Preparing Nitrogen-containing Organic Compound Patents (Class 435/128)
  • Patent number: 10227621
    Abstract: The present invention relates to methods for preparing an aqueous acrylamide solution having a low acrylic acid concentration. In addition, the present invention relates to methods for reducing the acrylic acid concentration of an aqueous acrylamide solution. The methods involve a bioconversion of acrylonitrile to acrylamide in the presence of a biocatalyst, wherein during the bioconversion the content of acrylonitrile is maintained at 0.3 w/w % or more referred to the total weight of the composition in the reactor. Also provided is an aqueous acrylamide solution which is obtained by the methods of the present invention. Furthermore, the present invention is related to an acrylamide homopolymer or copolymer obtained by polymerizing the acrylamide of the aqueous solution.
    Type: Grant
    Filed: September 30, 2015
    Date of Patent: March 12, 2019
    Assignee: BASF SE
    Inventors: Michael Guenter Braun, Juergen Daeuwel, Hans-Juergen Lang, Peter Oedman, Kai-Uwe Baldenius, Matthias Kleiner, Michael Kiefer, Stephan Freyer, Michael Budde
  • Patent number: 10221433
    Abstract: The present invention relates to a recombinant microorganism capable of producing putrescine, in which the microorganism is modified to have enhanced NCgl2522 activity, thereby producing putrescine in a high yield, and a method for producing putrescine using the microorganism.
    Type: Grant
    Filed: February 25, 2014
    Date of Patent: March 5, 2019
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Kyoung Min Lee, Hee Kyoung Jung, Young Lyeol Yang, Hye Won Um, Chang Gyeom Kim, Hong Xian Li, Su Jin Park, Jong Hyun Yoon, Baek Seok Lee, Sun Young Lee
  • Patent number: 10196667
    Abstract: The present disclosure relates to the use of an amino acid dehydrogenase in combination with a cofactor regenerating system comprising a ketoreductase. In particular embodiments, the process can be used to prepare L-tert-leucine using a leucine dehydrogenase.
    Type: Grant
    Filed: June 20, 2017
    Date of Patent: February 5, 2019
    Assignee: Codexis, Inc.
    Inventors: Fabien Louis Cabirol, Steven J. Collier, Thomas Daussmann, Naga K. Modukuru
  • Patent number: 10160985
    Abstract: The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
    Type: Grant
    Filed: March 8, 2018
    Date of Patent: December 25, 2018
    Assignee: Codexis, Inc.
    Inventors: Christopher K. Savile, Emily Mundorff, Jeffrey C. Moore, Paul N. Devine, Jacob M. Janey
  • Patent number: 10072275
    Abstract: A method of producing 2,3-butanediol includes the steps of: culturing a microorganism belonging to the genus Zymobacter in a fermentation feedstock containing a carbon source (Step A); and purifying 2,3-butanediol from culture liquid obtained in this step (Step B). In this method, the carbon source contains pentose, and the microorganism belonging to the genus Zymobacter has a capacity to metabolize pentose.
    Type: Grant
    Filed: November 19, 2014
    Date of Patent: September 11, 2018
    Assignee: Toray Industries, Inc.
    Inventors: Kyohei Isobe, Kenji Sawai, Kenji Kawamura, Katsushige Yamada, Hideshi Yanase
  • Patent number: 10023885
    Abstract: The present invention provides an improved process for the preparation of a compound of formula (I), which comprises the steps of: formula (I), (a) reacting isovaleraldehyde of formula (II) and alkyl cyanoacetate of formula (III) optionally in presence of salts of weak acid and weak base or weak base in a suitable solvent to get 2-cyano-5-methyl-hex-2-enoic acid alkyl ester of formula (IV); (b) reacting 2-cyano-5-methyl-hex-2-enoic acid alkyl ester of formula (IV) with a suitable cyanide source in water or in an organic solvent or mixture thereof to get 2-isobutylsuccinonitrile of formula (V); (c) obtaining optionally 2-isobutylsuccinonitrile of formula (V) by reacting isovaleraldehyde of formula (II) and alkyl cyanoacetate of formula (III) in presence of suitable cyanide source in water or in an organic solvent or mixture thereof in single step; (d) converting 2-isobutylsuccinonitrile of formula (V) to racemic 3-cyano-5-methyl-hexanoic acid or salt thereof of formula (VI) with a genetically modified nitrilas
    Type: Grant
    Filed: November 4, 2013
    Date of Patent: July 17, 2018
    Assignee: Hikal Limited
    Inventors: Swapnil Surendra Mohile, Swapnil Gulabrao Yerande, Sarika Madhukarrao Lunge, Rameshkumar Maghabhai Patel, Shivaji Balbhim Gugale, Rajesh Mataprasad Thakur, Ramesh Ananda Mokal, Ashok Kumar Gangopadhyay, Peter David Nightingale
  • Patent number: 10017798
    Abstract: The present invention discloses an E. coli engineering bacteria producing 1,5pentanediamine through a whole cell catalysis and its application. The engineering bacteria according to the present invention, is Escherichia coli (E. coli) strain B or its derivative strains with the overexpression of a lysine decarboxylase gene and a proper expression of a lysinecadaverine antiporter gene cadB.
    Type: Grant
    Filed: June 25, 2015
    Date of Patent: July 10, 2018
    Assignee: NINGXIA EPPEN BIOTECH CO., LTD
    Inventors: Tingyi Wen, Shuwen Liu, Yong Liang, Xiuling Shang, Qian Liu, Jifu Wen, Yun Zhang
  • Patent number: 9976160
    Abstract: A method of producing a sugar liquid from biomass includes decomposing a fermentation inhibitor contained in a sugar aqueous solution obtained from biomass with a microorganism incapable of utilizing glucose and/or xylose or a crude enzyme derived from the microorganism, wherein the fermentation inhibitor includes one or more compounds selected from the group consisting of courmaric acid, coumaramide, ferulic acid, ferulamide, vanillin, vanillic acid, acetovanillone, furfural, and 3-hydroxymethylfurfural.
    Type: Grant
    Filed: July 8, 2014
    Date of Patent: May 22, 2018
    Assignee: Toray Industries, Inc.
    Inventors: Hiroyuki Kurihara, Hiroko Ishizuka, Katsushige Yamada
  • Patent number: 9938543
    Abstract: This document describes biochemical pathways for producing 2,4-pentadienoyl-CoA by forming one or two terminal functional groups, comprised of carboxyl or hydroxyl group, in a C5 backbone substrate such as glutaryl-CoA, glutaryl-[acp] or glutarate methyl ester. 2,4-pentadienoyl-CoA can be enzymatically converted to 1,3-butadiene.
    Type: Grant
    Filed: June 16, 2015
    Date of Patent: April 10, 2018
    Assignee: INVISTA NORTH AMERICA S.A.R.L.
    Inventors: Nadia Fatma Kadi, Mariusz Kamionka, Alexander Brett Foster, Alex Van Eck Conradie, Adriana Leonora Botes
  • Patent number: 9920336
    Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the fatty acid synthesis pathway and oxidative cleavage of long chain acyl-[acp] intermediates by a monooxgenase (e.g., cytochrome P450) such as that encoded by BioI from microorganisms such as Bacillus subtillis.
    Type: Grant
    Filed: December 23, 2013
    Date of Patent: March 20, 2018
    Assignee: INVISTA NORTH AMERICA S.A.R.L.
    Inventors: Paul S. Pearlman, Alex Van Eck Conradie, Changlin Chen, Adriana Leonora Botes
  • Patent number: 9816117
    Abstract: This document describes biochemical pathways for producing 6-hydroxyhexanoate methyl ester and hexanoic acid hexyl ester using one or more of a fatty acid O-methyltransferase, an alcohol O-acetyltransferase and a monooxygenase, as well as recombinant hosts expressing one or more of such enzymes. 6-hydroxyhexanoate methyl esters and hexanoic acid hexyl ester can be enzymatically converted to adipic acid, adipate semialdehyde, 6-aminohexanoate, 6-hydroxyhexanoate, hexamethylenediamine, and 1,6-hexanediol.
    Type: Grant
    Filed: June 16, 2015
    Date of Patent: November 14, 2017
    Assignee: INVISTA NORTH AMERICA S.A.R.L.
    Inventors: Alex Van Eck Conradie, Adriana Leonora Botes, Ramdane Haddouche
  • Patent number: 9738911
    Abstract: This document describes biochemical pathways for producing one or more of pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine and 1,7-heptanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C7 aliphatic backbone substrate produced from succinate semialdehyde or pyruvate. These pathways, metabolic engineering and cultivation strategies described herein rely on the aldol condensation of succinate semialdehyde and pyruvate.
    Type: Grant
    Filed: December 23, 2013
    Date of Patent: August 22, 2017
    Assignee: INVISTA NORTH AMERICA S.A.R.L.
    Inventors: Alex Van Eck Conradie, Adriana Leonora Botes, Changlin Chen, Paul S. Pearlman
  • Patent number: 9725746
    Abstract: The invention relates to a whole-cell catalyst which expresses a recombinant ?-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase which phosphopantetheinylates the fatty acid reductase, and which expresses, in addition to the ?-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase, a transaminase, wherein the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and also to a process for converting a carboxylic acid or dicarboxylic acid or a monoester thereof to an amine or diamine, comprising the steps of contacting the carboxylic acid or dicarboxylic acid or the monoester thereof with a phosphopantetheinylated fatty acid reductase or an ?-dioxygenase and contacting the product with a transaminase.
    Type: Grant
    Filed: December 18, 2013
    Date of Patent: August 8, 2017
    Assignee: Evonik Degussa GmbH
    Inventors: Steffen Schaffer, Jasmin Corthals, Mirja Wessel, Hans-Georg Hennemann, Harald Haeger, Michael Volland, Martin Roos
  • Patent number: 9719095
    Abstract: Disclosed herein are an expression vector capable of expressing myrcene, an Escherichia coli strain transformed with the vector and having improved capability of producing myrcene and a method for producing myrcene and a method for recycling glycerol using the same. In an aspect, the transformed Escherichia coli strain of the present disclosure can produce myrcene with high purity on a large scale using glycerol or glucose as a carbon source. Also, the Escherichia coli strain of the present disclosure is economical and environment-friendly because it can produce high value-added myrcene using waste glycerol as a carbon source. In addition, the strongly volatile myrcene can be produced and isolated at the same time.
    Type: Grant
    Filed: November 24, 2015
    Date of Patent: August 1, 2017
    Assignee: Korea Institute of Science and Technology
    Inventors: Han Min Woo, Eun Mi Kim, Youngsoon Um, Gyeongtaek Gong, Sun Mi Lee, Yunje Kim
  • Patent number: 9708588
    Abstract: The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.
    Type: Grant
    Filed: June 13, 2016
    Date of Patent: July 18, 2017
    Assignee: Codexis, Inc.
    Inventors: Jovana Nazor, Derek J. Smith, Michael A. Crowe, Shiwei Song, Steven J. Collier
  • Patent number: 9657264
    Abstract: The present invention relates to a recombinant microorganism capable of producing putrescine at high yield due to inactivated activity of a protein having an amino acid sequence represented by SEQ ID NO: 2 in the microorganism, and a method of producing putrescine using the microorganism.
    Type: Grant
    Filed: April 24, 2015
    Date of Patent: May 23, 2017
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Kyoung Min Lee, Hongxian Li, Su Jin Park, Young Lyeol Yang, Hye Won Um, Hee Kyoung Jung
  • Patent number: 9617568
    Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
    Type: Grant
    Filed: June 8, 2016
    Date of Patent: April 11, 2017
    Assignee: Codexis, Inc.
    Inventors: Christopher K. Savile, Vesna Mitchell, Xiyun Zhang, Gjalt W. Huisman
  • Patent number: 9556427
    Abstract: A method for isolating genomic DNA (gDNA) from a biological material. In some embodiments, the method includes (a) contacting a sample that contains gDNA with a solution of hydroxide and a detergent under conditions and for a time sufficient to degrade a cell wall, a cell membrane, a nuclear membrane, a nucleoprotein, or combinations thereof and/or to denature the gDNA; (b) mixing into the solution resulting from step (a) a solution characterized by high salt and sufficient buffering capacity to reduce the pH of the solution to less than 10, thereby producing a neutralized solution; (c) centrifuging the sample at a speed and length of time sufficient to clarify the preparation; and (d) removing insoluble material from the neutralized and clarified preparation, whereby a solution of gDNA is produced.
    Type: Grant
    Filed: February 4, 2014
    Date of Patent: January 31, 2017
    Assignee: Syngenta Participations AG
    Inventor: Yanshan Ji
  • Patent number: 9486434
    Abstract: Provided is a composition that contains a mixture of broccoli seed and mustard seed. Before mixing the broccoli seed with the mustard seed, the broccoli seed is subjected to baking and a pressurized heat treatment. The broccoli seed is baked at a temperature of at least 200 degrees Fahrenheit for at least 60 minutes, and is subjected to a pressurized heat treatment of at least 200 degrees Fahrenheit at a pressure of at least 10 pounds/square inch for at least 5 minutes. Also provided is a method for therapy and/or prophylaxis of bladder cancer in an individual. The method entails administering orally to the individual a composition that contains an isothiocyanate (ITC) or a derivative thereof such that the administration inhibits the growth and/or recurrence of bladder cancer. Nutraceutical compositions are also provided.
    Type: Grant
    Filed: September 11, 2012
    Date of Patent: November 8, 2016
    Assignee: Health Research Inc.
    Inventors: Yuesheng Zhang, Arup Bhattacharya, Li Tang, Yun Li, Joseph D. Paonessa, Feng Geng, Yi Ding
  • Patent number: 9476071
    Abstract: Process for the enzymatic synthesis of the compound of formula (I): comprising enantioselective enzymatic hydrolysis of the nitrile of formula (IV): using the nitrilase of Rhodococcus rhodochrous of EMBL accession number EF467367.1, and the application of such a process in the synthesis of ivabradine and addition salts thereof with a pharmaceutically acceptable acid.
    Type: Grant
    Filed: February 26, 2014
    Date of Patent: October 25, 2016
    Assignee: LES LABORATOIRES SERVIER
    Inventors: Sandrine Pedragosa-Moreau, François Lefoulon
  • Patent number: 9415346
    Abstract: The present invention discloses a process for the recovery of betaines from electrodialysis (ED) waste streams, whereby said waste streams are treated with a pressure driven membrane process.
    Type: Grant
    Filed: February 3, 2010
    Date of Patent: August 16, 2016
    Assignee: Lonza Ltd.
    Inventors: Markus Bicker, Miguel Angel Caraucán Dávila
  • Patent number: 9404118
    Abstract: The invention relates to genetically modified Pichia ciferrii cells, to the use thereof and to a method of producing sphingoid bases and sphingolipids.
    Type: Grant
    Filed: July 23, 2012
    Date of Patent: August 2, 2016
    Assignee: EVONIK DEGUSSA GMBH
    Inventors: Tim Köhler, Christoph Schorsch, Eckhard Boles, Heiko Andrea, Mike Farwick, Steffen Schaffer
  • Patent number: 9388438
    Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
    Type: Grant
    Filed: July 15, 2015
    Date of Patent: July 12, 2016
    Assignee: Codexis, Inc.
    Inventors: Christopher K. Savile, Vesna Mitchell, Xiyun Zhang, Gjalt W. Huisman
  • Patent number: 9303277
    Abstract: The present invention relates to a microorganism having an improved ornithine-producing ability, in which the biosynthetic pathway of arginine form ornithine is blocked, the intracellular glutamate level is increased, and the biosynthetic pathway of ornithine from glutamate is enhanced, and a method for producing ornithine using the microorganism.
    Type: Grant
    Filed: December 8, 2011
    Date of Patent: April 5, 2016
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Hyang Choi, Kyoung Min Lee, Min Sun Kang, Sung Hoo Jhon, Hye Won Um, Su Jin Choi, Han Won Lee, Soo An Shin
  • Patent number: 9150890
    Abstract: The invention relates to genetically engineered Candida tropicalis cells, use thereof and a method of production of ?-hydroxycarboxylic acids and ?-hydroxycarboxylic acid esters.
    Type: Grant
    Filed: January 30, 2015
    Date of Patent: October 6, 2015
    Assignee: EVONIK DEGUSSA GmbH
    Inventors: Markus Poetter, Hans-Georg Hennemann, Steffen Schaffer, Thomas Haas
  • Patent number: 9121045
    Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
    Type: Grant
    Filed: October 2, 2014
    Date of Patent: September 1, 2015
    Assignee: Codexis, Inc.
    Inventors: Christopher K. Savile, Vesna Mitchell, Xiyun Zhang, Gjalt W. Huisman
  • Patent number: 9115362
    Abstract: The present invention relates to a mutant microorganism having a high ability to produce cadaverine, and a method for producing cadaverine using the same. More specifically, the invention relates to a mutant microorganism having a high ability to produce cadaverine wherein a gene involved in the cadaverine degradation or utilization pathway is inactivated or deleted, and to a method for producing cadaverine in high yield by culturing the mutant microorganism under aerobic conditions. The mutant microorganism according to the present invention is useful for producing a high yield of cadaverine which can be widely used in various industrial applications.
    Type: Grant
    Filed: August 3, 2011
    Date of Patent: August 25, 2015
    Assignee: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Sang Yup Lee, Zhi Gang Qian, Xiaoxia Xia, Min Young Kim
  • Patent number: 9068175
    Abstract: Disclosed are preparations of modified interfacial enzymes, particularly lipases and phospholipases, immobilized on a solid support, wherein the enzyme is surrounded by hydrophobic microenvironment, thereby protected from deactivation and/or aggregation in the presence of hydrophilic agents, substrates and/or reaction products. The enzyme may be protected by being covalently bonded with lipid groups which coat the enzyme, or by being immobilized or embedded in a hydrophobic solid support. Also disclosed are processes for the preparation of the hydrophobically protected enzymes. The enzymes may be efficiently used in the preparation of biodiesel.
    Type: Grant
    Filed: May 7, 2008
    Date of Patent: June 30, 2015
    Assignee: Trans Bio-Diesel Ltd.
    Inventor: Sobhi Basheer
  • Publication number: 20150140617
    Abstract: The invention relates to genetically engineered Candida tropicalis cells, use thereof and a method of production of ?-hydroxycarboxylic acids and ?-hydroxycarboxylic acid esters.
    Type: Application
    Filed: January 30, 2015
    Publication date: May 21, 2015
    Applicant: EVONIK DEGUSSA GMBH
    Inventors: Markus POETTER, Hans-Georg Hennemann, Steffen Schaffer, Thomas Haas
  • Publication number: 20150132807
    Abstract: The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
    Type: Application
    Filed: November 12, 2014
    Publication date: May 14, 2015
    Inventors: Nicholas J. Agard, Oscar Alvizo, Melissa Ann Mayo, Stefanie Ng Minor, James Nicholas Riggins, Jeffrey C. Moore
  • Publication number: 20150132808
    Abstract: A mutant lysine decarboxylase is produced by replacing at least one of the amino-acids in the amino acid sequence shown in sequence ID No. 4 of the sequence listing with another amino-acid.
    Type: Application
    Filed: January 17, 2013
    Publication date: May 14, 2015
    Inventors: Daisuke Mochizuki, Tadashi Araki, Akiko Natsuji, Tomomi Sakai, Tomonori Hidesaki
  • Patent number: 9029106
    Abstract: The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.
    Type: Grant
    Filed: June 18, 2013
    Date of Patent: May 12, 2015
    Assignee: Codexis, Inc.
    Inventors: Ish K. Dhawan, Gregory Miller, Xiyun Zhang
  • Patent number: 9023624
    Abstract: The present invention is related to a method for the fermentative production of hydroxymethionine, comprising the steps of: culturing a recombinant microorganism modified to produce methionine in an appropriate culture medium comprising a source of carbon, a source of sulfur and a source of nitrogen, recovering hydroxymethionine from the culture medium. In a specific embodiment, the recombinant microorganism is cultivated under conditions of nitrogen limitation. The invention is also related to the biologically-produced hydroxymethionine and its uses.
    Type: Grant
    Filed: December 30, 2010
    Date of Patent: May 5, 2015
    Assignee: Metabolic Explorer
    Inventors: Rainer Figge, Wanda Dischert
  • Publication number: 20150118721
    Abstract: The invention relates to novel enzymes that provide acetylated sphingoid bases.
    Type: Application
    Filed: March 13, 2013
    Publication date: April 30, 2015
    Applicant: EVONIK INDUSTRIES AG
    Inventors: Steffen Schaffer, Mike Farwick, Heiko Andrea, Tim Koehler, Daniel Wolff, Frank ter Veld, Ansgar Poetsch, Eckhard Boles, Christoph Schorsch
  • Publication number: 20150111262
    Abstract: This document describes biochemical pathways for producing glutaric acid, 5-aminopentanoic acid, 5-hydroxypentanoic acid, cadaverine or 1,5-pentanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C5 backbone substrate such as D-proline.
    Type: Application
    Filed: August 27, 2014
    Publication date: April 23, 2015
    Inventors: Adriana Leonora Botes, Alex Van Eck Conradie
  • Publication number: 20150111253
    Abstract: The invention relates to a method for oxidizing a fatty acid or an ester thereof of formula (I) H3C—(CH2)n-COOR, wherein R is selected from the group that comprises H, methyl, ethyl, propyl, and butyl, wherein n is 0 to 30, preferably 6 to 24, comprising the step of oxidizing the fatty acid or the ester thereof by contacting the fatty acid or the ester thereof with a cytochrome P450 monooxygenase of the CYP153 family in the presence of molecular oxygen and NAD(P)H and a whole-cell catalyst that expresses a recombinant cytochrome P450 monooxygenase of the CYP153 family, a recombinant alcohol dehydrogenase, a recombinant transaminase, and optionally one or more than one recombinant enzyme from the group comprising alanine dehydrogenase, ferredoxin, and ferredoxin reductase, and the use of said whole-cell catalyst to oxidize a fatty acid or an ester thereof.
    Type: Application
    Filed: March 12, 2013
    Publication date: April 23, 2015
    Applicant: EVONIK INDUSTRIES AG
    Inventors: Steffen Schaffer, Michaela Hauberg, Mirja Wessel, Hans-Georg Hennemann, Jan Christoph Pfeffer, Thomas Haas, Harald Haeger
  • Publication number: 20150104838
    Abstract: The present invention relates to a recombinant microorganism having enhanced ability of producing putrescine at high yield, which is generated by weakening the activity of NCgl1469 in a microorganism of Corynebacterium genus that is modified to produce putrescine, and a method for producing putrescine using the same.
    Type: Application
    Filed: January 11, 2013
    Publication date: April 16, 2015
    Inventors: Su Jin Choi, Hyang Choi, Min Sun Kang, Sung Hoo Jhon, Kyoung Min Lee, Hye Won Um, Young Lyeol Yang
  • Patent number: 8999681
    Abstract: A method of producing cadaverine is more efficient and at a higher yield than production methods by the conventional fermentation methods. The method includes culturing coryneform bacterium/bacteria having a resistance to a pH of 5.5 or less. Preferably, the coryneform bacterium/bacteria has/have lysine decarboxylase activity and, preferably, the coryneform bacterium/bacteria has/have homoserine auxotrophy and/or a resistance to S-(2-aminoethyl)-L-cysteine.
    Type: Grant
    Filed: December 8, 2011
    Date of Patent: April 7, 2015
    Assignee: Toray Industries, Inc.
    Inventors: Nanami Sasaki, Takashi Mimitsuka, Hideki Sawai, Kenji Sawai
  • Patent number: 8999452
    Abstract: The present invention provides a surface-independent surface-modifying multifunctional biocoating and methods of application thereof. The method comprises contacting at least a portion of a substrate with an alkaline solution comprising a surface-modifying agent (SMA) such as dopamine so as to modify the substrate surface to include at least one reactive moiety. In another version of the invention, a secondary reactive moiety is applied to the SMA-treated substrate to yield a surface-modified substrate having a specific functionality.
    Type: Grant
    Filed: July 11, 2013
    Date of Patent: April 7, 2015
    Assignee: Northwestern University
    Inventors: Phillip B. Messersmith, Haeshin Lee
  • Patent number: 8999680
    Abstract: The present invention relates to a novel method for the preparation of 1,4-diaminobutane [DAB]. The method according to the present invention involves at least one biocatalytic step which comprises the biocatalytic production of at least one N-protected precursor of DAB. The present invention also relates to a method for the preparation of DAB involving at least one biocatalytic step, and comprising the steps of a) biocatalytically preparing an N-protected precursor of DAB yielding a—biocatalytic reaction mixture containing the N-protected precursor of DAB, b) recovering the N-protected precursor from the biocatalytic reaction mixture and c) converting the N-protected precursor into DAB. More in particular, the present invention relates to a method for the preparation of DAB, wherein the at least N-protected precursor of DAB is selected from the group consisting of N5-protected ornithine, N-protected DAB, and N-protected 4-aminobutyraldehyde.
    Type: Grant
    Filed: July 20, 2010
    Date of Patent: April 7, 2015
    Assignee: DSM IP Assets B.V.
    Inventors: Liang Wu, Petronella Catharina Raemakers-Franken
  • Publication number: 20150087031
    Abstract: The present invention relates to methods of processing lignocellulosic material to obtain hemicellulose sugars, cellulose sugars, lignin, cellulose and other high-value products. Also provided are hemicellulose sugars, cellulose sugars, lignin, cellulose, and other high-value products.
    Type: Application
    Filed: May 3, 2013
    Publication date: March 26, 2015
    Inventors: Robert Jansen, Claire Gregoire, philip Travisano, Lee Madsen, Neta Matis, Yael Har-Tal, Shay Eliahu, James Alan Lawson, Noa Lapidot, Luke Burke, Aharon M. Eyal, Timothy Allen Bauer, Hagit Sade, Paul Mcwilliams, Ziv-Vladimir Belman, Bassem Hallac, Michael Zviely, Yelena Gershinksy, Adam Carden
  • Publication number: 20150087028
    Abstract: The invention relates to recombinant expression of variant forms of M. thermophila CBH1a and homologs thereof, having improved thermoactivity, specific activity, and other desirable properties. Also provided are methods for producing ethanol and other valuable organic compounds by combining cellobiohydrolase variants with cellulosic materials.
    Type: Application
    Filed: March 12, 2013
    Publication date: March 26, 2015
    Inventors: Behnaz Behrouzian, Xinkai Xie, Kui Chan, Xiyun Zhang, Vesna Mitchell, Douglas A. Hattendorf
  • Patent number: 8986948
    Abstract: The present invention relates to the use of nucleic acid molecules coding for a bacterial xylose isomerase (XI), preferably coming from Clostridium phytofermentans, for reaction/metabolization, particularly fermentation, of recombinant microorganisms of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol, by means of xylose fermenting yeasts. The present invention further relates to cells, particularly eukaryotic cells, which are transformed utilizing a nucleic acid expression construct which codes for a xylose isomerase, wherein the expression of the nucleic acid expression construct imparts to the cells the capability to directly isomerize xylose into xylulose. Said cells are preferably utilized for reaction/metabolization, particularly fermentation, of biomaterial containing xylose, and particularly for the production of bioalcohols, particularly bioethanol.
    Type: Grant
    Filed: July 1, 2009
    Date of Patent: March 24, 2015
    Assignee: Lesaffre et Compagnie
    Inventors: Dawid Brat, Eckhard Boles, Marco Keller, Beate Wiedemann
  • Publication number: 20150079640
    Abstract: The present disclosure relates to methods of using transaminase polypeptides in the synthesis of chiral amines from prochiral ketones.
    Type: Application
    Filed: November 19, 2014
    Publication date: March 19, 2015
    Inventors: Gregory Hughes, Paul N. Devine, Fred J. Fleitz, Brendan T. Grau, John Limanto, Christopher Savile, Emily Mundorff
  • Patent number: 8980578
    Abstract: The present invention provides fungal xylanase and/or beta-xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase and/or beta-xylosidases, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.
    Type: Grant
    Filed: June 10, 2013
    Date of Patent: March 17, 2015
    Assignee: Codexis, Inc.
    Inventors: Ryan Fong, Xiyun Zhang, Chris Noriega, Nicholas Agard, Anupam Gohel, Derek Smith
  • Publication number: 20150057465
    Abstract: The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.
    Type: Application
    Filed: March 17, 2014
    Publication date: February 26, 2015
    Inventors: Hans LIAO, Christopher Patrick MERCOGLIANO, Travis Robert WOLTER, Michael Tai Man LOUIE, Wendy Kathleen RIBBLE, Tanya LIPSCOMB, Eileen Colie SPINDLER, Michael D. LYNCH
  • Publication number: 20150057461
    Abstract: The present invention relates to a transformant which is transformed to express Baeyer-Villiger monooxygenase (BVMO), a method for producing C5-C14 medium-chain ?-hydroxy fatty acids, ?,?-dicarboxylic acids, ?-amino fatty acids, or alcohols from C16-C20 long-chain fatty acids by biotransformation using the transformant, a method for producing a fatty acid derivative having an ester group which is introduced into the chain thereof from keto fatty acid using the BVMO, and novel ?-hydroxy fatty acids which are prepared by the method. Degradation products such as C5 to C14 ?-hydroxy fatty acids, ?,?-dicarboxylic acids, ?-amino fatty acids, alcohols can be produced in a large amount from C16 to C20 long-chain fatty acids contained in a medium by biotransformation using a transformant capable of expressing BVMO of the present invention. Therefore, it can be widely used to produce ?-hydroxy fatty acids, ?,?-dicarboxylic acids, ?-amino fatty acids or alcohols in a more safe and economic manner.
    Type: Application
    Filed: April 5, 2013
    Publication date: February 26, 2015
    Inventors: Jin Byung Park, Ji Won Song, Eun Yeong Jeon
  • Publication number: 20150056669
    Abstract: The present invention provides various combinations of genetic modifications to a transformed host cell that provide increase conversion of carbon to a chemical product. The present invention also provides methods of fermentation and methods of making various chemical products.
    Type: Application
    Filed: March 17, 2014
    Publication date: February 26, 2015
    Inventors: Hans LIAO, Christopher Patrick MERCOGLIANO, Travis Robert WOLTER, Michael Tai Man LOUIE, Wendy Kathleen RIBBLE, Tanya LIPSCOMB, Eileen Colie SPINDLER, Michael D LYNCH
  • Publication number: 20150056665
    Abstract: A method of producing a chemical includes culturing cells in a culture solution in a fermentor to ferment a feedstock to produce a chemical; supplying the culture solution containing the chemical produced in the culturing to a plurality of separation membrane units arranged in parallel; filtering the culture solution supplied in the supplying to separate a permeate containing the chemical; refluxing a retentate that is not filtered in the filtering to the fermentor; and supplying a gas containing oxygen to the plurality of separation membrane units while a supply amount is changed to at least two different values to perform scrubbing, wherein the supply amount and supply time of the gas containing oxygen supplied in the culturing and the supplying the gas are set so that a kLa value is within a predetermined range from an optimal kLa value for the cells cultured in the culturing.
    Type: Application
    Filed: March 27, 2013
    Publication date: February 26, 2015
    Inventors: Satoko Kanamori, Hideki Sawai, Norihiro Takeuchi
  • Publication number: 20150050706
    Abstract: Disclosed is a process for carrying out a cyclisation reaction, a polymerization reaction, an enzymatic reaction showing substrate inhibition, an enzymatic reaction showing product inhibition, a reaction showing precipitation of the substrate or of a reactant, the process comprising the steps of a) diluting a fresh substrate with solvent to form a diluted substrate-solvent mixture, and supplying this mixture to a reactor, b) causing the reaction medium in the reactor to react, c) discharging reaction mixture comprising reaction product, solvent, and substrate that has not reacted, to a first filtration membrane which is permeable to the solvent and impermeable to the substrate and to the catalyst or at least one of the reactants, d) returning solvent from the permeate side of the first membrane to dilute the fresh substrate, and e) returning retentate comprising substrate which has not reacted, from the first filtration membrane to the reactor.
    Type: Application
    Filed: April 19, 2013
    Publication date: February 19, 2015
    Inventors: Anita Buekenhoudt, Pieter Vandezande, Dominique Ormerod