Multi-enzyme System Patents (Class 435/175)
  • Patent number: 9983211
    Abstract: The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.
    Type: Grant
    Filed: April 25, 2016
    Date of Patent: May 29, 2018
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Thomas Diller, Bethany Sutton, George Hanson, Timothy Updyke
  • Patent number: 9885034
    Abstract: Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.
    Type: Grant
    Filed: September 22, 2014
    Date of Patent: February 6, 2018
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: Serge Saxonov
  • Patent number: 9868973
    Abstract: A method for optimizing post-translational modifications of recombinant proteins expressed in living cells is described. More particularly, a method for modulation of host proteins in living cells that control PTMs on recombinant proteins is described that has particularly useful applications in developing manufacturing process changes or in biosimilar development. The goal of this modulation is to produce a recipe for production of a recombinant protein in the new process or in the biosimilar that will produce a targeted PTM profile in the resulting protein product. In the method one or more modulators are selected, as from a modulator library, which affect the activity of host proteins. These modulators are added to media during production such that the resulting product matches the PTMs of the reference product.
    Type: Grant
    Filed: January 13, 2015
    Date of Patent: January 16, 2018
    Assignee: STC Biologics, Inc.
    Inventors: Magdalena Leszcyniecka, Sergey Shulga-Morskoy
  • Patent number: 9719987
    Abstract: The present invention relates to methods for determining the probability of transplant rejection based on the determination in the subject recipient of the transplant of the levels of antibodies specific for hyaluronic acid. The invention relates as well to methods for attenuating transplant rejection and compositions to prevent transplant rejection based on the depletion of anti-hyaluronic acid antibodies from the subject.
    Type: Grant
    Filed: August 12, 2013
    Date of Patent: August 1, 2017
    Assignee: FUNDACIO INSTITUT D'INVESTIGACIO BIOMEDICA DE BELLVITGE (IDIBELL)
    Inventor: Rafael Ma├▒ez Mendiluce
  • Patent number: 9540631
    Abstract: Compositions are provided to immobilize and protect oxidase enzymes from proteolytic and hydrolytic attack, utilizing a porous, inert organic, or inorganic, substrate matrix useful in generating hydrogen peroxide in situ for use in oral, topical mucosal and intracavity uses for use in or on the human body and other mammalian, reptile, fish and amphibians. The selected porosity of the support matrix provides the basis for generating hydrogen peroxide on a steady state basis. Both diatomaceous earth and silica gel, either in natural or pre-silanized forms, allow for the excluding of non-preferred enzymes from the support matrix, and for diffusion of the peroxide in the reduction of oral pathogens and their attendant malodorous compounds, or other pathogens in the mucosa, bodily fluids and cavities, in addition to oxidizing color bodies to affect bleaching.
    Type: Grant
    Filed: July 10, 2008
    Date of Patent: January 10, 2017
    Inventors: Peter T. Pugliese, Steven M. Pugliese, Joseph A. Ehrhard
  • Publication number: 20150122646
    Abstract: The present disclosure generally relates to devices and procedures for the development of glucose oxidase-bound electrodes by a covalent binding of glucose oxidase on amine-functionalized electrodes. More particularly, the present disclosure is related to covalently-bound enzyme-coated electrodes that are leach-proof and highly stable for continuous glucose monitoring. The glucose oxidase-bound electrodes are employed for the development of a mediator-less electrochemical glucose sensing procedure having no interference from biological substances and drugs.
    Type: Application
    Filed: May 3, 2013
    Publication date: May 7, 2015
    Inventors: Khalid Ali Al-Rubeaan, Dan Zheng, Fwu-Shan Sheu, Sandeep Kumar Vashist
  • Patent number: 8785151
    Abstract: Disclosed herein is pyrroline-carboxy-lysine (PCL), a pyrrolysine analogue, which is a natural, biosynthetically generated amino acid, and methods for biosynthetically generating PCL. Also disclosed herein are proteins, polypeptides and peptides that have PCL incorporated therein and methods for incorporating PCL into such proteins, polypeptides and peptides. Also disclosed herein is the site-specific derivatization of proteins, polypeptides and peptides having PCL or pyrrolysine incorporated therein. Also disclosed herein is the crosslinking of proteins, polypeptides and peptides having PCL or pyrrolysine incorporated therein.
    Type: Grant
    Filed: October 23, 2009
    Date of Patent: July 22, 2014
    Inventors: Bernhard Geierstanger, Weijia Ou, Susan E. Cellitti, Tetsuo Uno, Tiffany Crossgrove, Hsien-Po Chiu, Jan Grunewald, Xueshi Hao
  • Patent number: 8765472
    Abstract: The present invention relates to methods and devices to obtain multicellular arrangements in stable, stationary and reproducible spatial configuration, and optionally with controlled internal cell organization, methods for preparing such devices, methods for studying the cells' shapes, the cells' architectures, the cells' mechanical equilibrium, the cell-cell interaction, the cell movement and migration, the cell differentiation, the global internal cells' organizations, the cells' polarities and division, and/or any function of cells, methods for screening compounds of interest which enhance or inhibit specific cell functions.
    Type: Grant
    Filed: October 23, 2009
    Date of Patent: July 1, 2014
    Assignee: Commissariat a l'Energie Atomique et aux Energies Alternatives
    Inventor: Manuel Thery
  • Patent number: 8759055
    Abstract: A fuel cell is provided with an anode and a cathode. The anode is in electrical communication with an anode enzyme and the cathode is in electrical communication with a cathode enzyme. The anode enzyme is preferably an oxidase or a dehydrogenase. The cathode enzyme is a copper-containing enzyme, such as a lacasse, an ascorbate oxidase, a ceruloplasmine, or a bilirubin oxidase. Preferably, the cathode enzyme is operable under physiological conditions. Redox polymers serve to wire the anode enzyme to the anode and the cathode enzyme to the cathode. The fuel cell can be very small in size because it does not require a membrane, seal, or case. The fuel cell can be used in connection with a biological system, such as a human, as it may operate at physiological conditions. By virtue of its size and operability at physiological conditions, the fuel cell is of particular interest for applications calling for a power source implanted in a human body, such as a variety of medical applications.
    Type: Grant
    Filed: January 25, 2008
    Date of Patent: June 24, 2014
    Assignee: Abbott Diabetes Care Inc.
    Inventors: Adam Heller, Nicholas Mano, Hyug-Han Kim, Yongchao Zhang, Fei Mao, Ting Chen, Scott C. Barton
  • Publication number: 20140134700
    Abstract: Provided are nanocomplexes having at least two different enzymes and a polymeric network anchored to at least one of the enzymes. In some embodiments, the activities of the enzymes catalyze a cascade reaction.
    Type: Application
    Filed: July 6, 2012
    Publication date: May 15, 2014
    Applicant: The Regents of the University of California
    Inventors: Yunfeng Lu, Ming Yan, Yang Liu
  • Patent number: 8691545
    Abstract: Bone cages are disclosed including devices for biocompatible implantation. The structures of bone are useful for providing living cells and tissues as well as biologically active molecules to subjects.
    Type: Grant
    Filed: October 16, 2012
    Date of Patent: April 8, 2014
    Assignee: The Invention Science Fund I, LLC
    Inventors: Ed Harlow, Edward K. Y. Jung, Robert Langer, Eric C. Leuthardt, Lowell L. Wood, Jr.
  • Patent number: 8679755
    Abstract: A first cell mass substantially containing only either one of mesenchymal cells or epithelial cells and a second cell mass substantially containing only the other one of the cells are positioned in contact with each other inside a support carrier which can maintain a condition of cell contact; and cultured to obtain a tooth having a specific cell placement. Preferably, after the culturing, the support carrier having both cell masses is cultured with kidney cells.
    Type: Grant
    Filed: December 18, 2012
    Date of Patent: March 25, 2014
    Assignee: Organ Technologies Inc.
    Inventors: Takashi Tsuji, Kazuhisa Nakao
  • Publication number: 20130345655
    Abstract: A sanitary article is provided that includes a dried polymeric matrix with embedded enzyme. The presence of the enzyme in the polymeric matrix allows the sanitary article to absorb a bodily discharge fluid thereby allowing the embedded enzyme to function in the production of antibacterial compounds or by directly reducing the presence of viable bacteria in the discharge fluid. The enzyme activity promotes reduced odor in the sanitary article.
    Type: Application
    Filed: June 21, 2012
    Publication date: December 26, 2013
    Applicants: Toyota Motor Corporation, Toyota Motor Engineering & Manufacturing North America, Inc.
    Inventors: Songtao Wu, Hongfei Jia, Masahiko Ishii, Minjuan Zhang
  • Publication number: 20130316430
    Abstract: A PVC surface co-immobilized with the multiple enzymes for removal of stains useful in the field of washing or cleaning cloth and other household textile such as towels and sheets. The present invention also provides a process of preparation of the PVC surfaces and using such PVC surface. The PVC surface co-immobilized with the enzymes is useful as cheap and reusable alternative for washing of cloths.
    Type: Application
    Filed: December 7, 2011
    Publication date: November 28, 2013
    Applicant: MAHARSHI DAYANAND UNIVERSITY
    Inventors: Chandra Shekhar Pundir, Nidhi Chauhan
  • Publication number: 20130309745
    Abstract: Provided are a porous structure for forming anti-fingerprint coating capable of providing a self-cleaning function to a surface of a substrate, a method of forming anti-fingerprint coating using the same, an anti-fingerprint coated substrate prepared by the same method, and a product including the same. When the porous structure including a lipolytic enzyme is formed on the surface of the substrate, contaminants decomposed by an enzyme are absorbed into a pore, and thus anti-fingerprint coating may be more effectively performed to remove detectable contamination from a surface of the substrate. As a result, contamination by fingerprints on the surface of a display device, the appearance of an electronic device, or building materials can be effectively reduced.
    Type: Application
    Filed: July 19, 2013
    Publication date: November 21, 2013
    Inventors: Eun Jeong Lee, Hyeon Choi, Taek Ho Yang, Young Jun Hong
  • Patent number: 8574880
    Abstract: This invention relates to a method of immobilizing biocatalysts including protein and cells by co-precipitation with silicate or organosilicate matrices through the action of an organic template molecule. The organic template molecule is in general a polyamine such as polyethylenimine (PEI), or polypeptide compound bearing at least two or three basic residues selected from the group consisting of lysine, arginine, histidine, proline, hydroxyproline, N-methylhistidine, ornithine, taurine, ?-hydroxylysine, and ?-hydroxy-?-N,N,N trimethyllysine. The invention is also directed to a silica biocomposite comprising co-precipitates of active biocatalysts, silica or organosilicates, and an N-containing organic template molecule. Such silica biocomposites are useful in biocatalysis, and other applications requiring an immobilized biocatalyst. Preferred biocatalysts for this invention are enzymes and whole cells.
    Type: Grant
    Filed: February 27, 2009
    Date of Patent: November 5, 2013
    Assignee: Danisco US, Inc.
    Inventors: Risha Bond, Michael C. Jewett, Joseph C. McAuliffe, Donald E. Ward, II
  • Patent number: 8563245
    Abstract: A method that modifies surface properties of a substrate by manipulating the immobilized biomolecules in mild biological condition. The manipulation comprised steps of: providing a biomolecule combined with at least one ssDNA combined with a first protein through an affinity binding tag; adding a second ssDNA conjugated with a second protein with a concentration greater than that of the first protein; and replacing the first protein on the ssDNA with the second protein through chemical competitive principle. The invention may comprise the steps with proper design of biotinylated DNA probes, the functionalized ssDNA nanotemplates can be recovered to its unbound state through a thermodynamic principle.
    Type: Grant
    Filed: April 2, 2012
    Date of Patent: October 22, 2013
    Assignee: National Applied Research Laboratories
    Inventors: Ming-Yu Liu, Yuh-Shyong Yang, Yen-Pei Lu, Chih-Heng Lin
  • Patent number: 8545697
    Abstract: A device for the treatment of waste material in a waste water collection system includes an inner core and an outer portion partially surrounding the inner core such that at least one surface of the inner core is exposed. The inner core has a greater water solubility than the outer portion.
    Type: Grant
    Filed: August 9, 2012
    Date of Patent: October 1, 2013
    Assignee: United Laboratories, Inc.
    Inventors: Vincent J. Thorgersen, John H. Bacon, Eric D. Frazier
  • Patent number: 8535924
    Abstract: A granule with an allergenic component has reduced dust by including antifoam added during the production of the granule. The antifoam may be dispersed throughout the granule or added to one of the components of the granule. The granule with antifoam produces at least 30% less dust than a comparable granule produced according to a process in which no antifoam is added.
    Type: Grant
    Filed: November 3, 2011
    Date of Patent: September 17, 2013
    Assignee: Danisco US Inc.
    Inventors: Douglas A. Dale, Thomas S. Green
  • Patent number: 8377669
    Abstract: The invention is directed to methods and devices for reducing interference from leukocytes in an analyte immunoassay, and in particular in non-competitive immunoassays. In one embodiment, the invention is to a method comprising the steps of (a) amending a biological sample such as a whole blood sample with sacrificial beads; and (b) performing a non-competitive immunoassay on the amended sample to determine the concentration of said analyte in said sample. Preferably, the sample is amended with IgG-coated sacrificial beads.
    Type: Grant
    Filed: November 17, 2009
    Date of Patent: February 19, 2013
    Assignee: Abbott Point of Care Inc.
    Inventors: John Lewis Emerson Campbell, Adam Roger Moss, Graham Davis
  • Patent number: 8377664
    Abstract: The present invention provides a method for producing a useful substance by supplying, to a fixed-bed reactor packed with an immobilized enzyme, a liquid mixture containing two liquid phases, in which the two liquid phases are allowed to flow in an identical, parallel direction, which method employs a fixed-bed reactor equipped with an insertion unit or tubes, so as to form a plurality of lumens in the fixed-bed reactor, each lumen having a cross section of a circular shape with a diameter of 100 mm or less or having a polygonal shape with a diagonal line of 100 mm or less, wherein the lumens are packed with an immobilized enzyme and the liquid mixture is supplied. In a reaction performed by passing a reaction mixture exhibiting two liquid phases through a fixed-bed reactor equipped with an immobilized enzyme, overall flow of the reaction liquid is made uniform.
    Type: Grant
    Filed: October 4, 2006
    Date of Patent: February 19, 2013
    Assignee: Kao Corporation
    Inventors: Jun Saito, Yoshitaka Senda, Toshiteru Komatsu
  • Patent number: 8329422
    Abstract: The methods and reagents described in this invention are used to analyze circulating tumor cells, clusters, fragments, and debris. Analysis is performed with a number of platforms, including flow cytometry and the CELLSPOTTER® fluorescent microscopy imaging system. Analyzing damaged cells has shown to be important. However, there are two sources of damage: in vivo and in vitro. Damage in vivo occurs by apoptosis, necrosis, or immune response. Damage in vitro occurs during sample acquisition, handling, transport, processing, or analysis. It is therefore desirable to confine, reduce, eliminate, or at least qualify in vitro damage to prevent it from interfering in analysis. Described herein are methods to diagnose, monitor, and screen disease based on circulating rare cells, including malignancy as determined by CTC, clusters, fragments, and debris. Also provided are kits for assaying biological specimens using these methods.
    Type: Grant
    Filed: November 2, 2010
    Date of Patent: December 11, 2012
    Assignee: Veridex LLC
    Inventors: Galla Chandra Rao, Christopher Larson, Madeline Repollet, Herman Rutner, Leon W. M. M. Terstappen, Shawn Mark O'Hara, Steven Gross
  • Publication number: 20120301930
    Abstract: The present invention relates to bio-engineered multi-enzyme complexes having synergistic enzyme activity comprising xylanases and optionally further comprising additional carbohydrate active enzymes. Such complexes are advantageous for degrading recalcitrant cellulosic biomass.
    Type: Application
    Filed: February 15, 2011
    Publication date: November 29, 2012
    Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.
    Inventors: Edward A. Bayer, Yoav Barak, Sarah Morais
  • Patent number: 8304220
    Abstract: Bone cages are disclosed including devices for biocompatible implantation. The structures of bone are useful for providing living cells and tissues as well as biologically active molecules to subjects.
    Type: Grant
    Filed: October 15, 2007
    Date of Patent: November 6, 2012
    Assignee: The Invention Science Fund I, LLC
    Inventors: Ed Harlow, Edward K. Y. Jung, Robert Langer, Eric C. Leuthardt, Lowell L. Wood, Jr.
  • Patent number: 8298425
    Abstract: A methodology, and related systems and structures for accomplishing the methodology, of biological remediation of hazardous or undesirable organic matter, wherein a plurality of carrier members are disposed in a localized retaining member, the carrier members releasing or exposing microorganisms to the undesirable organic matter on a staggered basis over an extended period of time, the microorganisms being capable of biologically remediating the undesirable organic matter by utilizing the organic matter as a food source, thereby converting it into environmentally safe bi-products. Carrier members containing nutrients necessary for the survival of the microorganisms and having release times corresponding to the release times of the microorganisms are also provided to insure that the microorganisms remain viable upon release.
    Type: Grant
    Filed: April 30, 2010
    Date of Patent: October 30, 2012
    Inventor: Mark D. Shaw
  • Patent number: 8252560
    Abstract: The present invention relates to a process for producing a fatty acid, which includes feeding a liquid mixture formed of two liquid phases that flow in a co-current manner into a fixed bed-type reaction column packed with immobilized lipase, and performing a hydrolysis reaction when the two liquid phases are in contact with the immobilized lipase in the fixed-bed reaction column; wherein the fixed-bed reaction column comprises partition plates so as to comprise a plurality of tube- shaped structures, wherein each tube-shaped structure has a cross -section which is rectangular, circular, oval, or polygonal in shape with at least a part being unclosed and the cross-section has a representative length of 100 mm or less.
    Type: Grant
    Filed: December 14, 2007
    Date of Patent: August 28, 2012
    Assignee: Kao Corporation
    Inventors: Jun Saito, Yoshitaka Senda, Keigo Hanaki, Toshiteru Komatsu
  • Patent number: 8252570
    Abstract: Bone cages are disclosed including devices for biocompatible implantation. The structures of bone are useful for providing living cells and tissues as well as biologically active molecules to subjects.
    Type: Grant
    Filed: September 12, 2007
    Date of Patent: August 28, 2012
    Assignee: The Invention Science Fund I, LLC
    Inventors: Ed Harlow, Edward K. Y. Jung, Robert Langer, Eric C. Leuthardt, Lowell L. Wood, Jr.
  • Patent number: 8236518
    Abstract: Disclosed are antibodies that selectively bind to blood coagulation factor FVIII, and highly sensitive immunological assays comprising these antibodies. Preferred assays can detect FVIII at about 3500-fold below the normal physiological levels, and have a wide array of applications including accurate monitoring of FVIII concentration in pharmaceutical products for treatment of blood coagulation disorders, and determination of FVIII levels in plasma of human patients, including those with blood coagulation disorders such as hemophilia.
    Type: Grant
    Filed: July 13, 2006
    Date of Patent: August 7, 2012
    Assignee: The University of Vermont and State Agriculture College
    Inventors: Behnaz Parhami-Seren, Kenneth G. Mann, David N. Fass
  • Publication number: 20120177913
    Abstract: Provided are a novel use of a lipolytic enzyme for forming anti-fingerprint coating, a method of forming anti-fingerprint coating including treating a substrate with a composition comprising the lipolytic enzyme, a substrate including the anti-fingerprint coating formed by the same method, and a product including the same. The anti-fingerprint coating can reduce contamination of display devices, appearances of electronic devices or building materials by fingerpris.
    Type: Application
    Filed: September 17, 2010
    Publication date: July 12, 2012
    Inventors: Eun Jeong Lee, Young Jun Hong, Hveon Choi, Taek Ho Yang
  • Publication number: 20120040395
    Abstract: Enzyme catalyst immobilized on porous flouropolymer supports. Catalytically active enzymes can be bound to a variety of fluoropolymer supports for use as a reaction catalyst. Moreover, consistently high rate of conversion during catalyst re-use over time is achieved. Furthermore, inactive enzyme catalyst can be stripped from the support and fresh enzyme can be bound to the support to achieve the original conversion rate. The immobilization of catalytic enzymes on porous fluoropolymers is a viable and novel technology for the preparation of advantaged catalysts.
    Type: Application
    Filed: August 12, 2010
    Publication date: February 16, 2012
    Applicant: EASTMAN CHEMICAL COMPANY
    Inventor: Stephanie Kay Clendennen
  • Patent number: 8114647
    Abstract: Bone cages are disclosed including devices for biocompatible implantation. The structures of bone are useful for providing living cells and tissues as well as biologically active molecules to subjects.
    Type: Grant
    Filed: October 18, 2007
    Date of Patent: February 14, 2012
    Assignee: The Invention Science Fund I, LLC
    Inventors: Ed Harlow, Roderick A. Hyde, Edward K. Y. Jung, Robert Langer, Eric C. Leuthardt, Lowell L. Wood, Jr.
  • Patent number: 8076113
    Abstract: A granule with an allergenic component has reduced dust by including antifoam added during the production of the granule. The antifoam may be dispersed throughout the granule or added to one of the components of the granule. The granule with antifoam produces at least 30% less dust than a comparable granule produced according to a process in which no antifoam is added.
    Type: Grant
    Filed: April 1, 2002
    Date of Patent: December 13, 2011
    Assignee: Danisco US Inc.
    Inventors: Douglas A. Dale, Thomas S. Green
  • Publication number: 20110262963
    Abstract: Disclosed herein is pyrroline-carboxy-lysine (PCL), a pyrrolysine analogue, which is a natural, biosynthetically generated amino acid, and methods for biosynthetically generating PCL. Also disclosed herein are proteins, polypeptides and peptides that have PCL incorporated therein and methods for incorporating PCL into such proteins, polypeptides and peptides. Also disclosed herein is the site-specific derivatization of proteins, polypeptides and peptides having PCL or pyrrolysine incorporated therein. Also disclosed herein is the crosslinking of proteins, polypeptides and peptides having PCL or pyrrolysine incorporated therein.
    Type: Application
    Filed: October 23, 2009
    Publication date: October 27, 2011
    Inventors: Bernhard Geierstanger, Weijia Ou, Susan E. Cellitti, Tetsuo Uno, Tiffany Crossgrove, Hsien-Po Chiu, Jan Grunewald, Xueshi Hao
  • Patent number: 7993890
    Abstract: The present invention relates to a process for enzymatic hydrolysis of arabinoxylan, and an enzyme composition suitable for use in such a process.
    Type: Grant
    Filed: April 25, 2006
    Date of Patent: August 9, 2011
    Assignee: Novozymes A/S
    Inventors: Hanne Risbjerg Soerensen, Sven Pedersen, Anders Viksoe-Nielsen, Christel Thea Joergensen, Lars Hylling Christensen, Christian Isak Joergensen, Carsten Hoerslev Hansen, Lene Venke Kofod
  • Publication number: 20110059373
    Abstract: The present invention relates to a system for production of ATP. This system is comprised of a support and one or more enzymes coupled to that support which are capable of collectively producing ATP from glucose or fructose metabolism. The present invention is additionally directed to a device, which includes the system, and to a method for carrying out a reaction involving the conversion of ATP to ADP using the system.
    Type: Application
    Filed: October 16, 2008
    Publication date: March 10, 2011
    Applicant: Cornell Research Foundation, Inc.
    Inventor: Alexander Travis
  • Patent number: 7867740
    Abstract: Nucleic acids encoding Thermotoga maritima mannitol dehydrogenase and the Thermotoga maritima mannitol dehydrogenase polypeptide are disclosed. Further provided are an electrochemical bioreactor system and a bioreactor electrode that can be used to convert glucose or fructose to mannitol.
    Type: Grant
    Filed: August 10, 2007
    Date of Patent: January 11, 2011
    Assignee: Board of Trustees of Michigan State University
    Inventors: Claire Vieille, Seung Hoon Song, J. Gregory Zeikus
  • Patent number: 7855062
    Abstract: Bone cages are disclosed including devices for biocompatible implantation. The structures of bone are useful for providing living cells and tissues as well as biologically active molecules to subjects.
    Type: Grant
    Filed: December 14, 2005
    Date of Patent: December 21, 2010
    Assignee: The Invention Science Fund I, LLC
    Inventors: Ed Harlow, Edward K. Y. Jung, Robert Langer, Eric C. Leuthardt, Lowell L. Wood, Jr.
  • Patent number: 7846748
    Abstract: The present invention provides methods of quantifying the amount or concentration of one or more peptides and/or proteins in one or more samples using differentially isotopically-labeled peptides and/or proteins. The invention also provides methods of identifying one or more peptides and/or proteins in one or more samples.
    Type: Grant
    Filed: April 11, 2003
    Date of Patent: December 7, 2010
    Assignee: The University of North Carolina at Chapel Hill
    Inventor: Christoph H. Borchers
  • Patent number: 7811796
    Abstract: A multilayer enzyme immobilization process is provided comprising adsorbing a polyethyleneimine (PEI) solution in a fibrous matrix, and adding an enzyme to the fibrous matrix, which comprises a plurality of fibrils. The process further comprises forming at least two layers of PEI-enzyme aggregates on the fibrils, and cross-linking the multilayer PEI-enzyme aggregates. The process can further comprise washing the fibrils containing the cross-linked PEI-enzyme aggregates with distilled water and acetic acid buffer subsequent to cross-linking. However, the PEI-containing matrix is not washed prior to the addition of enzyme. The enzyme can be ?-galactosidase and the fibrous matrix can be cotton cloth. The multilayer immobilized enzyme can be employed in a biocatalyst reactor for production of galacto-oligosaccharides from lactose and the hydrolysis of lactose to glucose and galactose.
    Type: Grant
    Filed: December 4, 2006
    Date of Patent: October 12, 2010
    Assignee: The Ohio State University Research Foundation
    Inventors: Shang-Tian Yang, Nedim Albayrak
  • Patent number: 7691617
    Abstract: The present invention relates to an acid-stable alpha amylase (asAA) derived from a strain of Aspergillus kawachi, which has granular starch hydrolyzing (GSH) activity, the heterologous expression of the asAA having GSH activity in filamentous fungal host cells and enzyme compositions including the same which optionally include glucoamylase.
    Type: Grant
    Filed: January 30, 2008
    Date of Patent: April 6, 2010
    Assignee: Danisco US Inc.
    Inventors: Nigel Dunn-Coleman, Susan M. Fiske, Suzanne E. Lantz, Paulien Neefe-Kruithof, Michael J. Pepsin, Jayarama K. Shetty
  • Publication number: 20100021986
    Abstract: Provided is a cell chip for exposing to a portion of each of cells microdroplets dispensed with an inkjet printer or the like in a screening test of the effect of a substance on cells by using an apparatus for introducing the substance into cells including: a cell-immobilizing support including a substrate having through-holes penetrating from one side to the other side of the substrate and including cells immobilized in the through-holes so as to block up the through-holes; a liquid phase region present in contact with one side of the support and including a medium of the cells; and a mechanism present so as to face the other side of the support and imparting a microdroplet to a portion of each of the cells exposed in the openings on the other side.
    Type: Application
    Filed: January 17, 2008
    Publication date: January 28, 2010
    Applicant: CANON KABUSHIKI KAISHA
    Inventors: Kouichi Kato, Atsushi Takahashi
  • Patent number: 7585659
    Abstract: Described herein is a novel three domain gene from Schizochytrium, denoted carotene synthase, that encodes a protein with three different enzymatic activities: phytoene dehydrogenase (PD), phytoene synthase (PS), and lycopene cyclase (LC). Also described is the isolated gene encoding the carotene synthase, homologues thereof, the enzyme encoded by such gene, biologically active portions and homologues thereof, recombinant nucleic acid molecules, microorganisms and plants that have been genetically modified to increase or decrease the action of such gene, and methods of producing carotenoids and derivatives thereof or methods of producing microorganisms and lipid products lacking pigmentation using the knowledge of the carotene synthase described herein.
    Type: Grant
    Filed: November 8, 2006
    Date of Patent: September 8, 2009
    Assignee: Martek Biosciences Corporation
    Inventors: Craig A. Weaver, James G. Metz, Jerry M. Kuner, Frank H. Overton, Jr.
  • Patent number: 7582451
    Abstract: Described herein is a novel three domain gene from Schizochytrium, denoted carotene synthase, that encodes a protein with three different enzymatic activities: phytoene dehydrogenase (PD), phytoene synthase (PS), and lycopene cyclase (LC). Also described is the isolated gene encoding the carotene synthase, homologues thereof, the enzyme encoded by such gene, biologically active portions and homologues thereof, recombinant nucleic acid molecules, microorganisms and plants that have been genetically modified to increase or decrease the action of such gene, and methods of producing carotenoids and derivatives thereof or methods of producing microorganisms and lipid products lacking pigmentation using the knowledge of the carotene synthase described herein.
    Type: Grant
    Filed: November 8, 2006
    Date of Patent: September 1, 2009
    Assignee: Martek Biosciences Corporation
    Inventors: Craig A. Weaver, James G. Metz, Jerry M. Kuner, Frank H. Overton, Jr.
  • Patent number: 7544438
    Abstract: An object of the present invention is to provide a highly functional mediator capable of smoothly proceeding electron transfer between an electrode and a biocatalyst based on the premise that the mediator excels in safety and cost and to provide an electrode and a cell with sufficient amount of such a mediator molecule immobilized efficiently and strongly. The present invention provides a mediator mediating electron transfer between an enzyme and an electrode and containing a quinone molecule derivative, in particular, a mediator containing a VK3 derivative. In addition, the present invention provides an electrode and a biofuel cell to which those mediators are applied.
    Type: Grant
    Filed: March 23, 2004
    Date of Patent: June 9, 2009
    Assignee: Daiichi Pure Chemicals Co., Ltd
    Inventors: Matsuhiko Nishizawa, Tomokazu Matsue, Jun-ichi Kosuge, Noboru Fukasaku
  • Publication number: 20080274527
    Abstract: The present invention relates to a process for enzymatic hydrolysis of arabinoxylan, and an enzyme composition suitable for use in such a process.
    Type: Application
    Filed: April 25, 2006
    Publication date: November 6, 2008
    Applicant: Novozymes A/S
    Inventors: Hanne Risbjerg Soerensen, Sven Pedersen, Anders Viksoe-Nielsen, Christel Thea Joergensen, Lars Hylling Christensen, Christian Isak Joergensen, Carsten Hoerslev Hansen, Lene Venke Kofod
  • Patent number: 7427497
    Abstract: Disclosed herein is a microfluidics device that can be used to prepare natural products and their analogs. The device comprises the enzymes of a biosynthetic pathway immobilized thereon and a means for sequentially directing a starting material and each ensuing reaction product to the enzymes of the biosynthetic pathway in the order corresponding to the steps of the biosynthetic pathway. The device can thus be used to prepare the natural product using the natural starting material of the biosynthetic pathway or analogs of the natural product using an unnatural starting material. Alternatively, artificial pathways can be created by immobilizing an appropriate selection of enzymes on the device in an order whereby each subsequent enzyme can catalyze a reaction with the product of the prior enzyme. Novel chemical entities can be prepared from these artificial pathways.
    Type: Grant
    Filed: November 1, 2002
    Date of Patent: September 23, 2008
    Assignees: Rensselaer Polytechnic Institute, Darpa, The Regents of the University of California
    Inventors: Jonathan S. Dordick, Aravind Srinivasan, Jungbae Kim, David H. Sherman, Douglas S. Clark
  • Patent number: 7402381
    Abstract: Aspects of the invention can provide a method capable of easily realizing immobilization with the optimum density derived from a concentration control and without phase separation in coadsorption of a number of molecules. The immobilization method can include the step of dissolving a plurality of molecules to be immobilized to a solid phase substrate with a solvent to obtain a solution of the plurality of molecules, and the step of incubating the solution and the solid phase substrate in touch therewith. Each of the molecules can include a solid phase substrate joint portion having a jointing property to the solid phase substrate, a functional portion having a specific function, and a linker portion positioned between the solid phase substrate joint portion and the functional portion.
    Type: Grant
    Filed: August 27, 2004
    Date of Patent: July 22, 2008
    Assignee: Seiko Epson Corporation
    Inventors: Hiroshi Takiguchi, Hitoshi Fukushima
  • Patent number: 7364884
    Abstract: A process for preparing a hydroxylation catalyst by i) embedding a cystochrome P450 monooxygenase in a sol-gel matrix, ii) embedding an enzymatic NADPH-regenerating system in a sol-gel matrix, and combining the two components i) and ii) unless they were already mixed together before the embedding.
    Type: Grant
    Filed: May 5, 2004
    Date of Patent: April 29, 2008
    Assignee: BASF Aktiengesellschaft
    Inventors: Bernhard Hauer, Tilo Habicher, Rolf Schmid, Steffen Christian Maurer, Vlada Beniaminovna Urlacher, Holger Schulze, Norbert Huber, Till T. Bachmann
  • Patent number: 7354752
    Abstract: The present invention relates to an acid-stable alpha amylase (asAA) derived from a strain of Aspergillus kawachi, which has granular starch hydrolyzing (GSH) activity, the heterologous expression of the asAA having GSH activity in filamentous fungal host cells and enzyme compositions including the same which optionally include glucoamylase.
    Type: Grant
    Filed: May 24, 2005
    Date of Patent: April 8, 2008
    Assignee: Genencor International, Inc.
    Inventors: Nigel Dunn-Coleman, Susan M. Fiske, Suzanne E. Lantz, Paulien Neefe-Kruithof, Michael J. Pepsin, Jayarama K. Shetty
  • Patent number: 7348169
    Abstract: A bioactive catalytic material is disclosed for providing protection from chemical exposure. The material is composed of enzymes immobilized within polyelectrolyte multilayers and a polymerizable end-capping layer to render stability to enzymes. Also disclosed is the related method for making a bioactive catalytic material and their deposition on substrates of varying size, shape and flexibility for providing active protection from chemical exposure.
    Type: Grant
    Filed: May 4, 2006
    Date of Patent: March 25, 2008
    Assignee: The United States of America as represented by the Secretary of the Navy
    Inventors: Alok Singh, Yongwoo Lee, Evan Stanish, Eddie Chang, Walter J. Dressick