Ribonuclease (3.1.4) Patents (Class 435/199)
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Publication number: 20040152079Abstract: Compositions comprising tRNA synthetase polypeptides useful for regulating angiogenesis, as well as nucleic acids encoding such tRNA synthetase polypeptides are described. Methods of making and using such compositions are also disclosed.Type: ApplicationFiled: September 30, 2002Publication date: August 5, 2004Inventors: Paul Schimmel, Keisuke Wakasugi
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Publication number: 20040146987Abstract: A fusion polypeptide comprising a protein of interest which has a reduced half-life of expression, and a nucleic acid molecule encoding the fusion polypeptide, are provided.Type: ApplicationFiled: September 16, 2003Publication date: July 29, 2004Applicant: Promega CorporationInventors: Alexey Zdanovsky, Marina Zdanovskaia, Dongping Ma, Keith V. Wood, Brian Almond, Monika G. Wood
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Publication number: 20040147006Abstract: Isolated peptides are provided that span the Simian Virus 40 DNA region of the T-ag (or the Bovine Papillomavirus E1 protein) centered on position Thr124 (or Thr102, respectively), which include regulatory motifs, such as the nuclear localization signal (NLS) and the consensus recognition site for cyclin-Cdk kinases. When unphosphorylated, peptides derived from this region bind to DNA and inhibit T-ag assembly. Upon phosphorylation at Thr124 (or Thr102), these peptides neither bind to DNA nor to inhibit T-ag assembly. These forms of regulation of DNA replication and T-ag assembly provide a novel target, and provide screens for anti-viral agents, and for gene therapy.Type: ApplicationFiled: November 5, 2003Publication date: July 29, 2004Inventor: Peter Bullock
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Publication number: 20040142376Abstract: The present invention provides cDNAs encoding deoxyribonuclease II&bgr; and isolated, purified deoxyribonuclease II&bgr; proteins. Antibodies against this protein and antisense agents targeted to a cDNA or corresponding mRNA encoding deoxyribonuclease II&bgr; are provided. In addition, methods of identifying and using modulators of deoxyribonuclease II&bgr; activity are described.Type: ApplicationFiled: March 1, 2004Publication date: July 22, 2004Inventors: Alan Richard Eastman, Ronald J. Krieser
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Publication number: 20040142892Abstract: This invention provides methods, nucleic acids, compounds, and compositions for expressing a product of interest in a cell that involve a secretable RNA Polymerase.Type: ApplicationFiled: October 16, 2003Publication date: July 22, 2004Applicant: The University of British ColumbiaInventors: John Finn, Ian MacLachlan
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Publication number: 20040141994Abstract: A Herpes simplex virus (HSV) recombinase comprises a purified or isolated alkaline nuclease and a single stranded DNA binding protein. In HSV-1, the alkaline nuclease is the UL12 protein and the single stranded DNA binding protein is the ICP8 protein. The HSV recombinase can be purified from an in vitro expression system or can be expressed in an appropriate vector or vectors wherein the DNAs encoding the polypeptides are operatively linked to expression control sequences. Methods of use of the HSV recombinase include cloning, treating cells and organisms, and producing transgenic animals. The HSV recombinase can be in the form of a kit useful for cloning.Type: ApplicationFiled: September 4, 2003Publication date: July 22, 2004Inventors: Sandra Weller, Richard S. Myers, Nina Bacher Reuven
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Publication number: 20040142364Abstract: The present invention relates to isolated nucleic acid sequences encoding polypeptides having transcriptional activation activity and to the polypeptides. The invention also relates to nucleic acid constructs, vectors and host cells comprising the nucleic acid sequences. The invention further relates to host cells useful for the production of polypeptides in which the production or function of the transcriptional activator has been altered, as well as to methods for producing the polypeptides.Type: ApplicationFiled: December 5, 2003Publication date: July 22, 2004Applicant: Novozymes A/SInventors: Carsten M. Hjort, C.A.M.J.J. van den Hondel, P.J. Punt, F.H.J. Schuren, Tove Christensen
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Publication number: 20040142449Abstract: The present invention relates to functional, replication-competent full-length proviral PERV-A and PERV-B clones isolated directly from the pig genome, i.e. “native” PERV and allows the comparison of proviral PERV sequences from different origins on the molecular, structural and cellular level.Type: ApplicationFiled: December 17, 2003Publication date: July 22, 2004Inventors: Ralf R. Tonjes, Ulrich Krach, Marcus Niebert
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Patent number: 6764843Abstract: The present invention relates to recombinant DNA that encodes the BsmBI restriction endonuclease as well as BsmBI methyltransferase, expression of BsmBI restriction endonuclease and BsmBI methylase in E. coli cells containing the recombinant DNA. It also relates to a method for purification of the recombinant BsmBI restriction endonuclease.Type: GrantFiled: September 28, 2001Date of Patent: July 20, 2004Assignee: New England Biolabs, Inc.Inventors: Shuang-yong Xu, Andrew Dore, Adam Hume, John Pelletier, Jing Zhou
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Publication number: 20040137595Abstract: MFQ-111 polypeptides and polynucleotides and methods for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing MFQ-111 polypeptides and polynucleotides in diagnostic assays.Type: ApplicationFiled: February 19, 2003Publication date: July 15, 2004Inventors: Marc Masa Alvarez, Blanca Rodes Gubern, Ramon Messeguer Peypoch, Elisabet Rosell Vives, Jaume Piulats Xanco
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Publication number: 20040137425Abstract: The invention concerns the use of intramolecularly, covalently cross-linked proteins and covalently cross-linked reverse transcriptase from HIV as immunological binding partners in immunoassays. It also concerns immunological test procedures for detecting an analyte in a sample in which intramolecularly, covalently cross-linked proteins are used as binding partners, and it further concerns intramolecularly, covalently cross-linked reverse transcriptase from HIV and a method for producing this reverse transcriptase.Type: ApplicationFiled: May 28, 2003Publication date: July 15, 2004Applicant: Roche Diagnostics CorporationInventors: Barbara Upmeier, Dittmar Schlieper, Frederic Donie
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Publication number: 20040132009Abstract: The present invention relates to a highly processive reverse transcriptase having DNA polymerase activity and substantially reduced protease activity. More specifically, the invention relates to an isolated reverse transcriptase from foamy virus comprising a substantially inactivated protease. The invention also relates to vectors containing the gene and hosts transformed with the vector of the invention. Further, the invention relates to a method for producing reverse transcriptase having DNA polymerase activity and substantially reduced protease activity by expressing the reverse transcriptase genes of the present invention in a recombinant host. Methods are also provided for producing cDNA from polynucleotides using the highly processive reverse transcriptase of the invention. Kits for the preparation of cDNA from RNA comprising the highly processive reverse transcriptase of the invention are also provided.Type: ApplicationFiled: November 21, 2003Publication date: July 8, 2004Inventors: Stephen Hughes, Paul Boyer, Maxine Linial, Carolyn Rinke, Patrick Clark
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Publication number: 20040132985Abstract: The present invention relates to thermostable DNA polymerases derived from the hyperthermophilic eubacteria, and Thermotoga neapolitana in particular. The present invention provides means for isolating and producing the enzymes from these thermostable DNA polymerases, which are useful in many recombinant DNA techniques, especially such techniques as thermal cycle sequencing and nucleic acid amplification.Type: ApplicationFiled: November 12, 2003Publication date: July 8, 2004Inventors: Elena Bolchakova, Alexander Novikov, Michael R. Slater, Fen Huang, James R. Hartnett, Galina A. Velikodvorskaya
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Publication number: 20040132129Abstract: The present invention relates to recombinant DNA which encodes the Tth111II restriction endonuclease-methylase fusion protein (Tth111IIRM), expression of Tth111II restriction endonuclease-methylase fusion protein in E. coli cells containing the recombinant DNA, and purification of Tth111II endonuclease-methylase fusion protein to near homogeneity.Type: ApplicationFiled: January 8, 2003Publication date: July 8, 2004Applicant: New England Biolabs, Inc.Inventors: Zhenyu Zhu, Derek Robinson, Jack Benner, Shuang-Yong Xu
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Patent number: 6759575Abstract: An improved barstar gene and improved barstar protein can be used to neutralize the activity of a barnase in eukaryotic cells, particular in plant cells. The improved barstar gene can be used to produce fertility restorer plants capable of restoring the fertility to a line of male-sterile plants that contain in the nuclear genome of their cells a chimeric gene comprising a stamen-selective promoter and a DNA coding for a barnase. Restorer plants containing the barstar gene in the restorer plant's nuclear genome is also disclosed.Type: GrantFiled: October 5, 2001Date of Patent: July 6, 2004Assignee: Bayer Bioscience N.V.Inventors: Frank Michiels, Mark Williams
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Patent number: 6759226Abstract: The present invention relates to novel enzymes designed for direct detection, characterization and quantitation of nucleic acids, particularly RNA. The present invention provides enzymes that recognize specific nucleic acid cleavage structures formed on a target RNA sequence and that cleave the nucleic acid cleavage structure in a site-specific manner to produce non-target cleavage products. The present invention provides enzymes having an improved ability to specifically cleave a DNA member of a complex comprising DNA and RNA nucleic acid strands.Type: GrantFiled: May 24, 2000Date of Patent: July 6, 2004Assignee: Third Wave Technologies, Inc.Inventors: Wu-Po Ma, Victor I. Lyamichev, Michael W. Kaiser, Natalie E. Lyamicheva, Hatim Taysir Allawi, James J. Schaefer, Bruce P. Neri
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Publication number: 20040127480Abstract: The invention features novel RNase P molecules and nucleic acids encoding the same. Methods for discovery of antimicrobial compounds are also featured.Type: ApplicationFiled: March 1, 2001Publication date: July 1, 2004Inventors: Venkat Gopalan, Milan Jovanovic, Paul S. Eder, Tony Giordano, Gordon D. Powers, K. Asish Xavier
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Publication number: 20040126867Abstract: The present invention provides polynucleotides encoding human RNAse III and polypeptides encoded thereby. Methods of using said polynucleotides and polypeptides are also provided.Type: ApplicationFiled: February 9, 2004Publication date: July 1, 2004Inventors: Stanley T. Crooke, HongJiang Wu
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Publication number: 20040126866Abstract: The present invention relates to novel purified and isolated nucleotide sequences encoding mammalian Ca2+/calmodulin stimulated phosphodiesterases (CaM-PDEs) and cyclic-GMP-stimulated phosphodiesterases (cGS-PDEs). Also provided are the corresponding recombinant expression products of said nucleotide sequences, immunological reagents specifically reactive therewith, and procedures for identifying compounds which modulate the enzymatic activity of such expression products.Type: ApplicationFiled: October 30, 2003Publication date: July 1, 2004Inventors: Joseph A. Beavo, J. Kelley Bentley, Harry Charbonneau, William K. Sonnenburg
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Publication number: 20040126753Abstract: This invention relates to a method for producing virus RNA polymerases of RNA viruses, more specifically, virus RNA polymerases of RNA viruses free of virus genomic RNA.Type: ApplicationFiled: April 18, 2003Publication date: July 1, 2004Inventors: Ayae Honda, Akira Ishihama
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Publication number: 20040126865Abstract: Ribonuclease DNA coding for an amino acid sequence beginning with a residue of glutamine is introduced into a vector of pET22b(+) plasmid to form recombinant plasmid DNA that begins with a Pel B leader sequence. The recombinant plasmid DNA is used to transform an E.coli BL21(DE3) host. Signal peptidase enzyme present in the host cell cleaves the pelB leader sequence during signal processing and thereby allows the glutamine residue to autocyclize to pyroglutamic acid. In this way, the Ribonuclease can be produced directly, i.e. without a separate step of cleaving the initial N-terminal methionine residue.Type: ApplicationFiled: December 30, 2002Publication date: July 1, 2004Applicant: ALFACELL CORPORATIONInventor: Shailendra K. Saxena
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Publication number: 20040123339Abstract: The present invention is in the field of plant biochemistry. More specifically the invention relates to nucleic acid sequences from plant cells, in particular, nucleic acid sequences from maize, soybean and Arabidopsis thaliana associated with transcription factors. The invention encompasses nucleic acid molecules that encode proteins and fragments of proteins. In addition, the invention also encompasses proteins and fragments of proteins so encoded and antibodies capable of binding these proteins or fragments. The invention also relates to methods of using the nucleic acid molecules, proteins and fragments of proteins and antibodies, for example for genome mapping, gene identification and analysis, plant breeding, preparation of constructs for use in plant gene expression and transgenic plants.Type: ApplicationFiled: August 6, 2001Publication date: June 24, 2004Inventors: Timothy W. Conner, Gregory R. Heck, Jingdong Liu
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Publication number: 20040123338Abstract: Expressed Sequence Tags (ESTs) isolated from cotton are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.Type: ApplicationFiled: December 8, 2000Publication date: June 24, 2004Inventor: Karen L. Fincher
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Publication number: 20040123340Abstract: Expressed Sequence Tags (ESTs) isolated from cotton are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.Type: ApplicationFiled: December 12, 2001Publication date: June 24, 2004Inventors: Jill Deikman, Paul C.C. Feng, Karen L. Fincher, Todd E. Ziegler
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Publication number: 20040121361Abstract: The invention provides human nucleic acid-associated proteins (NAAP) and polynucleotides which identify and encode NAAP. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating, or preventing disorders associated with aberrant expression of NAAP.Type: ApplicationFiled: September 29, 2003Publication date: June 24, 2004Inventors: Junming Yang, April J.A. Hafalia, Neil Burford, Danniel B. Nguyen, Shanya D. Becha, Y. Tom Tang, Thomas W. Richardson, Henry Yue, Bridget A. Warren, Brooke M. Emerling, Mariah R. Baughn, Jennifer A. Griffin, Vicki S. Elliott, Narinder K. Chawla, Preeti G. Lal, Yalda Azimzai, Patricia M. Mason, Anna M. Chinn, Huibin Yue
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Publication number: 20040115786Abstract: A biosensor device for detecting small molecules analytes is provided. The device employs a first class of molecules, e.g., protein that binds to both the analyte and a second class of molecules, e.g., nucleic acid. The binding of the protein to the analyte and nucleic acid can be mutually exclusive, and the presence of analyte in a sample results in a detectable displacement of protein from nucleic acid. Alternatively, binding of the protein to the nucleic acid can depend on the presence of analyte in the sample. In a specific embodiment, either the protein or nucleic acid is immobilized on a solid phase support. An arsenic detection system is exemplified. An ArsR binding sequence from the E. coli ars operon is immobilized on a gold-plated surface. ArsR protein binds to the DNA in the absence of arsenic, and is released in the presence of sodium arsenate or phenylarsine oxide.Type: ApplicationFiled: September 30, 2003Publication date: June 17, 2004Inventor: Lance G. Laing
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Publication number: 20040115715Abstract: We have now discovered that mammals, have a DNA gene analogous to that existing in bacteria. MSH5 defects or alterations in this mismatch repair pathway in a mammal, such as a human can be diagnostic of a predisposition to cancer, and prognostic for a particular cancer.Type: ApplicationFiled: October 7, 2003Publication date: June 17, 2004Applicant: Dana-Farber Cancer Institute, Inc.Inventors: Richard Kolodner, Nena Winand
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Publication number: 20040115639Abstract: The invention relates to a composition comprising a first modified thermostable enzyme exhibiting 3′exonuclease activity but essentially no DNA polymerase activity and a second modified thermostable enzyme exhibiting DNA polymerase activity, whereas the fidelity of an amplification process is enhanced by the use of the composition in an amplification process in comparison to the use of the single second enzyme in an amplification process and, whereas said first and said second modified thermostable enzyme is reversibly modified by an inhibiting agent which results in essentially complete inactivation of enzyme activity, wherein incubation of said first and said second modified thermostable enzyme in an aqueous buffer at alkaline pH at a temperature less than 25° C. for 20 minutes results in no significant increase in the activity of said first and said second modified thermostable enzyme, wherein incubation at a temperature greater than 50° C.Type: ApplicationFiled: December 11, 2002Publication date: June 17, 2004Applicant: Roche Molecular Systems, Inc.Inventors: Harald Sobek, Michael Greif
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Publication number: 20040115661Abstract: A plasmid has been isolated from Rhodococcus erythropolis strain AN12 comprising a unique replication protein. The replication protein may be used in a variety of cloning and expression vectors and particularly in shuttle vectors for the expression of heterologous genes in Rhodococcus sp.Type: ApplicationFiled: September 4, 2003Publication date: June 17, 2004Inventors: Michael G. Bramucci, Qiong Cheng, Kristy N. Kostichka, Jean-Francois Tomb
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Publication number: 20040110265Abstract: A method of recovering an enzyme activity, such as reverse transcriptase (RT) activity, from enveloped viruses, such as HIV, in a biological sample containing a non-protected enzymes, is described. A cysteine-modifying substance is used to destroy the activity of the non-protected enzymes, followed by removal of the enveloped virus particles or inactivation of the cysteine-modifying substance with a chemical. Then the virus envelope is lysed and the released enzymes are recovered. Commercial packages containing written and/or data carrier instructions and some chemicals for performing laboratory steps for recovery of an enzyme activity from enveloped viruses, are also disclosed.Type: ApplicationFiled: October 3, 2003Publication date: June 10, 2004Inventors: Clas Kallander, Simon Gronowitz, Tommy Gatu
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Publication number: 20040110210Abstract: The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.Type: ApplicationFiled: September 26, 2003Publication date: June 10, 2004Inventors: Michael E. O'Donnell, Alexander Yuzhakov, Olga Yurieva, David Jeruzalmi, Irina Bruck, John Kuriyan
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Publication number: 20040111768Abstract: A transgenic plant transformed by a transcription factor stress-related protein (TFSRP) coding nucleic acid, wherein expression of the nucleic acid sequence in the plant results in increased tolerance to environmental stress as compared to a wild type variety of the plant. Also provided are agricultural products, including seeds, produced by the transgenic plants. Also provided are isolated TFSRP, and isolated nucleic acid coding TFSRP, and vectors and host cells containing the latter. Further provided are methods of producing transgenic plants expressing TFSRP, methods of increasing expression of other genes of interest using the TFSRP, methods of identifying novel TFSRP, and methods of modifying the expression of TFSRP in plants.Type: ApplicationFiled: October 29, 2002Publication date: June 10, 2004Inventors: Oswaldo da Costa e Silva, Nocha van Thielen, Ruoying Chen
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Patent number: 6743968Abstract: Genetic constructs and methods are disclosed for the production, maintenance and control of transgenes in transgenic eukaryotic organisms that undergo meiosis in which pollen or sperm can be outcrossed; this includes: transgenic animals, plant cells, plant tissues and whole plants. More specifically, this invention relates to the control of transgene transmission by male and/or female gametes or gametophytes using a gametophytic sterility trait (GST). The genetic constructs and methodologies of the present invention provide the ability to control the undesired spread of transgenes. In addition, this invention also provides the tools and methodologies to enrich a plant or other eukaryotic genome for dispersed and/or stable transposition events.Type: GrantFiled: February 28, 2001Date of Patent: June 1, 2004Assignee: Yale UniversityInventors: Stephen L. Dellaporta, Maria A. Moreno
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Publication number: 20040101943Abstract: The invention provides human nucleic acid modification enzymes (NAMO) and polynucleotides which identify and encode NAMO. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating, or preventing disorders associated with aberrant expression of NAMO.Type: ApplicationFiled: April 29, 2003Publication date: May 27, 2004Applicant: Incyte CorporationInventors: Mariah R. Baughn, Henry Yue, Yan Lu, Li Ding, Y Tom Tang, Ameena R Gandhi, April J A Hafalia, Preeti G Lal
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Publication number: 20040101883Abstract: The invention provides human adenylyl and guanylyl cyclases (ADGUC) and polynucleotides which identify and encode ADGUC. The invention alsoprovides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating, or preventing disorders associated with aberrant expression of ADGUC.Type: ApplicationFiled: September 19, 2003Publication date: May 27, 2004Inventors: Li Ding, Narinder K Chawla, Mariah R Baughn, Vicki S Elliott, Chandra S Arvizu, Yan Lu, Rajagopal Gururajan
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Publication number: 20040096858Abstract: The invention provides human adenylyl and guanylyl cyclases (ADGUC) and polynucleotides which identify and encode ADGUC. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The invention also provides methods for diagnosing, treating, or preventing disorders associated with aberrant expression of ADGUC.Type: ApplicationFiled: June 19, 2003Publication date: May 20, 2004Inventors: Mariah R. Baughn, Catherine M. Tribouley, Narinder K. Chawla, Yan Lu
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Publication number: 20040098760Abstract: The invention provides isolated nucleic acids and their encoded proteins that act as cell transcription inhibitors and methods of use thereof. The invention further provides expression cassettes, transformed host cells, transgenic plants and plant parts, and antibody compositions.Type: ApplicationFiled: September 30, 2003Publication date: May 20, 2004Applicants: Pioneer Hi-Bred International, Inc., E.I. du Pont de Nemours and CompanyInventors: Yumin Tao, William J. Gordon-Kamm, Bo Shen, Keith S. Lowe, Olga N. Danilevskaya, Pramod B. Mahajan, Jan Antoni Rafalski, Hajime Sakai, Theodore M. Klein
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Publication number: 20040098769Abstract: Methods and compositions for modulating DNA metabolism are provided. Nucleotide and amino acid sequences encoding a maize replication protein A subunits are provided. The sequences can be used in expression cassettes for modulating DNA replication, DNA repair, and recombination.Type: ApplicationFiled: February 21, 2003Publication date: May 20, 2004Applicant: Pioneer Hi-Bred International, Inc.Inventor: Pramod B. Mahajan
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Use of consensus sequences for targeted homologous gene isolation and recombination in gene families
Publication number: 20040091885Abstract: The invention relates to compositions and methods for targeting sequence modifications in one or more genes of a related family of genes using enhanced homologous recombination techniques. These techniques may be used to create animal or plant models of disease as well as to identify new targets for drug or pathogen screening.Type: ApplicationFiled: February 24, 2003Publication date: May 13, 2004Applicant: Pangene CorporationInventors: Sushma Pati, David A. Zarling, Christopher W. Lehman, Hong Zeng -
Publication number: 20040091886Abstract: A novel method for producing recombinant polynucleotides in vitro is provided. This method entails the treatment of heteroduplex DNA sequences with a nuclease (preferably, DNase I) and a polymerase (preferably, DNA polymerase I), the enzymes primarily involved in nick translation. The results achieved using this process are superior to that achieved by previous in vivo recombination efforts utilizing specific DNA repair systems.Type: ApplicationFiled: March 20, 2003Publication date: May 13, 2004Inventors: Jeffrey C. Moore, Jeffrey Bernstein, James K. McCarthy
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Publication number: 20040091892Abstract: A restriction endonuclease having one DNA binding site is proposed, synthesized from a restriction endonuclease that has one C-terminal domain and one N-terminal domain and two DNA binding sites, by proteolytic cleavage into the two domains or by cloning the gene segment that codes for the domains and expression of the domains and selection of the endonucleolytic domains having one DNA binding site. In addition, a method of synthesis of the restriction endonuclease and its use are claimed.Type: ApplicationFiled: April 29, 2003Publication date: May 13, 2004Inventors: Merlind Mucke, Monika Reuter, Detlev Kruger
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Publication number: 20040091911Abstract: In accordance with the present invention, there is provided a DNA (deoxyribonucleic acid) fragment which encodes the MmeI type II restriction endonuclease enzyme.Type: ApplicationFiled: July 10, 2003Publication date: May 13, 2004Inventors: Richard D. Morgan, Tanya Bhatia, Theodore Davis, Lindsay Lovasco
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Publication number: 20040091873Abstract: The invention relates to the generation and characterization of archaeal DNA polymerase mutants with reduced base analog detection activity. The invention further provides for archaeal DNA polymerase mutants with reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or 3′-5′ exonuclease activity. The invention also discloses methods and applications of DNA polymerases with reduced base analog detection activity.Type: ApplicationFiled: November 18, 2002Publication date: May 13, 2004Applicant: StratageneInventors: Joseph A. Sorge, Holly Hogrefe, Madhushree Ghosh
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Publication number: 20040091955Abstract: The present invention is a simplified, highly-purified, processive translation system that does not require the addition of translation factors EF-P, W, W2 or rescue. A new translation process offers new, potentially improved, routes to all peptides, proteins and peptidomimetics currently synthesized by alternative routes.Type: ApplicationFiled: January 25, 2002Publication date: May 13, 2004Inventors: Anthony C. Forster, Stephen C. Blacklow
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Publication number: 20040091907Abstract: The present invention relates to diagnosing abnormal cell proliferation in biological samples and screening for drugs which inhibit, reduce or abolish cell growth, especially tumorigenic cell growth, by detecting a phosphovariant isoform of a guanine nucleotide exchange factor biomarker, such as the novel GEF-H1S.Type: ApplicationFiled: July 2, 2003Publication date: May 13, 2004Applicant: Sugen, Inc.Inventors: Tod R. Smeal, Marinella G. Callow, Bahija Jallal, Sergey Zozulya, Mikhail L. Gishizky
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Publication number: 20040087525Abstract: A novel polypeptide-human RNA binding protein 19, the polynucleotide encoding it and a method producing the polypeptide by DNA recombinant technology. The present invention further discloses a method using the peptide for treating various disorders, e.g., protein metabolism disorder, embryonic deformative, various tumors, and immunological diseases. The protein is also suitable for human antisenescence research. The present invention also discloses an antagonist of the polypeptide and its therapeutic application. The present invention further discloses the use of the polynucleotide encoding the novel human RNA binding protein 19.Type: ApplicationFiled: December 18, 2002Publication date: May 6, 2004Inventors: Yumin Mao, Yi Xie
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Publication number: 20040086890Abstract: The invention relates to the generation and characterization of archaeal DNA polymerase mutants with reduced base analog detection activity. The invention further provides for archaeal DNA polymerase mutants with reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or 3′-5′ exonuclease activity. The invention also discloses methods and applications of DNA polymerases with reduced base analog detection activity.Type: ApplicationFiled: April 7, 2003Publication date: May 6, 2004Applicant: StratageneInventors: Joseph A. Sorge, Holly H. Hogrefe, Madhushree Ghosh
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Publication number: 20040086889Abstract: The present invention relates to a DNA polymerase immobilized by covalent bonding. More particularly, the present invention relates to an immobilized DNA polymerase whose activity is maximally preserved by masking the active site of the DNA polymerase and optimizing interaction of the masked molecule to the substrate material. In one embodiment, the average activity of the immobilized DNA polymerase is more than about 10% relative to that of the solution phase DNA polymerase. Further provided by the invention are methods and kits for performing polymerase chain reactions (PCR).Type: ApplicationFiled: April 2, 2003Publication date: May 6, 2004Inventors: Hyun Jin Hwang, Jeong Hee Kim
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Publication number: 20040081963Abstract: This invention provides Sso7-polymerase conjugates that exhibit improved activity in a polymerase reaction.Type: ApplicationFiled: October 23, 2002Publication date: April 29, 2004Applicant: MJ Bioworks IncorporatedInventor: Yan Wang
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Publication number: 20040081995Abstract: The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polymerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.Type: ApplicationFiled: September 26, 2003Publication date: April 29, 2004Inventors: Michael E. O'Donnell, Alexander Yuzhakov, Olga Yurieva, David Jeruzalmi, Irina Bruck, John Kuriyan