Abstract: This invention provides nucleic acid sequences and characterization of the gene cluster responsible for the biosynthesis of the enediyne C-1027 (produced by Streptomyces globisporus). Methods are provided for the biosynthesis of enediynes, enediyne analogs and other biological molecules.

Abstract: The invention is directed to nucleic acid sequences which encode polypeptides having PhzO activity, namely, the ability to convert phenazine-1-carboxylic acid to a 2-hydroxylated phenazine, and isolated polypeptides having this activity. The invention is also directed to recombinant nucleic acid molecules, vectors, and host cells including the nucleic acid sequences as well as methods for producing and using the polypeptides, including expression in bacterial or plant cells to inhibit fungal pathogens.

Abstract: The present invention is a substantially purified sortase-transamidase enzyme from Gram-positive bacteria, such as Staphylococcus aureus.

Abstract: According to the present invention, the polypeptide of theobromine synthase derived from coffea arabica and the gene encoding said polypeptide are provided. As theobromine synthase participates in biosynthesis of caffeine, caffeineless coffee would be obtained by preparing a transformed plant, wherein expression of gene encoding said enzyme is inhibited.

Abstract: A fusion polypeptide comprising a protein of interest which has a reduced half-life of expression, and a nucleic acid molecule encoding the fusion polypeptide, are provided.

Abstract: Novel cytidine deaminase-like polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated, full-length cytidine deaminase-like proteins, the invention further provides isolated cytidine deaminase-like fusion proteins, antigenic peptides, and anti-cytidine deaminase-like antibodies. The invention also provides cytidine deaminase-like nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which an cytidine deaminase-like gene has been introduced or disrupted. Diagnostic, screening, and therapeutic methods utilizing compositions of the invention are also provided.

Abstract: The luxA and luxB genes from P. luminescens which encode for the luciferase protein of the bacterial luciferase system were modified to generate codon-optimized versions that are optimized for expression in mammalian cells. The codon-optimized bacterial luciferase enzyme system genes of the invention can be used to develop a mammalian bioluminescence bioreporter useful in various medical research and diagnostics applications.

Abstract: Phenylalanine hydroxylase deficiency in a subject is corrected by administering to the subject rAAV-based vectors that include a sequence encoding functional phenylalanine hydroxylase. Ribozymes are used to reduce expression of defective phenylalanine hydroxylase in a cell.

Abstract: By using a bioluminescence, a method for detection of a monovalent cation is provided and the method is a high sensitive and simple method. According to the present invention, an amino acid sequence of symplectin and a base sequence encoding the protein are provided. Symplectin is a photoprotein derived from Symplectoteuthis oualaniensis (okinawan squid). By using the photoprotein of the present invention, a monovalant cation can be detected by luminescence in the presence of a chromophore.

Abstract: The present invention relates to P450 enzymes and nucleic acid sequences encoding P450 enzymes in Nicotiana, and methods of using those enzymes and nucleic acid sequences to alter plant phenotypes.

Abstract: The present invention provides a novel fructosyl amino acid oxidase that is excellent in thermal stability. Moreover, the present invention provides a fructosyl amino acid oxidase gene encoding the novel fructosyl amino acid oxidase, a recombinant DNA in which the gene is inserted into a vector DNA, a transformant or transductant containing this gene, and a process for producing the novel fructosyl amino acid oxidase, which comprises culturing the transformant or transductant in a medium and collecting the novel fructosyl amino acid oxidase from the culture product.

Abstract: Mutants of leucine dehydrogenase sequences, formate dehydrogenase sequences and galactose oxidase sequences are provided. An amino acid sequence that is a mutant of a leucine dehydrogenase sequence as described in SEQ ID 2, or its substantial equivalent, contains at least one mutation selected from the group consisting of F102S, V33A, S351T, N145S and like mutations in subsantially equivalent sequences. An amino acid sequence that is a mutant of a formate dehydrogenase sequence as described in SEQ ID 1, or its substantial equivalent, contains at least one mutation selected from the group consisting of D195S, Y196H, K356T and like mutations in subsantially equivalent sequences.

Abstract: The present invention provides new recombinantly produced vanadium haloperoxidases. The enzymes are useful in a number of industrial applications.

Abstract: The present invention relates to novel human BMP polypeptides and isolated nucleic acids containing the coding regions of the genes encoding such polypeptides. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human BMP polypeptides. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating disorders related to these novel human BMP polypeptides.

Abstract: Novel pro-drugs and methods for their use to alter the growth and biological characteristics of living cells, tissues, or whole organisms are described. The methods allow for selective activation of the pro-drugs at or near transformant host cells expressing a gene for an enzyme that activates the pro-drugs. Pro-drugs according to a preferred embodiment of the invention are conjugates of a bioactive compound and a chemical group that is capable of being cleaved from the bioactive compound by action of an enzyme. Methods according to this invention include, (a) introducing into targeted cells a gene encoding an enzyme and (b) administering a pro-drug, wherein the enzyme releases the pro-drug from conjugation. In a preferred embodiment of the invention, the gene encoding the enzyme is a marker gene.

Abstract: The present invention provides endothelial nitric oxide synthase (eNOS) polypeptide mutants, and polynucleotides encoding such polypeptide mutants, useful for gene therapy. In particular, the invention provides eNOS polypeptide mutants having one or more mutations in an amino acid sequence corresponding to a functional domain of a mammalian eNOS. More particularly, the invention provides eNOS polypeptide mutants having at least one mutation at a position corresponding to an amino acid residue in a calmodulin-binding site that is phosphorylated in mammalian cells, where the mutation is not an amino acid substitution to Ala or Asp in an eNOS polypeptide mutant having a single mutation that is at the phosphorylation site; and to polynucleotides encoding such polypeptide mutants. The present invention further provides prophylactic, diagnostic, and therapeutic methods of using such eNOS polypeptide mutants and polynucleotides.

Abstract: The present invention is directed to arginine deiminase modified with polyethylene glycol, to methods of treating cancer, and to methods of treating and/or inhibiting metastasis.

Abstract: The present invention provides a novel L-glutamate oxidase, a gene encoding the enzyme, and a method for producing the enzyme. By use of a gene encoding the enzyme, L-glutamate oxidase can be readily prepared at low costs through a recombinant DNA technique.

Abstract: A reductase reducing keto-nicotianamine into 2′-deoxymugineic acid in the course of the biosynthesis of mugineic acids participating in the iron-acquisition mechanism in plants; a gene encoding this enzyme; a plant having a enhanced tolerance to iron-deficiency owing to the above gene transferred thereinto; and enzyme kits and gene kits for constructing a plant having a enhanced tolerance to iron-deficiency which comprise enzymes for biosynthesizing mugineic acids participating in the iron-acquisition mechanism in plants or genes encoding these enzymes.

Abstract: The invention relates to compositions containing a polynucleotide encoding for a reporter gene, a selectable marker and a regulatory element, that provide a method for imaging cells in vivo.

Abstract: The present invention relates to:

Abstract: Because the protein of the present invention has in its amino acid sequence a homology with, for example. ribonucleotide reductase, the protein of the present invention, its DNA, antibodies to these and the like are useful in the prevention, treatment and diagnosis, etc. of cancer.

Abstract: There is disclosed an expression vector utilizing an internal polyadenylation signal. The internal polyadenylation signal is inserted between a DNA encoding a protein of interest and a DNA encoding a selectable marker, and allows a single promoter to generate both monocistronic messages and dicistronic messages. Similar, multicistronic vectors can also be prepared. Also disclosed are methods of using the expression vector utilizing an internal polyadenylation signal, host cells transfected therewith, stable pools of cells transfected with an expression vector utilizing an internal polyadenylation signal, and clones of such transfected cells.

Abstract: An objective of the present invention is to provide DNAs encoding novel plant proteins having a gibberellin (GA) 2-oxidation activity. Another objective is to modify plant height by utilizing these DNAs for regulating the gibberellin content.

Abstract: Novel transcription transactivator protein of the CITED family, designated HCITEDX. Nucleic acids encoding the protein and uses of the protein are also provided.

Abstract: This invention provides materials and procedures for the delivery of selected strains of bacteria and/or oxalate-degrading enzymes to the intestinal tracts of persons and/or domestic or zoological animals who are at increased risk for oxalate related disease because they have lost, or have inadequate concentrations of these bacteria. The administration of these bacteria and/or the relevant enzyme removes oxalate from the intestinal tract and thus reduces the amount of oxalate available for absorption and reduces the risk for oxalate related disease.

Abstract: A reporter system reflecting the transport process that transports GPI-anchored proteins to the cell wall was constructed and compounds inhibiting this process were discovered. Further, genes conferring resistance to the above compounds were identified and methods of screening for compounds that inhibit the activity of the proteins encoded by these genes were developed.

Abstract: The present invention relates to methods for stimulating root growth and/or enhancing the formation of lateral or adventitious roots and/or altering root geotropism comprising expression of a plant cytokinin oxidase or comprising expression of another protein that reduces the level of active cytokinins in plants or plant parts. The invention also relates to novel plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising said sequences. The invention also relates to the use of said sequences for improving root-related characteristics including increasing yield and/or enhancing early vigor and/or modifying root/shoot ratio and/or improving resistance to lodging and/or increasing drought tolerance and/or promoting in vitro propagation of explants and/or modifying cell fate and/or plant development and/or plant morphology and/or plant biochemistry and/or plant physiology.

Abstract: A composition for use in the treatment of lesions of the human or animal body comprises xanthine oxidoreductase and a pharmaceutically acceptable electron donor system. The composition can accelerate wound healing, especially in a hypoxic environment.

Abstract: The invention relates to protein conjugates, methods, vectors, proteins and DNA for producing them, their use, and medicaments and vaccines containing a certain quantity of said protein conjugates. According to the invention, supramolecular particles are produced that represent one or more different, randomly selectable structural units in a large number on the surface of an individual, approximately spherical protein molecule. Icosahedral lumazine synthases are used as carrier proteins for peptides or proteins. A DNA fragment that encodes a peptide molecule is fused with a DNA fragment that encodes an icosahedral lumazine synthase by molecular-biological methods. Said DNA fragment is inserted into a cloning vector and transformed with an appropriate host strain. A polypeptide is expressed by gene expression. If certain peptide structures are used as the fusion partners, a post-translational change of said structures can be observed in the host strain.

Abstract: The present invention relates to an enzyme immunoassay method for assaying calcium ion, including putting calcium-dependent glutamate dehydrogenase, namely glutamate dehydrogenase (GLDH) never expressing its activity in the absence of calcium ion, in contact to the calcium ion in a sample to assay the enzyme activity of the GLDH, the activity changing depending on the calcium ion concentration in the sample; and the invention also relates to an assay reagent for use in the same. Because the reagent is very stable in a solution state under storage, the reagent can be incorporated in a liquid reagent. Thus, the calcium in a biological material or other samples can be assayed in a simple and accurate manner.

Abstract: Nitroreductases, and genes encoding the same, are provided that demonstrate preferential catalytic conversion of the alkylating agent CB1954 into its highly cytotoxic 4-hydroxylamine (4HX) derivative, this derivative demonstrating anticarcinoma properties. Accordingly, the catalytic activity of the nitroreductase enzymes of the present invention may be employed to achieve catalysis of CB1954 into its cytotoxic derivative in a site-directed manner, such as by Directed-Enzyme Prodrug Therapy (DEPT).

Abstract: The present invention is directed to a nucleotide sequence encoding D-amino oxidase (D-AAO, EC 1.4.3.3) from Arthrobacter protophormiae. This may be inserted into an expression and used to transform a microbial host. The transformed microbial hosts ecomically produce D-AAO which may be used to oxidize D-amino acids.

Abstract: The invention provides isolated nucleic acids molecules, designated 97316 nucleic acid molecules, which encode novel amine oxidase family members. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing 97316 nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a 97316 gene has been introduced or disrupted. The invention still further provides isolated 97316 proteins, fusion proteins, antigenic peptides and anti-97316 antibodies. Diagnostic and therapeutic methods utilizing compositions of the invention are also provided.

Abstract: The present invention is directed to compositions comprising an angiogenesis inhibitor coupled to a therapeutic or diagnostic agent. In a specific embodiment, the composition is a fusion gene or fusion gene product encoding the angiogenesis inhibitor coupled to a therapeutic or diagnostic agent. In a particular embodiment, the composition is used for methods to treat angiogenesis-related diseases, such as cancer.

Abstract: The invention provides methods of discovering nitroreductases and biocatalytic methods for reducing compounds comprising a nitro group.

Abstract: This invention features a nucleic acid expression vector that includes a bicistronic coding unit that comprises a first segment that encodes a human tissue urokinase plasminogen activator protein and a second segment that encodes an amplifiable dominant selectable marker (e.g., dihydrofolate reductase); and a promoter (e.g., a cytomegalovirus promoter) operably linked to the bicistronic coding unit.

Abstract: The present invention provides a polynucleotide or polynucleotides encoding Oplophorus luciferase which is composed of 19 kDa and 35 kDa proteins, or the 19 kDa photoprotein, the recombinant secretional Oplophorus luciferase or the 19 kDa photoprotein encoded by the polynucleotide(s), an expression vector containing the polynucleotide(s) and a host transformed with the vector.

Abstract: The invention relates to a mutant protein comprising at least a fragment of a mutant dimethylarginine dimethylaminohydrolase (DDAH) enzyme, wherein the fragment possesses an affinity for asymmetric N,N-dimethyl arginine (ADMA) and/or L,N-monomethylarginine (LNMMA), which exists at lower plasma levels than ADMA, and is deficient in hydrolyzing ADMA or LNMMA to citrulline, releasing citrulline, or both.

Abstract: The present invention relates to a storage-stable liquid formulation comprising a laccase comprising (i) a laccase, (ii) at least one polyalcohol, which formulation has a pH which is more alkaline than the pH optimum of the laccase. It is also an object of the invention to provide a method for improving the storage-stability of liquid formulations comprising a laccase and the use of said liquid formulations for a personal care application or for bleaching or for textile applications such as dyeing of fabrics.

Abstract: Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50° C., cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.

Abstract: The present invention provides MICAL and MICAL-Like polypeptides and polynucleotides. Also provided are methods that for identifying agents that affect axon growth and placement. Furthermore, provided herein are methods for affecting axon growth and placement.

Abstract: The invention relates to polypeptides from phytopathogenic fungi with the biological activity of an inosine monophosphate dehydrogenase, to nucleic acids encoding them, to the use of the polypeptides and nucleic acids for identifying modulators of the polypeptides, to methods for identifying such modulators, and to the use of these modulators as fungicides.

Abstract: An isolated DNA fragment encoding a protein with CAD activity wherein the protein is clavulanic acid dehydrogenase (CAD) from S. clavuligerus, with the proviso that the isolated DNA fragment is not: (a) the 13.1 kb BglII to SphI fragment of S. clavuligerus; (b) the 10.8 kb EcoRI to BamHI fragment of S. clavuligerus; (c) the 8.8 kb BclI to BclII fragment of S. clavuligerus; (d) the 2.5 kb BglII to BglII fragment of S. clavuligerus; (e) the 60 kb BglII to BamHI fragment of S. clavuligerus; or (f) the 18.0 BclI to SphI fragment of S. clavuligerus.

Abstract: A gene has been isolated from a Rhodococcus sp. encoding an aldoxime dehydratase enzyme useful for the conversion of aldoxime substrates to nitrilases and other downstream intermediates. The gene has been cloned into a recombinant host and expressed.

Abstract: The nucleotide and polypeptide sequences of two novel human flavin-containing monooxygenase (FMO) enzymes are presented: hFMO2 and hFMOx. Vectors and host cells for cloning and/or expression of hFMO2 and hFMOx are described, as are methods of preparing hFMO2 and hFMOx polypeptides. Also included are methods for selecting compounds, diagnosing predisposition to pathologies and/or deficiencies related to FMO's, and pharmaceutical compositions containing compounds for treating and/or preventing these pathologies.

Abstract: The present invention discloses arginine deiminase that is genetically modified for more efficient manufacturing and processing. The present invention discloses recombinant DNA molecules and vectors and other therapeutic and pharmaceutical compositions. The present invention also discloses methods for preparing modified arginine deiminase as well as methods of treating cancer and other disease states using modified arginine deiminase.

Abstract: The methods and materials disclosed herein are directed to glyphosate herbicide tolerance in plants. In particular, the isolation of a glyphosate resistant EPSP synthase coding sequence and its regulatory elements from Eleusine indica. The coding sequence and regulatory sequences are useful to genetically engineer plants for tolerance to glyphosate herbicide.

Abstract: The invention relates to a process for the fermentative preparation of L-threonine in which an L-threonine-producing microorganism of the Enterobacteriaceae family is cultured by the feed process, and a portion of the fermentation broth is then separated off in order to be utilized for inoculation of further media.

Abstract: The present invention provides a novel polypeptide producing (S)-N-benzyl-3-pyrrolidinol, a DNA coding for it and a method of using them.