Preparing Nitrogen-containing Organic Compound Patents (Class 435/128)
  • Patent number: 11001865
    Abstract: Disclosed are methods of making a modified lecithin by conducting an enzymatic conversion of a naturally derived lecithin to form a modified lecithin, e.g., having an enhanced level of phosphatidylethanolamine, phosphatidylserine, or a combination thereof. Compositions prepared from the modified lecithin and use to inhibit lipid oxidation are described.
    Type: Grant
    Filed: June 14, 2018
    Date of Patent: May 11, 2021
    Assignee: THE UNIVERSITY OF MASSACHUSETTS
    Inventor: Eric Decker
  • Patent number: 10988743
    Abstract: Provided is a microorganism that is capable of efficiently producing para-aminobenzoic acid (4-ABA) or a salt thereof, using saccharides as raw materials, and a method for efficiently producing 4-ABA or a salt thereof by using this microorganism. A transformant obtained by introducing, into a coryneform bacterium, a gene that encodes 4-amino-4-deoxychorismate lyase, a gene that encodes a para-aminobenzoate synthase component I, and a gene that encodes a para-aminobenzoate synthase component II, is capable of efficiently producing 4-ABA or a salt thereof from saccharides.
    Type: Grant
    Filed: February 24, 2017
    Date of Patent: April 27, 2021
    Assignees: RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTH, SUMITOMO BAKELITE CO., LTD.
    Inventors: Masayuki Inui, Kazumi Hiraga, Masako Suda, Takeshi Kubota
  • Patent number: 10801047
    Abstract: The present disclosure relates to a putrescine-producing microorganism of the genus Corynebacterium, and a method of producing putrescine using the same.
    Type: Grant
    Filed: July 19, 2018
    Date of Patent: October 13, 2020
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Kyoung Min Lee, Sung Gun Lee, Seon Hye Kim, Kyungsu Na, Hong Xian Li, Hyun-jung Bae, Young Lyeol Yang, Hye Won Um, Hyo Hyoung Lee
  • Patent number: 10801046
    Abstract: This document describes biochemical pathways for producing a difunctional product having an odd number of carbon atoms in vitro or in a recombinant host, or salts or derivatives thereof, by forming two terminal functional groups selected from carboxyl, amine, formyl, and hydroxyl groups in an aliphatic carbon chain backbone having an odd number of carbon atoms synthesized from (i) acetyl-CoA and propanedioyl-CoA via one or more cycles of methyl ester shielded carbon chain elongation or (ii) propanedioyl-[acp] via one or more cycles of methyl ester shielded carbon chain elongation. The biochemical pathways and metabolic engineering and cultivation strategies described herein rely on enzymes or homologs accepting methyl ester shielded aliphatic carbon chain backbones and maintaining the methyl ester shield for at least one further enzymatic step following one or more cycles of methyl ester shielded carbon chain elongation.
    Type: Grant
    Filed: July 25, 2017
    Date of Patent: October 13, 2020
    Assignee: INVISTA North America S.a.r.l.
    Inventors: Alexander Brett Foster, Stephen Thomas Cartman, Jonathan Kennedy, William Joseph Simmons
  • Patent number: 10781468
    Abstract: The invention relates to a medium for producing glucosamine, including 25-500 g/L molasses, 0.01-100 g/L soybean hydrolysate or 0.25-100 g/L corn steep liquor, 0.1-2 g/L MgSO4.7H2O, 0.1-0.5 g/L Al(NO3)3, and 1-5 mL/L methanol. The invention also relates to a method for producing glucosamine, including providing microorganism being able to produce glucosamine, and fermenting the microorganism in the medium mentioned above.
    Type: Grant
    Filed: October 26, 2017
    Date of Patent: September 22, 2020
    Assignee: YUAN ZE UNIVERSITY
    Inventors: Ho-Shing Wu, Jian-Hao Chen
  • Patent number: 10774356
    Abstract: The invention relates to transaminases and their use. The ATAs according to the invention are particularly useful for catalyzing the conversion of amines to ketones and/or vice versa. Preferably, the transaminase (ATA) according to the invention comprises an amino acid sequence with at least 80% homology to SEQ ID NO:1, wherein the amino acid sequence is engineered compared to SEQ ID NO:1 such that it comprises at least a substitution selected from the group consisting of F255L, F255A, F255C, F255D, F255E, F255G, F255H, F255K, F255M, F255N, F255P, F255Q, F255R, F255S, F255T, F255V, F255W, and F255Y.
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: September 15, 2020
    Assignee: C-LEcta GmbH
    Inventors: Andreas Vogel, Daniel Schwarze, Rico Czaja, Sally Bayer, Sebastian Bartsch
  • Patent number: 10724012
    Abstract: An isolated glycosyltransferase (GT) polypeptide capable of: (I): conjugating glucose to flavokermesic acid (FK); and/or (II): conjugating glucose to kermesic acid (KA) and use of this GT to e.g. make Carminic acid.
    Type: Grant
    Filed: October 15, 2018
    Date of Patent: July 28, 2020
    Assignees: Københavns Universitet, Danmarks Tekniske Universitet
    Inventors: Rubini Maya Kannangara, Mads Bennedsen, Bjørn Madsen, Kim Binderup, Ulf Thrane, Rasmus John Normand Frandsen, Uffe Hasbro Mortensen, Birger Lindberg Møller, Finn Thyge Okkels
  • Patent number: 10711289
    Abstract: Provided is a method of producing and isolating a diamine produced by microbial fermentation that minimizes undesirable salt formation to provide a lower cost process.
    Type: Grant
    Filed: December 22, 2015
    Date of Patent: July 14, 2020
    Assignee: GENOMATICA, INC.
    Inventors: Lauri H. Suominen, Connor J. Galleher, Michael Japs, Mark J. Burk, Cara Tracewell
  • Patent number: 10683258
    Abstract: The present invention relates to a composition which comprises at least one ?,?-amino-alkanoic acid or ester and at least one alkanoic ester or acid, characterised in that: the ?,?-amino-alkanoic acid or ester has formula H2N—(CH2)n-COOR, wherein n=9 to 13 and R is an alkyl group or a hydrogen; the alkanoic ester or acid has formula R1—COOR2, wherein R1 is a straight or branched alkyl group with formula CmH2m+1 or CmH2m?3, wherein m=6 to 20 and R2 is an alkyl group or a hydrogen; the molar ratio between the alkanoic acid(s) or ester(s) and the ?,?-amino-alkanoic acid(s) or ester(s) is in the range from 0.001 to 0.4%. The present invention also relates to novel methods for obtaining such a composition.
    Type: Grant
    Filed: September 28, 2016
    Date of Patent: June 16, 2020
    Assignee: ARKEMA FRANCE
    Inventors: Thierry Briffaud, Jean-Luc Couturier, Jean-Luc Dubois, Jean-François Devaux
  • Patent number: 10640797
    Abstract: The present invention relates to a microorganism for producing diamine, in which activity of a protein having an amino acid sequence of SEQ ID NO: 6 or an amine acid sequence having 42% or higher sequence homology with SEQ ID NO: 6 is introduced or enhanced, and a method of producing diamine using the same.
    Type: Grant
    Filed: March 27, 2015
    Date of Patent: May 5, 2020
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Kyoung Min Lee, Su Jin Park, Hee Kyoung Jung, Young Lyeol Yang, Hong Xian Li, Hye Won Um
  • Patent number: 10544402
    Abstract: The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.
    Type: Grant
    Filed: June 15, 2017
    Date of Patent: January 28, 2020
    Assignee: Codexis Inc.
    Inventors: Jovana Nazor, Derek J. Smith, Michael A. Crowe, Shiwei Song, Steven J. Collier
  • Patent number: 10227621
    Abstract: The present invention relates to methods for preparing an aqueous acrylamide solution having a low acrylic acid concentration. In addition, the present invention relates to methods for reducing the acrylic acid concentration of an aqueous acrylamide solution. The methods involve a bioconversion of acrylonitrile to acrylamide in the presence of a biocatalyst, wherein during the bioconversion the content of acrylonitrile is maintained at 0.3 w/w % or more referred to the total weight of the composition in the reactor. Also provided is an aqueous acrylamide solution which is obtained by the methods of the present invention. Furthermore, the present invention is related to an acrylamide homopolymer or copolymer obtained by polymerizing the acrylamide of the aqueous solution.
    Type: Grant
    Filed: September 30, 2015
    Date of Patent: March 12, 2019
    Assignee: BASF SE
    Inventors: Michael Guenter Braun, Juergen Daeuwel, Hans-Juergen Lang, Peter Oedman, Kai-Uwe Baldenius, Matthias Kleiner, Michael Kiefer, Stephan Freyer, Michael Budde
  • Patent number: 10221433
    Abstract: The present invention relates to a recombinant microorganism capable of producing putrescine, in which the microorganism is modified to have enhanced NCgl2522 activity, thereby producing putrescine in a high yield, and a method for producing putrescine using the microorganism.
    Type: Grant
    Filed: February 25, 2014
    Date of Patent: March 5, 2019
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Kyoung Min Lee, Hee Kyoung Jung, Young Lyeol Yang, Hye Won Um, Chang Gyeom Kim, Hong Xian Li, Su Jin Park, Jong Hyun Yoon, Baek Seok Lee, Sun Young Lee
  • Patent number: 10196667
    Abstract: The present disclosure relates to the use of an amino acid dehydrogenase in combination with a cofactor regenerating system comprising a ketoreductase. In particular embodiments, the process can be used to prepare L-tert-leucine using a leucine dehydrogenase.
    Type: Grant
    Filed: June 20, 2017
    Date of Patent: February 5, 2019
    Assignee: Codexis, Inc.
    Inventors: Fabien Louis Cabirol, Steven J. Collier, Thomas Daussmann, Naga K. Modukuru
  • Patent number: 10160985
    Abstract: The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
    Type: Grant
    Filed: March 8, 2018
    Date of Patent: December 25, 2018
    Assignee: Codexis, Inc.
    Inventors: Christopher K. Savile, Emily Mundorff, Jeffrey C. Moore, Paul N. Devine, Jacob M. Janey
  • Patent number: 10072275
    Abstract: A method of producing 2,3-butanediol includes the steps of: culturing a microorganism belonging to the genus Zymobacter in a fermentation feedstock containing a carbon source (Step A); and purifying 2,3-butanediol from culture liquid obtained in this step (Step B). In this method, the carbon source contains pentose, and the microorganism belonging to the genus Zymobacter has a capacity to metabolize pentose.
    Type: Grant
    Filed: November 19, 2014
    Date of Patent: September 11, 2018
    Assignee: Toray Industries, Inc.
    Inventors: Kyohei Isobe, Kenji Sawai, Kenji Kawamura, Katsushige Yamada, Hideshi Yanase
  • Patent number: 10023885
    Abstract: The present invention provides an improved process for the preparation of a compound of formula (I), which comprises the steps of: formula (I), (a) reacting isovaleraldehyde of formula (II) and alkyl cyanoacetate of formula (III) optionally in presence of salts of weak acid and weak base or weak base in a suitable solvent to get 2-cyano-5-methyl-hex-2-enoic acid alkyl ester of formula (IV); (b) reacting 2-cyano-5-methyl-hex-2-enoic acid alkyl ester of formula (IV) with a suitable cyanide source in water or in an organic solvent or mixture thereof to get 2-isobutylsuccinonitrile of formula (V); (c) obtaining optionally 2-isobutylsuccinonitrile of formula (V) by reacting isovaleraldehyde of formula (II) and alkyl cyanoacetate of formula (III) in presence of suitable cyanide source in water or in an organic solvent or mixture thereof in single step; (d) converting 2-isobutylsuccinonitrile of formula (V) to racemic 3-cyano-5-methyl-hexanoic acid or salt thereof of formula (VI) with a genetically modified nitrilas
    Type: Grant
    Filed: November 4, 2013
    Date of Patent: July 17, 2018
    Assignee: Hikal Limited
    Inventors: Swapnil Surendra Mohile, Swapnil Gulabrao Yerande, Sarika Madhukarrao Lunge, Rameshkumar Maghabhai Patel, Shivaji Balbhim Gugale, Rajesh Mataprasad Thakur, Ramesh Ananda Mokal, Ashok Kumar Gangopadhyay, Peter David Nightingale
  • Patent number: 10017798
    Abstract: The present invention discloses an E. coli engineering bacteria producing 1,5pentanediamine through a whole cell catalysis and its application. The engineering bacteria according to the present invention, is Escherichia coli (E. coli) strain B or its derivative strains with the overexpression of a lysine decarboxylase gene and a proper expression of a lysinecadaverine antiporter gene cadB.
    Type: Grant
    Filed: June 25, 2015
    Date of Patent: July 10, 2018
    Assignee: NINGXIA EPPEN BIOTECH CO., LTD
    Inventors: Tingyi Wen, Shuwen Liu, Yong Liang, Xiuling Shang, Qian Liu, Jifu Wen, Yun Zhang
  • Patent number: 9976160
    Abstract: A method of producing a sugar liquid from biomass includes decomposing a fermentation inhibitor contained in a sugar aqueous solution obtained from biomass with a microorganism incapable of utilizing glucose and/or xylose or a crude enzyme derived from the microorganism, wherein the fermentation inhibitor includes one or more compounds selected from the group consisting of courmaric acid, coumaramide, ferulic acid, ferulamide, vanillin, vanillic acid, acetovanillone, furfural, and 3-hydroxymethylfurfural.
    Type: Grant
    Filed: July 8, 2014
    Date of Patent: May 22, 2018
    Assignee: Toray Industries, Inc.
    Inventors: Hiroyuki Kurihara, Hiroko Ishizuka, Katsushige Yamada
  • Patent number: 9938543
    Abstract: This document describes biochemical pathways for producing 2,4-pentadienoyl-CoA by forming one or two terminal functional groups, comprised of carboxyl or hydroxyl group, in a C5 backbone substrate such as glutaryl-CoA, glutaryl-[acp] or glutarate methyl ester. 2,4-pentadienoyl-CoA can be enzymatically converted to 1,3-butadiene.
    Type: Grant
    Filed: June 16, 2015
    Date of Patent: April 10, 2018
    Assignee: INVISTA NORTH AMERICA S.A.R.L.
    Inventors: Nadia Fatma Kadi, Mariusz Kamionka, Alexander Brett Foster, Alex Van Eck Conradie, Adriana Leonora Botes
  • Patent number: 9920336
    Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the fatty acid synthesis pathway and oxidative cleavage of long chain acyl-[acp] intermediates by a monooxgenase (e.g., cytochrome P450) such as that encoded by BioI from microorganisms such as Bacillus subtillis.
    Type: Grant
    Filed: December 23, 2013
    Date of Patent: March 20, 2018
    Assignee: INVISTA NORTH AMERICA S.A.R.L.
    Inventors: Paul S. Pearlman, Alex Van Eck Conradie, Changlin Chen, Adriana Leonora Botes
  • Patent number: 9816117
    Abstract: This document describes biochemical pathways for producing 6-hydroxyhexanoate methyl ester and hexanoic acid hexyl ester using one or more of a fatty acid O-methyltransferase, an alcohol O-acetyltransferase and a monooxygenase, as well as recombinant hosts expressing one or more of such enzymes. 6-hydroxyhexanoate methyl esters and hexanoic acid hexyl ester can be enzymatically converted to adipic acid, adipate semialdehyde, 6-aminohexanoate, 6-hydroxyhexanoate, hexamethylenediamine, and 1,6-hexanediol.
    Type: Grant
    Filed: June 16, 2015
    Date of Patent: November 14, 2017
    Assignee: INVISTA NORTH AMERICA S.A.R.L.
    Inventors: Alex Van Eck Conradie, Adriana Leonora Botes, Ramdane Haddouche
  • Patent number: 9738911
    Abstract: This document describes biochemical pathways for producing one or more of pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine and 1,7-heptanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C7 aliphatic backbone substrate produced from succinate semialdehyde or pyruvate. These pathways, metabolic engineering and cultivation strategies described herein rely on the aldol condensation of succinate semialdehyde and pyruvate.
    Type: Grant
    Filed: December 23, 2013
    Date of Patent: August 22, 2017
    Assignee: INVISTA NORTH AMERICA S.A.R.L.
    Inventors: Alex Van Eck Conradie, Adriana Leonora Botes, Changlin Chen, Paul S. Pearlman
  • Patent number: 9725746
    Abstract: The invention relates to a whole-cell catalyst which expresses a recombinant ?-dioxygenase or the combination of a recombinant fatty acid reductase and a phosphopantetheinyl transferase which phosphopantetheinylates the fatty acid reductase, and which expresses, in addition to the ?-dioxygenase and/or the combination of fatty acid reductase and phosphopantetheinyl transferase, a transaminase, wherein the phosphopantetheinyl transferase and/or transaminase is preferably recombinant; and also to a process for converting a carboxylic acid or dicarboxylic acid or a monoester thereof to an amine or diamine, comprising the steps of contacting the carboxylic acid or dicarboxylic acid or the monoester thereof with a phosphopantetheinylated fatty acid reductase or an ?-dioxygenase and contacting the product with a transaminase.
    Type: Grant
    Filed: December 18, 2013
    Date of Patent: August 8, 2017
    Assignee: Evonik Degussa GmbH
    Inventors: Steffen Schaffer, Jasmin Corthals, Mirja Wessel, Hans-Georg Hennemann, Harald Haeger, Michael Volland, Martin Roos
  • Patent number: 9719095
    Abstract: Disclosed herein are an expression vector capable of expressing myrcene, an Escherichia coli strain transformed with the vector and having improved capability of producing myrcene and a method for producing myrcene and a method for recycling glycerol using the same. In an aspect, the transformed Escherichia coli strain of the present disclosure can produce myrcene with high purity on a large scale using glycerol or glucose as a carbon source. Also, the Escherichia coli strain of the present disclosure is economical and environment-friendly because it can produce high value-added myrcene using waste glycerol as a carbon source. In addition, the strongly volatile myrcene can be produced and isolated at the same time.
    Type: Grant
    Filed: November 24, 2015
    Date of Patent: August 1, 2017
    Assignee: Korea Institute of Science and Technology
    Inventors: Han Min Woo, Eun Mi Kim, Youngsoon Um, Gyeongtaek Gong, Sun Mi Lee, Yunje Kim
  • Patent number: 9708588
    Abstract: The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.
    Type: Grant
    Filed: June 13, 2016
    Date of Patent: July 18, 2017
    Assignee: Codexis, Inc.
    Inventors: Jovana Nazor, Derek J. Smith, Michael A. Crowe, Shiwei Song, Steven J. Collier
  • Patent number: 9657264
    Abstract: The present invention relates to a recombinant microorganism capable of producing putrescine at high yield due to inactivated activity of a protein having an amino acid sequence represented by SEQ ID NO: 2 in the microorganism, and a method of producing putrescine using the microorganism.
    Type: Grant
    Filed: April 24, 2015
    Date of Patent: May 23, 2017
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Kyoung Min Lee, Hongxian Li, Su Jin Park, Young Lyeol Yang, Hye Won Um, Hee Kyoung Jung
  • Patent number: 9617568
    Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
    Type: Grant
    Filed: June 8, 2016
    Date of Patent: April 11, 2017
    Assignee: Codexis, Inc.
    Inventors: Christopher K. Savile, Vesna Mitchell, Xiyun Zhang, Gjalt W. Huisman
  • Patent number: 9556427
    Abstract: A method for isolating genomic DNA (gDNA) from a biological material. In some embodiments, the method includes (a) contacting a sample that contains gDNA with a solution of hydroxide and a detergent under conditions and for a time sufficient to degrade a cell wall, a cell membrane, a nuclear membrane, a nucleoprotein, or combinations thereof and/or to denature the gDNA; (b) mixing into the solution resulting from step (a) a solution characterized by high salt and sufficient buffering capacity to reduce the pH of the solution to less than 10, thereby producing a neutralized solution; (c) centrifuging the sample at a speed and length of time sufficient to clarify the preparation; and (d) removing insoluble material from the neutralized and clarified preparation, whereby a solution of gDNA is produced.
    Type: Grant
    Filed: February 4, 2014
    Date of Patent: January 31, 2017
    Assignee: Syngenta Participations AG
    Inventor: Yanshan Ji
  • Patent number: 9486434
    Abstract: Provided is a composition that contains a mixture of broccoli seed and mustard seed. Before mixing the broccoli seed with the mustard seed, the broccoli seed is subjected to baking and a pressurized heat treatment. The broccoli seed is baked at a temperature of at least 200 degrees Fahrenheit for at least 60 minutes, and is subjected to a pressurized heat treatment of at least 200 degrees Fahrenheit at a pressure of at least 10 pounds/square inch for at least 5 minutes. Also provided is a method for therapy and/or prophylaxis of bladder cancer in an individual. The method entails administering orally to the individual a composition that contains an isothiocyanate (ITC) or a derivative thereof such that the administration inhibits the growth and/or recurrence of bladder cancer. Nutraceutical compositions are also provided.
    Type: Grant
    Filed: September 11, 2012
    Date of Patent: November 8, 2016
    Assignee: Health Research Inc.
    Inventors: Yuesheng Zhang, Arup Bhattacharya, Li Tang, Yun Li, Joseph D. Paonessa, Feng Geng, Yi Ding
  • Patent number: 9476071
    Abstract: Process for the enzymatic synthesis of the compound of formula (I): comprising enantioselective enzymatic hydrolysis of the nitrile of formula (IV): using the nitrilase of Rhodococcus rhodochrous of EMBL accession number EF467367.1, and the application of such a process in the synthesis of ivabradine and addition salts thereof with a pharmaceutically acceptable acid.
    Type: Grant
    Filed: February 26, 2014
    Date of Patent: October 25, 2016
    Assignee: LES LABORATOIRES SERVIER
    Inventors: Sandrine Pedragosa-Moreau, François Lefoulon
  • Patent number: 9415346
    Abstract: The present invention discloses a process for the recovery of betaines from electrodialysis (ED) waste streams, whereby said waste streams are treated with a pressure driven membrane process.
    Type: Grant
    Filed: February 3, 2010
    Date of Patent: August 16, 2016
    Assignee: Lonza Ltd.
    Inventors: Markus Bicker, Miguel Angel Caraucán Dávila
  • Patent number: 9404118
    Abstract: The invention relates to genetically modified Pichia ciferrii cells, to the use thereof and to a method of producing sphingoid bases and sphingolipids.
    Type: Grant
    Filed: July 23, 2012
    Date of Patent: August 2, 2016
    Assignee: EVONIK DEGUSSA GMBH
    Inventors: Tim Köhler, Christoph Schorsch, Eckhard Boles, Heiko Andrea, Mike Farwick, Steffen Schaffer
  • Patent number: 9388438
    Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
    Type: Grant
    Filed: July 15, 2015
    Date of Patent: July 12, 2016
    Assignee: Codexis, Inc.
    Inventors: Christopher K. Savile, Vesna Mitchell, Xiyun Zhang, Gjalt W. Huisman
  • Patent number: 9303277
    Abstract: The present invention relates to a microorganism having an improved ornithine-producing ability, in which the biosynthetic pathway of arginine form ornithine is blocked, the intracellular glutamate level is increased, and the biosynthetic pathway of ornithine from glutamate is enhanced, and a method for producing ornithine using the microorganism.
    Type: Grant
    Filed: December 8, 2011
    Date of Patent: April 5, 2016
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Hyang Choi, Kyoung Min Lee, Min Sun Kang, Sung Hoo Jhon, Hye Won Um, Su Jin Choi, Han Won Lee, Soo An Shin
  • Patent number: 9150890
    Abstract: The invention relates to genetically engineered Candida tropicalis cells, use thereof and a method of production of ?-hydroxycarboxylic acids and ?-hydroxycarboxylic acid esters.
    Type: Grant
    Filed: January 30, 2015
    Date of Patent: October 6, 2015
    Assignee: EVONIK DEGUSSA GmbH
    Inventors: Markus Poetter, Hans-Georg Hennemann, Steffen Schaffer, Thomas Haas
  • Patent number: 9121045
    Abstract: The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations.
    Type: Grant
    Filed: October 2, 2014
    Date of Patent: September 1, 2015
    Assignee: Codexis, Inc.
    Inventors: Christopher K. Savile, Vesna Mitchell, Xiyun Zhang, Gjalt W. Huisman
  • Patent number: 9115362
    Abstract: The present invention relates to a mutant microorganism having a high ability to produce cadaverine, and a method for producing cadaverine using the same. More specifically, the invention relates to a mutant microorganism having a high ability to produce cadaverine wherein a gene involved in the cadaverine degradation or utilization pathway is inactivated or deleted, and to a method for producing cadaverine in high yield by culturing the mutant microorganism under aerobic conditions. The mutant microorganism according to the present invention is useful for producing a high yield of cadaverine which can be widely used in various industrial applications.
    Type: Grant
    Filed: August 3, 2011
    Date of Patent: August 25, 2015
    Assignee: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Sang Yup Lee, Zhi Gang Qian, Xiaoxia Xia, Min Young Kim
  • Patent number: 9068175
    Abstract: Disclosed are preparations of modified interfacial enzymes, particularly lipases and phospholipases, immobilized on a solid support, wherein the enzyme is surrounded by hydrophobic microenvironment, thereby protected from deactivation and/or aggregation in the presence of hydrophilic agents, substrates and/or reaction products. The enzyme may be protected by being covalently bonded with lipid groups which coat the enzyme, or by being immobilized or embedded in a hydrophobic solid support. Also disclosed are processes for the preparation of the hydrophobically protected enzymes. The enzymes may be efficiently used in the preparation of biodiesel.
    Type: Grant
    Filed: May 7, 2008
    Date of Patent: June 30, 2015
    Assignee: Trans Bio-Diesel Ltd.
    Inventor: Sobhi Basheer
  • Publication number: 20150140617
    Abstract: The invention relates to genetically engineered Candida tropicalis cells, use thereof and a method of production of ?-hydroxycarboxylic acids and ?-hydroxycarboxylic acid esters.
    Type: Application
    Filed: January 30, 2015
    Publication date: May 21, 2015
    Applicant: EVONIK DEGUSSA GMBH
    Inventors: Markus POETTER, Hans-Georg Hennemann, Steffen Schaffer, Thomas Haas
  • Publication number: 20150132807
    Abstract: The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.
    Type: Application
    Filed: November 12, 2014
    Publication date: May 14, 2015
    Inventors: Nicholas J. Agard, Oscar Alvizo, Melissa Ann Mayo, Stefanie Ng Minor, James Nicholas Riggins, Jeffrey C. Moore
  • Publication number: 20150132808
    Abstract: A mutant lysine decarboxylase is produced by replacing at least one of the amino-acids in the amino acid sequence shown in sequence ID No. 4 of the sequence listing with another amino-acid.
    Type: Application
    Filed: January 17, 2013
    Publication date: May 14, 2015
    Inventors: Daisuke Mochizuki, Tadashi Araki, Akiko Natsuji, Tomomi Sakai, Tomonori Hidesaki
  • Patent number: 9029106
    Abstract: The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.
    Type: Grant
    Filed: June 18, 2013
    Date of Patent: May 12, 2015
    Assignee: Codexis, Inc.
    Inventors: Ish K. Dhawan, Gregory Miller, Xiyun Zhang
  • Patent number: 9023624
    Abstract: The present invention is related to a method for the fermentative production of hydroxymethionine, comprising the steps of: culturing a recombinant microorganism modified to produce methionine in an appropriate culture medium comprising a source of carbon, a source of sulfur and a source of nitrogen, recovering hydroxymethionine from the culture medium. In a specific embodiment, the recombinant microorganism is cultivated under conditions of nitrogen limitation. The invention is also related to the biologically-produced hydroxymethionine and its uses.
    Type: Grant
    Filed: December 30, 2010
    Date of Patent: May 5, 2015
    Assignee: Metabolic Explorer
    Inventors: Rainer Figge, Wanda Dischert
  • Publication number: 20150118721
    Abstract: The invention relates to novel enzymes that provide acetylated sphingoid bases.
    Type: Application
    Filed: March 13, 2013
    Publication date: April 30, 2015
    Applicant: EVONIK INDUSTRIES AG
    Inventors: Steffen Schaffer, Mike Farwick, Heiko Andrea, Tim Koehler, Daniel Wolff, Frank ter Veld, Ansgar Poetsch, Eckhard Boles, Christoph Schorsch
  • Publication number: 20150111262
    Abstract: This document describes biochemical pathways for producing glutaric acid, 5-aminopentanoic acid, 5-hydroxypentanoic acid, cadaverine or 1,5-pentanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C5 backbone substrate such as D-proline.
    Type: Application
    Filed: August 27, 2014
    Publication date: April 23, 2015
    Inventors: Adriana Leonora Botes, Alex Van Eck Conradie
  • Publication number: 20150111253
    Abstract: The invention relates to a method for oxidizing a fatty acid or an ester thereof of formula (I) H3C—(CH2)n-COOR, wherein R is selected from the group that comprises H, methyl, ethyl, propyl, and butyl, wherein n is 0 to 30, preferably 6 to 24, comprising the step of oxidizing the fatty acid or the ester thereof by contacting the fatty acid or the ester thereof with a cytochrome P450 monooxygenase of the CYP153 family in the presence of molecular oxygen and NAD(P)H and a whole-cell catalyst that expresses a recombinant cytochrome P450 monooxygenase of the CYP153 family, a recombinant alcohol dehydrogenase, a recombinant transaminase, and optionally one or more than one recombinant enzyme from the group comprising alanine dehydrogenase, ferredoxin, and ferredoxin reductase, and the use of said whole-cell catalyst to oxidize a fatty acid or an ester thereof.
    Type: Application
    Filed: March 12, 2013
    Publication date: April 23, 2015
    Applicant: EVONIK INDUSTRIES AG
    Inventors: Steffen Schaffer, Michaela Hauberg, Mirja Wessel, Hans-Georg Hennemann, Jan Christoph Pfeffer, Thomas Haas, Harald Haeger
  • Publication number: 20150104838
    Abstract: The present invention relates to a recombinant microorganism having enhanced ability of producing putrescine at high yield, which is generated by weakening the activity of NCgl1469 in a microorganism of Corynebacterium genus that is modified to produce putrescine, and a method for producing putrescine using the same.
    Type: Application
    Filed: January 11, 2013
    Publication date: April 16, 2015
    Inventors: Su Jin Choi, Hyang Choi, Min Sun Kang, Sung Hoo Jhon, Kyoung Min Lee, Hye Won Um, Young Lyeol Yang
  • Patent number: 8999680
    Abstract: The present invention relates to a novel method for the preparation of 1,4-diaminobutane [DAB]. The method according to the present invention involves at least one biocatalytic step which comprises the biocatalytic production of at least one N-protected precursor of DAB. The present invention also relates to a method for the preparation of DAB involving at least one biocatalytic step, and comprising the steps of a) biocatalytically preparing an N-protected precursor of DAB yielding a—biocatalytic reaction mixture containing the N-protected precursor of DAB, b) recovering the N-protected precursor from the biocatalytic reaction mixture and c) converting the N-protected precursor into DAB. More in particular, the present invention relates to a method for the preparation of DAB, wherein the at least N-protected precursor of DAB is selected from the group consisting of N5-protected ornithine, N-protected DAB, and N-protected 4-aminobutyraldehyde.
    Type: Grant
    Filed: July 20, 2010
    Date of Patent: April 7, 2015
    Assignee: DSM IP Assets B.V.
    Inventors: Liang Wu, Petronella Catharina Raemakers-Franken
  • Patent number: 8999681
    Abstract: A method of producing cadaverine is more efficient and at a higher yield than production methods by the conventional fermentation methods. The method includes culturing coryneform bacterium/bacteria having a resistance to a pH of 5.5 or less. Preferably, the coryneform bacterium/bacteria has/have lysine decarboxylase activity and, preferably, the coryneform bacterium/bacteria has/have homoserine auxotrophy and/or a resistance to S-(2-aminoethyl)-L-cysteine.
    Type: Grant
    Filed: December 8, 2011
    Date of Patent: April 7, 2015
    Assignee: Toray Industries, Inc.
    Inventors: Nanami Sasaki, Takashi Mimitsuka, Hideki Sawai, Kenji Sawai