Containing Six-membered Hetero Ring Patents (Class 435/122)
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Patent number: 11859225Abstract: A method of epimerizing an (S)-1-benzylisoquinoline alkaloid to an (R)-1-benzylisoquinoline alkaloid is provided. The method comprises contacting the (S)-1-benzylisoquinoline alkaloid with at least one enzyme. Contacting the (S)-1-benzylisoquinoline alkaloid with the at least one enzyme converts the (S)-1-benzylisoquinoline alkaloid to an (R)-1-benzylisoquinoline alkaloid.Type: GrantFiled: May 9, 2016Date of Patent: January 2, 2024Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Christina D. Smolke, Stephanie Galanie, Isis Trenchard, Catherine Thodey, Yanran Li
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Patent number: 10703720Abstract: A method for preparing chromium(III) pyridine-2-carboxylate using 2-OP rectification residues, including: subjecting the 2-OP rectification residues to in-situ catalytic pyrolysis and vacuum distillation, room-temperature crystallization and separation, recrystallization and dehydration, alkali-catalyzed hydrolysis, acidification and complexation, and separation, washing and drying to prepare a chromium(III) pyridine-2-carboxylate product, of which a yield is greater than 50% of the weight of the 2-OP rectification residues. The acidification and complexation includes: cooling the aqueous solution containing sodium pyridine-2-carboxylate obtained from the alkali-catalyzed hydrolysis to a temperature below 50° C.; adjusting the aqueous solution to pH 3.0-8.5 with 5-50 wt % dilute H2SO4 or 3-30 wt % dilute HCl followed by batchwise addition of CrCl3, Cr(NO3)3 or Cr2(SO4)3 for complexation at 20-70° C.Type: GrantFiled: June 25, 2019Date of Patent: July 7, 2020Assignee: YANCHENG INSTITUTE OF TECHNOLOGYInventors: Zhaosheng Cai, Jinghua Yu, Pan Xu, Jinyu Bian, Ge Ding, Lili Shen, Xujuan Huang, Zhonglie Yang, Yun Chen, Peng Xu
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Patent number: 10465172Abstract: The present invention relates to peroxygenase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.Type: GrantFiled: December 1, 2014Date of Patent: November 5, 2019Assignee: Novozymes A/SInventors: Jesper Vind, Lars Henrik Oestergaard, Leonardo DeMaria, Lisbeth Kalum, Eleni Amourgi
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Patent number: 10407702Abstract: A novel process is provided for the efficient preparation of an asymmetric compound of structural formula I: employing dynamic kinetic resolution (DKR). The DKR process involves an enzymatic enantioselective amination reaction catalyzed by transaminases. The process can be used to manufacture key intermediates in the preparation of poly (ADP-ribose) polymerase (PARP) inhibitors which may be useful for the treatment of cancer.Type: GrantFiled: August 1, 2017Date of Patent: September 10, 2019Assignee: Merck Sharp & Dohme Corp.Inventors: Paul Gerard Bulger, Birgit Kosjek, Nelo Rivera
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Patent number: 10301654Abstract: Provided is a method of preparing cinnamaldehyde by using a recombinant microorganism.Type: GrantFiled: May 13, 2016Date of Patent: May 28, 2019Assignee: INTELLIGENT SYNTHETIC BIOLOGY CENTERInventors: Ki Jun Jeong, Sun Chang Kim, Hyun Bae Bang, Yoon Hyeok Lee, Suk Chae Jung
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Patent number: 9790528Abstract: The present invention provides a method of producing lysergic acid and other ergot alkaloids by genetic modification of a fungus. A strain of fungus comprising Aspergillus fumigatus (A. fumigatus) and expressing one or more genes of the ergot alkaloid biosynthesis pathway from one or more fungus selected from the group consisting of Epichloë festucae var. lolii×Epichloë typhina isolate Lp1 (E. sp. Lp1); Claviceps species; Claviceps africana (C. africana); Claviceps gigantea (C. gigantea); Epichloë coenophiala and Periglandula species, wherein gene easA or gene easM is inactivated in said A. fumigatus, is provided.Type: GrantFiled: June 15, 2015Date of Patent: October 17, 2017Assignee: West Virginia UniversityInventors: Daniel G. Panaccione, Sarah L. Robinson
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Patent number: 9738915Abstract: A novel process is provided for the efficient preparation of an asymmetric compound of structural formula I: employing dynamic kinetic resolution (DKR). The DKR process involves an enzymatic enantioselective amination reaction catalyzed by transaminases. The process can be used to manufacture key intermediates in the preparation of poly (ADP-ribose) polymerase (PARP) inhibitors which may be useful for the treatment of cancer.Type: GrantFiled: December 3, 2013Date of Patent: August 22, 2017Assignee: Merck Sharp & Dohme Corp.Inventors: Paul Gerard Bulger, Birgit Kosjek, Nelo Rivera
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Patent number: 9611490Abstract: A method for producing cis-5-hydroxy-L-pipecolic acid is described. A gene recombinant microorganism enabling direct production of cis-5-hydroxy-L-pipecolic acid can be used in the method. Also described is a gene recombinant microorganism. In particular, it is described that a gene recombinant microorganism having DNAs encoding proteins involved in the biosynthesis of L-pipecolic acid and a DNA encoding a protein having the L-pipecolic acid cis-5-hydroxylase activity is cultured in a medium, and cis-5-hydroxy-L-pipecolic acid is collected from the medium.Type: GrantFiled: June 12, 2013Date of Patent: April 4, 2017Assignee: MicroBiopharm Japan Co., Ltd.Inventors: Tadashi Fujii, Keisuke Tamura
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Patent number: 9410169Abstract: The present invention relates to an enantioselective enzymatic process for the preparation of an optically active 3-hydroxypiperidinecarboxylic acid derivative.Type: GrantFiled: June 18, 2012Date of Patent: August 9, 2016Assignee: LABORATORIO CHIMICO INTERNAZIONALE S.P.A.Inventors: Giorgio Bertolini, Paolo Magri′
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Patent number: 9382559Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: August 10, 2012Date of Patent: July 5, 2016Assignee: Novozymes A/SInventors: Sara Landvik, Lars H. Oestergaard, Lisbeth Kalum
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Publication number: 20150148545Abstract: Disclosed in the present invention is a protein tyrosine phosphatase inhibitor. The preparation method therefor is: extracting the crude product from the Isaria Fumosorosea Wize solid or liquid fermentation broth using ethyl acetate, ethanol, methanol, or a mixed solvent of chloroform and methanol; separating the obtained extract using column chromatography on silica gel; and obtaining the target product. The inhibitor can be used to prepare pharmaceutical compositions for treating and preventing diabetes, obesity and cancers.Type: ApplicationFiled: May 7, 2013Publication date: May 28, 2015Inventors: Duqiang Luo, Jun Zhang, Zhiqin Liu
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Patent number: 9029113Abstract: Provided is a method for efficiently producing from a ketone compound an optically active amino compound useful as an intermediate of a drug, an agricultural chemical, or the like. Provided are: a polypeptide having aminotransferase activity that is increased in stereoselectivity, heat resistance, and resistance to amine compounds compared to the wild type enzyme by means of modifying an aminotransferase derived from Pseudomonas fluorescens; a gene encoding the polypeptide; and a transformant that expresses the gene at a high level.Type: GrantFiled: March 9, 2012Date of Patent: May 12, 2015Assignee: Kaneka CorporationInventors: Noriyuki Ito, Shigeru Kawano, Yoshihiko Yasohara
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Publication number: 20150118719Abstract: The present disclosure provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents. The present disclosure provides engineered proline hydroxylase biocatalysts, polynucleotides encoding the biocatalysts. methods of their preparation, and processes for preparing hydroxylated compounds using these engineered biocatalysts.Type: ApplicationFiled: May 7, 2013Publication date: April 30, 2015Inventors: Haibin Chen, Yong Koy Bong, Fabien L. Cabirol, Anupam Gohel Prafulchandra, Tao Li, Jeffrey C. Moore, Martina Quintanar-Audelo, Yang Hong, Steven J. Collier, Derek Smith
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Patent number: 8993280Abstract: The present invention provides a method for producing an alkaloid, for example, reticuline, comprising providing dopamine as a substrate for a series of actions of monoamine oxidase, norcoclaurine-6-O-methyltransferase, coclaurine-N-methyltransferase and 3?-hydroxy-N-methylcoclaurine-4?-O-methyltransferase.Type: GrantFiled: June 12, 2008Date of Patent: March 31, 2015Assignee: Kyoto UniversityInventors: Fumihiko Sato, Hiromichi Minami
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Patent number: 8975063Abstract: The present invention relates to host cells that produce compounds that are characterized as benzylisoquinolines, as well as select precursors and intermediates thereof. The host cells comprise one, two or more heterologous coding sequences wherein each of the heterologous coding sequences encodes an enzyme involved in the metabolic pathway of a benzylisoquinoline, or its precursors or intermediates from a starting compound. The invention also relates to methods of producing the benzylisoquinoline, as well as select precursors and intermediates thereof by culturing the host cells under culture conditions that promote expression of the enzymes that produce the benzylisoquinoline or precursors or intermediates thereof.Type: GrantFiled: October 19, 2007Date of Patent: March 10, 2015Assignee: California Institute of TechnologyInventors: D. Christina Smolke, Kristy Hawkins
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Publication number: 20150037850Abstract: The present invention relates to a quinolinic acid-producing recombinant microorganism expressing a fusion protein of L-aspartate oxidase and quinolinate synthase linked via a linker, and a method for producing quinolinic acid using the same.Type: ApplicationFiled: January 3, 2013Publication date: February 5, 2015Inventors: Yong Uk Shin, So Young Kim, In Kyung Heo, Ju Eun Kim, Sung Kwang Son, Jae Hee Lee, Ji Hyun Lee, Chang Gyeom Kim
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Publication number: 20150031093Abstract: To produce quinolinate effectively, a L-aspartate oxidase variant that the feedback regulation by nicotinic acid or NAD is released, and a microorganism including the L-aspartate oxidase variant are provided. Quinolinate may be effectively produced by culturing of the microorganism including the L-aspartate oxidase variant.Type: ApplicationFiled: July 25, 2014Publication date: January 29, 2015Inventors: So Young KIM, Yong Uk SHIN, In Kyung HEO, Ju Eun KIM, Kwang Ho NA, Chang Il SEO, Sung Kwang SON, Jae Hee LEE
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Patent number: 8921079Abstract: The present disclosure relates to methods of using transaminase polypeptides in the synthesis of chiral amines from prochiral ketones.Type: GrantFiled: June 21, 2010Date of Patent: December 30, 2014Assignee: Codexis, Inc.Inventors: Gregory Hughes, Paul N. Devine, Fred J. Fleitz, Brendan T. Grau, John Limanto, Christopher Savile, Emily Mundorff
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Publication number: 20140349350Abstract: The present invention relates to an improved process for the preparation of compound of Formula-II, which is an intermediate in the preparation of HMG-CoA reductase inhibitors. wherein X is hydrogen or hydroxy protecting group and R1 is carboxyl protecting group.Type: ApplicationFiled: May 28, 2014Publication date: November 27, 2014Inventors: Michiel Christian Alexander Van Vliet, Willem Robert Klass Schoevaart, Madhuresh Kumar Sethi, Sanjay Mahajan, Bhairaiah Mara
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Publication number: 20140342412Abstract: The present disclosure relates to transaminase polypeptides capable of aminating a dicarbonyl substrate, and polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides.Type: ApplicationFiled: September 7, 2012Publication date: November 20, 2014Applicant: CODEXIS, INC.Inventors: Fabien Cabirol, Haibin Chen, Anupam Gohel, Paulina Salim, Derek Smith, Jacob Janey, Birgit Kosjek, Weng Lin Tang, Helen Hsieh, Son Pham
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Patent number: 8877475Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.Type: GrantFiled: September 11, 2012Date of Patent: November 4, 2014Assignee: Codexis, Inc.Inventors: Christopher Savile, John M. Gruber, Emily Mundorff, Gjalt Huisman, Steven James Collier
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Publication number: 20140322767Abstract: The present invention relates to a droplet microreactor, i.e. a microreactor consisting of a droplet of a specific liquid, the microreactor being wall-less, wherein the interface of the specific liquid with the ambient environment and with the support on which the droplet is deposited defines the limits of the microreactor. The microreactor is characterized in that it consists of a droplet comprising at least one ionic liquid. The present invention also relates to methods for carrying out chemical or biochemical reactions and/or mixes using said droplet microreactor, and also to a lab-on-chip comprising a microreactor according to the invention.Type: ApplicationFiled: May 6, 2014Publication date: October 30, 2014Inventors: Gilles MARCHAND, Françoise VINET, Guillaume DE LAPIERRE, Fatima HASSINE, Saïd GMOUH, Michel VAULTIER
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Publication number: 20140315263Abstract: A method for the preparation of nicotinic acid, which includes the step of obtaining a culture solution containing quinolinic acid by incubating a microorganism having an ability to produce quinolinic acid, and the step of adding an acid to the culture solution and conducting a decarboxylation reaction.Type: ApplicationFiled: January 16, 2012Publication date: October 23, 2014Applicant: CJ CHEILJEDANG CORPORATIONInventors: So Young Kim, Yong Uk Shin, In Kyung Heo, Ju Eun Kim, Sung Kwang Son, Chang II Seo, Hyun Ah Kim, Han Jin Lee, Kwang Ho Na, Jee Yeon Bae
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Publication number: 20140273109Abstract: Aspects of the invention include host cells that are engineered to produce benzylisoquinoline alkaloids (BIAs). The host cells include heterologous coding sequences for a variety of enzymes involved in synthetic pathways from starting compounds to BIAs of the host cell. Also provided are methods of producing the BIAs of interest by culturing the host cells under culture conditions that promote expression of enzymes encoded by the heterologous coding sequences of the host cells. Aspects of the invention further include compositions, e.g., host cells, starting compounds and kits, etc., that find use in methods of the invention.Type: ApplicationFiled: March 14, 2014Publication date: September 18, 2014Inventors: Christina D. Smolke, Catherine Thodey, Isis Trenchard, Stephanie Galanie
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Publication number: 20140256004Abstract: The present invention relates to a novel process for the preparation of a compound of formula (I) wherein R is a methyl or methoxy group; to certain novel intermediates prepared in such a process and their use.Type: ApplicationFiled: October 19, 2012Publication date: September 11, 2014Applicant: CIPLA LIMITEDInventors: Dharmaraj Ramachandra Rao, Rajendra Narayanrao Kankan, Sanjay Naik, Maruti Ghagare, Sandip Vasant Chikhalikar
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Publication number: 20140256003Abstract: Provided are R-praziquantel preparation methods, which utilize the characteristics of biological enzyme of strong stereoselectivity, site-selectivity, and regioselectivity, of high resolution efficiency, of mild reaction conditions, and of simple operations to catalyze the hydrolysis of a certain enantiomer in a chemically synthesized racemate or a derivative, thus acquiring a mixture of reacted and unreacted optical isomers. R-praziquantel prepared by the method can have a purity of 98% or more.Type: ApplicationFiled: October 26, 2012Publication date: September 11, 2014Applicant: Suzhou Tongli Biomedical Co., Ltd.Inventor: Mingxin Qian
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Publication number: 20140120587Abstract: A method comprising the steps (a) contacting a hydrocarbon comprising a hydroxyl group with a biological agent having oxygen-dependent and cofactor-dependent carbohydrate oxidase activity in the presence of oxygen and carbohydrate oxidase cofactor, and (b) contacting the hydrocarbon produced in step a) with a biological agent having transaminase activity and a biological agent having cofactor-dependent amino acid dehydrogenase activity in the presence of amino acid dehydrogenase cofactor and the substrate amino acid of the amino acid dehydrogenase.Type: ApplicationFiled: January 10, 2012Publication date: May 1, 2014Applicant: Evonik Degussa GmbHInventors: Thomas Haas, Jan Christoph Pfeffer, Kurt Faber, Michael Fuchs
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Publication number: 20140020694Abstract: A method of modifying the content of certain chemical compounds in tobacco materials is provided, the method including treatment of a tobacco plant or portion thereof with at least one enzyme. For example, the method may modify the content of tobacco smoke toxicant precursors in tobacco materials, which can result in a modification in toxicant production when the tobacco material is exposed to elevated temperatures. The type of tobacco plant or portion thereof treated according to the invention can be, for example, a tobacco seed, a tobacco seedling, an immature live plant, a mature live plant, a harvested plant, or a plant derivative. Smoking articles and other tobacco products including such enzyme-treated tobacco materials are also provided.Type: ApplicationFiled: July 19, 2012Publication date: January 23, 2014Inventors: Serban C. Moldoveanu, Jerry Wayne Marshall, Marvin Glenn Riddick, Michael F. Davis
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Publication number: 20140011244Abstract: A method for producing naturally derived beneficial compounds including dispersing a microbiological culture media including at least one live probiotic organism, and at least one nutraceutical and/or at least one nutritive agent in distilled water to form a broth, incubating the broth at a predetermined temperature for a select period of time to induce probiotic activity; halting the probiotic activity, and separating the desired compound from the broth.Type: ApplicationFiled: September 9, 2013Publication date: January 9, 2014Applicant: Premier Research Labs, LPInventor: Robert J. Marshall
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Publication number: 20140013465Abstract: The present invention relates to genetically modified plants of the species Papaver bracteatum wherein the type or amount of one or more alkaloids produced by the plants has been modified. Specifically, the genetically modified plants have an increased expression of one or more of thebaine 6-O-demethylase, codeine O-demethylase and/or codeinone reductase relative to wild type P. bracteatum such that the genetically modified poppy plants produce an increased quantity of an alkaloid selected from codeine, oripavine and/or morphine relative to a wild type P. bracteatum. Also provided are progeny plants having the genetically modified poppy plants described above as a parent; mutant or derivative plants of the aforementioned plants; reproductive material derived from, straw produced from, straw concentrate produced from, latex derived from, or one or more isolated cells derived from, the aforementioned plants.Type: ApplicationFiled: March 15, 2013Publication date: January 9, 2014Applicant: TPI ENTERPRISES LTD.Inventors: Justin Taylor Coombs, Jarrod David Ritchie, Mark Alfred Tester, Damien Lightfoot, Deepa Jha
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Publication number: 20140004575Abstract: Provided is a method for efficiently producing from a ketone compound an optically active amino compound useful as an intermediate of a drug, an agricultural chemical, or the like. Provided are: a polypeptide having aminotransferase activity that is increased in stereoselectivity, heat resistance, and resistance to amine compounds compared to the wild type enzyme by means of modifying an aminotransferase derived from Pseudomonas fluorescens; a gene encoding the polypeptide; and a transformant that expresses the gene at a high level.Type: ApplicationFiled: March 9, 2012Publication date: January 2, 2014Applicant: Kaneka CorporationInventors: Noriyuki Ito, Shigeru Kawano, Yoshihiko Yasohara
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Patent number: 8617853Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.Type: GrantFiled: December 19, 2011Date of Patent: December 31, 2013Assignee: Codexis, Inc.Inventors: Jack Liang, Birthe Borup, Vesna Mitchell, Emily Mundorff, James Lalonde, Gjalt Huisman
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Publication number: 20130337485Abstract: The present invention discloses a process for preparing an active pharmaceutical ingredient (API) or intermediates thereof, notably particular step in the synthesis of an intermediate useful for example in the preparation of statins, by using an enzyme capable of catalyzing oxidation or dehydrogenation. The invention further provides an expression system effectively translating said enzyme. In addition, the invention relates to a specific use of such enzyme for preparing API or intermediate thereof, and in particular for preparing statin or intermediate thereof.Type: ApplicationFiled: December 20, 2011Publication date: December 19, 2013Applicant: LEK PHARMACEUTICALS D.DInventors: Peter Mrak, Tadeja Zohar, Matej Oslaj, Gregor Kopitar
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Publication number: 20130303723Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.Type: ApplicationFiled: December 28, 2012Publication date: November 14, 2013Inventors: Mark J. Burk, Anthony P. Burgard, Robin E. Osterhout, Priti Pharkya
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Publication number: 20130302859Abstract: The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.Type: ApplicationFiled: May 9, 2013Publication date: November 14, 2013Applicant: CODEXIS,INC.Inventors: CHEN Haibin, Steven J. Collier, Jovana NAZOR, Joly SUKUMARAN, Derek SMITH, Jeffrey C. MOORE, Gregory HUGHES, Jacob JANEY, Gjalt HUISMAN, Scott NOVICK, Nicholas AGARD, Oscar ALVIZO, Gregory COPE, Wan Lin YEO, Stephanie NG
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Patent number: 8551732Abstract: The present invention relates to newly identified mRNA stabilizing elements useful for the production of a target fermentation product, such as e.g. vitamins or enzymes, in particular riboflavin (vitamin B2), biotin, pantothenic acid (vitamin B5), folic acid, thiamin, pyridoxine (vitamin B6), vitamin B12, xylanase, amylase, protease, glucanase, amylomaltase or maltogenic amylase.Type: GrantFiled: June 9, 2008Date of Patent: October 8, 2013Assignee: DSM IP Assets B.V.Inventors: Martin Lehmann, Zoltan Pragai, Michéle Schaber
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Publication number: 20130260426Abstract: The present invention relates to non-naturally occurring polypeptides useful for preparing armodafinil, polynucleotides encoding the polypeptides, and methods of using the polypeptides. The non-naturally occurring polypeptides of the present invention are effective in carrying out biocatalytic conversion of the (i) 2-(benzhydrylsulfinyl)acetamide to (?)-2-[(R)-(diphenyl-methyl)sulfinyl]acetamide (armodafinil), or (ii) benzhydryl-thioacetic acid to (R)-2-(benzhydrylsulfinyl)acetic acid, which is a pivotal intermediate in the synthesis of armodafinil, in enantiomeric excess.Type: ApplicationFiled: December 7, 2011Publication date: October 3, 2013Inventors: Ee Lui Ang, Oscar Alvizo, Behnaz Behrouzian, Michael Clay, Steven Collier, Ellen Eberhard, Fan Jaslyn Fu, Shiwei Song, Derek Smith, Magnus Widegren, Robert Wilson, Junye Xu, Jun Zhu
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Publication number: 20130196013Abstract: Compositions and methods for controlling molluscs, members of the Gastropoda and Bivalvia classes which includes but is not limited to lactones, lactams, carbamates, amides, and/or carboxylic acid containing compounds as active ingredients and/or compounds derived from Pseudomonas and/or Erwinia. Also provided are methods and compositions for increasing the efficacy of chemical and biological control for invasive molluscs in open waters, power plants, and drinking water treatment facilities under coldwater conditions.Type: ApplicationFiled: March 15, 2013Publication date: August 1, 2013Applicant: MARRONE BIO INNOVATIONS, INC.Inventor: MARRONE BIO INNOVATIONS, INC.
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Publication number: 20130189731Abstract: The present invention relates to a improved process for the biotechnological production of compounds for which ribose-5-phosphate, ribulose-5-phosphate or xylulose-5-phosphate is biosynthetic precursor like riboflavin (vitamin B2), FAD, FMN, pyridoxal phosphate (vitamin B6), guanosine, GMP, adenosine, AMP. The invention further pertains to the generation of the organism producing those compounds. It furthermore relates to the generation of mutated transketolases that allow normal growth on glucose but reduced growth on gluconate when introduced into the production strains and to polynucleotides encoding them.Type: ApplicationFiled: July 26, 2012Publication date: July 25, 2013Applicant: DSM IP Assets B.V.Inventors: Michael Hans, Dietmar Laudert, Hans-Peter Hohmann, Martin Lehmann
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Publication number: 20130123563Abstract: A self-decontaminating metal organic framework including an acid linked to a metal producing a metal organic framework configured for the sorption of chemical warfare agents and/or toxic industrial chemicals, the metal organic framework including reactive sites for the degradation of the agents and chemicals.Type: ApplicationFiled: January 2, 2013Publication date: May 16, 2013Inventors: Yongwoo Lee, Thomasz Modzelewski, John P. Puglia, Steven E. Weiss, Banglin Chen
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Publication number: 20130121978Abstract: Compositions and methods for controlling molluscs, members of the Gastropoda and Bivalvia classes which includes but is not limited to lactones, lactams, carbamates, amides, and/or carboxylic acid containing compounds as active ingredients and/or compounds derived from Pseudomonas and/or Erwinia. Also provided are methods and compositions for increasing the efficacy of chemical and biological control for invasive molluscs in open waters, power plants, and drinking water treatment facilities under coldwater conditions.Type: ApplicationFiled: December 17, 2012Publication date: May 16, 2013Applicant: MARRONE BIO INNOVATIONS, INC.Inventor: Marrone Bio Innovations, Inc.
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Publication number: 20130115662Abstract: The invention relates to polypeptides having peroxygenase activity and compositions comprising such polypeptides, their encoding polynucleotides, expression vectors and recombinant host cells comprising such polynucleotides or vectors, methods of producing the polypeptides, as well as methods of application and uses thereof, including a process for enzymatic, regioselective oxygenation of N-heterocycles of the general formula (I) to the corresponding N-oxides of the formula (II), by converting N-heterocycles of the formula (I) with a peroxidase polypeptide in the presence of at least one oxidizing agent in a one-stage reaction process.Type: ApplicationFiled: December 20, 2012Publication date: May 9, 2013Applicant: NOVOZYMES A/SInventor: Novozymes A/S
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Patent number: 8367361Abstract: A method of using O6-alkylguanine-DNA alkyltransferase (AGT) is disclosed for transferring a label from a substrate to a fusion protein comprising the AGT. This allows the detection and/or manipulating of the fusion protein, both in vitro and in vivo, by attaching molecules to the fusion proteins that introduce a new physical or chemical property to the fusion protein. Examples of such molecules are, among others, spectroscopic probes or reporter molecules, affinity tags, molecules generating reactive radicals, cross-linkers, ligands mediating protein-protein interactions or molecules suitable for the immobilisation of the fusion protein.Type: GrantFiled: January 24, 2011Date of Patent: February 5, 2013Assignee: Ecole Polytechnique Federale de LausanneInventors: Kai Johnsson, Susanne Kindermann (née Gendreizig), Antje Keppler
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Patent number: 8367387Abstract: The invention relates to polypeptides having peroxygenase activity and compositions comprising such polypeptides, their encoding polynucleotides, expression vectors and recombinant host cells comprising such polynucleotides or vectors, methods of producing the polypeptides, as well as methods of application and uses thereof, including a process for enzymatic, regioselective oxygenation of N-heterocycles of the general formula (I) to the corresponding N-oxides of the formula (II), by converting N-heterocycles of the formula (I) with a peroxidase polypeptide in the presence of at least one oxidizing agent in a one-stage reaction process.Type: GrantFiled: March 31, 2008Date of Patent: February 5, 2013Assignee: Novozymes A/SInventors: Marek Jan Pecyna, Kirk Matthew Schnorr, René Ullrich, Katrin Scheibner, Martin Gunter Kluge, Martin Hofrichter
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Patent number: 8349593Abstract: Disclosed is a novel process for producing a compound represented by the general formula (1) by using a microorganism, wherein the compound is useful as a plant growth stimulant. Specifically disclosed is a process for producing a succinic acid amide compound represented by the general formula (1) [wherein Ar represents a phenyl, naphthyl or indolyl group which may have a substituent; and n represents a numeral value ranging from 1 to 4] or a salt thereof, which is characterized by culturing a microorganism of the genus Pseudomonas that is capable of synthesizing a succinic acid amide in a culture medium containing an arylalkylamine represented by the general formula (2).Type: GrantFiled: October 24, 2008Date of Patent: January 8, 2013Assignee: Sumitomo Chemical Company, LimitedInventors: Toru Kitamura, Hiroshi Soejima, Tamizi Sugiyama
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Patent number: 8338142Abstract: The present invention relates to a method for producing an optically active 3-aminopiperidine or salt thereof. In the method, a racemic nipecotamide is stereoselectively hydrolyzed to obtain an optically active nipecotamide and an optically active nipecotic acid in the presence of an enzyme source derived from an organism, and then the optically active nipecotamide is derived into an optically active aminopiperidine or salt thereof by aroylation, Hofmann rearrangement, deprotection of the amino group and further deprotection; or the optically active nipecotamide is derived into an optically active aminopiperidine or salt thereof by selective protection with BOC, Hofmann rearrangement and further deprotection. It is possible by the present invention to produce an optically active 3-aminopiperidine or salt thereof useful as a pharmaceutical intermediate from an inexpensive and easily available starting material by easy method applicable to industrial manufacturing.Type: GrantFiled: February 18, 2008Date of Patent: December 25, 2012Assignee: Kaneka CorporationInventors: Kohei Mori, Masutoshi Nojiri, Akira Nishiyama, Naoaki Taoka
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Patent number: 8288141Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.Type: GrantFiled: August 27, 2009Date of Patent: October 16, 2012Assignee: Codexis, Inc.Inventors: Christopher Savile, John M. Gruber, Emily Mundorff, Gjalt Huisman, Steven James Collier
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Patent number: 8288132Abstract: The present invention discloses a chemoenzymatic process for the preparation of an iminocyclitol corresponding to formula (I), (II), (III) or (IV), wherein: R1 and R2 are the same or different, and independently selected from the group consisting of: H, OH, hydroxymethyl, methyl, ethyl, butyl, pentyl, hexyl, octyl, isopropyl, isobutyl, 2-methylbutyl, and benzyl; R3 is selected from the group consisting of: H, hydroxymethyl, hydroxyethyl, ethyl, butyl, pentyl, hexyl, octyl, dodecyl, isobutyl, isopropyl, isopentyl, 2-methylbutyl, benzyl, and phenylethyl; n: 0 or 1; the process comprising: (i) an aldol addition catalyzed by a D-fructose-6-phosphate aldolase enzyme (FSA) and an acceptor aminoaldehyde; and (ii) an intramolecular reductive amination of the addition adduct obtained in step (i) with H2, in the presence of a metallic catalyst, optionally being carried out said step (ii) in the presence of an aldehyde of formula R3—CHO, wherein R3 is as defined above.Type: GrantFiled: August 30, 2007Date of Patent: October 16, 2012Assignees: Consejo Superior de Investigaciones Cientificas, Bioglane, S.L.N.E.Inventors: Pere Clapés Saborit, Jesús Joglar Tamargo, José Antonio Castillo Expósito, Carles Lozano Pérez
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Publication number: 20120196333Abstract: The process for the synthesis of statins featuring the use of an early intermediate (4R,6S)-6-(dialkoxymethyl)tetrahydro-2H-pyran-2,4-diol which already possesses the desired stereochemistry corresponding to the final statin.Type: ApplicationFiled: March 12, 2012Publication date: August 2, 2012Applicant: LEK PHARMACEUTICALS D.D.Inventors: ZDENKO CASAR, TOMAZ MESAR, GREGOR KOPITAR, PETER MRAK, MATEJ OSLAJ
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Publication number: 20120190085Abstract: The present disclosure relates to methods of using transaminase polypeptides in the synthesis of chiral amines from prochiral ketones.Type: ApplicationFiled: June 21, 2010Publication date: July 26, 2012Applicant: CODEXIS, INC.Inventors: Gregory Hughes, Paul N. Devine, Fred J. Fleitz, Brendan T. Grau, John Limanto, Christopher Savile, Emily Mundorff