date of Patent: August 4, 1992Assignees: Hideaki Yamada, Nitto Kagaku Kogyo Kabushiki KaishaInventors: Hideaki Yamada, Toru Nagasawa, Tetsuji Nakamura
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Patent number: 5126254Abstract: A method for producing streptovaricin by culturing a streptovaricin producing strain in the presence of a nonionic adsorbent. This procedure substantially increases the production efficiency of the streptovaricin. A second embodiment wherein the streptovaricin producing strain is produced in the presence of fumaric acid or its water soluble salts to provide improvements in production efficiency is also disclosed.Type: GrantFiled: October 23, 1990Date of Patent: June 30, 1992Assignees: Shin-Etsu Chemical Co., Ltd., The Institute of Physical Chemical ResearchInventors: Isao Endo, Shigehiro Nagura, Kaname Inoue, Jun Watanabe
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Patent number: 5122461Abstract: The invention relates to a novel method for preparing pyrocatecholic compounds from monophenolic compounds using catalysis by monophenol monooxygenase in an aqueous solution containing metal ions. The metal ions form complexes with the pyrocatecholic products. This method increases the yield of pyrocatecholic products. In particular, the method of the instant invention can be used to increase the yield of L-DOPA, a drug used in the treatment of Parkinson's disease.Type: GrantFiled: November 19, 1990Date of Patent: June 16, 1992Assignee: Industrial Technology Research InstituteInventors: Kuang-Pin Hsiung, Feng-Tsun Lee, Chung-Long Hsieh
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Patent number: 5112747Abstract: The invention relates to a process for the preparation of indole applicable in flavoring and perfume compositions wherein a micro-organism which does not or hardly metabolize indole is cultured aerobically or anaerobically in a culture medium containing as the substrate tryptophan of natural origin. The produced indole in the fermentation broth may be isolated therefrom with a food grade extraction agent.Type: GrantFiled: February 20, 1991Date of Patent: May 12, 1992Assignee: Unilever Patent Holdings BVInventors: Adrianus M. Van Grinsven, Alfons L. J. Peters, Robert Roos
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Patent number: 5109133Abstract: Novel compounds of the formula ##STR1## wherein R is hexahydrobenzoyl, isovaleryl or 2-methylbutyryl, and pharmaceutically acceptable salts thereof. The novel compounds designated as Trienomycins A, B and C are produced by culturing a microorganism belonging to genus Streptomyces, specifically Streptomyces sp. 82-16 FERM BP-939 in a medium, accumulating the new compounds in the medium and isolating the new compounds therefrom, and if required converted to a salt thereof. These compounds have antitumor activity and cytocidal activity.Type: GrantFiled: January 27, 1986Date of Patent: April 28, 1992Assignee: The Kitasato InstituteInventor: Shinji Funayama
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Patent number: 5108924Abstract: A novel pyrimine-producing strain belonging to genus Pseudomonas exhibits the following bacteriological properties: denitrification reaction: negative; assimilation of carbon sources:D-arabinose: positiveL-lysine: negativeand a novel pyrimine-producing strain belonging to genus Pseudomonas exhibits the following bacteriological properties: dentrification reaction: negative; assimilation of carbon sources:D-arabinose: positiveL-lysine: positiveThese novel strains produce pyrimine in high yield and if the strains are cultured in a proper culture medium in the presence of an iron salt, a natural red dye, ferropyrimine, can be easily produced and directly be recovered from the culture medium.Type: GrantFiled: June 26, 1990Date of Patent: April 28, 1992Assignee: House Food Industrial Co., Ltd.Inventors: Masanori Yamamoto, Toshio Nakayama, Osamu Fujii, Rie Okabe
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Patent number: 5106736Abstract: An enzymatic process for the enantiomer-specific preparation of mercapto alkanoic acids by stereoselective hydrolysis of mercapto or thioester alkanoic acid esters.Type: GrantFiled: April 23, 1991Date of Patent: April 21, 1992Assignee: E.R. Squibb & Sons, Inc.Inventors: Ramesh N. Patel, Laszlo J. Szarka
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Patent number: 5104798Abstract: A microbiological process for the oxidation of methyl groups in aromatic 5- or 6-member ring heterocycles to the corresponding carboxylic acid. The heterocycle serves as the substrate and exhibits no substituent on the carbon atoms adjacent to the methyl groups to be oxidized. The reaction of the heterocycle takes place by means of microorganisms of the genus Pseudomonas utilizing toluene, xylene, or cymene. The enzymes of the microorganisms have been previously induced.Type: GrantFiled: February 5, 1991Date of Patent: April 14, 1992Assignee: Lonza Ltd.Inventor: Andreas Kiener
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Patent number: 5098836Abstract: In a process for polymerizing monomers with free-radical initiators in aqueous solution to produce polymers useful in oil field applications, wherein said polymerization process employs an oxygen-scavenging agent, the improvement which comprises employing said oxygen scavenging agent and oxidase enzyme with a substrate therefor, optionally with a catalase, such as alcohol oxidase and methanol, to consume dissolved oxygen in a polymerization admixture. Oxygen-scavenging under field conditions with the oxidase/substrate system permits consistent production conveniently of water based polymer solutions of good viscosity for oil field applications.Type: GrantFiled: August 14, 1989Date of Patent: March 24, 1992Assignee: Phillips Petroleum CompanyInventors: G. Allan Stahl, Thomas R. Hopkins
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Patent number: 5085992Abstract: Fermentation of the microorganism Streptomyces sp. MA6751 (ATCC No. 55043) in the presence of the Angiotensin II (A II) receptor antagonist ##STR1## yields an N2-tetrazole .beta.-glucuronide analog which is also an A II antagonist useful in the treatment of hypertension and congestive heart failure and other indications known to respond to A II antagonists.Type: GrantFiled: February 25, 1991Date of Patent: February 4, 1992Assignee: Merck & Co., Inc.Inventors: Shieh-Shung T. Chen, George Doss
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Patent number: 5079148Abstract: Novel peptides of the formula ##STR1## wherein R is CH.sub.3 --(CH.sub.2).sub.6, CH.sub.3 --(CH.sub.2).sub.4 --CH.dbd.CH--(CH.sub.2).sub.2 and CH.sub.3 (CH.sub.2).sub.8 having antibiotic and antitumor activity are prepared by cultivation of the novel microorganism Polyangium brachysporum. Enzymatic hydrolysis of those peptides gives other peptides useful as intermediates in the preparation of peptides having activity as antibiotics and/or antitumor agents.Type: GrantFiled: September 7, 1990Date of Patent: January 7, 1992Assignee: Bristol-Myers Squibb CompanyInventors: Masataka Konishi, Koji Tomita, Masahisa Oka, Ken-ichi Numata
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Patent number: 5073549Abstract: Disclosed herein are antibiotic BU-4164E A and B isolated from Streptomyces sp. These compounds are prolyl endopeptidase inhibitors.Type: GrantFiled: October 4, 1990Date of Patent: December 17, 1991Assignee: Bristol-Myers Squibb CompanyInventors: Soichiro Toda, Yasutaro Hamagishi, Toshikazu Oki, Koji Tomita
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Patent number: 5063154Abstract: A yeast promoter inducible by the appropriate pheromone and a method of expressing a gene of interest in substantial quantities by placing it under the control of the inducible promoter. DNA encoding a protein of interest is fused or linked to a pheromone - inducible yeast promoter, such as the FUSI or the FUS2 promoter, and the fusion is inserted onto a high copy vector; the resulting product is introduced into wild type yeast cells. Stimulation of these yeast cells by the appropriate pheromone results in induction of transcription of the yeast promoter and expression of the protein of interest in substantial quantities.Type: GrantFiled: June 24, 1988Date of Patent: November 5, 1991Assignee: Whitehead Institute for Biomedical ResearchInventors: Gerald R. Fink, Joshua Trueheart, Elaine A. Elion
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Patent number: 5026642Abstract: In a process for the reduction of ketones, the keto compounds are enantioselectively reduced to give secondary alcohols by means of microorganisms. Keto compounds of the formulaR.sup.3 NH--CHR.sup.1 --CO--CH.sub.2 --COOR.sup.2in which R.sup.1, R.sup.2 and R.sup.3 have the meanings defined herein are used as starting compounds.Type: GrantFiled: July 22, 1988Date of Patent: June 25, 1991Assignee: Merck Patent Gesellschaft mit beschrankter HaftungInventors: Hans-Eckart Radunz, Harry Schwartz, Martin Heinrich
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Patent number: 4999349Abstract: Disclosed herein are antibiotic BU-4164E A and B isolated from Streptomyces sp. These compounds are prolyl endopeptidase inhibitors.Type: GrantFiled: March 22, 1990Date of Patent: March 12, 1991Assignee: Bristol-Myers Squibb Co.Inventors: Soichiro Toda, Yasutaro Hamagishi, Toshikazu Oki, Koji Tomita
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Patent number: 4916063Abstract: Novel peptide antibiotics designated herein as BU-2867T F and G are produced by fermentation of Polyangium brachysporum strain K481-B101 (ATCCC 53080). The new antibiotics are found to have antifungal activity and to inhibit P388 lymphatic leukemia in rodents.Type: GrantFiled: September 14, 1987Date of Patent: April 10, 1990Assignee: Bristol-Myers CompanyInventors: Masahisa Oka, Masataka Konishi
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Patent number: 4912215Abstract: A Q-1047 substance of the general formula (I) and methods of producing the same, which has antioxidant activity and are useful as prophylactic and therapeutic agents for nephritis.Type: GrantFiled: April 12, 1989Date of Patent: March 27, 1990Assignee: Yamanouchi Pharmaceutical Co., Ltd.Inventors: Hidenori Yazawa, Harumitsu Imai, Kenichi Suzuki, Shigenobu Kadota, Takeshi Saito
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Patent number: 4898822Abstract: A process for preparing optically active indoline-2-carboxylic acid by an optical resolution, which comprises subjecting a racemic ester of (R,S)-indoline-2-carboxylic acid having the general formula [(R,S)-I] to the action of an enzyme or a microorganism having a stereo-selective esterase activity, which is capable of asymmetrically hydrolyzing the racemic ester [(R,S)-I] to give optically active indoline-2-carboxylic acid having the formula [II*] so as to produce the hydrolysis product, i.e. optically active indoline-2-carboxylic acid [II*] and an unreacted optically active ester of indoline-2-carboxylic acid having the general formula [I*], isolating each optically active form, and further, if necessary, hydrolyzing the obtained optically active ester [I*] to give an optical antipode of the acid [II*].According to the process of the present invention, optically active indoline-2-carboxylic acid with a high optical purity can be prepared in a simple process with a good yield.Type: GrantFiled: March 31, 1986Date of Patent: February 6, 1990Assignee: Kanegafuchi Kagaku Kogyo Kabushiki KaishaInventors: Masanori Asada, Shigeki Hamaguchi, Hidetoshi Katsuki, Yoshio Nakamura, Hideyuki Takahashi, Kenji Takahara, Yoshio Shimada, Takehisa Ohashi, Kiyoshi Watanabe
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Patent number: 4870172Abstract: A novel compound having anti-tumor activity is disclosed which is 1,12-dihydroxy-1,6,12,17-tetraazacyclodocosan-2,5,13,16-tetrone produced microbiologically by culturing a microorganism belonging to the genus of Alteromonas or, in particular, Alteromonas haloplanktis SB-1123. The characterization data of the compound and the microbiological properties of the microorganism are described. The results of in vitro assays are given for the anti-tumor activity of the compound against L-1210 and IMC carcinoma cells.Type: GrantFiled: July 16, 1987Date of Patent: September 26, 1989Assignee: Microbial Chemistry Research FoundationInventors: Yoshiro Okami, Shogo Kurasawa, Toshiyuki Kamayama, Atsushi Takahashi, Masaaki Ishizuka, Hamao Umezawa, deceased
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Patent number: 4847284Abstract: A novel pyrrolidinol isolated from the fermentation of Aspergillus ochraceus and certain derivatives thereof are described. The compounds are broad spectrum antifungal agents.Type: GrantFiled: March 23, 1988Date of Patent: July 11, 1989Assignee: Merck & Co., Inc.Inventors: Robert E. Schwartz, Janet C. Onishi, Richard L. Monaghan, Jerrold M. Liesch, Otto D. Hensens
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Patent number: 4812406Abstract: A process for preparing optically active hydantoins having the general formula (II): ##STR1## wherein R.sup.1 and R.sup.2, which are different from each other, are independently alkyl group, aralkyl group, aryl group, substituted alkyl group, substituted aralkyl group, or substituted aryl group, or R.sup.1 and R.sup.2 form an asymmetric cyclic compound, characterized in that one configuration of racemic N-carbamoyl-.alpha.-amino acid having the general formula (I): ##STR2## wherein R.sup.1 and R.sup.2 are as above, is enzymatically converted into the corresponding hydantoins.The present invention provides a process for an optical resolution with a high efficiency which can be used for the synthesis of (S)-6-fluoro-spiro-[chroman-4,4'-imidazolidine]-2',5'-dione (USAN; Sorbinil), which is an optically active hydrantoins attracting public attention as a preventive or a remedy for the particular chronic symptoms of diabetes such as cataract and neuropathy, and (S)-.alpha.Type: GrantFiled: September 12, 1985Date of Patent: March 14, 1989Assignee: Kanegafuchi Kagaku Kogyo Kabushiki KaishaInventors: Satomi Takahashi, Yukio Yamada, Yasuyoshi Ueda, Yasuhiro Katayama, Yoshio Shimada, Kiyoshi Watanabe
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Patent number: 4804676Abstract: Propioxatins A and B, which have the formula: ##STR1## wherein R represents a hydrogen atom or a methyl group, can be prepared by cultivating a suitable strain of Kitasatosporia, e.g. Kitasatosporia sp. SANK 60684 (FERM-P 7581). They can be salified to give pharmaceutically acceptable salts. The compounds are active as enkephalinase B inhibitors and are thus capable of enhancing enkephalin activity in vivo.Type: GrantFiled: October 1, 1985Date of Patent: February 14, 1989Assignee: Sankyo Company LimitedInventors: Yoshinori Inaoka, Shuji Takahashi, Hidetsune Tamaoki, Ryuzo Enokita
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Patent number: 4803217Abstract: Hapalindolinone compounds are produced by the controlled aerobic fermentation of a cyanobacterium of the genus Fischerella, ATCC No. 53558.Type: GrantFiled: December 24, 1986Date of Patent: February 7, 1989Assignee: Merck & Co., Inc.Inventors: Robert E. Schwartz, Charles F. Hirsch, Janet M. Sigmund, Douglas J. Pettibone
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Patent number: 4783402Abstract: A process for producing a primary or secondary alcohol derivative of a phosopholipid which comprises reacting the phospholipid with a primary or secondary alcohol in the presence of phospholipase DM.Type: GrantFiled: April 10, 1984Date of Patent: November 8, 1988Assignee: Meito Sangyo Kabushiki KaishaInventors: Yoshitaka Kokusho, Shigeaki Kato, Haruo Machida
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Patent number: 4782019Abstract: A process for producing a sphingophospholipid derivative comprising reacting a sphingophospholipid with a specified compound having an alcoholic hydroxyl group selected from the group consisting of specified primary alcohol compounds, specified secondary alcohol compounds and specified saccharides or their phenol glycosides in the presence of phospholipase DM.Type: GrantFiled: April 10, 1984Date of Patent: November 1, 1988Assignee: Meito Sangyo Kabushiki KaishaInventors: Yoshitaka Kokusho, Shigeaki Kato, Haruo Machida
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Patent number: 4755610Abstract: New alkaloids called hapalindoles, which are antibacterial and antifungal agents, and methods of preparing these alkaloids by culturing the blue-green alga Hapalosiphon fontinalis ATCC 39694, are provided.Type: GrantFiled: February 14, 1986Date of Patent: July 5, 1988Assignee: University of HawaiiInventors: Richard E. Moore, Gregory M. L. Patterson
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Patent number: 4752301Abstract: A biomass containing biologically produced indigo or a derivative thereof which is used for dyeing without isolating the indigo dye from the rest of the solid biomass before dyeing. Cotton dyeings of clean pure shade can be obtained by a vat dyeing procedure which have the usual fastness properties associated with the active substance.Type: GrantFiled: June 23, 1987Date of Patent: June 21, 1988Assignee: Sandoz Ltd.Inventor: Werner Koch
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Patent number: 4738924Abstract: Process for the production of 6-hydroxynicotinic acid by biotechnological methods. Nicotinic acid is enzymatically hydroxylated in the presence of equivalent quantities of magnesium or barium ions with the help of nicotinic acid-hydroxylating microorganisms. The resultant magnesium or barium salt of the 6-hydroxynicotinic acid is separated from the reaction mixture. Then the 6-hydroxynicotinic acid is freed from the separated salts. Examples of the microorganism are the species Pseudomonas, Bacillus or Achromobacter, for example, Achromobacter xylosoxydans. Preferably the enzymatic hydroxylation is carried out at 20.degree. to 40.degree. C. and a pH of 5.5 to 9.0 under aerobic conditions.Type: GrantFiled: March 24, 1987Date of Patent: April 19, 1988Assignee: Lonza Ltd.Inventors: Hans Kulla, Pavel Lehky
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Patent number: 4738958Abstract: The present invention relates to the compound naphthomycin H of the formula I ##STR1## and to a microbiological process for its preparation. The compound is active against Gram-positive bacteria and fungi and can be used as an antibiotic.Type: GrantFiled: April 1, 1986Date of Patent: April 19, 1988Assignee: Hoechst AktiengesellschaftInventors: Christopher M. M. Franco, Goukanapalli C. S. Reddy, Triptikumar Mukhopadhyay, Bimal N. Ganguli, Hans-Wolfram Fehlhaber
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Patent number: 4717664Abstract: This invention provides for a method for producing secondary metabolites of higher plants, such as Lithospermum erythrorhizon, Coptis japonica, and Nicotinia tabacum, by suspension cultures of a mass of undifferentiated cells (callus). The cultures are carried out in at least two stages in liquid media. The first stage culturing is carried out in a liquid medium conventionally used for the tissue culture of plants, which contains indispensably inorganic substances and carbon sources and, additionally, phytohormones, vitamins and/or amino acids. While, the second stage culturing is carried out in another liquid medium, of which the concentration of at least one of the constituents is substantially varied, namely decreased or increased, from the first stage liquid medium. According to such two stage liquid medium cultures, the productivity of the second metabolites, such as shikonin, berberine and nicotine, are significantly increased, even though the rate of cell growth in the second stage is relatively low.Type: GrantFiled: August 16, 1985Date of Patent: January 5, 1988Assignee: Mitsui Petrochemical Industries, Ltd.Inventors: Yasuhiro Hara, Chuzo Suga
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Patent number: 4657861Abstract: The invention relates to the enzyme S-tetrahydroprotoberberineoxidase, which oxidizes selectively S-tetrahydroprotoberberines and S-1-benzyl-1,2,3,4-tetrahydroisoquinoline in the presence of oxygen, resulting in the corresponding protoberberines and 1-benzyl-3,4-dihydroisoquinolines. The invention also relates to a process of preparing S-tetrahydroprotoberberineoxidase by extraction from certain plant materials. The oxidation products of several compounds find use in the pharmaceutical industries.Type: GrantFiled: February 12, 1985Date of Patent: April 14, 1987Assignee: Consortium fur elektrochemische Industrie GmbHInventors: Manfred Amann, Naotaka Nagakura, Meinhart H. Zenk
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Patent number: 4649135Abstract: Mycotrienin-related compounds called the T-23-X, -XI, -XII, -XIII and -XIV. These compounds are isolated and characterized by structural formula and other identifying data. They are useful in the treatment of tumors.Type: GrantFiled: April 15, 1986Date of Patent: March 10, 1987Assignee: Nisshin Flour Milling Co., Ltd.Inventors: Noboru Otake, Haruo Seto, Tetsuo Sasaki, Masanori Sugita, Shigeru Hiramoto
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Patent number: 4600691Abstract: R-(Z)-4-amino-3-chloro-2-pentenedioic acid is a novel antibacterial and isolated from Streptomyces viridogenes MA5450, ATCC 39387.Type: GrantFiled: April 3, 1985Date of Patent: July 15, 1986Assignee: Merck & Co., Inc.Inventors: Louis Chaiet, Richard L. Monaghan, Sheldon B. Zimmerman, Maria I. M. Fernandez
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Patent number: 4588694Abstract: A process for preparing the optically active oxazolidinone derivative [(S)-I] by utilizing microorganisms or enzymes having a stereoselective esterase activity capable of asymmetrically hydrolyzing the racemates of the acyloxyoxazolidinone derivative [(R,S)-II], by separating the unreacted compound [(S)-II] from the hydrolyzed compound [(R)-I] and by hydrolyzing the compound [(S)-II]. The compounds are useful as intermediates for preparing optically active .beta.-adrenergic blocking agents.Type: GrantFiled: August 12, 1983Date of Patent: May 13, 1986Assignee: Kanegafuchi Kagaku Kogyo Kabushiki KaishaInventors: Shigeki Hamaguchi, Hiroshi Yamamura, Junzo Hasegawa, Hajime Kawaharada, Kiyoshi Watanabe
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Patent number: 4575489Abstract: Novel peptide B-52653 having the formula: ##STR1## which is produced by cultivating a microorganism belonging to the genus Streptomyces and being capable of producing B-52653 in a culture medium, whereby B-52653 is elaborated and accumulated in the cultured broth and is recovered.B-52653 is useful as a germicide or disinfectant, and a possibility of the present substance as an antifibrotic agent is suggested.Type: GrantFiled: September 8, 1981Date of Patent: March 11, 1986Assignee: Takeda Chemical Industries, Ltd.Inventors: Eiji Higashide, Satoshi Horii