Carboxylic Ester Hydrolase (3.1.1) Patents (Class 435/197)
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Patent number: 5621096Abstract: The invention provides a process for the preparation of the compound of formula I ##STR1## wherein R is carboxy group or a carboxylate anion, by enzymatic hydrolysis with a lipase or an acylase in an alcoholic solution, of a compound of the formula II ##STR2## wherein R.sub.1, represents a hydrogen atom or a C.sub.1 -C.sub.6 alkyl group, and R.sub.2 is a hydrogen atom, an alkyl, alkenyl, phcnyl, phenylalkyl or phenylalkenyl group having from 1 to 18 carbon atoms or an alkoxy group.Type: GrantFiled: November 16, 1995Date of Patent: April 15, 1997Assignee: Pharmacia S.p.A.Inventors: Walter Cabri, Angelo Bedeschi
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Patent number: 5618533Abstract: Diagnostic means and methods for Lyme disease comprising B. burgdorferi flagellin polypeptides and antibodies. Compositions and methods comprising neuroborreliosis-associated antigens useful for the detection, treatment and prevention of neuroborreliosis, arthritis, carditis and other manifestations of Lyme disease.Type: GrantFiled: December 10, 1993Date of Patent: April 8, 1997Assignee: Yale UniversityInventors: Richard A. Flavell, Erol Fikrig, Robert Berland
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Patent number: 5618687Abstract: An enzymatic process for the production of 7-amino cephalosporanic acid from cephalosporine C is disclosed. The process is a two-stage enzymatic reaction which can be performed in a single reactor. The invention further includes a mutant strain of Trigonopsis variabilis which produces increased amounts of D-amino acid oxidase, and a mutant strain of Acinetobacter sp., which increased amounts of deacylase, which are enzymes necessary for the present process.Type: GrantFiled: November 2, 1994Date of Patent: April 8, 1997Assignee: Biopure CorporationInventors: Bing L. Wong, Yong Q. Shen
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Patent number: 5604120Abstract: The present invention provides a method for producing optically active endo-2-norborneols, represented by the following formula: ##STR1## and the antipodes, it comprises asymmetrically hydrolysing racemic endo-2-acyloxynorbornane or endo-2-acyloxynorbornane, whose (R)-compound is in excess, having an optical purity of 50-95% ee with lipase derived from Candida genus. The optically active endo-2-norborneol useful for synthesizing intermediates of pharmaceutical preparations in large quantities.Type: GrantFiled: August 22, 1995Date of Patent: February 18, 1997Assignee: Chisso CorporationInventors: Hitoshi Yano, Teruyo Sugiura, Yasuyuki Koizumi, Naoyuki Yoshida
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Patent number: 5595903Abstract: Genetically engineered human acetylcholinesterase and antibodies that def the protein are described. These composition may be used in pharmaceutical preparations for treatment and prophylaxis of organo-phosphorous compound poisoning or post-operative apnea. Also described are human cholinesterase DNA probes which may be employed for diagnosing progressing ovarian carcinomas and hemocytopoietic disorders.Type: GrantFiled: August 2, 1993Date of Patent: January 21, 1997Assignee: Yissum Research Development Co. of the Hebrew University of JerusalemInventors: Hermona Soreq, Zakut Haim
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Patent number: 5593877Abstract: The present invention is directed to nucleic acids encoding vespid venom enzymes, or fragments thereof, recombinant vectors comprising such nucleic acids, and host cells containing the recombinant vectors. The invention is further directed to expression of such nucleic acids to produce recombinant vespid venom enzymes, or recombinant fragments, derivatives or analogs thereof. Such recombinant products are useful for diagnosis of allergy and for therapeutic treatment of allergy. In specific embodiments, the present invention provides nucleic acids encoding, and complete nucleotide and amino acids sequences for, vespid venom phospholipase, for example, Dolichovespula maculata phospholipase and Vespula vulgaris phospholipase, and vespid venom hyaluronidase, for example, Dolichovespula maculata hyaluronidase.Type: GrantFiled: January 11, 1994Date of Patent: January 14, 1997Assignee: The Rockefeller UniversityInventor: Te P. King
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Patent number: 5585256Abstract: The present invention relates to rhamnogalacturon acetyl esterases derived from Aspergillus aculeatus and DNA Sequences encoding the enzymes. The present invention also relates to the use of rhamnogalacturon acetyl esterases for degrading modified hairy region.Type: GrantFiled: October 5, 1994Date of Patent: December 17, 1996Assignee: Novo Nordisk A/SInventors: Kurt Dorreich, Flemming M. Christensen, Yvette Schnell, Marcel Mischler, Henrik Dalb.o slashed.ge, Hans P. Heldt-Hansen
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Patent number: 5571704Abstract: A process for the preparation of the compounds of formula ##STR1## (wherein R has the meanings reported in the specification) by enzymatic transesterification of enantiomeric mixtures is described.These compounds are intermediates useful in the synthesis of Diltiazem.Type: GrantFiled: March 14, 1995Date of Patent: November 5, 1996Assignee: Zambon Group S.p.A.Inventors: Luca Ghirotto, Stefano Servi, Claudio Fuganti, Angelo Gentile, Claudi o Giordano
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Patent number: 5559220Abstract: A DNA encoding an acetyl-coenzyme A carboxylase (ACCase) from a photosynthetic organism and functional derivatives thereof which are resistant to inhibition from certain herbicides. This gene can be placed in organisms to increase their fatty acid content or to render them resistant to certain herbicides.Type: GrantFiled: April 7, 1995Date of Patent: September 24, 1996Assignee: Midwest Research InstituteInventors: Paul G. Roessler, John B. Ohlrogge
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Patent number: 5538876Abstract: Carboxyl esterase is inactivated by several chemical compounds such as naproxen or diclofop. By substituting or modifying certain basic residues of the carboxyl esterase, this enzyme shows improved stability properties during application. In this way it is possible to perform stereospecific hydrolysis reactions on industrial scale even at high substrate concentrations.Type: GrantFiled: August 23, 1995Date of Patent: July 23, 1996Assignee: Gist-brocades, N.V.Inventors: Johanna H.G.M. Mutsaers, Cornelis J. Van Der Laken, Cornelis P. Broekhuizen, Wilhemus J. Quax
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Patent number: 5532152Abstract: The present invention provides purified and isolated polynucleotide sequences encoding human plasma platelet-activating factor acetylhydrolase. Also provided are materials and methods for the recombinant production of platelet-activating factor acetylhydrolase products which are expected to be useful in regulating pathological inflammatory events.Type: GrantFiled: June 6, 1995Date of Patent: July 2, 1996Assignee: ICOS CorporationInventors: Lawrence S. Cousens, Christine D. Eberhardt, Patrick Gray, Larry W. Tjoelker, Cheryl L. Wilder, Hai Trong
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Patent number: 5527697Abstract: An esterase with a molecular weight of 55,200 .+-.300 Da is suitable for the chemoselective conversion of a compound of the formula II into a compound of the formula I.Type: GrantFiled: July 12, 1994Date of Patent: June 18, 1996Assignee: Hoechst AktiengesellschaftInventors: Werner Aretz, Klaus Sauber
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Patent number: 5518903Abstract: The present invention provides a process for producing optically active 3-aminobutanoic acid characterized in that it comprises an asymmetrically hydrolyzing racemic ester of 3-substituted aminobutanoic acid in the presence of a hydrolase, obtaining an optically active ester of 3-substituted aminobutanoic acid and an optically active 3-substituted aminobutanoic acid of an enantiomer of the ester, and treating the above ester represented by the formula (II): ##STR1## to remove the protecting group. In addition, the new ester intermediates represented by the above formula (II) are provided.Type: GrantFiled: August 11, 1994Date of Patent: May 21, 1996Assignee: Chisso CorporationInventors: Naoyuki Yoshida, Teruyo Sugiura, Yasuyuki Koizumi
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Patent number: 5512454Abstract: O-acylated cephalosporins are produced by reacting 3-hydroxymethylcephalosporins with an acyl donor containing at least three carbon atoms and an enzyme which is a Rodosporidium toruloides esterase, a wheat germ lipase, an Aspergillus niger lipase, an orange peel acetylesterase or a Bacillus subtilis esterase. A preferred enzyme is the esterase from Rodosporidium toruloides ATCC 10657. The enzyme can be used while in whole cells or in soluble or inmobilized form.Type: GrantFiled: February 3, 1994Date of Patent: April 30, 1996Assignee: Bristol-Myers Squibb CompanyInventors: John J. Usher, Guna Romancik, Michael Politino, David A. Lowe
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Patent number: 5487996Abstract: A gene encoding an esterase originated from a microorganism of the genus Serratia, a mutant strain having high esterase productivity containing said gene, a recombinant plasmid comprising said gene inserted into a vector plasmid, a novel microorganism transformed with said recombinant plasmid, and a method for the production of an esterase which comprises cultivating the mutant strain or the novel microorganism as set forth above in a medium and collecting the produced esterase outside and inside the cells. Said mutant strain having high esterase productivity and new transformed microorganism have excellent esterase productivity and can produce the desired esterase in high purity on a large scale.Type: GrantFiled: July 11, 1994Date of Patent: January 30, 1996Assignee: Tanabe Seiyaku Co., Ltd.Inventors: Takeji Shibatani, Saburo Komatsubara, Kenji Omori, Hiroyuki Akatsuka
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Patent number: 5484725Abstract: A norbornane type ester hydrolase that enantio-selectively hydrolyzes a (.+-.)-exo-norbornane type ester represented by Formula I is provided: ##STR1## wherein R is acyl, and A and B are hydrogens, respectively, or where A and B are absent, resulting in a carbon-carbon double bond between the carbons to which A and B are attached in Formula I. The norbornane type ester hydrolase has an optimal pH of approximately 8 and a stable pH range of approximately 6 to 8.Type: GrantFiled: April 22, 1994Date of Patent: January 16, 1996Assignee: Shionogi & Co., Ltd.Inventors: Bunji Kageyama, Masanori Nakae, Shigeo Yagi
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Patent number: 5468632Abstract: The present invention provides isolated DNA compounds and recombinant DNA cloning and expression vectors that encode PNB esterase from Bacillus subtilis. The invention also provides host cells transformed with these vectors and a method for production of the PNB esterase by recombinant DNA techniques.Type: GrantFiled: September 22, 1993Date of Patent: November 21, 1995Assignee: Eli Lilly and CompanyInventors: Cathleen A. Cantwell, Roland L. Hodges, Derek McGilvray, Stephen W. Queener, James R. Swartling, Joseph M. Zock
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Patent number: 5445951Abstract: A process for the preparation of partially acylated derivatives of sucrose by the enzyme catalyzed deacylation of sucrose esters, wherein said process comprises treating a sucrose ester selected from the group consisting of sucrose octaacylate, sucrose heptaacylate, and sucrose hexaacylate in an anhydrous organic medium, with an enzyme or combination of enzymes capable of catalyzing the deacylation of said sucrose ester to produce a partially deacylated sucrose derivative having free hydroxyl group(s) in pre-selected position(s), and recovering the resulting partially deacylated sucrose derivative.Type: GrantFiled: October 7, 1993Date of Patent: August 29, 1995Assignee: McNeil-PPC, Inc.Inventors: David C. Palmer, Fernand Terradas
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Patent number: 5426043Abstract: Methods and DNA constructs are provided for the expression of a fungal acetyl xylan esterase gene in microbial hosts. A purified fungal acetyl xylan esterase is obtained which is suited for the use as an accessory enzyme in the degradation of acetylated xylans.Type: GrantFiled: March 16, 1992Date of Patent: June 20, 1995Assignee: Gist-Brocades, N.V.Inventors: Leendert H. De Graaff, Jacob Visser, Henriette C. Van Den Broeck, Francois Strozyk, Felix J. M. Kormelink, Johannes C. P. Boonman
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Patent number: 5418151Abstract: There is provided a regio-selective method for the resolution of carbohydrate monoester mixtures, by treating such mixtures with one or more selective enzymes. The resolution of such mixtures results in monoesters comprising significantly purer isolates of one desired isomer.Type: GrantFiled: August 7, 1992Date of Patent: May 23, 1995Assignee: Genencor International, Inc.Inventors: Charles T. Goodhue, Theresa C. Paulson, Robert Seemayer
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Patent number: 5405768Abstract: The present invention provides a novel approach to the production of restriction endonucleases. More specifically, there is provided a novel method for the overexpression of these enzymes, which comprises transforming a host cell with an expression vector containing DNA coding for the restriction endonuclease of interest and a vector for the expression of a methylase which is capable of protecting the host cell DNA against the restriction endonuclease of interest. The methylase vector is compatible with the restriction endonuclease expression vector. Preferably, the expression vector is a T7 expression vector and the cell is transformed with a third compatible vector which regulates the expression vector. Also disclosed is the cloning and overexpression of the AatII restriction endonuclease and methylase and the cloning and overexpression of the AluI restriction endonuclease and methylase.Type: GrantFiled: October 8, 1993Date of Patent: April 11, 1995Assignee: New England Biolabs, Inc.Inventors: Shuang-yong Xu, Donald O. Nwankwo
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Patent number: 5405763Abstract: An isolated gene encoding an esterase capable of asymmetrically hydrolysing an ester of chrysanthemic acid or its derivative to give an intermediate useful for the production of pharmaceutically and/or agriculturally useful compounds, expression plasmids containing said gene, microorganisms transformed with said expression plasmids, and the production of the esterase by culturing said transformants.Type: GrantFiled: January 9, 1992Date of Patent: April 11, 1995Assignee: Sumitomo Chemical Company, LimitedInventors: Masako Nishizawa, Hideo Okawa, Masaharu Kanaoka
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Patent number: 5380659Abstract: A process for highly regioselective esterification and ester cleavage on unsaturated sugar compounds with the aid of lipases and esterases, and products which can be prepared by this process.Highly regioselective esterifications and ester cleavages can be carried out on unsaturated sugar compounds with the aid of lipases and esterases. Lipases from microorganisms or from the pancreas and liver of animals are preferably used.Type: GrantFiled: August 6, 1992Date of Patent: January 10, 1995Assignee: Hoechst AktiengesellschaftInventors: Wolfgang Holla, Reinhold Keller
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Patent number: 5364768Abstract: An in vitro process for preparing 6((1R)-hydroxyethyl) penem acids by hydrolyzing the carboxylic ester derivative thereof using an enzyme capable of selectively hydrolyzing the ester group at the 3-position of the carboxylic acid ester using an esterase, acylase or lipase enzyme, wherein the enzymatic hydrolysis is effected at a pH in the range of from 5 to 8 and at a temperature in the range of 20.degree. C. to 40.degree. C.Type: GrantFiled: September 4, 1992Date of Patent: November 15, 1994Assignee: Farmitalia Carlo Erba S.r.l.Inventors: Maria Altamura, Franco Francalanci, Marcello Marchi
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Patent number: 5338676Abstract: A cephalosporin acetylhydrolase gene, a recombinant DNA molecule prepared by introducing said gene into a vector used in an E. coli host-vector system, E. coli cells transfomed with said recombinant DNA molecule, a protein having the amino acid sequence encoded by said gene, an enzyme which is a multimer of said protein, and a process for preparing said protein or enzyme are provided, said cephalosporin acetylhydrolase being an enzyme useful for converting cephalosporins such as cephalosporin C and 7-ACA into deacetylated ones such as deacetylcephalosporin C and deacetyl 7-ACA which are useful as an intermediate for preparing a variety of derivatized cephalosporin antibiotics.Type: GrantFiled: November 25, 1992Date of Patent: August 16, 1994Assignee: Shionogi & Co., Ltd.Inventors: Kenji Mitsushima, Akio Takimoto, Shigeo Yaqi, Takayasu Sonoyama
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Patent number: 5312743Abstract: For the preparation of cholesterol esterases with variable substrate specificity by cloning of a cholesterol esterase gene, the active center of which has the sequence -Gly-His-Ser-X-Gly-, wherein X signifies an amino acid, into a vector, transformation of a micro-organism with this vector and expression of the cholesterol esterase gene, one exchanges the amino acid X of the active center for another amino acid by mutagenesis.Type: GrantFiled: January 29, 1992Date of Patent: May 17, 1994Assignee: Boehringer Mannheim GmbHInventors: Gunther Schumacher, Christian Hanke, Stephan Fischer
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Patent number: 5304631Abstract: The design, and synthesis of peptide-based molecules termed helizymes which possess catalytic activity are described herein The catalytic molecules of the invention comprise a number of amphiphilic helical peptides, which interact via hydrophobic interactions and which are bonded at their carboxyl ends to a multifunctional base. Active site residues functional for catalysis are positioned within or at the N-termini of the helical peptides. The helizyme molecule adopts a conformation in aqueous medium such that a substrate binding pocket is formed and such that functional active site geometry results from the association of the helical peptides Specifically exemplified helizymes possess specificities and at least one catalytic activity of chymotrypsin, trypsin and acetylcholine esterase.Type: GrantFiled: January 16, 1990Date of Patent: April 19, 1994Assignee: University of Colorado Foundation, Inc.Inventors: John M. Stewart, Karl W. Hahn, Wieslaw A. Klis
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Patent number: 5281520Abstract: Methods are disclosed for producing acyloxyacyl hydrolase. The protein is produced from eukaryotic host cells transformed or transfected with DNA construct(s) containing information necessary to direct the expression of acyloxyacyl hydrolase. The DNA constructs generally include the following operably linked elements: a transcriptional promoter; DNA sequence encoding acyloxyacyl hydrolase, the small subunit of acyloxyacyl hydrolase or the large subunit of acyloxyacyl hydrolase; and a transcriptional terminator. In addition, isolated DNA sequences encoding acyloxyacyl hydrolase and isolated DNA sequences encoding the small or large subunit of acyloxyacyl hydrolase are disclosed.Type: GrantFiled: September 12, 1990Date of Patent: January 25, 1994Assignees: ZymoGenetics, Inc., Board of Regents University of Texas SystemInventors: Patrick J. O'Hara, Frederick S. Hagen, Francis J. Grant, Robert S. Munford
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Patent number: 5281525Abstract: A cephalosporin acetylhydrolase gene, a recombinant DNA molecule prepared by introducing said gene into a vector used in an E. coli host-vector system, E. coli cells transformed with said recombinant DNA molecule, a protein having the amino acid sequence encoded by said gene, an enzyme which is a multimer of said protein, and a process for preparing said protein or enzyme are provided, said cephalosporin acetylhydrolase being an enzyme useful for converting cephalosporins such as cephalosporin C and 7-ACA into deacetylated ones such as deacetylcephalosporin C and deacetyl 7-ACA which are useful as an intermediate for preparing a variety of derivatized cephalosporin antibiotics.Type: GrantFiled: April 22, 1991Date of Patent: January 25, 1994Assignee: Shionogi & Co., Ltd.Inventors: Kenji Mitsushima, Akio Takimoto, Shigeo Yagi, Takayasu Sonoyama
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Patent number: 5278054Abstract: A process for the enzymatic hydrolysis of a carboxylic acid derivative by dissolving the carboxylic acid derivative in an organic solvent which is miscible with water only to a slight extent, saturation of the organic solution with water, bringing the water-saturated organic solution into contact with a hydrolase, the hydrolysis taking place, after which the reaction solution is saturated with water again and brought into contact with the hydrolase and subsequently with water again until the desired degree of conversion is achieved.Type: GrantFiled: April 17, 1992Date of Patent: January 11, 1994Assignee: Chemie Linz Gesellschaft m.b.H.Inventors: Maria Buchner, Robert Estermann, Herbert Mayrhofer, Gerald Banko
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Patent number: 5272080Abstract: Butyrylcholinesterase is produced in a purity of at least 90% by subjecting plasma fraction IV-4 alone or in admixture with fraction IV-1 to both anion exchange chromatography and affinity chromatography.Type: GrantFiled: February 19, 1991Date of Patent: December 21, 1993Assignee: Pharmavene, Inc.Inventor: Thomas J. Lynch
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Patent number: 5270206Abstract: Process for the pancreatic lipase mediated transesterification method for the optical resolution of endo-norborneol; derived optically active 5-(3-(exo-bicyclo[2.2.1]hept-2-yloxy)-4-methoxyphenyl)-3,4,5,6-tetrahydrop yrimidin-2(1H)-ones; and stepwise process and intermediate therefor.Type: GrantFiled: May 8, 1992Date of Patent: December 14, 1993Assignee: Pfizer Inc.Inventor: Nicholas A. Saccomano
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Patent number: 5250426Abstract: Monoclonal antibodies or paratope-containing portions thereof are disclosed that immunoreact with an enol ester substrate ligand having a prochiral center and catalytically hydrolyze a single predetermined eThis invention was made with government support under Contract GM 43858 awarded by the National Institutes of Health. The government has certain rights in the invention.Type: GrantFiled: October 22, 1991Date of Patent: October 5, 1993Assignee: The Scripps Research InstituteInventors: Richard A. Lerner, Kim Janda
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Patent number: 5248609Abstract: The invention relates to a process for the production of optically active (-)-oxabicyclo[3.3.0]octanolones of formula (-)-I ##STR1## wherein R is defined herein, comprising subjecting a racemic mixture of oxabicyclo[3.3.0]octanolone acylate to stereospecific acylate hydrolysis and separating the resultant products.Type: GrantFiled: April 8, 1991Date of Patent: September 28, 1993Assignee: Schering AktiengesellschaftInventors: Karl Petzoldt, Helmut Dahl
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Patent number: 5248604Abstract: The subject invention provides an enzymatically active nonglycosylated, recombinant, human acetylcholinesterase comprising at least one polypeptide characterized by an amino acid sequence which is substantially identical to the amino acid sequence of naturally-occurring human acetylcholinesterase. The subject invention additionally provides an enzymatically active recombinant human acetylcholinesterase comprising at least one polypeptide characterized by an amino acid sequence in which serine is substituted for cys 611 in the sequence of naturally-occurring, human acetylcholinesterase and an enzymatically active recombinant human acetylcholinesterase comprising at least one polypeptide characterized by the presence of a methionine of the N-terminus of the amino acid sequence of naturally-occurring human acetylcholinesterase.Type: GrantFiled: July 22, 1991Date of Patent: September 28, 1993Assignee: Bio-Technology General Corp.Inventor: Meir Fischer
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Patent number: 5242807Abstract: A recombinant DNA molecule consisting of segments of DNA from different genomes which have been joined end-to-end outside of living cells, and the progeny of such a recombinant DNA molecule, which recombinant DNA molecule (1) has the capacity to infect a host cell and to be maintained therein, and (2) comprises a DNA coding sequence selected from the group consisting of:(a) the CLCP-encoding sequence shown in FIG. 1,(b) a DNA sequence which hybridizes to the CLCP-encoding sequence shown in FIG. 1, and which encodes a polypeptide which (i) cross-reacts with an antibody to CLCP or to denatured CLCP, (ii) exhibits lysophospholipase activity, and (iii) forms dipyramidal crystals under conditions permitting CLC formation; and(c) a DNA sequence encoding a polypeptide encoded by any of the foregoing DNA sequences.Type: GrantFiled: November 1, 1989Date of Patent: September 7, 1993Assignee: Beth Israel Hospital AssociationInventors: Steven J. Ackerman, Helene F. Rosenberg, Daniel G. Tenen
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Patent number: 5238831Abstract: Carboxyl esterase is inactivated by several chemical compounds such as naproxen or diclofop. By substituting or modifying certain basic residues of the carboxyl esterase, this enzyme shows improved stability properties during application. In this way it is possible to perform stereospecific hydrolysis reactions on industrial scale even at high substrate concentrations.Type: GrantFiled: May 14, 1992Date of Patent: August 24, 1993Assignee: Gist-brocadesInventors: Johanna H. G. M. Mutsaers, Cornelis J. van der Laken, Cornelis P. Broekhuizen, Wilhemus J. Quax
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Patent number: 5234821Abstract: A 1,4-dihydropyridine derivative represented by formula (I): ##STR1## wherein X represents an alkyl group, or a group of ##STR2## in which R.sub.1, R.sub.2, and R.sub.3 may be the same or different and each represent a hydrogen atom, a halogen atom, a nitro group, a nitrile group, or a trifluoromethyl group; R.sub.4 represents a substituted or unsubstituted acyloxymethyl group, an alkoxycarbonyloxymethyl group, a (2-oxo-1,3-dioxolen-4 -yl)methyl group, a (5-substituted-2-oxo-1,3-dioxolen-4 -yl)methyl group, or an acyl group; R.sub.5 represents a lower alkyl group or a substituted alkyl group; and R.sub.6 represents a hydrogen atom, a lower alkoxymethyl group, or a lower acyloxymethyl group, is disclosed. The compound is stereospecifically hydrolyzed by the action of an enzyme to provide an optically active 1,4-dihydro -3-pyridinemonocarboxylic acid which is useful as an intermediate of pharmaceuticals in good optical purity and yield.Type: GrantFiled: August 30, 1991Date of Patent: August 10, 1993Inventor: Kazuo Achiwa
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Patent number: 5232852Abstract: Alcohols of the formula ##STR1## wherein R.sup.1 and R.sup.2 are each independently methyl or ethyl or together signify pentamethylene,formed by the enzymatic hydrolysis of a corresponding (RS) alkanoic acid ester are intermediates for Vitamin E.Type: GrantFiled: March 13, 1990Date of Patent: August 3, 1993Assignee: Hoffmann-La Roche Inc.Inventors: Richard Barner, Josepf Hubscher, Beat Wirz
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Patent number: 5232696Abstract: A method and apparatus for reducing the levels of low density lipoproteins (LDL) in blood is disclosed. The LDL is contacted with an enzyme which modifies it in a manner such that the LDL is rapidly removed endogenously by the patients' own metabolic processes. The enzyme may be introduced into the patient by injection, transdermal transport, nasal insufflation and ingestion. Additionally, the enzyme may be contained in a reactor for both in vivo and extracorporeal use.Type: GrantFiled: September 25, 1987Date of Patent: August 3, 1993Assignee: Massachusetts Institute of TechnologyInventors: Robert S. Lees, Robert S. Langer, Jr., Claudy J. P. Mullon, Hugh D. Conlon
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Patent number: 5229280Abstract: Continuous biotechnological preparation of optical isomer S(+) of 2-(6-methoxy-2-naphthyl) propionic acid consisting in the reaction of a racemic (R,S) ester of 2-(6-methoxy-2-naphthyl) propionic acid having melting point below 50.degree. C. of formula: ##STR1## wherein R.sub.1 =a C.sub.2 -C.sub.10 alkyl, C.sub.4 -C.sub.6 cycloalkyl, phenyl, tetrahydropyranyl , tetrahydrofuranyl radicals and--(CH.sub.2 CH.sub.2 O).sub.n --R.sub.2 group, whereinn=2-15 andR.sub.2 =a C.sub.1 -C.sub.4 alkyl group, with the Lipase obtained from Candida Cylindracea, at temperatures ranging from 20.degree.-60.degree. C. and at a pH ranging from 5 to 8, by feeding said ester in a continuous way into a bioreactor containing said Lipase immobilized on a porous carrier selected from adsorbent resins of the polyacrylester type having a porosity ranging from 50 to 1000 .ANG. and a granulometry ranging from 1 to 0.01 mm.Type: GrantFiled: February 21, 1989Date of Patent: July 20, 1993Assignee: Istituto Guido Donegani S.p.A.Inventors: Daniele Bianchi, Pietro Cesti, Carlo Pina, Ezio Battistel
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Patent number: 5219733Abstract: A process for reacting(1) a component selected from the group consisting of sterols and branched aliphatic primary or secondary alcohols having 14 to 32 carbon atoms, and(2) a component selected from the group consisting of fatty acids and fatty acid estersin contact with an enzyme selected from the group consisting of lipase and cholesterol esterase or with the selected enzyme in an immobilized form, in a system selected from the group consisting of an aqueous medium and water-containing organic solvent to prepare a fatty acid ester of the component (1).Type: GrantFiled: August 7, 1990Date of Patent: June 15, 1993Assignee: Yoshikawa Oil & Fat Co., Ltd.Inventors: Katsunori Myojo, Youichi Matsufune, Shiro Yoshikawa
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Patent number: 5219744Abstract: A process for modifying fats and oils which comprises applying partial glyceride lipase to fats and oils containing partial glycerides and fatty acids to synthesize triglycerides from the partial glycerides by esterification.The lipase is preferably an immobilized lipase and this process is preferably carried out under a water concentration of 1500 ppm or less.By using partial glyceride lipase according to the present invention, there can be obtained modified fats and oils having a low content of diglycerides without changing triglyceride composition.Type: GrantFiled: June 26, 1991Date of Patent: June 15, 1993Assignees: Ajinomoto Co., Inc., Amano Pharmaceutical Co., Ltd.Inventors: Jun Kurashige, Narihide Matsuzaki, Tamio Mase, Shotaro Yamaguchi
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Patent number: 5200324Abstract: A method is provided for the screening of senile dementia of the Alzheimer type (Alzheimer's disease) by testing for an anomalous molecular form of acetylcholinesterase in cerebrospinal fluid of a patient.Type: GrantFiled: August 1, 1991Date of Patent: April 6, 1993Assignee: E. R. Squibb & Sons, Inc.Inventors: Dasakumar S. Navaratnam, John D. Priddle, Brendon I. McDonald, A. David Smith, Kim A. Jobst
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Patent number: 5187086Abstract: An antibody molecule or molecule containing antibody combining site portions (catalytic molecule) that catalytically hydrolyzes a preselected carboxylic acid amide or ester bond of a reactant ligand, methods of making and using the catalytic molecule, and cells that produce those molecules are disclosed. The catalytic molecules bind to a reactant ligand containing the bond to be hydrolyzed and also to a haptenic ligand. The haptenic ligand is structurally analogous to the reactant ligand and contains a tetrahedral carbon atom that is bonded to a hydroxyl group and to a saturated carbon atom at a position in the haptenic ligand that corresponds to position of the carbonyl group and its bonded heteroatom of the reactant ligand. The haptenic ligand also contains a group that bears an ionic charge in aqueous solution at physiological pH values that is not present at a corresponding position of the reactant ligand. The ionic charge-bearing group is located in the hapten within 7 .ANG.Type: GrantFiled: January 23, 1991Date of Patent: February 16, 1993Assignee: Scripps Clinic and Research FoundationInventor: Kim Janda
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Patent number: 5182198Abstract: A process for preparing (+)-homopilopic acid and the salts thereof which comprises hydrolyzing a mixture of (+)-homopilopic acid ester of the general formula (I): ##STR1## wherein R.sub.1 denotes straight or branched C.sub.1 -C.sub.10 hydrocarbon, and (-)-homopilopic acid ester of the general formula (II): ##STR2## wherein R.sub.2 denotes straight or branched C.sub.1 -C.sub.10 hydrocarbon, in the presence of a microorganism belonging to the genus Arthrobacter, Aspergillus, Escherichia, Cunninghamella, Xanthomonas, Candida, Pseudomonas, Serratia, Cellulomonas, Nocardia, Bacillus, Brevibacterium, Flavobacterium, Mycobacterium, Rhizomucor, Rhodotorula or Rhodococcus, or a treated matter thereof.Type: GrantFiled: November 16, 1990Date of Patent: January 26, 1993Assignee: Mitsui Petrochemical Industries, Ltd.Inventors: Shoichi Kise, Mikio Hayashida, Aiichiro Ori, Junzou Hiratsuka, Hideaki Yamada
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Patent number: 5175100Abstract: 2-Arylpropionic acids such as ketoprofen, ibuprofen and naproxen can be stereospecifically resolved from their esters by hydrolysis using liver enzymes and particularly liver acetone powders derived from specific animals such as dog, pigeon, horse and goat.Type: GrantFiled: June 24, 1991Date of Patent: December 29, 1992Assignee: Rhone-Poulenc, Inc.Inventor: Animesh Goswami
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Patent number: 5162201Abstract: Disclosed herein is a novel monoglygeride lipase at least capable of catalyzing an enzymatic reaction of the following equation (a) and as substrate specificity, capable of acting on monoglyceride but incapable of acting on diglyceride and triglyceride:Monoglyceride+H.sub.2 O.fwdarw.Glycerol+Fatty acid (a)The monoglyceride lipase is produced by culturing a specific monoglyceride lipase producing microorganism of Bacillus and then collecting the monoglyceride lipase from the resulting culture. A method is also disclosed for the analysis of a monoglyceride-containing sample solution. The monoglyceride lipase is caused to act on the sample solution upon measurement of the monoglyceride in the sample solution. Either one of glycerol and the fatty acid formed in accordance with the equation (a) is then measured.Type: GrantFiled: March 12, 1991Date of Patent: November 10, 1992Assignee: Toyo Jozo Co., Ltd.Inventors: Shigeyuki Imamura, Mamoru Takahasi, Hideo Misaki, Kazuo Matsuura
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Patent number: 5155028Abstract: In the enzymatic stereoselective ester cleavages of 2-arylpropionate, the reaction rate of the hydrolyzing enzymes can be drastically increased if the vinyl ester of the 2-arylpropionate is employed as the substrate.Type: GrantFiled: June 8, 1990Date of Patent: October 13, 1992Assignee: Hoechst AktiengesellschaftInventors: Gerd Fulling, Merten Schlingmann, Reinhold Keller
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Patent number: 5106736Abstract: An enzymatic process for the enantiomer-specific preparation of mercapto alkanoic acids by stereoselective hydrolysis of mercapto or thioester alkanoic acid esters.Type: GrantFiled: April 23, 1991Date of Patent: April 21, 1992Assignee: E.R. Squibb & Sons, Inc.Inventors: Ramesh N. Patel, Laszlo J. Szarka