Carboxylic Ester Hydrolase (3.1.1) Patents (Class 435/197)
  • Patent number: 10669306
    Abstract: Solid supports for use in solid-phase peptide synthesis (SPPS) are provided. The solid supports may include a resin and a protected linker coupled to the resin. The linker may be an N-mercaptoethoxyglycine, an N-mercaptopropoxyglycine, an N-mercaptobutoxyglycine, and/or another suitable linker. Kits for use in SPPS are also provided. The kits may include a solid support, a solution including a thiol or a selenol, one or more pluralities of protected amino acids, and/or a wash buffer. Methods of SPPS are also provided. The methods may include providing a solid support including a resin coupled to a protected linker.
    Type: Grant
    Filed: February 3, 2017
    Date of Patent: June 2, 2020
    Assignee: University of Washington
    Inventors: Champak Chatterjee, Patrick M. Shelton, Caroline E. Weller
  • Patent number: 10590401
    Abstract: The present invention relates to novel esterase, more particularly to esterase variants having improved activity compared to the esterase of SEQ ID NO: 1 and the uses thereof for degrading polyester containing material, such as plastic products. The esterases of the invention are particularly suited to degrade polyethylene terephthalate, and material containing polyethylene terephthalate.
    Type: Grant
    Filed: July 12, 2017
    Date of Patent: March 17, 2020
    Assignee: CARBIOS
    Inventors: Vincent Tournier, Helene Texier, Marie-Laure Desrousseaux, Christopher Topham, Isabelle Andre, Sophie Barbe, Sophie Duquesne, Alain Marty
  • Patent number: 10294463
    Abstract: A modified Bacillus cereus phospholipase C enzyme is provided, as well as a method of using the modified phospholipase C enzyme in a method of treating vegetable oil. In certain embodiments, this method may comprise combining a vegetable oil with an modified phospholipase C enzyme comprising an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO:1, wherein the amino acid residue at position 66 is a Trp (W) or Tyr (Y), and maintaining the combination under conditions suitable for the modified phospholipase C enzyme to catalyze the hydrolysis of phospholipids in the oil to produce diacylglycerol and a water soluble phosphate.
    Type: Grant
    Filed: June 19, 2014
    Date of Patent: May 21, 2019
    Inventors: Maria Eugenia Castelli, Hugo Menzella, Salvador Peiru, Leandro Vetcher
  • Patent number: 10023898
    Abstract: A method of detecting Listeria monocytogenes. The method comprises providing a culture device with a selective culture medium and a detection article comprising a first indicator system. The selective culture medium facilitates the growth of Listeria microorganisms. When a Listeria microorganism is detected in a sample contacted with the culture medium, the detection article is contacted with the culture medium to detect Listeria monocytogenes.
    Type: Grant
    Filed: June 21, 2017
    Date of Patent: July 17, 2018
    Assignee: 3M Innovative Properties Company
    Inventors: Takatoshi Moriyama, Patrick A. Mach, Akio Kitahara, Henry J. Lubrant
  • Patent number: 9988652
    Abstract: The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterologous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability of Kluveryomyces to produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.
    Type: Grant
    Filed: July 1, 2015
    Date of Patent: June 5, 2018
    Assignees: Lallemand Hungary Liquidity Management LLC, Stellenbosch University
    Inventors: John McBride, Elena Brevnova, Mark Mellon, Allan Froehlich, Kristen Deleault, Vineet Rajgarhia, Riaan Den Haan, Merja Penttila, Marja Ilmen, Matti Siika-Aho, Jaana Uusitalo, Emily A. Stonehouse, Alan Gilbert, Haowen Xu, Deidre Willes, John Bardsley, Anu Koivula, Sanni Voutilainen
  • Patent number: 9719124
    Abstract: A method of detecting Listeria monocytogenes. The method comprises providing a culture device with a selective culture medium and a detection article comprising a first indicator system. The selective culture medium facilitates the growth of Listeria microorganisms. When a Listeria microorganism is detected in a sample contacted with the culture medium, the detection article is contacted with the culture medium to detect Listeria monocytogenes.
    Type: Grant
    Filed: December 2, 2015
    Date of Patent: August 1, 2017
    Assignee: 3M INNOVATIVE PROPERTIES COMPANY
    Inventors: Takatoshi Moriyama, Patrick A. Mach, Akio Kitahara, Henry J. Lubrant
  • Patent number: 9695406
    Abstract: The present invention relates to glucoamylase variants having increased thermostability. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: March 6, 2014
    Date of Patent: July 4, 2017
    Assignee: NOVOZYMES A/S
    Inventors: Esben P. Friis, Jesper Vind, Leonardo De Maria, Bjarne G. Hansen
  • Patent number: 9493748
    Abstract: The present invention provides methods and uses for treating pyropheophytin-containing compositions, particularly in order to remove pyropheophytin therefrom. The compositions are typically plant, algal, or bacterial derived products such as vegetable oils. The method comprises a step of contacting the compositions with an enzyme having pyropheophytinase activity. Also provided are related apparatus and products for performing such methods and uses.
    Type: Grant
    Filed: June 10, 2010
    Date of Patent: November 15, 2016
    Assignee: DUPONT NUTRITION BIOSCIENCES APS
    Inventors: Jørn Borch Søe, Charlotte Horsmans Poulsen, Masoud Rajabi Zargahi, Jens Frisbaæk Sørensen, Tina Jørgensen, Janne Brunstedt, Rene Mikkelsen, Susan Mampusti Madrid
  • Patent number: 9476072
    Abstract: The present invention relates to cutinase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: April 30, 2013
    Date of Patent: October 25, 2016
    Assignee: Novozymes A/S
    Inventors: Aditya Basu, Naik Sangeeta, Santhosh Mepadam Vasu, Paul Pritish, Rakhi Saikia, Allan Svendsen
  • Patent number: 9416354
    Abstract: The cDNA and amino acid sequences of a ferulate esterase obtained from Lactobacillus fermentum NRRL B-1932 is determined. An expression vector for expression of the ferulate esterase gene is generated. The recombinate ferulate esterase gene and transcribed protein contains a linker sequence and 6×HIS tag for purification. Enzymatic activity of the recombinant protein is determined.
    Type: Grant
    Filed: February 24, 2015
    Date of Patent: August 16, 2016
    Assignee: The United States of America, as represented by the Secretary of Agriculture
    Inventor: Siqing Liu
  • Patent number: 9388443
    Abstract: The present disclosure relates to an enzyme derived from Candida bombicola that is capable of lactonizing or polymerizing carbohydrate-containing compounds, lipids, fatty acids, hydroxylated fatty acids, alcohols, dicarboxylic acids or mixtures thereof. Hence, host cells comprising the latter enzyme can be used, via the formation of intra- or inter-molecular ester-bounds, to produce, for example, lactonized sophorolipids or polymers of acidic sophorolipids. On the other hand, host cells having lost their capability to produce a functional enzyme disclosed herein can be used to produce 100% acidic sophorolipids.
    Type: Grant
    Filed: December 14, 2012
    Date of Patent: July 12, 2016
    Assignee: Universiteit Gent
    Inventors: Inge Van Bogaert, Katarzyna Ciesielska, Bart Devreese, Wim Soetaert, Sophie Roelants
  • Publication number: 20150147783
    Abstract: The present invention relates to an isolated mutant eubacterium comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product of interest is compared to the production of the same product in an isogenic wild type strain grown at identical conditions, wherein the substitution of at least one amino acid occurs at any of positions 469, 478, 482, 485, or 487 of SEQ ID NO:2, or at the equivalent positions in any eubacterial RNA-polymerase beta-subunit family member. Another aspect of the invention relates to a process for producing at least one product of interest in a mutant eubacterium and to a use of the mutant eubacterium according to the invention for producing at least one product of interest.
    Type: Application
    Filed: February 9, 2015
    Publication date: May 28, 2015
    Inventors: Steen Troels Joergensen, Jens Toenne Andersen, Niels Banke, Preben Nielsen
  • Publication number: 20150139977
    Abstract: The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal ?-1,4-xylosidic linkages or endo-?-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.
    Type: Application
    Filed: October 22, 2014
    Publication date: May 21, 2015
    Inventors: David Weiner, David Blum, Alexander Varvak, Shaun Healey, Kristine Chang, Geoff Hazlewood, Thomas Todaro, Grace Desantis, Hwai Chang, Connie Jo Hansen, Scott W. Beaver, Thomas Woodward, Charles Hancock
  • Publication number: 20150125925
    Abstract: The present invention provides consumer products comprising a serine protease variant, more specifically subtilisin variants produced there from. Specifically, the present invention provides consumer products comprising a serine protease variant, more specifically a subtilisin variant having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides methods of making such compositions and methods of treating surfaces, particularly fabric surfaces comprising contacting a surface with an aqueous liquor comprising a serine protease variant, more specifically subtilisin variants and an adjunct material.
    Type: Application
    Filed: November 5, 2013
    Publication date: May 7, 2015
    Applicant: The Procter & Gamble Company
    Inventors: Philip Frank SOUTER, Euan John MAGENNIS, Glenn Steven WARD, Neelam S. AMIN, Katherine AUGUSTYN, Joshua R. BASLER, Luis Gustavo CASCAO-PEREIRA, Katherine D. COLLIER, Edward M. CONCAR, David A. ESTELL, James T. KELLIS, JR., Alexander PISARCHIK, Ayrookaran Joseph POULOSE, Jian YAO
  • Patent number: 9017982
    Abstract: Provided are non-wild-type organophosphorus acid anhydrolases that are capable of degrading (ethyl {2-[bis(propan-2-yl)amino]ethyl}sulfanyl) (methyl)phosphinate and other V-agents. Particular embodiments include an organophosphorus acid anhydrolase including an amino acid substitution at position 212.
    Type: Grant
    Filed: December 17, 2013
    Date of Patent: April 28, 2015
    Assignee: The United States of America as Represented by the Secretary of the Army
    Inventors: Saumil S. Shah, Mark A. Guelta, Steven P. Harvey
  • Publication number: 20150093790
    Abstract: The disclosure provides thermostable enzymes isolated from Caldicellulosiruptor bescii and fragments thereof useful for the degradation of cellulose and/or hemicellulose, including thermostable cellulases and hemicellulases. The disclosure further provides nucleic acids encoding the thermostable enzymes of the disclosure. The disclosure also provides methods for the conversion of cellulose and hemicellulose into fermentable sugars using thermostable enzymes of the disclosure. The disclosure also provides enzyme cocktails containing multiple enzymes disclosed herein. The enzymes can be used to release sugars present in cellulose or hemicellulose for subsequent fermentation to produce value-added products.
    Type: Application
    Filed: December 20, 2011
    Publication date: April 2, 2015
    Applicant: The Board pf Trustees of the University of Illinois
    Inventors: Yejun Han, Xiaoyun Su, Dylan Dodd, Roderick I. Mackie, Issac K.O. Cann
  • Publication number: 20150093795
    Abstract: The present invention relates tocutinasevariants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Application
    Filed: April 30, 2013
    Publication date: April 2, 2015
    Inventors: Aditya Basu, Naik Sangeeta, Santhosh Mepadam Vasu, Paul Pritish, Rakhi Saikia, Allan Svendsen
  • Patent number: 8962294
    Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
    Type: Grant
    Filed: October 22, 2012
    Date of Patent: February 24, 2015
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Robert Dicosimo, Mark Payne Scott, John Edward Gavagan
  • Patent number: 8956843
    Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
    Type: Grant
    Filed: October 22, 2012
    Date of Patent: February 17, 2015
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Robert DiCosimo, Mark Scott Payne, John Edward Gavagan
  • Patent number: 8956838
    Abstract: The present invention relates to a polynucleotide encoding an enzyme having carboxyl esterase [E.C. 2.1.1.1] activity.
    Type: Grant
    Filed: April 27, 2007
    Date of Patent: February 17, 2015
    Assignee: B.R.A.I.N.
    Inventors: Christian Elend, Karl-Erich Jaeger, Christian Leggewie, Christel Vollstedt, Wolfgang Streit
  • Publication number: 20150044736
    Abstract: Described are compositions and methods relating to a fungal cutinase cloned from Magnaporthe grisea, polynucleotides encoding the cutinase, and methods of use thereof. The compositions and methods have particular application in detergent cleaning compositions and synthesis reactions.
    Type: Application
    Filed: July 23, 2014
    Publication date: February 12, 2015
    Applicant: DANISCO US INC.
    Inventors: Christian D. Adams, Andrei Miasnikov
  • Patent number: 8951767
    Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: July 31, 2012
    Date of Patent: February 10, 2015
    Assignee: Novozymes A/S
    Inventor: Nikolaj Spodsberg
  • Patent number: 8945901
    Abstract: Butyrylcholinesterase (BChE) polypeptide variants of the presently-disclosed subject matter have enhanced catalytic efficiency for (?)-cocaine, as compared to wild-type BChE. Pharmaceutical compositions of the presently-disclosed subject matter include a BChE polypeptide variant having an enhanced catalytic efficiency for (?)-cocaine. A method of the presently-disclosed subject matter for treating a cocaine-induced condition includes administering to an individual an effective amount of a BChE polypeptide variant, as disclosed herein, to lower blood cocaine concentration.
    Type: Grant
    Filed: October 23, 2013
    Date of Patent: February 3, 2015
    Assignee: University of Kentucky Research Foundation
    Inventors: Chang-Guo Zhan, Fang Zheng, Wenchao Yang, Liu Xue, Shurong Hou
  • Publication number: 20150031111
    Abstract: The present invention relates to isolated lipase variants, comprising a substitution at one or more positions corresponding to positions I86D,E,N,Q, E87C, I90D,E,Q, and N92D,E,Q of the mature polypeptide of SEQ ID NO: 2, wherein the variant has lipase activity. In some embodiments the present invention relates to isolated polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; methods of producing the variants; and compositions comprising the variants. The present invention also relates to methods of obtaining lipase variants; methods of cleaning; and use of lipase variants for cleaning.
    Type: Application
    Filed: March 22, 2013
    Publication date: January 29, 2015
    Inventors: Jakob Skjold-Joergensen, Jesper Vind, Allan Svendsen
  • Patent number: 8940876
    Abstract: The present disclosure relates to a method for preparing recombinant glycoproteins with high sialic acid content. More specifically, for UDP-GlcNAc 2-epimerase/ManNAc kinase (GNE/MNK) enzyme where point mutation was induced by substituting arginine at position 263 by leucine only or by further substituting arginine at position 266 by glutamine, epimerase activity is constantly maintained, and overexpressed cells thereof experience an increase in intracellular cytidine monophosphate (CMP)-sialic acid content, irrespective of CMP-sialic acid concentration.
    Type: Grant
    Filed: February 1, 2011
    Date of Patent: January 27, 2015
    Assignee: Korea Advanced Institute of Science and Technology
    Inventors: Jung Hoe Kim, Young Dok Son, Jin Young Hwang, Yeon Tae Jeong
  • Patent number: 8940514
    Abstract: A thioesterase comprising an amino acid sequence set forth in SEQ ID NO: 1, a thioesterase gene encoding the thioesterase, a transformant comprising the gene, and a method of producing fatty acids or lipids using the transformant.
    Type: Grant
    Filed: April 13, 2011
    Date of Patent: January 27, 2015
    Assignee: Kao Corporation
    Inventors: Takuto Tojo, Keiji Endo
  • Publication number: 20150024465
    Abstract: The present invention relates to non-catalytic carbohydrate-binding modules (CBM) belonging to a new family of CBM's. A CBM of the invention was found attached to a glycosyl hydrolase family 61 (GH61) polypeptide and was shown to have little homology with known CBM's indicating that it is the first known member of a new family of CBM's. The present invention further relates to CBM's preferably exhibiting binding affinity for cellulose; to a method of producing such CBM's; and to methods for using such CBM's in the textile, detergent and cellulose fiber processing industries, for purification of polypeptides, immobilisation of active enzymes, baking, manufacturing of biofuel, modification of plant cell walls.
    Type: Application
    Filed: September 29, 2014
    Publication date: January 22, 2015
    Inventors: Kirk Matthew Schnorr, Lars Lehmann Hylling Christensen
  • Patent number: 8932837
    Abstract: Process for producing optically active 3-aminocarboxylic acid ester compounds of general Formula I, and the ammonium salts thereof, in which R1 stands for alkyl, alkoxyalkyl, alkenyl, cycloalkyl, heterocycloalkyl, aryl, or hetaryl, and R2 stands for alkyl, cycloalkyl or aryl, in which an enantiomeric mixture of a simply N-acylated 3-aminocarboxylic acid ester of general formula (I.b), in which R1 and R2 have the meanings given above and R3 stands for hydrogen, alkyl, cycloalkyl or aryl, is submitted to an enantioselective deacylation by adding a polypeptide according to claim 1.
    Type: Grant
    Filed: July 15, 2013
    Date of Patent: January 13, 2015
    Assignee: BASF SE
    Inventors: Bernhard Hauer, Thomas Friedrich, Rainer Stürmer, Nina Schneider, Susanne Sabrowski, Wolf-Rüdiger Krahnert
  • Publication number: 20150004659
    Abstract: The disclosure relates to a nucleic acid molecule isolated from a Papaver somniferum cultivar that produces the opiate alkaloid noscapine which comprises 10 genes involved in the biosynthesis of opiate alkaloids.
    Type: Application
    Filed: March 12, 2013
    Publication date: January 1, 2015
    Applicant: GlaxoSmithKline Australia Pty Limited
    Inventors: Thilo Winzer, Ian Alexander Graham, Tracy Carol Walker
  • Patent number: 8921080
    Abstract: The invention relates to an isolated polypeptide having esterase activity comprising an amino acid sequence shown in any one of SEQ ID NO's 2, 4, 6, 8, 10, 12 or 14 or a homologue thereof, comprising an amino acid substitution or deletion of one or more amino acids as shown in said SEQ ID NO's and resulting in a mutant polypeptide having an increased concentration of the fraction of the mutant polypeptide being present as an active and soluble protein in cleared lysate of the mutant polypeptide expressed in E. coli relative to the concentration of the fraction of the polypeptide without the mutation being present as an active and soluble protein in cleared lysate of the polypeptide without the one or more deletion or substitution expressed in E. coli under the same conditions. The invention also relates to nucleic acid encoding the polypeptides according to the invention, and the use of the polypeptides.
    Type: Grant
    Filed: April 26, 2010
    Date of Patent: December 30, 2014
    Assignee: DSM IP Assets B.V.
    Inventors: Martin Kietzmann, Harald Pichler, Helmut Schwab, Amin El-Heliebi, Christine Winkler, Andreas Braun
  • Patent number: 8911977
    Abstract: Acetyl xylan esterases and variants thereof having perhydrolytic activity are provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. Multi-component peracid generation systems comprising an enzyme catalyst having perhydrolytic activity are also provided, as are methods of using the present enzyme catalyst to produce peroxycarboxylic acids. The polypeptide having perhydrolytic activity may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, paper pulp processing, and personal care applications.
    Type: Grant
    Filed: March 13, 2013
    Date of Patent: December 16, 2014
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Mark Scott Payne, Robert DiCosimo
  • Publication number: 20140363877
    Abstract: In one aspect, there is provided a process for treating a plant oil, comprising a step of contacting the oil with an enzyme, wherein the enzyme is capable of hydrolysing an a? or b? stereoisomer of chlorophyll or a chlorophyll derivative.
    Type: Application
    Filed: February 16, 2012
    Publication date: December 11, 2014
    Applicant: DUPONT NUTRITION BIOSCIENCES, APS
    Inventors: Jorn Borch Soe, Tina Lillan Jorgensen, Lene Lauridsen, Rene Mikkelsen, Janne Brunstedt
  • Patent number: 8900830
    Abstract: A process for efficiently producing optically active succinimide derivatives as key intermediates of (3R)-2?-(4-bromo-2-fluorobenzyl)spiro{pyrrolidine-3,4?(1?H)-pyrrolo[1,2-a]pyrazine}-1?,2,3?,5(2?H)-tetraone, which comprises the following reaction steps, and the step 2 is performed by using a non-animal-derived enzyme.
    Type: Grant
    Filed: July 28, 2011
    Date of Patent: December 2, 2014
    Assignees: Sumitomo Dainippon Pharma Co., Ltd., Kyowa Hakko Bio Co., Ltd.
    Inventors: Shinji Kita, Kazuya Tsuzaki, Eitora Yamamura
  • Publication number: 20140335567
    Abstract: The present disclosure relates to an enzyme derived from Candida bombicola that is capable of lactonizing or polymerizing carbohydrate-containing compounds, lipids, fatty acids, hydroxylated fatty acids, alcohols, dicarboxylic acids or mixtures thereof. Hence, host cells comprising the latter enzyme can be used, via the formation of intra- or inter-molecular ester-bounds, to produce, for example, lactonized sophorolipids or polymers of acidic sophorolipids. On the other hand, host cells having lost their capability to produce a functional enzyme disclosed herein can be used to produce 100% acidic sophorolipids.
    Type: Application
    Filed: December 14, 2012
    Publication date: November 13, 2014
    Inventors: Inge Van Bogaert, Katarzyna Ciesielska, Bart Devreese, Wim Soetaert, Sophie Roelants
  • Patent number: 8883485
    Abstract: Described are compositions and methods for the decolorization of dye molecules in an aqueous medium using enzymatically-generated peracid.
    Type: Grant
    Filed: March 2, 2010
    Date of Patent: November 11, 2014
    Assignee: Danisco US Inc.
    Inventors: Christopher C. Barnett, Rafael F. Sala
  • Patent number: 8883444
    Abstract: The invention relates to a method for synthesizing a peptide by enzymatically preparing an ester or thioester from (i) an N-terminal protected amino acid or an N-terminal protected peptide where either can have a protected C-terminal ester group and (ii) an alcohol represented by the formula HO—CX2—Z or a thiol represented by the formula HS—CX2—Z, each X independently representing a halogen atom or a hydrogen atom; and Z represents an electron withdrawing group comprising at least one sp3-hybridized carbon comprising at least two substituents comprising a heteroatom directly attached to the at least one sp3-hybridized carbon or at least one sp2-hybridized carbon comprising one or two substituents comprising a heteroatom directly attached to the at least one sp2-hybridized carbon, and enzymatically coupling the prepared ester or thioester with an optionally C-terminal protected amino acid or with an optionally C-terminal protected peptide in a medium comprising 2 wt. % water or less.
    Type: Grant
    Filed: November 19, 2009
    Date of Patent: November 11, 2014
    Assignee: Enzypep B.V.
    Inventors: Peter Jan Leonard Mario Quaedflieg, Timo Nuijens, Claudia Cusan, Catharina Hubertina Maria Schepers
  • Publication number: 20140329292
    Abstract: This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to a method to release cellular content by degradation of cell walls. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, biorefining, deinking and biobleaching of paper and pulp, and treatment of waste streams.
    Type: Application
    Filed: September 6, 2013
    Publication date: November 6, 2014
    Applicant: DYADIC INTERNATIONAL, INC.
    Inventors: Mark A. EMARLFARB, Alexander Vasilievich Gusakov, Peter J. Punt, Jan Cornelis Verdoes, Arkady Panteleimonovich Sinitsyn, Elena Vlasenko, Sandra Wihelmina Agnes Hinz, Mark Gosink, Zhijie Jiang
  • Patent number: 8865435
    Abstract: Acetyl xylan esterases and variants thereof having perhydrolytic activity are provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. Multi-component peracid generation systems comprising an enzyme catalyst having perhydrolytic activity are also provided, as are methods of using the present enzyme catalyst to produce peroxycarboxylic acids. The polypeptide having perhydrolytic activity may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, paper pulp processing, and personal care applications.
    Type: Grant
    Filed: March 13, 2013
    Date of Patent: October 21, 2014
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Mark Scott Payne, Robert DiCosimo
  • Patent number: 8865437
    Abstract: Acetyl xylan esterases and variants thereof having perhydrolytic activity are provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. Multi-component peracid generation systems comprising an enzyme catalyst having perhydrolytic activity are also provided, as are methods of using the present enzyme catalyst to produce peroxycarboxylic acids. The polypeptide having perhydrolytic activity may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, paper pulp processing, and personal care applications.
    Type: Grant
    Filed: March 13, 2013
    Date of Patent: October 21, 2014
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Mark Scott Payne, Robert DiCosimo
  • Patent number: 8865443
    Abstract: In an alcohol fermentation process, oil derived from biomass is hydrolyzed into an extractant available for in situ removal of a product alcohol such as butanol from a fermentation broth. The glycerides in the oil can be catalytically (e.g., enzymatically) hydrolyzed into free fatty acids, which form a fermentation product extractant having a partition coefficient for a product alcohol greater than a partition coefficient of the oil of the biomass for the product alcohol. Oil derived from a feedstock of an alcohol fermentation process can be hydrolyzed by contacting the feedstock including the oil with one or more enzymes whereby at least a portion of the oil is hydrolyzed into free fatty acids forming a fermentation product extractant, or the oil can be separated from the feedstock prior to the feedstock being fed to a fermentation vessel, and the separated oil can be contacted with the enzymes to form the fermentation product extractant.
    Type: Grant
    Filed: May 15, 2013
    Date of Patent: October 21, 2014
    Assignee: Butamax Advanced Biofuels LLC
    Inventors: Keith H. Burlew, Robert Dicosimo, Michael Charles Grady
  • Patent number: 8865436
    Abstract: Acetyl xylan esterases and variants thereof having perhydrolytic activity are provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. Multi-component peracid generation systems comprising an enzyme catalyst having perhydrolytic activity are also provided, as are methods of using the present enzyme catalyst to produce peroxycarboxylic acids. The polypeptide having perhydrolytic activity may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, paper pulp processing, and personal care applications.
    Type: Grant
    Filed: March 13, 2013
    Date of Patent: October 21, 2014
    Assignee: E. I. du Pont de Nemours and Company
    Inventors: Mark Scott Payne, Robert DiCosimo
  • Patent number: 8859221
    Abstract: An object to be attained by the present invention is to provide a method for conveniently, rapidly, and specifically measuring HDL-C in a specimen by use of inexpensive materials, and to provide a reagent kit for HDL-C detection and a dry analytical element for HDL-C detection. The present invention provides a method for measuring high density lipoprotein cholesterol (HDL-C) in a body fluid test sample, wherein cholesterol esterase derived from Schizophyllum commune or Pseudomonas sp. and cholesterol oxidase derived from Pseudomonas sp. are used to generate hydrogen peroxide from HDL-C, and thereby HDL-C is selectively measured.
    Type: Grant
    Filed: May 10, 2007
    Date of Patent: October 14, 2014
    Assignee: FUJIFILM Corporation
    Inventors: Yoshihiko Abe, Nobuhito Masuda, Hiroko Inomata
  • Publication number: 20140287477
    Abstract: Disclosed are compositions and methods relating to variant maltohexaose-forming alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
    Type: Application
    Filed: October 26, 2012
    Publication date: September 25, 2014
    Applicant: DANISCO US INC.
    Inventors: Luis G. Cascao-Pereira, David A. Estell, Marc Kolkman, Harm Mulder
  • Patent number: 8841098
    Abstract: Acetyl xylan esterases and variants thereof having perhydrolytic activity are provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. Multi-component peracid generation systems comprising an enzyme catalyst having perhydrolytic activity are also provided, as are methods of using the present enzyme catalyst to produce peroxycarboxylic acids. The polypeptide having perhydrolytic activity may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, paper pulp processing, and personal care applications.
    Type: Grant
    Filed: March 13, 2013
    Date of Patent: September 23, 2014
    Assignee: E.I. du Pont de Nemours and Company
    Inventors: Mark Scott Payne, Robert DiCosimo
  • Patent number: 8834865
    Abstract: The invention provides methods, compositions, systems, and kits that include an enzyme/substrate co-delivery system. The liquid delivery system includes at least one enzyme encapsulated in a water-soluble polymeric matrix and a substrate for the enzyme in a carrier liquid in which the polymeric matrix is insoluble. When water is added, the polymeric matrix is solubilized and enzyme is released from the matrix, permitting catalytic action upon the substrate.
    Type: Grant
    Filed: November 3, 2009
    Date of Patent: September 16, 2014
    Assignee: Danisco US Inc.
    Inventors: Nathaniel T. Becker, Michael Stoner, Mee-Young Yoon
  • Publication number: 20140248684
    Abstract: In some aspects, the present invention relates to compositions and methods for the production of human cholinesterases. More particularly, it relates to methods for the production of human cholinesterases using transient expression and vectors for producing the same. In one aspect, the present invention relates to a plant viral vector encoding a plant codon-optimized DNA sequence that results in accumulation of the cholinesterase in a plant leaf at levels greater than 20 mg, and in some embodiments greater than 200 mg, of the enzyme per kilogram of the plant leaf.
    Type: Application
    Filed: September 17, 2012
    Publication date: September 4, 2014
    Applicant: ARIZONA BOARD OF REGENTS FOR AND ON BEHALF OF ARIZONA STATE UNIVERSITY
    Inventors: Tsafrir Mor, Latha Kannan, Katherine E. Larrimore
  • Publication number: 20140248254
    Abstract: This invention provides the use of AChE as nuclease. The use of AChE in regulating the stability of nucleic acid and cell apoptosis, as well as a series of agents based on the use, including the agents for promoting or inhibiting cell apoptosis, the agents for inhibiting tumors, and the agents for protecting nucleic acid from impairing, are provided.
    Type: Application
    Filed: August 8, 2012
    Publication date: September 4, 2014
    Applicant: SHANGHAI INSTITUTES FOR BIOLOGICAL SCIENCES, CHINESE ACADEMY OF SCIENCES
    Inventors: Xuejun Zhang, Jing Xie, Aiying Du, Kaijie Guo, Jun Wu
  • Patent number: 8815550
    Abstract: Disclosed herein are compositions and methods to target enzymatic peracid production to a target surface. The peracid benefit agent produced by the targeted perhydrolytic enzyme can be use for a variety of applications such as bleaching, whitening, disinfecting, destaining, deodorizing, and combinations thereof. Specifically, a fusion protein comprising a perhydrolytic enzyme and at least one peptidic component having affinity for a target surface (excluding body surfaces and oral care surfaces) is used in combination with a suitable substrate and a source of peroxygen to enzymatically produce a peracid on or near the surface of the target material. In a preferred aspect, the target surface is a cellulosic material.
    Type: Grant
    Filed: November 13, 2013
    Date of Patent: August 26, 2014
    Assignee: E I du Pont de Nemours and Company
    Inventors: Robert DiCosimo, Scott D Cunningham, Stephen R Fahnestock, Tanja Maria Gruber, Mark S Payne, Pierre E Rouviere, Linda Jane Solomon, Hong Wang
  • Publication number: 20140234921
    Abstract: The present invention relates to novel polypeptides, or fragments of polypeptides, genes encoding them and means for producing said polypeptides. In detail the invention relates to polypeptides having esterase, suberinase and/or cutinase activity at low pH. This invention relates also to compositions containing the polypeptides and methods of using the polypeptides.
    Type: Application
    Filed: April 11, 2012
    Publication date: August 21, 2014
    Applicant: TEKNOLOGIAN TUTKIMUSKESKUS VTT
    Inventors: Antti Nyyssola, Hanna Kontkanen, Mari Hakkinen, Ville Pihlajaniemi, Markku Saloheimo, Johanna Buchert, Tiina Nakari-Setala
  • Publication number: 20140234915
    Abstract: The present invention relates enzyme composition comprising a cellulolytic preparation and an acetylxylan esterase (AXE); and the used of cellulolytic enzyme compositions for hydrolyzing acetylated cellulosic material. Finally the invention also relates to processes of producing fermentation products from acetylated cellulosic materials using a cellulolytic enzyme composition of the invention.
    Type: Application
    Filed: September 21, 2012
    Publication date: August 21, 2014
    Inventors: Prashant Iyer, Armindo Ribiero Gaspar, James Croonenberghs, Thomas P. Binder