Hydrolase (3. ) Patents (Class 435/195)
  • Patent number: 10329250
    Abstract: According to the present invention, there can be provided a process for producing 7-dehydrocholesterol (7DHC), comprising culturing, in a medium, a 7DHC-producing Labyrinthulea microorganism in which sterol 24-C-methyltransferase activity is reduced or lost as compared to a parent strain, allowing 7DHC to be produced and accumulated in the culture, and collecting the 7DHC from the culture; and a process for producing vitamin D3, comprising irradiating, with ultraviolet light, 7-dehydrocholesterol produced by the production process.
    Type: Grant
    Filed: October 8, 2015
    Date of Patent: June 25, 2019
    Assignee: KYOWA HAKKO BIO CO., LTD.
    Inventors: Tetsuro Ujihara, Satoshi Mitsuhashi
  • Patent number: 10246692
    Abstract: The present invention relates to isolated polypeptides having lipase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
    Type: Grant
    Filed: July 11, 2013
    Date of Patent: April 2, 2019
    Assignee: Novozymes A/S
    Inventors: Robert Piotr Olinski, Preben Nielsen, Kim Borch, Anna Verena Reiser, Carsten Hoerslev Hansen, Lone Baunsgaard
  • Patent number: 10221404
    Abstract: The present invention relates to a method for modifying one or more characteristics of a lipase, comprising the step of associating a peptide to the lipase, wherein the peptide has at least 50% sequence identity with the amino acid sequence of at least one major lipase contact zone of the propeptide of said lipase.
    Type: Grant
    Filed: May 26, 2015
    Date of Patent: March 5, 2019
    Assignee: Novozymes A/S
    Inventors: Anna Verena Reiser, Vikram Kjoeller Bhatia, Kim Borch, Christian I. Joergensen, Birgitte Andersen, Lone Baunsgaard
  • Patent number: 10168323
    Abstract: The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture dimers), surfaces (e.g., displaying, capture ligands, capture proteins, or capture dimers), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.
    Type: Grant
    Filed: March 11, 2014
    Date of Patent: January 1, 2019
    Assignee: Promega Corporation
    Inventors: Carolyn W. Hitko, Robin Hurst, Thomas Kirkland, Dieter Klaubert, Poncho Meisenheimer, Nidhi Nath, Rachel Friedman Ohana, Paul Otto, Marjeta Urh, Harry Tetsuo Uyeda, Keith Wood
  • Patent number: 10047042
    Abstract: The object of the present invention is to provide a method of efficiently manufacturing an optically active D- and/or L-form amino acid possessing a thiol group in the side chain by a simple method. The present invention provides a method of manufacturing an amino acid derivative possessing a thiol group in the side chain, characterized in manufacturing an intermediate composition comprising D- and L-forms of an amino acid derivative possessing a thiol group at the ?-position, reacting a hydrolase selective for D- or L-amino acids, and separating the hydrolyzed D- or L-amino acid derivative, as well as an intermediate thereof.
    Type: Grant
    Filed: October 22, 2014
    Date of Patent: August 14, 2018
    Assignee: GLYTECH, INC.
    Inventors: Yasuhiro Kajihara, Yasuhito Morishita, Takefumi Murase
  • Patent number: 10000780
    Abstract: Disclosed are variants of Trichoderma reesei endoglucanase I, and methods for using such variants to break down cellulose and produce biofuel.
    Type: Grant
    Filed: November 21, 2014
    Date of Patent: June 19, 2018
    Assignees: IFP ENERGIES NOUVELLES, PROTEUS, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE—CNRS
    Inventors: Antoine Margeot, Yves Benoit, Cecile Persillon, Celine Ayrinhac, Christophe Ullmann, Olivier Bonzom, Sebastien Fort, Sylvie Armand, Maud Petit, Marine Lenon
  • Patent number: 9957492
    Abstract: Provided are isolated polypeptides having cellulolytic enhancing activity and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding modules. Also provided are nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding modules.
    Type: Grant
    Filed: June 28, 2013
    Date of Patent: May 1, 2018
    Assignee: Novozymes A/S
    Inventors: Lan Tang, Ye Liu, Yu Zhang
  • Patent number: 9896667
    Abstract: This application relates to laccase variants and uses thereof as eco-friendly biocatalysts in various industrial processes. More in particular, the disclosure relates to a polypeptide with laccase activity comprising an amino acid sequence that is at least 75% identical to the amino acid sequence according to SEQ ID NO: 1, wherein the polypeptide comprises an amino acid residue selected from the group consisting of threonine, proline, alanine, glycine, serine, cysteine, aspartic acid, asparagine and valine at a position corresponding to amino acid 149 of SEQ ID NO: 1.
    Type: Grant
    Filed: April 11, 2015
    Date of Patent: February 20, 2018
    Assignee: METGEN OY
    Inventor: Klara Birikh
  • Patent number: 9896707
    Abstract: Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.
    Type: Grant
    Filed: June 23, 2016
    Date of Patent: February 20, 2018
    Assignee: Battelle Energy Alliance, LLC
    Inventors: David N. Thompson, William A. Apel, Vicki S. Thompson, David W. Reed, Jeffrey A. Lacey, Emily D. Henriksen
  • Patent number: 9695407
    Abstract: The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions.
    Type: Grant
    Filed: March 19, 2015
    Date of Patent: July 4, 2017
    Assignee: DANISCO US INC
    Inventors: Luis Cascao-Pereira, Thijs Kaper, Bradley R. Kelemen, Amy D. Liu
  • Patent number: 9617301
    Abstract: Potent compounds having combined antioxidant, anti-inflammatory, anti-radiation and metal chelating properties are described. Short peptides having these properties, and methods and uses of such short peptides in clinical and cosmetic applications are described.
    Type: Grant
    Filed: April 21, 2015
    Date of Patent: April 11, 2017
    Assignees: ONEDAY-BIOTECH AND PHARMA LTD., Yissum Research Development Company of the Hebrew University of Jerusalem Ltd.
    Inventors: Josef Mograbi, Daphne Atlas, Shoshana Keynan
  • Patent number: 9551705
    Abstract: The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture fusions), surfaces (e.g., displaying, capture ligands, capture proteins, or capture fusions), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.
    Type: Grant
    Filed: December 12, 2013
    Date of Patent: January 24, 2017
    Assignee: Promega Corporation
    Inventors: Carolyn W. Hitko, Robin Hurst, Thomas Kirkland, Dieter Klaubert, Poncho Meisenheimer, Nidhi Nath, Rachel Friedman Ohana, Paul Otto, Marjeta Urh, Harry Tetsuo Uyeda, Keith Wood, Ji Zhu
  • Patent number: 9409949
    Abstract: Activation sites on the alpha subunit of sodium potassium ATPase have been discovered. It has also been discovered that certain antibodies that bind to the alpha subunit of sodium potassium ATPase dramatically increase enzyme activity. There has never before been a report of precise activation sites or drug interaction sites for sodium potassium ATPase. Certain methods have also been discovered for treating or preventing diseases associated with low sodium potassium ATPase activity by administering antibodies, antibody fragments and small molecules that bind to the activation sites on the alpha subunit of sodium potassium ATPase.
    Type: Grant
    Filed: May 3, 2013
    Date of Patent: August 9, 2016
    Inventor: Kai Yuan Xu
  • Patent number: 9297000
    Abstract: Provided are novel ?-neoagarobiose hydrolase and a method for obtaining a monosaccharide using the same.
    Type: Grant
    Filed: September 14, 2011
    Date of Patent: March 29, 2016
    Assignee: Korea University Research and Business Foundation
    Inventors: In Geol Choi, Kyoung Heon Kim, Sae Young Lee
  • Patent number: 9200264
    Abstract: This invention provides compositions of active highly phosphorylated lysosomal sulfatase enzymes, their pharmaceutical compositions, methods of producing and purifying such lysosomal sulfatase enzymes and compositions and their use in the diagnosis, prophylaxis, or treatment of diseases and conditions, including particularly lysosomal storage diseases that are caused by, or associated with, a deficiency in the lysosomal sulfatase enzyme.
    Type: Grant
    Filed: March 21, 2013
    Date of Patent: December 1, 2015
    Assignee: BIOMARIN PHARMACEUTICAL INC.
    Inventors: Erno Pungor, Charles Hague, Zhi Chen
  • Patent number: 9193962
    Abstract: The invention provides hydrolases, polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides, e.g., enzymes, having a hydrolase activity, e.g., an esterase, acylase, lipase, phospholipase (e.g., phospholipase A, B, C and D activity, patatin activity, lipid acyl hydrolase (LAH) activity) or protease activity, including thermostable and thermotolerant hydrolase activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The hydrolase activities of the polypeptides and peptides of the invention include esterase activity, lipase activity (hydrolysis of lipids), acidolysis reactions (to replace an esterified fatty acid with a free fatty acid), transesterification reactions (exchange of fatty acids between triglycerides), ester synthesis, ester interchange reactions, phospholipase activity and protease activity (hydrolysis of peptide bonds).
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: November 24, 2015
    Assignee: DSM IP ASSETS B.V.
    Inventors: Uwe T. Bornscheuer, David Weiner, Tim Hitchman, Jonathan Lyon, Sirirung Wongsakul
  • Publication number: 20150147783
    Abstract: The present invention relates to an isolated mutant eubacterium comprising at least one mutation resulting in a substitution of at least one amino acid in the beta-subunit of the RNA-polymerase encoded for by the rpoB-gene providing an altered production of a product of interest when said production of a product of interest is compared to the production of the same product in an isogenic wild type strain grown at identical conditions, wherein the substitution of at least one amino acid occurs at any of positions 469, 478, 482, 485, or 487 of SEQ ID NO:2, or at the equivalent positions in any eubacterial RNA-polymerase beta-subunit family member. Another aspect of the invention relates to a process for producing at least one product of interest in a mutant eubacterium and to a use of the mutant eubacterium according to the invention for producing at least one product of interest.
    Type: Application
    Filed: February 9, 2015
    Publication date: May 28, 2015
    Inventors: Steen Troels Joergensen, Jens Toenne Andersen, Niels Banke, Preben Nielsen
  • Publication number: 20150147780
    Abstract: Disclosed herein are embodiments for a novel method of producing an organic compound, including harvesting at least one organic compound from an organism or cell line genetically engineered with a gene for at least one proton-pump protein.
    Type: Application
    Filed: November 24, 2014
    Publication date: May 28, 2015
    Inventors: Eugene Dinescu, Vincent Dinescu
  • Publication number: 20150140636
    Abstract: The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.
    Type: Application
    Filed: May 22, 2014
    Publication date: May 21, 2015
    Applicant: Promega Corporation
    Inventors: Aldis Darzins, Lance P. Encell, Rachel Friedman Ohana, Paul Otto, Michael R. Slater, Gediminas Vidugiris, Keith V. Wood, Monika G. Wood, Kate Qin Zhao, Kris Zimmerman
  • Patent number: 9034612
    Abstract: The invention provides novel methods for making or modifying oils, e.g., plant animal or microbial oils, such as vegetable oils or related compounds, that are low in a particular fatty acid(s), for example, low linoleic oils, linolenic oils, low palmitic oils, low stearic oils or oils low in a combination thereof.
    Type: Grant
    Filed: May 24, 2013
    Date of Patent: May 19, 2015
    Assignee: DSM IP ASSETS, B.V.
    Inventors: David Lam, David Weiner, Tim S. Hitchman, Nelson R. Barton, Jonathan D. Lyon
  • Patent number: 9029107
    Abstract: It is an object of the present invention to provide a novel hydrolase, which is used when dialkyl 2-vinylcyclopropane-1,1-dicarboxylate is hydrolyzed with an enzyme, so as to efficiently obtain (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropanecarboxylic acid that is useful as an intermediate for synthesizing therapeutic agents for hepatitis C. According to the present invention, there is provided a hydrolase protein, which consists of the amino acid sequence shown in any one of SEQ ID NOS. 2 to 5 and which has activity of catalyzing, at higher selectivity than the protein consisting of the amino acid sequence shown in SEQ ID NO. 1, a reaction of producing (1S,2S)-1-ethoxycarbonyl-2-vinylcyclopropanecarboxylic acid from diethyl 2-vinylcyclopropane-1,1-dicarboxylate.
    Type: Grant
    Filed: August 31, 2011
    Date of Patent: May 12, 2015
    Assignee: API Corporation
    Inventors: Hiroshi Kawabata, Ryoma Miyake, Kuniko Asada, Ryouhei Katou
  • Patent number: 9029115
    Abstract: The pharmaceutical use of lipases related to the Thermomyces lanuginosus (Humicola lanuginosa) lipase comprising amino acids 1-269 of SEQ ID NO: 2, optionally in combination with a protease and/or an amylase. Examples of medical indications are: Treatment of digestive disorders, pancreatic exocrine insufficiency (PEI), pancreatitis, cystic fibrosis, diabetes type I, and/or diabetes type II. The lipases of the invention have, e.g., an improved digestion performance in vitro, an improved activity at a pH in the neutral range, an improved stability at low pH, and are stable against protease-degradation, and/or are stable in the presence of pepsin and bile salts. The invention also relates to methods of determining digestion performance in vitro of lipases, as well as to certain novel variants of the lipase of T. lanuginosus.
    Type: Grant
    Filed: August 16, 2012
    Date of Patent: May 12, 2015
    Assignees: Novozymes A/S, Novozymes Inc
    Inventors: Allan Svendsen, Michael Skjoet, Deborah Yaver, Lars Lehmann Hylling Christensen, Signe Eskildsen Larsen, Nina Lundin, Michael Lamsa, Peter Colin Gregory
  • Publication number: 20150125924
    Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
    Type: Application
    Filed: June 2, 2014
    Publication date: May 7, 2015
    Inventors: Aldis Darzins, Lance P. Encell, Dieter Klaubert, Georgyi V. Los, Mark McDougall, Keith V. Wood, Monika G. Wood, Chad Zimprich
  • Publication number: 20150118678
    Abstract: The present invention provides a novel assay that allows high-throughput screening of chemical compounds for the inhibition of binding between EF-Tu and tRNA.
    Type: Application
    Filed: October 31, 2014
    Publication date: April 30, 2015
    Inventors: Wlodek Mandecki, Emanuel Goldman, Maxim Chudaev
  • Publication number: 20150118216
    Abstract: Compositions, methods, strategies, kits, and systems for the delivery of negatively charged proteins, protein complexes, and fusion proteins, using cationic polymers or lipids are provided. Delivery of proteins into cells can be effected in vivo, ex vivo, or in vitro. Proteins that can be delivered using the compositions, methods, strategies, kits, and systems provided herein include, without limitation, enzymes, transcription factors, genome editing proteins, Cas9 proteins, TALEs, TALENs, nucleases, binding proteins (e.g., ligands, receptors, antibodies, antibody fragments; nucleic acid binding proteins, etc.), structural proteins, and therapeutic proteins (e.g., tumor suppressor proteins, therapeutic enzymes, growth factors, growth factor receptors, transcription factors, proteases, etc.), as well as variants and fusions of such proteins.
    Type: Application
    Filed: October 30, 2014
    Publication date: April 30, 2015
    Applicant: President and Fellows of Harvard College
    Inventors: David R. Liu, David B. Thompson, John Anthony Zuris
  • Patent number: 9017982
    Abstract: Provided are non-wild-type organophosphorus acid anhydrolases that are capable of degrading (ethyl {2-[bis(propan-2-yl)amino]ethyl}sulfanyl) (methyl)phosphinate and other V-agents. Particular embodiments include an organophosphorus acid anhydrolase including an amino acid substitution at position 212.
    Type: Grant
    Filed: December 17, 2013
    Date of Patent: April 28, 2015
    Assignee: The United States of America as Represented by the Secretary of the Army
    Inventors: Saumil S. Shah, Mark A. Guelta, Steven P. Harvey
  • Patent number: 9017981
    Abstract: An isolated human phosphatase and tensin homolog long polypeptide (PTEN-long) comprising SEQ ID NO:1, fragments and analogs thereof, nucleic acids encoding such and compositions comprising such are provided. Methods to inhibit angiogenesis in a solid tumor, treat a solid tumor, and inhibit growth of a solid tumor using PTEN-long, fragments and analogs thereof, are provided.
    Type: Grant
    Filed: February 17, 2010
    Date of Patent: April 28, 2015
    Assignee: The Trustees of Columbia University in the City of New York
    Inventor: Ramon Parsons
  • Patent number: 9012197
    Abstract: The present invention is directed to recombinant microalgal cells and their use in production of heterologous hemagglutinin-neuraminidase (HN) polypeptides, as well as compositions and uses thereof.
    Type: Grant
    Filed: December 28, 2010
    Date of Patent: April 21, 2015
    Assignees: Merial, Inc., Sanofi Vaccine Technologies, S.A.S.
    Inventors: Anne-Cécile V. Bayne, James Casey Lippmeier, Kirk Emil Apt, Xuan Guo, Joyce A. Pritchard
  • Patent number: 9012194
    Abstract: A method for producing sophorolipids having protein inducer and/or repressor activities having the steps of synthesizing the sophorolipid by fermentation of Candida bombicola in a fermentation media to form a natural mixture of lactonic sophorolipids and non-lactonic sophorolipids and then utilizing the natural mixture as a protein inducing agent, utilizing the natural mixture as a protein repressing agent, and/or utilizing the natural mixture as a combined protein induction/repressor agent. An application of the sophorolipid compound produced according to the method as a microbial media component.
    Type: Grant
    Filed: December 22, 2005
    Date of Patent: April 21, 2015
    Assignee: Synthezyme LLC
    Inventors: Richard A. Gross, Vishal Shah, Frantisek Nerud, Datta Madamwars
  • Patent number: 8999696
    Abstract: The present invention relates to methods for degrading or converting a cellulose-containing material, comprising: treating the cellulose-containing material with an effective amount of a cellulolytic enzyme composition comprising a polypeptide having cellulolytic enhancing activity, and one or more (several) components selected from the group consisting of a CEL7 polypeptide having endoglucanase activity, a CEL12 polypeptide having endoglucanase activity, a CEL45 polypeptide having endoglucanase activity, a CEL7 polypeptide having cellobiohydrolase activity with a cellulose binding domain, and a CEL7 polypeptide having cellobiohydrolase activity without a cellulose binding domain. The present invention also relates to such cellulolytic enzyme compositions.
    Type: Grant
    Filed: May 9, 2008
    Date of Patent: April 7, 2015
    Assignee: Novozymes, Inc.
    Inventor: Keith McFarland
  • Patent number: 8999653
    Abstract: The instant invention provides for methods for detecting the internalization of a transmembrane protein of interest expressed at the surface of a cell. More specifically, the methods involve (a) labelling the protein of interest with a fluorescent metal complex, the lifetime of which is greater than 0.1 ms, (b) adding to the reaction medium a composition capable of causing the internalization of the protein of interest, (c) adding to the reaction medium (1) a modulating agent which is a fluorescent FRET acceptor compound compatible with the fluorescent metal complex, the final concentration of which in the reaction medium is greater than 10?7M; or (2) a reducing agent, the redox potential of which is less than +0.1 V and preferably between 0.25 and 0.
    Type: Grant
    Filed: July 30, 2009
    Date of Patent: April 7, 2015
    Assignee: Cis-Bio International
    Inventors: Jurriaan Zwier, Robert Poole, Herve Ansanay, Michel Fink, Eric Trinquet
  • Patent number: 8999641
    Abstract: The invention provides for engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors with additional functional domains. Also provided are methods of directing CRISPR complex formation in prokaryotic and eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity.
    Type: Grant
    Filed: March 26, 2014
    Date of Patent: April 7, 2015
    Assignees: The Broad Institute Inc., Maassachusetts Institute of Technology, President and Fellows of Harvard College
    Inventors: Feng Zhang, Le Cong, Randall Jeffrey Platt, Neville Espi Sanjana
  • Patent number: 8999692
    Abstract: The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions.
    Type: Grant
    Filed: July 6, 2012
    Date of Patent: April 7, 2015
    Assignee: Danisco US Inc.
    Inventors: Luis G. Cascao-Pereira, Thijs Kaper, Bradley R. Kelemen, Amy D. Liu
  • Patent number: 8993276
    Abstract: Nucleic acid molecules encoding chimeric cellulase polypeptides that exhibit improved cellulase activities are disclosed herein. The chimeric cellulase polypeptides encoded by these nucleic acids and methods to produce the cellulases are also described, along with methods of using chimeric cellulases for the conversion of cellulose to sugars such as glucose.
    Type: Grant
    Filed: July 12, 2013
    Date of Patent: March 31, 2015
    Assignee: Alliance for Sustainable Energy, LLC
    Inventors: Qi Xu, John O. Baker, Michael E. Himmel
  • Patent number: 8993299
    Abstract: The present invention relates to polypeptides having cellobiohydrolase I activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.
    Type: Grant
    Filed: October 28, 2013
    Date of Patent: March 31, 2015
    Assignee: Novozymes A/S
    Inventors: Lene Lange, Wenping Wu, Dominique Aubert, Sara Landvik, Kirk Matthew Schnorr, Ib Groth Clausen
  • Patent number: 8993233
    Abstract: The invention provides for engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors with additional functional domains. Also provided are methods of directing CRISPR complex formation in prokaryotic and eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity.
    Type: Grant
    Filed: December 12, 2013
    Date of Patent: March 31, 2015
    Assignees: The Broad Institute Inc., Massachusetts Institute of Technology, President and Fellows of Harvard College
    Inventors: Feng Zhang, Le Cong, Randall Jeffrey Platt, Neville Espi Sanjana, Fei Ran
  • Patent number: 8986969
    Abstract: The present invention relates to polypeptides having cellobiohydrolase I activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.
    Type: Grant
    Filed: October 28, 2013
    Date of Patent: March 24, 2015
    Assignee: Novozymes A/S
    Inventors: Lene Lange, Wenping Wu, Dominique Aubert, Sara Landvik, Kirk Matthew Schnorr, Ib Groth Clausen
  • Publication number: 20150079602
    Abstract: The present invention refers to methods for selectively recognizing a base pair in a DNA sequence by a polypeptide, to modified polypeptides which specifically recognize one or more base pairs in a DNA sequence and, to DNA which is modified so that it can be specifically recognized by a polypeptide and to uses of the polypeptide and DNA in specific DNA targeting as well as to methods of modulating expression of target genes in a cell.
    Type: Application
    Filed: January 13, 2014
    Publication date: March 19, 2015
    Inventors: Ulla Bonas, Jens Boch, Sebastian Schornack, Thomas Lahaye
  • Publication number: 20150079095
    Abstract: The invention relates to generation and use of cellular and humoral responses for the prevention and treatment of P. gingivalis related conditions and diseases.
    Type: Application
    Filed: September 16, 2014
    Publication date: March 19, 2015
    Applicant: Oral Health Australia PTY Ltd
    Inventors: Eric Charles Reynolds, Neil Martin O'Brien Simpson, Keith J. Cross, Nada Slakeski
  • Patent number: 8980594
    Abstract: The invention relates to the use of a protein homologous to a MeaB protein for increasing the enzymatic activity of a 3-hydroxycarboxylic acid-CoA mutase, a fusion protein comprising a 3-hydroxycarboxylic acid-CoA mutase and a protein sequence homologous to a MeaB protein and an enzymatic method for producing 2-hydroxyisobutryric acid.
    Type: Grant
    Filed: October 11, 2010
    Date of Patent: March 17, 2015
    Assignee: Evonik Roehm GmbH
    Inventors: Liv Reinecke, Steffen Schaffer, Tim Koehler, Anja Thiessenhusen, Achim Marx, Markus Buchhaupt
  • Patent number: 8980609
    Abstract: An organophosphate scavenger is provided, with extended residence time in the mammalian circulation, which can be used in preventive and therapeutic treatment of organophosphate poisoning. The scavenger is a uniformly pegylated serine hydrolase, in which a part of lysine residues were replaced with other residues by site-directed mutagenesis. One part of lysine residues in the hydrolase amino acid sequence is selected for the PEG-coupling, and the other part for the replacement, wherein the selection should ensure that the hydrolase surface shows at least one free amino acid for PEG coupling for all possible views obtained by rotating a 3-D model generated for the hydrolase.
    Type: Grant
    Filed: June 8, 2006
    Date of Patent: March 17, 2015
    Assignee: Israel Institute for Biological Research
    Inventors: Ofer Cohen, Chanoch Kronman, Baruch Velan, Avigdor Shafferman
  • Patent number: 8975043
    Abstract: Novel enzymes, processes and antigenic structures useful in producing vaccines and compounds useful in combating gram-negative bacteria are described. Enzymes were isolated from the slime mold Dictyostelium discoideum and used to specifically degrade lipopolysaccharide (LPS). Enzymatic degradation permits residues of the LPS molecule, including immunogenic epitopes of the core oligosaccharide portion of the LPS, to remain unmodified during this enzymatic removal of fatty acids from the lipid A region of the LPS molecule.
    Type: Grant
    Filed: November 23, 2006
    Date of Patent: March 10, 2015
    Assignee: National Research Council of Canada
    Inventors: Andrew Cox, Dhamodharan Neelamegan, Ian Schoenhofen, Frank St. Michael, James Richards
  • Patent number: 8975063
    Abstract: The present invention relates to host cells that produce compounds that are characterized as benzylisoquinolines, as well as select precursors and intermediates thereof. The host cells comprise one, two or more heterologous coding sequences wherein each of the heterologous coding sequences encodes an enzyme involved in the metabolic pathway of a benzylisoquinoline, or its precursors or intermediates from a starting compound. The invention also relates to methods of producing the benzylisoquinoline, as well as select precursors and intermediates thereof by culturing the host cells under culture conditions that promote expression of the enzymes that produce the benzylisoquinoline or precursors or intermediates thereof.
    Type: Grant
    Filed: October 19, 2007
    Date of Patent: March 10, 2015
    Assignee: California Institute of Technology
    Inventors: D. Christina Smolke, Kristy Hawkins
  • Publication number: 20150064154
    Abstract: Methods and compositions related to the use of a protein with kynureninase activity are described. For example, in certain aspects there may be disclosed a modified kynureninase capable of degrading kynurenine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with kynurenine depletion using the disclosed proteins or nucleic acids.
    Type: Application
    Filed: August 29, 2014
    Publication date: March 5, 2015
    Applicant: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventors: George GEORGIOU, Everett STONE
  • Patent number: 8956845
    Abstract: The invention relates to recombinantly produced ?-glucosidase variants with enhanced thermoactivity compared to naturally occurring proteins. The invention also provides methods for producing a variant ?-glucosidase polypeptide with improved thermoactivity by identifying performance sensitive positions in a target ?-glucosidase polypeptide and substituting the residue at that position with a thermoactivity enhancing residue.
    Type: Grant
    Filed: May 28, 2014
    Date of Patent: February 17, 2015
    Assignee: Codexis, Inc.
    Inventors: Xiyun Zhang, Jie Yang, Dipnath Baidyaroy, Sally R. Postlethwaite, Attila Andor, Louis A. Clark, Lisa Marie Newman
  • Patent number: 8956842
    Abstract: The present invention relates to the novel Paenibacillus sp. strain, and the novel protein isolated from the same. More particularly, the present invention relates to the novel Paenibacillus sp. strain producing xylanase, and the novel xylanase having high activity at high temperature and in a wide range of pH, and a production method of the same. The Paenibacillus sp. HPL-3 strain (KCTC11987BP) and the xylanase of the present invention demonstrates high activity at high temperature or in a wide range of pH to decompose xylan, the major component of various lignocellulosic biomass, so that they can be effectively used for the production or development of bio-fuel, alternative material, performance chemical, bio-polymer, food and feeds, etc.
    Type: Grant
    Filed: August 12, 2011
    Date of Patent: February 17, 2015
    Assignee: Korea Research Institute of Chemical Technology
    Inventors: In Taek Hwang, No Joong Park, He Kyoung Lim
  • Publication number: 20150045284
    Abstract: The present invention relates to crystals of a type IB P-type ATPase having the space group P1 and methods for purification and growing said crystals. The invention also presents methods for identifying an inhibitor of a type IB P-type ATPase for example by determining binding interactions between an inhibitor and a set of binding interaction sites in said type IB P-type ATPase.
    Type: Application
    Filed: January 20, 2012
    Publication date: February 12, 2015
    Applicant: Aarhus Universitet
    Inventors: Poul Nissen, Pontus Gourdon, Xiang Liu, Bjorn Panyella Pedersen, Daniel Mattle
  • Patent number: 8951752
    Abstract: The invention provides isolated nucleic acid encoding one or more gene products that allow for degradation of caffeine and related structures, and for preparation of intermediates in that catabolic pathway. Further provided are methods of using one or more of those gene products.
    Type: Grant
    Filed: October 18, 2012
    Date of Patent: February 10, 2015
    Assignee: University of Iowa Research Foundation
    Inventors: Venkiteswaran Subramanian, Michael Tai-Man Louie, Ryan Summers
  • Patent number: 8945908
    Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.
    Type: Grant
    Filed: April 18, 2013
    Date of Patent: February 3, 2015
    Assignee: Solazyme, Inc.
    Inventors: Scott Franklin, Aravind Somanchi, George Rudenko, Riyaz Bhat, Xinhua Zhao, Risha Bond, Walter Rakitsky, Alejandro Marangoni, Diza Braksmayer
  • Patent number: 8945893
    Abstract: The present invention provides methods for producing derivatives from cultured cells. In addition, the present invention provides methods for conversion of prenyl derivatives, obtained from biological or petrochemical sources, to isoprene by employing chemical or biological catalysts. The present invention also provides compositions that include the cultured cells or isoprene or prenyl derivatives produced there from.
    Type: Grant
    Filed: June 6, 2013
    Date of Patent: February 3, 2015
    Assignees: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Joseph C. McAuliffe, Samira Rathnayake, Karl J. Sanford