Preparing Granular- Or Free-flowing Enzyme Composition Patents (Class 435/187)
  • Patent number: 10723980
    Abstract: Described herein are cleaning compositions comprising fermented fruit solutions and builders, methods for making the same, and methods for using the same. The fermented fruit solutions can contain fruit, sugar and water. The builder can be selected from the group consisting of a non-phosphate builder, such as sodium citrate and sodium bicarbonate, boric acid and mixtures thereof. The cleaning compositions can be used to clean articles, launder articles, clean stains from articles, and clean surfaces.
    Type: Grant
    Filed: June 5, 2018
    Date of Patent: July 28, 2020
    Assignee: EQUATOR GLOBAL LIMITED
    Inventors: Peter Nelson Wainman, Sirilak Narongtanupone, Sungworn Sangsri
  • Patent number: 10717115
    Abstract: The present disclosure relates to compositions and methods for cleaning medical and dental instruments. The disclosed compositions are preferably non-foaming or generate low foam to allow visual inspection of the cleaning process as well as safe handling of the instruments. The disclosed compositions preferably employ select proteases, a carbonate and a nonionic surfactant.
    Type: Grant
    Filed: August 4, 2017
    Date of Patent: July 21, 2020
    Assignee: Ecolab USA Inc.
    Inventors: Nathan R. Ortmann, Katherine Vetter, Thomas R. Mohs, Anthony W. Erickson
  • Patent number: 10538720
    Abstract: A composition including a plurality of particles. The particles include from about 40% to about 99% by weight of said particles of a carrier and from about 0.0001% to about 5% by weight of an enzyme. Each of the particles has a mass between about 1 mg to about 5000 mg.
    Type: Grant
    Filed: March 8, 2016
    Date of Patent: January 21, 2020
    Assignee: The Procter & Gamble Company
    Inventors: Montserrat Guadalupe Vasquez Valdivieso, Neil Joseph Lant
  • Patent number: 10131863
    Abstract: The present invention concerns a detergent and a pharmaceutical composition comprising a deoxyribonuclease (DNase), wherein the DNase is obtained from a fungal source. It further concerns a laundering method and the use of DNase. The present invention further relates to polypeptides having DNase activity, nucleotides encoding the polypeptide, as well as methods of producing the polypeptides.
    Type: Grant
    Filed: April 10, 2015
    Date of Patent: November 20, 2018
    Assignee: NOVOZYMES A/S
    Inventors: Klaus Gori, Marie Allesen-Holm, Lillian Eva Tang Baltsen, Allan Noergaard, Jan Lehmbeck
  • Patent number: 10087114
    Abstract: Biocatalytic composition to be used in the agricultural, zootechnical and environmental recovery fields, to transform substrates which is reacted with, to non-polluting substances, including a component with coenzymatic activity including Vitamin A, Vitamin D3, Vitamin E, Propylgallate, Raw fats, Raw proteins; a component with enzymatic activity including Bacillus licheniformis, Bacillus subtilis, Bacillus thuringiensis, Bacillus SPP, Aspergillus Oryzae, Aspergillus Niger, Lactobacillus bifidus, Lactobacillus acidophilus, Amylase, Protease, Lipase, Cellulase, Gumase; and a component including substances regulating pH, including Humic acids, Fulvic acids, Crenic acids, Apocrenic acids, Vitamin A, Vitamin D3, Vitamin PP, Arthrospira maxima, among others.
    Type: Grant
    Filed: February 11, 2015
    Date of Patent: October 2, 2018
    Assignee: BIOMA SA
    Inventor: Elio Fabio Bortoli
  • Patent number: 10011808
    Abstract: Stabilized use solutions of low phosphorus, alkali metal carbonate detergents employing enzymes for cleaning compositions are disclosed. In particular, the present invention is a composition for, and method of, removing soils, preventing redeposition of protein soils and reducing foam, using stabilized enzyme cleaning compositions, namely use solutions of the same.
    Type: Grant
    Filed: November 10, 2014
    Date of Patent: July 3, 2018
    Assignee: Ecolab USA Inc.
    Inventors: Wendy Chan, Jennifer Stokes, Lyndal Jensen, Carter M. Silvernail, Terrence P. Everson, Graig Legatt, Nathan Richard Ortmann, Devon Beau Hammel
  • Patent number: 9890429
    Abstract: The present invention relates to compositions, kits, and methods for detecting, characterizing, preventing, and treating cancer (e.g., hematological malignancies in humans). A variety of biomarker chromosomal number alterations (CNAs) and biomarkers corresponding thereto, are provided, wherein alterations in the copy number of one or more of the biomarker CNAs and/or alterations in the amount, structure, and/or activity of one or more of the biomarkers comprised within the CNAs is associated with cancer status.
    Type: Grant
    Filed: February 28, 2013
    Date of Patent: February 13, 2018
    Assignees: Dana-Farber Cancer Institute, Inc., The Broad Institute, Inc.
    Inventors: Margaret A. Shipp, Stefano Monti, Bjoern Chapuy, Scott J. Rodig, Todd R. Golub
  • Patent number: 9598661
    Abstract: Described herein are cleaning compositions comprising fermented fruit solutions and builders, methods for making the same, and methods for using the same. The fermented fruit solutions can contain fruit, sugar and water. The builder can be selected from the group consisting of a non-phosphate builder, such as sodium citrate and sodium bicarbonate, boric acid and mixtures thereof. The cleaning compositions can be used to clean articles, launder articles, clean stains from articles, and clean surfaces.
    Type: Grant
    Filed: August 26, 2016
    Date of Patent: March 21, 2017
    Assignee: EQUATOR GLOBAL LIMITED
    Inventors: Peter Nelson Wainman, Sirilak Narongtanupone, Sungworn Sangsri
  • Patent number: 9598662
    Abstract: Described herein are natural cleaning compositions comprising fermented fruit solutions, methods for making the same, and methods for using the same. The fermented fruit solutions can contain fruit, sugar and water. Further described herein are methods of making fermented fruit solutions for use with cleaning compositions. The fermented fruit solutions described herein can be used in cleaning compositions including laundry detergents, stain removers, fabric softeners, floor cleaners, bathroom cleaners, dishwashing products, kitchen cleaners, liquid soap, multi-purpose cleaners and the like.
    Type: Grant
    Filed: October 11, 2016
    Date of Patent: March 21, 2017
    Assignee: EQUATOR GLOBAL LIMITED
    Inventors: Sirilak Narongtanupone, Peter Nelson Wainman, Areerat Lertamornchaikul
  • Patent number: 9493729
    Abstract: Described herein are natural cleaning compositions comprising fermented fruit solutions, methods for making the same, and methods for using the same. The fermented fruit solutions can contain fruit, sugar and water. Further described herein are methods of making fermented fruit solutions for use with cleaning compositions. The fermented fruit solutions described herein can be used in cleaning compositions including laundry detergents, stain removers, fabric softeners, floor cleaners, bathroom cleaners, dishwashing products, kitchen cleaners, liquid soap, multi-purpose cleaners and the like.
    Type: Grant
    Filed: September 20, 2013
    Date of Patent: November 15, 2016
    Assignee: EQUATOR GLOBAL LIMITED
    Inventors: Sirilak Narongtanupone, Peter Nelson Wainman, Areerat Lertamornchaikul
  • Patent number: 9434912
    Abstract: Described herein are softening compositions comprising fermented fruit solutions and softening agents, methods for making the same, and methods for using the same. The fermented fruit solutions can contain fruit, sugar and water. The softening agent can be selected from the group consisting of soy lecithin, oleic acid, glycerol, and mixtures thereof. The softening compositions can be used to soften article(s) (e.g., using as a fabric softener).
    Type: Grant
    Filed: May 20, 2014
    Date of Patent: September 6, 2016
    Assignee: EQUATOR GLOBAL LIMITED
    Inventors: Peter Nelson Wainman, Sirilak Narongtanupone, Rosalin Dokphut, Areerat Lertamornchaikul, Sungworn Sangsri
  • Patent number: 9434913
    Abstract: Described herein are laundry detergent compositions comprising fermented fruit solutions and natural based surfactants, methods for making the same, and methods for using the same. The fermented fruit solutions can contain fruit, sugar and water. The natural based surfactant can be selected from the group consisting of sodium lauryl sulfate, cocamidopropyl betaine, alkyl polyglycoside, and mixtures thereof. The laundry detergent compositions can be used to clean articles, launder articles, and clean stains from articles.
    Type: Grant
    Filed: October 2, 2013
    Date of Patent: September 6, 2016
    Assignee: EQUATOR GLOBAL LIMITED
    Inventors: Peter Nelson Wainman, Sirilak Narongtanupone, Sungworn Sangsri
  • Patent number: 9434914
    Abstract: Described herein are cleaning compositions comprising fermented fruit solutions and builders, methods for making the same, and methods for using the same. The fermented fruit solutions can contain fruit, sugar and water. The builder can be selected from the group consisting of a non-phosphate builder, such as sodium citrate and sodium bicarbonate, boric acid and mixtures thereof. The cleaning compositions can be used to clean articles, launder articles, clean stains from articles, and clean surfaces.
    Type: Grant
    Filed: October 16, 2013
    Date of Patent: September 6, 2016
    Assignee: EQUATOR GLOBAL LIMITED
    Inventors: Peter Nelson Wainman, Sirilak Narongtanupone, Sungworn Sangsri
  • Patent number: 9139458
    Abstract: Provided herein are compositions and methods that can remove, metabolize, or degrade a hydrocarbon in an area that is contaminated by hydrocarbons. Methods for bioremediation of an area such as an area of land, a body of water, or a shoreline that are contaminated by a hydrocarbon, such as from a crude oil spill are also described. The compositions and methods described herein can be used on natural flora and fauna as well as manmade materials that are contaminated by a hydrocarbon.
    Type: Grant
    Filed: July 23, 2013
    Date of Patent: September 22, 2015
    Inventor: Janet Angel
  • Patent number: 9107433
    Abstract: The stability of enzymes in a powder detergent can be very significantly improved by the combination of four measures: Addition of reducing agent/peroxide decomposing catalyst/antioxidant to the core or the coating; Addition of a multivalent cation to the core; Addition of an acidic buffer to the core or to the coating; Applying a salt coating onto the core.
    Type: Grant
    Filed: April 15, 2011
    Date of Patent: August 18, 2015
    Assignee: Novozymes A/S
    Inventors: Flemming Borup, Morten Mohr Hansen, Poul Bach, Ole Simonsen
  • Patent number: 8884766
    Abstract: An analysis system includes: a creatinine information acquiring section to input creatinine measurement data that indicate a measurement quantity of creatinine in a urine sample; a material ingredient information acquiring section to input material ingredient data that indicate a measured quantity of a material ingredient in the urine sample; and a correcting section to correct the measured quantity of the material ingredient indicated as the material ingredient data, by use of the measured quantity of creatinine indicated as the creatinine measurement data.
    Type: Grant
    Filed: April 25, 2012
    Date of Patent: November 11, 2014
    Assignee: ARKRAY, Inc.
    Inventor: Shinya Nakajima
  • Patent number: 8846361
    Abstract: The present invention relates to solid enzyme, in particular phytase, compositions stabilized with a lactic acid source such as Corn Steep Liquor (CSL), and methods of producing the same. Preferred compositions additionally comprise starch and disaccharides such as lactose or trehalose.
    Type: Grant
    Filed: March 13, 2009
    Date of Patent: September 30, 2014
    Assignee: Novozymes A/S
    Inventors: Lotte Rugholm Henriksen, Erik Marcussen
  • Publication number: 20140220655
    Abstract: Articles and methods for forming nanostructures having unique and/or predetermined shapes are provided. The methods and articles may involve the use of nucleic acid containers as structural molds. For instance, a pre-designed nucleic acid container including a cavity may be used to control the shape-specific growth of nanoparticles. Growth of the nanoparticles within the cavities may be confined by the specific shape of the nucleic acid container. In some embodiments, the resulting nucleic acid-nanoparticle structures can be used to control the orientation and numbers of surface ligands on the surface of nanoparticles. The addressability of the surface ligands can be used to form higher ordered assemblies of the structures.
    Type: Application
    Filed: June 29, 2012
    Publication date: August 7, 2014
    Applicant: President and Fellows of Harvard College
    Inventors: Wei Sun, Peng YI
  • Publication number: 20140206035
    Abstract: Provided are isolated polypeptides having glucoamylase activity, catalytic domains, and polynucleotides encoding the polypeptides, catalytic domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains.
    Type: Application
    Filed: August 24, 2012
    Publication date: July 24, 2014
    Inventors: Tianqi Sun, Ming Li
  • Patent number: 8759057
    Abstract: A process for purifying laccase from an ectomycorrhizal fruiting body is disclosed. The process includes steps of homogenization, sonication, centrifugation, filtration, affinity chromatography, ion exchange chromatography, and gel filtration. Purified laccase can also be separated into isomers.
    Type: Grant
    Filed: April 1, 2013
    Date of Patent: June 24, 2014
    Assignee: The United States of America as Represented by the Administrator of the National Aeronautics & Space Administration (NASA)
    Inventors: Kenneth W. Cullings, Julia C. DeSimone, Chad D. Paavola
  • Publication number: 20140057324
    Abstract: Variants of Bacillus sp. no. 707 alpha-amylase are provided that are produced more efficiently and thus more economically. Higher fermentation yields are achieved through introducing amino acid variations that promote solubility of the variant in a fermentation broth. Increased solubility allows more enzyme to remain in solution after expression in a host cell. This in turn increases the efficiency with which the expressed variant enzyme can be recovered from the fermentation broth.
    Type: Application
    Filed: August 23, 2013
    Publication date: February 27, 2014
    Applicant: Danisco US Inc.
    Inventors: Wolfgang AEHLE, Neelam S. AMIN
  • Publication number: 20140017715
    Abstract: The invention relates to a variant of a parent Termamyl-like ?-amylase, which exhibits an alteration in at least one of the following properties relative to said parent ?-amylase: i) improved pH stability at a pH from 8 to 10.5; and/or ii) improved Ca2+ stability at pH 8 to 10.5, and/or iii) increased specific activity at temperatures from 10 to 60° C.
    Type: Application
    Filed: March 5, 2013
    Publication date: January 16, 2014
    Applicant: NOVOZYMES A/S
    Inventors: Torben Vedel Borchert, Allan Svendsen, Carsten Andersen, Bjarne Nielsen, Torben Lauesgaard Nissen, Soren Kjaerulff
  • Publication number: 20130337531
    Abstract: Methods are provided for recovery and formulation of insoluble enzymes from a microbial fermentation broth, without removal of microbial cells or cell debris. Granular and liquid formulations comprising insoluble enzymes are also provided.
    Type: Application
    Filed: June 10, 2013
    Publication date: December 19, 2013
    Inventors: Michael BODO, Robert I. CHRISTENSEN, Rajdeep S. DHALIWAL, Meng H. HENG
  • Patent number: 8603339
    Abstract: Excess sludge production in a bioreactor of a wastewater treatment plant in which excess sludge is being produced is reduced by adding an activated sludge material having a chitinase specific activity of at least 150 Units/g-MLSS and a pectinase specific activity of at least 120 Units/g-MLSS to the bioreactor. After the addition of the activated sludge material, the bioreactor is controlled by adding the activated sludge material when any one of the chitinase activity, the pectinase activity, and the protease specific of the sludge in the bioreactor drops below the lower limits of 50 Units/L, 40 Units/L, and 0.3 Units/L, respectively.
    Type: Grant
    Filed: August 26, 2008
    Date of Patent: December 10, 2013
    Assignee: Diamond Engineering Co., Ltd.
    Inventors: Katsuhiko Maeda, Minoru Okamoto
  • Publication number: 20130288332
    Abstract: The invention provides small enzyme-containing granules having an inorganic salt core and an enzyme-containing layer coated over the core, and methods for producing such granules. The majority of the enzyme granules are less than 300 ?m in diameter. The granules are suitable for incorporation into compositions such as cleaning, textile processing, and animal feed compositions.
    Type: Application
    Filed: April 3, 2013
    Publication date: October 31, 2013
    Applicant: Danisco US Inc.
    Inventor: Danisco US Inc.
  • Publication number: 20130288314
    Abstract: Disclosed are a structure and a method capable of protecting from outside stimuli while containing in a liquid state a water-dispersible substance to be protected. The protective structure of the substance to be protected is a water-in-oil emulsion structure comprising an aqueous phase configuring a discontinuous phase containing the water-dispersible substance to be protected, an oil phase in which said aqueous phase is dispersed, and either vesicles formed with an amphiphilic substance which spontaneously forms vesicles or polycondensation polymer particles having hydroxyl groups.
    Type: Application
    Filed: June 17, 2013
    Publication date: October 31, 2013
    Inventors: Kazuo TAJIMA, Yoko IMAI
  • Publication number: 20130252850
    Abstract: The invention relates to a variant of a parent Termamyl-like alpha-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased.
    Type: Application
    Filed: May 20, 2013
    Publication date: September 26, 2013
    Applicant: NOVOZYMES A/S
    Inventors: Allan Svendsen, Soren Kjaerulff, Henrik Bisgaard-Frantzen, Carsten Andersen
  • Patent number: 8535924
    Abstract: A granule with an allergenic component has reduced dust by including antifoam added during the production of the granule. The antifoam may be dispersed throughout the granule or added to one of the components of the granule. The granule with antifoam produces at least 30% less dust than a comparable granule produced according to a process in which no antifoam is added.
    Type: Grant
    Filed: November 3, 2011
    Date of Patent: September 17, 2013
    Assignee: Danisco US Inc.
    Inventors: Douglas A. Dale, Thomas S. Green
  • Patent number: 8309334
    Abstract: The present invention provides highly impact-resistant, water-soluble or water dispersible, low-dust granules comprising an active ingredient and methods for obtaining the same.
    Type: Grant
    Filed: December 23, 2008
    Date of Patent: November 13, 2012
    Assignee: Danisco US Inc.
    Inventors: Nathaniel T. Becker, Mark S. Gebert, Isabelle Mazeaud
  • Patent number: 8273561
    Abstract: High pressure to treat aggregated interferons, particularly recombinant human interferon-?, to reduce the aggregate content of interferon material. Highly pure, soluble monomeric recombinant interferon-? is prepared in representative embodiments. Multiple strategies may be used in combination that make nonglycosylated IFN-? more amenable to high pressure treatment. It has been found that refolding yields of high pressure treatment can be significantly improved by use of a combination of strategies, including, or example a pre-treatment of the IFN-? that involves solubilizing and then precipitating the protein. This pre-treatment is particularly effective with respect to recombinant IFN-? inclusion bodies recovered from host cells such as E. coli cells. According to another strategy, refolding under high pressure is much more effective when the refolding reagent incorporating the IFN-? incorporates a zwitterionic surfactant and/or a cholate salt.
    Type: Grant
    Filed: October 6, 2008
    Date of Patent: September 25, 2012
    Assignee: Nuron Biotech, Inc.
    Inventors: Jeffrey L. Cleland, Stephen P. Eisenberg, Mary S. Rosendahl, Matthew B. Seefeldt
  • Patent number: 8076113
    Abstract: A granule with an allergenic component has reduced dust by including antifoam added during the production of the granule. The antifoam may be dispersed throughout the granule or added to one of the components of the granule. The granule with antifoam produces at least 30% less dust than a comparable granule produced according to a process in which no antifoam is added.
    Type: Grant
    Filed: April 1, 2002
    Date of Patent: December 13, 2011
    Assignee: Danisco US Inc.
    Inventors: Douglas A. Dale, Thomas S. Green
  • Publication number: 20110195477
    Abstract: The present invention discloses thermostable microcapsules comprising a semipermeable membrane, an aqueous core, one or more enzymes in the aqueous core, and a nucleic acid template for an enzyme-mediated reaction in the aqueous core. In one embodiment of the invention, the aqueous core contains one or more polymerases. Microcapsules of the invention can be used in amplification and sequencing reactions. In particular, the microcapsules of the present invention can be used for high-throughput sequencing.
    Type: Application
    Filed: August 6, 2009
    Publication date: August 11, 2011
    Applicant: Genome Corporation
    Inventor: Kevin Ulmer
  • Publication number: 20110189344
    Abstract: Methods are provided for recovery and formulation of insoluble enzymes from a microbial fermentation broth, without removal of microbial cells or cell debris. Granular and liquid formulations comprising insoluble enzymes are also provided.
    Type: Application
    Filed: June 9, 2009
    Publication date: August 4, 2011
    Inventors: Michael Bodo, Robert I. Christensen, Rajdeep S. Dhaliwal, Meng H. Heng
  • Patent number: 7960332
    Abstract: A coated granule including a core. The core includes a uniform mixture of a detergent enzyme having an alkaline pH activity optimum, and an acidic buffer component. The acidic buffer component has a pH of 1 to below 7 when measured as a 10% aqueous solution with a pKa in the range of 4 to 9.
    Type: Grant
    Filed: January 26, 2004
    Date of Patent: June 14, 2011
    Assignee: Novozymes A/S
    Inventors: Ole Simonsen, Erik Kjaer Markussen, Hanne Philbert Nielsen, Lone Aslaug Hansen
  • Patent number: 7923224
    Abstract: The present invention discloses a method of recovering lipase activity which comprises the steps of using a lipase derived from Thermomyces sp. and immobilized on a carrier, or a lipase powder composition which comprises a filter aid and the lipase derived from Thermomyces sp. and immobilized on a carrier which is crushed into the average particle size of 1 ?m or larger and smaller than 300 ?m in an esterification or transesterification reaction; and washing said lipase or lipase powder composition with triacylglycerol. According to this method, the decreased lipase activity can be effectively recovered.
    Type: Grant
    Filed: November 7, 2008
    Date of Patent: April 12, 2011
    Assignee: The Nisshin OilliO Group, Ltd.
    Inventors: Satoshi Negishi, Junko Suzuki, Yoshie Yamauchi
  • Publication number: 20110065163
    Abstract: The invention provides small enzyme-containing granules having an inorganic salt core and an enzyme-containing layer coated over the core, and methods for producing such granules. The majority of the enzyme granules are less than 300 ?m in diameter. The granules are suitable for incorporation into compositions such as cleaning, textile processing, and animal feed compositions.
    Type: Application
    Filed: February 11, 2009
    Publication date: March 17, 2011
    Inventors: Nathaniel T. Becker, Robert Christensen I., Mark S. Gerbert, Antti Pekka Vaha-Vahe
  • Patent number: 7901913
    Abstract: The invention is directed to DNA sequences from coryneform bacteria, particularly Corynebacterium glutamicum, which encode a protein having transaldolase enzymatic activity. The invention also encompasses processes for the fermentative production of L-amino acids using bacteria in which a gene encoding transaldolase is amplified.
    Type: Grant
    Filed: May 18, 2004
    Date of Patent: March 8, 2011
    Assignee: Evonik Degussa GmbH
    Inventors: Laurence Kieran Dunican, Rita Dunican, legal representative, Ashling McCormack, Cliona Stapelton, Kevin Burke, Bettina Mockel
  • Publication number: 20110027252
    Abstract: Variants of Bacillus sp. no. 707 alpha amylase are provided that are produced more efficiently and thus more economically. Higher fermentation yields are achieved through introducing amino acid variations that promote solubility of the variant in a fermentation broth. Increased solubility allows more enzyme to remain in solution after expression in a host cell. This in turn increases the efficiency with which the expressed variant enzyme can be recovered from the fermentation broth.
    Type: Application
    Filed: May 29, 2008
    Publication date: February 3, 2011
    Inventors: Wolfgang Aehle, Neelam S. Amin
  • Publication number: 20100272639
    Abstract: Polysaccharide nanoparticles that are particularly useful in for example drug and agent delivery, tissue-specific targeting, for medical imaging and diagnosis, as well as modifiers of physico-chemical properties. The nanoparticles can be highly-branched glucose homopolymers and can be characterized by a uniform spherical shape. They are monodisperse, hydrophilic and produce low solution viscosities. The nanoparticles are non-toxic, biocompatible and biodegradable. Also, the process of isolation of said polysaccharide nanoparticles from various organisms including, but not limited to, microorganisms such as bacteria and yeasts. Also provided are methods for chemical conjugation of the polysaccharide nanoparticles with various agents. Also provided are examples of use of the polysaccharide nanoparticles and their derivatives as drug delivery systems and fluorescent di-agnostics.
    Type: Application
    Filed: December 19, 2008
    Publication date: October 28, 2010
    Inventor: John Robert Dutcher
  • Patent number: 7776574
    Abstract: The invention relates to a thrombolytic enzyme referred to as Thrombinase having a molecular weight of 31,000 to 32,000. Such a thrombolytic enzyme can be used for dissolving blood clots. The process comprises culturing a filtrate of Bacillus sphaericus sero type H5a 5b, removing the cell, subjecting the cell supernatant to filtration, salting out the retentate, subjecting the precipitate to dialysis, reprecipitating the precipitate and then reconstituting in buffer and finally decolourizing, purifying and dialyzing.
    Type: Grant
    Filed: May 20, 2004
    Date of Patent: August 17, 2010
    Assignees: National Research Development Corporation, Malladi Drugs & Pharmaceuticals Ltd.
    Inventors: Perurmadom Ramaiyer Mahadevan, Sita Mahadevan, legal representative, Subrahmanyam Chivukula Sekar, Sundaramurthy Suresh Babu
  • Publication number: 20100035325
    Abstract: The present invention discloses thermostable microcapsules comprising a semipermeable membrane, an aqueous core, one or more enzymes in the aqueous core, and a nucleic acid template for an enzyme-mediated reaction in the aqueous core. In one embodiment of the invention, the aqueous core contains one or more polymerases. Microcapsules of the invention can be used in amplification and sequencing reactions. In particular, the microcapsules of the present invention can be used for high-throughput sequencing.
    Type: Application
    Filed: August 6, 2008
    Publication date: February 11, 2010
    Applicant: Genome Corporation
    Inventor: Kevin Ulmer
  • Publication number: 20090317515
    Abstract: The present invention relates to novel solid enzyme formulations comprising mixtures of at least one salt-stabilized enzyme composition, at least one particulate support and at least one hydrophobic liquid. In addition, the invention relates to methods for producing such solid enzyme formulations and also animal feed, foods and food supplements which comprise such enzyme formulations.
    Type: Application
    Filed: March 9, 2007
    Publication date: December 24, 2009
    Applicant: BASF SE
    Inventors: Markus Lohscheidt, Roland Betz, Jörg Braun, Wolf Pelletier
  • Patent number: 7632799
    Abstract: The present invention relates to a process for preparing coated granules comprising the steps of: (a) providing a core unit comprising an active component (b) contacting the core unit with a liquid dispersion comprising a solvent, a dissolved salt and solid dispersed particles wherein the solid particles constitute at least 10% w/w of the total dry matter of the dispersion (c) evaporating the solvent of the liquid dispersion to leave salt and solid particles coated onto the core unit.
    Type: Grant
    Filed: May 26, 2006
    Date of Patent: December 15, 2009
    Assignee: Novozymes A/S
    Inventors: Poul Bach, Ole Simonsen
  • Publication number: 20090291483
    Abstract: The invention relates to an enzyme-containing granule comprising a core unit and a shell unit, wherein the core unit comprises the enzyme and is enclosed in a shell unit which is substantially enzyme-free, the ratio between the diameter of the granule and the diameter of the core unit being at least 1.1. Also processes for producing such granules and use of the granules are disclosed.
    Type: Application
    Filed: August 3, 2009
    Publication date: November 26, 2009
    Applicant: Novozymes A/S
    Inventor: Poul Bach
  • Patent number: 7611701
    Abstract: A process for the preparation of an enzyme-containing granulate is disclosed where an aqueous enzyme-containing liquid is mixed with an edible carbohydrate-based solid carrier, such as starch, mechanically processed into granules, and subsequently dried. This enzyme granulate is suitable for the manufacture of animal feed compositions by mixing feed ingredients with the granulate, treating with steam and pelleting. The compositions optimally show improved enzyme stability during the pelleting process.
    Type: Grant
    Filed: October 25, 2002
    Date of Patent: November 3, 2009
    Assignee: BASF Aktiengesellschaft
    Inventors: Rudolph Carolus Maria Barendse, Gabriel Marinus Henricus Meesters, Hans-Peter Harz
  • Publication number: 20090226560
    Abstract: The present application relates to a steam treated pelletized feed composition comprising a granule comprising a core and a coating wherein the core comprises an active compound and the coating comprises a salt.
    Type: Application
    Filed: February 18, 2009
    Publication date: September 10, 2009
    Applicant: Novozymes A/S
    Inventors: Erik Marcussen, Flemming Borup, Ole Simonsen, Erik Kjaer Markussen
  • Publication number: 20090226561
    Abstract: The present application relates to a steam treated pelletized feed composition comprising a granule comprising a core and a coating wherein the core comprises an active compound and the coating comprises a salt.
    Type: Application
    Filed: February 18, 2009
    Publication date: September 10, 2009
    Applicant: Novozymes A/S
    Inventors: Erik Marcussen, Flemming Borup, Ole Simonsen, Erik Kjaer Markussen
  • Publication number: 20090197316
    Abstract: Protein particles are prepared by causing or allowing protein molecules dispersed in a liquid medium at a concentration of 8 mg.mL-1 or greater to react in the presence of a zero-length crosslinker, so as to produce protein particles comprising protein molecules that are covalently bonded together. The protein particles may be produced with sizes in the sub-micron range with closely defined sizes and size distributions. The particles have applications in many fields, but are useful inter alia for the delivery of therapeutic agents and other agents, eg imaging contrast agents, to the body.
    Type: Application
    Filed: July 13, 2007
    Publication date: August 6, 2009
    Applicants: Novozymes Biopharma UK Limited, Upperton Limited
    Inventors: Richard Alan Johnson, John Rodney Woodrow
  • Publication number: 20090075349
    Abstract: The present invention discloses a method of recovering lipase activity which comprises the steps of using a lipase derived from Thermomyces sp. and immobilized on a carrier, or a lipase powder composition which comprises a filter aid and the lipase derived from Thermomyces sp. and immobilized on a carrier which is crushed into the average particle size of 1 ?m or larger and smaller than 300 ?m in an esterification or transesterification reaction; and washing said lipase or lipase powder composition with triacylglycerol. According to this method, the decreased lipase activity can be effectively recovered.
    Type: Application
    Filed: November 7, 2008
    Publication date: March 19, 2009
    Applicant: The Nisshin OilliO Group, Ltd.
    Inventors: Satoshi NEGISHI, Junko Suzuki, Yoshie Yamauchi
  • Patent number: 7419947
    Abstract: The present invention relates to a granule comprising a core and a coating, wherein the core comprises an active compound, and the coating comprises filaments prepared from atomizing a liquid coating composition having the property that liquid coating composition forms filaments upon atomization.
    Type: Grant
    Filed: March 26, 2003
    Date of Patent: September 2, 2008
    Assignee: Novozymes A/S
    Inventors: Poul Bach, Ole Simonsen