Oxidoreductase (1. ) (e.g., Luciferase) Patents (Class 435/189)
  • Patent number: 11794145
    Abstract: An atmospheric greenhouse gas reduction system that includes greenhouse gas reduction particles having carbon dioxide conversion material that is capable of converting carbon dioxide in atmosphere into simple carbohydrates. The greenhouse gas reduction particles have a size of up to about 1,000 nanometers.
    Type: Grant
    Filed: September 28, 2021
    Date of Patent: October 24, 2023
    Assignee: Nano BlueSkies Corp
    Inventors: Lee Frelich, John MacDonald
  • Patent number: 11786565
    Abstract: Disclosed herein are compositions and methods for treating periodontal disease.
    Type: Grant
    Filed: March 18, 2020
    Date of Patent: October 17, 2023
    Assignee: Trustees of Tufts College
    Inventors: Linden Hu, Louis Cornacchione
  • Patent number: 11781178
    Abstract: Provided herein are compositions and methods for the synthesis, amplification, and in vitro transcription of full-length cDNA, or cDNA fragments. Methods are provided for reverse transcription of RNA and amplification for in vitro transcription. Further provided are method for loading of dendritic cells with the RNA and homologous lysate for immune stimulation.
    Type: Grant
    Filed: December 17, 2018
    Date of Patent: October 10, 2023
    Assignee: Baylor College of Medicine
    Inventors: William K. Decker, Vanaja Konduri
  • Patent number: 11725220
    Abstract: Microbial enzymes are used for esterification of biomass-derived substrates for production of industrially valuable esters. E. coli was used as an efficient platform for recombinant synthesis of fusel lactates such as the green solvent ethyl lactate.
    Type: Grant
    Filed: August 26, 2020
    Date of Patent: August 15, 2023
    Assignees: National Technology & Engineering Solutions of Sandia, LLC, Arizona Board of Regents on Behalf of Arizona State University
    Inventors: Ryan Wesley Davis, Arul Mohzy Varman, Aditya Pandharinath Sarnaik, Amit Kumar Jha
  • Patent number: 11697842
    Abstract: Provided herein are compositions and methods for the synthesis, amplification, and in vitro transcription of full-length cDNA, or cDNA fragments. Methods are provided for reverse transcription of RNA and amplification for in vitro transcription. Further provided are method for loading of dendritic cells with the RNA and homologous lysate for immune stimulation.
    Type: Grant
    Filed: December 17, 2018
    Date of Patent: July 11, 2023
    Assignee: Baylor College of Medicine
    Inventors: William K. Decker, Vanaja Konduri
  • Patent number: 11685944
    Abstract: A culture system for culturing a microaerophilic or an anaerobic microorganism is provided. The culture system can include effective amounts of i) an enzyme of an oxidoreductase family and ii) a substrate for said enzyme, a container, and a predetermined volume of aqueous medium that supports growth of said anaerobic or microaerophilic microorganism. The enzyme can be selected from a group consisting of ascorbic acid oxidase and laccase. The effective amounts are effective to deplete dissolved oxygen in the predetermined volume to a concentration that facilitates growth of a microaerophilic microorganism or an obligately-anaerobic microorganism. A method of using the system is also provided.
    Type: Grant
    Filed: May 13, 2015
    Date of Patent: June 27, 2023
    Assignee: NEOGEN FOOD SAFETY US HOLDCO CORPORATION
    Inventors: Jason W. Bjork, Adam J. Stanenas, Wensheng Xia
  • Patent number: 11674161
    Abstract: The present invention relates to novel monooxygenases which are useful in the hydroxylation of aromatic hydrocarbons. They are particularly useful for the production of 1-naththol and 7-hydroxycoumarin from naphthol and 7-Ethoxycoumarin, respectively.
    Type: Grant
    Filed: June 18, 2020
    Date of Patent: June 13, 2023
    Assignee: Covestro Intellectual Property GmbH & Co. KG
    Inventors: Ruijing Guo, Jen-Chieh Lin, Sha Tao, Ying Qian, Chenggang Qiu, Kequan Chen, Kang Li
  • Patent number: 11655462
    Abstract: Disclosed are a mutant microorganism for producing succinic acid exhibiting improved activity of conversion of oxaloacetate to malate through the introduction of genes encoding a malate dehydrogenase, wherein an amino acid residue that interacts with a pyrophosphate moiety of NADH through an amide functional group of a main chain of malate dehydrogenase is glutamine (Gln), and a method of producing succinic acid using the same. The mutant microorganism producing succinic acid according to the present invention is capable of producing a high concentration of succinic acid at the highest productivity compared to other mutant microorganisms reported to date when the microorganism is cultured in a limited medium. In addition, the mutant microorganism is capable of producing succinic acid at higher productivity and product concentration through further advanced fermentation technology.
    Type: Grant
    Filed: May 2, 2019
    Date of Patent: May 23, 2023
    Assignee: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Sang Yup Lee, Kyung-Jin Kim, Jung Ho Ahn, Hogyun Seo, Jong An Lee
  • Patent number: 11629340
    Abstract: The present invention is related to compositions and methods for the regulated and controlled expression of proteins.
    Type: Grant
    Filed: September 2, 2019
    Date of Patent: April 18, 2023
    Assignee: OBSIDIAN THERAPEUTICS, INC.
    Inventors: Vipin Suri, Dan Jun Li, Dexue Sun, Byron Delabarre, Vijaya Balakrishnan, Brian Dolinski, Mara Christine Inniss, Grace Y. Olinger
  • Patent number: 11618890
    Abstract: Provided are non-natural or variant beta-ketoacyl-acyl carrier protein (ACP) synthase (KAS) II enzymes (KASII), polynucleotides encoding such variant KASII, host cells expressing such variant KASII, oils and oil products produced by such cells, and methods of making and using such variant KASII.
    Type: Grant
    Filed: August 21, 2019
    Date of Patent: April 4, 2023
    Assignee: Corbion Biotech, Inc.
    Inventors: Nien-Hsi Ko, Joshua Ferreira, Janice Lau Wee, William E. Maffe, Douglas A. Hattendorf
  • Patent number: 11618908
    Abstract: The present disclosure provides methods and compositions for producing rotundone. In various aspects, the present disclosure provides enzymes, polynucleotides encoding said enzymes, and recombinant microbial host cells (or microbial host strains) for the production of rotundone. In some embodiments, the present disclosure provides microbial host cells for producing rotundone at high purity and/or yield, from either enzymatic transformation of ?-guaiene, or from sugar or other carbon source. The present disclosure further provides methods of making products containing rotundone, including flavor or fragrance products, among others.
    Type: Grant
    Filed: September 6, 2019
    Date of Patent: April 4, 2023
    Assignee: MANUS BIO INC.
    Inventors: Ryan N. Philippe, Ajikumar Parayil Kumaran, Christine Nicole S. Santos, Jason Donald, Stephen Sarria
  • Patent number: 11597916
    Abstract: Described are nicotine-degrading enzyme variants that exhibit increased nicotine-degrading activity and/or decreased immunogenicity relative to the wild-type NicA2 and NOX enzymes, compositions comprising the variants, and methods using them.
    Type: Grant
    Filed: February 2, 2018
    Date of Patent: March 7, 2023
    Assignees: ANTIDOTE THERAPEUTICS, INC., BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: Matthew W. Kalnik, Thomas Thisted, Everett Stone, Charles C. Reed, Max Rodnick-Smith
  • Patent number: 11591578
    Abstract: The present invention provides improved P450-BM3 variants with improved activity. In some embodiments, the P450-BM3 variants exhibit improved activity over a wide range of substrates.
    Type: Grant
    Filed: March 16, 2021
    Date of Patent: February 28, 2023
    Assignee: Codexis, Inc.
    Inventors: Robert Osborne, Vesna Mitchell, Khin Yu Naing Htwe, Xiyun Zhang, Erika M. Milczek, Jeffrey C. Moore
  • Patent number: 11566265
    Abstract: The invention relates to the production of one or more terpenoids through microbial engineering, and relates to the manufacture of products comprising terpenoids.
    Type: Grant
    Filed: May 7, 2021
    Date of Patent: January 31, 2023
    Assignees: Massachusetts Institute of Technology, National University of Singapore
    Inventors: Parayil K. Ajikumar, Gregory Stephanopoulos, Heng Phon Too
  • Patent number: 11535829
    Abstract: Disclosed are genetically engineered organisms, such as yeast and bacteria, that have the ability to metabolize atypical phosphorus or sulfur sources. Fermentation methods using the genetically engineered organisms are also described. The fermentation methods are robust processes for the industrial bioproduction of a variety of compounds, including commodities, fine chemicals, an pharmaceuticals.
    Type: Grant
    Filed: November 27, 2018
    Date of Patent: December 27, 2022
    Assignee: Ginkgo Bioworks, Inc.
    Inventors: Arthur J. Shaw, IV, Colin R. South, Johannes P. Van Dijken
  • Patent number: 11525123
    Abstract: The present disclosure provides cytochrome P450 variants useful for carrying out in vivo and in vitro carbene insertion reactions. Methods for preparing carbene insertion products including cyclopropenes, cyclopropanes, bicyclobutanes, substituted lactones, cyclized compounds, and substituted amines are also described.
    Type: Grant
    Filed: March 12, 2021
    Date of Patent: December 13, 2022
    Assignee: California Institute of Technology
    Inventors: Kai Chen, Noah P. Dunham, Daniel J. Wackelin, Andrew Z. Zhou, Zhen Liu
  • Patent number: 11505596
    Abstract: A mutant cytochrome protein originated from a cytochrome protein having three heme-binding domains, which mutant cytochrome protein lacks the first heme-binding domain and the second heme-binding domain as counted from the N-terminus, is provided. The mutant cytochrome protein may lack a region(s) containing the first and second heme-binding domains.
    Type: Grant
    Filed: January 26, 2021
    Date of Patent: November 22, 2022
    Assignee: ARKRAY, INC.
    Inventors: Koji Sode, Junko Shimazaki, Kazushige Mori, Katsuhiro Kojima
  • Patent number: 11466259
    Abstract: The present invention provides engineered galactose oxidase (GOase) enzymes, polypeptides having GOase activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing GOase enzymes are also provided. The present invention further provides compositions comprising the GOase enzymes and methods of using the engineered GOase enzymes. The present invention finds particular use in the production of pharmaceutical and other compounds.
    Type: Grant
    Filed: July 2, 2019
    Date of Patent: October 11, 2022
    Assignee: Codexis, Inc.
    Inventors: Christopher Michael Micklitsch, Oscar Alvizo, Jovana Nazor, Harvinder Chagger Maniar, Mikayla Jianghongxia Krawczyk, Margie Tabuga Borra-Garske, Nandhitha Subramanian, Anna Fryszkowska, Nicholas M. Marshall, Agustina Rodriguez-Granillo, Deeptak Verma, Dewan Andrews
  • Patent number: 11466053
    Abstract: Provided are a polypeptide and nucleic acid for encoding the polypeptide, a nucleic-acid construct, an expression vector, and a host cell containing the nucleic acid, an antigen-presenting cell presenting the polypeptide on the surface of the cell, and immune effector cell thereof, a pharmaceutical composition containing the polypeptide, a vaccine containing the nucleic acid, the nucleic acid construct, the expression vector, the host cell, the antigen-presenting cell, and the immune effector cell, and an antibody recognizing the polypeptide. Also provided is a therapeutic method using the polypeptide, the nucleic acid, the pharmaceutical composition, the vaccine, and the antibody. Also provided are a diagnosis method and diagnosis apparatus for detecting the described polypeptide. Also provided is an application of the polypeptide in preparing a vaccine, a tumor diagnosis kit, or a pharmaceutical composition, and an application of the polypeptide or the nucleic acid as a test target in tumor diagnosis.
    Type: Grant
    Filed: May 20, 2019
    Date of Patent: October 11, 2022
    Assignee: BGI SHENZHEN
    Inventors: Yunxia Tang, Bo Li, Yong Hou, Shuntao Luo, Ying Huang, Geng Liu, Dongli Li, Xiumei Lin
  • Patent number: 11407792
    Abstract: Engineered protein electron carriers, microorganisms expressing the same, and methods detecting regulated electron flow are described.
    Type: Grant
    Filed: November 9, 2018
    Date of Patent: August 9, 2022
    Assignee: William Marsh Rice University
    Inventors: Jonathan Silberg, Joshua T. Atkinson, Ian J. Campbell, George N. Bennett
  • Patent number: 11384371
    Abstract: The use of a cytochrome P-450 enzyme comprising SEQ ID NO: 3, or a variant enzyme having at least 70% identity thereto and having CYP-450 activity, for the hydroxylation of an organic compound.
    Type: Grant
    Filed: November 15, 2017
    Date of Patent: July 12, 2022
    Assignee: HYPHA DISCOVERY LIMITED
    Inventors: Jonathan Charles Paul Steele, Antonio De Riso, Headley St Edward Williams, Richard Kerry Phipps, Stephen Keith Wrigley, Kinga Linda Nytko, Vincent Poon, Sebastian Schulz, John Maxim Ward
  • Patent number: 11359000
    Abstract: Disclosed herein are fusion proteins comprising: (a) a first polypeptide comprising Interleukin-2 (IL2); and (b) a second polypeptide, fused in frame to the first polypeptide, wherein the second polypeptide comprises an extracellular domain of Interleukin-2 Receptor alpha (IL2R?), wherein IL2 or IL2R? comprises at least one fewer glycosylation site compared to native IL2 or native IL2R?. Methods of production and methods of therapeutic use of the fusion proteins are also disclosed.
    Type: Grant
    Filed: March 27, 2019
    Date of Patent: June 14, 2022
    Assignee: Bristol-Myers Squibb Company
    Inventors: Mary Struthers, Jonathan Harry Davis, Michael Louis Doyle, Priyanka Apurva Madia
  • Patent number: 11326173
    Abstract: The present invention concerns a method of producing and enantiomerically pure alpha-ionone. Further, the invention concerns a nucleic acid that comprises a sequence that encodes a lycopene-epsilon-cyclase (EC), a lycopene-epsilon-cyclase (EC), plasmids, which encode components of the alpha-ionone biosynthesis and a microorganism that contains heterologous nucleotide sequences which encode the enzymes geranylgeranyl-diphosphate-synthase, isopentenyl-diphosphate-isomerase (IPI), phytoene desaturase-dehydrogenase (crtI), phytoene synthase (crtB), lycopene-epsilon-cyclase (EC) and carotenoid-cleavage-dioxygenase (CCD1). Further, the invention concerns a method of producing highly pure epsilon-carotene.
    Type: Grant
    Filed: August 28, 2015
    Date of Patent: May 10, 2022
    Assignee: PHYTOWELT GREENTECHNOLOGIES GMBH
    Inventors: Guido Jach, Sanae Azdouffal, Katrin Schullehner, Peter Welters, Angela Goergen
  • Patent number: 11319551
    Abstract: The invention relates to biotechnology and provides novel recombinant DNA molecules and engineered proteins for conferring tolerance to protoporphyrinogen oxidase-inhibitor herbicides. The invention also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.
    Type: Grant
    Filed: June 25, 2019
    Date of Patent: May 3, 2022
    Assignee: Monsanto Technology LLC
    Inventors: Artem G. Evdokimov, Clayton T. Larue, Farhad Moshiri, Joel E. Ream, Xuefeng Zhou
  • Patent number: 11312754
    Abstract: A protein hydrolysate composition is provided. The protein hydrolysate composition comprises isolated peptides useful for ameliorating a condition of a subject. Compositions comprising the protein hydrolysate composition are also provided. The compositions may be adapted for oral or topical administration to the subject, and may compose a foodstuff or beverage (e.g. a medical food), a topical composition (e.g. a lotion, cream, etc.), a kit, and the like. A methods of ameliorating the condition of the subject is further provided. The method include administering the protein hydrolysate composition to the subject. The method may be useful in reducing blood pressure, inflammation, reduced immunity, and/or oxidative stress in the subject.
    Type: Grant
    Filed: May 27, 2020
    Date of Patent: April 26, 2022
    Assignee: ACCESS BUSINESS GROUP INTERNATIONAL LLC
    Inventors: Rodney A. Velliquette, Eugene Maly
  • Patent number: 11306295
    Abstract: A new thermostable luciferase of the following mutant luciferase (a) or (b): (a) a mutant of a wild-type luciferase comprising the amino acid sequence of SEQ ID NO: 1, wherein phenylalanine at position 292 and/or phenylalanine at position 294 in the amino acid sequence of SEQ ID NO: 1 is substituted with another amino acid; or (b) a mutant of a luciferase having 93% or more homology with the amino acid sequence of SEQ ID NO: 1, wherein in the amino acid sequence of the mutant, the amino acid at a site corresponding to position 292 and/or position 294 in the amino acid sequence of SEQ ID NO: 1 is substituted with another amino acid.
    Type: Grant
    Filed: March 14, 2018
    Date of Patent: April 19, 2022
    Assignees: TOYO B-NET CO., LTD., TOYO INK SC HOLDINGS CO., LTD.
    Inventors: Ayumi Hori, Yutaka Yamagishi
  • Patent number: 11284577
    Abstract: Stevia varieties with a high content of RebM, are disclosed Further provided are methods for producing Stevia plants having a high RebM content by negatively regulating certain genes selecting the resulting plants, and breeding with such plants to confer such desirable Reb M phenotypes to plant progeny.
    Type: Grant
    Filed: March 7, 2018
    Date of Patent: March 29, 2022
    Assignees: PureCircle USA Inc., KeyGene N.V., The Coca-Cola Company
    Inventors: Avetik Markosyan, Seong Siang Ong, Runchun Jing, Tengfang Huang, Stephen Ezra Schauer, Fayaz Khazi, Indra Prakash, Alec Hayes
  • Patent number: 11284623
    Abstract: The present invention relates to pesticidal mixtures comprising one biological compound and at least one fungicidal, insecticidal or plant growth regulating compound as defined herein and respective agricultural uses thereof.
    Type: Grant
    Filed: June 8, 2018
    Date of Patent: March 29, 2022
    Assignee: BASF CORPORATION
    Inventors: Lutz Brahm, Burghard Liebmann, Ronald Wilhelm, Markus Gewehr
  • Patent number: 11274287
    Abstract: The present invention provides engineered polypeptides that are useful for the asymmetric synthesis of ?-hydroxy-?-amino acids under industrial-relevant conditions. The present disclosure also provides polynucleotides encoding engineered polypeptides, host cells capable of expressing engineered polypeptides, and methods of producing ?-hydroxy-?-amino acids using engineered polypeptides. Compared to other processes of preparation, the use of the engineered polypeptides of the present invention for the preparation of ?-hydroxy-?-amino acids results in high purity of the desired stereoisomers, mild reaction conditions, low pollution and low energy consumption. So, it has good industrial application prospects.
    Type: Grant
    Filed: May 9, 2018
    Date of Patent: March 15, 2022
    Assignee: Enzymaster (Ningbo) Bio-Engineering Co., Ltd
    Inventors: Haibin Chen, Yong Koy Bong, Baoqin Cai, Qing Xu, Tianran Shen, Ameng Zhou, Jiadong Yang, Zhuhong Yang, Yaoyao Ji, Yingxin Zhang
  • Patent number: 11261478
    Abstract: Provided herein are inhibitor-resistant luciferase mutants, and methods of use thereof. In particular, luciferase mutants are provided that are thermal stable and exhibit improved stability in the presence of luciferin break-down products, such as dehydroluciferin. Further provided are assay systems comprising inhibitor-resistant luciferase mutants and amino acid sequences of the inhibitor-resistant luciferase mutants.
    Type: Grant
    Filed: October 13, 2017
    Date of Patent: March 1, 2022
    Assignee: Promega Corporation
    Inventors: Ce Shi, Thomas Kirkland, Poncho Meisenheimer, Lance P. Encell, Mary Hall
  • Patent number: 11236308
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
    Type: Grant
    Filed: May 14, 2020
    Date of Patent: February 1, 2022
    Assignee: Codexis, Inc.
    Inventors: Oscar Alvizo, Nicholas J. Agard, Xinkai Xie, David Entwistle, Birgit Kosjek
  • Patent number: 11236353
    Abstract: The invention relates to biotechnology and provides novel recombinant DNA molecules and engineered proteins for conferring tolerance to protoporphyrinogen oxidase-inhibitor herbicides. The invention also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.
    Type: Grant
    Filed: June 25, 2019
    Date of Patent: February 1, 2022
    Assignee: Monsanto Technology LLC
    Inventors: Artem G. Evdokimov, Clayton T. Larue, Farhad Moshiri, Joel E. Ream, Xuefeng Zhou
  • Patent number: 11203774
    Abstract: The present disclosure relates to the biosynthesis of indigoid dye precursors and their conversion to indigoid dyes. Specifically, the present disclosure relates to methods of using polypeptides to produce indigoid dye precursors from indole feed compounds, and the use of the indigoid dye precursors to produce indigoid dyes.
    Type: Grant
    Filed: April 7, 2020
    Date of Patent: December 21, 2021
    Assignee: The Regents of the University of California
    Inventors: John Eugene Dueber, Zachary Nicholas Russ, Tammy Melody Hsu, Terry Don Johnson, Jr., Bernardo Cervantes, Ramya Lakshmi Prathuri, Shyam Pravin Bhakta, Arthur Muir Fong, III, Luke Nathaniel Latimer
  • Patent number: 11198886
    Abstract: The invention relates to biotechnology and provides novel recombinant DNA molecules and engineered proteins for conferring tolerance to protoporphyrinogen oxidase-inhibitor herbicides. The invention also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.
    Type: Grant
    Filed: June 25, 2019
    Date of Patent: December 14, 2021
    Assignee: Monsanto Technology LLC
    Inventors: Artem G. Evdokimov, Clayton T. Larue, Farhad Moshiri, Joel E. Ream, Xuefeng Zhou
  • Patent number: 11191816
    Abstract: Provided herein are compositions comprising mutant GADPH. Methods for treating or preventing cancer in a subject by administering to the subject a therapeutically effective amount of mutant GAPDH compositions are provided.
    Type: Grant
    Filed: January 19, 2017
    Date of Patent: December 7, 2021
    Assignee: The Johns Hopkins University
    Inventor: Shanmugasundaram Ganapathy-Kanniappan
  • Patent number: 11180017
    Abstract: A device for driving a vehicle including an engine that serves as a power source of the vehicle, and a transmission that is connected to the engine, the engine and the transmission being arranged transversely such that an axial direction of an output shaft of the engine accords with a right-left direction of the vehicle includes a motor generator (MG) that serves as a power source of the vehicle, and a speed reducer that is connected to the MG. The MG and at least a part of the speed reducer are arranged outside of an engine compartment that accommodates the engine and the transmission. An output shaft of the speed reducer is connected to a power transmission system, which transmits power of an output shaft of the transmission to a drive shaft of a vehicle wheel to be capable of transmitting its power to the power transmission system.
    Type: Grant
    Filed: July 22, 2016
    Date of Patent: November 23, 2021
    Assignee: DENSO CORPORATION
    Inventors: Tomoyuki Shinkai, Takashi Sato, Takurou Nakaoka, Takeo Maekawa, Keiji Kondou
  • Patent number: 11162081
    Abstract: A ketoreductase mutant and use thereof are provided. The amino acid sequence of the ketoreductase mutant is an amino acid sequence obtained by mutation of the amino acid sequence shown in SEQ ID NO: 1, wherein the mutation at least comprises one of the following mutation sites: position 6, position 94, position 96, position 117, position 144, position 156, position 193, position 205, position 224, position 176, position 85 and position 108; alternatively, the amino acid sequence of the ketoreductase mutant has a mutation site in a mutated amino acid sequence and an amino acid sequence having 80% or more homology with the mutated amino acid sequence.
    Type: Grant
    Filed: January 22, 2018
    Date of Patent: November 2, 2021
    Assignee: JILIN ASYMCHEM LABORATORIES CO., LTD.
    Inventors: Hao Hong, Gage James, Jiangping Lu, Xingfu Xu, Wenyan Yu, Na Zhang, Yulei Ma, Yibing Cheng, Huiyan Mu
  • Patent number: 11136608
    Abstract: This Invention discloses a method for production of N-Acetyl-D-Glucosamine and/or D-Glucosamine Salt by microbial fermentation. The method is intended to manufacture N-Acetyl-D-Glucosamine and/or D-Glucosamine Salt in higher efficiency and higher yield, by expression of vitreoscilla hemoglobin in microorganism.
    Type: Grant
    Filed: April 14, 2017
    Date of Patent: October 5, 2021
    Inventor: Lan Sun
  • Patent number: 11124845
    Abstract: The presently disclosed subject matter provides methods, reporter gene constructs, and kits for using prostate-specific membrane antigen (PSMA) as an imaging reporter to image a variety of cells and tissues.
    Type: Grant
    Filed: March 18, 2015
    Date of Patent: September 21, 2021
    Assignee: The Johns Hopkins university
    Inventors: Martin G. Pomper, Mark Castanares, Il Minn, Shawn Lupold
  • Patent number: 11118200
    Abstract: The present invention relates to the preparation of amines from aldehydes and ketones by reductive amination with enzymes having a reductive aminase activity on aldehydes and ketones devoid of any carboxyl group gamma of the carbonyl group. The invention also relates to the enzymes per se and their uses in biocatalysis. The enzymes are derived from Mycobacterium smegmatis and vaccae, Cystobacter fuscus, Microbacterium sp. and Aminomonas paucivorans.
    Type: Grant
    Filed: July 5, 2018
    Date of Patent: September 14, 2021
    Assignees: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES, UNIVERSITE D'EVRY VAL D'ESSONNE (UEVE)
    Inventors: Carine Vergne-Vaxelaire, VĂ©ronique De Berardinis, Anne Zaparucha
  • Patent number: 11078505
    Abstract: The present disclosure relates to polypeptides having transaminase activity, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
    Type: Grant
    Filed: March 3, 2020
    Date of Patent: August 3, 2021
    Assignee: Codexis, Inc.
    Inventors: Christopher K. Savile, Emily Mundorff, Jeffrey C. Moore, Paul N. Devine, Jacob M. Janey
  • Patent number: 11008582
    Abstract: The invention relates to a method for increasing the yield and biomass of a plant, by means of an increase in the expression of the L-aspartate oxidase in the plant. The method according to the invention allows an increase in the photosynthetic capacities of the plants as a result of an increase in the quantities of NAD and the derivatives thereof in said plants. The invention relates to the plants produced by such a method.
    Type: Grant
    Filed: February 24, 2015
    Date of Patent: May 18, 2021
    Assignees: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS), UNIVERSITE PARIS-SUB
    Inventors: Linda De Bont, Bertrand Gakiere
  • Patent number: 10995356
    Abstract: The present disclosure provides a culture device for enumerating colonies of microorganisms. The device can comprise a base, a coversheet, and a nonporous spacer member disposed therebetween. The spacer member comprises an aperture that defines a growth compartment. Disposed in the growth compartment are a cold water-soluble gelling agent, a dry oxygen-scavenging reagent, a dry buffer system, and an effective amount of a dry carbon dioxide-generating reagent. The buffer system is selected such that, when the growth compartment is hydrated with a predetermined volume of deionized water, an aqueous mixture with a pH less than or equal to 6.35 is formed.
    Type: Grant
    Filed: April 26, 2016
    Date of Patent: May 4, 2021
    Assignee: 3M INNOVATIVE PROPERTIES COMPANY
    Inventors: Jason W. Bjork, Evan D. Brutinel, Adam J. Stanenas
  • Patent number: 10995320
    Abstract: The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.
    Type: Grant
    Filed: December 12, 2019
    Date of Patent: May 4, 2021
    Assignee: Codexis, Inc.
    Inventors: Michael A. Crowe, Oscar Alvizo, Behnaz Behrouzian, Yong Koy Bong, Steven J. Collier, Anupam Gohel, Jagadeesh Mavinahalli, Naga K. Modukuru, Emily Mundorff, Derek J. Smith, Shiwei Song, Wan Lin Yeo
  • Patent number: 10975403
    Abstract: The present invention relates to the production of eriodictyol via bioconversion.
    Type: Grant
    Filed: July 11, 2018
    Date of Patent: April 13, 2021
    Assignee: Conagen Inc.
    Inventors: Rui Zhou, Xiaodan Yu, Steven Chen
  • Patent number: 10975243
    Abstract: A genetically modified microorganism includes: an exogenous nucleic acid sequence encoding naphthalene dioxygenase (NDO), wherein the endogenous icd gene of the genetically modified microorganism is knocked out, in which the endogenous icd gene encodes isocitrate dehydrogenase (IDH), and wherein the genetically modified microorganism is capable of using glutamic acid and/or a salt thereof as a nitrogen source to grow and producing indigo dye.
    Type: Grant
    Filed: December 28, 2018
    Date of Patent: April 13, 2021
    Assignee: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE
    Inventors: Pei-Ching Chang, Jhong-De Lin, Chang-Jung Chang, Ya-Lin Lin, Hsiang-Yuan Chu, Jie-Len Huang
  • Patent number: 10961560
    Abstract: A mutated tryptophan oxidase suitable for practical implementation is described herein. Specifically, a mutated tryptophan oxidase wherein at least one amino acid residue of a wild-type tryptophan oxidase is mutated and, as a result, has higher tryptophan oxidase activity and/or stability as compared to the wild-type tryptophan oxidase. The mutated tryptophan oxidase can be derived from a wild-type tryptophan oxidase having at least one of Motifs (2), (3), (5), (7), (9), (11), (13), and (14), and at least one amino acid residue in any of these motifs can have mutation. The mutated tryptophan oxidase also can have a mutation of one or more amino acid residues in an amino acid sequence represented by SEQ ID NO: 2 and a sequence homologous thereto.
    Type: Grant
    Filed: March 6, 2019
    Date of Patent: March 30, 2021
    Assignee: AJINOMOTO CO., INC.
    Inventors: Hiroki Yamaguchi, Kazutoshi Takahashi, Moemi Tatsumi
  • Patent number: 10851141
    Abstract: The present invention provides a mutant beetle luciferase and the like, having mutation in which the amino acid corresponding to valine at position 288 in the amino acid sequence of wild-type Photinus pyralis luciferase is isoleucine, leucine or phenylalanine, mutation in which the amino acid corresponding to leucine at position 376 in the aforementioned sequence is proline, mutation in which the amino acid corresponding to glutamic acid at position 455 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, or mutation in which the amino acid corresponding to glutamic acid at position 488 in the aforementioned sequence is valine, alanine, serine, leucine, isoleucine or phenylalanine, in the amino acid sequence encoding a wild-type beetle luciferase, and characterized in that a luminescence intensity due to a luciferin-luciferase luminescence reaction in a 0.9% by mass NaCl solution is 50% or more of that in a NaCl-free solution.
    Type: Grant
    Filed: June 19, 2017
    Date of Patent: December 1, 2020
    Assignee: DKK-TOA Corporation
    Inventors: Kenichi Noda, Satoshi Yawata, Ai Shimomura
  • Patent number: 10851351
    Abstract: The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.
    Type: Grant
    Filed: February 25, 2019
    Date of Patent: December 1, 2020
    Assignee: Codexis, Inc.
    Inventors: Fabien Louis Cabirol, Haibin Chen, Anupam Gohel, Steven J. Collier, Derek J. Smith, Birgit Kosjek, Jacob Janey
  • Patent number: 10851397
    Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize chiral compounds.
    Type: Grant
    Filed: June 28, 2019
    Date of Patent: December 1, 2020
    Assignee: Codexis, Inc.
    Inventors: Jack S Liang, Stephan Jenne, Emily Mundorff, Rama Voladri, James J. Lalonde, Gjalt W. Huisman