Amide (e.g., Chloramphenicol, Etc.) Patents (Class 435/129)
  • Patent number: 11021724
    Abstract: The subject invention concerns materials and methods for alkene reduction of compounds, such as levoglucosenone (LGO) and (S)-?-hydroxymethyl-?,?-butenolide (HBO), using an alkene reductase enzyme. In one embodiment, a method of the invention comprises alkene reduction of a target compound by reacting the compound with an Old Yellow Enzyme (OYE) that reduces alkene bonds. In one embodiment, the OYE is OYE 2.6 from Pichia stipites and comprises the amino acid sequence of SEQ ID NO: I. In a specific embodiment, the enzyme is an Old Yellow Enzyme (OYE) 2.6 mutant having an amino acid substitution at position 78 in the sequence, wherein the tyrosine at position 78 is substituted with a tryptophan amino acid (Y78W) and is designated as OYE 2.6 Y78W (SEQ ID NO:2).
    Type: Grant
    Filed: March 29, 2018
    Date of Patent: June 1, 2021
    Assignees: University of Florida Research Foundation, Inc., AfroParisTech
    Inventors: Jon Dale Stewart, Florent Allais, Louis Michel Marie Mouterde
  • Patent number: 10640798
    Abstract: The present invention relates to a microorganism for producing diamine, in which activity of a protein having an amino acid sequence of SEQ ID NO: 6 or an amino acid sequence having 42% or higher sequence homology with SEQ ID NO: 6 is introduced or enhanced, and a method of producing diamine using the same.
    Type: Grant
    Filed: June 5, 2018
    Date of Patent: May 5, 2020
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Kyoung Min Lee, Su Jin Park, Hee Kyoung Jung, Young Lyeol Yang, Hong Xian Li, Hye Won Um
  • Patent number: 10358651
    Abstract: The present invention provides for novel compositions and methods of producing modified lignin by modulating expression of acyltransferases.
    Type: Grant
    Filed: February 29, 2016
    Date of Patent: July 23, 2019
    Assignee: The Regents of the University of California
    Inventors: Dominique Loque, Aymerick Guillaume Eudes
  • Patent number: 10144952
    Abstract: The present invention provides methods, compositions and devices for stabilizing the extracellular nucleic acid population in a cell-containing biological sample using butanamide.
    Type: Grant
    Filed: March 18, 2014
    Date of Patent: December 4, 2018
    Assignee: QIAGEN GmbH
    Inventors: Ralf Wyrich, Thorsten Voss, Daniel Grölz
  • Patent number: 10136666
    Abstract: The present invention relates to flavor generation. In particular the invention relates to a process for the preparation of a flavor composition. The process comprises forming a deep eutectic solvent, preparing a reaction mixture comprising the deep eutectic solvent and flavor precursors, and heating the reaction mixture to form aroma compounds. The deep eutectic solvent is a liquid based on a combination of at least two compounds solid at 25° C. and comprises water and/or glycerol in an amount insufficient to dissolve all the compounds solid at 25° C. individually, or in an amount such that all the compounds solid at 25° C. are simultaneously saturated at 25° C. The flavor precursors may comprise the compounds solid at 25° C. on which the deep eutectic solvent is based. A further aspect of the invention is a food product comprising the flavor composition obtainable by the process of the invention.
    Type: Grant
    Filed: September 23, 2014
    Date of Patent: November 27, 2018
    Assignee: Nestec S.A.
    Inventors: Heiko Oertling, Florian Viton, Candice Marie Menozzi, Laurent Forny, Walter Matthey-Doret
  • Patent number: 9951358
    Abstract: A biosynthetic method of making a capsaicinoid including expressing a first gene product of CS/AT3/Pun1 in a cellular system, growing the cellular system in a medium, and collecting the capsaicinoid. The biosynthetic method further includes expressing a second gene product of ACS1 and expressing a third gene product of pAMT in the cellular system.
    Type: Grant
    Filed: January 16, 2015
    Date of Patent: April 24, 2018
    Assignee: Conagen Inc.
    Inventors: Hui Chen, Hongxue Wang, Oliver Yu
  • Patent number: 9951319
    Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: May 4, 2016
    Date of Patent: April 24, 2018
    Assignee: Novozymes A/S
    Inventors: Sara Landvik, Lars H. Oestergaard, Lisbeth Kalum
  • Patent number: 9879290
    Abstract: Compositions and methods for a hybrid biological and chemical process utilizing chemotrophic microorganisms that converts syngas and/or gaseous CO2 and/or a mixture of CO2 gas and H2 gas into one or more desaturated hydrocarbons, unsaturated fatty acids, hydroxy acids, or diacids.
    Type: Grant
    Filed: September 19, 2012
    Date of Patent: January 30, 2018
    Assignee: KIVERDI, INC.
    Inventors: Itzhak Kurek, Michael Siani-Rose, Lisa Dyson, Christer Jannson, John S. Reed
  • Patent number: 9493744
    Abstract: The invention provides for methods of viral inactivation using high temperature short time (HTST) treatment and adjustment of various parameters such that generation of precipitate and depositions of precipitate are reduced and/or minimized.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: November 15, 2016
    Assignee: GENENTECH, INC.
    Inventors: Masaru Ken Shiratori, Robert David Kiss, Hardayal Prashad, Raquel Iverson, Justin Bourret, Michael Kim, Salim Charaniya
  • Patent number: 9458478
    Abstract: The invention relates to enzymatic methods for epoxidation of a non-cyclicaliphatic alken, or a terpen.
    Type: Grant
    Filed: March 25, 2013
    Date of Patent: October 4, 2016
    Assignee: Novozymes A/S
    Inventors: Henrik Lund, Lisbeth Kalum, Martin Hofrichter, Sebastian Peter
  • Patent number: 9388403
    Abstract: Improving wild nitrile hydratase enables the provision of a protein which has nitrile hydratase activity and which has further improved heat resistance, amide compound resistance and high temperature accumulation properties. Use protein (A) or (B), (A) being a protein characterized by having nitrile hydratase activity and by including an amino acid sequence in which a specific amino acid residue in an amino acid sequence in wild nitrile hydratase has been substituted by another amino acid residue, and (B) being a protein characterized by having nitrile hydratase activity and by including an amino acid sequence in which one or several amino acid residues in the amino acid sequence of protein (A), other than the abovementioned specific amino acid residue, is deleted, substituted and/or added.
    Type: Grant
    Filed: May 30, 2012
    Date of Patent: July 12, 2016
    Assignee: Mitsubishi Rayon Co., Ltd.
    Inventors: Fumiaki Watanabe, Takanori Ambo, Ai Hara
  • Patent number: 9376694
    Abstract: The present disclosure relates to a method for preparing an optically active amino acid using cosubstrate shuttling of transaminase. The method includes coupling a reaction of converting a keto acid to an amino acid by ?-transaminase and a reaction of transferring an amino group of an amine substrate by ?-transaminase (TA) using an amino acid cosubstrate. The present disclosure allows production of various optically active amino acids with high purity and high efficiency by solving the low equilibrium constant problem of transaminase and is applicable to production of various optically active amino acids in industrial scale. Since the present disclosure allows easy production of various unnatural amino acids having high reactivity and stability, which are used as pharmaceutical precursors, it can be usefully employed in preparation of pharmaceuticals, food additives and various animal feeds.
    Type: Grant
    Filed: July 8, 2013
    Date of Patent: June 28, 2016
    Assignee: INDUSTRY-ACADEMIC COOPERATION FOUNDATION, YONSEI UNIVERSITY
    Inventors: Jong-Shik Shin, Eul-Soo Park
  • Patent number: 9181569
    Abstract: The present invention relates to a method for producing acrylamide comprising: supplying a raw material water to a reactor, supplying acrylonitrile to the reactor, and hydrating acrylonitrile using a biocatalyst, wherein a temperature of the raw material water in the supplying step of the raw material water to the reactor is equal to or more than freezing point of the raw material water and lower than the reaction temperature by 5° C. or more. The present invention can be provided to the method of producing acrylamide which can be removed the reaction heat produced at the hydration reaction of acrylonitrile effectively with a low cost.
    Type: Grant
    Filed: May 29, 2012
    Date of Patent: November 10, 2015
    Assignee: Mitsubishi Rayon co., Ltd.
    Inventors: Makoto Kanou, Kiyonobu Niwa
  • Patent number: 9139819
    Abstract: The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.
    Type: Grant
    Filed: November 14, 2012
    Date of Patent: September 22, 2015
    Assignee: Codexis, Inc.
    Inventors: Fabien Louis Cabirol, Haibin Chen, Anupam Gohel, Steven J. Collier, Derek J. Smith, Birgit Kosjek, Jacob Janey
  • Patent number: 9102590
    Abstract: There is provided a method for producing an aqueous acrylamide solution by producing acrylamide by reacting a composition including acrylonitrile with water, in which the composition including acrylonitrile includes 20 to 80 mg of methacrylonitrile per 1 kg of the total weight of the composition including acrylonitrile. According to the present invention, a production method allowing stable obtainment of an aqueous acrylamide solution can be provided as polymerization of the acrylamide can be suppressed without causing a decreas+455e in quality.
    Type: Grant
    Filed: May 21, 2012
    Date of Patent: August 11, 2015
    Assignee: Mitsubishi Rayon Co., Ltd.
    Inventors: Makoto Kanou, Norifumi Hagiya
  • Patent number: 9057084
    Abstract: There is provided a method for producing an aqueous acrylamide solution by reacting a composition including acrylonitrile with water to produce acrylamide, in which the composition including acrylonitrile includes 3 to 15 mg of propionitrile per 1 kg of the total weight of the composition including acrylonitrile. According to the present invention, a production method with which it is possible to suppress acrylamide polymerization without lowering quality and thereby obtain a stable aqueous acrylamide solution can be provided.
    Type: Grant
    Filed: May 21, 2012
    Date of Patent: June 16, 2015
    Assignee: Mitsubishi Rayon Co., Ltd.
    Inventors: Makoto Kanou, Norifumi Hagiya
  • Publication number: 20150118721
    Abstract: The invention relates to novel enzymes that provide acetylated sphingoid bases.
    Type: Application
    Filed: March 13, 2013
    Publication date: April 30, 2015
    Applicant: EVONIK INDUSTRIES AG
    Inventors: Steffen Schaffer, Mike Farwick, Heiko Andrea, Tim Koehler, Daniel Wolff, Frank ter Veld, Ansgar Poetsch, Eckhard Boles, Christoph Schorsch
  • Publication number: 20150079643
    Abstract: The disclosure relates to a recombinant microorganism engineered to express an enzyme which catalyzes the conversion of a primary amine and an acyl thioester to a fatty amide. The disclosure further encompasses a method of producing a fatty amide by culturing the recombinant microorganism in the presence of a carbon source.
    Type: Application
    Filed: March 12, 2013
    Publication date: March 19, 2015
    Applicant: REG Life Sciences, LLC.
    Inventors: Jason J. Lutes, Stephen del Cardayre
  • Publication number: 20150079644
    Abstract: The present invention provides a method for conveniently and efficiently producing a 3-acetylamino-4-hydroxybenzoic acid-type compound that is a stable compound by a process using a microorganism. Specifically the present invention provides a microorganism having an ability to produce 3-amino-4-hydroxybenzoic acid, that is modified so as to increase an activity to form 3-amino-4-hydroxybenzoic acid from dihydroxyacetone phosphate and aspartate semialdehyde, wherein the microorganism is modified so as to increase an N-hydroxyarylamine O-acetyltransferase (NhoA) activity, as well as a method for producing the 3-acetylamino-4-hydroxybenzoic acid-type compound using such a microorganism.
    Type: Application
    Filed: November 19, 2014
    Publication date: March 19, 2015
    Applicant: AJINOMOTO CO., INC.
    Inventors: Fumi Shozui, Yoshinori Tajima, Keiichi Yokoyama
  • Patent number: 8980588
    Abstract: A method for producing acrylamide from acrylonitrile by a biocatalyst method, wherein both evaporation of acrylonitrile into a gas phase and damaging of a catalyst by stirring are prevented, is provided. In the present invention, the production of acrylamide from acrylonitrile by the biocatalyst method comprises feeding acrylonitrile into an aqueous medium comprising a microbial catalyst.
    Type: Grant
    Filed: December 21, 2010
    Date of Patent: March 17, 2015
    Assignee: Mitsubishi Rayon Co., Ltd.
    Inventors: Takamitsu Kariya, Hiroyasu Banba, Makoto Kano
  • Patent number: 8980578
    Abstract: The present invention provides fungal xylanase and/or beta-xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase and/or beta-xylosidases, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.
    Type: Grant
    Filed: June 10, 2013
    Date of Patent: March 17, 2015
    Assignee: Codexis, Inc.
    Inventors: Ryan Fong, Xiyun Zhang, Chris Noriega, Nicholas Agard, Anupam Gohel, Derek Smith
  • Publication number: 20150057465
    Abstract: The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.
    Type: Application
    Filed: March 17, 2014
    Publication date: February 26, 2015
    Inventors: Hans LIAO, Christopher Patrick MERCOGLIANO, Travis Robert WOLTER, Michael Tai Man LOUIE, Wendy Kathleen RIBBLE, Tanya LIPSCOMB, Eileen Colie SPINDLER, Michael D. LYNCH
  • Publication number: 20150056669
    Abstract: The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.
    Type: Application
    Filed: March 17, 2014
    Publication date: February 26, 2015
    Inventors: Hans LIAO, Christopher Patrick MERCOGLIANO, Travis Robert WOLTER, Michael Tai Man LOUIE, Wendy Kathleen RIBBLE, Tanya LIPSCOMB, Eileen Colie SPINDLER, Michael D LYNCH
  • Patent number: 8940934
    Abstract: An object of the present invention is to provide a production process of an ?-hydroxy acid having a sufficient quality as a polymer raw material, which process does not produce a large amount of waste as a byproduct and is economical. The present invention provides a production process of an ?-hydroxy acid having a step of adding a basic metal to an ?-hydroxy acid ammonium salt to yield an ?-hydroxy acid metal salt and a step of desalting the ?-hydroxy acid metal salt to yield the ?-hydroxy acid.
    Type: Grant
    Filed: June 20, 2008
    Date of Patent: January 27, 2015
    Assignee: Asahi Kasei Chemicals Corporation
    Inventors: Makoto Okamoto, Hideki Date
  • Publication number: 20140377815
    Abstract: The present invention relates to a method of producing carbamoyl phosphate, the method comprising reacting ammonia, ATP, bicarbonate and CO2, or a hydrated form thereof, in a composition in the presence of a carbamate kinase, wherein the ammonia and CO2, or hydrated form thereof, are converted to carbamate in a chemical reaction and the carbamate and ATP are converted to carbamoyl phosphate in an enzyme-catalysed reaction by the carbamate kinase, and wherein the pH of the composition is about 8 to about 12. The invention also relates to methods of producing urea.
    Type: Application
    Filed: June 15, 2012
    Publication date: December 25, 2014
    Inventors: James Edward Hennessy, Amy Philbrook, Daniel Miles Bartkus, Christopher John Easton, Colin Scott, John G. Oakeshott, Hye-Kyung Kim, Melissa Jane Latter
  • Publication number: 20140377291
    Abstract: Provided herein are sphingolipid compounds that are useful for activating natural killer T cells. Also provided are methods for treating or preventing a disease or disorder that is treatable by activating the immune system by stimulating natural killer T cells. The compounds are therefore useful for treating or reducing the likelihood of occurrence of an immune diseases and disorders, such as autoimmune diseases or disorders. The compounds may also be used for treating or reducing the likelihood of occurrence of a microbial infection or for treating or reducing the likelihood of occurrence of a cancer in a subject by administering the sphingolipid compounds described herein.
    Type: Application
    Filed: February 7, 2013
    Publication date: December 25, 2014
    Inventors: Michael A. Fischbach, Jeffrey A. Bluestone, Cristina Penaranda, Laura Brown, Jon Clardy
  • Publication number: 20140349350
    Abstract: The present invention relates to an improved process for the preparation of compound of Formula-II, which is an intermediate in the preparation of HMG-CoA reductase inhibitors. wherein X is hydrogen or hydroxy protecting group and R1 is carboxyl protecting group.
    Type: Application
    Filed: May 28, 2014
    Publication date: November 27, 2014
    Inventors: Michiel Christian Alexander Van Vliet, Willem Robert Klass Schoevaart, Madhuresh Kumar Sethi, Sanjay Mahajan, Bhairaiah Mara
  • Patent number: 8889907
    Abstract: The present invention provides a method for producing an amide compound from a nitrile compound using a biocatalyst that realizes low cost, energy saving and low environmental burdens. The production method of the amide compound of the present invention is a method for producing an amide compound from a nitrile compound using a biocatalyst in a reactor, wherein the nitrile compound is reacted with the biocatalyst to produce the amide compound under such stirring conditions that the stirring power requirement is in the range of 0.08 to 0.7 kW/m3.
    Type: Grant
    Filed: March 13, 2009
    Date of Patent: November 18, 2014
    Assignee: Mitsubishi Rayon Co., Ltd.
    Inventors: Makoto Kanou, Kiyonobu Niwa, Masahito Oda
  • Patent number: 8889380
    Abstract: The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
    Type: Grant
    Filed: September 5, 2012
    Date of Patent: November 18, 2014
    Assignee: Codexis, Inc.
    Inventors: Christopher Savile, Emily Mundorff, Jeffrey Moore, Paul N. Devine, Jacob M. Janey
  • Publication number: 20140335575
    Abstract: An object of the present invention is to provide a means for producing an optically active tropic acid that is a compound useful as a synthetic raw material or an intermediate for pharmaceutical products and the like. The present invention provides a novel polypeptide having activity to (R)-selectively hydrolyze a racemic tropic acid amide, DNA encoding the polypeptide, a vector containing the DNA, a transformant prepared by transformation with the vector, and a method for producing an optically active carboxylic acid amide and an optically active carboxylic acid using them.
    Type: Application
    Filed: September 28, 2012
    Publication date: November 13, 2014
    Inventors: Masutoshi Nojiri, Hiroyuki Kanamaru, Akiko Nishi, Shigeru Kawano, Yoshihiko Yasohara
  • Publication number: 20140329916
    Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.
    Type: Application
    Filed: December 16, 2013
    Publication date: November 6, 2014
    Applicant: Genomatica, Inc.
    Inventors: Anthony P. Burgard, Robin E. Osterhout, Stephen J. Van Dien, Cara Ann Tracewell, Priti Pharkya, Stefan Andrae
  • Publication number: 20140322769
    Abstract: The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.
    Type: Application
    Filed: November 14, 2012
    Publication date: October 30, 2014
    Inventors: Fabien Louis Cabirol, Haibin Chen, Anupam Gohel, Steven J. Collier, Derek J. Smith, Birgit Kosjek, Jacob Janey
  • Publication number: 20140322770
    Abstract: The present invention relates to isolated polypeptides having peroxygenaseactivity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: November 28, 2012
    Publication date: October 30, 2014
    Inventors: Sara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
  • Publication number: 20140273110
    Abstract: Bacteria that run the beta oxidation cycle in reverse anabolic direction are provided, along with many novel primers to start the reverse cycle, pathways to make such primers, and a large variety of products produced thereby. Methods for making desired product by using such primers in the reverse pathway are also disclosed.
    Type: Application
    Filed: March 6, 2014
    Publication date: September 18, 2014
    Applicant: William Marsh Rice University
    Inventors: Ramon GONZALEZ, James M. CLOMBURG
  • Publication number: 20140256005
    Abstract: The present invention relates to a novel method for the fermentative production of gamma-aminobutyric acid (GABA) by cultivating a recombinant microorganism expressing an enzyme having a glutamate decarboxylase activity. The present invention also relates to corresponding recombinant hosts, recombinant vectors, expression cassettes and nucleic acids suitable for preparing such hosts as well as to a method for preparing polyamides making use of GABA as obtained fermentative production.
    Type: Application
    Filed: May 29, 2014
    Publication date: September 11, 2014
    Applicant: BASF SE
    Inventors: Oskar Zelder, Weol Kyu Jeong, Corinna Klopprogge, Andrea Herold, Hartwig Schröder
  • Publication number: 20140256904
    Abstract: The subject of the invention is a biotechnological process for the production of ?-functionalized carboxylic acids and of ?-functionalized carboxylate esters from simple carbon sources.
    Type: Application
    Filed: August 15, 2012
    Publication date: September 11, 2014
    Applicant: Evonik Degussa GmbH
    Inventors: Steffen Schaffer, Nicole Decker, Jasmin Gielen, Harald Haeger, Thomas Haas, Markus Poetter, Hans-Georg Hennemann, Mirja Wessel, Michael Volland
  • Publication number: 20140242647
    Abstract: The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.
    Type: Application
    Filed: February 20, 2014
    Publication date: August 28, 2014
    Applicant: California Institute of Technology
    Inventors: Pedro S. Coelho, Eric M. Brustad, Frances H. Arnold, Zhan Wang, Jared C. Lewis
  • Publication number: 20140234917
    Abstract: The invention relates to enzymatic methods for hydroxylation in position 2 or 3 of two ends of a substituted or unsubstituted, linear or branched aliphatic hydrocarbons.
    Type: Application
    Filed: June 29, 2012
    Publication date: August 21, 2014
    Applicant: Novozymes A/S
    Inventors: Henrik Lund, Jesper Brask, Lisbeth Kalum, Ana Gutierrez Suarez, Esteban Daniel Babot, Rene Ullrich, Martin Hofrichter, Angel Tomas Martinez Ferrer, Jose Carlos Del Rio Andrade
  • Publication number: 20140234918
    Abstract: The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Application
    Filed: August 10, 2012
    Publication date: August 21, 2014
    Applicant: Novozymes A/S
    Inventors: Sara Landvik, Lars Henrik Oestergaard, Lisbeth Kalum
  • Publication number: 20140220644
    Abstract: Provided is an improved nitrile hydratase with improved catalytic activity. Also provided are DNA for coding the improved nitrile hydratase, a recombinant vector that contains the DNA, a transformant that contains the recombinant vector, nitrile hydratase acquired from a culture of the transformant, and a method for producing the nitrile hydratase. Also provided is a method for producing an amide compound that uses the culture or a processed product of the culture. The improved nitrile hydratase contains an amino acid sequence represented by SEQ ID NO: 50 (GX1X2X3X4DX5X6R) in a beta subunit, and is characterized in that X4 is an amino acid selected from a group comprising cysteine, aspartic acid, glutamic acid, histidine, isoleucine, lysine, methionine, asparagine, proline, glutamine, serine and threonine.
    Type: Application
    Filed: June 7, 2012
    Publication date: August 7, 2014
    Applicant: Mitsubishi Rayon Co., Ltd
    Inventors: Fumiaki Watanabe, Ai Hara, Takanori Ambo, Aya Kitahara
  • Publication number: 20140199737
    Abstract: This document describes biochemical pathways for producing adipic acid, 6-aminohexanoic acid, 6-hydroxhexanoic acid, hexamethylenediamine, caprolactam, or 1,6-hexanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C6 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the enzymes or homologs accepting methyl ester shielded dicarboxylic acid substrates.
    Type: Application
    Filed: December 23, 2013
    Publication date: July 17, 2014
    Inventors: Adriana Leonora Botes, Alex Van Eck Conradie
  • Publication number: 20140187818
    Abstract: The invention relates to a bacterial strain belonging to the genus Rhodococcus which is a producer of a nitrile hydratase. The invention also relates to a method for producing acrylamide by hydration of acrylonitrile using a biomass of the bacterial strain and to a method of culturing the bacterial strain.
    Type: Application
    Filed: December 24, 2013
    Publication date: July 3, 2014
    Inventors: Sergey Vladimirovich Kozulin, Tatiana Nicolaevna Kozulina, Alexey Sergeevich Kozulin
  • Patent number: 8748661
    Abstract: The present invention relates to novel polyamino polyketide antibiotics, methods of their production as well as methods of using these antibiotics, for example, for inhibition or removal of biofilm formation or for treating bacterial infection with these antibiotics.
    Type: Grant
    Filed: November 9, 2009
    Date of Patent: June 10, 2014
    Assignee: Agency for Science, Technology and Research
    Inventors: Jien Wu, Haibao Zhang, Lianhui Zhang
  • Publication number: 20140134682
    Abstract: Recombinant microorganisms comprising DNA molecules in a deregulated form which improve the production of cadaverine or N-acetylcadaverine, as well as recombinant DNA molecules and polypeptides used to produce the microorganisms are provided. Said microorganisms comprise an intracellular lysine decarboxylase activity and a deregulated cadaverine export activity, or comprise a decreased cadaverine export activity and an enhanced N-acetylcadaverine forming activity. Processes for the production of cadaverine N-acetylcadaverine using the recombinant microorganisms are also provided.
    Type: Application
    Filed: February 20, 2012
    Publication date: May 15, 2014
    Applicant: BASF SE
    Inventors: Christoph Wittmann, Stefanie Kind
  • Publication number: 20140134683
    Abstract: The present invention relates to a method for producing acrylamide comprising: supplying a raw material water to a reactor, supplying acrylonitrile to the reactor, and hydrating acrylonitrile using a biocatalyst, wherein a temperature of the raw material water in the supplying step of the raw material water to the reactor is equal to or more than freezing point of the raw material water and lower than the reaction temperature by 5° C. or more. The present invention can be provided to the method of producing acrylamide which can be removed the reaction heat produced at the hydration reaction of acrylonitrile effectively with a low cost.
    Type: Application
    Filed: May 29, 2012
    Publication date: May 15, 2014
    Applicant: Mitsubishi Rayon Co., Ltd.
    Inventors: Makoto Kanou, Kiyonobu Niwa
  • Publication number: 20140120588
    Abstract: Improving wild nitrile hydratase enables the provision of a protein which has nitrile hydratase activity and which has further improved heat resistance, amide compound resistance and high temperature accumulation properties. Use protein (A) or (B), (A) being a protein characterised by having nitrile hydratase activity and by including an amino acid sequence in which a specific amino acid residue in an amino acid sequence in wild nitrile hydratase has been substituted by another amino acid residue, and (B) being a protein characterised by having nitrile hydratase activity and by including an amino acid sequence in which one or several amino acid residues in the amino acid sequence of protein (A), other than the abovementioned specific amino acid residue, is deleted, substituted and/or added.
    Type: Application
    Filed: May 30, 2012
    Publication date: May 1, 2014
    Applicant: Mitsubishi Rayon Co., Ltd.
    Inventors: Fumiaki Watanabe, Takanori Ambo, Ai Hara
  • Publication number: 20140106415
    Abstract: There is provided a method for producing an aqueous acrylamide solution by producing acrylamide by reacting a composition including acrylonitrile with water, in which the composition including acrylonitrile includes 20 to 80 mg of methacrylonitrile per 1 kg of the total weight of the composition including acrylonitrile. According to the present invention, a production method allowing stable obtainment of an aqueous acrylamide solution can be provided as polymerization of the acrylamide can be suppressed without causing a decreas+455e in quality.
    Type: Application
    Filed: May 21, 2012
    Publication date: April 17, 2014
    Applicant: Mitsubishi Rayon Co., Ltd.
    Inventors: Makoto Kanou, Norifumi Hagiya
  • Publication number: 20140087434
    Abstract: There is provided a method for producing an aqueous acrylamide solution by reacting a composition including acrylonitrile with water to produce acrylamide, in which the composition including acrylonitrile includes 3 to 15 mg of propionitrile per 1 kg of the total weight of the composition including acrylonitrile. According to the present invention, a production method with which it is possible to suppress acrylamide polymerization without lowering quality and thereby obtain a stable aqueous acrylamide solution can be provided.
    Type: Application
    Filed: May 21, 2012
    Publication date: March 27, 2014
    Applicant: Mitsubishi Rayon Co., Ltd.
    Inventors: Makoto Kanou, Norifumi Hagiya
  • Publication number: 20140051133
    Abstract: Provided herein are polypeptides having ketol-aid reductoisomerase activity as well as microbial host cells comprising such polypeptides. Polypeptides provided herein may be used in biosynthetic pathways, including, but not limited to, isobutanol biosynthetic pathways.
    Type: Application
    Filed: May 10, 2013
    Publication date: February 20, 2014
    Applicant: BUTAMAX ADVANCED BIOFUELS, LLC
    Inventors: Sridhar Govindarajan, Yougen Li, Der-Ing Liao, Daniel P. O'Keefe, Jeremy Stephen Minshull, Steven Cary Rothman, Alexander Vincent Tobias
  • Patent number: 8647848
    Abstract: The invention relates to the enzymatic synthesis of sphingolipids and compositions that contain sphingolipids from lysosphingolipids and carbonic esters, and to cosmetic, dermatological or pharmaceutical formulations containing said sphingolipids or compositions.
    Type: Grant
    Filed: May 22, 2009
    Date of Patent: February 11, 2014
    Assignee: Evonik Goldschmidt GmbH
    Inventors: Frank Hollmann, Oliver Thum, Christoph Toelle, Angelo Provinzano, Cornelis Gerrit Nijs Korevaar