Containing Six-membered Hetero Ring Patents (Class 435/122)
  • Patent number: 10703720
    Abstract: A method for preparing chromium(III) pyridine-2-carboxylate using 2-OP rectification residues, including: subjecting the 2-OP rectification residues to in-situ catalytic pyrolysis and vacuum distillation, room-temperature crystallization and separation, recrystallization and dehydration, alkali-catalyzed hydrolysis, acidification and complexation, and separation, washing and drying to prepare a chromium(III) pyridine-2-carboxylate product, of which a yield is greater than 50% of the weight of the 2-OP rectification residues. The acidification and complexation includes: cooling the aqueous solution containing sodium pyridine-2-carboxylate obtained from the alkali-catalyzed hydrolysis to a temperature below 50° C.; adjusting the aqueous solution to pH 3.0-8.5 with 5-50 wt % dilute H2SO4 or 3-30 wt % dilute HCl followed by batchwise addition of CrCl3, Cr(NO3)3 or Cr2(SO4)3 for complexation at 20-70° C.
    Type: Grant
    Filed: June 25, 2019
    Date of Patent: July 7, 2020
    Assignee: YANCHENG INSTITUTE OF TECHNOLOGY
    Inventors: Zhaosheng Cai, Jinghua Yu, Pan Xu, Jinyu Bian, Ge Ding, Lili Shen, Xujuan Huang, Zhonglie Yang, Yun Chen, Peng Xu
  • Patent number: 10465172
    Abstract: The present invention relates to peroxygenase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: December 1, 2014
    Date of Patent: November 5, 2019
    Assignee: Novozymes A/S
    Inventors: Jesper Vind, Lars Henrik Oestergaard, Leonardo DeMaria, Lisbeth Kalum, Eleni Amourgi
  • Patent number: 10407702
    Abstract: A novel process is provided for the efficient preparation of an asymmetric compound of structural formula I: employing dynamic kinetic resolution (DKR). The DKR process involves an enzymatic enantioselective amination reaction catalyzed by transaminases. The process can be used to manufacture key intermediates in the preparation of poly (ADP-ribose) polymerase (PARP) inhibitors which may be useful for the treatment of cancer.
    Type: Grant
    Filed: August 1, 2017
    Date of Patent: September 10, 2019
    Assignee: Merck Sharp & Dohme Corp.
    Inventors: Paul Gerard Bulger, Birgit Kosjek, Nelo Rivera
  • Patent number: 10301654
    Abstract: Provided is a method of preparing cinnamaldehyde by using a recombinant microorganism.
    Type: Grant
    Filed: May 13, 2016
    Date of Patent: May 28, 2019
    Assignee: INTELLIGENT SYNTHETIC BIOLOGY CENTER
    Inventors: Ki Jun Jeong, Sun Chang Kim, Hyun Bae Bang, Yoon Hyeok Lee, Suk Chae Jung
  • Patent number: 9790528
    Abstract: The present invention provides a method of producing lysergic acid and other ergot alkaloids by genetic modification of a fungus. A strain of fungus comprising Aspergillus fumigatus (A. fumigatus) and expressing one or more genes of the ergot alkaloid biosynthesis pathway from one or more fungus selected from the group consisting of Epichloë festucae var. lolii×Epichloë typhina isolate Lp1 (E. sp. Lp1); Claviceps species; Claviceps africana (C. africana); Claviceps gigantea (C. gigantea); Epichloë coenophiala and Periglandula species, wherein gene easA or gene easM is inactivated in said A. fumigatus, is provided.
    Type: Grant
    Filed: June 15, 2015
    Date of Patent: October 17, 2017
    Assignee: West Virginia University
    Inventors: Daniel G. Panaccione, Sarah L. Robinson
  • Patent number: 9738915
    Abstract: A novel process is provided for the efficient preparation of an asymmetric compound of structural formula I: employing dynamic kinetic resolution (DKR). The DKR process involves an enzymatic enantioselective amination reaction catalyzed by transaminases. The process can be used to manufacture key intermediates in the preparation of poly (ADP-ribose) polymerase (PARP) inhibitors which may be useful for the treatment of cancer.
    Type: Grant
    Filed: December 3, 2013
    Date of Patent: August 22, 2017
    Assignee: Merck Sharp & Dohme Corp.
    Inventors: Paul Gerard Bulger, Birgit Kosjek, Nelo Rivera
  • Patent number: 9611490
    Abstract: A method for producing cis-5-hydroxy-L-pipecolic acid is described. A gene recombinant microorganism enabling direct production of cis-5-hydroxy-L-pipecolic acid can be used in the method. Also described is a gene recombinant microorganism. In particular, it is described that a gene recombinant microorganism having DNAs encoding proteins involved in the biosynthesis of L-pipecolic acid and a DNA encoding a protein having the L-pipecolic acid cis-5-hydroxylase activity is cultured in a medium, and cis-5-hydroxy-L-pipecolic acid is collected from the medium.
    Type: Grant
    Filed: June 12, 2013
    Date of Patent: April 4, 2017
    Assignee: MicroBiopharm Japan Co., Ltd.
    Inventors: Tadashi Fujii, Keisuke Tamura
  • Patent number: 9410169
    Abstract: The present invention relates to an enantioselective enzymatic process for the preparation of an optically active 3-hydroxypiperidinecarboxylic acid derivative.
    Type: Grant
    Filed: June 18, 2012
    Date of Patent: August 9, 2016
    Assignee: LABORATORIO CHIMICO INTERNAZIONALE S.P.A.
    Inventors: Giorgio Bertolini, Paolo Magri′
  • Patent number: 9382559
    Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: August 10, 2012
    Date of Patent: July 5, 2016
    Assignee: Novozymes A/S
    Inventors: Sara Landvik, Lars H. Oestergaard, Lisbeth Kalum
  • Publication number: 20150148545
    Abstract: Disclosed in the present invention is a protein tyrosine phosphatase inhibitor. The preparation method therefor is: extracting the crude product from the Isaria Fumosorosea Wize solid or liquid fermentation broth using ethyl acetate, ethanol, methanol, or a mixed solvent of chloroform and methanol; separating the obtained extract using column chromatography on silica gel; and obtaining the target product. The inhibitor can be used to prepare pharmaceutical compositions for treating and preventing diabetes, obesity and cancers.
    Type: Application
    Filed: May 7, 2013
    Publication date: May 28, 2015
    Inventors: Duqiang Luo, Jun Zhang, Zhiqin Liu
  • Patent number: 9029113
    Abstract: Provided is a method for efficiently producing from a ketone compound an optically active amino compound useful as an intermediate of a drug, an agricultural chemical, or the like. Provided are: a polypeptide having aminotransferase activity that is increased in stereoselectivity, heat resistance, and resistance to amine compounds compared to the wild type enzyme by means of modifying an aminotransferase derived from Pseudomonas fluorescens; a gene encoding the polypeptide; and a transformant that expresses the gene at a high level.
    Type: Grant
    Filed: March 9, 2012
    Date of Patent: May 12, 2015
    Assignee: Kaneka Corporation
    Inventors: Noriyuki Ito, Shigeru Kawano, Yoshihiko Yasohara
  • Publication number: 20150118719
    Abstract: The present disclosure provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents. The present disclosure provides engineered proline hydroxylase biocatalysts, polynucleotides encoding the biocatalysts. methods of their preparation, and processes for preparing hydroxylated compounds using these engineered biocatalysts.
    Type: Application
    Filed: May 7, 2013
    Publication date: April 30, 2015
    Inventors: Haibin Chen, Yong Koy Bong, Fabien L. Cabirol, Anupam Gohel Prafulchandra, Tao Li, Jeffrey C. Moore, Martina Quintanar-Audelo, Yang Hong, Steven J. Collier, Derek Smith
  • Patent number: 8993280
    Abstract: The present invention provides a method for producing an alkaloid, for example, reticuline, comprising providing dopamine as a substrate for a series of actions of monoamine oxidase, norcoclaurine-6-O-methyltransferase, coclaurine-N-methyltransferase and 3?-hydroxy-N-methylcoclaurine-4?-O-methyltransferase.
    Type: Grant
    Filed: June 12, 2008
    Date of Patent: March 31, 2015
    Assignee: Kyoto University
    Inventors: Fumihiko Sato, Hiromichi Minami
  • Patent number: 8975063
    Abstract: The present invention relates to host cells that produce compounds that are characterized as benzylisoquinolines, as well as select precursors and intermediates thereof. The host cells comprise one, two or more heterologous coding sequences wherein each of the heterologous coding sequences encodes an enzyme involved in the metabolic pathway of a benzylisoquinoline, or its precursors or intermediates from a starting compound. The invention also relates to methods of producing the benzylisoquinoline, as well as select precursors and intermediates thereof by culturing the host cells under culture conditions that promote expression of the enzymes that produce the benzylisoquinoline or precursors or intermediates thereof.
    Type: Grant
    Filed: October 19, 2007
    Date of Patent: March 10, 2015
    Assignee: California Institute of Technology
    Inventors: D. Christina Smolke, Kristy Hawkins
  • Publication number: 20150037850
    Abstract: The present invention relates to a quinolinic acid-producing recombinant microorganism expressing a fusion protein of L-aspartate oxidase and quinolinate synthase linked via a linker, and a method for producing quinolinic acid using the same.
    Type: Application
    Filed: January 3, 2013
    Publication date: February 5, 2015
    Inventors: Yong Uk Shin, So Young Kim, In Kyung Heo, Ju Eun Kim, Sung Kwang Son, Jae Hee Lee, Ji Hyun Lee, Chang Gyeom Kim
  • Publication number: 20150031093
    Abstract: To produce quinolinate effectively, a L-aspartate oxidase variant that the feedback regulation by nicotinic acid or NAD is released, and a microorganism including the L-aspartate oxidase variant are provided. Quinolinate may be effectively produced by culturing of the microorganism including the L-aspartate oxidase variant.
    Type: Application
    Filed: July 25, 2014
    Publication date: January 29, 2015
    Inventors: So Young KIM, Yong Uk SHIN, In Kyung HEO, Ju Eun KIM, Kwang Ho NA, Chang Il SEO, Sung Kwang SON, Jae Hee LEE
  • Patent number: 8921079
    Abstract: The present disclosure relates to methods of using transaminase polypeptides in the synthesis of chiral amines from prochiral ketones.
    Type: Grant
    Filed: June 21, 2010
    Date of Patent: December 30, 2014
    Assignee: Codexis, Inc.
    Inventors: Gregory Hughes, Paul N. Devine, Fred J. Fleitz, Brendan T. Grau, John Limanto, Christopher Savile, Emily Mundorff
  • Publication number: 20140349350
    Abstract: The present invention relates to an improved process for the preparation of compound of Formula-II, which is an intermediate in the preparation of HMG-CoA reductase inhibitors. wherein X is hydrogen or hydroxy protecting group and R1 is carboxyl protecting group.
    Type: Application
    Filed: May 28, 2014
    Publication date: November 27, 2014
    Inventors: Michiel Christian Alexander Van Vliet, Willem Robert Klass Schoevaart, Madhuresh Kumar Sethi, Sanjay Mahajan, Bhairaiah Mara
  • Publication number: 20140342412
    Abstract: The present disclosure relates to transaminase polypeptides capable of aminating a dicarbonyl substrate, and polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides.
    Type: Application
    Filed: September 7, 2012
    Publication date: November 20, 2014
    Applicant: CODEXIS, INC.
    Inventors: Fabien Cabirol, Haibin Chen, Anupam Gohel, Paulina Salim, Derek Smith, Jacob Janey, Birgit Kosjek, Weng Lin Tang, Helen Hsieh, Son Pham
  • Patent number: 8877475
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.
    Type: Grant
    Filed: September 11, 2012
    Date of Patent: November 4, 2014
    Assignee: Codexis, Inc.
    Inventors: Christopher Savile, John M. Gruber, Emily Mundorff, Gjalt Huisman, Steven James Collier
  • Publication number: 20140322767
    Abstract: The present invention relates to a droplet microreactor, i.e. a microreactor consisting of a droplet of a specific liquid, the microreactor being wall-less, wherein the interface of the specific liquid with the ambient environment and with the support on which the droplet is deposited defines the limits of the microreactor. The microreactor is characterized in that it consists of a droplet comprising at least one ionic liquid. The present invention also relates to methods for carrying out chemical or biochemical reactions and/or mixes using said droplet microreactor, and also to a lab-on-chip comprising a microreactor according to the invention.
    Type: Application
    Filed: May 6, 2014
    Publication date: October 30, 2014
    Inventors: Gilles MARCHAND, Françoise VINET, Guillaume DE LAPIERRE, Fatima HASSINE, Saïd GMOUH, Michel VAULTIER
  • Publication number: 20140315263
    Abstract: A method for the preparation of nicotinic acid, which includes the step of obtaining a culture solution containing quinolinic acid by incubating a microorganism having an ability to produce quinolinic acid, and the step of adding an acid to the culture solution and conducting a decarboxylation reaction.
    Type: Application
    Filed: January 16, 2012
    Publication date: October 23, 2014
    Applicant: CJ CHEILJEDANG CORPORATION
    Inventors: So Young Kim, Yong Uk Shin, In Kyung Heo, Ju Eun Kim, Sung Kwang Son, Chang II Seo, Hyun Ah Kim, Han Jin Lee, Kwang Ho Na, Jee Yeon Bae
  • Publication number: 20140273109
    Abstract: Aspects of the invention include host cells that are engineered to produce benzylisoquinoline alkaloids (BIAs). The host cells include heterologous coding sequences for a variety of enzymes involved in synthetic pathways from starting compounds to BIAs of the host cell. Also provided are methods of producing the BIAs of interest by culturing the host cells under culture conditions that promote expression of enzymes encoded by the heterologous coding sequences of the host cells. Aspects of the invention further include compositions, e.g., host cells, starting compounds and kits, etc., that find use in methods of the invention.
    Type: Application
    Filed: March 14, 2014
    Publication date: September 18, 2014
    Inventors: Christina D. Smolke, Catherine Thodey, Isis Trenchard, Stephanie Galanie
  • Publication number: 20140256004
    Abstract: The present invention relates to a novel process for the preparation of a compound of formula (I) wherein R is a methyl or methoxy group; to certain novel intermediates prepared in such a process and their use.
    Type: Application
    Filed: October 19, 2012
    Publication date: September 11, 2014
    Applicant: CIPLA LIMITED
    Inventors: Dharmaraj Ramachandra Rao, Rajendra Narayanrao Kankan, Sanjay Naik, Maruti Ghagare, Sandip Vasant Chikhalikar
  • Publication number: 20140256003
    Abstract: Provided are R-praziquantel preparation methods, which utilize the characteristics of biological enzyme of strong stereoselectivity, site-selectivity, and regioselectivity, of high resolution efficiency, of mild reaction conditions, and of simple operations to catalyze the hydrolysis of a certain enantiomer in a chemically synthesized racemate or a derivative, thus acquiring a mixture of reacted and unreacted optical isomers. R-praziquantel prepared by the method can have a purity of 98% or more.
    Type: Application
    Filed: October 26, 2012
    Publication date: September 11, 2014
    Applicant: Suzhou Tongli Biomedical Co., Ltd.
    Inventor: Mingxin Qian
  • Publication number: 20140120587
    Abstract: A method comprising the steps (a) contacting a hydrocarbon comprising a hydroxyl group with a biological agent having oxygen-dependent and cofactor-dependent carbohydrate oxidase activity in the presence of oxygen and carbohydrate oxidase cofactor, and (b) contacting the hydrocarbon produced in step a) with a biological agent having transaminase activity and a biological agent having cofactor-dependent amino acid dehydrogenase activity in the presence of amino acid dehydrogenase cofactor and the substrate amino acid of the amino acid dehydrogenase.
    Type: Application
    Filed: January 10, 2012
    Publication date: May 1, 2014
    Applicant: Evonik Degussa GmbH
    Inventors: Thomas Haas, Jan Christoph Pfeffer, Kurt Faber, Michael Fuchs
  • Publication number: 20140020694
    Abstract: A method of modifying the content of certain chemical compounds in tobacco materials is provided, the method including treatment of a tobacco plant or portion thereof with at least one enzyme. For example, the method may modify the content of tobacco smoke toxicant precursors in tobacco materials, which can result in a modification in toxicant production when the tobacco material is exposed to elevated temperatures. The type of tobacco plant or portion thereof treated according to the invention can be, for example, a tobacco seed, a tobacco seedling, an immature live plant, a mature live plant, a harvested plant, or a plant derivative. Smoking articles and other tobacco products including such enzyme-treated tobacco materials are also provided.
    Type: Application
    Filed: July 19, 2012
    Publication date: January 23, 2014
    Inventors: Serban C. Moldoveanu, Jerry Wayne Marshall, Marvin Glenn Riddick, Michael F. Davis
  • Publication number: 20140011244
    Abstract: A method for producing naturally derived beneficial compounds including dispersing a microbiological culture media including at least one live probiotic organism, and at least one nutraceutical and/or at least one nutritive agent in distilled water to form a broth, incubating the broth at a predetermined temperature for a select period of time to induce probiotic activity; halting the probiotic activity, and separating the desired compound from the broth.
    Type: Application
    Filed: September 9, 2013
    Publication date: January 9, 2014
    Applicant: Premier Research Labs, LP
    Inventor: Robert J. Marshall
  • Publication number: 20140013465
    Abstract: The present invention relates to genetically modified plants of the species Papaver bracteatum wherein the type or amount of one or more alkaloids produced by the plants has been modified. Specifically, the genetically modified plants have an increased expression of one or more of thebaine 6-O-demethylase, codeine O-demethylase and/or codeinone reductase relative to wild type P. bracteatum such that the genetically modified poppy plants produce an increased quantity of an alkaloid selected from codeine, oripavine and/or morphine relative to a wild type P. bracteatum. Also provided are progeny plants having the genetically modified poppy plants described above as a parent; mutant or derivative plants of the aforementioned plants; reproductive material derived from, straw produced from, straw concentrate produced from, latex derived from, or one or more isolated cells derived from, the aforementioned plants.
    Type: Application
    Filed: March 15, 2013
    Publication date: January 9, 2014
    Applicant: TPI ENTERPRISES LTD.
    Inventors: Justin Taylor Coombs, Jarrod David Ritchie, Mark Alfred Tester, Damien Lightfoot, Deepa Jha
  • Publication number: 20140004575
    Abstract: Provided is a method for efficiently producing from a ketone compound an optically active amino compound useful as an intermediate of a drug, an agricultural chemical, or the like. Provided are: a polypeptide having aminotransferase activity that is increased in stereoselectivity, heat resistance, and resistance to amine compounds compared to the wild type enzyme by means of modifying an aminotransferase derived from Pseudomonas fluorescens; a gene encoding the polypeptide; and a transformant that expresses the gene at a high level.
    Type: Application
    Filed: March 9, 2012
    Publication date: January 2, 2014
    Applicant: Kaneka Corporation
    Inventors: Noriyuki Ito, Shigeru Kawano, Yoshihiko Yasohara
  • Patent number: 8617853
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
    Type: Grant
    Filed: December 19, 2011
    Date of Patent: December 31, 2013
    Assignee: Codexis, Inc.
    Inventors: Jack Liang, Birthe Borup, Vesna Mitchell, Emily Mundorff, James Lalonde, Gjalt Huisman
  • Publication number: 20130337485
    Abstract: The present invention discloses a process for preparing an active pharmaceutical ingredient (API) or intermediates thereof, notably particular step in the synthesis of an intermediate useful for example in the preparation of statins, by using an enzyme capable of catalyzing oxidation or dehydrogenation. The invention further provides an expression system effectively translating said enzyme. In addition, the invention relates to a specific use of such enzyme for preparing API or intermediate thereof, and in particular for preparing statin or intermediate thereof.
    Type: Application
    Filed: December 20, 2011
    Publication date: December 19, 2013
    Applicant: LEK PHARMACEUTICALS D.D
    Inventors: Peter Mrak, Tadeja Zohar, Matej Oslaj, Gregor Kopitar
  • Publication number: 20130302859
    Abstract: The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
    Type: Application
    Filed: May 9, 2013
    Publication date: November 14, 2013
    Applicant: CODEXIS,INC.
    Inventors: CHEN Haibin, Steven J. Collier, Jovana NAZOR, Joly SUKUMARAN, Derek SMITH, Jeffrey C. MOORE, Gregory HUGHES, Jacob JANEY, Gjalt HUISMAN, Scott NOVICK, Nicholas AGARD, Oscar ALVIZO, Gregory COPE, Wan Lin YEO, Stephanie NG
  • Publication number: 20130303723
    Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.
    Type: Application
    Filed: December 28, 2012
    Publication date: November 14, 2013
    Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Priti Pharkya
  • Patent number: 8551732
    Abstract: The present invention relates to newly identified mRNA stabilizing elements useful for the production of a target fermentation product, such as e.g. vitamins or enzymes, in particular riboflavin (vitamin B2), biotin, pantothenic acid (vitamin B5), folic acid, thiamin, pyridoxine (vitamin B6), vitamin B12, xylanase, amylase, protease, glucanase, amylomaltase or maltogenic amylase.
    Type: Grant
    Filed: June 9, 2008
    Date of Patent: October 8, 2013
    Assignee: DSM IP Assets B.V.
    Inventors: Martin Lehmann, Zoltan Pragai, Michéle Schaber
  • Publication number: 20130260426
    Abstract: The present invention relates to non-naturally occurring polypeptides useful for preparing armodafinil, polynucleotides encoding the polypeptides, and methods of using the polypeptides. The non-naturally occurring polypeptides of the present invention are effective in carrying out biocatalytic conversion of the (i) 2-(benzhydrylsulfinyl)acetamide to (?)-2-[(R)-(diphenyl-methyl)sulfinyl]acetamide (armodafinil), or (ii) benzhydryl-thioacetic acid to (R)-2-(benzhydrylsulfinyl)acetic acid, which is a pivotal intermediate in the synthesis of armodafinil, in enantiomeric excess.
    Type: Application
    Filed: December 7, 2011
    Publication date: October 3, 2013
    Inventors: Ee Lui Ang, Oscar Alvizo, Behnaz Behrouzian, Michael Clay, Steven Collier, Ellen Eberhard, Fan Jaslyn Fu, Shiwei Song, Derek Smith, Magnus Widegren, Robert Wilson, Junye Xu, Jun Zhu
  • Publication number: 20130196013
    Abstract: Compositions and methods for controlling molluscs, members of the Gastropoda and Bivalvia classes which includes but is not limited to lactones, lactams, carbamates, amides, and/or carboxylic acid containing compounds as active ingredients and/or compounds derived from Pseudomonas and/or Erwinia. Also provided are methods and compositions for increasing the efficacy of chemical and biological control for invasive molluscs in open waters, power plants, and drinking water treatment facilities under coldwater conditions.
    Type: Application
    Filed: March 15, 2013
    Publication date: August 1, 2013
    Applicant: MARRONE BIO INNOVATIONS, INC.
    Inventor: MARRONE BIO INNOVATIONS, INC.
  • Publication number: 20130189731
    Abstract: The present invention relates to a improved process for the biotechnological production of compounds for which ribose-5-phosphate, ribulose-5-phosphate or xylulose-5-phosphate is biosynthetic precursor like riboflavin (vitamin B2), FAD, FMN, pyridoxal phosphate (vitamin B6), guanosine, GMP, adenosine, AMP. The invention further pertains to the generation of the organism producing those compounds. It furthermore relates to the generation of mutated transketolases that allow normal growth on glucose but reduced growth on gluconate when introduced into the production strains and to polynucleotides encoding them.
    Type: Application
    Filed: July 26, 2012
    Publication date: July 25, 2013
    Applicant: DSM IP Assets B.V.
    Inventors: Michael Hans, Dietmar Laudert, Hans-Peter Hohmann, Martin Lehmann
  • Publication number: 20130121978
    Abstract: Compositions and methods for controlling molluscs, members of the Gastropoda and Bivalvia classes which includes but is not limited to lactones, lactams, carbamates, amides, and/or carboxylic acid containing compounds as active ingredients and/or compounds derived from Pseudomonas and/or Erwinia. Also provided are methods and compositions for increasing the efficacy of chemical and biological control for invasive molluscs in open waters, power plants, and drinking water treatment facilities under coldwater conditions.
    Type: Application
    Filed: December 17, 2012
    Publication date: May 16, 2013
    Applicant: MARRONE BIO INNOVATIONS, INC.
    Inventor: Marrone Bio Innovations, Inc.
  • Publication number: 20130123563
    Abstract: A self-decontaminating metal organic framework including an acid linked to a metal producing a metal organic framework configured for the sorption of chemical warfare agents and/or toxic industrial chemicals, the metal organic framework including reactive sites for the degradation of the agents and chemicals.
    Type: Application
    Filed: January 2, 2013
    Publication date: May 16, 2013
    Inventors: Yongwoo Lee, Thomasz Modzelewski, John P. Puglia, Steven E. Weiss, Banglin Chen
  • Publication number: 20130115662
    Abstract: The invention relates to polypeptides having peroxygenase activity and compositions comprising such polypeptides, their encoding polynucleotides, expression vectors and recombinant host cells comprising such polynucleotides or vectors, methods of producing the polypeptides, as well as methods of application and uses thereof, including a process for enzymatic, regioselective oxygenation of N-heterocycles of the general formula (I) to the corresponding N-oxides of the formula (II), by converting N-heterocycles of the formula (I) with a peroxidase polypeptide in the presence of at least one oxidizing agent in a one-stage reaction process.
    Type: Application
    Filed: December 20, 2012
    Publication date: May 9, 2013
    Applicant: NOVOZYMES A/S
    Inventor: Novozymes A/S
  • Patent number: 8367387
    Abstract: The invention relates to polypeptides having peroxygenase activity and compositions comprising such polypeptides, their encoding polynucleotides, expression vectors and recombinant host cells comprising such polynucleotides or vectors, methods of producing the polypeptides, as well as methods of application and uses thereof, including a process for enzymatic, regioselective oxygenation of N-heterocycles of the general formula (I) to the corresponding N-oxides of the formula (II), by converting N-heterocycles of the formula (I) with a peroxidase polypeptide in the presence of at least one oxidizing agent in a one-stage reaction process.
    Type: Grant
    Filed: March 31, 2008
    Date of Patent: February 5, 2013
    Assignee: Novozymes A/S
    Inventors: Marek Jan Pecyna, Kirk Matthew Schnorr, René Ullrich, Katrin Scheibner, Martin Gunter Kluge, Martin Hofrichter
  • Patent number: 8367361
    Abstract: A method of using O6-alkylguanine-DNA alkyltransferase (AGT) is disclosed for transferring a label from a substrate to a fusion protein comprising the AGT. This allows the detection and/or manipulating of the fusion protein, both in vitro and in vivo, by attaching molecules to the fusion proteins that introduce a new physical or chemical property to the fusion protein. Examples of such molecules are, among others, spectroscopic probes or reporter molecules, affinity tags, molecules generating reactive radicals, cross-linkers, ligands mediating protein-protein interactions or molecules suitable for the immobilisation of the fusion protein.
    Type: Grant
    Filed: January 24, 2011
    Date of Patent: February 5, 2013
    Assignee: Ecole Polytechnique Federale de Lausanne
    Inventors: Kai Johnsson, Susanne Kindermann (née Gendreizig), Antje Keppler
  • Patent number: 8349593
    Abstract: Disclosed is a novel process for producing a compound represented by the general formula (1) by using a microorganism, wherein the compound is useful as a plant growth stimulant. Specifically disclosed is a process for producing a succinic acid amide compound represented by the general formula (1) [wherein Ar represents a phenyl, naphthyl or indolyl group which may have a substituent; and n represents a numeral value ranging from 1 to 4] or a salt thereof, which is characterized by culturing a microorganism of the genus Pseudomonas that is capable of synthesizing a succinic acid amide in a culture medium containing an arylalkylamine represented by the general formula (2).
    Type: Grant
    Filed: October 24, 2008
    Date of Patent: January 8, 2013
    Assignee: Sumitomo Chemical Company, Limited
    Inventors: Toru Kitamura, Hiroshi Soejima, Tamizi Sugiyama
  • Patent number: 8338142
    Abstract: The present invention relates to a method for producing an optically active 3-aminopiperidine or salt thereof. In the method, a racemic nipecotamide is stereoselectively hydrolyzed to obtain an optically active nipecotamide and an optically active nipecotic acid in the presence of an enzyme source derived from an organism, and then the optically active nipecotamide is derived into an optically active aminopiperidine or salt thereof by aroylation, Hofmann rearrangement, deprotection of the amino group and further deprotection; or the optically active nipecotamide is derived into an optically active aminopiperidine or salt thereof by selective protection with BOC, Hofmann rearrangement and further deprotection. It is possible by the present invention to produce an optically active 3-aminopiperidine or salt thereof useful as a pharmaceutical intermediate from an inexpensive and easily available starting material by easy method applicable to industrial manufacturing.
    Type: Grant
    Filed: February 18, 2008
    Date of Patent: December 25, 2012
    Assignee: Kaneka Corporation
    Inventors: Kohei Mori, Masutoshi Nojiri, Akira Nishiyama, Naoaki Taoka
  • Patent number: 8288141
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.
    Type: Grant
    Filed: August 27, 2009
    Date of Patent: October 16, 2012
    Assignee: Codexis, Inc.
    Inventors: Christopher Savile, John M. Gruber, Emily Mundorff, Gjalt Huisman, Steven James Collier
  • Patent number: 8288132
    Abstract: The present invention discloses a chemoenzymatic process for the preparation of an iminocyclitol corresponding to formula (I), (II), (III) or (IV), wherein: R1 and R2 are the same or different, and independently selected from the group consisting of: H, OH, hydroxymethyl, methyl, ethyl, butyl, pentyl, hexyl, octyl, isopropyl, isobutyl, 2-methylbutyl, and benzyl; R3 is selected from the group consisting of: H, hydroxymethyl, hydroxyethyl, ethyl, butyl, pentyl, hexyl, octyl, dodecyl, isobutyl, isopropyl, isopentyl, 2-methylbutyl, benzyl, and phenylethyl; n: 0 or 1; the process comprising: (i) an aldol addition catalyzed by a D-fructose-6-phosphate aldolase enzyme (FSA) and an acceptor aminoaldehyde; and (ii) an intramolecular reductive amination of the addition adduct obtained in step (i) with H2, in the presence of a metallic catalyst, optionally being carried out said step (ii) in the presence of an aldehyde of formula R3—CHO, wherein R3 is as defined above.
    Type: Grant
    Filed: August 30, 2007
    Date of Patent: October 16, 2012
    Assignees: Consejo Superior de Investigaciones Cientificas, Bioglane, S.L.N.E.
    Inventors: Pere Clapés Saborit, Jesús Joglar Tamargo, José Antonio Castillo Expósito, Carles Lozano Pérez
  • Publication number: 20120196333
    Abstract: The process for the synthesis of statins featuring the use of an early intermediate (4R,6S)-6-(dialkoxymethyl)tetrahydro-2H-pyran-2,4-diol which already possesses the desired stereochemistry corresponding to the final statin.
    Type: Application
    Filed: March 12, 2012
    Publication date: August 2, 2012
    Applicant: LEK PHARMACEUTICALS D.D.
    Inventors: ZDENKO CASAR, TOMAZ MESAR, GREGOR KOPITAR, PETER MRAK, MATEJ OSLAJ
  • Publication number: 20120190085
    Abstract: The present disclosure relates to methods of using transaminase polypeptides in the synthesis of chiral amines from prochiral ketones.
    Type: Application
    Filed: June 21, 2010
    Publication date: July 26, 2012
    Applicant: CODEXIS, INC.
    Inventors: Gregory Hughes, Paul N. Devine, Fred J. Fleitz, Brendan T. Grau, John Limanto, Christopher Savile, Emily Mundorff
  • Patent number: 8227617
    Abstract: A process for preparing a 2-hydroxy-4-substituted pyridine compound using a microbiological method, a novel microorganism, and a novel compound are provided.
    Type: Grant
    Filed: August 11, 2010
    Date of Patent: July 24, 2012
    Assignee: Yuki Gosei Kogyo Co., Ltd.
    Inventors: Mie Sasaki, Seiichiro Matsumoto