Using Bacteria Patents (Class 435/170)
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Patent number: 8871266Abstract: Probiotic microorganisms are micro encapsulated by dispersing the probiotic microorganism in an aqueous suspension of a film forming protein and a carbohydrate; in an oil in water emulsion of a film forming protein and a carbohydrate and a fat; or in an oil which is subsequently dispersed in a film forming protein and a carbohydrate. The emulsion or suspension may be dried to form a powder. The probiotic may be dispersed in oil and then emulsified with the aqueous suspension and then dried to produce an encapsulated oil be dried to produce a powder. Oil suspended probiotics may be preferred where the probiotic is water sensitive. The preferred protein is casein or whey protein and the carbohydrate may be a resistant starch or a saccharide with a reducing sugar group. Where the probiotic is oxygen sensitive the protein carbohydrate is heated to create Maillard reaction products in the encapsulating film.Type: GrantFiled: March 30, 2004Date of Patent: October 28, 2014Assignee: Commonwealth Scientific & Industrial Research OrganisationInventors: Ross Crittenden, Luz Sanguansri, Mary Ann Augustin
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Patent number: 8865442Abstract: This invention relates to methods for producing isoprene by culturing recombinant cells (e.g., cells engineered to produce isoprene) under reduced oxygen inlet levels.Type: GrantFiled: December 21, 2012Date of Patent: October 21, 2014Assignees: Danisco US Inc., The Goodyear Tire & Rubber CompanyInventors: Gopal K. Chotani, Brian Kirshner, Jacob Latone, Jeff P. Pucci
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Patent number: 8859235Abstract: A method for the manufacture of products by biotechnological methods in bacterial cell culture is disclosed as well as products obtained, the use of certain additives to the media used in the manufacture of said products in bacterial cell culture media, and the use of said additives in reducing the detrimental effects of radicals in the manufacture of the products, as well as aspects related to these invention embodiments. The manufacturing process or method comprises adding one or more radical scavenging and/or antioxidative additive preferably selected from the group consisting of sterically hindered nitroxyls, sterically hindered hydroxylamines, sterically hindered hydroxylamine salt compounds, sterically hindered amino compounds and sterically hindered N-hydrocarbyloxyamines, benzofuranone compounds, as obligatory component(s) to the medium used during biosynthesis.Type: GrantFiled: August 11, 2010Date of Patent: October 14, 2014Assignee: BASF SEInventors: Franz Kaufmann, Trine Hefsgaard Miller, Johannes Flothmann, Beate Winter, Andreas Hafner
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Patent number: 8859249Abstract: A method for producing an iron (III) ion from the acidic solution containing an iodide ion and an iron (II) ion efficiently and stably is provided. The method including performing the following steps (a)-(b), repeatedly and continuously: (a) a step wherein the iron (II) ion in the acidic solution containing the iodide ion and the iron (II) ion is oxidized into iron (III) ion in a reactor using a microbes immobilizing carrier to which iron oxidizing microbes attached; (b) a step wherein sedimentation of the solution obtained in the step (a) is performed in a sedimentation tank to obtain the solution containing the iron (III) ion and concurrently the sediment of the microbes immobilizing carrier to which the iron oxidizing microbes have attached is recovered and then reintroduced into the reactor in the step (a).Type: GrantFiled: March 13, 2012Date of Patent: October 14, 2014Assignee: JX Nippon Mining & Metals CorporationInventors: Kenichi Kuwano, Akira Miura
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Patent number: 8852899Abstract: Embodiments of the invention relate to the enzymatic conversion of bioderived feedstocks to commercially valuable chemicals. The enzymatic conversions of the embodiments of the invention offer the potential for lower cost routes to these value-added chemicals. Some of the chemicals that are useful include nylon intermediates such as caprolactam, adipic acid, 1,6-hexamethylene diamine; butanediols such as 1,4-butanediol, 1,3-butanediol, and 2,3-butanediol; butanols such as 1-butanol, and 2-butanol; succinic acid, butadiene, isoprene, and 3-hydroxypropanoic acid.Type: GrantFiled: June 15, 2012Date of Patent: October 7, 2014Assignee: INVISTA North America S.a.r.l.Inventors: Paul S. Pearlman, Changlin Chen, Adriana L. Botes
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Patent number: 8852907Abstract: The efficient production of ethanol from low-cost biomass (e.g., corn, sugar beets, sugar cane, switchgrass and/or paper) has become increasingly important in making ethanol competitive with gasoline and decreasing the United States' dependence on foreign oil. For example, to reduce the cost of transporting biomass to ethanol production facilities, mobile systems for producing ethanol from biomass are provided. Also provided are small-scale ethanol production facilities. For example, instead of transporting biomass to the production facility, the facility is transported to the biomass or is located nearby the source of the biomass. The ethanol production facilities or components thereof may be transported via land, water, or air. Production of other products, such as hydrocarbons, natural gas, hydrogen gas, plastics, polymers, and proteins, can also be made by the methods and facilities. Any product described herein can be made in finished form or un-finished form and moved, e.g., to a fixed facility, e.g.Type: GrantFiled: February 5, 2013Date of Patent: October 7, 2014Assignee: Xyleco, Inc.Inventor: Marshall Medoff
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Publication number: 20140295520Abstract: This disclosure describes a cell that includes an engineered subcellular compartment that generally includes a proteinaceous shell that includes at least one bacterial microcompartment (BMC) polypeptide. In some embodiments, the engineered subcellular compartment can further include one or more targeted enzymes. We further describe methods of making and using such cells.Type: ApplicationFiled: October 25, 2012Publication date: October 2, 2014Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTAInventors: Claudia Margarete Schmidt-Dannert, Swati Choudhary Saurkar, Maureen Blacker Quin, Mark Anton Held
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Patent number: 8846082Abstract: An encapsulation system is provided comprising nitrogen-purge, instant bonding encapsulation method. Specifically, the encapsulation system comprises a composition, a two-piece capsule comprising a capsule cap and a capsule body; a gas to purge oxygen from the composition within the capsule; and a sealing solution to seal the capsule cap to the capsule body. Associated methods for encapsulating compositions using the encapsulation system are also provided.Type: GrantFiled: March 21, 2006Date of Patent: September 30, 2014Assignee: Vita-Herb Nutriceuticals, Inc.Inventor: Bing Baksh
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Patent number: 8841099Abstract: The present invention relates to the cell-based production of bacterial nonulosonates and their biosynthetic precursors. Specifically, the present invention provides recombinant cells for the production of pseudaminic acid, legionaminic acid, UDP-2,4-diacetamido-2,4,6-trideoxy-?-L-altropyranose, and UDP-2,4-diacetamido-2,4,6-trideoxy-?-D-glucopyranose. Methods for producing the sugars are also provided.Type: GrantFiled: April 20, 2011Date of Patent: September 23, 2014Assignees: National Research Council of Canada, University of OttawaInventors: Christopher N. Boddy, Susan M. Logan, Benjamin R. Lundgren, Ian C. Schoenhofen, Dennis M. Whitfield
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Patent number: 8835152Abstract: Compositions of peptides and surface-active agents are described, as are methods of making and using such compositions. The compositions are capable of affecting metabolic rates in biological systems, and to accelerate nutrient uptake without a concomitant increase in biofilm production.Type: GrantFiled: December 4, 2012Date of Patent: September 16, 2014Assignee: Advanced Biocatalytics CorporationInventor: Carl W. Podella
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Publication number: 20140227758Abstract: A method for the inerting of vessels used in the digestion of organic waste, the method characterised by the method steps of: (i) Capturing exhaust gas (14) from a chemical conversion means (12); (ii) Directing at least a portion of the captured exhaust gas (14) from step (i) to a vessel (40) used in the digestion of organic waste; and (iii) Purging the vessel (40) using the exhaust gas (14) so as to avoid an explosive mix of gases therein, wherein the exhaust gas (14) contains sufficient oxygen to prevent substantially the production of methane by anaerobic bacteria in the vessel (40).Type: ApplicationFiled: June 26, 2012Publication date: August 14, 2014Applicant: ANAECO LIMITEDInventors: Barry Antony Tindall, Gareth James Buckland
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Patent number: 8802079Abstract: A bacillus composition characterized by fast germination and outgrowth in bile salts (simulated gut environment) and by producing a compound of interest. The bacillus composition may be used as supplement in animal feed where it has a probiotic (health promoting) effect and increases the digestion and availability of nutrients from animal feeds.Type: GrantFiled: December 16, 2009Date of Patent: August 12, 2014Assignee: Chr. Hansen A/SInventors: Inge Knap, Ane Knarreborg, Thomas Dyrmann Leser, Lund Bente, Mette Dines Cantor, Patrick Derkx
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Patent number: 8785160Abstract: The invention provides methods and pharmaceutical compositions for treating symptoms associated with lactose intolerance and for overall improvement in gastrointestinal health. Described herein are methods and pharmaceutical compositions for improving overall gastrointestinal health or for decreasing symptoms of lactose intolerance by administering to subject in need thereof a pharmaceutical composition comprising a prebiotic, optionally in combination with effective amount of a probiotic microbe or microbes.Type: GrantFiled: April 28, 2011Date of Patent: July 22, 2014Assignee: Ritter Pharmaceuticals, Inc.Inventors: Andrew J. Ritter, Dennis Savaiano, David Barnes, Todd Klaenhammer
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Patent number: 8772003Abstract: A bacterial cellulose film is provided, including microbial fibers consisting of bacterial cellulose, wherein the microbial fibers are arranged in a density gradient in a direction of the bacterial cellulose film thickness.Type: GrantFiled: May 23, 2011Date of Patent: July 8, 2014Assignee: Nympheas International Biomaterial Corp.Inventors: Yi-Chuan Lin, Yuh-Chyun Wey, Mei-Ling Lee
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Patent number: 8771720Abstract: Polyhydroxyalkanoates (PHAs) from which pyrogen has been removed are provided. PHAs which have been chemically modified to enhance physical and/or chemical properties, for targeting or to modify biodegradability or clearance by the reticuloendothelial system (RES), are described. Methods for depyrogenating PHA polymers prepared by bacterial fermentation processes are also provided, wherein pyrogens are removed from the polymers without adversely impacting the polymers' inherent chemical structures and physical properties. PHAs with advantageous processing characteristics, including low melting points and/or solubility in non-toxic solvents, are also described. The PHAs are suitable for use in in vivo applications such as in tissue coatings, stents, sutures, tubing, bone, other prostheses, bone or tissue cements, tissue regeneration devices, wound dressings, drug delivery, and for diagnostic and prophylactic uses.Type: GrantFiled: July 10, 2012Date of Patent: July 8, 2014Assignee: Metabolix, Inc.Inventors: Simon F. Williams, David P. Martin, Tillman Gerngross, Daniel M. Horowitz
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Publication number: 20140186916Abstract: Methods and articles are provided for reducing the amount of water consumed by a plant over a period of time, sequestering CO2, and producing electricity, where each method includes providing the plant with a composition including at least about 0.1 (wt./wt. or vol./vol.) % CO2 and/or at least about 0.1 wt./wt. % of a composition that generates CO2. An apparatus is also disclosed for providing the plant with a composition including CO2 and/or a composition that generates CO2.Type: ApplicationFiled: December 28, 2012Publication date: July 3, 2014Applicant: Elwha LLCInventors: Paul G. Allen, Kenneth G. Caldeira, Bran Ferren, William Gates, W. Daniel Hillis, Roderick A. Hyde, Muriel Y. Ishikawa, Edward K.Y. Jung, Jordin T. Kare, John Latham, Nathan P. Myhrvold, Stephen H. Salter, Clarence T. Tegreene, David B. Tuckerman, Thomas A. Weaver, Charles Whitmer, Lowell L. Wood, JR., Victoria Y.H. Wood
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Patent number: 8765404Abstract: Some aspects of this invention relate to methods useful for the conversion of a carbon source to a biofuel or biofuel precursor using engineered microbes. Some aspects of this invention relate to the discovery of a key regulator of lipid metabolism in microbes. Some aspects of this invention relate to engineered microbes for biofuel or biofuel precursor production.Type: GrantFiled: March 2, 2011Date of Patent: July 1, 2014Assignee: Massachusetts Institute of TechnologyInventors: Gregory Stephanopoulos, Syed Hussain Imam Abidi
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Patent number: 8765421Abstract: A method for producing coenzyme Q10 by using stock culture from solid phase fermentation. The strain used in this method is Rhodobacter sphaeroides. Strain passaging is carried out by culturing in a slant medium. After steam cooking and air drying, solid medium is subpackaged and sterilized, wherein said solid medium includes solid components and liquid components. The fresh culture of Rhodobacter sphaeroides on the slant medium is added with sterile water, and the resultant bacterial suspension is added into the solid medium, cultured and used as stock culture for primary fermentation. The method of the present invention can enhance the fermentation level of coenzyme Q10, reduce the cascades of fermentation, shorten production cycle, simplify production processes, and lower production cost.Type: GrantFiled: April 26, 2011Date of Patent: July 1, 2014Assignee: Xiamen Kingdomway Group CompanyInventors: Jinqing Chen, Dajun Chen, Junhuang Chen, Meiqiong Wu, Songgang Su, Jianzhong Huang, Yi Zheng
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Publication number: 20140178961Abstract: The present invention relates to a construct for accomplishing fixation of carbon dioxide and/or reduction of carbon dioxide emission in a heterotrophic microorganism (for example, a heterotrophic fermentation strain, such as E. coli), a vector comprising the construct, a heterotrophic microorganism (for example, a heterotrophic fermentation strain, such as E. coli) comprising the construct or being transformed with the vector, and a method for fixing carbon dioxide and/or reducing carbon dioxide emission in a heterotrophic microorganism (for example, a heterotrophic fermentation strain, such as E. coli).Type: ApplicationFiled: December 19, 2013Publication date: June 26, 2014Applicant: SHELL OIL COMPANYInventors: Xuefeng LU, Liu YANG, Zhengxu GAO, Xiaoming TAN
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Patent number: 8753856Abstract: The present invention provides a stable and viable biocatalyst with high activity and operational stability and method of immobilization thereof. The immobilization process is based on the principle of entrapment of partially purified enzyme precipitate which is simultaneously aggregated by cross linking agent like glutaraldehyde and further entrapped in a combination of natural polymer like gelatin and synthetic polymer like polyvinyl alcohol with effective gelation under mild conditions of temperature and pH, resulting in a stable biocatalyst. The enzymes immobilized by the above process include Penicillin acylase from rE. coli RE III (pKA18), Novel Penicillin acylase from Achromobacter sp (CCM4834) expressed in rE. coli BL21 (pK1P1) CCM 7394 and rE. coli RE III (pKX1P1).Type: GrantFiled: November 14, 2008Date of Patent: June 17, 2014Assignee: Fermenta Biotech Ltd.Inventors: Anupama Datla, Rajasekar Vyasarayani Williams, Trupti Krishnakant Ashar, Pavel Kyslik, Stanislav Becka
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Patent number: 8748138Abstract: A method or process for producing polyhydroxyalkanoates (PHAs) in biomass. The process entails feeding an organic carbon containing substrate to biomass enriched in PHA accumulating bacteria. Particularly the process entails intermittently supplying the substrate to the biomass at least three separate times over a selected period. The object of the process is to produce PHA having a relatively high molecular weight, at least 400,000 g/mole. By controlling the frequency at which the substrate is supplied to the biomass and by feeding a sufficient amount of the substrate to the biomass, the method or process produces the PHA having the relatively high molecular weight.Type: GrantFiled: December 10, 2010Date of Patent: June 10, 2014Assignee: Veolia Water Solutions & Technologies SupportInventors: Alan Gideon Werker, Simon Olof Harald Bengtsson, Carl Anton Börje Karlsson
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Publication number: 20140154769Abstract: A production system includes a structure configured to house a light-activated biological pathway. The production system further includes an optical filter attached to the structure. The optical filter is configured to receive light, to reflect a first portion of the received light, and to transmit a second portion of the received light, wherein the first portion has a different wavelength from the second portion. The production system is further configured to position the light-activated biological pathway to receive the second portion of the receive light.Type: ApplicationFiled: December 3, 2013Publication date: June 5, 2014Applicant: Wave Tech, LLCInventors: Matteo del Ninno, Jacob A. Bertrand
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Patent number: 8735135Abstract: Disclosed is a means for improving the poor conversion efficiency in a conventional bioconversion system using a transformant which is given by introducing a gene originated from xerogenic organisms. A transformant is prepared by using a host which is defective in a gene encoding a multidrug efflux protein and introducing a gene originated from xerogenic organisms. Use of the transformant results in much effective microbial conversion of a hydrophobic or amphipathic substrate compound into a desired compound. In case, an Escherichia coli is used as the host, the gene encoding a multidrug efflux protein to be defective may be tolC, acrA, acrB and the like.Type: GrantFiled: February 28, 2008Date of Patent: May 27, 2014Assignee: Microbiopharm Japan Co., LtdInventors: Tadashi Fujii, Atsushi Ochiai, Masashi Ito, Hiroki Kabumoto, Yoshikazu Fujii, Kazuhiro Machida
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Patent number: 8728782Abstract: Disclosed is a method of converting butyric acid contained in a fermentation broth into biofuel. This chemical conversion method includes separating biohydrogen from gases generated in the course of production of butyric acid through fermentation of carbohydrate, extracting butyric acid from the broth using an insoluble solvent, esterifying butyric acid thus producing butylbutyrate, and hydrogenolyzing all or part of butylbutyrate, thus obtaining butanol. Thereby, biobutanol can be efficiently and economically produced, and butylbutyrate, which has oxidation stability superior to that of conventional biodiesel (fatty acid methyl ester) and is thus regarded as novel biofuel, can be produced together.Type: GrantFiled: March 27, 2009Date of Patent: May 20, 2014Assignees: SK Innovation Co., Ltd., Korea Institute of Science and TechnologyInventors: Sin Young Kang, Cher Hee Park, Young Seek Yoon, In Ho Cho, Hyung Woong Ahn, Sam Ryong Park, Jong Hee Song, Seong Ho Lee, Byoung In Sang, Young Woong Suh, Young Soon Um, Sun Mi Lee
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Patent number: 8722374Abstract: The invention provides methods of producing secondary metabolites and under recombinant products oxygen-limited or anaerobic culture conditions. The methods include providing a porous substrate having a first side and a second side and which has a biofilm of microorganisms attached to the first side thereof, and causing a nutrient solution to flow through the biofilm and the substrate in a direction from the first side thereof to the second side thereof under oxygen-limited or anaerobic culture conditions. The microorganisms include Clostridium sp., Bacillus sp., Pseudomonas sp. Vibrio sp., Serratia sp., Rhodopseudomonas sp., Anaeromyxobacter sp., Desulphovibrio sp., and Candida sp., Lactococcus lactis, Escherichia coli, Bacillus subtillus, Pichia sp., Candida sp., Hansenula polymorpha and Saccharomyces cerevisiae.Type: GrantFiled: June 30, 2006Date of Patent: May 13, 2014Assignee: Synexa Life Science (Proprietary) LimitedInventors: Sheena Janet Fraser, Wade Edwards, P. G. Lourens
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Patent number: 8722035Abstract: Disclosed are formulations for enhancing the in vivo colonization of probiotic microorganisms that include digestive enzymes and probiotic microorganisms, and polysorbate surfactants. The enzymes include lactogenic enzyme formulations that promote growth of Lactobacillus probiotics, bifidogenic enzyme formulations that promote growth of Bifidobacterium probiotics and combination formulations that benefit both types of probiotics. It has been discovered that certain polysorbate surfactants, including polysorbate-60 and polysorbate-80, further promote probiotic microorganism growth, when used with the enzyme formulations. The formulations are preferably compounded as dry powders, to avoid water reaction with the enzymes in blended formulations. Such formulations can be contained in capsules, tablets, packets or bottles and administered orally, either sequentially or in one combined formulation.Type: GrantFiled: October 6, 2011Date of Patent: May 13, 2014Assignee: Master Supplements, Inc.Inventor: Randolph S. Porubcan
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Patent number: 8722373Abstract: Contamination was controlled in fermentations using Zymomonas mobilis as the biocatalyst, without negative impact on fermentation production, by the addition of virginiamycin. The effective concentration of virginiamycin was found to be dependent upon the type of fermentation medium used.Type: GrantFiled: December 10, 2013Date of Patent: May 13, 2014Assignee: E I du Pont de Nemours and CompanyInventors: Maria C Leana, Brian G Lefebvre
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Patent number: 8715650Abstract: The present invention relates to a composition for bath additives characterized in that the composition is obtained by a process comprising: subjecting herbal materials to liquid phase fermentation with Bacillus subtilis to obtain a culture, the herbal materials comprising 10% to 25% by weight of Zizania latifolia, 10% to 20% by weight of Artemisia capillaries, 5% to 15% by weight of Houttuynia cordata, 1% to 20% by weight of Saururus chinensis, 1% to 15% by weight of Leonurus sibiricus, 1% to 15% by weight of Angelica gigas, 1% to 15% by weight of Coix lachryma-jobi, 1% to 10% by weight of Plantago asiatica and 1% to 10% by weight of Rhododendron brachycarpum, based on a total dry weight of the herb materials; separating sludge from the resulting culture; and drying, sterilizing and pulverizing the sludge.Type: GrantFiled: June 10, 2011Date of Patent: May 6, 2014Inventor: Dong-Joo Lee
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Patent number: 8709752Abstract: The present invention provides a process for producing a dipeptide or a dipeptide derivative using a phosphate donor, a substance selected from the group consisting of adenosine-5?-monophosphate, adenosine-5?-diphosphate and adenosine-5?-triphosphate, one or more kinds of amino acids or amino acid derivatives, and as enzyme sources, a protein having polyphosphate kinase activity, or a culture of cells having the ability to produce the protein or a treated matter of the culture, and a protein having the activity to ATP-dependently form the dipeptide or dipeptide derivative from one or more kinds of amino acids or amino acid derivatives, or a culture of cells having the ability to produce the protein or a treated matter of the culture.Type: GrantFiled: March 27, 2012Date of Patent: April 29, 2014Assignee: Kyowa Hakko Bio Co., Ltd.Inventors: Shin-ichi Hashimoto, Hajime Ikeda, Makoto Yagasaki
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Patent number: 8691974Abstract: A novel BC fermentation technique for controlling 3D shape, thickness and architecture of the entangled cellulose nano-fibril network is presented. The resultant nano-cellulose based structures are useful as biomedical implants and devices, are useful for tissue engineering and regenerative medicine, and for health care products. More particularly, embodiments of the present invention relate to systems and methods for the production and control of 3-D architecture and morphology of nano-cellulose biomaterials produced by bacteria using any biofabrication process, including the novel 3-D Bioprinting processes disclosed. Representative processes according to the invention involve control of the rate of production of biomaterial by bacteria achieved by meticulous control of the addition of fermentation media using a microfluidic system. In exemplary embodiments, the bacteria gradually grew up along the printed alginate structure that had been placed into the culture, incorporating it.Type: GrantFiled: September 28, 2010Date of Patent: April 8, 2014Assignee: Virginia Tech Intellectual Properties, Inc.Inventors: Paul Gatenholm, Henrik Backdahl, Theodore Jon Tzavaras, Rafael V. Davalos, Michael B. Sano
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Publication number: 20140087439Abstract: The present invention provides a complex of a metal reducing substance and a culture medium component, which is produced in the culturing process of reducing bacteria and the use thereof. The complex of the present invention is a complex produced in the culturing process of reducing bacteria and a compound of a metal reducing substance and a culture medium component. The complex is an adsorbent for quickly and efficiently adsorbing ionic substances present in a solution, and also functions as a reducing agent for reducing specific precious metal ions or platinum-group metal ions.Type: ApplicationFiled: May 23, 2012Publication date: March 27, 2014Applicant: AISIN SEIKI KABUSHIKI KAISHAInventors: Isao Otsubo, Yasuhiro Konishi, Norizo Saito
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Patent number: 8679823Abstract: The present invention relates to the use of F4+ non-pathogenic Escherichia coli strains to promote growth in an animal. The present invention also relates to the use of such strains to homogenize growth among a herd of animals. More specifically, the animal(s) of interest in the present invention are those wherein growth promotion or growth homogenization are desired goals, such as animals reared for meat production. The present invention further relates to a method for promoting growth of an animal as well as a method for homogenizing growth among a herd of animals.Type: GrantFiled: November 30, 2012Date of Patent: March 25, 2014Assignee: Valorisation-Recherche, Limited PartnershipInventors: Eric Nadeau, John Morris Fairbrother
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Patent number: 8679797Abstract: The present invention provides a method for extracting glucan and mannan from the cell wall of a microorganism. Specifically, the method of the present invention in one embodiment comprises the steps of: a) treating the cells of the microorganism with an alkaline protease and an mannanase; b) separating the mixture from step a) into a heavy phase and a light phase; c) drying the heavy phase obtained from step b), obtaining the glucan preparation; and d) drying the light phase obtained from step b), obtaining the mannan preparation. Optionally, in the step c), the heavy phase obtained from step b) may be treated sequentially with an alkali and an acid, and separated again into a heavy phase and a light phase. The heavy phase is dried, obtaining the glucan preparation. The present invention further relates to the glucan preparation and mannan preparation produced thereby, and the uses thereof.Type: GrantFiled: December 31, 2008Date of Patent: March 25, 2014Assignee: Angel Yeast Co., Ltd.Inventors: Xuefeng Yu, Zhihong Li, Minghua Yu, Juan Yao, Yan Zhang
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Publication number: 20140080176Abstract: The invention provides an organism for expressing foreign DNA, the organism engineered to accept standard DNA carriers. The genome of the organism codes for intracytoplasmic membranes and features an interruption in at least one of the genes coding for restriction enzymes. Further provided is a system for producing biological materials comprising: selecting a vehicle to carry DNA which codes for the biological materials; determining sites on the vehicle's DNA sequence susceptible to restriction enzyme cleavage; choosing an organism to accept the vehicle based on that organism not acting upon at least one of said vehicle's sites; engineering said vehicle to contain said DNA; thereby creating a synthetic vector; and causing the synthetic vector to enter the organism so as cause expression of said DNA.Type: ApplicationFiled: September 14, 2012Publication date: March 20, 2014Applicant: UCHICAGO ARGONNE, LLCInventors: Philip D. Laible, Deborah K. Hanson
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Patent number: 8673598Abstract: The present invention relates to bacterial strains, capable of utilizing glycerol as a carbon source for the fermentative production of succinic acid, wherein said strains are genetically modified so that they comprise a deregulation of their endogenous pyruvate-formate-lyase enzyme activity, as well as to methods of producing organic acids, in particular succinic acid, by making use of such microorganism. The present invention also relates to the downstream processing of the produced organic acids by cation exchange chromatography.Type: GrantFiled: February 12, 2010Date of Patent: March 18, 2014Assignee: BASF SEInventors: Hartwig Schroder, Stefan Haefner, Gregory Von Abendroth, Rajan Hollmann, Aline Raddatz, Hansgeorg Ernst, Hans Gurski
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Patent number: 8669093Abstract: A method for producing D-lactic acid in high yield, and to provide a method for producing D-lactic acid with high selectivity, in which optical purity is high and a by-product organic acid is small. In one aspect, a microorganism, wherein activity of pyruvate formate-lyase (pfl) is inactivated or decreased, and further activity of Escherichia coli-derived NADH-dependent D-lactate dehydrogenase (ldhA) is enhanced, is cultured to efficiently produce D-lactic acid. With regard to a method for enhancing ldhA activity, by linking, on a genome, a gene encoding ldhA with a promoter of a gene which controls expression of a protein involved in a glycolytic pathway, a nucleic acid biosynthesis pathway or an amino acid biosynthesis pathway, suitable results are obtained compared to the method for enhancing expression of the gene using an expression vector.Type: GrantFiled: September 17, 2004Date of Patent: March 11, 2014Assignee: Mitsui Chemicals, Inc.Inventors: Mitsufumi Wade, Toshihiro Oikawa, Daisuke Mochizuki, Junko Tokuda, Miyuki Kawashima, Tadashi Araki, Reiko Abe, Hitoki Miyake, Hitoshi Takahashi, Hideki Sawai, Takashi Mimitsuka, Takashi Morishige, Yosuke Higashi
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Patent number: 8658408Abstract: The invention provides method for producing butanediol and specifically 2,3-butanediol by anaerobic fermentation of a gaseous substrate comprising CO by a culture of at least one micro-organism. In accordance with particular methods of the invention, 2,3-butanediol is produced by anaerobic fermentation of substrates including carbohydrates and carbon monoxide. The invention further provides for the up-regulation of a native 2,3-butanediol dehydrogenase gene in the at least one micro-organism. The 2,3-butanediol can be further converted to compounds such as butene(s), butadiene and methyl ethyl ketone. These compounds can be further converted to chemical products such as 2-butanol.Type: GrantFiled: February 26, 2013Date of Patent: February 25, 2014Assignee: Lanza Tech New Zealand LimitedInventors: Sean Dennis Simpson, Loan Phuong Tran, Christophe Daniel Mihalcea, Jennifer Mon Yee Fung, FungMin Liew, Shane Fleming
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Patent number: 8652549Abstract: The invention relates to a post-extraction process for preparing low acyl gellan gum having high solubility property, the process comprising the following steps: (1) enzyme treatment of fermentation broth; (2) flocculation of fermentation broth; (3) deacylation treatment; (4) clarification treatment of deacylated solution; (5) dehydration treatment of clarified and deacylated gellan gum solution; (6) ion exchange and decoloration treatment; and (7) drying and milling. The process according to the invention produces a product, which has largely improved solubility, largely improved quality, good appearance, high transparence, and high gel strength.Type: GrantFiled: June 2, 2010Date of Patent: February 18, 2014Assignee: Zhejiang DSM Zhongken Biotechnology Co., Ltd.Inventors: Rongming Wu, Yanfei Zhong, Qi Guo, Yueqiang Shen, Liping Shen
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Patent number: 8652490Abstract: The subject invention provides a novel and advantageous strain of Pasteuria bacteria with nematicidal activity against Reniform nematodes. The subject invention provides the novel bacteria culture referred to as ATCC PTA-9643, and mutants thereof. Also provided are nematicidal compositions comprising the Pasteuria strain or its mutants or variants and methods for treating phytopathogenic and soil-dwelling nematodes.Type: GrantFiled: January 26, 2010Date of Patent: February 18, 2014Assignee: Pasteuria Bioscience, Inc.Inventors: Thomas E. Hewlett, John P. Waters
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Patent number: 8642305Abstract: A bacillus composition characterized by fast germination and outgrowth in bile salts (simulated gut environment) and by high-level secretion of phytase. The bacillus composition may be used as supplement in animal feed where it has a probiotic (health promoting) effect and increases the digestion and availability of nutrients from animal feeds.Type: GrantFiled: November 14, 2012Date of Patent: February 4, 2014Assignee: Chr. Hansen A/SInventors: Inge Knap, Ane Knarreborg, Thomas Dyrmann Leser, Bente Lund
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Publication number: 20140017724Abstract: The present invention relates to compositions and methods to identify novel bacteria and metabolites derived therefrom. More specifically, the invention describes a novel method to isolate bacteria producing metabolites of interest from environmental samples. Particularly, the invention discloses a method to select rare antibiotic producing bacteria. The invention can be used from any sample and allows the isolation of bacteria having e.g., pharmaceutical or agrochemical interest.Type: ApplicationFiled: September 18, 2013Publication date: January 16, 2014Applicants: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, DEINOVEInventors: JEAN-PAUL LEONETTI, STÉPHANIE TEXIER
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Publication number: 20140011706Abstract: The present invention relates to a method for the display of recombinant functional polypeptides containing a prosthetic group selected from metal porphyrin and flavin containing groups on the surface of a host cell using the transporter domain of an autotransporter.Type: ApplicationFiled: September 22, 2011Publication date: January 9, 2014Applicant: Autodisplay Biotech GMBHInventors: Stephanie Schumacher, Rita Bernhardt, Frank Hannemann, Joachim Jose
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Publication number: 20140011244Abstract: A method for producing naturally derived beneficial compounds including dispersing a microbiological culture media including at least one live probiotic organism, and at least one nutraceutical and/or at least one nutritive agent in distilled water to form a broth, incubating the broth at a predetermined temperature for a select period of time to induce probiotic activity; halting the probiotic activity, and separating the desired compound from the broth.Type: ApplicationFiled: September 9, 2013Publication date: January 9, 2014Applicant: Premier Research Labs, LPInventor: Robert J. Marshall
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Publication number: 20140004582Abstract: The present invention relates to producing chemicals and biofuels from wood material, e.g. mixed forest biomass. Specifically, the invention concerns a process for conditioning spent liquor produced by SO2-ethanol-water (SEW) fractionation of wood chips for fermentation to butanol, ethanol and acetone/isopropanol (so called ABE process) by Clostridia bacteria.Type: ApplicationFiled: March 16, 2012Publication date: January 2, 2014Applicant: API Intellectual Property Holdings, LLCInventors: Adriaan Van Heiningen, Evangelos Sklavounos
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Publication number: 20140004578Abstract: The invention generally relates to methods, devices, and microbes involved in performing redox imbalanced fermentations. In one aspect, the invention provides a device that generally includes an electrode and at least one microbe in electron communication with the electrode. The microbe generally can exhibit increased activity of at least one enzyme involved in converting a substrate to a redox imbalanced product. In another aspect, the invention provides a method for performing redox imbalanced fermentation. Generally, the method includes providing a substrate to a microbe under conditions effective for the microbe to metabolize the substrate to a redox imbalanced product. At least one microbe may be in contact with an electrode. In some cases, metabolic conversion of the substrate to the redox imbalanced product can include transferring electrons between the electrode and the microbe.Type: ApplicationFiled: September 13, 2011Publication date: January 2, 2014Inventors: Daniel R. Bond, Jeffrey A. Gralnick, Daniel E. Ross, Jeffrey M. Flynn
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Patent number: 8617862Abstract: The invention provides non-naturally occurring microbial organisms having a propylene pathway. The invention additionally provides methods of using such organisms to produce propylene.Type: GrantFiled: June 19, 2012Date of Patent: December 31, 2013Assignee: Genomatica, Inc.Inventors: Anthony P. Burgard, Robin E. Osterhout, Jun Sun, Priti Pharkya, Mark J. Burk
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Publication number: 20130344558Abstract: Wheat bran culture mix is used to inoculate organic waste and produce an inoculated waste material, where the wheat bran culture mix includes soil derived microbes. The inoculated waste material is shredded to produce shredded inoculated waste material which is fermented the shredded inoculated waste material for at least 7 days. Contents from the fermenter are then transferred into a dewatering device to produce dewatered contents which are then separated into soluble and suspended products.Type: ApplicationFiled: March 9, 2012Publication date: December 26, 2013Applicant: BOKASHICYCLE NVC LLCInventor: Lawrence R. Green
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Publication number: 20130330787Abstract: A method produces a chemical through continuous fermentation including: (a) culturing a cell in a culture medium in a fermentor to ferment a feedstock to produce a chemical; (b) conducting filtration of the culture medium with a separation membrane module; (c) separating a permeate containing the chemical from the culture medium while retaining a non-permeated liquid in the fermentor, and (d) supplying a gas from at least one of a lower portion of the separation membrane module and a pipe communicating between the fermentor and the separation membrane module to adjust a gas linear velocity in the separation membrane module to 0.15 cm/s to 70 cm/s while supplying the separation membrane module with a liquid.Type: ApplicationFiled: December 8, 2011Publication date: December 12, 2013Inventors: Satoko Kanamori, Jihoon Cheon, Takashi Mimitsuka, Norihiro Takeuchi, Makoto Nishida, Yuji Tanaka
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Publication number: 20130330792Abstract: A method of producing a chemical by continuous fermentation includes a fermentation step of converting a fermentation feedstock to a fermentation liquid containing a chemical by fermentation on cultivation of a microorganism; a membrane separation step of recovering the chemical as a filtrate by a separation membrane from the fermentation liquid; a concentrating step of obtaining a permeate and a concentrate containing the chemical by a reverse osmosis membrane from the filtrate; and/or a purification step of distilling the filtrate to increase a purity of the chemical, in which, cleaning etc. of the separation membrane in the membrane separation step is preformed by using the permeated liquid from the reverse osmosis membrane in the concentrating step and/or the condensed liquid in the purification step.Type: ApplicationFiled: September 15, 2011Publication date: December 12, 2013Applicant: Toray Industries, Inc.Inventors: Norihiro Takeuchi, Masahiro Henmi, Jihoon Cheon, Masateru Ito, Hiroshi Hayakawa, Makoto Nishida
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Publication number: 20130324406Abstract: A system and method for the production of microbial consortiums and by-product material is provided. A physical containment system comprising phase spaces arranged in a discrete order to favor specific biological reactions is also provided. Phase profiles and phase data sets include the pre-determined physical and biological parameters for the phase space transitions. Movement of material from one phase to the next is hydraulically balanced enabling working fluid to continuously move in a fixed direction and rate of flow. Continuous monitoring of phase profiles and phase data sets provide feedback to the system enabling alteration of the conditions in the system to control reactions therein.Type: ApplicationFiled: March 15, 2013Publication date: December 5, 2013Inventors: Robert Chisholm, Michael LaMontagne, Robert N. Ames, David P. Lanciault, John R. Coyne