Oxidoreductase (1. ) (e.g., Luciferase) Patents (Class 435/189)
  • Patent number: 10378023
    Abstract: The invention relates to biotechnology and provides novel recombinant DNA molecules and engineered proteins for conferring tolerance to protoporphyrinogen oxidase-inhibitor herbicides. The invention also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.
    Type: Grant
    Filed: August 4, 2016
    Date of Patent: August 13, 2019
    Assignee: Monsanto Technology LLC
    Inventors: Artem G. Evdokimov, Clayton T. Larue, Farhad Moshiri, Joel E. Ream, Xuefeng Zhou
  • Patent number: 10377722
    Abstract: An object of the present invention is to provide a novel method capable of producing rosuvastatin calcium and intermediates therefor efficiently, inexpensively and with high purity. The present invention provides a method of efficiently producing rosuvastatin calcium and intermediates therefor having a high purity at an industrial scale, without using an extremely low temperature reaction or a special asymmetric catalyst.
    Type: Grant
    Filed: May 19, 2017
    Date of Patent: August 13, 2019
    Assignee: API CORPORATION
    Inventors: Naoyuki Watanabe, Tomoko Maeda, Yasumasa Dekishima, Hiroshi Kawabata, Masaki Nagahama, Kosuke Ito
  • Patent number: 10370677
    Abstract: The invention relates to biotechnology and provides novel recombinant DNA molecules and engineered proteins for conferring tolerance to protoporphyrinogen oxidase-inhibitor herbicides. The invention also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.
    Type: Grant
    Filed: July 29, 2016
    Date of Patent: August 6, 2019
    Assignee: Monsanto Technology LLC
    Inventors: Artem G. Evdokimov, Clayton T. Larue, Farhad Moshiri, Joel E. Ream, Xuefeng Zhou
  • Patent number: 10358631
    Abstract: The disclosure relates to engineered ketoreductase polypeptides and processes of using the polypeptides for production of phenylephrine.
    Type: Grant
    Filed: November 14, 2017
    Date of Patent: July 23, 2019
    Assignee: Codexis, Inc.
    Inventors: Oscar Alvizo, Steven J. Collier, Hans-Georg Joerg Hennemann, Seong Ho Oh, Wenjuan Zha
  • Patent number: 10264807
    Abstract: The invention relates to fusarium toxin-cleaving polypeptide variants of a fusarium toxin carboxyl esterase with the SEQ ID NO:46. Each of the polypeptide variants has an amino acid sequence shortened by 47 amino acids at the N terminus, and the amino acid sequences have at least 70%, preferably 80%, in particular 100%, sequence identity, namely SEQ ID NO:1, to the amino acid sequence portion 48-540 of the SEQ ID NO:46. The invention also relates to isolated polynucleotides which code for the polypeptide variants, to a fusarium toxin-cleaving additive containing at least one polypeptide variant and optionally at least one auxiliary agent, to the use of the polypeptide variants or the additive, and to a method for hydrolytically cleaving at least one fusarium toxin.
    Type: Grant
    Filed: February 24, 2015
    Date of Patent: April 23, 2019
    Assignee: ERBER AKTIENGESELLSCHAFT
    Inventors: Markus Aleschko, Corinna Kern, Dieter Moll, Eva Maria Binder, Gerd Schatzmayr
  • Patent number: 10253303
    Abstract: The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.
    Type: Grant
    Filed: September 10, 2018
    Date of Patent: April 9, 2019
    Assignee: Codexis, Inc.
    Inventors: Fabien Louis Cabirol, Haibin Chen, Anupam Gohel, Steven J. Collier, Derek J. Smith, Birgit Kosjek, Jacob Janey
  • Patent number: 10246687
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl (1,3-oxazolidin-3-yl))-1-(4-fluorophenyl) pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.
    Type: Grant
    Filed: July 10, 2018
    Date of Patent: April 2, 2019
    Assignee: Codexis, Inc.
    Inventors: Emily Mundorff, Erik Jan De Vries
  • Patent number: 10239922
    Abstract: The present disclosure pertains to methods of producing recombinant peptides that contain between 10 and 200 amino acid residues using novel carrier proteins derived from superfolder green fluorescent protein and its mutants.
    Type: Grant
    Filed: June 29, 2017
    Date of Patent: March 26, 2019
    Assignee: Suzhou Kunpeng Biotech Co., Ltd.
    Inventors: Wenshe Liu, Wei Wan
  • Patent number: 10202627
    Abstract: An object of the present invention is to provide enzymes and a DNA encoding the enzymes that are involved in biosynthesis of trehangelin which has the potential to be a therapeutic agent for photosensitivity disorder and cosmetics, and to provide a method for producing trehangelin by utilizing the enzymes and a recombinant microorganism. The present invention is directed to a protein having an amino acid sequence of SEQ ID NO: 3, 5, 7 or 9, or a protein having an amino acid sequence of SEQ ID NO: 3, 5, 7 or 9 in which one to several amino acids are deleted, substituted, added and/or inserted or an amino acid sequence having 60% or more homology with the amino acid sequence of SEQ ID NO: 3, 5, 7 or 9 and having an enzyme activity involved in biosynthesis of trehangelin; and a DNA encoding said protein.
    Type: Grant
    Filed: February 28, 2017
    Date of Patent: February 12, 2019
    Assignees: The Kitasato Institute, Nagase & Co., Ltd.
    Inventors: Satoshi Omura, Yoko Takahashi, Takuji Nakashima, Yuki Inahashi
  • Patent number: 10202585
    Abstract: Improved matrix or hydrogel that is formed by enzymatic crosslinking of polymers wherein the crosslinking enzyme molecules have been modified for the purpose of improving the crosslinking density, mechanical properties, or other properties of the matrix, and/or to provide improved control over the rate and/or extent of crosslinking, wherein the enzyme molecules are modified to alter the perceived volume of the enzyme molecules in the crosslinked matrix being formed. Methods of production and of use are also provided.
    Type: Grant
    Filed: April 25, 2015
    Date of Patent: February 12, 2019
    Assignee: LIFEBOND LTD
    Inventors: Guy Tomer, Orahn Preiss-Bloom
  • Patent number: 10184116
    Abstract: A novel luciferase that distinct from conventional luciferase has been desired. A luciferase mutant comprising the amino acid sequence of SEQ ID NO: 2 substituted at tyrosine at the position of 138, and at least 3 positions selected from the group consisting of isoleucine at the position of 90, proline at the position of 115, glutamine at the position of 124, and asparagine at the position of 166.
    Type: Grant
    Filed: March 9, 2015
    Date of Patent: January 22, 2019
    Assignee: JNC CORPORATION
    Inventors: Satoshi Inouye, Junichi Sato
  • Patent number: 10100287
    Abstract: The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.
    Type: Grant
    Filed: September 21, 2017
    Date of Patent: October 16, 2018
    Assignee: Codexis, Inc.
    Inventors: Fabien Louis Cabirol, Haibin Chen, Anupam Gohel, Steven J. Collier, Derek J. Smith, Birgit Kosjek, Jacob Janey
  • Patent number: 10093905
    Abstract: The invention relates to ketoreductases and the use thereof. The ketoreductases of the invention are particularly useful for enzymatically catalyzing the reduction of ketones to chiral secondary alcohols.
    Type: Grant
    Filed: April 17, 2015
    Date of Patent: October 9, 2018
    Assignee: C-LEcta GmbH
    Inventors: Ramona Schmiedel, Andreas Vogel, Sabrina Koepke, Rico Czaja, Claudia Feller, Hedda Merkens, Kamila Rzeznicka, Daniel Schwarze, Thomas Greiner-Stoeffele, Andreas Petri, Marc Struhalla
  • Patent number: 10053673
    Abstract: The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
    Type: Grant
    Filed: April 4, 2017
    Date of Patent: August 21, 2018
    Assignee: Codexis, Inc.
    Inventors: Michael A. Crowe, Oscar Alvizo, Behnaz Behrouzian, Yong Koy Bong, Steven J. Collier, Anupam Gohel, Jagadeesh Mavinahalli, Naga K. Modukuru, Emily Mundorff, Derek J. Smith, Shiwei Song, Wan Lin Yeo
  • Patent number: 10047348
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl (1,3-oxazolidin-3-yl))-1-(4-fluorophenyl) pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.
    Type: Grant
    Filed: September 21, 2017
    Date of Patent: August 14, 2018
    Assignee: Codexis, Inc.
    Inventors: Emily Mundorff, Erik Jan De Vries
  • Patent number: 9988609
    Abstract: Fructosyl amino acid oxidase is provided, which has an amino acid sequence as shown in SEQID. No. 1 or fructosyl amino acid oxidase having a homology of more than 80% with this amino acid sequence, on a corresponding site of an amino acid selected from following (a) to (f), having one or more amino acid residues conducting a substitution, obtained fructosyl amino acid oxidase having a higher thermostability: (a) 59-site glutamic acid, (b) 98-site glutamic acid, (c) 225-site glycine, (d) 277-site lysine, (e) 285-site glutamic acid, and (f) 355-site aspartic acid. The method for preparing the above oxidase and the test kit containing the enzyme for determining glycated albumin are also provided.
    Type: Grant
    Filed: December 11, 2014
    Date of Patent: June 5, 2018
    Assignee: MEDICAL SYSTEM BIOTECHNOLOGY CO., LTD.
    Inventors: Bingde Zou, Jihua Zou, Yi Wang, Jianghua Jia
  • Patent number: 9976164
    Abstract: The present invention relates to a method for producing a protein of interest containing one or more disulfide bonds in its native state. The method comprises that a prokaryotic host cell is genetically engineered to express the protein of interest and a sulfhydryl oxidase in the cytoplasm of the host cell. The protein of interest is formed in a soluble form and contains disulfide bonds due to the presence of the sulfhydryl oxidase in the cytoplasm of said host cell. The present invention relates also to a prokaryotic host cell and a vector system for producing a protein of interest containing natively folded disulfide bonds.
    Type: Grant
    Filed: December 9, 2015
    Date of Patent: May 22, 2018
    Assignee: University of Oulu
    Inventor: Lloyd Ruddock
  • Patent number: 9963720
    Abstract: This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.
    Type: Grant
    Filed: August 1, 2016
    Date of Patent: May 8, 2018
    Assignee: California Institute of Technology
    Inventors: Frances Arnold, Peter Meinhold, Matthew W. Peters, Rudi Fasan, Mike M. Y. Chen
  • Patent number: 9951102
    Abstract: An isolated peptide or fusion having the peptide useful to interfere with the phosphorylation of Nox1, block or inhibit binding of NoxA1 activation domain (AD) to Nox1, and/or prevent or inhibit assembly of the p47phox/NoxA1 complex with Nox1, or a vector encoding the peptide or fusion thereof, and methods of using the peptide, fusion or vector are provided.
    Type: Grant
    Filed: April 17, 2015
    Date of Patent: April 24, 2018
    Assignee: University of Iowa Research Foundation
    Inventors: Francis J. Miller, Jr., Jennifer Streeter
  • Patent number: 9896674
    Abstract: Described herein are variants of H. jecorina CBH I, a Cel7 enzyme. The present invention provides novel cellobiohydrolases that have improved thermostability and reversibility.
    Type: Grant
    Filed: January 21, 2016
    Date of Patent: February 20, 2018
    Assignee: DANISCO US INC
    Inventors: Anthony Day, Frits Goedegebuur, Peter Gualfetti, Colin Mitchinson, Paulien Neefe, Mats Sandgren, Andrew Shaw, Jerry Stahlberg
  • Patent number: 9868941
    Abstract: A novel luciferase that is distinct from conventional luciferase has been desired. A luciferase mutant comprising an amino acid sequence in which glutamic acid at position 4, arginine at position 11, leucine at position 18 and valine at position 27 are substituted with other amino acids, in the amino acid sequence of SEQ ID NO: 2.
    Type: Grant
    Filed: April 14, 2015
    Date of Patent: January 16, 2018
    Assignee: JNC CORPORATION
    Inventors: Satoshi Inouye, Yuiko Miura, Junichi Sato
  • Patent number: 9861602
    Abstract: One embodiment described herein is related to methods and compositions, such as nutraceutical formulations and dietary supplements, comprising C16:1n7-palmitoleate or derivatives thereof. The methods and compositions comprising C16:1n7-palmitoleate, or derivatives thereof, safely and effectively prevent or mitigate manifestations of cardiovascular disease, including coronary artery disease and the accumulation of cholesterol or lipid deposits in the blood vessels of a subject.
    Type: Grant
    Filed: April 1, 2014
    Date of Patent: January 9, 2018
    Assignee: TERSUS LIFE SCIENCES, LLC
    Inventor: Jeffrey Green
  • Patent number: 9834802
    Abstract: Methods for the production of L-glufosinate (also known as phosphinothricin or (S)-2-amino-4-(hydroxy(methyl)phosphonoyl)butanoic acid) are provided. The methods comprise a two-step process. The first step involves the oxidative deamination of D-glufosinate to PPO (2-oxo-4-(hydroxy(methyl)phosphinoyl)butyric acid). The second step involves the specific amination of PPO to L-glufosinate, using an amine group from one or more amine donors. By combining these two reactions, the proportion of L-glufosinate in a mixture of L-glufosinate and D-glufosinate can be substantially increased.
    Type: Grant
    Filed: February 28, 2017
    Date of Patent: December 5, 2017
    Assignee: AgriMetis, LLC
    Inventors: Brian Michael Green, Michelle Lorraine Gradley
  • Patent number: 9828588
    Abstract: The present invention provides a stable cell line overexpressing a recombinant vitamin K-dependent (VKD) protein and both a recombinant vitamin K epoxide reductase (VKOR) and a recombinant vitamin K dependent gamma carboxylase (VKGC), wherein the amount of fully carboxylated VKD protein produced in the cell is increased 10% as compared to the amount of fully carboxylated VKD protein produced in the cell in the absence of the recombinant VKOR and the recombinant VKGC.
    Type: Grant
    Filed: September 25, 2014
    Date of Patent: November 28, 2017
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: Darrel W. Stafford, Tao Li
  • Patent number: 9820484
    Abstract: A microbial oil comprising dihomo-?-linolenic acid as a constituent fatty acid of an oil, the microbial oil has a content, in terms of a weight ratio of arachidonic acid relative to dihomo-?-linolenic acid (arachidonic acid/dihomo-?-linolenic acid) of less than 1/13. Preferably, the microbial oil has a triglyceride content of greater than or equal to 70% by weight, and a saturated fatty acid content of less than or equal to 40% by weight. Moreover, a lower alcohol ester of dihomo-?-linolenic acid or a free fatty acid of dihomo-?-linolenic acid obtained from the microbial oil is provided.
    Type: Grant
    Filed: December 4, 2014
    Date of Patent: November 21, 2017
    Assignee: NIPPON SUISAN KAISHA, LTD.
    Inventors: Seizo Sato, Takuro Fukae, Naomi Ohtsuka, Hideaki Yamaguchi, Rie Ikeda
  • Patent number: 9783859
    Abstract: A method of detecting and quantifying various enzymatic activities using a constructed artificial genetic circuit GESS (genetic enzyme screening system) for sensing phenolic compounds and a method of screening a trace of activities of target enzymes from a metagenome using the artificial genetic circuit, thereby securing target enzyme genes. When the method for screening and quantifying target enzymatic activity is used, useful genes can be screened from various genetic communities, including environmental or metagenomic libraries, at a single cell level in high throughput (million/day). Further, the sensitivity of the genetic circuit to phenol derivatives and the expression thereof can be controlled, and thus the genetic circuit can rapidly sense and quantify various enzymatic activities. Thus, the method can be advantageously used in the protein engineering technology for enzyme modification.
    Type: Grant
    Filed: June 8, 2010
    Date of Patent: October 10, 2017
    Assignee: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY
    Inventors: Seung Goo Lee, Eugene Rha, Su Lim Choi, Jae Jun Song, Jong Hyun Choi, Hee Sik Kim
  • Patent number: 9765373
    Abstract: The present invention relates to polypeptides having cellulolytic enhancing activity, catalytic domains, and carbohydrate binding domains, and polynucleotides encoding the polypeptides, catalytic domains, and carbohydrate binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, and carbohydrate binding domains.
    Type: Grant
    Filed: December 13, 2013
    Date of Patent: September 19, 2017
    Assignees: Novozymes A/S, Novozymes, Inc.
    Inventors: Kirk Schnorr, Tarana Shaghasi, Matt Sweeney
  • Patent number: 9732328
    Abstract: The present invention encompasses modified luciferases, methods for making modified luciferases, and assays utilizing modified luciferases. Modified luciferases of the invention show increased activity over wildtype luciferases and also show increased stability of signal. The present invention also encompasses multiplex assays utilizing multiple luciferases with different emission spectra.
    Type: Grant
    Filed: January 20, 2016
    Date of Patent: August 15, 2017
    Assignee: Targeting Systems
    Inventor: Rampyari Walia
  • Patent number: 9714432
    Abstract: The present invention provides use of a plant gene JAZ5a for improving drought-resistance of a plant. It further provides a method for improving the drought-resistance of a plant, comprising enhancing the expression or activity of Jaz5A in said plant.
    Type: Grant
    Filed: July 5, 2012
    Date of Patent: July 25, 2017
    Assignee: Keygene N.V.
    Inventor: Adrianus Johannes Van Tunen
  • Patent number: 9663772
    Abstract: The present invention relates to recombinant Trichoderma host cells producing Aspergillus fumigatus cellulolytic enzyme compositions and methods of producing and using the compositions.
    Type: Grant
    Filed: August 23, 2012
    Date of Patent: May 30, 2017
    Assignee: NOVOZYMES, INC.
    Inventors: Jeffrey Shasky, Brett McBrayer
  • Patent number: 9662515
    Abstract: The invention relates to a process for the production of 4-aminobutyric acid, also referred to as gamma-aminobutyric acid, in short GABA, to a process for the production of an extract comprising 4-aminobutyric acid, to a process for the preparation of a cosmetic formulation comprising 4-aminobutyric acid, and to the use of Cyanidium caldarium cells or cell components in cosmetic applications.
    Type: Grant
    Filed: July 20, 2012
    Date of Patent: May 30, 2017
    Assignee: EVONIK DEGUSSA GMBH
    Inventors: Tim Köhler, Jennifer Schild, Matthias Mentel, Peter Lersch, Mike Farwick, Christian Weitemeyer
  • Patent number: 9650625
    Abstract: A pectinase having improved thermostability is disclosed. The pectinase has a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of serine at position 181 with phenylalanine, methionine or leucine.
    Type: Grant
    Filed: October 26, 2016
    Date of Patent: May 16, 2017
    Assignee: DONGGUAN APAC BIOTECHNOLOGY CO., LTD.
    Inventors: Rey-Ting Guo, Ya-Shan Cheng, Jian-Wen Huang, Tzu-Hui Wu, Hui-Lin Lai, Cheng-Yen Lin, Tsung-Yu Ko
  • Patent number: 9644189
    Abstract: The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: May 9, 2017
    Assignee: Codexis, Inc.
    Inventors: Michael A. Crowe, Oscar Alvizo, Behnaz Behrouzian, Yong Koy Bong, Steven J. Collier, Anupam Gohel, Jagadeesh Mavinahalli, Naga K. Modukuru, Emily Mundorff, Derek J. Smith, Shiwei Song, Wan Lin Yeo
  • Patent number: 9631002
    Abstract: The present invention provides methods and compositions for the production of vitamin K dependent proteins.
    Type: Grant
    Filed: December 21, 2011
    Date of Patent: April 25, 2017
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: Jianke Tie, Darrel W. Stafford
  • Patent number: 9630988
    Abstract: The present invention provides a method for preparing a composition comprising highly concentrated antibodies by ultrafiltration in batch concentration mode having a first constant feed rate step and a second controlled feed rate step.
    Type: Grant
    Filed: August 31, 2012
    Date of Patent: April 25, 2017
    Assignees: Chugai Seiyaku Kabushiki Kaisha, Genentech, Inc.
    Inventors: Kelby Lau, Jean Bender, Saeko Tanaka, Rumiko Wakayama, Hidenari Yamada, Tomonori Isoda, Masayoshi Oh-Eda
  • Patent number: 9611503
    Abstract: The objective of this invention is to create a double thyA folA knockout Escherichia coli (E. coli) strain for antifolate screening against DHFR of malaria and other parasites. This strain is used together with a plasmid expressing DHFR-TS from the desired pathogenic organism, which constitutes an anti-DHFR assay against the pathogenic organism of interest. The benefit of this invention is that there is no interference from either host DHFR or trimethoprim, a bacterial DHFR inhibitor. This tool is easy to use and maintain. It provides quick and reliable results as compared with conventional anti-malarial and anti-parasitic assays. This invention should facilitate discovery of new anti-DHFR compounds against malaria and other parasitic diseases.
    Type: Grant
    Filed: February 9, 2012
    Date of Patent: April 4, 2017
    Assignee: National Science and Technology Development Agency
    Inventors: Nattida Suwanakitti, Sastra Chaotheing, Yongyuth Yuthavong, Sumalee Kamchonwongpaisan
  • Patent number: 9504271
    Abstract: A method for sterilizing microbial cells is provided. According to the method, microbial cells or a culture containing microbial cells are treated with a polyethylene glycol-based nonionic surfactant so that almost all of the microbial cells are sterilized while the enzyme activity expressed in the microbial cells is maintained at a high level. A method for sterilizing microbial cells and a material containing the sterilized microbial cells, in which the microbial cells are sterilized using a polyethylene glycol-based nonionic surfactant, can be used for foods so that the microbial cells are sterilized to be used in food production. Further, a material containing sterilized microbial cells can be used in processes for preparing tagatose, in which Corynebacterium genus microbial cells that produce Galactose and/or Arabinose isomerase are sterilized using a polyethylene glycol-based nonionic surfactant.
    Type: Grant
    Filed: June 20, 2014
    Date of Patent: November 29, 2016
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Jin Ha Kim, Young Mi Lee, Seong Bo Kim, Taek Beom Kim, Yang Hee Kim, Seung Won Park
  • Patent number: 9506104
    Abstract: There has been a demand for a codon-optimized gene for the mutated catalytic domain of Oplophorus luciferase, which is capable of efficiently expressing a protein both in a cultured animal cell and Escherichia coli. There has also been a demand for a substrate coelenterazine analog showing a higher activity than that of native 19 kDa protein. The invention provides a polynucleotide comprising a polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2. According to the invention, bis-coelenterazine is used as a substrate coelenterazine analog suitable for the photoprotein encoded by the polynucleotide comprising the polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2.
    Type: Grant
    Filed: June 24, 2016
    Date of Patent: November 29, 2016
    Assignee: JNC CORPORATION
    Inventors: Satoshi Inouye, Junichi Sato
  • Patent number: 9506042
    Abstract: The present invention provides a novel glucose dehydrogenase that has excellent substrate specificity, specific activity, thermal stability, and the like, and that is suitable for use in SMBG sensors. The present invention provides a purified polypeptide comprising an amino acid sequence having at least 80% identity to the sequence of SEQ ID NO: 1 and having glucose dehydrogenase activity.
    Type: Grant
    Filed: December 29, 2014
    Date of Patent: November 29, 2016
    Assignee: Toyobo Co., Ltd.
    Inventors: Yosuke Sumida, Rie Hirao, Yuu Utashima, Hiroaki Kitazawa, Hiroshi Aiba, Takahide Kishimoto, Shusaku Yanagidani
  • Patent number: 9476036
    Abstract: The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.
    Type: Grant
    Filed: August 23, 2012
    Date of Patent: October 25, 2016
    Assignee: Novozymes, Inc.
    Inventors: Jeffrey Shasky, Amanda Fischer, Suchindra Maiyuran
  • Patent number: 9453237
    Abstract: The present invention relates to means and methods for modifying the expression of a component in the carotenoid biosynthetic pathway to modify the production of carotenoid towards accumulation of at least one of phytoene, phytofluene, zeta carotene or combinations thereof. The present invention further relates to genetically altered organisms having elevated content of these carotenoids. The present invention further relates to genetically altered organisms having elevated content of these carotenoids, particularly in chromoplast-containing cells.
    Type: Grant
    Filed: March 9, 2011
    Date of Patent: September 27, 2016
    Assignee: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd.
    Inventors: Joseph Hirschberg, Dani Zamir
  • Patent number: 9441236
    Abstract: Transgenic plants, and a method for making the same, wherein genes encoding the enzyme luciferase and its corresponding substrate luciferin are incorporated into a native plant genome. Once transformed into plant cells, these genes may be regulated such that under certain endogenous or exogenous conditions, their expression in the mature plant results in bioluminescence. Different luciferin/luciferase complexes and/or mechanisms of regulation may be utilized for these transgenic plants, depending on a variety of factors such as plant species and the circumstances under which a biolmuniescent reaction is desired. Phototransformation may be utilized to vary the wavelength of light emitted from the mature plant.
    Type: Grant
    Filed: September 25, 2014
    Date of Patent: September 13, 2016
    Inventor: Bruce Eric Hudkins
  • Patent number: 9422530
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl (1,3-oxazolidin-3-yl))-1-(4-fluorophenyl) pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe.
    Type: Grant
    Filed: August 12, 2015
    Date of Patent: August 23, 2016
    Assignee: Codexis, Inc.
    Inventors: Emily Mundorff, Erik Jan De Vries
  • Patent number: 9416350
    Abstract: An object of the present invention is to provide a method for converting the coenzyme dependency of enzymes of the medium-chain dehydrogenase/reductase (MDR) family. A further object of the present invention is to provide enzyme variants of the MDR family whose coenzyme dependency is converted by the conversion method and a method for enzymatically producing optically active alcohols using the enzymes. The present inventors developed a novel enzyme conversion method for converting the coenzyme dependency of enzymes of the MDR family, rationally designed enzyme variants that are altered by the enzyme conversion method to be able to use NADPH as a coenzyme from a useful enzyme of the MDR family that uses NADH as a coenzyme, and actually provide variants having such an ability.
    Type: Grant
    Filed: June 27, 2012
    Date of Patent: August 16, 2016
    Assignee: KANEKA CORPORATION
    Inventors: Shinichi Yoshida, Shunichi Taira, Masakatsu Nishihachijyo, Masutoshi Nojiri, Shigeru Kawano
  • Patent number: 9404145
    Abstract: There has been a demand for a codon-optimized gene for the mutated catalytic domain of Oplophorus luciferase, which is capable of efficiently expressing a protein both in a cultured animal cell and Escherichia coli. There has also been a demand for a substrate coelenterazine analog showing a higher activity than that of native 19 kDa protein. The invention provides a polynucleotide comprising a polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2. According to the invention, bis-coelenterazine is used as a substrate coelenterazine analog suitable for the photoprotein encoded by the polynucleotide comprising the polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 2.
    Type: Grant
    Filed: March 11, 2016
    Date of Patent: August 2, 2016
    Assignee: JNC CORPORATION
    Inventors: Satoshi Inouye, Junichi Sato
  • Patent number: 9394513
    Abstract: For the purpose of providing a method of safely and inexpensively producing a fermented plant extract containing an immunopotentiator at a high concentration, the method for fermentation and culture of the present invention ferments a plant component such as wheat flour using Pantoea agglomerans which is a gram negative bacterium which lives in a symbiotic relationship with a plant such as wheat and apple. It becomes possible to remarkably augment an immunopotentiation action which the plant has. In addition, these are not contaminated with impurities derived from animal components, and thus these are highly safe.
    Type: Grant
    Filed: November 15, 2011
    Date of Patent: July 19, 2016
    Assignees: BIOMEDICAL RESEARCH GROUP INC.
    Inventors: Gen-Ichiro Soma, Chie Kohchi, Hiroyuki Inagawa, Takashi Nishizawa, Yukinori Takahashi
  • Patent number: 9388391
    Abstract: The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
    Type: Grant
    Filed: April 22, 2015
    Date of Patent: July 12, 2016
    Assignee: Codexis, Inc.
    Inventors: Michael A. Crowe, Oscar Alvizo, Behnaz Behrouzian, Yong Koy Bong, Steven J. Collier, Anupam Gohel, Jagadeesh Mavinahalli, Naga K. Modukuru, Emily Mundorff, Derek J. Smith, Shiwei Song, Wan Lin Yeo
  • Patent number: 9365878
    Abstract: The present disclosure relates to biocatalysts and its uses for the efficient preparation of eslicarbazepine, eslicarbazepine acetate, and analogs thereof.
    Type: Grant
    Filed: July 2, 2015
    Date of Patent: June 14, 2016
    Assignee: Codexis, Inc.
    Inventors: Anupam Gohel, Derek J. Smith, Brian Wong, Joly Sukumaran, Wan Lin Yeo, Steven J. Collier, Scott Novick
  • Patent number: 9353401
    Abstract: The present invention encompasses modified luciferases, methods for making modified luciferases, and assays utilizing modified luciferases. Modified luciferases of the invention show increased activity over wildtype luciferases and also show increased stability of signal. The present invention also encompasses multiplex assays utilizing multiple luciferases reporters with different emission spectra and different substrates for simultaneous luciferase measurements.
    Type: Grant
    Filed: August 27, 2010
    Date of Patent: May 31, 2016
    Assignee: Targeting Systems
    Inventor: Rampyari Walia
  • Patent number: 9353355
    Abstract: The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
    Type: Grant
    Filed: August 5, 2015
    Date of Patent: May 31, 2016
    Assignee: Codexis, Inc.
    Inventors: Christopher K. Savile, Emily Mundorff, Jeffrey C. Moore, Paul N. Devine, Jacob M. Janey